CHGB cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHGB
Entrez Gene ID	397154
Full Name	chromogranin B
Synonyms	CGB
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 17 Map Location: 17\|17

mRNA and Protein(s)

mRNA	Protein	Name
NM_214081.2	NP_999246.2	secretogranin-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

Linkage mapping of the porcine chromogranin B (CHGB) gene to chromosome 17.
Kim JG, Nonneman D, Vallet JL, Rohrer GA, Christenson RK
Animal genetics36(2)168(2005 Apr)

Presence and release of the chromogranin B-derived secretolytin-like peptide KR-11 from the porcine spleen.
Depreitere J, Wang Z, Liang F, Coen E, Nouwen EJ
Regulatory peptides122(2)99-107(2004 Oct)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Cellular expression and specific intragranular localization of chromogranin A, chromogranin B, and synaptophysin during ontogeny of pancreatic islet cells: an ultrastructural study.
Lukinius A, Stridsberg M, Wilander E
Pancreas27(1)38-46(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression and localization of chromogranin A (CgA), chromogranin B (CgB), synaptophysin, and insulin were ultrastructurally studied with the immunogold technique in porcine and human pancreatic islet neuroendocrine cells
Title: Cellular expression and specific intragranular localization of chromogranin A, chromogranin B, and synaptophysin during ontogeny of pancreatic islet cells: an ultrastructural study.

The following CHGB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHGB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe28517	NM_214081.2 Latest version!	Sus scrofa chromogranin B (CHGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHGB ( NM_214081.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999246.2 Sus scrofa chromogranin B (CHGB), mRNA.

