SMG1 cDNA ORF clone, Sus scrofa(pig)

The following SMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe245762 XM_021086547.1
Latest version!
Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OSe245763 XM_021086548.1
Latest version!
Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OSe245764 XM_021086550.1
Latest version!
Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OSe245764 XM_021086549.1
Latest version!
Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OSe245765 XM_021086551.1
Latest version!
Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OSe245766 XM_021086552.1
Latest version!
Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OSe46686 XM_005658617.2
Latest version!
Sus scrofa SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe245762
    Clone ID Related Accession (Same CDS sequence) XM_021086547.1
    Accession Version XM_021086547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product serine/threonine-protein kinase SMG1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CACCAAGTAC 
    CCGCGGAGCT GGAATGACTG GCAACCCAGA ACTGATAGTG CATCAGCCGA CCCAGATAAT
    TTAAAATATT CTTCATCCAG AGATAGGGGT GGGTCTTCCT CTTATGGACT GCAACCTTCA
    AATTCAGCTG TGGTGTCTCG GCAAAGGCAC GATGATACCA GAATCCACGC TGACATACAA
    AATGACGAAA AGGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG
    AAGTCGTTGG GGCAAGAGCT GAGGGTTAAC AATGTGACCA GCCCTGAGTT CACCAGTATT
    CAGCATGGCA GTCGTGCGTT AGCCATCAAA GACATGAGGA AATCACAGGA GCGATCGATG
    TCTTATTCTG ATGAGTCTCG ACTGTCGAAT CTTCTTCGGA GGATCACCCG GGAAGACGAC
    AGAGACCGAA GACTGGCTAC TGTAAAGCAG TTGAAAGAGT TTATTCAGCA ACCAGAAAAT
    AAGCTGGTAC TAGTTAAACA ATTGGATAAT ATCTTGGCTG CTGTACATGA TGTGCTTAAT
    GAAAGTAGCA AATTGCTTCA GGAGTTGAGA CAGGAGGGAG CTTGCTGTCT TGGCCTTCTT
    TGTGCTTCTC TGAGCTATGA GGCCGAGAAG ATCTTCAAAT GGATTTTTAG CAAATTTAGC
    TCATCTGCAA AAGATGAAGT GAAACTTCTC TACTTATGTG CCACCTACAA AGCACTAGAG
    ACTGTAGGAG AAAAGAAAGC CTTTTCATCT GTAATGCAGC TTGTAATGAC CAGCCTGCAG
    TCCATTCTTG AAAATGTGGA TACACCAGAA TTACTTTGCA AATGTGTTAA GTGCATTCTT
    TTGGTAGCTC GATGTTACCC TCATATTTTC AGCACTAATT TTAGGGATAC AGTTGATATA
    TTAGTCGGAT GGCATATAGA TCATACTCAG AAGCCTTCCC TCACACAGCA GGTGTCTGGG
    TGGTTGCAGA GTTTGGAGCC ATTCTGGGTA GCTGATCTTG CATTTTCTAC CACTCTTCTT
    GGTCAGTTTC TAGAGGACAT GGAGGCATAT GCTGAGGACC TCAGCCATGT GGCCTCCGGG
    GAGTCAGTGG ATGAAGACGT TCCTCCTCCG TCAGTGTCCT TACCAAAGCT GGCAGCTCTC
    CTCCGGGTAT TCAGTACTGT GGTGAGAAGC ATTGGGGAAC GTTTCAGCCC AATTCGGGGT
    CCTCCAATTA CAGAGGCATA TGTAACAGAT GTTCTGTACA GAGTAATGAG ATGTGTGACG
    GCTGCAAACC AGGTGTTTTT TTCTGAGGCT GTGTTGACAG CTGCTAATGA GTGTGTTGGT
    GTTTTGCTCG GCAGCTTGGA TCCTAGCATG ACTATACATT GTGACATGGT CATTACATAT
    GGATTAGATC AGCTGGAGAA TTGCCAGACT TGTGGTACAG ATTATATCAT CTCAGTCTTG
    AATTTACTCA CATTGATTGT TGAACAGATA AATACAAAAT TGCCTTCATC ATTTGTAGAA
    AAACTATTTA TACCATCATC TAAACTACTA TTTTTGCGTT ATCATAAAGA AAAAGAGGTT
    GTTGCTGTAG CCCATGCTGT TTATCAAGCA GTGCTCAGCC TGAAGAATAT TCCTGTTTTG
    GAGACTGCCT ATAAATTAAT TTTGGGAGAA ATGACTTGTG CCCTAAACAA CCTGCTACAT
    AGTCTACAAC TTCCCGATGC CTGTTCTGAA ATAAAACATG AGGCTTTTAA GAATCATTTA
    TTCAACGTAG ACAATGCAAA ATTTGTAGTT ATATTTGACC TCAGTGCTCT GACTACAATT
    GGGAATGCTA AAAATTCACT AATTGGGATG TGGGCGCTGT CTCCAACCGT CTTTGCACTC
    CTGAGTAAGA ATCTGATGAT TGTGCATGGT GACCTGGCCG TTCACTTCCC TGCCATTCAG
    TATGCAGTGC TCTACACGTT GTATTCACAT TGTACTAGGC ATGACCACTT TATATCTAGT
    AGTCTGAGTT CTTCGTCCCC ATCTTTGTTT GATGGAGCCG TGATTAGTAC TGTAACCACA
    GCCACCAAAA AACATTTCTC AATTATATTA AATCTCTTGG GAATATTACT AAAGAAGGAT
    AATCTTAACC AGGACACAAG GAAACTGTTA ATGACCTGGG CTTTGGAAGT GGCTGTTTTA
    ATGAAGAAGT CTGAAACATA CGCACCTTTA TTCTCTCTGC CATCTTTCCA TAAGTTTTCC
    AAAGGCCTTT TAGCCAACAC TCTTGTTGAA GATGTGAATA TCTGTCTGCA AGCCTGCAGC
    AGTCTACATG CTTTGTCCTC GTCTTTACCG GATGATCTTT TACAGAGGTG TGTTGATGTT
    TGCCGTGTTC AACTAGTCCA TAGTGGAACT CGTATTCGCC AGGCATTTGG AAAGCTATTG
    AAGTCAATTC CTTTAGATGT TGTCTTGAGC AATAACAATC ACACAGAAAT TCAAGAAATT
    TCTTTAGCAT TAAGAAGTCA CATGAGTAAA GCACCAAGTA ATACATTCCA CCCACAAGAT
    TTCTCTGATG TTATTAGTTT TATTTTGTAT GGGAACTCTC ATAGAACGGG GAAGGACAAT
    TGGTTAGAAA GATTGTTCTA CAGCTGCCAG AGACTGGATA AACGTGACCA ATCAACAATT
    CCCCGCAATC TTCTAAAGAC AGATGCTGTC CTTTGGCAGT GGGCTATTTG GGAAGCAGCA
    CAGTTCACTG TCCTTTCTAA GTTGAGAACT CCACTGGGCA GAGCTCAGGA TACCTTCCAG
    ACAATTGAAG GTATCATCAG AAGTCTTGCA GCTCACACCT TAAACCCTGA TCAGGATGTT
    AGTCAGTGGA CAACTGCAGA CAATGATGAA GGCCACAGTA GCAACCAGCT TAGACTTGTG
    CTTCTTCTGC AGTATCTGGA AAACCTGGAG AAGTTAATGT ATAATGCATA TGAGGGATGT
    GCCAATGCGT TGACATCACC TCCCAAGGTC ATTAGGACTT TTTTCTATAC CAACCGCCAG
    ACTTGTCAAG ACTGGCTGAC CCGAATCCGA CTCTCCATCA TGAGGGTTGG ATTGTTAGCC
    GGCCAGCCTG CTGTGACAGT GAGACATGGC TTTGATTTGC TTACGGAAAT GAAGACAAAT
    CCATCTCAGG GGAATGAATT GGAAGTAACT ATCATGATGG TGGTAGAAGC ACTGTGTGAA
    CTTCATTGTC CAGAAGCTAT ACAAGGAATT GCTGTCTGGT CATCTTCCAT CGTTGGAAAA
    AATCTTCTCT GGATTAATTC AGTGGCCCAA CAGGCTGAAG GGAGGTTTGA AAAGGCCTCC
    GTGGAGTACC AGGAGCACCT GTGTGCCATG ACAGGTGTGG ATTGCTGCAT CTCCAGCTTT
    GACAAGTCTG TTCTCACATT GGCTAATACT GGGCGTAACA GTGCCAGCCC CAAACATTCT
    TTGAACGGTG AATCCAGAAA AACTGTGCTG TCCAAACCAA CTGACTCTTC TCCTGAGGTT
    ATAAATTATT TGGGAAATAA AGCATGTGAG TGCTACATTG CAATTGCCGA TTGGACTGCT
    GTACAGGAAT GGCAGAATGC TATCCATGAC TTGAAAAAGA GTACCAGCAG TACTTCTCTC
    AATCTAAAAG CTGATTTCAA CTACATAAAA TCACTAAGCA GTTTTGAATC TGGAGAATTT
    GTTGAATGTA CTGAGCAATT GGAATTGTTA CCAGGAGAAA ATATCAATCT ACTTGCTGGA
    GGATCAAAAG AAAAAATAGA TATGAAAAAA CTACTTCCTA ATATGTTAAG TCCAGATCCA
    AGAGAACTTC AGAAATCCAT CGAAGTTCAA TTGCTGAGAA GTTCTGTTTG TTTGGCAACT
    GCTTTAAACC ATATAGAACA GGATCAGAAG TGGCAGTCTA TAACTGAAAA CGTGGTGAAG
    TACTTGAAGC AAACCTCCCG CATAGCTATC GGGCCACTGA GACTTTCTAC TTTAACAGTT
    TCACAGTCTC TGCCAGTTCT AAGTACCTTG CAGCTGTATT GCTCTTCTGC TTTGGAGAAC
    ACAGTTTCCA ACAGACTTTC AACAGAGGAC TGTCTTATCC CACTCTTCAG TGAAGCTTTG
    CGTTCATGTA AACAGCATGA TGTCAGGCCA TGGATGCAGG CATTAAGATA CACCATGTAC
    CAGAGTCAGT TGTTGGAGAA AATAAAAGAA CAAACAGTTC CAATTAGAAG CCATCTCATG
    GAATTAGGTC TAACAGCAGC AAAATTTGCT AGAAAACGGG GGAATGTGTC ACTTGCAACA
    AGACTGCTGG CACAGTGCAG TGAGGTTCAG CTGGCAAAGA CCACGACTGC GCAGGATTTA
    GTCCAGCATT TTAAAAAGCT ATCAACTCAA GGTCAAGTGG ATGAAAAGTG GGGGCCTGAA
    CTTGACATTG AAAAAACCAA ATTGCTATAT ACAGCAGGCC AGTCAACACA CGCAATGGAG
    ATGCTGAGTT CTTGCGCCAT TTCGTTCTGC AAGTCTGCCA AAGCTGAGCA TGCTGTTGCC
    AAGTCCATTC TTACACTGGC CAAATGGATC CAGGCAGAAT GGAAAGAAAT ATCAGGGCAG
    CTGAAACAGG TTTACAGAGC TCAGCAGCAG CAAAACTTCA CAGGTCTTTC TACTTTATCT
    AAAAACATAC TCACTCTAAT TGAACTCCCA TCTGTTAATA CAGTGGAAGA AGAGTATCCT
    CGAATCGAGA GCGAATCTAC AGTACATATC GGAGTTGGAG AACCTGACTT CATTTTGGGA
    CAGTTGTATC ACCTATCTTC AGTACAGGCA CCTGAAGTAG CCAAATCTTG GGCAGCATTG
    GCTAGTTGGG CTTATAGATG GGGCAGAAAG GTCGTTGACA ATGCCAGTCA GGGAGAAGGT
    GTTCGTCTGT TGCCCAGAGA AAAATCTGAA GTTCAGAATC TGCTCCCAGA CACTATCACT
    GAAGAAGAAA AAGAGAGAAT ATATGGTATT CTTGGGCAGG CAGTGTGTCG GCCAGCAGGA
    ATTCAGGATG AAGATATAAC GCTTCAGATA ACAGAAAGTG AAGATAATGA GGAAGATGAT
    ATGGTTGATG TTATCTGGCG TCAGTTAATA TCGAGCTGCC CCTGGCTTTC AGAACTTGAT
    GAAAGTGCAA CAGAAGGCGT TATTAAAGTG TGGAGGAAAG TTGTAGATAG AATCTTCAGC
    CTGTACAAAC TGTCTTGCAG CGCCTATTTT ACTTTCCTTA AGCTCAATGC TGGTCAGATT
    CCTTTAGATG AAGATGACCC CAGGCTCCAT TTAAGTCACA GACCGGAGCA GAGCACGGAT
    GACGTGATTG TGATGGCCAC ATTGAGGCTG CTCAGACTGC TCGTGAAACA TGCCGGCGAG
    CTCAGGCAGT ATCTGGAACA TGGCTTGGAA ACAACACCCA CTGCTCCCTG GAGAGGAATT
    ATTCCACAAC TTTTCTCACG CTTAAACCAC CCTGAAGTGT ATGTGCGCCA AAGTATTTGT
    AACCTTCTCT GCCGTGTTGC TCAGGATTCC CCCCATCTCA TATTGTACCC TGCGATAGTG
    GGCACCATAT CGCTTAGCAG TGAGTCACAG GCTTCAGGAA ATAAGTTTTC CTCTGCAATT
    CCAACTTTAC TTGGCAATAT TCAAGGAGAA GAATTGCTGG TTTCTGAATG TGAGGGAGGA
    AGTCCTCCTG CTTCCCAAGA TAGCAATAAG GATGAACCGA AGAGTGGATT GAATGAAGAC
    CAAGCTATGA TGCAGGATTG CTACAGCAAG ATTGTAGATA AACTGTCCTC TGCAAACCCA
    ACTATGGTGT TACAGGTTCA GATGCTCGTG GCCGAGCTGC GCAGGGTCAC TGTTCTCTGG
    GATGAGCTCT GGTTGGGAGT TCTGTTACAG CAGCACATGT ATGTCCTGAG ACGAATTCAG
    CAGCTGGAAG ACGAAGTGAA GAGAGTCCAG AATAACAACA CCTTACGCAA AGAAGAGAAA
    ATTGCAATCA TGCGAGAGAA GCACACAGCT TTGATGAAGC CCATTGTGTT TGCTTTGGAG
    CACGTGAGGA GTATCACGGC GGCCCCTGCA GAAACGCCTC ATGAAAAGTG GTTTCAAGAT
    AACTATGGTG ATGCAATTGA AAATGCCCTA GAAAAACTGA AGACACCATC AAACCCTGCA
    AAGCCTGGAA GCAGTTGGAT TCCATTTAAA GAGATAATGC TAAGTTTGCA ACAGAGAGCA
    CAGAAACGTG CAAGCTACAT CTTGCGTCTT GAAGAAATCA GCCCGTGGCT GGCTGCCATG
    ACTAACACTG AAATTGCTCT TCCTGGAGAA GTCTCAGCCA GAGACACTGT CACAATCCAC
    AGTGTGGGCG GAACCATCAC AATCTTACCC ACCAAAACCA AGCCAAAGAA ACTTCTCTTT
    CTAGGATCGG ATGGGAAGAG CTATCCTTAT CTTTTCAAAG GATTGGAGGA CTTACATCTG
    GATGAGAGAA TAATGCAGTT TTTATCTATT GTAAATACTA TGTTTGCTAC AATCAATCGC
    CAGGAGACAC CCCGCTTCCA TGCGCGGCAC TATTCTGTAA CACCACTGGG AACTCGATCA
    GGACTGATCC AGTGGGTGGA TGGAGCCACA CCCTTATTTG GTCTTTACAA ACGATGGCAA
    CAACGGGAAG CTGCCTTACA AGCACAAAAG GCCCAAGATT CCTACCAGAC TCCTCAGAAT
    CCGGGAATTG TACCCCGTCC TAGTGAACTC TATTATAGTA AAATCGGTCC TGCTTTGAAA
    GCAGTGGGAC TTAGTCTGGA TGTGTCCCGA CGGGATTGGC CTCTTCATGT GATGAAGGCA
    GTGCTAGAAG AGTTGATGGA AGCCACGCCT CCAAATCTCC TTGCTAAAGA GCTGTGGTCA
    TCTTGTACCA CCCCTGATGA ATGGTGGAGA GTCACACAGT CTTACGCCAG ATCGACCGCG
    GTCATGTCTA TGGTCGGGTA CATAATTGGC CTTGGAGACA GACATCTGGA TAACGTCCTC
    ATAGATATGA CGACTGGAGA GGTTGTTCAC ATAGATTACA ATGTTTGCTT TGAAAAAGGT
    AAAAGCCTTA GAGTTCCTGA GAAAGTTCCT TTTCGAATGA CACAGAACAT TGAAACAGCA
    CTGGGTGTAA CTGGAGTAGA AGGTGTTTTT AGACTTTCAT GTGAGCAGGT TTTGCACATC
    ATGCGGCGTG GCAGAGAGAC TCTGCTGACA CTACTGGAGG CCTTTGTGTA CGACCCCCTG
    GTGGACTGGA CAGCTGGGGG CGAGGCTGGG TTTGCTGGTG CCGTCTACGG TGGGGGAGGC
    CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAAA GAGAAATCAC CCGCAGCCTG
    TTTTCTTCGA GAGTAGCTGA AATTAAGGTG AACTGGTTTA AGAATAGGGA CGAGATGCTG
    GTTGTACTGC CCAAGCTGGA CAGCAGCTTA GATGAATACC TGAGCTTGCA AGAGCAACTG
    ACAGATGTGG AAAAACTGCA GGGCAAACTT CTGGAAGAAA TAGAGTTTCT AGAAGGAGCT
    GAAGGAGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAACA CACTCAGCTA
    CAGACTCAGC AGAGAGCTGT TCAGGAGGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAG
    TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT GGCAAGTTTG
    CTTCAGGAGA TAAGCACACA GATGGACCTA GGTCCTCCAA GCTATGTGCC AGCAACAGCC
    TTTCTTCAGA ACGCTGGCCA GGCCCACTTG ATTAGCCAGT GTGAGCAGTT AGAGGGGGAA
    GTTGGCGCCC TCCTGCAGCA GAGGCGCTCG GTGCTGCGAG GCTGTCTGGA GCAGCTGCAT
    CACTATGCAA CTGTAGCTCT GCAATATCCA AAGGCCATAT TTCAGAAACA TCGAATTGAG
    CAGTGGAAGA CCTGGATGGA AGAACTTATC TGTAACACCA CTGTAGAGCG CTGTCAGGAA
    CTTTACAGGA AATATGAAAT GCAGTATGCA CCCCAGCCGC CCCCAACAGT GTGTCAGTTC
    ATCACTGCCA CTGAGATGAC CCTGCAGCGG TACGCAGCAG ATATAAACAG TAGGCTTATT
    AGACAGGTGG AACGATTGAA ACAAGAAGCC GTCACCGTAC CTGTTTGTGA AGATCAGCTG
    AAAGAAATTG AACGTTGCAT CAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG
    AGTCTAGCAA GTGTTATTAT CTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG
    GAAGGGGCAG CTTCGAGTGC TGGAGAGCAG TTGGTGGATC TGACTTCTCG GGATGGAGCC
    TGGTTCTTGG AGGAGCTCTG CAGTATGAGT GGAAATGTCA CTTGCCTTGT TCAGTTACTG
    AAGCAGTGTC ATCTGGTGCC ACAGGACTTA GACATCCCTA ACCCCATGGA GGCGTCTGAG
    GCAGTTCACT TAGCTAATGG AGTATACACC TCACTTCAGG AATTAAATTC AAATTTCCGG
    CAAATCATAT TTCCAGAAGC ACTGAGATGT TTAATGAAAG GCGAATACAC ATTAGAAAGT
    ATGCTTCATG AACTGGACAG CCTTATTGAG CAGACCACTG ATGGCGTTCC TCTGCAGACT
    CTAGTGGAAT CTCTTCAAGC CTATTTAAGA AACGCAGCCA TGGGACTTGA AGAAGAAACA
    CATGCCCATT ACATCGATGT TGCCAGAATA CTTCATGCTC AATATGGTGA GTTAATCCAA
    CCAAGAAATG GTTCAGTTGA TGAAACACCA AAAATGTCAG CTGGCCAGAT GCTTTTGGTA
    GCCTTTGATG GCATGTTTGC CCAAGTTGAG ACTGCTTTTG GCTTATTAGT TGAAAAGCTA
    AACAAGATGG AAATTCCTAT CGCTTGGAGA AAGATTGACA TTATAAGGGA AGCCAGGAGC
    ACTCAAGTCA ACTTTTTTGA TGATGATAAT CACCGACAGG TGCTGGAAGA GATTTTCTTT
    CTAAAACGAC TGCAGACAAT TAAGGAGTTC TTCAGACTCT GCGGTACCTT TTCTAAAACT
    TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTAA ATGGGCCTGT ACCGATTGTC
    AACGTGAAAA CTCTTTTTAG GAACTCTTGT TTCAGTGAAG ACCAGATGGC CAAACCTATA
    AAGGCCTTCA CAGCTGACTT CGTGCGGCAG CTCCTGATTG GACTCCCGAA CCAAGCCCTG
    GGCCTCACGC TGTGCAGCTT CATCAGTGCC CTAGGAGTGG ACATCATTGC TCAAGTGGAG
    GCCAAGGATT TTGGTGCTGA AAGCAAGGTT TCTGTTGATG ATCTGTGTAA GAAAGCAGTG
    GAGCACAACA TCCAGGTCGG GAAGTTCTCT CAGCTGGTCA TGAACAGGGC GACTGTGCTC
    GCCAGCTCCT ATGACACTGC CTGGAAGAAG CACGACTTGG TGCGCAGGCT GGAGACCAGT
    ATCTCCTCCT GTAAGACGAG CCTGCAGCGC GTTCAGCTGC ATATCGCCAT GTTTCAGTGG
    CAGCATGAAG ATCTCCTTAT CAACAGACCA CAAGCGATGT CGGTCACACC TCCCCCACGA
    TCTGCCATCC TAACCAGCAT GAAAAAGAAA CTCCATACTC TGAGCCAGAT TGAAACTTCA
    ATTGCAACAG TTCAGGAGAA ACTGGCAGCT CTTGAAGCAA GTATTGAACA GCGACTCAAG
    TGGGCAGGTG GTGCCAACCC TGCACTGGCA CCAGTACTAC AAGATTTTGA AGCAACAATA
    GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CCAGTCAGGT CACTTTCCTC
    TGCAGCAATA TTATTCATTT TGAAAGTTTA CGAACTAGAA CTGCGGAAGC CTTAAACCTG
    GATGCTGCAT TATTTGAGCT AATCAAACGG TGTCAGCAAA TGTGTTCATT TGCTTCACAG
    TTTAACAGTT CAGTCTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACAAGCCTT
    GAACACCCTA TTGGCAGCTC TGAATGGCTT TTATCAGCAC ACAAACAGTT GACCCAGGAT
    ATGTCTACGC AGAGAGCAAT TCAGACGGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA
    ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CAGGTCATAA CCGGCAGCTT
    GGAGATGTCA AACATCTCCT GAAAGCAATG GCCAAGGATG AAGAAGCTGC TCTGGCAGAT
    GGCGAAGATG TTCCCTATGA GAACAGTGTG AGGCAGTTCT TGGGTGAATA TAAATCTTGG
    CAAGACAACA TTCAGACTGT GCTGTTTACA TTAGTCCAAG CCATGGGTCA GGTTCGAAGT
    CAGGAACATG TTGAAATGCT CCAGGAAATA ACTCCCACCT TGAAAGAACT GAAAACACAA
    AGTCAGAGTG TCTATAATAA TTTAGTGAGT TTTGCATCAC CACTAGTCAC CGATGCAACA
    AATGAATGTT CAAGTCCAAC GTCATCTGCT ACGTATCAGC CATCCTTTGC TGCAGCAGTC
    CGGAGCAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CACAGAATGC TAGAAAGCTG
    ATTCAGAAAA ATCTAGCTAC ATCAGCAGAT ACTCCACCAA GCACTGTTCC AGGAACTGGC
    AAGAGTGCTG CTTGTAGTCC TAAAAAGGCC GTCAGAGACC CTAAAACTGG GAAAGCTGTG
    CAAGAGAGAA ATTCATATGC AGTGAGCGTG TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC
    CGAGATGTTG ATCCGAATAG GAGGATGTCA GTTGCTGAAC AGGTTGACTA TGTCATTAAG
    GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020942206.1
    CDS360..11339
    Translation

    Target ORF information:

