LIF cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIF
Entrez Gene ID	399503
Full Name	LIF, interleukin 6 family cytokine
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 14 Map Location: 14\|14

mRNA and Protein(s)

mRNA	Protein	Name
XM_013983026.2	XP_013838480.1	leukemia inhibitory factor isoform X1
XM_013983025.1	XP_013838479.1	leukemia inhibitory factor isoform X1
XM_013983026.1	XP_013838480.1	leukemia inhibitory factor isoform X1
NM_214402.2	NP_999567.1	leukemia inhibitory factor precursor
XM_005670785.2	XP_005670842.1	leukemia inhibitory factor isoform X1
XM_013983025.2	XP_013838479.1	leukemia inhibitory factor isoform X1
XM_005670785.3	XP_005670842.1	leukemia inhibitory factor isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3.
Dang-Nguyen TQ, Haraguchi S, Kikuchi K, Somfai T, Bod? S, Nagai T
Molecular reproduction and development81(3)230-9(2014 Mar)

Cell cycle synchronization of leukemia inhibitory factor (LIF)-dependent porcine-induced pluripotent stem cells and the generation of cloned embryos.
Yuan Y, Lee K, Park KW, Spate LD, Prather RS, Wells KD, Roberts RM
Cell cycle (Georgetown, Tex.)13(8)1265-76(2014)

Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs.
Blitek A, Morawska E, Ziecik AJ
Theriogenology78(5)951-64(2012 Sep)

Reprogramming pig fetal fibroblasts reveals a functional LIF signaling pathway.
Thomson AJ, Pierart H, Meek S, Bogerman A, Sutherland L, Murray H, Mountjoy E, Downing A, Talbot R, Sartori C, Whitelaw CB, Freeman TC, Archibald AL, Burdon T
Cellular reprogramming14(2)112-22(2012 Apr)

Effect of polymorphism in the leukemia inhibitory factor gene on litter size in Large White pigs.
Lin HC, Liu GF, Wang AG, Kong LJ, Wang XF, Fu JL
Molecular biology reports36(7)1833-8(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studied effect of recombinant porcine leukemia inhibitory factor on in vitro maturation of porcine oocytes; p-STAT3 markedly increased in both cumulus cells and oocytes cultured in pLIF-supplemented media.
Title: Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3.

Cell cycle synchronization of leukemia inhibitory factor (LIF)-dependent porcine-induced pluripotent stem cells and the generation of cloned embryos.
Title: Cell cycle synchronization of leukemia inhibitory factor (LIF)-dependent porcine-induced pluripotent stem cells and the generation of cloned embryos.

LIF and IL-6 are important components of embryo-uterine interactions during early pregnancy in the pig, and may contribute to successful conceptus implantation.
Title: Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs.

Reprogramming pig fetal fibroblasts reveals a functional LIF signaling pathway.
Title: Reprogramming pig fetal fibroblasts reveals a functional LIF signaling pathway.

The results indicated that the B allele at SNP locus LIF1 and the A allele at SNP locus LIF2 seem to have advantageous effects on litter size.
Title: Effect of polymorphism in the leukemia inhibitory factor gene on litter size in Large White pigs.

The following LIF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe21839	NM_214402.2 Latest version!	Sus scrofa LIF interleukin 6 family cytokine (LIF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe21839	XM_013983025.2 Latest version!	Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe21839	XM_013983026.2 Latest version!	Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe21839	XM_005670785.3 Latest version!	Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OSe21839	XM_013983026.1	Sus scrofa leukemia inhibitory factor (LIF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe21839	XM_013983025.1	Sus scrofa leukemia inhibitory factor (LIF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe21839	XM_005670785.2	Sus scrofa leukemia inhibitory factor (LIF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIF ( NM_214402.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999567.1 Sus scrofa LIF interleukin 6 family cytokine (LIF), mRNA.

Price & Availability↑

CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	NM_214402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-28
Organism	Sus scrofa(pig)
Product	leukemia inhibitory factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY585336.1. On Jun 3, 2007 this sequence version replaced NM_214402.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY585336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01893934, SAMN01893938 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999567.1
CDS	1..609
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	NM_214402.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa LIF interleukin 6 family cytokine (LIF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214402.2

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIF ( XM_013983026.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013838480.1 Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	XM_013983026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leukemia inhibitory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013983026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013838480.1
CDS	1853..2461
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	XM_013983026.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013983026.2

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIF ( XM_013983025.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013838479.1 Sus scrofa leukemia inhibitory factor (LIF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	XM_013983025.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	leukemia inhibitory factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003611968.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013838479.1
CDS	363..971
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	XM_013983025.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa leukemia inhibitory factor (LIF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013983025.1