Price & Availability↑

CloneID	OSe28517
Clone ID Related Accession (Same CDS sequence)	NM_214081.2
Accession Version	NM_214081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Sus scrofa(pig)
Product	secretogranin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY613916.1. On Jun 13, 2004 this sequence version replaced NM_214081.1. ##Evidence-Data-START## Transcript exon combination :: AY613916.1, AK343515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487410, SAMEA1487420 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCGG CGGTGCTTCT CGGCTTCCTG GGAGCTACTG TGGTGGCCGC TGTCAGTTCT 
ATGCCAGTGG ACAACAGGAA CCACAATGAA GAAATGGTGG CTCACTGCAT CATCGAGGTC 
CTTTCAAATG CCCTGTCGAA GTCCAATGCT CCTCCCATCA CTCCTGAGTG CAGACAAGTC 
CTGAAGAAAG GTGGAAAAGA GGTCAAAGAT GAAGAGAAAG GTGCAAATGA GAACACAAGG 
TTTGAAGTGA GATTGTTAAG AGACCCAGCT GACACCTCCG AAACCCAGAG GCCCTCCGGT 
GGTAGGGAGG GCGCAGAAGC CCCATCGGAG GACACCCAAG GCCCCCCAGG GGCGGACGTA 
GAGGGCGGCG GGCACAGCCG AGAGGGGGCA GGCAAGCCCC GAGGGGGCCC CTACTCCTCT 
GACAACCAAG TGGCCAAGGA AGGCAAGACA CGCCATTCTG AGAAGAGCGA GGGACAGGAC 
AGGGAAGAAG AAGAGGGAGA GAAATATCAG AAAAGGGAGC GTGGGGAAGA GGGCAGCGAG 
GAGCGACACC TCCAAGAGCC GGGAGAGACG CAAACTGCTT TTCTCAACCA AGGAAACCGG 
GCTACAGCTA AGAAAAAAGA GGAGTTCGAG TCCAGGTACG ATGCACACTC TGCTGGGGGC 
CCTGCGAAGA CACACAGCCG GGAAAGGAGC AGCCAGGAGA GTGGAGAGGA GACCGGGAGC 
CAGGACGCGG CACCCGGAGA GCCTGAAAGC CCACCCGAGG GTCAGGAGGC ACCTGAGGAA 
AGCCAGGAAG ATGCCAGCCT TGAGGTGGAC AAACGACGCT GGAGGCCGAG GCATCACCAC 
GGGAGGAGCA GGCCGGACAG AGCCCCTCAG GAAGGGAGTC CTCCCCCCGA GGAGAGAGGA 
CACCCCCGCG AGGAATCTGA GGAGTCAGTG TTGGGCCTGG CCAGCCCCGG GGGACAGAGA 
ACCCGCCATC CTACCCACTA CCCAGCTTCC GAAGAAGAAG CCGAACACGG GGAAGAAGTG 
AGGAGTTCCT CCTCGGTCCA GGCTCCTGGG GACCTGGTGG GGGCACGATA CGGGGGCAGA 
GGGGGTGCGG AACACCGGGC TGCAAGACGT CCCGGCGAGG AGAGCCCAGA GCAGGACAGG 
AGGGTGGGCG CCAGCTCGGA GCTGAGGAAT AAGGGGCCCG GACACAGTGA GGAAAGTGAG 
GAAGAGAGAG GCGACGAGGG GCAGCGGCAC CACCGAGCCT GGGCAGGGGG GCCACGTGCC 
GACGCCCCTC CTGACCCGGC AGAAGAGGAA CAGTTCTGGG GTGAAGCACA CCGCCCTGTT 
CCAGAAAGCC AGGTGGACGA GGCAAGGCGC CACCCACAGG CCAAGCTGAG AAATTACCTC 
AACCGCGGTG AGGAGAATGG GGAGGAAGGA GCCCCAGGCA GGTGGCAGCA GCCGGAGGAC 
CTGCAAGGCC CTGGGGAGCA CGCTGCAGAA GCCAGGCTTC CAGGCAAACC CTATGCTGCC 
CATCGCATCC CTGAGAAGAA GAGATTAGGG GAGCTACTCA ATCCATACTA CGTCCCACCC 
CAGTGGGAGA GCAGCCATTT CGAGAGGAAG GACAACGTGG GTGACAATTT TCTTGAGGGT 
GAAGATGCAA ATGGGGTGAC CTTGAACGAG AAGACTTTCT TCCCGGAATA CAGCTATGAC 
TGGTGGGAGA AAAAGCCCTT TGAGGAGGAT GTAAACTGGG GATATGAGGA GAGAAACCTG 
GCCCCCAAAC TGGATCTGAA GAGGCAGTAT GACAGAGTGG CTGAACTGGA CCAGCTTCTT 
CATTACAGGA AGAAGTCAGC TGAGTTTCCA GACTTCTATG ACTCCGGGGA GCACCTGAGT 
CCACGCCACG CAGCAGAGAG TGAGAAGGAG AGGGCCGGCC AAGGAGTTCT GACAGAGGAG 
GAGGAAAAAG AACTCGAAAA TTTGGCTGCA ATGGATTTGG AACTACAGAA AATAGCTGAG 
AAGTTCAGTG GTAACCGAAG GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999246.2
CDS	102..2108
Translation	MQPAVLLGFLGATVVAAVSSMPVDNRNHNEEMVAHCIIEVLSNALSKSNAPPITPECRQVLKKGGKEVKDEEKGANENTRFEVRLLRDPADTSETQRPSGGREGAEAPSEDTQGPPGADVEGGGHSREGAGKPRGGPYSSDNQVAKEGKTRHSEKSEGQDREEEEGEKYQKRERGEEGSEERHLQEPGETQTAFLNQGNRATAKKKEEFESRYDAHSAGGPAKTHSRERSSQESGEETGSQDAAPGEPESPPEGQEAPEESQEDASLEVDKRRWRPRHHHGRSRPDRAPQEGSPPPEERGHPREESEESVLGLASPGGQRTRHPTHYPASEEEAEHGEEVRSSSSVQAPGDLVGARYGGRGGAEHRAARRPGEESPEQDRRVGASSELRNKGPGHSEESEEERGDEGQRHHRAWAGGPRADAPPDPAEEEQFWGEAHRPVPESQVDEARRHPQAKLRNYLNRGEENGEEGAPGRWQQPEDLQGPGEHAAEARLPGKPYAAHRIPEKKRLGELLNPYYVPPQWESSHFERKDNVGDNFLEGEDANGVTLNEKTFFPEYSYDWWEKKPFEEDVNWGYEERNLAPKLDLKRQYDRVAELDQLLHYRKKSAEFPDFYDSGEHLSPRHAAESEKERAGQGVLTEEEEKELENLAAMDLELQKIAEKFSGNRRG

Target ORF information:

RefSeq Version	NM_214081.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa chromogranin B (CHGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214081.2

ORF Insert Sequence:

ATGCAGCCGG CGGTGCTTCT CGGCTTCCTG GGAGCTACTG TGGTGGCCGC TGTCAGTTCT 
ATGCCAGTGG ACAACAGGAA CCACAATGAA GAAATGGTGG CTCACTGCAT CATCGAGGTC 
CTTTCAAATG CCCTGTCGAA GTCCAATGCT CCTCCCATCA CTCCTGAGTG CAGACAAGTC 
CTGAAGAAAG GTGGAAAAGA GGTCAAAGAT GAAGAGAAAG GTGCAAATGA GAACACAAGG 
TTTGAAGTGA GATTGTTAAG AGACCCAGCT GACACCTCCG AAACCCAGAG GCCCTCCGGT 
GGTAGGGAGG GCGCAGAAGC CCCATCGGAG GACACCCAAG GCCCCCCAGG GGCGGACGTA 
GAGGGCGGCG GGCACAGCCG AGAGGGGGCA GGCAAGCCCC GAGGGGGCCC CTACTCCTCT 
GACAACCAAG TGGCCAAGGA AGGCAAGACA CGCCATTCTG AGAAGAGCGA GGGACAGGAC 
AGGGAAGAAG AAGAGGGAGA GAAATATCAG AAAAGGGAGC GTGGGGAAGA GGGCAGCGAG 
GAGCGACACC TCCAAGAGCC GGGAGAGACG CAAACTGCTT TTCTCAACCA AGGAAACCGG 
GCTACAGCTA AGAAAAAAGA GGAGTTCGAG TCCAGGTACG ATGCACACTC TGCTGGGGGC 
CCTGCGAAGA CACACAGCCG GGAAAGGAGC AGCCAGGAGA GTGGAGAGGA GACCGGGAGC 
CAGGACGCGG CACCCGGAGA GCCTGAAAGC CCACCCGAGG GTCAGGAGGC ACCTGAGGAA 
AGCCAGGAAG ATGCCAGCCT TGAGGTGGAC AAACGACGCT GGAGGCCGAG GCATCACCAC 
GGGAGGAGCA GGCCGGACAG AGCCCCTCAG GAAGGGAGTC CTCCCCCCGA GGAGAGAGGA 
CACCCCCGCG AGGAATCTGA GGAGTCAGTG TTGGGCCTGG CCAGCCCCGG GGGACAGAGA 
ACCCGCCATC CTACCCACTA CCCAGCTTCC GAAGAAGAAG CCGAACACGG GGAAGAAGTG 
AGGAGTTCCT CCTCGGTCCA GGCTCCTGGG GACCTGGTGG GGGCACGATA CGGGGGCAGA 
GGGGGTGCGG AACACCGGGC TGCAAGACGT CCCGGCGAGG AGAGCCCAGA GCAGGACAGG 
AGGGTGGGCG CCAGCTCGGA GCTGAGGAAT AAGGGGCCCG GACACAGTGA GGAAAGTGAG 
GAAGAGAGAG GCGACGAGGG GCAGCGGCAC CACCGAGCCT GGGCAGGGGG GCCACGTGCC 
GACGCCCCTC CTGACCCGGC AGAAGAGGAA CAGTTCTGGG GTGAAGCACA CCGCCCTGTT 
CCAGAAAGCC AGGTGGACGA GGCAAGGCGC CACCCACAGG CCAAGCTGAG AAATTACCTC 
AACCGCGGTG AGGAGAATGG GGAGGAAGGA GCCCCAGGCA GGTGGCAGCA GCCGGAGGAC 
CTGCAAGGCC CTGGGGAGCA CGCTGCAGAA GCCAGGCTTC CAGGCAAACC CTATGCTGCC 
CATCGCATCC CTGAGAAGAA GAGATTAGGG GAGCTACTCA ATCCATACTA CGTCCCACCC 
CAGTGGGAGA GCAGCCATTT CGAGAGGAAG GACAACGTGG GTGACAATTT TCTTGAGGGT 
GAAGATGCAA ATGGGGTGAC CTTGAACGAG AAGACTTTCT TCCCGGAATA CAGCTATGAC 
TGGTGGGAGA AAAAGCCCTT TGAGGAGGAT GTAAACTGGG GATATGAGGA GAGAAACCTG 
GCCCCCAAAC TGGATCTGAA GAGGCAGTAT GACAGAGTGG CTGAACTGGA CCAGCTTCTT 
CATTACAGGA AGAAGTCAGC TGAGTTTCCA GACTTCTATG ACTCCGGGGA GCACCTGAGT 
CCACGCCACG CAGCAGAGAG TGAGAAGGAG AGGGCCGGCC AAGGAGTTCT GACAGAGGAG 
GAGGAAAAAG AACTCGAAAA TTTGGCTGCA ATGGATTTGG AACTACAGAA AATAGCTGAG 
AAGTTCAGTG GTAACCGAAG GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

Linkage mapping of the porcine chromogranin B (CHGB) gene to chromosome 17.
Animal genetics36(2)168(2005 Apr)
Kim JG,Nonneman D,Vallet JL,Rohrer GA,Christenson RK

Presence and release of the chromogranin B-derived secretolytin-like peptide KR-11 from the porcine spleen.
Regulatory peptides122(2)99-107(2004 Oct)
Depreitere J,Wang Z,Liang F,Coen E,Nouwen EJ

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

Cellular expression and specific intragranular localization of chromogranin A, chromogranin B, and synaptophysin during ontogeny of pancreatic islet cells: an ultrastructural study.
Pancreas27(1)38-46(2003 Jul)
Lukinius A,Stridsberg M,Wilander E

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CHGB cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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