    RefSeq Version XM_021086547.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021086547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CACCAAGTAC 
    CCGCGGAGCT GGAATGACTG GCAACCCAGA ACTGATAGTG CATCAGCCGA CCCAGATAAT
    TTAAAATATT CTTCATCCAG AGATAGGGGT GGGTCTTCCT CTTATGGACT GCAACCTTCA
    AATTCAGCTG TGGTGTCTCG GCAAAGGCAC GATGATACCA GAATCCACGC TGACATACAA
    AATGACGAAA AGGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG
    AAGTCGTTGG GGCAAGAGCT GAGGGTTAAC AATGTGACCA GCCCTGAGTT CACCAGTATT
    CAGCATGGCA GTCGTGCGTT AGCCATCAAA GACATGAGGA AATCACAGGA GCGATCGATG
    TCTTATTCTG ATGAGTCTCG ACTGTCGAAT CTTCTTCGGA GGATCACCCG GGAAGACGAC
    AGAGACCGAA GACTGGCTAC TGTAAAGCAG TTGAAAGAGT TTATTCAGCA ACCAGAAAAT
    AAGCTGGTAC TAGTTAAACA ATTGGATAAT ATCTTGGCTG CTGTACATGA TGTGCTTAAT
    GAAAGTAGCA AATTGCTTCA GGAGTTGAGA CAGGAGGGAG CTTGCTGTCT TGGCCTTCTT
    TGTGCTTCTC TGAGCTATGA GGCCGAGAAG ATCTTCAAAT GGATTTTTAG CAAATTTAGC
    TCATCTGCAA AAGATGAAGT GAAACTTCTC TACTTATGTG CCACCTACAA AGCACTAGAG
    ACTGTAGGAG AAAAGAAAGC CTTTTCATCT GTAATGCAGC TTGTAATGAC CAGCCTGCAG
    TCCATTCTTG AAAATGTGGA TACACCAGAA TTACTTTGCA AATGTGTTAA GTGCATTCTT
    TTGGTAGCTC GATGTTACCC TCATATTTTC AGCACTAATT TTAGGGATAC AGTTGATATA
    TTAGTCGGAT GGCATATAGA TCATACTCAG AAGCCTTCCC TCACACAGCA GGTGTCTGGG
    TGGTTGCAGA GTTTGGAGCC ATTCTGGGTA GCTGATCTTG CATTTTCTAC CACTCTTCTT
    GGTCAGTTTC TAGAGGACAT GGAGGCATAT GCTGAGGACC TCAGCCATGT GGCCTCCGGG
    GAGTCAGTGG ATGAAGACGT TCCTCCTCCG TCAGTGTCCT TACCAAAGCT GGCAGCTCTC
    CTCCGGGTAT TCAGTACTGT GGTGAGAAGC ATTGGGGAAC GTTTCAGCCC AATTCGGGGT
    CCTCCAATTA CAGAGGCATA TGTAACAGAT GTTCTGTACA GAGTAATGAG ATGTGTGACG
    GCTGCAAACC AGGTGTTTTT TTCTGAGGCT GTGTTGACAG CTGCTAATGA GTGTGTTGGT
    GTTTTGCTCG GCAGCTTGGA TCCTAGCATG ACTATACATT GTGACATGGT CATTACATAT
    GGATTAGATC AGCTGGAGAA TTGCCAGACT TGTGGTACAG ATTATATCAT CTCAGTCTTG
    AATTTACTCA CATTGATTGT TGAACAGATA AATACAAAAT TGCCTTCATC ATTTGTAGAA
    AAACTATTTA TACCATCATC TAAACTACTA TTTTTGCGTT ATCATAAAGA AAAAGAGGTT
    GTTGCTGTAG CCCATGCTGT TTATCAAGCA GTGCTCAGCC TGAAGAATAT TCCTGTTTTG
    GAGACTGCCT ATAAATTAAT TTTGGGAGAA ATGACTTGTG CCCTAAACAA CCTGCTACAT
    AGTCTACAAC TTCCCGATGC CTGTTCTGAA ATAAAACATG AGGCTTTTAA GAATCATTTA
    TTCAACGTAG ACAATGCAAA ATTTGTAGTT ATATTTGACC TCAGTGCTCT GACTACAATT
    GGGAATGCTA AAAATTCACT AATTGGGATG TGGGCGCTGT CTCCAACCGT CTTTGCACTC
    CTGAGTAAGA ATCTGATGAT TGTGCATGGT GACCTGGCCG TTCACTTCCC TGCCATTCAG
    TATGCAGTGC TCTACACGTT GTATTCACAT TGTACTAGGC ATGACCACTT TATATCTAGT
    AGTCTGAGTT CTTCGTCCCC ATCTTTGTTT GATGGAGCCG TGATTAGTAC TGTAACCACA
    GCCACCAAAA AACATTTCTC AATTATATTA AATCTCTTGG GAATATTACT AAAGAAGGAT
    AATCTTAACC AGGACACAAG GAAACTGTTA ATGACCTGGG CTTTGGAAGT GGCTGTTTTA
    ATGAAGAAGT CTGAAACATA CGCACCTTTA TTCTCTCTGC CATCTTTCCA TAAGTTTTCC
    AAAGGCCTTT TAGCCAACAC TCTTGTTGAA GATGTGAATA TCTGTCTGCA AGCCTGCAGC
    AGTCTACATG CTTTGTCCTC GTCTTTACCG GATGATCTTT TACAGAGGTG TGTTGATGTT
    TGCCGTGTTC AACTAGTCCA TAGTGGAACT CGTATTCGCC AGGCATTTGG AAAGCTATTG
    AAGTCAATTC CTTTAGATGT TGTCTTGAGC AATAACAATC ACACAGAAAT TCAAGAAATT
    TCTTTAGCAT TAAGAAGTCA CATGAGTAAA GCACCAAGTA ATACATTCCA CCCACAAGAT
    TTCTCTGATG TTATTAGTTT TATTTTGTAT GGGAACTCTC ATAGAACGGG GAAGGACAAT
    TGGTTAGAAA GATTGTTCTA CAGCTGCCAG AGACTGGATA AACGTGACCA ATCAACAATT
    CCCCGCAATC TTCTAAAGAC AGATGCTGTC CTTTGGCAGT GGGCTATTTG GGAAGCAGCA
    CAGTTCACTG TCCTTTCTAA GTTGAGAACT CCACTGGGCA GAGCTCAGGA TACCTTCCAG
    ACAATTGAAG GTATCATCAG AAGTCTTGCA GCTCACACCT TAAACCCTGA TCAGGATGTT
    AGTCAGTGGA CAACTGCAGA CAATGATGAA GGCCACAGTA GCAACCAGCT TAGACTTGTG
    CTTCTTCTGC AGTATCTGGA AAACCTGGAG AAGTTAATGT ATAATGCATA TGAGGGATGT
    GCCAATGCGT TGACATCACC TCCCAAGGTC ATTAGGACTT TTTTCTATAC CAACCGCCAG
    ACTTGTCAAG ACTGGCTGAC CCGAATCCGA CTCTCCATCA TGAGGGTTGG ATTGTTAGCC
    GGCCAGCCTG CTGTGACAGT GAGACATGGC TTTGATTTGC TTACGGAAAT GAAGACAAAT
    CCATCTCAGG GGAATGAATT GGAAGTAACT ATCATGATGG TGGTAGAAGC ACTGTGTGAA
    CTTCATTGTC CAGAAGCTAT ACAAGGAATT GCTGTCTGGT CATCTTCCAT CGTTGGAAAA
    AATCTTCTCT GGATTAATTC AGTGGCCCAA CAGGCTGAAG GGAGGTTTGA AAAGGCCTCC
    GTGGAGTACC AGGAGCACCT GTGTGCCATG ACAGGTGTGG ATTGCTGCAT CTCCAGCTTT
    GACAAGTCTG TTCTCACATT GGCTAATACT GGGCGTAACA GTGCCAGCCC CAAACATTCT
    TTGAACGGTG AATCCAGAAA AACTGTGCTG TCCAAACCAA CTGACTCTTC TCCTGAGGTT
    ATAAATTATT TGGGAAATAA AGCATGTGAG TGCTACATTG CAATTGCCGA TTGGACTGCT
    GTACAGGAAT GGCAGAATGC TATCCATGAC TTGAAAAAGA GTACCAGCAG TACTTCTCTC
    AATCTAAAAG CTGATTTCAA CTACATAAAA TCACTAAGCA GTTTTGAATC TGGAGAATTT
    GTTGAATGTA CTGAGCAATT GGAATTGTTA CCAGGAGAAA ATATCAATCT ACTTGCTGGA
    GGATCAAAAG AAAAAATAGA TATGAAAAAA CTACTTCCTA ATATGTTAAG TCCAGATCCA
    AGAGAACTTC AGAAATCCAT CGAAGTTCAA TTGCTGAGAA GTTCTGTTTG TTTGGCAACT
    GCTTTAAACC ATATAGAACA GGATCAGAAG TGGCAGTCTA TAACTGAAAA CGTGGTGAAG
    TACTTGAAGC AAACCTCCCG CATAGCTATC GGGCCACTGA GACTTTCTAC TTTAACAGTT
    TCACAGTCTC TGCCAGTTCT AAGTACCTTG CAGCTGTATT GCTCTTCTGC TTTGGAGAAC
    ACAGTTTCCA ACAGACTTTC AACAGAGGAC TGTCTTATCC CACTCTTCAG TGAAGCTTTG
    CGTTCATGTA AACAGCATGA TGTCAGGCCA TGGATGCAGG CATTAAGATA CACCATGTAC
    CAGAGTCAGT TGTTGGAGAA AATAAAAGAA CAAACAGTTC CAATTAGAAG CCATCTCATG
    GAATTAGGTC TAACAGCAGC AAAATTTGCT AGAAAACGGG GGAATGTGTC ACTTGCAACA
    AGACTGCTGG CACAGTGCAG TGAGGTTCAG CTGGCAAAGA CCACGACTGC GCAGGATTTA
    GTCCAGCATT TTAAAAAGCT ATCAACTCAA GGTCAAGTGG ATGAAAAGTG GGGGCCTGAA
    CTTGACATTG AAAAAACCAA ATTGCTATAT ACAGCAGGCC AGTCAACACA CGCAATGGAG
    ATGCTGAGTT CTTGCGCCAT TTCGTTCTGC AAGTCTGCCA AAGCTGAGCA TGCTGTTGCC
    AAGTCCATTC TTACACTGGC CAAATGGATC CAGGCAGAAT GGAAAGAAAT ATCAGGGCAG
    CTGAAACAGG TTTACAGAGC TCAGCAGCAG CAAAACTTCA CAGGTCTTTC TACTTTATCT
    AAAAACATAC TCACTCTAAT TGAACTCCCA TCTGTTAATA CAGTGGAAGA AGAGTATCCT
    CGAATCGAGA GCGAATCTAC AGTACATATC GGAGTTGGAG AACCTGACTT CATTTTGGGA
    CAGTTGTATC ACCTATCTTC AGTACAGGCA CCTGAAGTAG CCAAATCTTG GGCAGCATTG
    GCTAGTTGGG CTTATAGATG GGGCAGAAAG GTCGTTGACA ATGCCAGTCA GGGAGAAGGT
    GTTCGTCTGT TGCCCAGAGA AAAATCTGAA GTTCAGAATC TGCTCCCAGA CACTATCACT
    GAAGAAGAAA AAGAGAGAAT ATATGGTATT CTTGGGCAGG CAGTGTGTCG GCCAGCAGGA
    ATTCAGGATG AAGATATAAC GCTTCAGATA ACAGAAAGTG AAGATAATGA GGAAGATGAT
    ATGGTTGATG TTATCTGGCG TCAGTTAATA TCGAGCTGCC CCTGGCTTTC AGAACTTGAT
    GAAAGTGCAA CAGAAGGCGT TATTAAAGTG TGGAGGAAAG TTGTAGATAG AATCTTCAGC
    CTGTACAAAC TGTCTTGCAG CGCCTATTTT ACTTTCCTTA AGCTCAATGC TGGTCAGATT
    CCTTTAGATG AAGATGACCC CAGGCTCCAT TTAAGTCACA GACCGGAGCA GAGCACGGAT
    GACGTGATTG TGATGGCCAC ATTGAGGCTG CTCAGACTGC TCGTGAAACA TGCCGGCGAG
    CTCAGGCAGT ATCTGGAACA TGGCTTGGAA ACAACACCCA CTGCTCCCTG GAGAGGAATT
    ATTCCACAAC TTTTCTCACG CTTAAACCAC CCTGAAGTGT ATGTGCGCCA AAGTATTTGT
    AACCTTCTCT GCCGTGTTGC TCAGGATTCC CCCCATCTCA TATTGTACCC TGCGATAGTG
    GGCACCATAT CGCTTAGCAG TGAGTCACAG GCTTCAGGAA ATAAGTTTTC CTCTGCAATT
    CCAACTTTAC TTGGCAATAT TCAAGGAGAA GAATTGCTGG TTTCTGAATG TGAGGGAGGA
    AGTCCTCCTG CTTCCCAAGA TAGCAATAAG GATGAACCGA AGAGTGGATT GAATGAAGAC
    CAAGCTATGA TGCAGGATTG CTACAGCAAG ATTGTAGATA AACTGTCCTC TGCAAACCCA
    ACTATGGTGT TACAGGTTCA GATGCTCGTG GCCGAGCTGC GCAGGGTCAC TGTTCTCTGG
    GATGAGCTCT GGTTGGGAGT TCTGTTACAG CAGCACATGT ATGTCCTGAG ACGAATTCAG
    CAGCTGGAAG ACGAAGTGAA GAGAGTCCAG AATAACAACA CCTTACGCAA AGAAGAGAAA
    ATTGCAATCA TGCGAGAGAA GCACACAGCT TTGATGAAGC CCATTGTGTT TGCTTTGGAG
    CACGTGAGGA GTATCACGGC GGCCCCTGCA GAAACGCCTC ATGAAAAGTG GTTTCAAGAT
    AACTATGGTG ATGCAATTGA AAATGCCCTA GAAAAACTGA AGACACCATC AAACCCTGCA
    AAGCCTGGAA GCAGTTGGAT TCCATTTAAA GAGATAATGC TAAGTTTGCA ACAGAGAGCA
    CAGAAACGTG CAAGCTACAT CTTGCGTCTT GAAGAAATCA GCCCGTGGCT GGCTGCCATG
    ACTAACACTG AAATTGCTCT TCCTGGAGAA GTCTCAGCCA GAGACACTGT CACAATCCAC
    AGTGTGGGCG GAACCATCAC AATCTTACCC ACCAAAACCA AGCCAAAGAA ACTTCTCTTT
    CTAGGATCGG ATGGGAAGAG CTATCCTTAT CTTTTCAAAG GATTGGAGGA CTTACATCTG
    GATGAGAGAA TAATGCAGTT TTTATCTATT GTAAATACTA TGTTTGCTAC AATCAATCGC
    CAGGAGACAC CCCGCTTCCA TGCGCGGCAC TATTCTGTAA CACCACTGGG AACTCGATCA
    GGACTGATCC AGTGGGTGGA TGGAGCCACA CCCTTATTTG GTCTTTACAA ACGATGGCAA
    CAACGGGAAG CTGCCTTACA AGCACAAAAG GCCCAAGATT CCTACCAGAC TCCTCAGAAT
    CCGGGAATTG TACCCCGTCC TAGTGAACTC TATTATAGTA AAATCGGTCC TGCTTTGAAA
    GCAGTGGGAC TTAGTCTGGA TGTGTCCCGA CGGGATTGGC CTCTTCATGT GATGAAGGCA
    GTGCTAGAAG AGTTGATGGA AGCCACGCCT CCAAATCTCC TTGCTAAAGA GCTGTGGTCA
    TCTTGTACCA CCCCTGATGA ATGGTGGAGA GTCACACAGT CTTACGCCAG ATCGACCGCG
    GTCATGTCTA TGGTCGGGTA CATAATTGGC CTTGGAGACA GACATCTGGA TAACGTCCTC
    ATAGATATGA CGACTGGAGA GGTTGTTCAC ATAGATTACA ATGTTTGCTT TGAAAAAGGT
    AAAAGCCTTA GAGTTCCTGA GAAAGTTCCT TTTCGAATGA CACAGAACAT TGAAACAGCA
    CTGGGTGTAA CTGGAGTAGA AGGTGTTTTT AGACTTTCAT GTGAGCAGGT TTTGCACATC
    ATGCGGCGTG GCAGAGAGAC TCTGCTGACA CTACTGGAGG CCTTTGTGTA CGACCCCCTG
    GTGGACTGGA CAGCTGGGGG CGAGGCTGGG TTTGCTGGTG CCGTCTACGG TGGGGGAGGC
    CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAAA GAGAAATCAC CCGCAGCCTG
    TTTTCTTCGA GAGTAGCTGA AATTAAGGTG AACTGGTTTA AGAATAGGGA CGAGATGCTG
    GTTGTACTGC CCAAGCTGGA CAGCAGCTTA GATGAATACC TGAGCTTGCA AGAGCAACTG
    ACAGATGTGG AAAAACTGCA GGGCAAACTT CTGGAAGAAA TAGAGTTTCT AGAAGGAGCT
    GAAGGAGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAACA CACTCAGCTA
    CAGACTCAGC AGAGAGCTGT TCAGGAGGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAG
    TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT GGCAAGTTTG
    CTTCAGGAGA TAAGCACACA GATGGACCTA GGTCCTCCAA GCTATGTGCC AGCAACAGCC
    TTTCTTCAGA ACGCTGGCCA GGCCCACTTG ATTAGCCAGT GTGAGCAGTT AGAGGGGGAA
    GTTGGCGCCC TCCTGCAGCA GAGGCGCTCG GTGCTGCGAG GCTGTCTGGA GCAGCTGCAT
    CACTATGCAA CTGTAGCTCT GCAATATCCA AAGGCCATAT TTCAGAAACA TCGAATTGAG
    CAGTGGAAGA CCTGGATGGA AGAACTTATC TGTAACACCA CTGTAGAGCG CTGTCAGGAA
    CTTTACAGGA AATATGAAAT GCAGTATGCA CCCCAGCCGC CCCCAACAGT GTGTCAGTTC
    ATCACTGCCA CTGAGATGAC CCTGCAGCGG TACGCAGCAG ATATAAACAG TAGGCTTATT
    AGACAGGTGG AACGATTGAA ACAAGAAGCC GTCACCGTAC CTGTTTGTGA AGATCAGCTG
    AAAGAAATTG AACGTTGCAT CAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG
    AGTCTAGCAA GTGTTATTAT CTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG
    GAAGGGGCAG CTTCGAGTGC TGGAGAGCAG TTGGTGGATC TGACTTCTCG GGATGGAGCC
    TGGTTCTTGG AGGAGCTCTG CAGTATGAGT GGAAATGTCA CTTGCCTTGT TCAGTTACTG
    AAGCAGTGTC ATCTGGTGCC ACAGGACTTA GACATCCCTA ACCCCATGGA GGCGTCTGAG
    GCAGTTCACT TAGCTAATGG AGTATACACC TCACTTCAGG AATTAAATTC AAATTTCCGG
    CAAATCATAT TTCCAGAAGC ACTGAGATGT TTAATGAAAG GCGAATACAC ATTAGAAAGT
    ATGCTTCATG AACTGGACAG CCTTATTGAG CAGACCACTG ATGGCGTTCC TCTGCAGACT
    CTAGTGGAAT CTCTTCAAGC CTATTTAAGA AACGCAGCCA TGGGACTTGA AGAAGAAACA
    CATGCCCATT ACATCGATGT TGCCAGAATA CTTCATGCTC AATATGGTGA GTTAATCCAA
    CCAAGAAATG GTTCAGTTGA TGAAACACCA AAAATGTCAG CTGGCCAGAT GCTTTTGGTA
    GCCTTTGATG GCATGTTTGC CCAAGTTGAG ACTGCTTTTG GCTTATTAGT TGAAAAGCTA
    AACAAGATGG AAATTCCTAT CGCTTGGAGA AAGATTGACA TTATAAGGGA AGCCAGGAGC
    ACTCAAGTCA ACTTTTTTGA TGATGATAAT CACCGACAGG TGCTGGAAGA GATTTTCTTT
    CTAAAACGAC TGCAGACAAT TAAGGAGTTC TTCAGACTCT GCGGTACCTT TTCTAAAACT
    TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTAA ATGGGCCTGT ACCGATTGTC
    AACGTGAAAA CTCTTTTTAG GAACTCTTGT TTCAGTGAAG ACCAGATGGC CAAACCTATA
    AAGGCCTTCA CAGCTGACTT CGTGCGGCAG CTCCTGATTG GACTCCCGAA CCAAGCCCTG
    GGCCTCACGC TGTGCAGCTT CATCAGTGCC CTAGGAGTGG ACATCATTGC TCAAGTGGAG
    GCCAAGGATT TTGGTGCTGA AAGCAAGGTT TCTGTTGATG ATCTGTGTAA GAAAGCAGTG
    GAGCACAACA TCCAGGTCGG GAAGTTCTCT CAGCTGGTCA TGAACAGGGC GACTGTGCTC
    GCCAGCTCCT ATGACACTGC CTGGAAGAAG CACGACTTGG TGCGCAGGCT GGAGACCAGT
    ATCTCCTCCT GTAAGACGAG CCTGCAGCGC GTTCAGCTGC ATATCGCCAT GTTTCAGTGG
    CAGCATGAAG ATCTCCTTAT CAACAGACCA CAAGCGATGT CGGTCACACC TCCCCCACGA
    TCTGCCATCC TAACCAGCAT GAAAAAGAAA CTCCATACTC TGAGCCAGAT TGAAACTTCA
    ATTGCAACAG TTCAGGAGAA ACTGGCAGCT CTTGAAGCAA GTATTGAACA GCGACTCAAG
    TGGGCAGGTG GTGCCAACCC TGCACTGGCA CCAGTACTAC AAGATTTTGA AGCAACAATA
    GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CCAGTCAGGT CACTTTCCTC
    TGCAGCAATA TTATTCATTT TGAAAGTTTA CGAACTAGAA CTGCGGAAGC CTTAAACCTG
    GATGCTGCAT TATTTGAGCT AATCAAACGG TGTCAGCAAA TGTGTTCATT TGCTTCACAG
    TTTAACAGTT CAGTCTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACAAGCCTT
    GAACACCCTA TTGGCAGCTC TGAATGGCTT TTATCAGCAC ACAAACAGTT GACCCAGGAT
    ATGTCTACGC AGAGAGCAAT TCAGACGGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA
    ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CAGGTCATAA CCGGCAGCTT
    GGAGATGTCA AACATCTCCT GAAAGCAATG GCCAAGGATG AAGAAGCTGC TCTGGCAGAT
    GGCGAAGATG TTCCCTATGA GAACAGTGTG AGGCAGTTCT TGGGTGAATA TAAATCTTGG
    CAAGACAACA TTCAGACTGT GCTGTTTACA TTAGTCCAAG CCATGGGTCA GGTTCGAAGT
    CAGGAACATG TTGAAATGCT CCAGGAAATA ACTCCCACCT TGAAAGAACT GAAAACACAA
    AGTCAGAGTG TCTATAATAA TTTAGTGAGT TTTGCATCAC CACTAGTCAC CGATGCAACA
    AATGAATGTT CAAGTCCAAC GTCATCTGCT ACGTATCAGC CATCCTTTGC TGCAGCAGTC
    CGGAGCAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CACAGAATGC TAGAAAGCTG
    ATTCAGAAAA ATCTAGCTAC ATCAGCAGAT ACTCCACCAA GCACTGTTCC AGGAACTGGC
    AAGAGTGCTG CTTGTAGTCC TAAAAAGGCC GTCAGAGACC CTAAAACTGG GAAAGCTGTG
    CAAGAGAGAA ATTCATATGC AGTGAGCGTG TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC
    CGAGATGTTG ATCCGAATAG GAGGATGTCA GTTGCTGAAC AGGTTGACTA TGTCATTAAG
    GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe245763
    Clone ID Related Accession (Same CDS sequence) XM_021086548.1
    Accession Version XM_021086548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product serine/threonine-protein kinase SMG1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CACCAAGTAC 
    CCGCGGAGCT GGAATGACTG GCAACCCAGA ACTGATAGTG CATCAGCCGA CCCAGATAAT
    TTAAAATATT CTTCATCCAG AGATAGGGGT GGGTCTTCCT CTTATGGACT GCAACCTTCA
    AATTCAGCTG TGGTGTCTCG GCAAAGGCAC GATGATACCA GAATCCACGC TGACATACAA
    AATGACGAAA AGGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG
    AAGTCGTTGG GGCAAGAGCT GAGGGTTAAC AATGTGACCA GCCCTGAGTT CACCAGTATT
    CAGCATGGCA GTCGTGCGTT AGCCATCAAA GACATGAGGA AATCACAGGA GCGATCGATG
    TCTTATTCTG ATGAGTCTCG ACTGTCGAAT CTTCTTCGGA GGATCACCCG GGAAGACGAC
    AGAGACCGAA GACTGGCTAC TGTAAAGCAG TTGAAAGAGT TTATTCAGCA ACCAGAAAAT
    AAGCTGGTAC TAGTTAAACA ATTGGATAAT ATCTTGGCTG CTGTACATGA TGTGCTTAAT
    GAAAGTAGCA AATTGCTTCA GGAGTTGAGA CAGGAGGGAG CTTGCTGTCT TGGCCTTCTT
    TGTGCTTCTC TGAGCTATGA GGCCGAGAAG ATCTTCAAAT GGATTTTTAG CAAATTTAGC
    TCATCTGCAA AAGATGAAGT GAAACTTCTC TACTTATGTG CCACCTACAA AGCACTAGAG
    ACTGTAGGAG AAAAGAAAGC CTTTTCATCT GTAATGCAGC TTGTAATGAC CAGCCTGCAG
    TCCATTCTTG AAAATGTGGA TACACCAGAA TTACTTTGCA AATGTGTTAA GTGCATTCTT
    TTGGTAGCTC GATGTTACCC TCATATTTTC AGCACTAATT TTAGGGATAC AGTTGATATA
    TTAGTCGGAT GGCATATAGA TCATACTCAG AAGCCTTCCC TCACACAGCA GGTGTCTGGG
    TGGTTGCAGA GTTTGGAGCC ATTCTGGGTA GCTGATCTTG CATTTTCTAC CACTCTTCTT
    GGTCAGTTTC TAGAGGACAT GGAGGCATAT GCTGAGGACC TCAGCCATGT GGCCTCCGGG
    GAGTCAGTGG ATGAAGACGT TCCTCCTCCG TCAGTGTCCT TACCAAAGCT GGCAGCTCTC
    CTCCGGGTAT TCAGTACTGT GGTGAGAAGC ATTGGGGAAC GTTTCAGCCC AATTCGGGGT
    CCTCCAATTA CAGAGGCATA TGTAACAGAT GTTCTGTACA GAGTAATGAG ATGTGTGACG
    GCTGCAAACC AGGTGTTTTT TTCTGAGGCT GTGTTGACAG CTGCTAATGA GTGTGTTGGT
    GTTTTGCTCG GCAGCTTGGA TCCTAGCATG ACTATACATT GTGACATGGT CATTACATAT
    GGATTAGATC AGCTGGAGAA TTGCCAGACT TGTGGTACAG ATTATATCAT CTCAGTCTTG
    AATTTACTCA CATTGATTGT TGAACAGATA AATACAAAAT TGCCTTCATC ATTTGTAGAA
    AAACTATTTA TACCATCATC TAAACTACTA TTTTTGCGTT ATCATAAAGA AAAAGAGGTT
    GTTGCTGTAG CCCATGCTGT TTATCAAGCA GTGCTCAGCC TGAAGAATAT TCCTGTTTTG
    GAGACTGCCT ATAAATTAAT TTTGGGAGAA ATGACTTGTG CCCTAAACAA CCTGCTACAT
    AGTCTACAAC TTCCCGATGC CTGTTCTGAA ATAAAACATG AGGCTTTTAA GAATCATTTA
    TTCAACGTAG ACAATGCAAA ATTTGTAGTT ATATTTGACC TCAGTGCTCT GACTACAATT
    GGGAATGCTA AAAATTCACT AATTGGGATG TGGGCGCTGT CTCCAACCGT CTTTGCACTC
    CTGAGTAAGA ATCTGATGAT TGTGCATGGT GACCTGGCCG TTCACTTCCC TGCCATTCAG
    TATGCAGTGC TCTACACGTT GTATTCACAT TGTACTAGGC ATGACCACTT TATATCTAGT
    AGTCTGAGTT CTTCGTCCCC ATCTTTGTTT GATGGAGCCG TGATTAGTAC TGTAACCACA
    GCCACCAAAA AACATTTCTC AATTATATTA AATCTCTTGG GAATATTACT AAAGAAGGAT
    AATCTTAACC AGGACACAAG GAAACTGTTA ATGACCTGGG CTTTGGAAGT GGCTGTTTTA
    ATGAAGAAGT CTGAAACATA CGCACCTTTA TTCTCTCTGC CATCTTTCCA TAAGTTTTCC
    AAAGGCCTTT TAGCCAACAC TCTTGTTGAA GATGTGAATA TCTGTCTGCA AGCCTGCAGC
    AGTCTACATG CTTTGTCCTC GTCTTTACCG GATGATCTTT TACAGAGGTG TGTTGATGTT
    TGCCGTGTTC AACTAGTCCA TAGTGGAACT CGTATTCGCC AGGCATTTGG AAAGCTATTG
    AAGTCAATTC CTTTAGATGT TGTCTTGAGC AATAACAATC ACACAGAAAT TCAAGAAATT
    TCTTTAGCAT TAAGAAGTCA CATGAGTAAA GCACCAAGTA ATACATTCCA CCCACAAGAT
    TTCTCTGATG TTATTAGTTT TATTTTGTAT GGGAACTCTC ATAGAACGGG GAAGGACAAT
    TGGTTAGAAA GATTGTTCTA CAGCTGCCAG AGACTGGATA AACGTGACCA ATCAACAATT
    CCCCGCAATC TTCTAAAGAC AGATGCTGTC CTTTGGCAGT GGGCTATTTG GGAAGCAGCA
    CAGTTCACTG TCCTTTCTAA GTTGAGAACT CCACTGGGCA GAGCTCAGGA TACCTTCCAG
    ACAATTGAAG GTATCATCAG AAGTCTTGCA GCTCACACCT TAAACCCTGA TCAGGATGTT
    AGTCAGTGGA CAACTGCAGA CAATGATGAA GGCCACAGTA GCAACCAGCT TAGACTTGTG
    CTTCTTCTGC AGTATCTGGA AAACCTGGAG AAGTTAATGT ATAATGCATA TGAGGGATGT
    GCCAATGCGT TGACATCACC TCCCAAGGTC ATTAGGACTT TTTTCTATAC CAACCGCCAG
    ACTTGTCAAG ACTGGCTGAC CCGAATCCGA CTCTCCATCA TGAGGGTTGG ATTGTTAGCC
    GGCCAGCCTG CTGTGACAGT GAGACATGGC TTTGATTTGC TTACGGAAAT GAAGACAAAT
    CCATCTCAGG GGAATGAATT GGAAGTAACT ATCATGATGG TGGTAGAAGC ACTGTGTGAA
    CTTCATTGTC CAGAAGCTAT ACAAGGAATT GCTGTCTGGT CATCTTCCAT CGTTGGAAAA
    AATCTTCTCT GGATTAATTC AGTGGCCCAA CAGGCTGAAG GGAGGTTTGA AAAGGCCTCC
    GTGGAGTACC AGGAGCACCT GTGTGCCATG ACAGGTGTGG ATTGCTGCAT CTCCAGCTTT
    GACAAGTCTG TTCTCACATT GGCTAATACT GGGCGTAACA GTGCCAGCCC CAAACATTCT
    TTGAACGGTG AATCCAGAAA AACTGTGCTG TCCAAACCAA CTGACTCTTC TCCTGAGGTT
    ATAAATTATT TGGGAAATAA AGCATGTGAG TGCTACATTG CAATTGCCGA TTGGACTGCT
    GTACAGGAAT GGCAGAATGC TATCCATGAC TTGAAAAAGA GTACCAGCAG TACTTCTCTC
    AATCTAAAAG CTGATTTCAA CTACATAAAA TCACTAAGCA GTTTTGAATC TGGAGAATTT
    GTTGAATGTA CTGAGCAATT GGAATTGTTA CCAGGAGAAA ATATCAATCT ACTTGCTGGA
    GGATCAAAAG AAAAAATAGA TATGAAAAAA CTACTTCCTA ATATGTTAAG TCCAGATCCA
    AGAGAACTTC AGAAATCCAT CGAAGTTCAA TTGCTGAGAA GTTCTGTTTG TTTGGCAACT
    GCTTTAAACC ATATAGAACA GGATCAGAAG TGGCAGTCTA TAACTGAAAA CGTGGTGAAG
    TACTTGAAGC AAACCTCCCG CATAGCTATC GGGCCACTGA GACTTTCTAC TTTAACAGTT
    TCACAGTCTC TGCCAGTTCT AAGTACCTTG CAGCTGTATT GCTCTTCTGC TTTGGAGAAC
    ACAGTTTCCA ACAGACTTTC AACAGAGGAC TGTCTTATCC CACTCTTCAG TGAAGCTTTG
    CGTTCATGTA AACAGCATGA TGTCAGGCCA TGGATGCAGG CATTAAGATA CACCATGTAC
    CAGAGTCAGT TGTTGGAGAA AATAAAAGAA CAAACAGTTC CAATTAGAAG CCATCTCATG
    GAATTAGGTC TAACAGCAGC AAAATTTGCT AGAAAACGGG GGAATGTGTC ACTTGCAACA
    AGACTGCTGG CACAGTGCAG TGAGGTTCAG CTGGCAAAGA CCACGACTGC GCAGGATTTA
    GTCCAGCATT TTAAAAAGCT ATCAACTCAA GGTCAAGTGG ATGAAAAGTG GGGGCCTGAA
    CTTGACATTG AAAAAACCAA ATTGCTATAT ACAGCAGGCC AGTCAACACA CGCAATGGAG
    ATGCTGAGTT CTTGCGCCAT TTCGTTCTGC AAGTCTGCCA AAGCTGAGCA TGCTGTTGCC
    AAGTCCATTC TTACACTGGC CAAATGGATC CAGGCAGAAT GGAAAGAAAT ATCAGGGCAG
    CTGAAACAGG TTTACAGAGC TCAGCAGCAG CAAAACTTCA CAGGTCTTTC TACTTTATCT
    AAAAACATAC TCACTCTAAT TGAACTCCCA TCTGTTAATA CAGTGGAAGA AGAGTATCCT
    CGAATCGAGA GCGAATCTAC AGTACATATC GGAGTTGGAG AACCTGACTT CATTTTGGGA
    CAGTTGTATC ACCTATCTTC AGTACAGGCA CCTGAAGTAG CCAAATCTTG GGCAGCATTG
    GCTAGTTGGG CTTATAGATG GGGCAGAAAG GTCGTTGACA ATGCCAGTCA GGGAGAAGGT
    GTTCGTCTGT TGCCCAGAGA AAAATCTGAA GTTCAGAATC TGCTCCCAGA CACTATCACT
    GAAGAAGAAA AAGAGAGAAT ATATGGTATT CTTGGGCAGG CAGTGTGTCG GCCAGCAGGA
    ATTCAGGATG AAGATATAAC GCTTCAGATA ACAGAAAGTG AAGATAATGA GGAAGATGAT
    ATGGTTGATG TTATCTGGCG TCAGTTAATA TCGAGCTGCC CCTGGCTTTC AGAACTTGAT
    GAAAGTGCAA CAGAAGGCGT TATTAAAGTG TGGAGGAAAG TTGTAGATAG AATCTTCAGC
    CTGTACAAAC TGTCTTGCAG CGCCTATTTT ACTTTCCTTA AGCTCAATGC TGGTCAGATT
    CCTTTAGATG AAGATGACCC CAGGCTCCAT TTAAGTCACA GACCGGAGCA GAGCACGGAT
    GACGTGATTG TGATGGCCAC ATTGAGGCTG CTCAGACTGC TCGTGAAACA TGCCGGCGAG
    CTCAGGCAGT ATCTGGAACA TGGCTTGGAA ACAACACCCA CTGCTCCCTG GAGAGGAATT
    ATTCCACAAC TTTTCTCACG CTTAAACCAC CCTGAAGTGT ATGTGCGCCA AAGTATTTGT
    AACCTTCTCT GCCGTGTTGC TCAGGATTCC CCCCATCTCA TATTGTACCC TGCGATAGTG
    GGCACCATAT CGCTTAGCAG TGAGTCACAG GCTTCAGGAA ATAAGTTTTC CTCTGCAATT
    CCAACTTTAC TTGGCAATAT TCAAGGAGAA GAATTGCTGG TTTCTGAATG TGAGGGAGGA
    AGTCCTCCTG CTTCCCAAGA TAGCAATAAG GATGAACCGA AGAGTGGATT GAATGAAGAC
    CAAGCTATGA TGCAGGATTG CTACAGCAAG ATTGTAGATA AACTGTCCTC TGCAAACCCA
    ACTATGGTGT TACAGGTTCA GATGCTCGTG GCCGAGCTGC GCAGGGTCAC TGTTCTCTGG
    GATGAGCTCT GGTTGGGAGT TCTGTTACAG CAGCACATGT ATGTCCTGAG ACGAATTCAG
    CAGCTGGAAG ACGAAGTGAA GAGAGTCCAG AATAACAACA CCTTACGCAA AGAAGAGAAA
    ATTGCAATCA TGCGAGAGAA GCACACAGCT TTGATGAAGC CCATTGTGTT TGCTTTGGAG
    CACGTGAGGA GTATCACGGC GGCCCCTGCA GAAACGCCTC ATGAAAAGTG GTTTCAAGAT
    AACTATGGTG ATGCAATTGA AAATGCCCTA GAAAAACTGA AGACACCATC AAACCCTGCA
    AAGCCTGGAA GCAGTTGGAT TCCATTTAAA GAGATAATGC TAAGTTTGCA ACAGAGAGCA
    CAGAAACGTG CAAGCTACAT CTTGCGTCTT GAAGAAATCA GCCCGTGGCT GGCTGCCATG
    ACTAACACTG AAATTGCTCT TCCTGGAGAA GTCTCAGCCA GAGACACTGT CACAATCCAC
    AGTGTGGGCG GAACCATCAC AATCTTACCC ACCAAAACCA AGCCAAAGAA ACTTCTCTTT
    CTAGGATCGG ATGGGAAGAG CTATCCTTAT CTTTTCAAAG GATTGGAGGA CTTACATCTG
    GATGAGAGAA TAATGCAGTT TTTATCTATT GTAAATACTA TGTTTGCTAC AATCAATCGC
    CAGGAGACAC CCCGCTTCCA TGCGCGGCAC TATTCTGTAA CACCACTGGG AACTCGATCA
    GGACTGATCC AGTGGGTGGA TGGAGCCACA CCCTTATTTG GTCTTTACAA ACGATGGCAA
    CAACGGGAAG CTGCCTTACA AGCACAAAAG GCCCAAGATT CCTACCAGAC TCCTCAGAAT
    CCGGGAATTG TACCCCGTCC TAGTGAACTC TATTATAGTA AAATCGGTCC TGCTTTGAAA
    GCAGTGGGAC TTAGTCTGGA TGTGTCCCGA CGGGATTGGC CTCTTCATGT GATGAAGGCA
    GTGCTAGAAG AGTTGATGGA AGCCACGCCT CCAAATCTCC TTGCTAAAGA GCTGTGGTCA
    TCTTGTACCA CCCCTGATGA ATGGTGGAGA GTCACACAGT CTTACGCCAG ATCGACCGCG
    GTCATGTCTA TGGTCGGGTA CATAATTGGC CTTGGAGACA GACATCTGGA TAACGTCCTC
    ATAGATATGA CGACTGGAGA GGTTGTTCAC ATAGATTACA ATGTTTGCTT TGAAAAAGGT
    AAAAGCCTTA GAGTTCCTGA GAAAGTTCCT TTTCGAATGA CACAGAACAT TGAAACAGCA
    CTGGGTGTAA CTGGAGTAGA AGGTGTTTTT AGACTTTCAT GTGAGCAGGT TTTGCACATC
    ATGCGGCGTG GCAGAGAGAC TCTGCTGACA CTACTGGAGG CCTTTGTGTA CGACCCCCTG
    GTGGACTGGA CAGCTGGGGG CGAGGCTGGG TTTGCTGGTG CCGTCTACGG TGGGGGAGGC
    CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAAA GAGAAATCAC CCGCAGCCTG
    TTTTCTTCGA GAGTAGCTGA AATTAAGGTG AACTGGTTTA AGAATAGGGA CGAGATGCTG
    GTTGTACTGC CCAAGCTGGA CAGCAGCTTA GATGAATACC TGAGCTTGCA AGAGCAACTG
    ACAGATGTGG AAAAACTGCA GGGCAAACTT CTGGAAGAAA TAGAGTTTCT AGAAGGAGCT
    GAAGGAGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAACA CACTCAGCTA
    CAGACTCAGC AGAGAGCTGT TCAGGAGGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAG
    TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT GGCAAGTTTG
    CTTCAGGAGA TAAGCACACA GATGGACCTA GGTCCTCCAA GCTATGTGCC AGCAACAGCC
    TTTCTTCAGA ACGCTGGCCA GGCCCACTTG ATTAGCCAGT GTGAGCAGTT AGAGGGGGAA
    GTTGGCGCCC TCCTGCAGCA GAGGCGCTCG GTGCTGCGAG GCTGTCTGGA GCAGCTGCAT
    CACTATGCAA CTGTAGCTCT GCAATATCCA AAGGCCATAT TTCAGAAACA TCGAATTGAG
    CAGTGGAAGA CCTGGATGGA AGAACTTATC TGTAACACCA CTGTAGAGCG CTGTCAGGAA
    CTTTACAGGA AATATGAAAT GCAGTATGCA CCCCAGCCGC CCCCAACAGT GTGTCAGTTC
    ATCACTGCCA CTGAGATGAC CCTGCAGCGG TACGCAGCAG ATATAAACAG TAGGCTTATT
    AGACAGGTGG AACGATTGAA ACAAGAAGCC GTCACCGTAC CTGTTTGTGA AGATCAGCTG
    AAAGAAATTG AACGTTGCAT CAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG
    AGTCTAGCAA GTGTTATTAT CTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG
    GAAGGGGCAG CTTCGAGTGC TGGAGAGCAG TTGGTGGATC TGACTTCTCG GGATGGAGCC
    TGGTTCTTGG AGGAGCTCTG CAGTATGAGT GGAAATGTCA CTTGCCTTGT TCAGTTACTG
    AAGCAGTGTC ATCTGGTGCC ACAGGACTTA GACATCCCTA ACCCCATGGA GGCGTCTGAG
    GCAGTTCACT TAGCTAATGG AGTATACACC TCACTTCAGG AATTAAATTC AAATTTCCGG
    CAAATCATAT TTCCAGAAGC ACTGAGATGT TTAATGAAAG GCGAATACAC ATTAGAAAGT
    ATGCTTCATG AACTGGACAG CCTTATTGAG CAGACCACTG ATGGCGTTCC TCTGCAGACT
    CTAGTGGAAT CTCTTCAAGC CTATTTAAGA AACGCAGCCA TGGGACTTGA AGAAGAAACA
    CATGCCCATT ACATCGATGT TGCCAGAATA CTTCATGCTC AATATGGTGA GTTAATCCAA
    CCAAGAAATG GTTCAGTTGA TGAAACACCA AAAATGTCAG CTGGCCAGAT GCTTTTGGTA
    GCCTTTGATG GCATGTTTGC CCAAGTTGAG ACTGCTTTTG GCTTATTAGT TGAAAAGCTA
    AACAAGATGG AAATTCCTAT CGCTTGGAGA AAGATTGACA TTATAAGGGA AGCCAGGAGC
    ACTCAAGTCA ACTTTTTTGA TGATGATAAT CACCGACAGG TGCTGGAAGA GATTTTCTTT
    CTAAAACGAC TGCAGACAAT TAAGGAGTTC TTCAGACTCT GCGGTACCTT TTCTAAAACT
    TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTAA ATGGGCCTGT ACCGATTGTC
    AACGTGAAAA CTCTTTTTAG GAACTCTTGT TTCAGTGAAG ACCAGATGGC CAAACCTATA
    AAGGCCTTCA CAGCTGACTT CGTGCGGCAG CTCCTGATTG GACTCCCGAA CCAAGCCCTG
    GGCCTCACGC TGTGCAGCTT CATCAGTGCC CTAGGAGTGG ACATCATTGC TCAAGTGGAG
    GCCAAGGATT TTGGTGCTGA AAGCAAGGTT TCTGTTGATG ATCTGTGTAA GAAAGCAGTG
    GAGCACAACA TCCAGGTCGG GAAGTTCTCT CAGCTGGTCA TGAACAGGGC GACTGTGCTC
    GCCAGCTCCT ATGACACTGC CTGGAAGAAG CACGACTTGG TGCGCAGGCT GGAGACCAGT
    ATCTCCTCCT GTAAGACGAG CCTGCAGCGC GTTCAGCTGC ATATCGCCAT GTTTCAGTGG
    CAGCATGAAG ATCTCCTTAT CAACAGACCA CAAGCGATGT CGGTCACACC TCCCCCACGA
    TCTGCCATCC TAACCAGCAT GAAAAAGAAA CTCCATACTC TGAGCCAGAT TGAAACTTCA
    ATTGCAACAG TTCAGGAGAA ACTGGCAGCT CTTGAAGCAA GTATTGAACA GCGACTCAAG
    TGGGCAGGTG GTGCCAACCC TGCACTGGCA CCAGTACTAC AAGATTTTGA AGCAACAATA
    GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CCAGTCAGGT CACTTTCCTC
    TGCAGCAATA TTATTCATTT TGAAAGTTTA CGAACTAGAA CTGCGGAAGC CTTAAACCTG
    GATGCTGCAT TATTTGAGCT AATCAAACGG TGTCAGCAAA TGTGTTCATT TGCTTCACAG
    TTTAACAGTT CAGTCTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACAAGCCTT
    GAACACCCTA TTGGCAGCTC TGAATGGCTT TTATCAGCAC ACAAACAGTT GACCCAGGAT
    ATGTCTACGC AGAGAGCAAT TCAGACGGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA
    ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CAGGTCATAA CCGGCAGCTT
    GGAGATGTCA AACATCTCCT GAAAGCAATG GCCAAGGATG AAGAAGCTGC TCTGGCAGAT
    GGCGAAGATG TTCCCTATGA GAACAGTGTG AGGCAGTTCT TGGGTGAATA TAAATCTTGG
    CAAGACAACA TTCAGACTGT GCTGTTTACA TTAGTCCAAG CCATGGGTCA GGTTCGAAGT
    CAGGAACATG TTGAAATGCT CCAGGAAATA ACTCCCACCT TGAAAGAACT GAAAACACAA
    AGTCAGAGTG TCTATAATAA TTTAGTGAGT TTTGCATCAC CACTAGTCAC CGATGCAACA
    AATGAATGTT CAAGTCCAAC GTCATCTGCT ACGTATCAGC CATCCTTTGC TGCAGTCCGG
    AGCAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCAC AGAATGCTAG AAAGCTGATT
    CAGAAAAATC TAGCTACATC AGCAGATACT CCACCAAGCA CTGTTCCAGG AACTGGCAAG
    AGTGCTGCTT GTAGTCCTAA AAAGGCCGTC AGAGACCCTA AAACTGGGAA AGCTGTGCAA
    GAGAGAAATT CATATGCAGT GAGCGTGTGG AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA
    GATGTTGATC CGAATAGGAG GATGTCAGTT GCTGAACAGG TTGACTATGT CATTAAGGAA
    GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020942207.1
    CDS360..11336
    Translation