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIF ( XM_013983026.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013838480.1 Sus scrofa leukemia inhibitory factor (LIF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	XM_013983026.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	leukemia inhibitory factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003611968.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013838480.1
CDS	1826..2434
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	XM_013983026.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa leukemia inhibitory factor (LIF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013983026.1

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIF ( XM_005670785.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005670842.1 Sus scrofa leukemia inhibitory factor (LIF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	XM_005670785.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	leukemia inhibitory factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003611968.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005670785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005670842.1
CDS	780..1388
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	XM_005670785.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa leukemia inhibitory factor (LIF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005670785.2

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIF ( XM_013983025.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013838479.1 Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X2, mRNA.

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CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	XM_013983025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leukemia inhibitory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013983025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013838479.1
CDS	2126..2734
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	XM_013983025.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013983025.2

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIF ( XM_005670785.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005670842.1 Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe21839
Clone ID Related Accession (Same CDS sequence)	XM_013983026.2 , XM_013983025.1 , XM_013983026.1 , NM_214402.2 , XM_005670785.2 , XM_013983025.2 , XM_005670785.3
Accession Version	XM_005670785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leukemia inhibitory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005670785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005670842.1
CDS	1436..2044
Translation	MKVLAAGVVPLLLVLHWKHGAGSPLSITPVNATCATRHPCHSNLMNQIKNQLAQLNSSANALFILYYTAQGEPFPNNLDKLCGPNVTNFPPFHANGTEKARLVELYRIIAYLGASLGNITRDQRSLNPGAVNLHSKLNATADSMRGLLSNVLCRLCNKYHVAHVDVAYGPDTSGKDVFQKKKLGCQLLGKYKQVISVLARAF

Target ORF information:

RefSeq Version	XM_005670785.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa LIF, interleukin 6 family cytokine (LIF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005670785.3

ORF Insert Sequence:

ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGCTGG TTCTGCACTG GAAACACGGG 
GCAGGGAGCC CCCTTTCCAT CACTCCTGTC AATGCCACCT GTGCCACACG TCACCCATGT 
CACAGCAACC TCATGAACCA GATCAAGAAC CAGCTGGCGC AGCTCAACAG CAGTGCCAAC 
GCCCTCTTTA TTCTCTACTA CACAGCCCAG GGGGAGCCAT TTCCCAACAA CTTGGACAAG 
CTGTGTGGCC CCAACGTGAC CAACTTCCCG CCCTTCCACG CCAACGGCAC CGAGAAGGCC 
CGGCTGGTGG AGCTGTACCG CATCATCGCC TACCTTGGCG CCTCCCTGGG CAACATCACG 
CGGGACCAAA GGAGCCTCAA TCCTGGTGCT GTGAACCTGC ACTCCAAGCT GAACGCCACG 
GCGGACAGCA TGCGAGGCCT CCTCAGCAAT GTGCTCTGCC GCCTGTGCAA CAAGTACCAC 
GTGGCCCATG TGGATGTGGC CTACGGCCCC GACACCTCGG GCAAGGACGT CTTCCAGAAG 
AAGAAGCTGG GCTGTCAGCT CCTGGGGAAG TATAAGCAGG TCATCTCTGT GCTAGCCCGG 
GCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3.
Molecular reproduction and development81(3)230-9(2014 Mar)
Dang-Nguyen TQ,Haraguchi S,Kikuchi K,Somfai T,Bod? S,Nagai T

Cell cycle synchronization of leukemia inhibitory factor (LIF)-dependent porcine-induced pluripotent stem cells and the generation of cloned embryos.
Cell cycle (Georgetown, Tex.)13(8)1265-76(2014)
Yuan Y,Lee K,Park KW,Spate LD,Prather RS,Wells KD,Roberts RM

Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs.
Theriogenology78(5)951-64(2012 Sep)
Blitek A,Morawska E,Ziecik AJ

Reprogramming pig fetal fibroblasts reveals a functional LIF signaling pathway.
Cellular reprogramming14(2)112-22(2012 Apr)
Thomson AJ,Pierart H,Meek S,Bogerman A,Sutherland L,Murray H,Mountjoy E,Downing A,Talbot R,Sartori C,Whitelaw CB,Freeman TC,Archibald AL,Burdon T

Effect of polymorphism in the leukemia inhibitory factor gene on litter size in Large White pigs.
Molecular biology reports36(7)1833-8(2009 Sep)
Lin HC,Liu GF,Wang AG,Kong LJ,Wang XF,Fu JL

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LIF cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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