| Gene Symbol | ANPEP |
| Entrez Gene ID | 397520 |
| Full Name | alanyl aminopeptidase, membrane |
| Synonyms | APN,PEPN |
| Gene Type | protein-coding |
| Organism | Sus scrofa(pig) |
| Genome |
ORF » Species Summary » Sus scrofa » ANPEP cDNA ORF clone
| Gene Symbol | ANPEP |
| Entrez Gene ID | 397520 |
| Full Name | alanyl aminopeptidase, membrane |
| Synonyms | APN,PEPN |
| Gene Type | protein-coding |
| Organism | Sus scrofa(pig) |
| Genome |
| mRNA | Protein | Name |
|---|---|---|
| XM_005653523.3 | XP_005653580.1 | aminopeptidase N isoform X2 |
| XM_021098304.1 | XP_020953963.1 | aminopeptidase N isoform X1 |
| XM_005653524.2 | XP_005653581.1 | aminopeptidase N isoform X1 |
| XM_005653523.2 | XP_005653580.1 | aminopeptidase N isoform X1 |
| XM_005653524.3 | XP_005653581.1 | aminopeptidase N isoform X2 |
| NM_214277.1 | NP_999442.1 | aminopeptidase N |
Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry.
Li W, Luo R, He Q, van Kuppeveld FJM, Rottier PJM, Bosch BJ
Virus research2356-13(2017 May)
Porcine aminopeptidase N is not a cellular receptor of porcine epidemic diarrhea virus, but promotes its infectivity via aminopeptidase activity.
Shirato K, Maejima M, Islam MT, Miyazaki A, Kawase M, Matsuyama S, Taguchi F
The Journal of general virology97(10)2528-2539(2016 Oct)
Activity profiling of aminopeptidases in cell lysates using a fluorogenic substrate library.
Byzia A, Szeffler A, Kalinowski L, Drag M
Biochimie12231-7(2016 Mar)
Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains.
Deng F, Ye G, Liu Q, Navid MT, Zhong X, Li Y, Wan C, Xiao S, He Q, Fu ZF, Peng G
Viruses8(3)55(2016 Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
pAPN is not a functional receptor for porcine epidemic diarrhea virus, but promotes the infection of PEDV through its protease activity.
Title: Porcine aminopeptidase N is not a cellular receptor of porcine epidemic diarrhea virus, but promotes its infectivity via aminopeptidase activity.
Data indicate that fluorogenic substrates can be successfully used to identify aminopeptidase N and to measure their activity in cell lysates.
Title: Activity profiling of aminopeptidases in cell lysates using a fluorogenic substrate library.
The C-terminal domain of the S1 domain of porcine epidemic diarrhea virus is bound to swine pAPN.
Title: Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains.
SPC subdomain of APN plays a key role in cell entry of PEDV and its expression permits PEDV growth
Title: Identification of the functional domain of the porcine epidemic diarrhoea virus receptor.
Porcine epidemic diarrhea virus recognizes protein receptor aminopeptidase N from pig and human and sugar coreceptor N-acetylneuraminic acid.
Title: Receptor usage and cell entry of porcine epidemic diarrhea coronavirus.
The following ANPEP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANPEP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OSe00660 | |
| Clone ID Related Accession (Same CDS sequence) | NM_214277.1 | |
| Accession Version | NM_214277.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-11-15 | |
| Organism | Sus scrofa(pig) | |
| Product | aminopeptidase N | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z29522.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z29522.1, AK345090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02584787 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_999442.1 |
| CDS | 46..2937 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_214277.1 |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSe13952 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005653523.3 , XM_005653524.2 , XM_005653523.2 , XM_005653524.3 | |
| Accession Version | XM_005653523.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-05-12 | |
| Organism | Sus scrofa(pig) | |
| Product | aminopeptidase N isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005653523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005653580.1 |
| CDS | 321..3212 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005653523.3 |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSe13952 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005653523.3 , XM_005653524.2 , XM_005653523.2 , XM_005653524.3 | |
| Accession Version | XM_005653524.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-10 | |
| Organism | Sus scrofa(Pig) | |
| Product | aminopeptidase N isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003300254.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005653524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005653581.1 |
| CDS | 278..3169 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005653524.2 |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSe13952 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005653523.3 , XM_005653524.2 , XM_005653523.2 , XM_005653524.3 | |
| Accession Version | XM_005653523.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-09-10 | |
| Organism | Sus scrofa(Pig) | |
| Product | aminopeptidase N isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003300254.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005653523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005653580.1 |
| CDS | 312..3203 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005653523.2 |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSe13952 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005653523.3 , XM_005653524.2 , XM_005653523.2 , XM_005653524.3 | |
| Accession Version | XM_005653524.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-05-12 | |
| Organism | Sus scrofa(pig) | |
| Product | aminopeptidase N isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005653524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005653581.1 |
| CDS | 319..3210 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005653524.3 |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OSe222504 | |
| Clone ID Related Accession (Same CDS sequence) | XM_021098304.1 | |
| Accession Version | XM_021098304.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-05-12 | |
| Organism | Sus scrofa(pig) | |
| Product | aminopeptidase N isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_020953963.1 |
| CDS | 312..3365 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_021098304.1 |
1 | ATGGCCAAGG GATTCTACAT TTCCAAGGCC CTGGGCATCC TGGGCATCCT CCTCGGCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry. |
Porcine aminopeptidase N is not a cellular receptor of porcine epidemic diarrhea virus, but promotes its infectivity via aminopeptidase activity. |
Activity profiling of aminopeptidases in cell lysates using a fluorogenic substrate library. |
Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains. |