    Target ORF information:

    RefSeq Version XM_021086548.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021086548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CACCAAGTAC 
    CCGCGGAGCT GGAATGACTG GCAACCCAGA ACTGATAGTG CATCAGCCGA CCCAGATAAT
    TTAAAATATT CTTCATCCAG AGATAGGGGT GGGTCTTCCT CTTATGGACT GCAACCTTCA
    AATTCAGCTG TGGTGTCTCG GCAAAGGCAC GATGATACCA GAATCCACGC TGACATACAA
    AATGACGAAA AGGGTGGCTA CAGTGTCAAT GGAGGATCTG GGGAAAATAC TTATGGTCGG
    AAGTCGTTGG GGCAAGAGCT GAGGGTTAAC AATGTGACCA GCCCTGAGTT CACCAGTATT
    CAGCATGGCA GTCGTGCGTT AGCCATCAAA GACATGAGGA AATCACAGGA GCGATCGATG
    TCTTATTCTG ATGAGTCTCG ACTGTCGAAT CTTCTTCGGA GGATCACCCG GGAAGACGAC
    AGAGACCGAA GACTGGCTAC TGTAAAGCAG TTGAAAGAGT TTATTCAGCA ACCAGAAAAT
    AAGCTGGTAC TAGTTAAACA ATTGGATAAT ATCTTGGCTG CTGTACATGA TGTGCTTAAT
    GAAAGTAGCA AATTGCTTCA GGAGTTGAGA CAGGAGGGAG CTTGCTGTCT TGGCCTTCTT
    TGTGCTTCTC TGAGCTATGA GGCCGAGAAG ATCTTCAAAT GGATTTTTAG CAAATTTAGC
    TCATCTGCAA AAGATGAAGT GAAACTTCTC TACTTATGTG CCACCTACAA AGCACTAGAG
    ACTGTAGGAG AAAAGAAAGC CTTTTCATCT GTAATGCAGC TTGTAATGAC CAGCCTGCAG
    TCCATTCTTG AAAATGTGGA TACACCAGAA TTACTTTGCA AATGTGTTAA GTGCATTCTT
    TTGGTAGCTC GATGTTACCC TCATATTTTC AGCACTAATT TTAGGGATAC AGTTGATATA
    TTAGTCGGAT GGCATATAGA TCATACTCAG AAGCCTTCCC TCACACAGCA GGTGTCTGGG
    TGGTTGCAGA GTTTGGAGCC ATTCTGGGTA GCTGATCTTG CATTTTCTAC CACTCTTCTT
    GGTCAGTTTC TAGAGGACAT GGAGGCATAT GCTGAGGACC TCAGCCATGT GGCCTCCGGG
    GAGTCAGTGG ATGAAGACGT TCCTCCTCCG TCAGTGTCCT TACCAAAGCT GGCAGCTCTC
    CTCCGGGTAT TCAGTACTGT GGTGAGAAGC ATTGGGGAAC GTTTCAGCCC AATTCGGGGT
    CCTCCAATTA CAGAGGCATA TGTAACAGAT GTTCTGTACA GAGTAATGAG ATGTGTGACG
    GCTGCAAACC AGGTGTTTTT TTCTGAGGCT GTGTTGACAG CTGCTAATGA GTGTGTTGGT
    GTTTTGCTCG GCAGCTTGGA TCCTAGCATG ACTATACATT GTGACATGGT CATTACATAT
    GGATTAGATC AGCTGGAGAA TTGCCAGACT TGTGGTACAG ATTATATCAT CTCAGTCTTG
    AATTTACTCA CATTGATTGT TGAACAGATA AATACAAAAT TGCCTTCATC ATTTGTAGAA
    AAACTATTTA TACCATCATC TAAACTACTA TTTTTGCGTT ATCATAAAGA AAAAGAGGTT
    GTTGCTGTAG CCCATGCTGT TTATCAAGCA GTGCTCAGCC TGAAGAATAT TCCTGTTTTG
    GAGACTGCCT ATAAATTAAT TTTGGGAGAA ATGACTTGTG CCCTAAACAA CCTGCTACAT
    AGTCTACAAC TTCCCGATGC CTGTTCTGAA ATAAAACATG AGGCTTTTAA GAATCATTTA
    TTCAACGTAG ACAATGCAAA ATTTGTAGTT ATATTTGACC TCAGTGCTCT GACTACAATT
    GGGAATGCTA AAAATTCACT AATTGGGATG TGGGCGCTGT CTCCAACCGT CTTTGCACTC
    CTGAGTAAGA ATCTGATGAT TGTGCATGGT GACCTGGCCG TTCACTTCCC TGCCATTCAG
    TATGCAGTGC TCTACACGTT GTATTCACAT TGTACTAGGC ATGACCACTT TATATCTAGT
    AGTCTGAGTT CTTCGTCCCC ATCTTTGTTT GATGGAGCCG TGATTAGTAC TGTAACCACA
    GCCACCAAAA AACATTTCTC AATTATATTA AATCTCTTGG GAATATTACT AAAGAAGGAT
    AATCTTAACC AGGACACAAG GAAACTGTTA ATGACCTGGG CTTTGGAAGT GGCTGTTTTA
    ATGAAGAAGT CTGAAACATA CGCACCTTTA TTCTCTCTGC CATCTTTCCA TAAGTTTTCC
    AAAGGCCTTT TAGCCAACAC TCTTGTTGAA GATGTGAATA TCTGTCTGCA AGCCTGCAGC
    AGTCTACATG CTTTGTCCTC GTCTTTACCG GATGATCTTT TACAGAGGTG TGTTGATGTT
    TGCCGTGTTC AACTAGTCCA TAGTGGAACT CGTATTCGCC AGGCATTTGG AAAGCTATTG
    AAGTCAATTC CTTTAGATGT TGTCTTGAGC AATAACAATC ACACAGAAAT TCAAGAAATT
    TCTTTAGCAT TAAGAAGTCA CATGAGTAAA GCACCAAGTA ATACATTCCA CCCACAAGAT
    TTCTCTGATG TTATTAGTTT TATTTTGTAT GGGAACTCTC ATAGAACGGG GAAGGACAAT
    TGGTTAGAAA GATTGTTCTA CAGCTGCCAG AGACTGGATA AACGTGACCA ATCAACAATT
    CCCCGCAATC TTCTAAAGAC AGATGCTGTC CTTTGGCAGT GGGCTATTTG GGAAGCAGCA
    CAGTTCACTG TCCTTTCTAA GTTGAGAACT CCACTGGGCA GAGCTCAGGA TACCTTCCAG
    ACAATTGAAG GTATCATCAG AAGTCTTGCA GCTCACACCT TAAACCCTGA TCAGGATGTT
    AGTCAGTGGA CAACTGCAGA CAATGATGAA GGCCACAGTA GCAACCAGCT TAGACTTGTG
    CTTCTTCTGC AGTATCTGGA AAACCTGGAG AAGTTAATGT ATAATGCATA TGAGGGATGT
    GCCAATGCGT TGACATCACC TCCCAAGGTC ATTAGGACTT TTTTCTATAC CAACCGCCAG
    ACTTGTCAAG ACTGGCTGAC CCGAATCCGA CTCTCCATCA TGAGGGTTGG ATTGTTAGCC
    GGCCAGCCTG CTGTGACAGT GAGACATGGC TTTGATTTGC TTACGGAAAT GAAGACAAAT
    CCATCTCAGG GGAATGAATT GGAAGTAACT ATCATGATGG TGGTAGAAGC ACTGTGTGAA
    CTTCATTGTC CAGAAGCTAT ACAAGGAATT GCTGTCTGGT CATCTTCCAT CGTTGGAAAA
    AATCTTCTCT GGATTAATTC AGTGGCCCAA CAGGCTGAAG GGAGGTTTGA AAAGGCCTCC
    GTGGAGTACC AGGAGCACCT GTGTGCCATG ACAGGTGTGG ATTGCTGCAT CTCCAGCTTT
    GACAAGTCTG TTCTCACATT GGCTAATACT GGGCGTAACA GTGCCAGCCC CAAACATTCT
    TTGAACGGTG AATCCAGAAA AACTGTGCTG TCCAAACCAA CTGACTCTTC TCCTGAGGTT
    ATAAATTATT TGGGAAATAA AGCATGTGAG TGCTACATTG CAATTGCCGA TTGGACTGCT
    GTACAGGAAT GGCAGAATGC TATCCATGAC TTGAAAAAGA GTACCAGCAG TACTTCTCTC
    AATCTAAAAG CTGATTTCAA CTACATAAAA TCACTAAGCA GTTTTGAATC TGGAGAATTT
    GTTGAATGTA CTGAGCAATT GGAATTGTTA CCAGGAGAAA ATATCAATCT ACTTGCTGGA
    GGATCAAAAG AAAAAATAGA TATGAAAAAA CTACTTCCTA ATATGTTAAG TCCAGATCCA
    AGAGAACTTC AGAAATCCAT CGAAGTTCAA TTGCTGAGAA GTTCTGTTTG TTTGGCAACT
    GCTTTAAACC ATATAGAACA GGATCAGAAG TGGCAGTCTA TAACTGAAAA CGTGGTGAAG
    TACTTGAAGC AAACCTCCCG CATAGCTATC GGGCCACTGA GACTTTCTAC TTTAACAGTT
    TCACAGTCTC TGCCAGTTCT AAGTACCTTG CAGCTGTATT GCTCTTCTGC TTTGGAGAAC
    ACAGTTTCCA ACAGACTTTC AACAGAGGAC TGTCTTATCC CACTCTTCAG TGAAGCTTTG
    CGTTCATGTA AACAGCATGA TGTCAGGCCA TGGATGCAGG CATTAAGATA CACCATGTAC
    CAGAGTCAGT TGTTGGAGAA AATAAAAGAA CAAACAGTTC CAATTAGAAG CCATCTCATG
    GAATTAGGTC TAACAGCAGC AAAATTTGCT AGAAAACGGG GGAATGTGTC ACTTGCAACA
    AGACTGCTGG CACAGTGCAG TGAGGTTCAG CTGGCAAAGA CCACGACTGC GCAGGATTTA
    GTCCAGCATT TTAAAAAGCT ATCAACTCAA GGTCAAGTGG ATGAAAAGTG GGGGCCTGAA
    CTTGACATTG AAAAAACCAA ATTGCTATAT ACAGCAGGCC AGTCAACACA CGCAATGGAG
    ATGCTGAGTT CTTGCGCCAT TTCGTTCTGC AAGTCTGCCA AAGCTGAGCA TGCTGTTGCC
    AAGTCCATTC TTACACTGGC CAAATGGATC CAGGCAGAAT GGAAAGAAAT ATCAGGGCAG
    CTGAAACAGG TTTACAGAGC TCAGCAGCAG CAAAACTTCA CAGGTCTTTC TACTTTATCT
    AAAAACATAC TCACTCTAAT TGAACTCCCA TCTGTTAATA CAGTGGAAGA AGAGTATCCT
    CGAATCGAGA GCGAATCTAC AGTACATATC GGAGTTGGAG AACCTGACTT CATTTTGGGA
    CAGTTGTATC ACCTATCTTC AGTACAGGCA CCTGAAGTAG CCAAATCTTG GGCAGCATTG
    GCTAGTTGGG CTTATAGATG GGGCAGAAAG GTCGTTGACA ATGCCAGTCA GGGAGAAGGT
    GTTCGTCTGT TGCCCAGAGA AAAATCTGAA GTTCAGAATC TGCTCCCAGA CACTATCACT
    GAAGAAGAAA AAGAGAGAAT ATATGGTATT CTTGGGCAGG CAGTGTGTCG GCCAGCAGGA
    ATTCAGGATG AAGATATAAC GCTTCAGATA ACAGAAAGTG AAGATAATGA GGAAGATGAT
    ATGGTTGATG TTATCTGGCG TCAGTTAATA TCGAGCTGCC CCTGGCTTTC AGAACTTGAT
    GAAAGTGCAA CAGAAGGCGT TATTAAAGTG TGGAGGAAAG TTGTAGATAG AATCTTCAGC
    CTGTACAAAC TGTCTTGCAG CGCCTATTTT ACTTTCCTTA AGCTCAATGC TGGTCAGATT
    CCTTTAGATG AAGATGACCC CAGGCTCCAT TTAAGTCACA GACCGGAGCA GAGCACGGAT
    GACGTGATTG TGATGGCCAC ATTGAGGCTG CTCAGACTGC TCGTGAAACA TGCCGGCGAG
    CTCAGGCAGT ATCTGGAACA TGGCTTGGAA ACAACACCCA CTGCTCCCTG GAGAGGAATT
    ATTCCACAAC TTTTCTCACG CTTAAACCAC CCTGAAGTGT ATGTGCGCCA AAGTATTTGT
    AACCTTCTCT GCCGTGTTGC TCAGGATTCC CCCCATCTCA TATTGTACCC TGCGATAGTG
    GGCACCATAT CGCTTAGCAG TGAGTCACAG GCTTCAGGAA ATAAGTTTTC CTCTGCAATT
    CCAACTTTAC TTGGCAATAT TCAAGGAGAA GAATTGCTGG TTTCTGAATG TGAGGGAGGA
    AGTCCTCCTG CTTCCCAAGA TAGCAATAAG GATGAACCGA AGAGTGGATT GAATGAAGAC
    CAAGCTATGA TGCAGGATTG CTACAGCAAG ATTGTAGATA AACTGTCCTC TGCAAACCCA
    ACTATGGTGT TACAGGTTCA GATGCTCGTG GCCGAGCTGC GCAGGGTCAC TGTTCTCTGG
    GATGAGCTCT GGTTGGGAGT TCTGTTACAG CAGCACATGT ATGTCCTGAG ACGAATTCAG
    CAGCTGGAAG ACGAAGTGAA GAGAGTCCAG AATAACAACA CCTTACGCAA AGAAGAGAAA
    ATTGCAATCA TGCGAGAGAA GCACACAGCT TTGATGAAGC CCATTGTGTT TGCTTTGGAG
    CACGTGAGGA GTATCACGGC GGCCCCTGCA GAAACGCCTC ATGAAAAGTG GTTTCAAGAT
    AACTATGGTG ATGCAATTGA AAATGCCCTA GAAAAACTGA AGACACCATC AAACCCTGCA
    AAGCCTGGAA GCAGTTGGAT TCCATTTAAA GAGATAATGC TAAGTTTGCA ACAGAGAGCA
    CAGAAACGTG CAAGCTACAT CTTGCGTCTT GAAGAAATCA GCCCGTGGCT GGCTGCCATG
    ACTAACACTG AAATTGCTCT TCCTGGAGAA GTCTCAGCCA GAGACACTGT CACAATCCAC
    AGTGTGGGCG GAACCATCAC AATCTTACCC ACCAAAACCA AGCCAAAGAA ACTTCTCTTT
    CTAGGATCGG ATGGGAAGAG CTATCCTTAT CTTTTCAAAG GATTGGAGGA CTTACATCTG
    GATGAGAGAA TAATGCAGTT TTTATCTATT GTAAATACTA TGTTTGCTAC AATCAATCGC
    CAGGAGACAC CCCGCTTCCA TGCGCGGCAC TATTCTGTAA CACCACTGGG AACTCGATCA
    GGACTGATCC AGTGGGTGGA TGGAGCCACA CCCTTATTTG GTCTTTACAA ACGATGGCAA
    CAACGGGAAG CTGCCTTACA AGCACAAAAG GCCCAAGATT CCTACCAGAC TCCTCAGAAT
    CCGGGAATTG TACCCCGTCC TAGTGAACTC TATTATAGTA AAATCGGTCC TGCTTTGAAA
    GCAGTGGGAC TTAGTCTGGA TGTGTCCCGA CGGGATTGGC CTCTTCATGT GATGAAGGCA
    GTGCTAGAAG AGTTGATGGA AGCCACGCCT CCAAATCTCC TTGCTAAAGA GCTGTGGTCA
    TCTTGTACCA CCCCTGATGA ATGGTGGAGA GTCACACAGT CTTACGCCAG ATCGACCGCG
    GTCATGTCTA TGGTCGGGTA CATAATTGGC CTTGGAGACA GACATCTGGA TAACGTCCTC
    ATAGATATGA CGACTGGAGA GGTTGTTCAC ATAGATTACA ATGTTTGCTT TGAAAAAGGT
    AAAAGCCTTA GAGTTCCTGA GAAAGTTCCT TTTCGAATGA CACAGAACAT TGAAACAGCA
    CTGGGTGTAA CTGGAGTAGA AGGTGTTTTT AGACTTTCAT GTGAGCAGGT TTTGCACATC
    ATGCGGCGTG GCAGAGAGAC TCTGCTGACA CTACTGGAGG CCTTTGTGTA CGACCCCCTG
    GTGGACTGGA CAGCTGGGGG CGAGGCTGGG TTTGCTGGTG CCGTCTACGG TGGGGGAGGC
    CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAAA GAGAAATCAC CCGCAGCCTG
    TTTTCTTCGA GAGTAGCTGA AATTAAGGTG AACTGGTTTA AGAATAGGGA CGAGATGCTG
    GTTGTACTGC CCAAGCTGGA CAGCAGCTTA GATGAATACC TGAGCTTGCA AGAGCAACTG
    ACAGATGTGG AAAAACTGCA GGGCAAACTT CTGGAAGAAA TAGAGTTTCT AGAAGGAGCT
    GAAGGAGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAACA CACTCAGCTA
    CAGACTCAGC AGAGAGCTGT TCAGGAGGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAG
    TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT GGCAAGTTTG
    CTTCAGGAGA TAAGCACACA GATGGACCTA GGTCCTCCAA GCTATGTGCC AGCAACAGCC
    TTTCTTCAGA ACGCTGGCCA GGCCCACTTG ATTAGCCAGT GTGAGCAGTT AGAGGGGGAA
    GTTGGCGCCC TCCTGCAGCA GAGGCGCTCG GTGCTGCGAG GCTGTCTGGA GCAGCTGCAT
    CACTATGCAA CTGTAGCTCT GCAATATCCA AAGGCCATAT TTCAGAAACA TCGAATTGAG
    CAGTGGAAGA CCTGGATGGA AGAACTTATC TGTAACACCA CTGTAGAGCG CTGTCAGGAA
    CTTTACAGGA AATATGAAAT GCAGTATGCA CCCCAGCCGC CCCCAACAGT GTGTCAGTTC
    ATCACTGCCA CTGAGATGAC CCTGCAGCGG TACGCAGCAG ATATAAACAG TAGGCTTATT
    AGACAGGTGG AACGATTGAA ACAAGAAGCC GTCACCGTAC CTGTTTGTGA AGATCAGCTG
    AAAGAAATTG AACGTTGCAT CAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG
    AGTCTAGCAA GTGTTATTAT CTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG
    GAAGGGGCAG CTTCGAGTGC TGGAGAGCAG TTGGTGGATC TGACTTCTCG GGATGGAGCC
    TGGTTCTTGG AGGAGCTCTG CAGTATGAGT GGAAATGTCA CTTGCCTTGT TCAGTTACTG
    AAGCAGTGTC ATCTGGTGCC ACAGGACTTA GACATCCCTA ACCCCATGGA GGCGTCTGAG
    GCAGTTCACT TAGCTAATGG AGTATACACC TCACTTCAGG AATTAAATTC AAATTTCCGG
    CAAATCATAT TTCCAGAAGC ACTGAGATGT TTAATGAAAG GCGAATACAC ATTAGAAAGT
    ATGCTTCATG AACTGGACAG CCTTATTGAG CAGACCACTG ATGGCGTTCC TCTGCAGACT
    CTAGTGGAAT CTCTTCAAGC CTATTTAAGA AACGCAGCCA TGGGACTTGA AGAAGAAACA
    CATGCCCATT ACATCGATGT TGCCAGAATA CTTCATGCTC AATATGGTGA GTTAATCCAA
    CCAAGAAATG GTTCAGTTGA TGAAACACCA AAAATGTCAG CTGGCCAGAT GCTTTTGGTA
    GCCTTTGATG GCATGTTTGC CCAAGTTGAG ACTGCTTTTG GCTTATTAGT TGAAAAGCTA
    AACAAGATGG AAATTCCTAT CGCTTGGAGA AAGATTGACA TTATAAGGGA AGCCAGGAGC
    ACTCAAGTCA ACTTTTTTGA TGATGATAAT CACCGACAGG TGCTGGAAGA GATTTTCTTT
    CTAAAACGAC TGCAGACAAT TAAGGAGTTC TTCAGACTCT GCGGTACCTT TTCTAAAACT
    TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTAA ATGGGCCTGT ACCGATTGTC
    AACGTGAAAA CTCTTTTTAG GAACTCTTGT TTCAGTGAAG ACCAGATGGC CAAACCTATA
    AAGGCCTTCA CAGCTGACTT CGTGCGGCAG CTCCTGATTG GACTCCCGAA CCAAGCCCTG
    GGCCTCACGC TGTGCAGCTT CATCAGTGCC CTAGGAGTGG ACATCATTGC TCAAGTGGAG
    GCCAAGGATT TTGGTGCTGA AAGCAAGGTT TCTGTTGATG ATCTGTGTAA GAAAGCAGTG
    GAGCACAACA TCCAGGTCGG GAAGTTCTCT CAGCTGGTCA TGAACAGGGC GACTGTGCTC
    GCCAGCTCCT ATGACACTGC CTGGAAGAAG CACGACTTGG TGCGCAGGCT GGAGACCAGT
    ATCTCCTCCT GTAAGACGAG CCTGCAGCGC GTTCAGCTGC ATATCGCCAT GTTTCAGTGG
    CAGCATGAAG ATCTCCTTAT CAACAGACCA CAAGCGATGT CGGTCACACC TCCCCCACGA
    TCTGCCATCC TAACCAGCAT GAAAAAGAAA CTCCATACTC TGAGCCAGAT TGAAACTTCA
    ATTGCAACAG TTCAGGAGAA ACTGGCAGCT CTTGAAGCAA GTATTGAACA GCGACTCAAG
    TGGGCAGGTG GTGCCAACCC TGCACTGGCA CCAGTACTAC AAGATTTTGA AGCAACAATA
    GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CCAGTCAGGT CACTTTCCTC
    TGCAGCAATA TTATTCATTT TGAAAGTTTA CGAACTAGAA CTGCGGAAGC CTTAAACCTG
    GATGCTGCAT TATTTGAGCT AATCAAACGG TGTCAGCAAA TGTGTTCATT TGCTTCACAG
    TTTAACAGTT CAGTCTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACAAGCCTT
    GAACACCCTA TTGGCAGCTC TGAATGGCTT TTATCAGCAC ACAAACAGTT GACCCAGGAT
    ATGTCTACGC AGAGAGCAAT TCAGACGGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA
    ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CAGGTCATAA CCGGCAGCTT
    GGAGATGTCA AACATCTCCT GAAAGCAATG GCCAAGGATG AAGAAGCTGC TCTGGCAGAT
    GGCGAAGATG TTCCCTATGA GAACAGTGTG AGGCAGTTCT TGGGTGAATA TAAATCTTGG
    CAAGACAACA TTCAGACTGT GCTGTTTACA TTAGTCCAAG CCATGGGTCA GGTTCGAAGT
    CAGGAACATG TTGAAATGCT CCAGGAAATA ACTCCCACCT TGAAAGAACT GAAAACACAA
    AGTCAGAGTG TCTATAATAA TTTAGTGAGT TTTGCATCAC CACTAGTCAC CGATGCAACA
    AATGAATGTT CAAGTCCAAC GTCATCTGCT ACGTATCAGC CATCCTTTGC TGCAGTCCGG
    AGCAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCAC AGAATGCTAG AAAGCTGATT
    CAGAAAAATC TAGCTACATC AGCAGATACT CCACCAAGCA CTGTTCCAGG AACTGGCAAG
    AGTGCTGCTT GTAGTCCTAA AAAGGCCGTC AGAGACCCTA AAACTGGGAA AGCTGTGCAA
    GAGAGAAATT CATATGCAGT GAGCGTGTGG AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA
    GATGTTGATC CGAATAGGAG GATGTCAGTT GCTGAACAGG TTGACTATGT CATTAAGGAA
    GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe245764
    Clone ID Related Accession (Same CDS sequence) XM_021086549.1 , XM_021086550.1
    Accession Version XM_021086549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product serine/threonine-protein kinase SMG1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAT TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAACCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA CGATGATACC
    AGAATCCACG CTGACATACA AAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TCACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGACGA CAGAGACCGA AGACTGGCTA CTGTAAAGCA GTTGAAAGAG
    TTTATTCAGC AACCAGAAAA TAAGCTGGTA CTAGTTAAAC AATTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT CTGAGCTATG AGGCCGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TGAAACTTCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAATGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACTAAT
    TTTAGGGATA CAGTTGATAT ATTAGTCGGA TGGCATATAG ATCATACTCA GAAGCCTTCC
    CTCACACAGC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGATCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT CTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TGGCCTCCGG GGAGTCAGTG GATGAAGACG TTCCTCCTCC GTCAGTGTCC
    TTACCAAAGC TGGCAGCTCT CCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGGGG TCCTCCAATT ACAGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAGGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TCATTACATA TGGATTAGAT CAGCTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    TTGCCTTCAT CATTTGTAGA AAAACTATTT ATACCATCAT CTAAACTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGC
    CTGAAGAATA TTCCTGTTTT GGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ACCTGCTACA TAGTCTACAA CTTCCCGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATTT ATTCAACGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTCAGTGCTC TGACTACAAT TGGGAATGCT AAAAATTCAC TAATTGGGAT GTGGGCGCTG
    TCTCCAACCG TCTTTGCACT CCTGAGTAAG AATCTGATGA TTGTGCATGG TGACCTGGCC
    GTTCACTTCC CTGCCATTCA GTATGCAGTG CTCTACACGT TGTATTCACA TTGTACTAGG
    CATGACCACT TTATATCTAG TAGTCTGAGT TCTTCGTCCC CATCTTTGTT TGATGGAGCC
    GTGATTAGTA CTGTAACCAC AGCCACCAAA AAACATTTCT CAATTATATT AAATCTCTTG
    GGAATATTAC TAAAGAAGGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ACGCACCTTT ATTCTCTCTG
    CCATCTTTCC ATAAGTTTTC CAAAGGCCTT TTAGCCAACA CTCTTGTTGA AGATGTGAAT
    ATCTGTCTGC AAGCCTGCAG CAGTCTACAT GCTTTGTCCT CGTCTTTACC GGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAACTAGTCC ATAGTGGAAC TCGTATTCGC
    CAGGCATTTG GAAAGCTATT GAAGTCAATT CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AATACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGGAACTCT
    CATAGAACGG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ACAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACAAT TCCCCGCAAT CTTCTAAAGA CAGATGCTGT CCTTTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGTTGAGAAC TCCACTGGGC
    AGAGCTCAGG ATACCTTCCA GACAATTGAA GGTATCATCA GAAGTCTTGC AGCTCACACC
    TTAAACCCTG ATCAGGATGT TAGTCAGTGG ACAACTGCAG ACAATGATGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTTCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAGGGATG TGCCAATGCG TTGACATCAC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAACCGCCA GACTTGTCAA GACTGGCTGA CCCGAATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTAGC CGGCCAGCCT GCTGTGACAG TGAGACATGG CTTTGATTTG
    CTTACGGAAA TGAAGACAAA TCCATCTCAG GGGAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CACTGTGTGA ACTTCATTGT CCAGAAGCTA TACAAGGAAT TGCTGTCTGG
    TCATCTTCCA TCGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC CGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTG
    GATTGCTGCA TCTCCAGCTT TGACAAGTCT GTTCTCACAT TGGCTAATAC TGGGCGTAAC
    AGTGCCAGCC CCAAACATTC TTTGAACGGT GAATCCAGAA AAACTGTGCT GTCCAAACCA
    ACTGACTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    GCAATTGCCG ATTGGACTGC TGTACAGGAA TGGCAGAATG CTATCCATGA CTTGAAAAAG
    AGTACCAGCA GTACTTCTCT CAATCTAAAA GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TGTTGAATGT ACTGAGCAAT TGGAATTGTT ACCAGGAGAA
    AATATCAATC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTACTTCCT
    AATATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TCGAAGTTCA ATTGCTGAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CATATAGAAC AGGATCAGAA GTGGCAGTCT
    ATAACTGAAA ACGTGGTGAA GTACTTGAAG CAAACCTCCC GCATAGCTAT CGGGCCACTG
    AGACTTTCTA CTTTAACAGT TTCACAGTCT CTGCCAGTTC TAAGTACCTT GCAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTT CAACAGAGGA CTGTCTTATC
    CCACTCTTCA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAG
    GCATTAAGAT ACACCATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGACTGCTG GCACAGTGCA GTGAGGTTCA GCTGGCAAAG
    ACCACGACTG CGCAGGATTT AGTCCAGCAT TTTAAAAAGC TATCAACTCA AGGTCAAGTG
    GATGAAAAGT GGGGGCCTGA ACTTGACATT GAAAAAACCA AATTGCTATA TACAGCAGGC
    CAGTCAACAC ACGCAATGGA GATGCTGAGT TCTTGCGCCA TTTCGTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCCATT CTTACACTGG CCAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TATCAGGGCA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAAAACTTC
    ACAGGTCTTT CTACTTTATC TAAAAACATA CTCACTCTAA TTGAACTCCC ATCTGTTAAT
    ACAGTGGAAG AAGAGTATCC TCGAATCGAG AGCGAATCTA CAGTACATAT CGGAGTTGGA
    GAACCTGACT TCATTTTGGG ACAGTTGTAT CACCTATCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCAGCATT GGCTAGTTGG GCTTATAGAT GGGGCAGAAA GGTCGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCCAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACTATCAC TGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGGCAG
    GCAGTGTGTC GGCCAGCAGG AATTCAGGAT GAAGATATAA CGCTTCAGAT AACAGAAAGT
    GAAGATAATG AGGAAGATGA TATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCGAGCTGC
    CCCTGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGCG TTATTAAAGT GTGGAGGAAA
    GTTGTAGATA GAATCTTCAG CCTGTACAAA CTGTCTTGCA GCGCCTATTT TACTTTCCTT
    AAGCTCAATG CTGGTCAGAT TCCTTTAGAT GAAGATGACC CCAGGCTCCA TTTAAGTCAC
    AGACCGGAGC AGAGCACGGA TGACGTGATT GTGATGGCCA CATTGAGGCT GCTCAGACTG
    CTCGTGAAAC ATGCCGGCGA GCTCAGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCC
    ACTGCTCCCT GGAGAGGAAT TATTCCACAA CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AAAGTATTTG TAACCTTCTC TGCCGTGTTG CTCAGGATTC CCCCCATCTC
    ATATTGTACC CTGCGATAGT GGGCACCATA TCGCTTAGCA GTGAGTCACA GGCTTCAGGA
    AATAAGTTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAATTGCTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCCCAAG ATAGCAATAA GGATGAACCG
    AAGAGTGGAT TGAATGAAGA CCAAGCTATG ATGCAGGATT GCTACAGCAA GATTGTAGAT
    AAACTGTCCT CTGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTCGT GGCCGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGCTGGAA GACGAAGTGA AGAGAGTCCA GAATAACAAC
    ACCTTACGCA AAGAAGAGAA AATTGCAATC ATGCGAGAGA AGCACACAGC TTTGATGAAG
    CCCATTGTGT TTGCTTTGGA GCACGTGAGG AGTATCACGG CGGCCCCTGC AGAAACGCCT
    CATGAAAAGT GGTTTCAAGA TAACTATGGT GATGCAATTG AAAATGCCCT AGAAAAACTG
    AAGACACCAT CAAACCCTGC AAAGCCTGGA AGCAGTTGGA TTCCATTTAA AGAGATAATG
    CTAAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGCTACA TCTTGCGTCT TGAAGAAATC
    AGCCCGTGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCTGGAGA AGTCTCAGCC
    AGAGACACTG TCACAATCCA CAGTGTGGGC GGAACCATCA CAATCTTACC CACCAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCG GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ACTTACATCT GGATGAGAGA ATAATGCAGT TTTTATCTAT TGTAAATACT
    ATGTTTGCTA CAATCAATCG CCAGGAGACA CCCCGCTTCC ATGCGCGGCA CTATTCTGTA
    ACACCACTGG GAACTCGATC AGGACTGATC CAGTGGGTGG ATGGAGCCAC ACCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCTGCCTTAC AAGCACAAAA GGCCCAAGAT
    TCCTACCAGA CTCCTCAGAA TCCGGGAATT GTACCCCGTC CTAGTGAACT CTATTATAGT
    AAAATCGGTC CTGCTTTGAA AGCAGTGGGA CTTAGTCTGG ATGTGTCCCG ACGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGCTAGAA GAGTTGATGG AAGCCACGCC TCCAAATCTC
    CTTGCTAAAG AGCTGTGGTC ATCTTGTACC ACCCCTGATG AATGGTGGAG AGTCACACAG
    TCTTACGCCA GATCGACCGC GGTCATGTCT ATGGTCGGGT ACATAATTGG CCTTGGAGAC
    AGACATCTGG ATAACGTCCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTTCC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGACTTTCA
    TGTGAGCAGG TTTTGCACAT CATGCGGCGT GGCAGAGAGA CTCTGCTGAC ACTACTGGAG
    GCCTTTGTGT ACGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCCGTCTACG GTGGGGGAGG CCAGCAGGCC GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    AGAGAAATCA CCCGCAGCCT GTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAGAATAGGG ACGAGATGCT GGTTGTACTG CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT GACAGATGTG GAAAAACTGC AGGGCAAACT TCTGGAAGAA
    ATAGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAGCT ACAGACTCAG CAGAGAGCTG TTCAGGAGGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CATTATCAGG CTGCATTCAA TAATTTAGAA
    GCAACACAGC TGGCAAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT AGGTCCTCCA
    AGCTATGTGC CAGCAACAGC CTTTCTTCAG AACGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGT TAGAGGGGGA AGTTGGCGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTATGCA ACTGTAGCTC TGCAATATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAACTTAT CTGTAACACC
    ACTGTAGAGC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC ACCCCAGCCG
    CCCCCAACAG TGTGTCAGTT CATCACTGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCA
    GATATAAACA GTAGGCTTAT TAGACAGGTG GAACGATTGA AACAAGAAGC CGTCACCGTA
    CCTGTTTGTG AAGATCAGCT GAAAGAAATT GAACGTTGCA TCAAAGTTTT CCTTCATGAG
    AATGGAGAAG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TCTCTGCCCT TTGTACCCTT
    ACAAGGCGTA ACCTGATGAT GGAAGGGGCA GCTTCGAGTG CTGGAGAGCA GTTGGTGGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAGCTCT GCAGTATGAG TGGAAATGTC
    ACTTGCCTTG TTCAGTTACT GAAGCAGTGT CATCTGGTGC CACAGGACTT AGACATCCCT
    AACCCCATGG AGGCGTCTGA GGCAGTTCAC TTAGCTAATG GAGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGCGAATACA CATTAGAAAG TATGCTTCAT GAACTGGACA GCCTTATTGA GCAGACCACT
    GATGGCGTTC CTCTGCAGAC TCTAGTGGAA TCTCTTCAAG CCTATTTAAG AAACGCAGCC
    ATGGGACTTG AAGAAGAAAC ACATGCCCAT TACATCGATG TTGCCAGAAT ACTTCATGCT
    CAATATGGTG AGTTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCCTTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTAG TTGAAAAGCT AAACAAGATG GAAATTCCTA TCGCTTGGAG AAAGATTGAC
    ATTATAAGGG AAGCCAGGAG CACTCAAGTC AACTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATTTTCTT TCTAAAACGA CTGCAGACAA TTAAGGAGTT CTTCAGACTC
    TGCGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACCGATTGT CAACGTGAAA ACTCTTTTTA GGAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCTAT AAAGGCCTTC ACAGCTGACT TCGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACG CTGTGCAGCT TCATCAGTGC CCTAGGAGTG
    GACATCATTG CTCAAGTGGA GGCCAAGGAT TTTGGTGCTG AAAGCAAGGT TTCTGTTGAT
    GATCTGTGTA AGAAAGCAGT GGAGCACAAC ATCCAGGTCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT CGCCAGCTCC TATGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG TATCTCCTCC TGTAAGACGA GCCTGCAGCG CGTTCAGCTG
    CATATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAACAGACC ACAAGCGATG
    TCGGTCACAC CTCCCCCACG ATCTGCCATC CTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC TCTTGAAGCA
    AGTATTGAAC AGCGACTCAA GTGGGCAGGT GGTGCCAACC CTGCACTGGC ACCAGTACTA
    CAAGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAAGA GAGCCAAAGA
    GCCAGTCAGG TCACTTTCCT CTGCAGCAAT ATTATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCGGAAG CCTTAAACCT GGATGCTGCA TTATTTGAGC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCTTCACA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGAGTGG ACACAAGCCT TGAACACCCT ATTGGCAGCT CTGAATGGCT TTTATCAGCA
    CACAAACAGT TGACCCAGGA TATGTCTACG CAGAGAGCAA TTCAGACGGA GAAAGAGCAG
    CAGATAGAAA CGGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACAGGTCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGCGAAGAT GTTCCCTATG AGAACAGTGT GAGGCAGTTC
    TTGGGTGAAT ATAAATCTTG GCAAGACAAC ATTCAGACTG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAGGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACACA AAGTCAGAGT GTCTATAATA ATTTAGTGAG TTTTGCATCA
    CCACTAGTCA CCGATGCAAC AAATGAATGT TCAAGTCCAA CGTCATCTGC TACGTATCAG
    CCATCCTTTG CTGCAGCAGT CCGGAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAATG CTAGAAAGCT GATTCAGAAA AATCTAGCTA CATCAGCAGA TACTCCACCA
    AGCACTGTTC CAGGAACTGG CAAGAGTGCT GCTTGTAGTC CTAAAAAGGC CGTCAGAGAC
    CCTAAAACTG GGAAAGCTGT GCAAGAGAGA AATTCATATG CAGTGAGCGT GTGGAAGAGA
    GTGAAAGCCA AGTTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020942208.1
    CDS457..11397
    Translation

    Target ORF information:

    RefSeq Version XM_021086549.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021086549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAT TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAACCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA CGATGATACC
    AGAATCCACG CTGACATACA AAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TCACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGACGA CAGAGACCGA AGACTGGCTA CTGTAAAGCA GTTGAAAGAG
    TTTATTCAGC AACCAGAAAA TAAGCTGGTA CTAGTTAAAC AATTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT CTGAGCTATG AGGCCGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TGAAACTTCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAATGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACTAAT
    TTTAGGGATA CAGTTGATAT ATTAGTCGGA TGGCATATAG ATCATACTCA GAAGCCTTCC
    CTCACACAGC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGATCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT CTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TGGCCTCCGG GGAGTCAGTG GATGAAGACG TTCCTCCTCC GTCAGTGTCC
    TTACCAAAGC TGGCAGCTCT CCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGGGG TCCTCCAATT ACAGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAGGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TCATTACATA TGGATTAGAT CAGCTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    TTGCCTTCAT CATTTGTAGA AAAACTATTT ATACCATCAT CTAAACTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGC
    CTGAAGAATA TTCCTGTTTT GGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ACCTGCTACA TAGTCTACAA CTTCCCGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATTT ATTCAACGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTCAGTGCTC TGACTACAAT TGGGAATGCT AAAAATTCAC TAATTGGGAT GTGGGCGCTG
    TCTCCAACCG TCTTTGCACT CCTGAGTAAG AATCTGATGA TTGTGCATGG TGACCTGGCC
    GTTCACTTCC CTGCCATTCA GTATGCAGTG CTCTACACGT TGTATTCACA TTGTACTAGG
    CATGACCACT TTATATCTAG TAGTCTGAGT TCTTCGTCCC CATCTTTGTT TGATGGAGCC
    GTGATTAGTA CTGTAACCAC AGCCACCAAA AAACATTTCT CAATTATATT AAATCTCTTG
    GGAATATTAC TAAAGAAGGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ACGCACCTTT ATTCTCTCTG
    CCATCTTTCC ATAAGTTTTC CAAAGGCCTT TTAGCCAACA CTCTTGTTGA AGATGTGAAT
    ATCTGTCTGC AAGCCTGCAG CAGTCTACAT GCTTTGTCCT CGTCTTTACC GGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAACTAGTCC ATAGTGGAAC TCGTATTCGC
    CAGGCATTTG GAAAGCTATT GAAGTCAATT CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AATACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGGAACTCT
    CATAGAACGG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ACAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACAAT TCCCCGCAAT CTTCTAAAGA CAGATGCTGT CCTTTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGTTGAGAAC TCCACTGGGC
    AGAGCTCAGG ATACCTTCCA GACAATTGAA GGTATCATCA GAAGTCTTGC AGCTCACACC
    TTAAACCCTG ATCAGGATGT TAGTCAGTGG ACAACTGCAG ACAATGATGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTTCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAGGGATG TGCCAATGCG TTGACATCAC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAACCGCCA GACTTGTCAA GACTGGCTGA CCCGAATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTAGC CGGCCAGCCT GCTGTGACAG TGAGACATGG CTTTGATTTG
    CTTACGGAAA TGAAGACAAA TCCATCTCAG GGGAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CACTGTGTGA ACTTCATTGT CCAGAAGCTA TACAAGGAAT TGCTGTCTGG
    TCATCTTCCA TCGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC CGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTG
    GATTGCTGCA TCTCCAGCTT TGACAAGTCT GTTCTCACAT TGGCTAATAC TGGGCGTAAC
    AGTGCCAGCC CCAAACATTC TTTGAACGGT GAATCCAGAA AAACTGTGCT GTCCAAACCA
    ACTGACTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    GCAATTGCCG ATTGGACTGC TGTACAGGAA TGGCAGAATG CTATCCATGA CTTGAAAAAG
    AGTACCAGCA GTACTTCTCT CAATCTAAAA GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TGTTGAATGT ACTGAGCAAT TGGAATTGTT ACCAGGAGAA
    AATATCAATC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTACTTCCT
    AATATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TCGAAGTTCA ATTGCTGAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CATATAGAAC AGGATCAGAA GTGGCAGTCT
    ATAACTGAAA ACGTGGTGAA GTACTTGAAG CAAACCTCCC GCATAGCTAT CGGGCCACTG
    AGACTTTCTA CTTTAACAGT TTCACAGTCT CTGCCAGTTC TAAGTACCTT GCAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTT CAACAGAGGA CTGTCTTATC
    CCACTCTTCA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAG
    GCATTAAGAT ACACCATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGACTGCTG GCACAGTGCA GTGAGGTTCA GCTGGCAAAG
    ACCACGACTG CGCAGGATTT AGTCCAGCAT TTTAAAAAGC TATCAACTCA AGGTCAAGTG
    GATGAAAAGT GGGGGCCTGA ACTTGACATT GAAAAAACCA AATTGCTATA TACAGCAGGC
    CAGTCAACAC ACGCAATGGA GATGCTGAGT TCTTGCGCCA TTTCGTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCCATT CTTACACTGG CCAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TATCAGGGCA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAAAACTTC
    ACAGGTCTTT CTACTTTATC TAAAAACATA CTCACTCTAA TTGAACTCCC ATCTGTTAAT
    ACAGTGGAAG AAGAGTATCC TCGAATCGAG AGCGAATCTA CAGTACATAT CGGAGTTGGA
    GAACCTGACT TCATTTTGGG ACAGTTGTAT CACCTATCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCAGCATT GGCTAGTTGG GCTTATAGAT GGGGCAGAAA GGTCGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCCAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACTATCAC TGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGGCAG
    GCAGTGTGTC GGCCAGCAGG AATTCAGGAT GAAGATATAA CGCTTCAGAT AACAGAAAGT
    GAAGATAATG AGGAAGATGA TATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCGAGCTGC
    CCCTGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGCG TTATTAAAGT GTGGAGGAAA
    GTTGTAGATA GAATCTTCAG CCTGTACAAA CTGTCTTGCA GCGCCTATTT TACTTTCCTT
    AAGCTCAATG CTGGTCAGAT TCCTTTAGAT GAAGATGACC CCAGGCTCCA TTTAAGTCAC
    AGACCGGAGC AGAGCACGGA TGACGTGATT GTGATGGCCA CATTGAGGCT GCTCAGACTG
    CTCGTGAAAC ATGCCGGCGA GCTCAGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCC
    ACTGCTCCCT GGAGAGGAAT TATTCCACAA CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AAAGTATTTG TAACCTTCTC TGCCGTGTTG CTCAGGATTC CCCCCATCTC
    ATATTGTACC CTGCGATAGT GGGCACCATA TCGCTTAGCA GTGAGTCACA GGCTTCAGGA
    AATAAGTTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAATTGCTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCCCAAG ATAGCAATAA GGATGAACCG
    AAGAGTGGAT TGAATGAAGA CCAAGCTATG ATGCAGGATT GCTACAGCAA GATTGTAGAT
    AAACTGTCCT CTGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTCGT GGCCGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGCTGGAA GACGAAGTGA AGAGAGTCCA GAATAACAAC
    ACCTTACGCA AAGAAGAGAA AATTGCAATC ATGCGAGAGA AGCACACAGC TTTGATGAAG
    CCCATTGTGT TTGCTTTGGA GCACGTGAGG AGTATCACGG CGGCCCCTGC AGAAACGCCT
    CATGAAAAGT GGTTTCAAGA TAACTATGGT GATGCAATTG AAAATGCCCT AGAAAAACTG
    AAGACACCAT CAAACCCTGC AAAGCCTGGA AGCAGTTGGA TTCCATTTAA AGAGATAATG
    CTAAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGCTACA TCTTGCGTCT TGAAGAAATC
    AGCCCGTGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCTGGAGA AGTCTCAGCC
    AGAGACACTG TCACAATCCA CAGTGTGGGC GGAACCATCA CAATCTTACC CACCAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCG GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ACTTACATCT GGATGAGAGA ATAATGCAGT TTTTATCTAT TGTAAATACT
    ATGTTTGCTA CAATCAATCG CCAGGAGACA CCCCGCTTCC ATGCGCGGCA CTATTCTGTA
    ACACCACTGG GAACTCGATC AGGACTGATC CAGTGGGTGG ATGGAGCCAC ACCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCTGCCTTAC AAGCACAAAA GGCCCAAGAT
    TCCTACCAGA CTCCTCAGAA TCCGGGAATT GTACCCCGTC CTAGTGAACT CTATTATAGT
    AAAATCGGTC CTGCTTTGAA AGCAGTGGGA CTTAGTCTGG ATGTGTCCCG ACGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGCTAGAA GAGTTGATGG AAGCCACGCC TCCAAATCTC
    CTTGCTAAAG AGCTGTGGTC ATCTTGTACC ACCCCTGATG AATGGTGGAG AGTCACACAG
    TCTTACGCCA GATCGACCGC GGTCATGTCT ATGGTCGGGT ACATAATTGG CCTTGGAGAC
    AGACATCTGG ATAACGTCCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTTCC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGACTTTCA
    TGTGAGCAGG TTTTGCACAT CATGCGGCGT GGCAGAGAGA CTCTGCTGAC ACTACTGGAG
    GCCTTTGTGT ACGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCCGTCTACG GTGGGGGAGG CCAGCAGGCC GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    AGAGAAATCA CCCGCAGCCT GTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAGAATAGGG ACGAGATGCT GGTTGTACTG CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT GACAGATGTG GAAAAACTGC AGGGCAAACT TCTGGAAGAA
    ATAGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAGCT ACAGACTCAG CAGAGAGCTG TTCAGGAGGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CATTATCAGG CTGCATTCAA TAATTTAGAA
    GCAACACAGC TGGCAAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT AGGTCCTCCA
    AGCTATGTGC CAGCAACAGC CTTTCTTCAG AACGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGT TAGAGGGGGA AGTTGGCGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTATGCA ACTGTAGCTC TGCAATATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAACTTAT CTGTAACACC
    ACTGTAGAGC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC ACCCCAGCCG
    CCCCCAACAG TGTGTCAGTT CATCACTGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCA
    GATATAAACA GTAGGCTTAT TAGACAGGTG GAACGATTGA AACAAGAAGC CGTCACCGTA
    CCTGTTTGTG AAGATCAGCT GAAAGAAATT GAACGTTGCA TCAAAGTTTT CCTTCATGAG
    AATGGAGAAG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TCTCTGCCCT TTGTACCCTT
    ACAAGGCGTA ACCTGATGAT GGAAGGGGCA GCTTCGAGTG CTGGAGAGCA GTTGGTGGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAGCTCT GCAGTATGAG TGGAAATGTC
    ACTTGCCTTG TTCAGTTACT GAAGCAGTGT CATCTGGTGC CACAGGACTT AGACATCCCT
    AACCCCATGG AGGCGTCTGA GGCAGTTCAC TTAGCTAATG GAGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGCGAATACA CATTAGAAAG TATGCTTCAT GAACTGGACA GCCTTATTGA GCAGACCACT
    GATGGCGTTC CTCTGCAGAC TCTAGTGGAA TCTCTTCAAG CCTATTTAAG AAACGCAGCC
    ATGGGACTTG AAGAAGAAAC ACATGCCCAT TACATCGATG TTGCCAGAAT ACTTCATGCT
    CAATATGGTG AGTTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCCTTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTAG TTGAAAAGCT AAACAAGATG GAAATTCCTA TCGCTTGGAG AAAGATTGAC
    ATTATAAGGG AAGCCAGGAG CACTCAAGTC AACTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATTTTCTT TCTAAAACGA CTGCAGACAA TTAAGGAGTT CTTCAGACTC
    TGCGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACCGATTGT CAACGTGAAA ACTCTTTTTA GGAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCTAT AAAGGCCTTC ACAGCTGACT TCGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACG CTGTGCAGCT TCATCAGTGC CCTAGGAGTG
    GACATCATTG CTCAAGTGGA GGCCAAGGAT TTTGGTGCTG AAAGCAAGGT TTCTGTTGAT
    GATCTGTGTA AGAAAGCAGT GGAGCACAAC ATCCAGGTCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT CGCCAGCTCC TATGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG TATCTCCTCC TGTAAGACGA GCCTGCAGCG CGTTCAGCTG
    CATATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAACAGACC ACAAGCGATG
    TCGGTCACAC CTCCCCCACG ATCTGCCATC CTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC TCTTGAAGCA
    AGTATTGAAC AGCGACTCAA GTGGGCAGGT GGTGCCAACC CTGCACTGGC ACCAGTACTA
    CAAGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAAGA GAGCCAAAGA
    GCCAGTCAGG TCACTTTCCT CTGCAGCAAT ATTATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCGGAAG CCTTAAACCT GGATGCTGCA TTATTTGAGC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCTTCACA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGAGTGG ACACAAGCCT TGAACACCCT ATTGGCAGCT CTGAATGGCT TTTATCAGCA
    CACAAACAGT TGACCCAGGA TATGTCTACG CAGAGAGCAA TTCAGACGGA GAAAGAGCAG
    CAGATAGAAA CGGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACAGGTCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGCGAAGAT GTTCCCTATG AGAACAGTGT GAGGCAGTTC
    TTGGGTGAAT ATAAATCTTG GCAAGACAAC ATTCAGACTG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAGGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACACA AAGTCAGAGT GTCTATAATA ATTTAGTGAG TTTTGCATCA
    CCACTAGTCA CCGATGCAAC AAATGAATGT TCAAGTCCAA CGTCATCTGC TACGTATCAG
    CCATCCTTTG CTGCAGCAGT CCGGAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAATG CTAGAAAGCT GATTCAGAAA AATCTAGCTA CATCAGCAGA TACTCCACCA
    AGCACTGTTC CAGGAACTGG CAAGAGTGCT GCTTGTAGTC CTAAAAAGGC CGTCAGAGAC
    CCTAAAACTG GGAAAGCTGT GCAAGAGAGA AATTCATATG CAGTGAGCGT GTGGAAGAGA
    GTGAAAGCCA AGTTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe245764
    Clone ID Related Accession (Same CDS sequence) XM_021086549.1 , XM_021086550.1
    Accession Version XM_021086550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product serine/threonine-protein kinase SMG1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAT TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAACCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA CGATGATACC
    AGAATCCACG CTGACATACA AAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TCACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGACGA CAGAGACCGA AGACTGGCTA CTGTAAAGCA GTTGAAAGAG
    TTTATTCAGC AACCAGAAAA TAAGCTGGTA CTAGTTAAAC AATTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT CTGAGCTATG AGGCCGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TGAAACTTCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAATGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACTAAT
    TTTAGGGATA CAGTTGATAT ATTAGTCGGA TGGCATATAG ATCATACTCA GAAGCCTTCC
    CTCACACAGC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGATCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT CTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TGGCCTCCGG GGAGTCAGTG GATGAAGACG TTCCTCCTCC GTCAGTGTCC
    TTACCAAAGC TGGCAGCTCT CCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGGGG TCCTCCAATT ACAGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAGGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TCATTACATA TGGATTAGAT CAGCTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    TTGCCTTCAT CATTTGTAGA AAAACTATTT ATACCATCAT CTAAACTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGC
    CTGAAGAATA TTCCTGTTTT GGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ACCTGCTACA TAGTCTACAA CTTCCCGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATTT ATTCAACGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTCAGTGCTC TGACTACAAT TGGGAATGCT AAAAATTCAC TAATTGGGAT GTGGGCGCTG
    TCTCCAACCG TCTTTGCACT CCTGAGTAAG AATCTGATGA TTGTGCATGG TGACCTGGCC
    GTTCACTTCC CTGCCATTCA GTATGCAGTG CTCTACACGT TGTATTCACA TTGTACTAGG
    CATGACCACT TTATATCTAG TAGTCTGAGT TCTTCGTCCC CATCTTTGTT TGATGGAGCC
    GTGATTAGTA CTGTAACCAC AGCCACCAAA AAACATTTCT CAATTATATT AAATCTCTTG
    GGAATATTAC TAAAGAAGGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ACGCACCTTT ATTCTCTCTG
    CCATCTTTCC ATAAGTTTTC CAAAGGCCTT TTAGCCAACA CTCTTGTTGA AGATGTGAAT
    ATCTGTCTGC AAGCCTGCAG CAGTCTACAT GCTTTGTCCT CGTCTTTACC GGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAACTAGTCC ATAGTGGAAC TCGTATTCGC
    CAGGCATTTG GAAAGCTATT GAAGTCAATT CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AATACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGGAACTCT
    CATAGAACGG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ACAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACAAT TCCCCGCAAT CTTCTAAAGA CAGATGCTGT CCTTTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGTTGAGAAC TCCACTGGGC
    AGAGCTCAGG ATACCTTCCA GACAATTGAA GGTATCATCA GAAGTCTTGC AGCTCACACC
    TTAAACCCTG ATCAGGATGT TAGTCAGTGG ACAACTGCAG ACAATGATGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTTCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAGGGATG TGCCAATGCG TTGACATCAC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAACCGCCA GACTTGTCAA GACTGGCTGA CCCGAATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTAGC CGGCCAGCCT GCTGTGACAG TGAGACATGG CTTTGATTTG
    CTTACGGAAA TGAAGACAAA TCCATCTCAG GGGAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CACTGTGTGA ACTTCATTGT CCAGAAGCTA TACAAGGAAT TGCTGTCTGG
    TCATCTTCCA TCGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC CGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTG
    GATTGCTGCA TCTCCAGCTT TGACAAGTCT GTTCTCACAT TGGCTAATAC TGGGCGTAAC
    AGTGCCAGCC CCAAACATTC TTTGAACGGT GAATCCAGAA AAACTGTGCT GTCCAAACCA
    ACTGACTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    GCAATTGCCG ATTGGACTGC TGTACAGGAA TGGCAGAATG CTATCCATGA CTTGAAAAAG
    AGTACCAGCA GTACTTCTCT CAATCTAAAA GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TGTTGAATGT ACTGAGCAAT TGGAATTGTT ACCAGGAGAA
    AATATCAATC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTACTTCCT
    AATATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TCGAAGTTCA ATTGCTGAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CATATAGAAC AGGATCAGAA GTGGCAGTCT
    ATAACTGAAA ACGTGGTGAA GTACTTGAAG CAAACCTCCC GCATAGCTAT CGGGCCACTG
    AGACTTTCTA CTTTAACAGT TTCACAGTCT CTGCCAGTTC TAAGTACCTT GCAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTT CAACAGAGGA CTGTCTTATC
    CCACTCTTCA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAG
    GCATTAAGAT ACACCATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGACTGCTG GCACAGTGCA GTGAGGTTCA GCTGGCAAAG
    ACCACGACTG CGCAGGATTT AGTCCAGCAT TTTAAAAAGC TATCAACTCA AGGTCAAGTG
    GATGAAAAGT GGGGGCCTGA ACTTGACATT GAAAAAACCA AATTGCTATA TACAGCAGGC
    CAGTCAACAC ACGCAATGGA GATGCTGAGT TCTTGCGCCA TTTCGTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCCATT CTTACACTGG CCAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TATCAGGGCA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAAAACTTC
    ACAGGTCTTT CTACTTTATC TAAAAACATA CTCACTCTAA TTGAACTCCC ATCTGTTAAT
    ACAGTGGAAG AAGAGTATCC TCGAATCGAG AGCGAATCTA CAGTACATAT CGGAGTTGGA
    GAACCTGACT TCATTTTGGG ACAGTTGTAT CACCTATCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCAGCATT GGCTAGTTGG GCTTATAGAT GGGGCAGAAA GGTCGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCCAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACTATCAC TGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGGCAG
    GCAGTGTGTC GGCCAGCAGG AATTCAGGAT GAAGATATAA CGCTTCAGAT AACAGAAAGT
    GAAGATAATG AGGAAGATGA TATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCGAGCTGC
    CCCTGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGCG TTATTAAAGT GTGGAGGAAA
    GTTGTAGATA GAATCTTCAG CCTGTACAAA CTGTCTTGCA GCGCCTATTT TACTTTCCTT
    AAGCTCAATG CTGGTCAGAT TCCTTTAGAT GAAGATGACC CCAGGCTCCA TTTAAGTCAC
    AGACCGGAGC AGAGCACGGA TGACGTGATT GTGATGGCCA CATTGAGGCT GCTCAGACTG
    CTCGTGAAAC ATGCCGGCGA GCTCAGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCC
    ACTGCTCCCT GGAGAGGAAT TATTCCACAA CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AAAGTATTTG TAACCTTCTC TGCCGTGTTG CTCAGGATTC CCCCCATCTC
    ATATTGTACC CTGCGATAGT GGGCACCATA TCGCTTAGCA GTGAGTCACA GGCTTCAGGA
    AATAAGTTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAATTGCTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCCCAAG ATAGCAATAA GGATGAACCG
    AAGAGTGGAT TGAATGAAGA CCAAGCTATG ATGCAGGATT GCTACAGCAA GATTGTAGAT
    AAACTGTCCT CTGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTCGT GGCCGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGCTGGAA GACGAAGTGA AGAGAGTCCA GAATAACAAC
    ACCTTACGCA AAGAAGAGAA AATTGCAATC ATGCGAGAGA AGCACACAGC TTTGATGAAG
    CCCATTGTGT TTGCTTTGGA GCACGTGAGG AGTATCACGG CGGCCCCTGC AGAAACGCCT
    CATGAAAAGT GGTTTCAAGA TAACTATGGT GATGCAATTG AAAATGCCCT AGAAAAACTG
    AAGACACCAT CAAACCCTGC AAAGCCTGGA AGCAGTTGGA TTCCATTTAA AGAGATAATG
    CTAAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGCTACA TCTTGCGTCT TGAAGAAATC
    AGCCCGTGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCTGGAGA AGTCTCAGCC
    AGAGACACTG TCACAATCCA CAGTGTGGGC GGAACCATCA CAATCTTACC CACCAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCG GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ACTTACATCT GGATGAGAGA ATAATGCAGT TTTTATCTAT TGTAAATACT
    ATGTTTGCTA CAATCAATCG CCAGGAGACA CCCCGCTTCC ATGCGCGGCA CTATTCTGTA
    ACACCACTGG GAACTCGATC AGGACTGATC CAGTGGGTGG ATGGAGCCAC ACCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCTGCCTTAC AAGCACAAAA GGCCCAAGAT
    TCCTACCAGA CTCCTCAGAA TCCGGGAATT GTACCCCGTC CTAGTGAACT CTATTATAGT
    AAAATCGGTC CTGCTTTGAA AGCAGTGGGA CTTAGTCTGG ATGTGTCCCG ACGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGCTAGAA GAGTTGATGG AAGCCACGCC TCCAAATCTC
    CTTGCTAAAG AGCTGTGGTC ATCTTGTACC ACCCCTGATG AATGGTGGAG AGTCACACAG
    TCTTACGCCA GATCGACCGC GGTCATGTCT ATGGTCGGGT ACATAATTGG CCTTGGAGAC
    AGACATCTGG ATAACGTCCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTTCC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGACTTTCA
    TGTGAGCAGG TTTTGCACAT CATGCGGCGT GGCAGAGAGA CTCTGCTGAC ACTACTGGAG
    GCCTTTGTGT ACGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCCGTCTACG GTGGGGGAGG CCAGCAGGCC GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    AGAGAAATCA CCCGCAGCCT GTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAGAATAGGG ACGAGATGCT GGTTGTACTG CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT GACAGATGTG GAAAAACTGC AGGGCAAACT TCTGGAAGAA
    ATAGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAGCT ACAGACTCAG CAGAGAGCTG TTCAGGAGGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CATTATCAGG CTGCATTCAA TAATTTAGAA
    GCAACACAGC TGGCAAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT AGGTCCTCCA
    AGCTATGTGC CAGCAACAGC CTTTCTTCAG AACGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGT TAGAGGGGGA AGTTGGCGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTATGCA ACTGTAGCTC TGCAATATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAACTTAT CTGTAACACC
    ACTGTAGAGC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC ACCCCAGCCG
    CCCCCAACAG TGTGTCAGTT CATCACTGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCA
    GATATAAACA GTAGGCTTAT TAGACAGGTG GAACGATTGA AACAAGAAGC CGTCACCGTA
    CCTGTTTGTG AAGATCAGCT GAAAGAAATT GAACGTTGCA TCAAAGTTTT CCTTCATGAG
    AATGGAGAAG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TCTCTGCCCT TTGTACCCTT
    ACAAGGCGTA ACCTGATGAT GGAAGGGGCA GCTTCGAGTG CTGGAGAGCA GTTGGTGGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAGCTCT GCAGTATGAG TGGAAATGTC
    ACTTGCCTTG TTCAGTTACT GAAGCAGTGT CATCTGGTGC CACAGGACTT AGACATCCCT
    AACCCCATGG AGGCGTCTGA GGCAGTTCAC TTAGCTAATG GAGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGCGAATACA CATTAGAAAG TATGCTTCAT GAACTGGACA GCCTTATTGA GCAGACCACT
    GATGGCGTTC CTCTGCAGAC TCTAGTGGAA TCTCTTCAAG CCTATTTAAG AAACGCAGCC
    ATGGGACTTG AAGAAGAAAC ACATGCCCAT TACATCGATG TTGCCAGAAT ACTTCATGCT
    CAATATGGTG AGTTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCCTTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTAG TTGAAAAGCT AAACAAGATG GAAATTCCTA TCGCTTGGAG AAAGATTGAC
    ATTATAAGGG AAGCCAGGAG CACTCAAGTC AACTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATTTTCTT TCTAAAACGA CTGCAGACAA TTAAGGAGTT CTTCAGACTC
    TGCGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACCGATTGT CAACGTGAAA ACTCTTTTTA GGAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCTAT AAAGGCCTTC ACAGCTGACT TCGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACG CTGTGCAGCT TCATCAGTGC CCTAGGAGTG
    GACATCATTG CTCAAGTGGA GGCCAAGGAT TTTGGTGCTG AAAGCAAGGT TTCTGTTGAT
    GATCTGTGTA AGAAAGCAGT GGAGCACAAC ATCCAGGTCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT CGCCAGCTCC TATGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG TATCTCCTCC TGTAAGACGA GCCTGCAGCG CGTTCAGCTG
    CATATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAACAGACC ACAAGCGATG
    TCGGTCACAC CTCCCCCACG ATCTGCCATC CTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC TCTTGAAGCA
    AGTATTGAAC AGCGACTCAA GTGGGCAGGT GGTGCCAACC CTGCACTGGC ACCAGTACTA
    CAAGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAAGA GAGCCAAAGA
    GCCAGTCAGG TCACTTTCCT CTGCAGCAAT ATTATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCGGAAG CCTTAAACCT GGATGCTGCA TTATTTGAGC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCTTCACA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGAGTGG ACACAAGCCT TGAACACCCT ATTGGCAGCT CTGAATGGCT TTTATCAGCA
    CACAAACAGT TGACCCAGGA TATGTCTACG CAGAGAGCAA TTCAGACGGA GAAAGAGCAG
    CAGATAGAAA CGGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACAGGTCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGCGAAGAT GTTCCCTATG AGAACAGTGT GAGGCAGTTC
    TTGGGTGAAT ATAAATCTTG GCAAGACAAC ATTCAGACTG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAGGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACACA AAGTCAGAGT GTCTATAATA ATTTAGTGAG TTTTGCATCA
    CCACTAGTCA CCGATGCAAC AAATGAATGT TCAAGTCCAA CGTCATCTGC TACGTATCAG
    CCATCCTTTG CTGCAGCAGT CCGGAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAATG CTAGAAAGCT GATTCAGAAA AATCTAGCTA CATCAGCAGA TACTCCACCA
    AGCACTGTTC CAGGAACTGG CAAGAGTGCT GCTTGTAGTC CTAAAAAGGC CGTCAGAGAC
    CCTAAAACTG GGAAAGCTGT GCAAGAGAGA AATTCATATG CAGTGAGCGT GTGGAAGAGA
    GTGAAAGCCA AGTTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020942209.1
    CDS6093..17033
    Translation

    Target ORF information:

    RefSeq Version XM_021086550.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021086550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAT TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAACCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA CGATGATACC
    AGAATCCACG CTGACATACA AAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TCACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGACGA CAGAGACCGA AGACTGGCTA CTGTAAAGCA GTTGAAAGAG
    TTTATTCAGC AACCAGAAAA TAAGCTGGTA CTAGTTAAAC AATTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT CTGAGCTATG AGGCCGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TGAAACTTCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAATGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACTAAT
    TTTAGGGATA CAGTTGATAT ATTAGTCGGA TGGCATATAG ATCATACTCA GAAGCCTTCC
    CTCACACAGC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGATCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT CTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TGGCCTCCGG GGAGTCAGTG GATGAAGACG TTCCTCCTCC GTCAGTGTCC
    TTACCAAAGC TGGCAGCTCT CCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGGGG TCCTCCAATT ACAGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAGGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TCATTACATA TGGATTAGAT CAGCTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    TTGCCTTCAT CATTTGTAGA AAAACTATTT ATACCATCAT CTAAACTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGC
    CTGAAGAATA TTCCTGTTTT GGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ACCTGCTACA TAGTCTACAA CTTCCCGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATTT ATTCAACGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTCAGTGCTC TGACTACAAT TGGGAATGCT AAAAATTCAC TAATTGGGAT GTGGGCGCTG
    TCTCCAACCG TCTTTGCACT CCTGAGTAAG AATCTGATGA TTGTGCATGG TGACCTGGCC
    GTTCACTTCC CTGCCATTCA GTATGCAGTG CTCTACACGT TGTATTCACA TTGTACTAGG
    CATGACCACT TTATATCTAG TAGTCTGAGT TCTTCGTCCC CATCTTTGTT TGATGGAGCC
    GTGATTAGTA CTGTAACCAC AGCCACCAAA AAACATTTCT CAATTATATT AAATCTCTTG
    GGAATATTAC TAAAGAAGGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ACGCACCTTT ATTCTCTCTG
    CCATCTTTCC ATAAGTTTTC CAAAGGCCTT TTAGCCAACA CTCTTGTTGA AGATGTGAAT
    ATCTGTCTGC AAGCCTGCAG CAGTCTACAT GCTTTGTCCT CGTCTTTACC GGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAACTAGTCC ATAGTGGAAC TCGTATTCGC
    CAGGCATTTG GAAAGCTATT GAAGTCAATT CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AATACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGGAACTCT
    CATAGAACGG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ACAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACAAT TCCCCGCAAT CTTCTAAAGA CAGATGCTGT CCTTTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGTTGAGAAC TCCACTGGGC
    AGAGCTCAGG ATACCTTCCA GACAATTGAA GGTATCATCA GAAGTCTTGC AGCTCACACC
    TTAAACCCTG ATCAGGATGT TAGTCAGTGG ACAACTGCAG ACAATGATGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTTCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAGGGATG TGCCAATGCG TTGACATCAC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAACCGCCA GACTTGTCAA GACTGGCTGA CCCGAATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTAGC CGGCCAGCCT GCTGTGACAG TGAGACATGG CTTTGATTTG
    CTTACGGAAA TGAAGACAAA TCCATCTCAG GGGAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CACTGTGTGA ACTTCATTGT CCAGAAGCTA TACAAGGAAT TGCTGTCTGG
    TCATCTTCCA TCGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC CGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTG
    GATTGCTGCA TCTCCAGCTT TGACAAGTCT GTTCTCACAT TGGCTAATAC TGGGCGTAAC
    AGTGCCAGCC CCAAACATTC TTTGAACGGT GAATCCAGAA AAACTGTGCT GTCCAAACCA
    ACTGACTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    GCAATTGCCG ATTGGACTGC TGTACAGGAA TGGCAGAATG CTATCCATGA CTTGAAAAAG
    AGTACCAGCA GTACTTCTCT CAATCTAAAA GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TGTTGAATGT ACTGAGCAAT TGGAATTGTT ACCAGGAGAA
    AATATCAATC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTACTTCCT
    AATATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TCGAAGTTCA ATTGCTGAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CATATAGAAC AGGATCAGAA GTGGCAGTCT
    ATAACTGAAA ACGTGGTGAA GTACTTGAAG CAAACCTCCC GCATAGCTAT CGGGCCACTG
    AGACTTTCTA CTTTAACAGT TTCACAGTCT CTGCCAGTTC TAAGTACCTT GCAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTT CAACAGAGGA CTGTCTTATC
    CCACTCTTCA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAG
    GCATTAAGAT ACACCATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGACTGCTG GCACAGTGCA GTGAGGTTCA GCTGGCAAAG
    ACCACGACTG CGCAGGATTT AGTCCAGCAT TTTAAAAAGC TATCAACTCA AGGTCAAGTG
    GATGAAAAGT GGGGGCCTGA ACTTGACATT GAAAAAACCA AATTGCTATA TACAGCAGGC
    CAGTCAACAC ACGCAATGGA GATGCTGAGT TCTTGCGCCA TTTCGTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCCATT CTTACACTGG CCAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TATCAGGGCA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAAAACTTC
    ACAGGTCTTT CTACTTTATC TAAAAACATA CTCACTCTAA TTGAACTCCC ATCTGTTAAT
    ACAGTGGAAG AAGAGTATCC TCGAATCGAG AGCGAATCTA CAGTACATAT CGGAGTTGGA
    GAACCTGACT TCATTTTGGG ACAGTTGTAT CACCTATCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCAGCATT GGCTAGTTGG GCTTATAGAT GGGGCAGAAA GGTCGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCCAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACTATCAC TGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGGCAG
    GCAGTGTGTC GGCCAGCAGG AATTCAGGAT GAAGATATAA CGCTTCAGAT AACAGAAAGT
    GAAGATAATG AGGAAGATGA TATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCGAGCTGC
    CCCTGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGCG TTATTAAAGT GTGGAGGAAA
    GTTGTAGATA GAATCTTCAG CCTGTACAAA CTGTCTTGCA GCGCCTATTT TACTTTCCTT
    AAGCTCAATG CTGGTCAGAT TCCTTTAGAT GAAGATGACC CCAGGCTCCA TTTAAGTCAC
    AGACCGGAGC AGAGCACGGA TGACGTGATT GTGATGGCCA CATTGAGGCT GCTCAGACTG
    CTCGTGAAAC ATGCCGGCGA GCTCAGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCC
    ACTGCTCCCT GGAGAGGAAT TATTCCACAA CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AAAGTATTTG TAACCTTCTC TGCCGTGTTG CTCAGGATTC CCCCCATCTC
    ATATTGTACC CTGCGATAGT GGGCACCATA TCGCTTAGCA GTGAGTCACA GGCTTCAGGA
    AATAAGTTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAATTGCTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCCCAAG ATAGCAATAA GGATGAACCG
    AAGAGTGGAT TGAATGAAGA CCAAGCTATG ATGCAGGATT GCTACAGCAA GATTGTAGAT
    AAACTGTCCT CTGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTCGT GGCCGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGCTGGAA GACGAAGTGA AGAGAGTCCA GAATAACAAC
    ACCTTACGCA AAGAAGAGAA AATTGCAATC ATGCGAGAGA AGCACACAGC TTTGATGAAG
    CCCATTGTGT TTGCTTTGGA GCACGTGAGG AGTATCACGG CGGCCCCTGC AGAAACGCCT
    CATGAAAAGT GGTTTCAAGA TAACTATGGT GATGCAATTG AAAATGCCCT AGAAAAACTG
    AAGACACCAT CAAACCCTGC AAAGCCTGGA AGCAGTTGGA TTCCATTTAA AGAGATAATG
    CTAAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGCTACA TCTTGCGTCT TGAAGAAATC
    AGCCCGTGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCTGGAGA AGTCTCAGCC
    AGAGACACTG TCACAATCCA CAGTGTGGGC GGAACCATCA CAATCTTACC CACCAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCG GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ACTTACATCT GGATGAGAGA ATAATGCAGT TTTTATCTAT TGTAAATACT
    ATGTTTGCTA CAATCAATCG CCAGGAGACA CCCCGCTTCC ATGCGCGGCA CTATTCTGTA
    ACACCACTGG GAACTCGATC AGGACTGATC CAGTGGGTGG ATGGAGCCAC ACCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCTGCCTTAC AAGCACAAAA GGCCCAAGAT
    TCCTACCAGA CTCCTCAGAA TCCGGGAATT GTACCCCGTC CTAGTGAACT CTATTATAGT
    AAAATCGGTC CTGCTTTGAA AGCAGTGGGA CTTAGTCTGG ATGTGTCCCG ACGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGCTAGAA GAGTTGATGG AAGCCACGCC TCCAAATCTC
    CTTGCTAAAG AGCTGTGGTC ATCTTGTACC ACCCCTGATG AATGGTGGAG AGTCACACAG
    TCTTACGCCA GATCGACCGC GGTCATGTCT ATGGTCGGGT ACATAATTGG CCTTGGAGAC
    AGACATCTGG ATAACGTCCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTTCC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGACTTTCA
    TGTGAGCAGG TTTTGCACAT CATGCGGCGT GGCAGAGAGA CTCTGCTGAC ACTACTGGAG
    GCCTTTGTGT ACGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCCGTCTACG GTGGGGGAGG CCAGCAGGCC GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    AGAGAAATCA CCCGCAGCCT GTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAGAATAGGG ACGAGATGCT GGTTGTACTG CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT GACAGATGTG GAAAAACTGC AGGGCAAACT TCTGGAAGAA
    ATAGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAGCT ACAGACTCAG CAGAGAGCTG TTCAGGAGGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CATTATCAGG CTGCATTCAA TAATTTAGAA
    GCAACACAGC TGGCAAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT AGGTCCTCCA
    AGCTATGTGC CAGCAACAGC CTTTCTTCAG AACGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGT TAGAGGGGGA AGTTGGCGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTATGCA ACTGTAGCTC TGCAATATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAACTTAT CTGTAACACC
    ACTGTAGAGC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC ACCCCAGCCG
    CCCCCAACAG TGTGTCAGTT CATCACTGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCA
    GATATAAACA GTAGGCTTAT TAGACAGGTG GAACGATTGA AACAAGAAGC CGTCACCGTA
    CCTGTTTGTG AAGATCAGCT GAAAGAAATT GAACGTTGCA TCAAAGTTTT CCTTCATGAG
    AATGGAGAAG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TCTCTGCCCT TTGTACCCTT
    ACAAGGCGTA ACCTGATGAT GGAAGGGGCA GCTTCGAGTG CTGGAGAGCA GTTGGTGGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAGCTCT GCAGTATGAG TGGAAATGTC
    ACTTGCCTTG TTCAGTTACT GAAGCAGTGT CATCTGGTGC CACAGGACTT AGACATCCCT
    AACCCCATGG AGGCGTCTGA GGCAGTTCAC TTAGCTAATG GAGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGCGAATACA CATTAGAAAG TATGCTTCAT GAACTGGACA GCCTTATTGA GCAGACCACT
    GATGGCGTTC CTCTGCAGAC TCTAGTGGAA TCTCTTCAAG CCTATTTAAG AAACGCAGCC
    ATGGGACTTG AAGAAGAAAC ACATGCCCAT TACATCGATG TTGCCAGAAT ACTTCATGCT
    CAATATGGTG AGTTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCCTTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTAG TTGAAAAGCT AAACAAGATG GAAATTCCTA TCGCTTGGAG AAAGATTGAC
    ATTATAAGGG AAGCCAGGAG CACTCAAGTC AACTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATTTTCTT TCTAAAACGA CTGCAGACAA TTAAGGAGTT CTTCAGACTC
    TGCGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACCGATTGT CAACGTGAAA ACTCTTTTTA GGAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCTAT AAAGGCCTTC ACAGCTGACT TCGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACG CTGTGCAGCT TCATCAGTGC CCTAGGAGTG
    GACATCATTG CTCAAGTGGA GGCCAAGGAT TTTGGTGCTG AAAGCAAGGT TTCTGTTGAT
    GATCTGTGTA AGAAAGCAGT GGAGCACAAC ATCCAGGTCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT CGCCAGCTCC TATGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG TATCTCCTCC TGTAAGACGA GCCTGCAGCG CGTTCAGCTG
    CATATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAACAGACC ACAAGCGATG
    TCGGTCACAC CTCCCCCACG ATCTGCCATC CTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC TCTTGAAGCA
    AGTATTGAAC AGCGACTCAA GTGGGCAGGT GGTGCCAACC CTGCACTGGC ACCAGTACTA
    CAAGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAAGA GAGCCAAAGA
    GCCAGTCAGG TCACTTTCCT CTGCAGCAAT ATTATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCGGAAG CCTTAAACCT GGATGCTGCA TTATTTGAGC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCTTCACA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGAGTGG ACACAAGCCT TGAACACCCT ATTGGCAGCT CTGAATGGCT TTTATCAGCA
    CACAAACAGT TGACCCAGGA TATGTCTACG CAGAGAGCAA TTCAGACGGA GAAAGAGCAG
    CAGATAGAAA CGGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACAGGTCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGCGAAGAT GTTCCCTATG AGAACAGTGT GAGGCAGTTC
    TTGGGTGAAT ATAAATCTTG GCAAGACAAC ATTCAGACTG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAGGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACACA AAGTCAGAGT GTCTATAATA ATTTAGTGAG TTTTGCATCA
    CCACTAGTCA CCGATGCAAC AAATGAATGT TCAAGTCCAA CGTCATCTGC TACGTATCAG
    CCATCCTTTG CTGCAGCAGT CCGGAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAATG CTAGAAAGCT GATTCAGAAA AATCTAGCTA CATCAGCAGA TACTCCACCA
    AGCACTGTTC CAGGAACTGG CAAGAGTGCT GCTTGTAGTC CTAAAAAGGC CGTCAGAGAC
    CCTAAAACTG GGAAAGCTGT GCAAGAGAGA AATTCATATG CAGTGAGCGT GTGGAAGAGA
    GTGAAAGCCA AGTTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe245765
    Clone ID Related Accession (Same CDS sequence) XM_021086551.1
    Accession Version XM_021086551.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product serine/threonine-protein kinase SMG1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    ATGACTGGCA ACCCAGAGCG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
    CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAC TGGCTACTGT AAAGCAGTTG
    AAAGAGTTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC
    TTGGCTGCTG TACATGATGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG
    GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC CGAGAAGATC
    TTCAAATGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTGAA ACTTCTCTAC
    TTATGTGCCA CCTACAAAGC ACTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCTGTA
    ATGCAGCTTG TAATGACCAG CCTGCAGTCC ATTCTTGAAA ATGTGGATAC ACCAGAATTA
    CTTTGCAAAT GTGTTAAGTG CATTCTTTTG GTAGCTCGAT GTTACCCTCA TATTTTCAGC
    ACTAATTTTA GGGATACAGT TGATATATTA GTCGGATGGC ATATAGATCA TACTCAGAAG
    CCTTCCCTCA CACAGCAGGT GTCTGGGTGG TTGCAGAGTT TGGAGCCATT CTGGGTAGCT
    GATCTTGCAT TTTCTACCAC TCTTCTTGGT CAGTTTCTAG AGGACATGGA GGCATATGCT
    GAGGACCTCA GCCATGTGGC CTCCGGGGAG TCAGTGGATG AAGACGTTCC TCCTCCGTCA
    GTGTCCTTAC CAAAGCTGGC AGCTCTCCTC CGGGTATTCA GTACTGTGGT GAGAAGCATT
    GGGGAACGTT TCAGCCCAAT TCGGGGTCCT CCAATTACAG AGGCATATGT AACAGATGTT
    CTGTACAGAG TAATGAGATG TGTGACGGCT GCAAACCAGG TGTTTTTTTC TGAGGCTGTG
    TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT
    ATACATTGTG ACATGGTCAT TACATATGGA TTAGATCAGC TGGAGAATTG CCAGACTTGT
    GGTACAGATT ATATCATCTC AGTCTTGAAT TTACTCACAT TGATTGTTGA ACAGATAAAT
    ACAAAATTGC CTTCATCATT TGTAGAAAAA CTATTTATAC CATCATCTAA ACTACTATTT
    TTGCGTTATC ATAAAGAAAA AGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG
    CTCAGCCTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AATTAATTTT GGGAGAAATG
    ACTTGTGCCC TAAACAACCT GCTACATAGT CTACAACTTC CCGATGCCTG TTCTGAAATA
    AAACATGAGG CTTTTAAGAA TCATTTATTC AACGTAGACA ATGCAAAATT TGTAGTTATA
    TTTGACCTCA GTGCTCTGAC TACAATTGGG AATGCTAAAA ATTCACTAAT TGGGATGTGG
    GCGCTGTCTC CAACCGTCTT TGCACTCCTG AGTAAGAATC TGATGATTGT GCATGGTGAC
    CTGGCCGTTC ACTTCCCTGC CATTCAGTAT GCAGTGCTCT ACACGTTGTA TTCACATTGT
    ACTAGGCATG ACCACTTTAT ATCTAGTAGT CTGAGTTCTT CGTCCCCATC TTTGTTTGAT
    GGAGCCGTGA TTAGTACTGT AACCACAGCC ACCAAAAAAC ATTTCTCAAT TATATTAAAT
    CTCTTGGGAA TATTACTAAA GAAGGATAAT CTTAACCAGG ACACAAGGAA ACTGTTAATG
    ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATACGC ACCTTTATTC
    TCTCTGCCAT CTTTCCATAA GTTTTCCAAA GGCCTTTTAG CCAACACTCT TGTTGAAGAT
    GTGAATATCT GTCTGCAAGC CTGCAGCAGT CTACATGCTT TGTCCTCGTC TTTACCGGAT
    GATCTTTTAC AGAGGTGTGT TGATGTTTGC CGTGTTCAAC TAGTCCATAG TGGAACTCGT
    ATTCGCCAGG CATTTGGAAA GCTATTGAAG TCAATTCCTT TAGATGTTGT CTTGAGCAAT
    AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA
    CCAAGTAATA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG
    AACTCTCATA GAACGGGGAA GGACAATTGG TTAGAAAGAT TGTTCTACAG CTGCCAGAGA
    CTGGATAAAC GTGACCAATC AACAATTCCC CGCAATCTTC TAAAGACAGA TGCTGTCCTT
    TGGCAGTGGG CTATTTGGGA AGCAGCACAG TTCACTGTCC TTTCTAAGTT GAGAACTCCA
    CTGGGCAGAG CTCAGGATAC CTTCCAGACA ATTGAAGGTA TCATCAGAAG TCTTGCAGCT
    CACACCTTAA ACCCTGATCA GGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC
    CACAGTAGCA ACCAGCTTAG ACTTGTGCTT CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG
    TTAATGTATA ATGCATATGA GGGATGTGCC AATGCGTTGA CATCACCTCC CAAGGTCATT
    AGGACTTTTT TCTATACCAA CCGCCAGACT TGTCAAGACT GGCTGACCCG AATCCGACTC
    TCCATCATGA GGGTTGGATT GTTAGCCGGC CAGCCTGCTG TGACAGTGAG ACATGGCTTT
    GATTTGCTTA CGGAAATGAA GACAAATCCA TCTCAGGGGA ATGAATTGGA AGTAACTATC
    ATGATGGTGG TAGAAGCACT GTGTGAACTT CATTGTCCAG AAGCTATACA AGGAATTGCT
    GTCTGGTCAT CTTCCATCGT TGGAAAAAAT CTTCTCTGGA TTAATTCAGT GGCCCAACAG
    GCTGAAGGGA GGTTTGAAAA GGCCTCCGTG GAGTACCAGG AGCACCTGTG TGCCATGACA
    GGTGTGGATT GCTGCATCTC CAGCTTTGAC AAGTCTGTTC TCACATTGGC TAATACTGGG
    CGTAACAGTG CCAGCCCCAA ACATTCTTTG AACGGTGAAT CCAGAAAAAC TGTGCTGTCC
    AAACCAACTG ACTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC
    TACATTGCAA TTGCCGATTG GACTGCTGTA CAGGAATGGC AGAATGCTAT CCATGACTTG
    AAAAAGAGTA CCAGCAGTAC TTCTCTCAAT CTAAAAGCTG ATTTCAACTA CATAAAATCA
    CTAAGCAGTT TTGAATCTGG AGAATTTGTT GAATGTACTG AGCAATTGGA ATTGTTACCA
    GGAGAAAATA TCAATCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTA
    CTTCCTAATA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATCGA AGTTCAATTG
    CTGAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCATA TAGAACAGGA TCAGAAGTGG
    CAGTCTATAA CTGAAAACGT GGTGAAGTAC TTGAAGCAAA CCTCCCGCAT AGCTATCGGG
    CCACTGAGAC TTTCTACTTT AACAGTTTCA CAGTCTCTGC CAGTTCTAAG TACCTTGCAG
    CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTTCAAC AGAGGACTGT
    CTTATCCCAC TCTTCAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG
    ATGCAGGCAT TAAGATACAC CATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA
    ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA
    AAACGGGGGA ATGTGTCACT TGCAACAAGA CTGCTGGCAC AGTGCAGTGA GGTTCAGCTG
    GCAAAGACCA CGACTGCGCA GGATTTAGTC CAGCATTTTA AAAAGCTATC AACTCAAGGT
    CAAGTGGATG AAAAGTGGGG GCCTGAACTT GACATTGAAA AAACCAAATT GCTATATACA
    GCAGGCCAGT CAACACACGC AATGGAGATG CTGAGTTCTT GCGCCATTTC GTTCTGCAAG
    TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCCATTCTTA CACTGGCCAA ATGGATCCAG
    GCAGAATGGA AAGAAATATC AGGGCAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAA
    AACTTCACAG GTCTTTCTAC TTTATCTAAA AACATACTCA CTCTAATTGA ACTCCCATCT
    GTTAATACAG TGGAAGAAGA GTATCCTCGA ATCGAGAGCG AATCTACAGT ACATATCGGA
    GTTGGAGAAC CTGACTTCAT TTTGGGACAG TTGTATCACC TATCTTCAGT ACAGGCACCT
    GAAGTAGCCA AATCTTGGGC AGCATTGGCT AGTTGGGCTT ATAGATGGGG CAGAAAGGTC
    GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CCAGAGAAAA ATCTGAAGTT
    CAGAATCTGC TCCCAGACAC TATCACTGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT
    GGGCAGGCAG TGTGTCGGCC AGCAGGAATT CAGGATGAAG ATATAACGCT TCAGATAACA
    GAAAGTGAAG ATAATGAGGA AGATGATATG GTTGATGTTA TCTGGCGTCA GTTAATATCG
    AGCTGCCCCT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGCGTTAT TAAAGTGTGG
    AGGAAAGTTG TAGATAGAAT CTTCAGCCTG TACAAACTGT CTTGCAGCGC CTATTTTACT
    TTCCTTAAGC TCAATGCTGG TCAGATTCCT TTAGATGAAG ATGACCCCAG GCTCCATTTA
    AGTCACAGAC CGGAGCAGAG CACGGATGAC GTGATTGTGA TGGCCACATT GAGGCTGCTC
    AGACTGCTCG TGAAACATGC CGGCGAGCTC AGGCAGTATC TGGAACATGG CTTGGAAACA
    ACACCCACTG CTCCCTGGAG AGGAATTATT CCACAACTTT TCTCACGCTT AAACCACCCT
    GAAGTGTATG TGCGCCAAAG TATTTGTAAC CTTCTCTGCC GTGTTGCTCA GGATTCCCCC
    CATCTCATAT TGTACCCTGC GATAGTGGGC ACCATATCGC TTAGCAGTGA GTCACAGGCT
    TCAGGAAATA AGTTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA
    TTGCTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CCCAAGATAG CAATAAGGAT
    GAACCGAAGA GTGGATTGAA TGAAGACCAA GCTATGATGC AGGATTGCTA CAGCAAGATT
    GTAGATAAAC TGTCCTCTGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTCGTGGCC
    GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG
    CACATGTATG TCCTGAGACG AATTCAGCAG CTGGAAGACG AAGTGAAGAG AGTCCAGAAT
    AACAACACCT TACGCAAAGA AGAGAAAATT GCAATCATGC GAGAGAAGCA CACAGCTTTG
    ATGAAGCCCA TTGTGTTTGC TTTGGAGCAC GTGAGGAGTA TCACGGCGGC CCCTGCAGAA
    ACGCCTCATG AAAAGTGGTT TCAAGATAAC TATGGTGATG CAATTGAAAA TGCCCTAGAA
    AAACTGAAGA CACCATCAAA CCCTGCAAAG CCTGGAAGCA GTTGGATTCC ATTTAAAGAG
    ATAATGCTAA GTTTGCAACA GAGAGCACAG AAACGTGCAA GCTACATCTT GCGTCTTGAA
    GAAATCAGCC CGTGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC TGGAGAAGTC
    TCAGCCAGAG ACACTGTCAC AATCCACAGT GTGGGCGGAA CCATCACAAT CTTACCCACC
    AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCGGATG GGAAGAGCTA TCCTTATCTT
    TTCAAAGGAT TGGAGGACTT ACATCTGGAT GAGAGAATAA TGCAGTTTTT ATCTATTGTA
    AATACTATGT TTGCTACAAT CAATCGCCAG GAGACACCCC GCTTCCATGC GCGGCACTAT
    TCTGTAACAC CACTGGGAAC TCGATCAGGA CTGATCCAGT GGGTGGATGG AGCCACACCC
    TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCTG CCTTACAAGC ACAAAAGGCC
    CAAGATTCCT ACCAGACTCC TCAGAATCCG GGAATTGTAC CCCGTCCTAG TGAACTCTAT
    TATAGTAAAA TCGGTCCTGC TTTGAAAGCA GTGGGACTTA GTCTGGATGT GTCCCGACGG
    GATTGGCCTC TTCATGTGAT GAAGGCAGTG CTAGAAGAGT TGATGGAAGC CACGCCTCCA
    AATCTCCTTG CTAAAGAGCT GTGGTCATCT TGTACCACCC CTGATGAATG GTGGAGAGTC
    ACACAGTCTT ACGCCAGATC GACCGCGGTC ATGTCTATGG TCGGGTACAT AATTGGCCTT
    GGAGACAGAC ATCTGGATAA CGTCCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA
    GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTTCCTTTT
    CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGA
    CTTTCATGTG AGCAGGTTTT GCACATCATG CGGCGTGGCA GAGAGACTCT GCTGACACTA
    CTGGAGGCCT TTGTGTACGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT
    GCTGGTGCCG TCTACGGTGG GGGAGGCCAG CAGGCCGAGA GCAAGCAGAG CAAGAGAGAG
    ATGGAAAGAG AAATCACCCG CAGCCTGTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC
    TGGTTTAAGA ATAGGGACGA GATGCTGGTT GTACTGCCCA AGCTGGACAG CAGCTTAGAT
    GAATACCTGA GCTTGCAAGA GCAACTGACA GATGTGGAAA AACTGCAGGG CAAACTTCTG
    GAAGAAATAG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA
    CACAGGTATT CTGAACACAC TCAGCTACAG ACTCAGCAGA GAGCTGTTCA GGAGGCAATC
    CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACATT ATCAGGCTGC ATTCAATAAT
    TTAGAAGCAA CACAGCTGGC AAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTAGGT
    CCTCCAAGCT ATGTGCCAGC AACAGCCTTT CTTCAGAACG CTGGCCAGGC CCACTTGATT
    AGCCAGTGTG AGCAGTTAGA GGGGGAAGTT GGCGCCCTCC TGCAGCAGAG GCGCTCGGTG
    CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TATGCAACTG TAGCTCTGCA ATATCCAAAG
    GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA ACTTATCTGT
    AACACCACTG TAGAGCGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCACCC
    CAGCCGCCCC CAACAGTGTG TCAGTTCATC ACTGCCACTG AGATGACCCT GCAGCGGTAC
    GCAGCAGATA TAAACAGTAG GCTTATTAGA CAGGTGGAAC GATTGAAACA AGAAGCCGTC
    ACCGTACCTG TTTGTGAAGA TCAGCTGAAA GAAATTGAAC GTTGCATCAA AGTTTTCCTT
    CATGAGAATG GAGAAGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATCTC TGCCCTTTGT
    ACCCTTACAA GGCGTAACCT GATGATGGAA GGGGCAGCTT CGAGTGCTGG AGAGCAGTTG
    GTGGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AGCTCTGCAG TATGAGTGGA
    AATGTCACTT GCCTTGTTCA GTTACTGAAG CAGTGTCATC TGGTGCCACA GGACTTAGAC
    ATCCCTAACC CCATGGAGGC GTCTGAGGCA GTTCACTTAG CTAATGGAGT ATACACCTCA
    CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA
    ATGAAAGGCG AATACACATT AGAAAGTATG CTTCATGAAC TGGACAGCCT TATTGAGCAG
    ACCACTGATG GCGTTCCTCT GCAGACTCTA GTGGAATCTC TTCAAGCCTA TTTAAGAAAC
    GCAGCCATGG GACTTGAAGA AGAAACACAT GCCCATTACA TCGATGTTGC CAGAATACTT
    CATGCTCAAT ATGGTGAGTT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA
    ATGTCAGCTG GCCAGATGCT TTTGGTAGCC TTTGATGGCA TGTTTGCCCA AGTTGAGACT
    GCTTTTGGCT TATTAGTTGA AAAGCTAAAC AAGATGGAAA TTCCTATCGC TTGGAGAAAG
    ATTGACATTA TAAGGGAAGC CAGGAGCACT CAAGTCAACT TTTTTGATGA TGATAATCAC
    CGACAGGTGC TGGAAGAGAT TTTCTTTCTA AAACGACTGC AGACAATTAA GGAGTTCTTC
    AGACTCTGCG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT
    ACTGTAAATG GGCCTGTACC GATTGTCAAC GTGAAAACTC TTTTTAGGAA CTCTTGTTTC
    AGTGAAGACC AGATGGCCAA ACCTATAAAG GCCTTCACAG CTGACTTCGT GCGGCAGCTC
    CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACGCTGT GCAGCTTCAT CAGTGCCCTA
    GGAGTGGACA TCATTGCTCA AGTGGAGGCC AAGGATTTTG GTGCTGAAAG CAAGGTTTCT
    GTTGATGATC TGTGTAAGAA AGCAGTGGAG CACAACATCC AGGTCGGGAA GTTCTCTCAG
    CTGGTCATGA ACAGGGCGAC TGTGCTCGCC AGCTCCTATG ACACTGCCTG GAAGAAGCAC
    GACTTGGTGC GCAGGCTGGA GACCAGTATC TCCTCCTGTA AGACGAGCCT GCAGCGCGTT
    CAGCTGCATA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA CAGACCACAA
    GCGATGTCGG TCACACCTCC CCCACGATCT GCCATCCTAA CCAGCATGAA AAAGAAACTC
    CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCTCTT
    GAAGCAAGTA TTGAACAGCG ACTCAAGTGG GCAGGTGGTG CCAACCCTGC ACTGGCACCA
    GTACTACAAG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAAGAGAGC
    CAAAGAGCCA GTCAGGTCAC TTTCCTCTGC AGCAATATTA TTCATTTTGA AAGTTTACGA
    ACTAGAACTG CGGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAGCTAAT CAAACGGTGT
    CAGCAAATGT GTTCATTTGC TTCACAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT
    TTATTACAGA GAGTGGACAC AAGCCTTGAA CACCCTATTG GCAGCTCTGA ATGGCTTTTA
    TCAGCACACA AACAGTTGAC CCAGGATATG TCTACGCAGA GAGCAATTCA GACGGAGAAA
    GAGCAGCAGA TAGAAACGGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT
    GTGCTCACAG GTCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC
    AAGGATGAAG AAGCTGCTCT GGCAGATGGC GAAGATGTTC CCTATGAGAA CAGTGTGAGG
    CAGTTCTTGG GTGAATATAA ATCTTGGCAA GACAACATTC AGACTGTGCT GTTTACATTA
    GTCCAAGCCA TGGGTCAGGT TCGAAGTCAG GAACATGTTG AAATGCTCCA GGAAATAACT
    CCCACCTTGA AAGAACTGAA AACACAAAGT CAGAGTGTCT ATAATAATTT AGTGAGTTTT
    GCATCACCAC TAGTCACCGA TGCAACAAAT GAATGTTCAA GTCCAACGTC ATCTGCTACG
    TATCAGCCAT CCTTTGCTGC AGCAGTCCGG AGCAACACTG GCCAGAAGAC TCAGCCTGAT
    GTCATGTCAC AGAATGCTAG AAAGCTGATT CAGAAAAATC TAGCTACATC AGCAGATACT
    CCACCAAGCA CTGTTCCAGG AACTGGCAAG AGTGCTGCTT GTAGTCCTAA AAAGGCCGTC
    AGAGACCCTA AAACTGGGAA AGCTGTGCAA GAGAGAAATT CATATGCAGT GAGCGTGTGG
    AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT
    GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG
    TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020942210.1
    CDS426..11012
    Translation

    Target ORF information:

    RefSeq Version XM_021086551.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021086551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    ATGACTGGCA ACCCAGAGCG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
    CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAC TGGCTACTGT AAAGCAGTTG
    AAAGAGTTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC
    TTGGCTGCTG TACATGATGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG
    GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC CGAGAAGATC
    TTCAAATGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTGAA ACTTCTCTAC
    TTATGTGCCA CCTACAAAGC ACTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCTGTA
    ATGCAGCTTG TAATGACCAG CCTGCAGTCC ATTCTTGAAA ATGTGGATAC ACCAGAATTA
    CTTTGCAAAT GTGTTAAGTG CATTCTTTTG GTAGCTCGAT GTTACCCTCA TATTTTCAGC
    ACTAATTTTA GGGATACAGT TGATATATTA GTCGGATGGC ATATAGATCA TACTCAGAAG
    CCTTCCCTCA CACAGCAGGT GTCTGGGTGG TTGCAGAGTT TGGAGCCATT CTGGGTAGCT
    GATCTTGCAT TTTCTACCAC TCTTCTTGGT CAGTTTCTAG AGGACATGGA GGCATATGCT
    GAGGACCTCA GCCATGTGGC CTCCGGGGAG TCAGTGGATG AAGACGTTCC TCCTCCGTCA
    GTGTCCTTAC CAAAGCTGGC AGCTCTCCTC CGGGTATTCA GTACTGTGGT GAGAAGCATT
    GGGGAACGTT TCAGCCCAAT TCGGGGTCCT CCAATTACAG AGGCATATGT AACAGATGTT
    CTGTACAGAG TAATGAGATG TGTGACGGCT GCAAACCAGG TGTTTTTTTC TGAGGCTGTG
    TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT
    ATACATTGTG ACATGGTCAT TACATATGGA TTAGATCAGC TGGAGAATTG CCAGACTTGT
    GGTACAGATT ATATCATCTC AGTCTTGAAT TTACTCACAT TGATTGTTGA ACAGATAAAT
    ACAAAATTGC CTTCATCATT TGTAGAAAAA CTATTTATAC CATCATCTAA ACTACTATTT
    TTGCGTTATC ATAAAGAAAA AGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG
    CTCAGCCTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AATTAATTTT GGGAGAAATG
    ACTTGTGCCC TAAACAACCT GCTACATAGT CTACAACTTC CCGATGCCTG TTCTGAAATA
    AAACATGAGG CTTTTAAGAA TCATTTATTC AACGTAGACA ATGCAAAATT TGTAGTTATA
    TTTGACCTCA GTGCTCTGAC TACAATTGGG AATGCTAAAA ATTCACTAAT TGGGATGTGG
    GCGCTGTCTC CAACCGTCTT TGCACTCCTG AGTAAGAATC TGATGATTGT GCATGGTGAC
    CTGGCCGTTC ACTTCCCTGC CATTCAGTAT GCAGTGCTCT ACACGTTGTA TTCACATTGT
    ACTAGGCATG ACCACTTTAT ATCTAGTAGT CTGAGTTCTT CGTCCCCATC TTTGTTTGAT
    GGAGCCGTGA TTAGTACTGT AACCACAGCC ACCAAAAAAC ATTTCTCAAT TATATTAAAT
    CTCTTGGGAA TATTACTAAA GAAGGATAAT CTTAACCAGG ACACAAGGAA ACTGTTAATG
    ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATACGC ACCTTTATTC
    TCTCTGCCAT CTTTCCATAA GTTTTCCAAA GGCCTTTTAG CCAACACTCT TGTTGAAGAT
    GTGAATATCT GTCTGCAAGC CTGCAGCAGT CTACATGCTT TGTCCTCGTC TTTACCGGAT
    GATCTTTTAC AGAGGTGTGT TGATGTTTGC CGTGTTCAAC TAGTCCATAG TGGAACTCGT
    ATTCGCCAGG CATTTGGAAA GCTATTGAAG TCAATTCCTT TAGATGTTGT CTTGAGCAAT
    AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA
    CCAAGTAATA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG
    AACTCTCATA GAACGGGGAA GGACAATTGG TTAGAAAGAT TGTTCTACAG CTGCCAGAGA
    CTGGATAAAC GTGACCAATC AACAATTCCC CGCAATCTTC TAAAGACAGA TGCTGTCCTT
    TGGCAGTGGG CTATTTGGGA AGCAGCACAG TTCACTGTCC TTTCTAAGTT GAGAACTCCA
    CTGGGCAGAG CTCAGGATAC CTTCCAGACA ATTGAAGGTA TCATCAGAAG TCTTGCAGCT
    CACACCTTAA ACCCTGATCA GGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC
    CACAGTAGCA ACCAGCTTAG ACTTGTGCTT CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG
    TTAATGTATA ATGCATATGA GGGATGTGCC AATGCGTTGA CATCACCTCC CAAGGTCATT
    AGGACTTTTT TCTATACCAA CCGCCAGACT TGTCAAGACT GGCTGACCCG AATCCGACTC
    TCCATCATGA GGGTTGGATT GTTAGCCGGC CAGCCTGCTG TGACAGTGAG ACATGGCTTT
    GATTTGCTTA CGGAAATGAA GACAAATCCA TCTCAGGGGA ATGAATTGGA AGTAACTATC
    ATGATGGTGG TAGAAGCACT GTGTGAACTT CATTGTCCAG AAGCTATACA AGGAATTGCT
    GTCTGGTCAT CTTCCATCGT TGGAAAAAAT CTTCTCTGGA TTAATTCAGT GGCCCAACAG
    GCTGAAGGGA GGTTTGAAAA GGCCTCCGTG GAGTACCAGG AGCACCTGTG TGCCATGACA
    GGTGTGGATT GCTGCATCTC CAGCTTTGAC AAGTCTGTTC TCACATTGGC TAATACTGGG
    CGTAACAGTG CCAGCCCCAA ACATTCTTTG AACGGTGAAT CCAGAAAAAC TGTGCTGTCC
    AAACCAACTG ACTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC
    TACATTGCAA TTGCCGATTG GACTGCTGTA CAGGAATGGC AGAATGCTAT CCATGACTTG
    AAAAAGAGTA CCAGCAGTAC TTCTCTCAAT CTAAAAGCTG ATTTCAACTA CATAAAATCA
    CTAAGCAGTT TTGAATCTGG AGAATTTGTT GAATGTACTG AGCAATTGGA ATTGTTACCA
    GGAGAAAATA TCAATCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTA
    CTTCCTAATA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATCGA AGTTCAATTG
    CTGAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCATA TAGAACAGGA TCAGAAGTGG
    CAGTCTATAA CTGAAAACGT GGTGAAGTAC TTGAAGCAAA CCTCCCGCAT AGCTATCGGG
    CCACTGAGAC TTTCTACTTT AACAGTTTCA CAGTCTCTGC CAGTTCTAAG TACCTTGCAG
    CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTTCAAC AGAGGACTGT
    CTTATCCCAC TCTTCAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG
    ATGCAGGCAT TAAGATACAC CATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA
    ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA
    AAACGGGGGA ATGTGTCACT TGCAACAAGA CTGCTGGCAC AGTGCAGTGA GGTTCAGCTG
    GCAAAGACCA CGACTGCGCA GGATTTAGTC CAGCATTTTA AAAAGCTATC AACTCAAGGT
    CAAGTGGATG AAAAGTGGGG GCCTGAACTT GACATTGAAA AAACCAAATT GCTATATACA
    GCAGGCCAGT CAACACACGC AATGGAGATG CTGAGTTCTT GCGCCATTTC GTTCTGCAAG
    TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCCATTCTTA CACTGGCCAA ATGGATCCAG
    GCAGAATGGA AAGAAATATC AGGGCAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAA
    AACTTCACAG GTCTTTCTAC TTTATCTAAA AACATACTCA CTCTAATTGA ACTCCCATCT
    GTTAATACAG TGGAAGAAGA GTATCCTCGA ATCGAGAGCG AATCTACAGT ACATATCGGA
    GTTGGAGAAC CTGACTTCAT TTTGGGACAG TTGTATCACC TATCTTCAGT ACAGGCACCT
    GAAGTAGCCA AATCTTGGGC AGCATTGGCT AGTTGGGCTT ATAGATGGGG CAGAAAGGTC
    GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CCAGAGAAAA ATCTGAAGTT
    CAGAATCTGC TCCCAGACAC TATCACTGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT
    GGGCAGGCAG TGTGTCGGCC AGCAGGAATT CAGGATGAAG ATATAACGCT TCAGATAACA
    GAAAGTGAAG ATAATGAGGA AGATGATATG GTTGATGTTA TCTGGCGTCA GTTAATATCG
    AGCTGCCCCT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGCGTTAT TAAAGTGTGG
    AGGAAAGTTG TAGATAGAAT CTTCAGCCTG TACAAACTGT CTTGCAGCGC CTATTTTACT
    TTCCTTAAGC TCAATGCTGG TCAGATTCCT TTAGATGAAG ATGACCCCAG GCTCCATTTA
    AGTCACAGAC CGGAGCAGAG CACGGATGAC GTGATTGTGA TGGCCACATT GAGGCTGCTC
    AGACTGCTCG TGAAACATGC CGGCGAGCTC AGGCAGTATC TGGAACATGG CTTGGAAACA
    ACACCCACTG CTCCCTGGAG AGGAATTATT CCACAACTTT TCTCACGCTT AAACCACCCT
    GAAGTGTATG TGCGCCAAAG TATTTGTAAC CTTCTCTGCC GTGTTGCTCA GGATTCCCCC
    CATCTCATAT TGTACCCTGC GATAGTGGGC ACCATATCGC TTAGCAGTGA GTCACAGGCT
    TCAGGAAATA AGTTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA
    TTGCTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CCCAAGATAG CAATAAGGAT
    GAACCGAAGA GTGGATTGAA TGAAGACCAA GCTATGATGC AGGATTGCTA CAGCAAGATT
    GTAGATAAAC TGTCCTCTGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTCGTGGCC
    GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG
    CACATGTATG TCCTGAGACG AATTCAGCAG CTGGAAGACG AAGTGAAGAG AGTCCAGAAT
    AACAACACCT TACGCAAAGA AGAGAAAATT GCAATCATGC GAGAGAAGCA CACAGCTTTG
    ATGAAGCCCA TTGTGTTTGC TTTGGAGCAC GTGAGGAGTA TCACGGCGGC CCCTGCAGAA
    ACGCCTCATG AAAAGTGGTT TCAAGATAAC TATGGTGATG CAATTGAAAA TGCCCTAGAA
    AAACTGAAGA CACCATCAAA CCCTGCAAAG CCTGGAAGCA GTTGGATTCC ATTTAAAGAG
    ATAATGCTAA GTTTGCAACA GAGAGCACAG AAACGTGCAA GCTACATCTT GCGTCTTGAA
    GAAATCAGCC CGTGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC TGGAGAAGTC
    TCAGCCAGAG ACACTGTCAC AATCCACAGT GTGGGCGGAA CCATCACAAT CTTACCCACC
    AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCGGATG GGAAGAGCTA TCCTTATCTT
    TTCAAAGGAT TGGAGGACTT ACATCTGGAT GAGAGAATAA TGCAGTTTTT ATCTATTGTA
    AATACTATGT TTGCTACAAT CAATCGCCAG GAGACACCCC GCTTCCATGC GCGGCACTAT
    TCTGTAACAC CACTGGGAAC TCGATCAGGA CTGATCCAGT GGGTGGATGG AGCCACACCC
    TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCTG CCTTACAAGC ACAAAAGGCC
    CAAGATTCCT ACCAGACTCC TCAGAATCCG GGAATTGTAC CCCGTCCTAG TGAACTCTAT
    TATAGTAAAA TCGGTCCTGC TTTGAAAGCA GTGGGACTTA GTCTGGATGT GTCCCGACGG
    GATTGGCCTC TTCATGTGAT GAAGGCAGTG CTAGAAGAGT TGATGGAAGC CACGCCTCCA
    AATCTCCTTG CTAAAGAGCT GTGGTCATCT TGTACCACCC CTGATGAATG GTGGAGAGTC
    ACACAGTCTT ACGCCAGATC GACCGCGGTC ATGTCTATGG TCGGGTACAT AATTGGCCTT
    GGAGACAGAC ATCTGGATAA CGTCCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA
    GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTTCCTTTT
    CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGA
    CTTTCATGTG AGCAGGTTTT GCACATCATG CGGCGTGGCA GAGAGACTCT GCTGACACTA
    CTGGAGGCCT TTGTGTACGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT
    GCTGGTGCCG TCTACGGTGG GGGAGGCCAG CAGGCCGAGA GCAAGCAGAG CAAGAGAGAG
    ATGGAAAGAG AAATCACCCG CAGCCTGTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC
    TGGTTTAAGA ATAGGGACGA GATGCTGGTT GTACTGCCCA AGCTGGACAG CAGCTTAGAT
    GAATACCTGA GCTTGCAAGA GCAACTGACA GATGTGGAAA AACTGCAGGG CAAACTTCTG
    GAAGAAATAG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA
    CACAGGTATT CTGAACACAC TCAGCTACAG ACTCAGCAGA GAGCTGTTCA GGAGGCAATC
    CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACATT ATCAGGCTGC ATTCAATAAT
    TTAGAAGCAA CACAGCTGGC AAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTAGGT
    CCTCCAAGCT ATGTGCCAGC AACAGCCTTT CTTCAGAACG CTGGCCAGGC CCACTTGATT
    AGCCAGTGTG AGCAGTTAGA GGGGGAAGTT GGCGCCCTCC TGCAGCAGAG GCGCTCGGTG
    CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TATGCAACTG TAGCTCTGCA ATATCCAAAG
    GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA ACTTATCTGT
    AACACCACTG TAGAGCGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCACCC
    CAGCCGCCCC CAACAGTGTG TCAGTTCATC ACTGCCACTG AGATGACCCT GCAGCGGTAC
    GCAGCAGATA TAAACAGTAG GCTTATTAGA CAGGTGGAAC GATTGAAACA AGAAGCCGTC
    ACCGTACCTG TTTGTGAAGA TCAGCTGAAA GAAATTGAAC GTTGCATCAA AGTTTTCCTT
    CATGAGAATG GAGAAGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATCTC TGCCCTTTGT
    ACCCTTACAA GGCGTAACCT GATGATGGAA GGGGCAGCTT CGAGTGCTGG AGAGCAGTTG
    GTGGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AGCTCTGCAG TATGAGTGGA
    AATGTCACTT GCCTTGTTCA GTTACTGAAG CAGTGTCATC TGGTGCCACA GGACTTAGAC
    ATCCCTAACC CCATGGAGGC GTCTGAGGCA GTTCACTTAG CTAATGGAGT ATACACCTCA
    CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA
    ATGAAAGGCG AATACACATT AGAAAGTATG CTTCATGAAC TGGACAGCCT TATTGAGCAG
    ACCACTGATG GCGTTCCTCT GCAGACTCTA GTGGAATCTC TTCAAGCCTA TTTAAGAAAC
    GCAGCCATGG GACTTGAAGA AGAAACACAT GCCCATTACA TCGATGTTGC CAGAATACTT
    CATGCTCAAT ATGGTGAGTT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA
    ATGTCAGCTG GCCAGATGCT TTTGGTAGCC TTTGATGGCA TGTTTGCCCA AGTTGAGACT
    GCTTTTGGCT TATTAGTTGA AAAGCTAAAC AAGATGGAAA TTCCTATCGC TTGGAGAAAG
    ATTGACATTA TAAGGGAAGC CAGGAGCACT CAAGTCAACT TTTTTGATGA TGATAATCAC
    CGACAGGTGC TGGAAGAGAT TTTCTTTCTA AAACGACTGC AGACAATTAA GGAGTTCTTC
    AGACTCTGCG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT
    ACTGTAAATG GGCCTGTACC GATTGTCAAC GTGAAAACTC TTTTTAGGAA CTCTTGTTTC
    AGTGAAGACC AGATGGCCAA ACCTATAAAG GCCTTCACAG CTGACTTCGT GCGGCAGCTC
    CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACGCTGT GCAGCTTCAT CAGTGCCCTA
    GGAGTGGACA TCATTGCTCA AGTGGAGGCC AAGGATTTTG GTGCTGAAAG CAAGGTTTCT
    GTTGATGATC TGTGTAAGAA AGCAGTGGAG CACAACATCC AGGTCGGGAA GTTCTCTCAG
    CTGGTCATGA ACAGGGCGAC TGTGCTCGCC AGCTCCTATG ACACTGCCTG GAAGAAGCAC
    GACTTGGTGC GCAGGCTGGA GACCAGTATC TCCTCCTGTA AGACGAGCCT GCAGCGCGTT
    CAGCTGCATA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA CAGACCACAA
    GCGATGTCGG TCACACCTCC CCCACGATCT GCCATCCTAA CCAGCATGAA AAAGAAACTC
    CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCTCTT
    GAAGCAAGTA TTGAACAGCG ACTCAAGTGG GCAGGTGGTG CCAACCCTGC ACTGGCACCA
    GTACTACAAG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAAGAGAGC
    CAAAGAGCCA GTCAGGTCAC TTTCCTCTGC AGCAATATTA TTCATTTTGA AAGTTTACGA
    ACTAGAACTG CGGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAGCTAAT CAAACGGTGT
    CAGCAAATGT GTTCATTTGC TTCACAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT
    TTATTACAGA GAGTGGACAC AAGCCTTGAA CACCCTATTG GCAGCTCTGA ATGGCTTTTA
    TCAGCACACA AACAGTTGAC CCAGGATATG TCTACGCAGA GAGCAATTCA GACGGAGAAA
    GAGCAGCAGA TAGAAACGGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT
    GTGCTCACAG GTCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC
    AAGGATGAAG AAGCTGCTCT GGCAGATGGC GAAGATGTTC CCTATGAGAA CAGTGTGAGG
    CAGTTCTTGG GTGAATATAA ATCTTGGCAA GACAACATTC AGACTGTGCT GTTTACATTA
    GTCCAAGCCA TGGGTCAGGT TCGAAGTCAG GAACATGTTG AAATGCTCCA GGAAATAACT
    CCCACCTTGA AAGAACTGAA AACACAAAGT CAGAGTGTCT ATAATAATTT AGTGAGTTTT
    GCATCACCAC TAGTCACCGA TGCAACAAAT GAATGTTCAA GTCCAACGTC ATCTGCTACG
    TATCAGCCAT CCTTTGCTGC AGCAGTCCGG AGCAACACTG GCCAGAAGAC TCAGCCTGAT
    GTCATGTCAC AGAATGCTAG AAAGCTGATT CAGAAAAATC TAGCTACATC AGCAGATACT
    CCACCAAGCA CTGTTCCAGG AACTGGCAAG AGTGCTGCTT GTAGTCCTAA AAAGGCCGTC
    AGAGACCCTA AAACTGGGAA AGCTGTGCAA GAGAGAAATT CATATGCAGT GAGCGTGTGG
    AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT
    GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG
    TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe245766
    Clone ID Related Accession (Same CDS sequence) XM_021086552.1
    Accession Version XM_021086552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product serine/threonine-protein kinase SMG1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGACTATAC ATTGTGACAT GGTCATTACA TATGGATTAG ATCAGCTGGA GAATTGCCAG 
    ACTTGTGGTA CAGATTATAT CATCTCAGTC TTGAATTTAC TCACATTGAT TGTTGAACAG
    ATAAATACAA AATTGCCTTC ATCATTTGTA GAAAAACTAT TTATACCATC ATCTAAACTA
    CTATTTTTGC GTTATCATAA AGAAAAAGAG GTTGTTGCTG TAGCCCATGC TGTTTATCAA
    GCAGTGCTCA GCCTGAAGAA TATTCCTGTT TTGGAGACTG CCTATAAATT AATTTTGGGA
    GAAATGACTT GTGCCCTAAA CAACCTGCTA CATAGTCTAC AACTTCCCGA TGCCTGTTCT
    GAAATAAAAC ATGAGGCTTT TAAGAATCAT TTATTCAACG TAGACAATGC AAAATTTGTA
    GTTATATTTG ACCTCAGTGC TCTGACTACA ATTGGGAATG CTAAAAATTC ACTAATTGGG
    ATGTGGGCGC TGTCTCCAAC CGTCTTTGCA CTCCTGAGTA AGAATCTGAT GATTGTGCAT
    GGTGACCTGG CCGTTCACTT CCCTGCCATT CAGTATGCAG TGCTCTACAC GTTGTATTCA
    CATTGTACTA GGCATGACCA CTTTATATCT AGTAGTCTGA GTTCTTCGTC CCCATCTTTG
    TTTGATGGAG CCGTGATTAG TACTGTAACC ACAGCCACCA AAAAACATTT CTCAATTATA
    TTAAATCTCT TGGGAATATT ACTAAAGAAG GATAATCTTA ACCAGGACAC AAGGAAACTG
    TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATACGCACCT
    TTATTCTCTC TGCCATCTTT CCATAAGTTT TCCAAAGGCC TTTTAGCCAA CACTCTTGTT
    GAAGATGTGA ATATCTGTCT GCAAGCCTGC AGCAGTCTAC ATGCTTTGTC CTCGTCTTTA
    CCGGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAACTAGT CCATAGTGGA
    ACTCGTATTC GCCAGGCATT TGGAAAGCTA TTGAAGTCAA TTCCTTTAGA TGTTGTCTTG
    AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT
    AAAGCACCAA GTAATACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG
    TATGGGAACT CTCATAGAAC GGGGAAGGAC AATTGGTTAG AAAGATTGTT CTACAGCTGC
    CAGAGACTGG ATAAACGTGA CCAATCAACA ATTCCCCGCA ATCTTCTAAA GACAGATGCT
    GTCCTTTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGTTGAGA
    ACTCCACTGG GCAGAGCTCA GGATACCTTC CAGACAATTG AAGGTATCAT CAGAAGTCTT
    GCAGCTCACA CCTTAAACCC TGATCAGGAT GTTAGTCAGT GGACAACTGC AGACAATGAT
    GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTTCTTC TGCAGTATCT GGAAAACCTG
    GAGAAGTTAA TGTATAATGC ATATGAGGGA TGTGCCAATG CGTTGACATC ACCTCCCAAG
    GTCATTAGGA CTTTTTTCTA TACCAACCGC CAGACTTGTC AAGACTGGCT GACCCGAATC
    CGACTCTCCA TCATGAGGGT TGGATTGTTA GCCGGCCAGC CTGCTGTGAC AGTGAGACAT
    GGCTTTGATT TGCTTACGGA AATGAAGACA AATCCATCTC AGGGGAATGA ATTGGAAGTA
    ACTATCATGA TGGTGGTAGA AGCACTGTGT GAACTTCATT GTCCAGAAGC TATACAAGGA
    ATTGCTGTCT GGTCATCTTC CATCGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC
    CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCCGTGGAGT ACCAGGAGCA CCTGTGTGCC
    ATGACAGGTG TGGATTGCTG CATCTCCAGC TTTGACAAGT CTGTTCTCAC ATTGGCTAAT
    ACTGGGCGTA ACAGTGCCAG CCCCAAACAT TCTTTGAACG GTGAATCCAG AAAAACTGTG
    CTGTCCAAAC CAACTGACTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT
    GAGTGCTACA TTGCAATTGC CGATTGGACT GCTGTACAGG AATGGCAGAA TGCTATCCAT
    GACTTGAAAA AGAGTACCAG CAGTACTTCT CTCAATCTAA AAGCTGATTT CAACTACATA
    AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTGTTGAAT GTACTGAGCA ATTGGAATTG
    TTACCAGGAG AAAATATCAA TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA
    AAACTACTTC CTAATATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATCGAAGTT
    CAATTGCTGA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCATATAGA ACAGGATCAG
    AAGTGGCAGT CTATAACTGA AAACGTGGTG AAGTACTTGA AGCAAACCTC CCGCATAGCT
    ATCGGGCCAC TGAGACTTTC TACTTTAACA GTTTCACAGT CTCTGCCAGT TCTAAGTACC
    TTGCAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TTCAACAGAG
    GACTGTCTTA TCCCACTCTT CAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG
    CCATGGATGC AGGCATTAAG ATACACCATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA
    GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT
    GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGACTGC TGGCACAGTG CAGTGAGGTT
    CAGCTGGCAA AGACCACGAC TGCGCAGGAT TTAGTCCAGC ATTTTAAAAA GCTATCAACT
    CAAGGTCAAG TGGATGAAAA GTGGGGGCCT GAACTTGACA TTGAAAAAAC CAAATTGCTA
    TATACAGCAG GCCAGTCAAC ACACGCAATG GAGATGCTGA GTTCTTGCGC CATTTCGTTC
    TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCCA TTCTTACACT GGCCAAATGG
    ATCCAGGCAG AATGGAAAGA AATATCAGGG CAGCTGAAAC AGGTTTACAG AGCTCAGCAG
    CAGCAAAACT TCACAGGTCT TTCTACTTTA TCTAAAAACA TACTCACTCT AATTGAACTC
    CCATCTGTTA ATACAGTGGA AGAAGAGTAT CCTCGAATCG AGAGCGAATC TACAGTACAT
    ATCGGAGTTG GAGAACCTGA CTTCATTTTG GGACAGTTGT ATCACCTATC TTCAGTACAG
    GCACCTGAAG TAGCCAAATC TTGGGCAGCA TTGGCTAGTT GGGCTTATAG ATGGGGCAGA
    AAGGTCGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCCAG AGAAAAATCT
    GAAGTTCAGA ATCTGCTCCC AGACACTATC ACTGAAGAAG AAAAAGAGAG AATATATGGT
    ATTCTTGGGC AGGCAGTGTG TCGGCCAGCA GGAATTCAGG ATGAAGATAT AACGCTTCAG
    ATAACAGAAA GTGAAGATAA TGAGGAAGAT GATATGGTTG ATGTTATCTG GCGTCAGTTA
    ATATCGAGCT GCCCCTGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG CGTTATTAAA
    GTGTGGAGGA AAGTTGTAGA TAGAATCTTC AGCCTGTACA AACTGTCTTG CAGCGCCTAT
    TTTACTTTCC TTAAGCTCAA TGCTGGTCAG ATTCCTTTAG ATGAAGATGA CCCCAGGCTC
    CATTTAAGTC ACAGACCGGA GCAGAGCACG GATGACGTGA TTGTGATGGC CACATTGAGG
    CTGCTCAGAC TGCTCGTGAA ACATGCCGGC GAGCTCAGGC AGTATCTGGA ACATGGCTTG
    GAAACAACAC CCACTGCTCC CTGGAGAGGA ATTATTCCAC AACTTTTCTC ACGCTTAAAC
    CACCCTGAAG TGTATGTGCG CCAAAGTATT TGTAACCTTC TCTGCCGTGT TGCTCAGGAT
    TCCCCCCATC TCATATTGTA CCCTGCGATA GTGGGCACCA TATCGCTTAG CAGTGAGTCA
    CAGGCTTCAG GAAATAAGTT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA
    GAAGAATTGC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCCCA AGATAGCAAT
    AAGGATGAAC CGAAGAGTGG ATTGAATGAA GACCAAGCTA TGATGCAGGA TTGCTACAGC
    AAGATTGTAG ATAAACTGTC CTCTGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTC
    GTGGCCGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA
    CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGCTGG AAGACGAAGT GAAGAGAGTC
    CAGAATAACA ACACCTTACG CAAAGAAGAG AAAATTGCAA TCATGCGAGA GAAGCACACA
    GCTTTGATGA AGCCCATTGT GTTTGCTTTG GAGCACGTGA GGAGTATCAC GGCGGCCCCT
    GCAGAAACGC CTCATGAAAA GTGGTTTCAA GATAACTATG GTGATGCAAT TGAAAATGCC
    CTAGAAAAAC TGAAGACACC ATCAAACCCT GCAAAGCCTG GAAGCAGTTG GATTCCATTT
    AAAGAGATAA TGCTAAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGCTA CATCTTGCGT
    CTTGAAGAAA TCAGCCCGTG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCTGGA
    GAAGTCTCAG CCAGAGACAC TGTCACAATC CACAGTGTGG GCGGAACCAT CACAATCTTA
    CCCACCAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CGGATGGGAA GAGCTATCCT
    TATCTTTTCA AAGGATTGGA GGACTTACAT CTGGATGAGA GAATAATGCA GTTTTTATCT
    ATTGTAAATA CTATGTTTGC TACAATCAAT CGCCAGGAGA CACCCCGCTT CCATGCGCGG
    CACTATTCTG TAACACCACT GGGAACTCGA TCAGGACTGA TCCAGTGGGT GGATGGAGCC
    ACACCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCTGCCTT ACAAGCACAA
    AAGGCCCAAG ATTCCTACCA GACTCCTCAG AATCCGGGAA TTGTACCCCG TCCTAGTGAA
    CTCTATTATA GTAAAATCGG TCCTGCTTTG AAAGCAGTGG GACTTAGTCT GGATGTGTCC
    CGACGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGCTAG AAGAGTTGAT GGAAGCCACG
    CCTCCAAATC TCCTTGCTAA AGAGCTGTGG TCATCTTGTA CCACCCCTGA TGAATGGTGG
    AGAGTCACAC AGTCTTACGC CAGATCGACC GCGGTCATGT CTATGGTCGG GTACATAATT
    GGCCTTGGAG ACAGACATCT GGATAACGTC CTCATAGATA TGACGACTGG AGAGGTTGTT
    CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTT
    CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT
    TTTAGACTTT CATGTGAGCA GGTTTTGCAC ATCATGCGGC GTGGCAGAGA GACTCTGCTG
    ACACTACTGG AGGCCTTTGT GTACGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT
    GGGTTTGCTG GTGCCGTCTA CGGTGGGGGA GGCCAGCAGG CCGAGAGCAA GCAGAGCAAG
    AGAGAGATGG AAAGAGAAAT CACCCGCAGC CTGTTTTCTT CGAGAGTAGC TGAAATTAAG
    GTGAACTGGT TTAAGAATAG GGACGAGATG CTGGTTGTAC TGCCCAAGCT GGACAGCAGC
    TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTGACAGATG TGGAAAAACT GCAGGGCAAA
    CTTCTGGAAG AAATAGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT
    CTGCAACACA GGTATTCTGA ACACACTCAG CTACAGACTC AGCAGAGAGC TGTTCAGGAG
    GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACATTATCA GGCTGCATTC
    AATAATTTAG AAGCAACACA GCTGGCAAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC
    CTAGGTCCTC CAAGCTATGT GCCAGCAACA GCCTTTCTTC AGAACGCTGG CCAGGCCCAC
    TTGATTAGCC AGTGTGAGCA GTTAGAGGGG GAAGTTGGCG CCCTCCTGCA GCAGAGGCGC
    TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTATG CAACTGTAGC TCTGCAATAT
    CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAACTT
    ATCTGTAACA CCACTGTAGA GCGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT
    GCACCCCAGC CGCCCCCAAC AGTGTGTCAG TTCATCACTG CCACTGAGAT GACCCTGCAG
    CGGTACGCAG CAGATATAAA CAGTAGGCTT ATTAGACAGG TGGAACGATT GAAACAAGAA
    GCCGTCACCG TACCTGTTTG TGAAGATCAG CTGAAAGAAA TTGAACGTTG CATCAAAGTT
    TTCCTTCATG AGAATGGAGA AGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATCTCTGCC
    CTTTGTACCC TTACAAGGCG TAACCTGATG ATGGAAGGGG CAGCTTCGAG TGCTGGAGAG
    CAGTTGGTGG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAGCT CTGCAGTATG
    AGTGGAAATG TCACTTGCCT TGTTCAGTTA CTGAAGCAGT GTCATCTGGT GCCACAGGAC
    TTAGACATCC CTAACCCCAT GGAGGCGTCT GAGGCAGTTC ACTTAGCTAA TGGAGTATAC
    ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA
    TGTTTAATGA AAGGCGAATA CACATTAGAA AGTATGCTTC ATGAACTGGA CAGCCTTATT
    GAGCAGACCA CTGATGGCGT TCCTCTGCAG ACTCTAGTGG AATCTCTTCA AGCCTATTTA
    AGAAACGCAG CCATGGGACT TGAAGAAGAA ACACATGCCC ATTACATCGA TGTTGCCAGA
    ATACTTCATG CTCAATATGG TGAGTTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA
    CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCCTTTG ATGGCATGTT TGCCCAAGTT
    GAGACTGCTT TTGGCTTATT AGTTGAAAAG CTAAACAAGA TGGAAATTCC TATCGCTTGG
    AGAAAGATTG ACATTATAAG GGAAGCCAGG AGCACTCAAG TCAACTTTTT TGATGATGAT
    AATCACCGAC AGGTGCTGGA AGAGATTTTC TTTCTAAAAC GACTGCAGAC AATTAAGGAG
    TTCTTCAGAC TCTGCGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT
    CAGAATACTG TAAATGGGCC TGTACCGATT GTCAACGTGA AAACTCTTTT TAGGAACTCT
    TGTTTCAGTG AAGACCAGAT GGCCAAACCT ATAAAGGCCT TCACAGCTGA CTTCGTGCGG
    CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CGCTGTGCAG CTTCATCAGT
    GCCCTAGGAG TGGACATCAT TGCTCAAGTG GAGGCCAAGG ATTTTGGTGC TGAAAGCAAG
    GTTTCTGTTG ATGATCTGTG TAAGAAAGCA GTGGAGCACA ACATCCAGGT CGGGAAGTTC
    TCTCAGCTGG TCATGAACAG GGCGACTGTG CTCGCCAGCT CCTATGACAC TGCCTGGAAG
    AAGCACGACT TGGTGCGCAG GCTGGAGACC AGTATCTCCT CCTGTAAGAC GAGCCTGCAG
    CGCGTTCAGC TGCATATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAACAGA
    CCACAAGCGA TGTCGGTCAC ACCTCCCCCA CGATCTGCCA TCCTAACCAG CATGAAAAAG
    AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA
    GCTCTTGAAG CAAGTATTGA ACAGCGACTC AAGTGGGCAG GTGGTGCCAA CCCTGCACTG
    GCACCAGTAC TACAAGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAA
    GAGAGCCAAA GAGCCAGTCA GGTCACTTTC CTCTGCAGCA ATATTATTCA TTTTGAAAGT
    TTACGAACTA GAACTGCGGA AGCCTTAAAC CTGGATGCTG CATTATTTGA GCTAATCAAA
    CGGTGTCAGC AAATGTGTTC ATTTGCTTCA CAGTTTAACA GTTCAGTCTC TGAGTTAGAG
    CTTCGTTTAT TACAGAGAGT GGACACAAGC CTTGAACACC CTATTGGCAG CTCTGAATGG
    CTTTTATCAG CACACAAACA GTTGACCCAG GATATGTCTA CGCAGAGAGC AATTCAGACG
    GAGAAAGAGC AGCAGATAGA AACGGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA
    AAGACTGTGC TCACAGGTCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA
    ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGCGAAG ATGTTCCCTA TGAGAACAGT
    GTGAGGCAGT TCTTGGGTGA ATATAAATCT TGGCAAGACA ACATTCAGAC TGTGCTGTTT
    ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAGGAAC ATGTTGAAAT GCTCCAGGAA
    ATAACTCCCA CCTTGAAAGA ACTGAAAACA CAAAGTCAGA GTGTCTATAA TAATTTAGTG
    AGTTTTGCAT CACCACTAGT CACCGATGCA ACAAATGAAT GTTCAAGTCC AACGTCATCT
    GCTACGTATC AGCCATCCTT TGCTGCAGCA GTCCGGAGCA ACACTGGCCA GAAGACTCAG
    CCTGATGTCA TGTCACAGAA TGCTAGAAAG CTGATTCAGA AAAATCTAGC TACATCAGCA
    GATACTCCAC CAAGCACTGT TCCAGGAACT GGCAAGAGTG CTGCTTGTAG TCCTAAAAAG
    GCCGTCAGAG ACCCTAAAAC TGGGAAAGCT GTGCAAGAGA GAAATTCATA TGCAGTGAGC
    GTGTGGAAGA GAGTGAAAGC CAAGTTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG
    TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT
    CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020942211.1
    CDS1714..11286
    Translation

    Target ORF information:

    RefSeq Version XM_021086552.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021086552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGACTATAC ATTGTGACAT GGTCATTACA TATGGATTAG ATCAGCTGGA GAATTGCCAG 
    ACTTGTGGTA CAGATTATAT CATCTCAGTC TTGAATTTAC TCACATTGAT TGTTGAACAG
    ATAAATACAA AATTGCCTTC ATCATTTGTA GAAAAACTAT TTATACCATC ATCTAAACTA
    CTATTTTTGC GTTATCATAA AGAAAAAGAG GTTGTTGCTG TAGCCCATGC TGTTTATCAA
    GCAGTGCTCA GCCTGAAGAA TATTCCTGTT TTGGAGACTG CCTATAAATT AATTTTGGGA
    GAAATGACTT GTGCCCTAAA CAACCTGCTA CATAGTCTAC AACTTCCCGA TGCCTGTTCT
    GAAATAAAAC ATGAGGCTTT TAAGAATCAT TTATTCAACG TAGACAATGC AAAATTTGTA
    GTTATATTTG ACCTCAGTGC TCTGACTACA ATTGGGAATG CTAAAAATTC ACTAATTGGG
    ATGTGGGCGC TGTCTCCAAC CGTCTTTGCA CTCCTGAGTA AGAATCTGAT GATTGTGCAT
    GGTGACCTGG CCGTTCACTT CCCTGCCATT CAGTATGCAG TGCTCTACAC GTTGTATTCA
    CATTGTACTA GGCATGACCA CTTTATATCT AGTAGTCTGA GTTCTTCGTC CCCATCTTTG
    TTTGATGGAG CCGTGATTAG TACTGTAACC ACAGCCACCA AAAAACATTT CTCAATTATA
    TTAAATCTCT TGGGAATATT ACTAAAGAAG GATAATCTTA ACCAGGACAC AAGGAAACTG
    TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATACGCACCT
    TTATTCTCTC TGCCATCTTT CCATAAGTTT TCCAAAGGCC TTTTAGCCAA CACTCTTGTT
    GAAGATGTGA ATATCTGTCT GCAAGCCTGC AGCAGTCTAC ATGCTTTGTC CTCGTCTTTA
    CCGGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAACTAGT CCATAGTGGA
    ACTCGTATTC GCCAGGCATT TGGAAAGCTA TTGAAGTCAA TTCCTTTAGA TGTTGTCTTG
    AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT
    AAAGCACCAA GTAATACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG
    TATGGGAACT CTCATAGAAC GGGGAAGGAC AATTGGTTAG AAAGATTGTT CTACAGCTGC
    CAGAGACTGG ATAAACGTGA CCAATCAACA ATTCCCCGCA ATCTTCTAAA GACAGATGCT
    GTCCTTTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGTTGAGA
    ACTCCACTGG GCAGAGCTCA GGATACCTTC CAGACAATTG AAGGTATCAT CAGAAGTCTT
    GCAGCTCACA CCTTAAACCC TGATCAGGAT GTTAGTCAGT GGACAACTGC AGACAATGAT
    GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTTCTTC TGCAGTATCT GGAAAACCTG
    GAGAAGTTAA TGTATAATGC ATATGAGGGA TGTGCCAATG CGTTGACATC ACCTCCCAAG
    GTCATTAGGA CTTTTTTCTA TACCAACCGC CAGACTTGTC AAGACTGGCT GACCCGAATC
    CGACTCTCCA TCATGAGGGT TGGATTGTTA GCCGGCCAGC CTGCTGTGAC AGTGAGACAT
    GGCTTTGATT TGCTTACGGA AATGAAGACA AATCCATCTC AGGGGAATGA ATTGGAAGTA
    ACTATCATGA TGGTGGTAGA AGCACTGTGT GAACTTCATT GTCCAGAAGC TATACAAGGA
    ATTGCTGTCT GGTCATCTTC CATCGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC
    CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCCGTGGAGT ACCAGGAGCA CCTGTGTGCC
    ATGACAGGTG TGGATTGCTG CATCTCCAGC TTTGACAAGT CTGTTCTCAC ATTGGCTAAT
    ACTGGGCGTA ACAGTGCCAG CCCCAAACAT TCTTTGAACG GTGAATCCAG AAAAACTGTG
    CTGTCCAAAC CAACTGACTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT
    GAGTGCTACA TTGCAATTGC CGATTGGACT GCTGTACAGG AATGGCAGAA TGCTATCCAT
    GACTTGAAAA AGAGTACCAG CAGTACTTCT CTCAATCTAA AAGCTGATTT CAACTACATA
    AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTGTTGAAT GTACTGAGCA ATTGGAATTG
    TTACCAGGAG AAAATATCAA TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA
    AAACTACTTC CTAATATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATCGAAGTT
    CAATTGCTGA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCATATAGA ACAGGATCAG
    AAGTGGCAGT CTATAACTGA AAACGTGGTG AAGTACTTGA AGCAAACCTC CCGCATAGCT
    ATCGGGCCAC TGAGACTTTC TACTTTAACA GTTTCACAGT CTCTGCCAGT TCTAAGTACC
    TTGCAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TTCAACAGAG
    GACTGTCTTA TCCCACTCTT CAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG
    CCATGGATGC AGGCATTAAG ATACACCATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA
    GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT
    GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGACTGC TGGCACAGTG CAGTGAGGTT
    CAGCTGGCAA AGACCACGAC TGCGCAGGAT TTAGTCCAGC ATTTTAAAAA GCTATCAACT
    CAAGGTCAAG TGGATGAAAA GTGGGGGCCT GAACTTGACA TTGAAAAAAC CAAATTGCTA
    TATACAGCAG GCCAGTCAAC ACACGCAATG GAGATGCTGA GTTCTTGCGC CATTTCGTTC
    TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCCA TTCTTACACT GGCCAAATGG
    ATCCAGGCAG AATGGAAAGA AATATCAGGG CAGCTGAAAC AGGTTTACAG AGCTCAGCAG
    CAGCAAAACT TCACAGGTCT TTCTACTTTA TCTAAAAACA TACTCACTCT AATTGAACTC
    CCATCTGTTA ATACAGTGGA AGAAGAGTAT CCTCGAATCG AGAGCGAATC TACAGTACAT
    ATCGGAGTTG GAGAACCTGA CTTCATTTTG GGACAGTTGT ATCACCTATC TTCAGTACAG
    GCACCTGAAG TAGCCAAATC TTGGGCAGCA TTGGCTAGTT GGGCTTATAG ATGGGGCAGA
    AAGGTCGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCCAG AGAAAAATCT
    GAAGTTCAGA ATCTGCTCCC AGACACTATC ACTGAAGAAG AAAAAGAGAG AATATATGGT
    ATTCTTGGGC AGGCAGTGTG TCGGCCAGCA GGAATTCAGG ATGAAGATAT AACGCTTCAG
    ATAACAGAAA GTGAAGATAA TGAGGAAGAT GATATGGTTG ATGTTATCTG GCGTCAGTTA
    ATATCGAGCT GCCCCTGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG CGTTATTAAA
    GTGTGGAGGA AAGTTGTAGA TAGAATCTTC AGCCTGTACA AACTGTCTTG CAGCGCCTAT
    TTTACTTTCC TTAAGCTCAA TGCTGGTCAG ATTCCTTTAG ATGAAGATGA CCCCAGGCTC
    CATTTAAGTC ACAGACCGGA GCAGAGCACG GATGACGTGA TTGTGATGGC CACATTGAGG
    CTGCTCAGAC TGCTCGTGAA ACATGCCGGC GAGCTCAGGC AGTATCTGGA ACATGGCTTG
    GAAACAACAC CCACTGCTCC CTGGAGAGGA ATTATTCCAC AACTTTTCTC ACGCTTAAAC
    CACCCTGAAG TGTATGTGCG CCAAAGTATT TGTAACCTTC TCTGCCGTGT TGCTCAGGAT
    TCCCCCCATC TCATATTGTA CCCTGCGATA GTGGGCACCA TATCGCTTAG CAGTGAGTCA
    CAGGCTTCAG GAAATAAGTT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA
    GAAGAATTGC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCCCA AGATAGCAAT
    AAGGATGAAC CGAAGAGTGG ATTGAATGAA GACCAAGCTA TGATGCAGGA TTGCTACAGC
    AAGATTGTAG ATAAACTGTC CTCTGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTC
    GTGGCCGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA
    CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGCTGG AAGACGAAGT GAAGAGAGTC
    CAGAATAACA ACACCTTACG CAAAGAAGAG AAAATTGCAA TCATGCGAGA GAAGCACACA
    GCTTTGATGA AGCCCATTGT GTTTGCTTTG GAGCACGTGA GGAGTATCAC GGCGGCCCCT
    GCAGAAACGC CTCATGAAAA GTGGTTTCAA GATAACTATG GTGATGCAAT TGAAAATGCC
    CTAGAAAAAC TGAAGACACC ATCAAACCCT GCAAAGCCTG GAAGCAGTTG GATTCCATTT
    AAAGAGATAA TGCTAAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGCTA CATCTTGCGT
    CTTGAAGAAA TCAGCCCGTG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCTGGA
    GAAGTCTCAG CCAGAGACAC TGTCACAATC CACAGTGTGG GCGGAACCAT CACAATCTTA
    CCCACCAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CGGATGGGAA GAGCTATCCT
    TATCTTTTCA AAGGATTGGA GGACTTACAT CTGGATGAGA GAATAATGCA GTTTTTATCT
    ATTGTAAATA CTATGTTTGC TACAATCAAT CGCCAGGAGA CACCCCGCTT CCATGCGCGG
    CACTATTCTG TAACACCACT GGGAACTCGA TCAGGACTGA TCCAGTGGGT GGATGGAGCC
    ACACCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCTGCCTT ACAAGCACAA
    AAGGCCCAAG ATTCCTACCA GACTCCTCAG AATCCGGGAA TTGTACCCCG TCCTAGTGAA
    CTCTATTATA GTAAAATCGG TCCTGCTTTG AAAGCAGTGG GACTTAGTCT GGATGTGTCC
    CGACGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGCTAG AAGAGTTGAT GGAAGCCACG
    CCTCCAAATC TCCTTGCTAA AGAGCTGTGG TCATCTTGTA CCACCCCTGA TGAATGGTGG
    AGAGTCACAC AGTCTTACGC CAGATCGACC GCGGTCATGT CTATGGTCGG GTACATAATT
    GGCCTTGGAG ACAGACATCT GGATAACGTC CTCATAGATA TGACGACTGG AGAGGTTGTT
    CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTT
    CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT
    TTTAGACTTT CATGTGAGCA GGTTTTGCAC ATCATGCGGC GTGGCAGAGA GACTCTGCTG
    ACACTACTGG AGGCCTTTGT GTACGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT
    GGGTTTGCTG GTGCCGTCTA CGGTGGGGGA GGCCAGCAGG CCGAGAGCAA GCAGAGCAAG
    AGAGAGATGG AAAGAGAAAT CACCCGCAGC CTGTTTTCTT CGAGAGTAGC TGAAATTAAG
    GTGAACTGGT TTAAGAATAG GGACGAGATG CTGGTTGTAC TGCCCAAGCT GGACAGCAGC
    TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTGACAGATG TGGAAAAACT GCAGGGCAAA
    CTTCTGGAAG AAATAGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT
    CTGCAACACA GGTATTCTGA ACACACTCAG CTACAGACTC AGCAGAGAGC TGTTCAGGAG
    GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACATTATCA GGCTGCATTC
    AATAATTTAG AAGCAACACA GCTGGCAAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC
    CTAGGTCCTC CAAGCTATGT GCCAGCAACA GCCTTTCTTC AGAACGCTGG CCAGGCCCAC
    TTGATTAGCC AGTGTGAGCA GTTAGAGGGG GAAGTTGGCG CCCTCCTGCA GCAGAGGCGC
    TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTATG CAACTGTAGC TCTGCAATAT
    CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAACTT
    ATCTGTAACA CCACTGTAGA GCGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT
    GCACCCCAGC CGCCCCCAAC AGTGTGTCAG TTCATCACTG CCACTGAGAT GACCCTGCAG
    CGGTACGCAG CAGATATAAA CAGTAGGCTT ATTAGACAGG TGGAACGATT GAAACAAGAA
    GCCGTCACCG TACCTGTTTG TGAAGATCAG CTGAAAGAAA TTGAACGTTG CATCAAAGTT
    TTCCTTCATG AGAATGGAGA AGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATCTCTGCC
    CTTTGTACCC TTACAAGGCG TAACCTGATG ATGGAAGGGG CAGCTTCGAG TGCTGGAGAG
    CAGTTGGTGG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAGCT CTGCAGTATG
    AGTGGAAATG TCACTTGCCT TGTTCAGTTA CTGAAGCAGT GTCATCTGGT GCCACAGGAC
    TTAGACATCC CTAACCCCAT GGAGGCGTCT GAGGCAGTTC ACTTAGCTAA TGGAGTATAC
    ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA
    TGTTTAATGA AAGGCGAATA CACATTAGAA AGTATGCTTC ATGAACTGGA CAGCCTTATT
    GAGCAGACCA CTGATGGCGT TCCTCTGCAG ACTCTAGTGG AATCTCTTCA AGCCTATTTA
    AGAAACGCAG CCATGGGACT TGAAGAAGAA ACACATGCCC ATTACATCGA TGTTGCCAGA
    ATACTTCATG CTCAATATGG TGAGTTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA
    CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCCTTTG ATGGCATGTT TGCCCAAGTT
    GAGACTGCTT TTGGCTTATT AGTTGAAAAG CTAAACAAGA TGGAAATTCC TATCGCTTGG
    AGAAAGATTG ACATTATAAG GGAAGCCAGG AGCACTCAAG TCAACTTTTT TGATGATGAT
    AATCACCGAC AGGTGCTGGA AGAGATTTTC TTTCTAAAAC GACTGCAGAC AATTAAGGAG
    TTCTTCAGAC TCTGCGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT
    CAGAATACTG TAAATGGGCC TGTACCGATT GTCAACGTGA AAACTCTTTT TAGGAACTCT
    TGTTTCAGTG AAGACCAGAT GGCCAAACCT ATAAAGGCCT TCACAGCTGA CTTCGTGCGG
    CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CGCTGTGCAG CTTCATCAGT
    GCCCTAGGAG TGGACATCAT TGCTCAAGTG GAGGCCAAGG ATTTTGGTGC TGAAAGCAAG
    GTTTCTGTTG ATGATCTGTG TAAGAAAGCA GTGGAGCACA ACATCCAGGT CGGGAAGTTC
    TCTCAGCTGG TCATGAACAG GGCGACTGTG CTCGCCAGCT CCTATGACAC TGCCTGGAAG
    AAGCACGACT TGGTGCGCAG GCTGGAGACC AGTATCTCCT CCTGTAAGAC GAGCCTGCAG
    CGCGTTCAGC TGCATATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAACAGA
    CCACAAGCGA TGTCGGTCAC ACCTCCCCCA CGATCTGCCA TCCTAACCAG CATGAAAAAG
    AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA
    GCTCTTGAAG CAAGTATTGA ACAGCGACTC AAGTGGGCAG GTGGTGCCAA CCCTGCACTG
    GCACCAGTAC TACAAGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAA
    GAGAGCCAAA GAGCCAGTCA GGTCACTTTC CTCTGCAGCA ATATTATTCA TTTTGAAAGT
    TTACGAACTA GAACTGCGGA AGCCTTAAAC CTGGATGCTG CATTATTTGA GCTAATCAAA
    CGGTGTCAGC AAATGTGTTC ATTTGCTTCA CAGTTTAACA GTTCAGTCTC TGAGTTAGAG
    CTTCGTTTAT TACAGAGAGT GGACACAAGC CTTGAACACC CTATTGGCAG CTCTGAATGG
    CTTTTATCAG CACACAAACA GTTGACCCAG GATATGTCTA CGCAGAGAGC AATTCAGACG
    GAGAAAGAGC AGCAGATAGA AACGGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA
    AAGACTGTGC TCACAGGTCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA
    ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGCGAAG ATGTTCCCTA TGAGAACAGT
    GTGAGGCAGT TCTTGGGTGA ATATAAATCT TGGCAAGACA ACATTCAGAC TGTGCTGTTT
    ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAGGAAC ATGTTGAAAT GCTCCAGGAA
    ATAACTCCCA CCTTGAAAGA ACTGAAAACA CAAAGTCAGA GTGTCTATAA TAATTTAGTG
    AGTTTTGCAT CACCACTAGT CACCGATGCA ACAAATGAAT GTTCAAGTCC AACGTCATCT
    GCTACGTATC AGCCATCCTT TGCTGCAGCA GTCCGGAGCA ACACTGGCCA GAAGACTCAG
    CCTGATGTCA TGTCACAGAA TGCTAGAAAG CTGATTCAGA AAAATCTAGC TACATCAGCA
    GATACTCCAC CAAGCACTGT TCCAGGAACT GGCAAGAGTG CTGCTTGTAG TCCTAAAAAG
    GCCGTCAGAG ACCCTAAAAC TGGGAAAGCT GTGCAAGAGA GAAATTCATA TGCAGTGAGC
    GTGTGGAAGA GAGTGAAAGC CAAGTTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG
    TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT
    CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe46686
    Clone ID Related Accession (Same CDS sequence) XM_005658617.2
    Accession Version XM_005658617.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product LOW QUALITY PROTEIN: serine/threonine-protein kinase SMG1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003539373.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005658617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAT TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAACCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA CGATGATACC
    AGAATCCACG CTGACATACA AAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TCACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGACGA CAGAGACCGA AGACTGGCTA CTGTAAAGCA GTTGAAAGAG
    TTTATTCAGC AACCAGAAAA TAAGCTGGTA CTAGTTAAAC AATTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT CTGAGCTATG AGGCCGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TGAAACTTCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAATGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACTAAT
    TTTAGGGATA CAGTTGATAT ATTAGTTTGA TGGCATATAG ANGATCATAC TCAGAAGCCT
    TCCCTCACAC AGCAGGTGTC TGGGTGGTTG CAGAGTTTGG AGCCATTCTG GGTAGCTGAT
    CTTGCATTTT CTACCACTCT TCTTGGTCAG TTTCTAGAGG ACATGGAGGC ATATGCTGAG
    GACCTCAGCC ATGTGGCCTC CGGGGAGTCA GTGGATGAAG ACGTTCCTCC TCCGTCAGTG
    TCCTTACCAA AGCTGGCAGC TCTCCTCCGG GTATTCAGTA CTGTGGTGAG AAGCATTGGG
    GAACGTTTCA GCCCAATTCG GGGTCCTCCA ATTACAGAGG CATATGTAAC AGATGTTCTG
    TACAGAGTAA TGAGATGTGT GACGGCTGCA AACCAGGTGT TTTTTTCTGA GGCTGTGTTG
    ACAGCTGCTA ATGAGTGTGT TGGTGTTTTG CTCGGCAGCT TGGATCCTAG CATGACTATA
    CATTGTGACA TGGTCATTAC ATATGGATTA GATCAGCTGG AGAATTGCCA GACTTGTGGT
    ACAGATTATA TCATCTCAGT CTTGAATTTA CTCACATTGA TTGTTGAACA GATAAATACA
    AAATTGCCTT CATCATTTGT AGAAAAACTA TTTATACCAT CATCTAAACT ACTATTTTTG
    CGTTATCATA AAGAAAAAGA GGTTGTTGCT GTAGCCCATG CTGTTTATCA AGCAGTGCTC
    AGCCTGAAGA ATATTCCTGT TTTGGAGACT GCCTATAAAT TAATTTTGGG AGAAATGACT
    TGTGCCCTAA ACAACCTGCT ACATAGTCTA CAACTTCCCG ATGCCTGTTC TGAAATAAAA
    CATGAGGCTT TTAAGAATCA TTTATTCAAC GTAGACAATG CAAAATTTGT AGTTATATTT
    GACCTCAGTG CTCTGACTAC AATTGGGAAT GCTAAAAATT CACTAATTGG GATGTGGGCG
    CTGTCTCCAA CCGTCTTTGC ACTCCTGAGT AAGAATCTGA TGATTGTGCA TGGTGACCTG
    GCCGTTCACT TCCCTGCCAT TCAGTATGCA GTGCTCTACA CGTTGTATTC ACATTGTACT
    AGGCATGACC ACTTTATATC TAGTAGTCTG AGTTCTTCGT CCCCATCTTT GTTTGATGGA
    GCCGTGATTA GTACTGTAAC CACAGCCACC AAAAAACATT TCTCAATTAT ATTAAATCTC
    TTGGGAATAT TACTAAAGAA GGATAATCTT AACCAGGACA CAAGGAAACT GTTAATGACC
    TGGGCTTTGG AAGTGGCTGT TTTAATGAAG AAGTCTGAAA CATACGCACC TTTATTCTCT
    CTGCCATCTT TCCATAAGTT TTCCAAAGGC CTTTTAGCCA ACACTCTTGT TGAAGATGTG
    AATATCTGTC TGCAAGCCTG CAGCAGTCTA CATGCTTTGT CCTCGTCTTT ACCGGATGAT
    CTTTTACAGA GGTGTGTTGA TGTTTGCCGT GTTCAACTAG TCCATAGTGG AACTCGTATT
    CGCCAGGCAT TTGGAAAGCT ATTGAAGTAA ATTCCTTTAG ATGTTGTCTT GAGCAATAAC
    AATCACACAG AAATTCAAGA AATTTCTTTA GCATTAAGAA GTCACATGAG TAAAGCACCA
    AGTAATACAT TCCACCCACA AGATTTCTCT GATGTTATTA GTTTTATTTT GTATGGGAAC
    TCTCATAGAA CGGAGAAGGA CAATTGGTTA GAAAGATTGT TCTACAGCTG CCAGAGACTG
    GATAAACGTG ACCAATCAAC AATTCCCCGC AATCTTCTAA AGACAGATGC TGTCCTTTGG
    CAGTGGGCTA TTTGGGAAGC AGCACAGTTC ACTGTCCTTT CTAAGTTGAG AACTCCACTG
    GGCAGAGCTC AGGATACCTT CCAGACAATT GAAGGTATCA TCAGAAGTCT TGCAGCTCAC
    ACCTTAAACC CTGATCAGGA TGTTAGTCAG TGGACAACTG CAGACAATGA TGAAGGCCAC
    AGTAGCAACC AGCTTAGACT TGTGCTTCTT CTGCAGTATC TGGAAAACCT GGAGAAGTTA
    ATGTATAATG CATATGAGGG ATGTGCCAAT GCGTTGACAT CACCTCCCAA GGTCATTAGG
    ACTTTTTTCT ATACCAACCG CCAGACTTGT CAAGACTGGC TGACCCGAAT CCGACTCTCC
    ATCATGAGGG TTGGATTGTT AGCCGGCCAG CCTGCTGTGA CAGTGAGACA TGGCTTTGAT
    TTGCTTACGG AAATGAAGAC AAATCCATCT CAGGGGAATG AATTGGAAGT AACTATCATG
    ATGGTGGTAG AAGCACTGTG TGAACTTCAT TGTCCAGAAG CTATACAAGG AATTGCTGTC
    TGGTCATCTT CCATCGTTGG AAAAAATCTT CTCTGGATTA ATTCAGTGGC CCAACAGGCT
    GAAGGGAGGT TTGAAAAGGC CTCCGTGGAG TACCAGGAGC ACCTGTGTGC CATGACAGGT
    GTGGATTGCT GCATCTCCAG CTTTGACAAG TCTGTTCTCA CATTGGCTAA TACTGGGCGT
    AACAGTGCCA GCCCCAAACA TTCTTTGAAC GGTGAATCCA GAAAAACTGT GCTGTCCAAA
    CCAACTGACT CTTCTCCTGA GGTTATAAAT TATTTGGGAA ATAAAGCATG TGAGTGCTAC
    ATTGCAATTG CCGATTGGAC TGCTGTACAG GAATGGCAGA ATGCTATCCA TGACTTGAAA
    AAGAGAACCA GCAGTANTAC TTCTCTCAAT CTAAAAGCTG ATTTCAACTA CATAAAATCA
    CTAAGCAGTT TTGAATCTGG AGAATTTGTT GAATGTACTG AGCAATTGGA ATTGTTACCA
    GGAGAAAATA TCAATCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTA
    CTTCCTAATA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATCGA AGTTCAATTG
    CTGAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCATA TAGAACAGGA TCAGAAGTGG
    CAGTCTATAA CTGAAAACGT GGTGAAGTAC TTGAAGCAAA CCTCCCGCAT AGCTATCGGG
    CCACTGAGAC TTTCTACTTT AACAGTTTCA CAGTCTCTGC CAGTTCTAAG TACCTTGCAG
    CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTTCAAC AGAGGACTGT
    CTTATCCCAC TCTTCAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG
    ATGCAGGCAT TAAGATACAC CATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA
    ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA
    AAACGGGGGA ATGTGTCACT TGCAACAAGA CTGCTGGCAC AGTGCAGTGA GGTTCAGCTG
    GCAAAGACCA CGACTGCGCA GGATTTAGTC CAGCATTTTA AAAAGCTATC AACTCAAGGT
    CAAGTGGATG AAAAGTGGGG GCCTGAACTT GACATTGAAA AAACCAAATT GCTATATACA
    GCAGGCCAGT CAACACACGC AATGGAGATG CTGAGTTCTT GCGCCATTTC GTTCTGCAAG
    TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCCATTCTTA CACTGGCCAA ATGGATCCAG
    GCAGAATGGA AAGAAATATC AGGGCAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAA
    AACTTCACAG GTCTTTCTAC TTTATCTAAA AACATACTCA CTCTAATTGA ACTCCCATCT
    GTTAATACAG TGGAAGAAGA GTATCCTCGA ATCGAGAGCG AATCTACAGT ACATATCGGA
    GTTGGAGAAC CTGACTTCAT TNNNNNNNNN NNNNNNNNNN NNTCTTCAGT ACAGGCACCT
    GAAGTAGCCA AATCTTGGGC AGCATTGGCT AGTTGGGCTT ATAGATGGGG CAGAAAGGTC
    GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CCAGAGAAAA ATCTGAAGTT
    CAGAATCTGC TCCCAGACAC TATCACTGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT
    GGGCAGGCAG TGTGTCGGCC AGCAGGAATT CAGGATGAAG ATATAACGCT TCAGATAACA
    GAAAGTGAAG ATAATGAGGA AGATGATATG GTTGATGTTA TCTGGCGTCA GTTAATATCG
    AGCTGCCCCT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGCGTTAT TAAAGTGTGG
    AGGAAAGTTG TAGATAGAAT CTTCAGCCTG TACAAACTGT CTTGCAGCGC CTATTTTACT
    TTCCTTAAGC TCAATGCTGG TCAGATTCCT TTAGATGAAG ATGACCCCAG GCTCCATTTA
    AGTCACAGAC CGGAGCAGAG CACGGATGAC GTGATTGTGA TGGCCACATT GAGGCTGCTC
    AGACTGCTCG TGAAACATGC CGGCGAGCTC AGGCAGTATC TGGAACATGG CTTGGAAACA
    ACACCCACTG CTCCCTGGAG AGGAATTATT CCACAACTTT TCTCACGCTT AAACCACCCT
    GAAGTGTATG TGCGCCAAAG TATTTGTAAC CTTCTCTGCC GTGTTGCTCA GGATTCCCCC
    CATCTCATAT TGTACCCTGC GATAGTGGGC ACCATATCGC TTAGCAGTGA GTCACAGGCT
    TCAGGAAATA AGTTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA
    TTGCTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CCCAAGATAG CAATAAGGAT
    GAACCGAAGA GTGGATTGAA TGAAGACCAA GCTATGATGC AGGATTGCTA CAGCAAGATT
    GTAGATAAAC TGTCCTCTGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTCGTGGCC
    GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG
    CACATGTATG TCCTGAGACG AATTCAGCAG CTGGAAGACG AAGTGAAGAG AGTCCAGAAT
    AACAACACCT TACGCAAAGA AGAGAAAATT GCAATCATGC GAGAGAAGCA CACAGCTTTG
    ATGAAGCCCA TTGTGTTTGC TTTGGAGCAC GTGAGGAGTA TCACGGCGGC CCCTGCAGAA
    ACGCCTCATG AAAAGTGGTT TCAAGATAAC TATGGTGATG CAATTGAAAA TGCCCTAGAA
    AAACTGAAGA CACCATCAAA CCCTGCAAAG CCTGGAAGCA GTTGGATTCC ATTTAAAGAG
    ATAATGCTAA GTTTGCAACA GAGAGCACAG AAACGTGCAA GCTACATCTT GCGTCTTGAA
    GAAATCAGCC CGTGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC TGGAGAAGTC
    TCAGCCAGAG ACACTGTCAC AATCCACAGT GTGGGCGGAA CCATCACAAT CTTACCCACC
    AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCGGATG GGAAGAGCTA TCCTTATCTT
    TTCAAAGGAT TGGAGGACTT ACATCTGGAT GAGAGAATAA TGCAGTTTTT ATCTATTGTA
    AATACTATGT TTGCTACAAT CAATCGCCAG GAGACACCCC GCTTCCATGC GCGGCACTAT
    TCTGTAACAC CACTGGGAAC TCGATCAGGA CTGATCCAGT GGGTGGATGG AGCCACACCC
    TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCTG CCTTACAAGC ACAAAAGGCC
    CAAGATTCCT ACCAGACTCC TCAGAATCCG GGAATTGTAC CCCGTCCTAG TGAACTCTAT
    TATAGTAAAA TCGGTCCTGC TTTGAAAGCA GTGGGACTTA GTCTGGATGT GTCCCGACGG
    GATTGGCCTC TTCATGTGAT GAAGGCAGTG CTAGAAGAGT TGATGGAAGC CACGCCTCCA
    AATCTCCTTG CTAAAGAGCT GTGGTCATCT TGTACCACCC CTGATGAATG GTGGAGAGTC
    ACACAGTCTT ACGCCAGATC GACCGCGGTC ATGTCTATGG TCGGGTACAT AATTGGCCTT
    GGAGACAGAC ATCTGGATAA CGTCCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA
    GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTTCCTTTT
    CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGA
    CTTTCATGTG AGCAGGTTTT GCACATCATG CGGCGTGGCA GAGAGACTCT GCTGACACTA
    CTGGAGGCCT TTGTGTACGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT
    GCTGGTGCCG TCTACGGTGG GGGAGGCCAG CAGGCCGAGA GCAAGCAGAG CAAGAGAGAG
    ATGGAAAGAG AAATCACCCG CAGCCTGTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC
    TGGTTTAAGA ATAGGGACGA GATGCTGGTT GTACTGCCCA AGCTGGACAG CAGCTTAGAT
    GAATACCTGA GCTTGCAAGA GCAACTGACA GATGTGGAAA AACTGCAGGG CAAACTTCTG
    GAAGAAATAG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA
    CACAGGTATT CTGAACACAC TCAGCTACAG ACTCAGCAGA GAGCTGTTCA GGAGGCAATC
    CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACATT ATCAGGCTGC ATTCAATAAT
    TTAGAAGCAA CACAGCTGGC AAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTAGGT
    CCTCCAAGCT ATGTGCCAGC AACAGCCTTT CTTCAGAACG CTGGCCAGGC CCACTTGATT
    AGCCAGTGTG AGCAGTTAGA GGGGGAAGTT GGCGCCCTCC TGCAGCAGAG GCGCTCGGTG
    CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TATGCAACTG TAGCTCTGCA ATATCCAAAG
    GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA ACTTATCTGT
    AACACCACTG TAGAGCGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCACCC
    CAGCCGCCCC CAACAGTGTG TCAGTTCATC ACTGCCACTG AGATGACCCT GCAGCGGTAC
    GCAGCAGATA TAAACAGTAG GCTTATTAGA CAGGTGGAAC GATTGAAACA AGAAGCCGTC
    ACCGTACCTG TTTGTGAAGA TCAGCTGAAA GAAATTGAAC GTTGCATCAA AGTTTTCCTT
    CATGAGAATG GAGAAGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATCTC TGCCCTTTGT
    ACCCTTACAA GGCGTAACCT GATGATGGAA GGGGCAGCTT CGAGTGCTGG AGAGCAGTTG
    GTGGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AGCTCTGCAG TATGAGTGGA
    AATGTCACTT GCCTTGTTCA GTTACTGAAG CAGTGTCATC TGGTGCCACA GGACTTAGAC
    ATCCCTAACC CCATGGAGGC GTCTGAGGCA GTTCACTTAG CTAATGGAGT ATACACCTCA
    CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA
    ATGAAAGGCG AATACACATT AGAAAGTATG CTTCATGAAC TGGACAGCCT TATTGAGCAG
    ACCACTGATG GCGTTCCTCT GCAGACTCTA GTGGAATCTC TTCAAGCCTA TTTAAGAAAC
    GCAGCCATGG GACTTGAAGA AGAAACACAT GCCCATTACA TCGATGTTGC CAGAATACTT
    CATGCTCAAT ATGGTGAGTT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA
    ATGTCAGCTG GCCAGATGCT TTTGGTAGCC TTTGATGGCA TGTTTGCCCA AGTTGAGACT
    GCTTTTGGCT TATTAGTTGA AAAGCTAAAC AAGATGGAAA TTCCTATCGC TTGGAGAAAG
    ATTGACATTA TAAGGGAAGC CAGGAGCACT CAAGTCAACT TTTTTGATGA TGATAATCAC
    CGACAGGTGC TGGAAGAGAT TTTCTTTCTA AAACGACTGC AGACAATTAA GGAGTTCTTC
    AGACTCTGCG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT
    ACTGTAAATG GGCCTGTACC GATTGTCAAC GTGAAAACTC TTTTTAGGAA CTCTTGTTTC
    AGTGAAGACC AGATGGCCAA ACCTATAAAG GCCTTCACAG CTGACTTCGT GCGGCAGCTC
    CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACGCTGT GCAGCTTCAT CAGTGCCCTA
    GGAGTGGACA TCATTGCTCA AGTGGAGGCC AAGGATTTTG GTGCTGAAAG CAAGGTTTCT
    GTTGATGATC TGTGTAAGAA AGCAGTGGAG CACAACATCC AGGTCGGGAA GTTCTCTCAG
    CTGGTCATGA ACAGGGCGAC TGTGCTCGCC AGCTCCTATG ACACTGCCTG GAAGAAGCAC
    GACTTGGTGC GCAGGCTGGA GACCAGTATC TCCTCCTGTA AGACGAGCCT GCAGCGCGTT
    CAGCTGCATA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA CAGACCACAA
    GCGATGTCGG TCACACCTCC CCCACGATCT GCCATCCTAA CCAGCATGAA AAAGAAACTC
    CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCTCTT
    GAAGCAAGTA TTGAACAGCG ACTCAAGTGG GCAGGTGGTG CCAACCCTGC ACTGGCACCA
    GTACTACAAG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAAGAGAGC
    CAAAGAGCCA GTCAGGTCAC TTTCCTCTGC AGCAATATTA TTCATTTTGA AAGTTTACGA
    ACTAGAACTG CGGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAGCTAAT CAAACGGTGT
    CAGCAAATGT GTTCATTTGC TTCACAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT
    TTATTACAGA GAGTGGACAC AAGCCTTGAA CACCCTATTG GCAGCTCTGA ATGGCTTTTA
    TCAGCACACA AACAGTTGAC CCAGGATATG TCTACGCAGA GAGCAATTCA GACGGAGAAA
    GAGCAGCAGA TAGAAACGGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT
    GTGCTCACAG GTCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC
    AAGGATGAAG AAGCTGCTCT GGCAGATGGC GAAGATGTTC CCTATGAGAA CAGTGTGAGG
    CAGTTCTTGG GTGAATATAA ATCTTGGCAA GACAACATTC AGACTGTGCT GTTTACATTA
    GTCCAAGCCA TGGGTCAGGT TCGAAGTCAG GAACATGTTG AAATGCTCCA GGAAATAACT
    CCCACCTTGA AAGAACTGAA AACACAAAGT CAGAGTGTCT ATAATAATTT AGTGAGTTTT
    GCATCACCAC TAGTCACCGA TGCAACAAAT GAATGTTCAA GTCCAACGTC ATCTGCTACG
    TATCAGCCAT CCTTTGCTGC AGCAGTCCGG AGCAACACTG GCCAGAAGAC TCAGCCTGAT
    GTCATGTCAC AGAATGCTAG AAAGCTGATT CAGAAAAATC TAGCTACATC AGCAGATACT
    CCACCAAGCA CTGTTCCAGG AACTGGCAAG AGTGCTGCTT GTAGTCCTAA AAAGGCCGTC
    AGAGACCCTA AAACTGGGAA AGCTGTGCAA GAGAGAAATT CATATGCAGT GAGCGTGTGG
    AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT
    GCTGAACAGG TAACCAATCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005658674.2
    CDS27..10910
    Translation

    Target ORF information:

    RefSeq Version XM_005658617.2
    Organism Sus scrofa(Pig)
    Definition Sus scrofa SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005658617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAT TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAACCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA CGATGATACC
    AGAATCCACG CTGACATACA AAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TCACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGACGA CAGAGACCGA AGACTGGCTA CTGTAAAGCA GTTGAAAGAG
    TTTATTCAGC AACCAGAAAA TAAGCTGGTA CTAGTTAAAC AATTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGCTGTC TTGGCCTTCT TTGTGCTTCT CTGAGCTATG AGGCCGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TGAAACTTCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAATGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACTAAT
    TTTAGGGATA CAGTTGATAT ATTAGTTTGA TGGCATATAG ANGATCATAC TCAGAAGCCT
    TCCCTCACAC AGCAGGTGTC TGGGTGGTTG CAGAGTTTGG AGCCATTCTG GGTAGCTGAT
    CTTGCATTTT CTACCACTCT TCTTGGTCAG TTTCTAGAGG ACATGGAGGC ATATGCTGAG
    GACCTCAGCC ATGTGGCCTC CGGGGAGTCA GTGGATGAAG ACGTTCCTCC TCCGTCAGTG
    TCCTTACCAA AGCTGGCAGC TCTCCTCCGG GTATTCAGTA CTGTGGTGAG AAGCATTGGG
    GAACGTTTCA GCCCAATTCG GGGTCCTCCA ATTACAGAGG CATATGTAAC AGATGTTCTG
    TACAGAGTAA TGAGATGTGT GACGGCTGCA AACCAGGTGT TTTTTTCTGA GGCTGTGTTG
    ACAGCTGCTA ATGAGTGTGT TGGTGTTTTG CTCGGCAGCT TGGATCCTAG CATGACTATA
    CATTGTGACA TGGTCATTAC ATATGGATTA GATCAGCTGG AGAATTGCCA GACTTGTGGT
    ACAGATTATA TCATCTCAGT CTTGAATTTA CTCACATTGA TTGTTGAACA GATAAATACA
    AAATTGCCTT CATCATTTGT AGAAAAACTA TTTATACCAT CATCTAAACT ACTATTTTTG
    CGTTATCATA AAGAAAAAGA GGTTGTTGCT GTAGCCCATG CTGTTTATCA AGCAGTGCTC
    AGCCTGAAGA ATATTCCTGT TTTGGAGACT GCCTATAAAT TAATTTTGGG AGAAATGACT
    TGTGCCCTAA ACAACCTGCT ACATAGTCTA CAACTTCCCG ATGCCTGTTC TGAAATAAAA
    CATGAGGCTT TTAAGAATCA TTTATTCAAC GTAGACAATG CAAAATTTGT AGTTATATTT
    GACCTCAGTG CTCTGACTAC AATTGGGAAT GCTAAAAATT CACTAATTGG GATGTGGGCG
    CTGTCTCCAA CCGTCTTTGC ACTCCTGAGT AAGAATCTGA TGATTGTGCA TGGTGACCTG
    GCCGTTCACT TCCCTGCCAT TCAGTATGCA GTGCTCTACA CGTTGTATTC ACATTGTACT
    AGGCATGACC ACTTTATATC TAGTAGTCTG AGTTCTTCGT CCCCATCTTT GTTTGATGGA
    GCCGTGATTA GTACTGTAAC CACAGCCACC AAAAAACATT TCTCAATTAT ATTAAATCTC
    TTGGGAATAT TACTAAAGAA GGATAATCTT AACCAGGACA CAAGGAAACT GTTAATGACC
    TGGGCTTTGG AAGTGGCTGT TTTAATGAAG AAGTCTGAAA CATACGCACC TTTATTCTCT
    CTGCCATCTT TCCATAAGTT TTCCAAAGGC CTTTTAGCCA ACACTCTTGT TGAAGATGTG
    AATATCTGTC TGCAAGCCTG CAGCAGTCTA CATGCTTTGT CCTCGTCTTT ACCGGATGAT
    CTTTTACAGA GGTGTGTTGA TGTTTGCCGT GTTCAACTAG TCCATAGTGG AACTCGTATT
    CGCCAGGCAT TTGGAAAGCT ATTGAAGTAA ATTCCTTTAG ATGTTGTCTT GAGCAATAAC
    AATCACACAG AAATTCAAGA AATTTCTTTA GCATTAAGAA GTCACATGAG TAAAGCACCA
    AGTAATACAT TCCACCCACA AGATTTCTCT GATGTTATTA GTTTTATTTT GTATGGGAAC
    TCTCATAGAA CGGAGAAGGA CAATTGGTTA GAAAGATTGT TCTACAGCTG CCAGAGACTG
    GATAAACGTG ACCAATCAAC AATTCCCCGC AATCTTCTAA AGACAGATGC TGTCCTTTGG
    CAGTGGGCTA TTTGGGAAGC AGCACAGTTC ACTGTCCTTT CTAAGTTGAG AACTCCACTG
    GGCAGAGCTC AGGATACCTT CCAGACAATT GAAGGTATCA TCAGAAGTCT TGCAGCTCAC
    ACCTTAAACC CTGATCAGGA TGTTAGTCAG TGGACAACTG CAGACAATGA TGAAGGCCAC
    AGTAGCAACC AGCTTAGACT TGTGCTTCTT CTGCAGTATC TGGAAAACCT GGAGAAGTTA
    ATGTATAATG CATATGAGGG ATGTGCCAAT GCGTTGACAT CACCTCCCAA GGTCATTAGG
    ACTTTTTTCT ATACCAACCG CCAGACTTGT CAAGACTGGC TGACCCGAAT CCGACTCTCC
    ATCATGAGGG TTGGATTGTT AGCCGGCCAG CCTGCTGTGA CAGTGAGACA TGGCTTTGAT
    TTGCTTACGG AAATGAAGAC AAATCCATCT CAGGGGAATG AATTGGAAGT AACTATCATG
    ATGGTGGTAG AAGCACTGTG TGAACTTCAT TGTCCAGAAG CTATACAAGG AATTGCTGTC
    TGGTCATCTT CCATCGTTGG AAAAAATCTT CTCTGGATTA ATTCAGTGGC CCAACAGGCT
    GAAGGGAGGT TTGAAAAGGC CTCCGTGGAG TACCAGGAGC ACCTGTGTGC CATGACAGGT
    GTGGATTGCT GCATCTCCAG CTTTGACAAG TCTGTTCTCA CATTGGCTAA TACTGGGCGT
    AACAGTGCCA GCCCCAAACA TTCTTTGAAC GGTGAATCCA GAAAAACTGT GCTGTCCAAA
    CCAACTGACT CTTCTCCTGA GGTTATAAAT TATTTGGGAA ATAAAGCATG TGAGTGCTAC
    ATTGCAATTG CCGATTGGAC TGCTGTACAG GAATGGCAGA ATGCTATCCA TGACTTGAAA
    AAGAGAACCA GCAGTANTAC TTCTCTCAAT CTAAAAGCTG ATTTCAACTA CATAAAATCA
    CTAAGCAGTT TTGAATCTGG AGAATTTGTT GAATGTACTG AGCAATTGGA ATTGTTACCA
    GGAGAAAATA TCAATCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTA
    CTTCCTAATA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATCGA AGTTCAATTG
    CTGAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCATA TAGAACAGGA TCAGAAGTGG
    CAGTCTATAA CTGAAAACGT GGTGAAGTAC TTGAAGCAAA CCTCCCGCAT AGCTATCGGG
    CCACTGAGAC TTTCTACTTT AACAGTTTCA CAGTCTCTGC CAGTTCTAAG TACCTTGCAG
    CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTTCAAC AGAGGACTGT
    CTTATCCCAC TCTTCAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG
    ATGCAGGCAT TAAGATACAC CATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA
    ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA
    AAACGGGGGA ATGTGTCACT TGCAACAAGA CTGCTGGCAC AGTGCAGTGA GGTTCAGCTG
    GCAAAGACCA CGACTGCGCA GGATTTAGTC CAGCATTTTA AAAAGCTATC AACTCAAGGT
    CAAGTGGATG AAAAGTGGGG GCCTGAACTT GACATTGAAA AAACCAAATT GCTATATACA
    GCAGGCCAGT CAACACACGC AATGGAGATG CTGAGTTCTT GCGCCATTTC GTTCTGCAAG
    TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCCATTCTTA CACTGGCCAA ATGGATCCAG
    GCAGAATGGA AAGAAATATC AGGGCAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAA
    AACTTCACAG GTCTTTCTAC TTTATCTAAA AACATACTCA CTCTAATTGA ACTCCCATCT
    GTTAATACAG TGGAAGAAGA GTATCCTCGA ATCGAGAGCG AATCTACAGT ACATATCGGA
    GTTGGAGAAC CTGACTTCAT TNNNNNNNNN NNNNNNNNNN NNTCTTCAGT ACAGGCACCT
    GAAGTAGCCA AATCTTGGGC AGCATTGGCT AGTTGGGCTT ATAGATGGGG CAGAAAGGTC
    GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CCAGAGAAAA ATCTGAAGTT
    CAGAATCTGC TCCCAGACAC TATCACTGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT
    GGGCAGGCAG TGTGTCGGCC AGCAGGAATT CAGGATGAAG ATATAACGCT TCAGATAACA
    GAAAGTGAAG ATAATGAGGA AGATGATATG GTTGATGTTA TCTGGCGTCA GTTAATATCG
    AGCTGCCCCT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGCGTTAT TAAAGTGTGG
    AGGAAAGTTG TAGATAGAAT CTTCAGCCTG TACAAACTGT CTTGCAGCGC CTATTTTACT
    TTCCTTAAGC TCAATGCTGG TCAGATTCCT TTAGATGAAG ATGACCCCAG GCTCCATTTA
    AGTCACAGAC CGGAGCAGAG CACGGATGAC GTGATTGTGA TGGCCACATT GAGGCTGCTC
    AGACTGCTCG TGAAACATGC CGGCGAGCTC AGGCAGTATC TGGAACATGG CTTGGAAACA
    ACACCCACTG CTCCCTGGAG AGGAATTATT CCACAACTTT TCTCACGCTT AAACCACCCT
    GAAGTGTATG TGCGCCAAAG TATTTGTAAC CTTCTCTGCC GTGTTGCTCA GGATTCCCCC
    CATCTCATAT TGTACCCTGC GATAGTGGGC ACCATATCGC TTAGCAGTGA GTCACAGGCT
    TCAGGAAATA AGTTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA
    TTGCTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CCCAAGATAG CAATAAGGAT
    GAACCGAAGA GTGGATTGAA TGAAGACCAA GCTATGATGC AGGATTGCTA CAGCAAGATT
    GTAGATAAAC TGTCCTCTGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTCGTGGCC
    GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG
    CACATGTATG TCCTGAGACG AATTCAGCAG CTGGAAGACG AAGTGAAGAG AGTCCAGAAT
    AACAACACCT TACGCAAAGA AGAGAAAATT GCAATCATGC GAGAGAAGCA CACAGCTTTG
    ATGAAGCCCA TTGTGTTTGC TTTGGAGCAC GTGAGGAGTA TCACGGCGGC CCCTGCAGAA
    ACGCCTCATG AAAAGTGGTT TCAAGATAAC TATGGTGATG CAATTGAAAA TGCCCTAGAA
    AAACTGAAGA CACCATCAAA CCCTGCAAAG CCTGGAAGCA GTTGGATTCC ATTTAAAGAG
    ATAATGCTAA GTTTGCAACA GAGAGCACAG AAACGTGCAA GCTACATCTT GCGTCTTGAA
    GAAATCAGCC CGTGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC TGGAGAAGTC
    TCAGCCAGAG ACACTGTCAC AATCCACAGT GTGGGCGGAA CCATCACAAT CTTACCCACC
    AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCGGATG GGAAGAGCTA TCCTTATCTT
    TTCAAAGGAT TGGAGGACTT ACATCTGGAT GAGAGAATAA TGCAGTTTTT ATCTATTGTA
    AATACTATGT TTGCTACAAT CAATCGCCAG GAGACACCCC GCTTCCATGC GCGGCACTAT
    TCTGTAACAC CACTGGGAAC TCGATCAGGA CTGATCCAGT GGGTGGATGG AGCCACACCC
    TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCTG CCTTACAAGC ACAAAAGGCC
    CAAGATTCCT ACCAGACTCC TCAGAATCCG GGAATTGTAC CCCGTCCTAG TGAACTCTAT
    TATAGTAAAA TCGGTCCTGC TTTGAAAGCA GTGGGACTTA GTCTGGATGT GTCCCGACGG
    GATTGGCCTC TTCATGTGAT GAAGGCAGTG CTAGAAGAGT TGATGGAAGC CACGCCTCCA
    AATCTCCTTG CTAAAGAGCT GTGGTCATCT TGTACCACCC CTGATGAATG GTGGAGAGTC
    ACACAGTCTT ACGCCAGATC GACCGCGGTC ATGTCTATGG TCGGGTACAT AATTGGCCTT
    GGAGACAGAC ATCTGGATAA CGTCCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA
    GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTTCCTTTT
    CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGA
    CTTTCATGTG AGCAGGTTTT GCACATCATG CGGCGTGGCA GAGAGACTCT GCTGACACTA
    CTGGAGGCCT TTGTGTACGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT
    GCTGGTGCCG TCTACGGTGG GGGAGGCCAG CAGGCCGAGA GCAAGCAGAG CAAGAGAGAG
    ATGGAAAGAG AAATCACCCG CAGCCTGTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC
    TGGTTTAAGA ATAGGGACGA GATGCTGGTT GTACTGCCCA AGCTGGACAG CAGCTTAGAT
    GAATACCTGA GCTTGCAAGA GCAACTGACA GATGTGGAAA AACTGCAGGG CAAACTTCTG
    GAAGAAATAG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA
    CACAGGTATT CTGAACACAC TCAGCTACAG ACTCAGCAGA GAGCTGTTCA GGAGGCAATC
    CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACATT ATCAGGCTGC ATTCAATAAT
    TTAGAAGCAA CACAGCTGGC AAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTAGGT
    CCTCCAAGCT ATGTGCCAGC AACAGCCTTT CTTCAGAACG CTGGCCAGGC CCACTTGATT
    AGCCAGTGTG AGCAGTTAGA GGGGGAAGTT GGCGCCCTCC TGCAGCAGAG GCGCTCGGTG
    CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TATGCAACTG TAGCTCTGCA ATATCCAAAG
    GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA ACTTATCTGT
    AACACCACTG TAGAGCGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCACCC
    CAGCCGCCCC CAACAGTGTG TCAGTTCATC ACTGCCACTG AGATGACCCT GCAGCGGTAC
    GCAGCAGATA TAAACAGTAG GCTTATTAGA CAGGTGGAAC GATTGAAACA AGAAGCCGTC
    ACCGTACCTG TTTGTGAAGA TCAGCTGAAA GAAATTGAAC GTTGCATCAA AGTTTTCCTT
    CATGAGAATG GAGAAGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATCTC TGCCCTTTGT
    ACCCTTACAA GGCGTAACCT GATGATGGAA GGGGCAGCTT CGAGTGCTGG AGAGCAGTTG
    GTGGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AGCTCTGCAG TATGAGTGGA
    AATGTCACTT GCCTTGTTCA GTTACTGAAG CAGTGTCATC TGGTGCCACA GGACTTAGAC
    ATCCCTAACC CCATGGAGGC GTCTGAGGCA GTTCACTTAG CTAATGGAGT ATACACCTCA
    CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA
    ATGAAAGGCG AATACACATT AGAAAGTATG CTTCATGAAC TGGACAGCCT TATTGAGCAG
    ACCACTGATG GCGTTCCTCT GCAGACTCTA GTGGAATCTC TTCAAGCCTA TTTAAGAAAC
    GCAGCCATGG GACTTGAAGA AGAAACACAT GCCCATTACA TCGATGTTGC CAGAATACTT
    CATGCTCAAT ATGGTGAGTT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA
    ATGTCAGCTG GCCAGATGCT TTTGGTAGCC TTTGATGGCA TGTTTGCCCA AGTTGAGACT
    GCTTTTGGCT TATTAGTTGA AAAGCTAAAC AAGATGGAAA TTCCTATCGC TTGGAGAAAG
    ATTGACATTA TAAGGGAAGC CAGGAGCACT CAAGTCAACT TTTTTGATGA TGATAATCAC
    CGACAGGTGC TGGAAGAGAT TTTCTTTCTA AAACGACTGC AGACAATTAA GGAGTTCTTC
    AGACTCTGCG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT
    ACTGTAAATG GGCCTGTACC GATTGTCAAC GTGAAAACTC TTTTTAGGAA CTCTTGTTTC
    AGTGAAGACC AGATGGCCAA ACCTATAAAG GCCTTCACAG CTGACTTCGT GCGGCAGCTC
    CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACGCTGT GCAGCTTCAT CAGTGCCCTA
    GGAGTGGACA TCATTGCTCA AGTGGAGGCC AAGGATTTTG GTGCTGAAAG CAAGGTTTCT
    GTTGATGATC TGTGTAAGAA AGCAGTGGAG CACAACATCC AGGTCGGGAA GTTCTCTCAG
    CTGGTCATGA ACAGGGCGAC TGTGCTCGCC AGCTCCTATG ACACTGCCTG GAAGAAGCAC
    GACTTGGTGC GCAGGCTGGA GACCAGTATC TCCTCCTGTA AGACGAGCCT GCAGCGCGTT
    CAGCTGCATA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA CAGACCACAA
    GCGATGTCGG TCACACCTCC CCCACGATCT GCCATCCTAA CCAGCATGAA AAAGAAACTC
    CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCTCTT
    GAAGCAAGTA TTGAACAGCG ACTCAAGTGG GCAGGTGGTG CCAACCCTGC ACTGGCACCA
    GTACTACAAG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAAGAGAGC
    CAAAGAGCCA GTCAGGTCAC TTTCCTCTGC AGCAATATTA TTCATTTTGA AAGTTTACGA
    ACTAGAACTG CGGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAGCTAAT CAAACGGTGT
    CAGCAAATGT GTTCATTTGC TTCACAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT
    TTATTACAGA GAGTGGACAC AAGCCTTGAA CACCCTATTG GCAGCTCTGA ATGGCTTTTA
    TCAGCACACA AACAGTTGAC CCAGGATATG TCTACGCAGA GAGCAATTCA GACGGAGAAA
    GAGCAGCAGA TAGAAACGGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT
    GTGCTCACAG GTCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC
    AAGGATGAAG AAGCTGCTCT GGCAGATGGC GAAGATGTTC CCTATGAGAA CAGTGTGAGG
    CAGTTCTTGG GTGAATATAA ATCTTGGCAA GACAACATTC AGACTGTGCT GTTTACATTA
    GTCCAAGCCA TGGGTCAGGT TCGAAGTCAG GAACATGTTG AAATGCTCCA GGAAATAACT
    CCCACCTTGA AAGAACTGAA AACACAAAGT CAGAGTGTCT ATAATAATTT AGTGAGTTTT
    GCATCACCAC TAGTCACCGA TGCAACAAAT GAATGTTCAA GTCCAACGTC ATCTGCTACG
    TATCAGCCAT CCTTTGCTGC AGCAGTCCGG AGCAACACTG GCCAGAAGAC TCAGCCTGAT
    GTCATGTCAC AGAATGCTAG AAAGCTGATT CAGAAAAATC TAGCTACATC AGCAGATACT
    CCACCAAGCA CTGTTCCAGG AACTGGCAAG AGTGCTGCTT GTAGTCCTAA AAAGGCCGTC
    AGAGACCCTA AAACTGGGAA AGCTGTGCAA GAGAGAAATT CATATGCAGT GAGCGTGTGG
    AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT
    GCTGAACAGG TAACCAATCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.