UBE2O cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2O
Entrez Gene ID	100519047
Full Name	ubiquitin conjugating enzyme E2 O
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021066586.1	XP_020922245.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
XM_005656913.2	XP_005656970.1	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X1
XM_013989884.1	XP_013845338.1	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X2
XM_021066587.1	XP_020922246.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
XM_005656913.3	XP_005656970.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
XM_021066588.1	XP_020922247.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X4

Pathways from BioSystems
Gene Ontology

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe233510	XM_021066586.1 Latest version!	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe233511	XM_021066587.1 Latest version!	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe233512	XM_021066588.1 Latest version!	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe27760	XM_005656913.3 Latest version!	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSe40881	XM_013989884.1 Latest version!	Sus scrofa ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSe27760	XM_005656913.2	Sus scrofa ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2O ( XM_021066586.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020922245.1 Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe233510
Clone ID Related Accession (Same CDS sequence)	XM_021066586.1
Accession Version	XM_021066586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010454.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGCC AGCGCCCGCA GCCCCGGCTC CCGCCCAGGC CCCGGACGCG 
GCTCCAGACG CGGCCCCGGC CCTGGACGCA GTCCCCGCCC CGGCCCCGGC GCCAGCGCCC 
GCCCCGGACT CGGCCTCGGG GCCGTCTTCG GACTCCGGCC CCGAAGCCGG CTCGCAGCGG 
CTGCTGTTCT CTCACGACCT GGTGTCGGGC CGTTACCGCG GCTCGGTGCA CTTCGGGCTG 
GTGCGCCTCA TCCACGGCGA GGACTCGGAC TCAGAGGGCG AGGAGGAGGG CCGCGGGAGC 
TCGGGCTGCT CCGAGGCAGG GGGCGCGGGC CACGAGGAGG GCCGGGCCAG CCCGCTGCGC 
CGCGGCTACG TGCGCGTCCA GTGGTACCCG GAGGGCGTCA AGCAGCACGT GAAGGAGACC 
AAGCTGAAGC TGGAGGACCG CTCCGTGGTG CCCCGCGACG TGGTCCGGCA CATGAGATCC 
ACTGACAGCC AGTGCGGCAC TGTGATGGAC GTCAGCATCG ACTGCGCCGT CAAGCTCATC 
GGCACCAACT GCATCATCTA CCCCGTCAAC AGCCGGGACC TCCAGCACAT CTGGCCCTTC 
ATGTATGGGG ACTACATCGC CTACGACTGC TGGCTGGGGA AGGTCTACGA CCTGAAGAAC 
CAGATCATCC TCAAGCTGTC CAACGGCGCC AGGTGCTCCA TGAACACAGA AGACGGCGCT 
AAGCTCTACG ACGTCTGCCC GCATGTCAGC GACTCTGGCC TCTTCTTCGA TGACTCCTAC 
GGCTTCTACC CAGGCCAGGT GCTCATCGGC CCTGCCAAGA TCTTCTCCAG CGTCCAGTGG 
CTCTCGGGGG TCAAGCCGGT GCTCAGCACC AAGAGCAAGT TCCGCGTGGT GGTGGAAGAG 
GTGCAGGTGG TGGAGCTGAA GGTGACGTGG ATTACCAAGA GCTTCTGCCC AGGGGGCACA 
GACAGCGTCA GCCCCCCGCC CTCTGTCATC ACCCAGGAAA ACCTAGGCAG GGTGAAGCGT 
CTCGGATGCT TTGACCATGC TCAGCGGCAG CTGGGGGAGC GCTGTCTTTA TGTCTTCCCA 
GCCAAGGTAG AGCCGGCCAG GATTGCCTGT GAATGTCCAG AAAAACACTG CGCCCAGGGG 
GAGGGCTCTA TGGCCAAGAA GGTGAAGCGC CTCTTGAAGA AGCAGGTCGT GAGGATCATG 
TCGTGCTCCC CGGACACCCC GTGCCCCCGG GACCATTCCG TGGAGGACGC GGCGGGCAAG 
AAGGGGGGCG CCAAAGCCAA GAGCGAGACG GGGTCCACCA GCCCCGAGGG CACGCCCGGC 
GGGTCCGCCA GCCCCGAGGA GATGCAGGAC GAGGGCCCGG AGGAGGCACA GGAGGCCGGC 
GAGCAGCTGC CCCCCTTCCT GCTGAAGGAA GCGGGGGACG ACAGGCTGCA CTCAGCCGAG 
CAGGACGCGG ATGATGAGGC CGCCGACGAC ACGGATGACA CCAGCTCCGT GACCTCCTCC 
GCCAGCTCCA CCACCTCCTC CCAGAGTGGC AGTGGCGCAA GTCGCAAAAA GAGCATACCC 
TTGTCTATCA AGAACCTAAA GCGGAAGCAC AAGCGGAAGA AGAATAAAAT CACTCGCGAC 
TTCAAGCCAG GGGACAGGGT GGCGGTGGAG GTGGTGACCA CAATGACCTC GGCGGACGTG 
ATGTGGCAGG ACGGCTCTGT GGAGTGCAGC ATCCGCTCCA ACGACCTCTT CCCCGTGCAC 
CACCTGGACA ACAACGAGTT CTGCCCCGGG GACTTCGTGG TGGACAAGCG AGTCCAGAGC 
TGCCCGGATC CCGCTGTCTA TGGCGTGGTG CAGTCCGGGG ACCACGTGGG CCGCACCTGC 
ATGGTCAAGT GGTTCAAGCT GCGGCCGAGC GGAGACGACG TGGAGCTGAT CGGGGAAGAG 
GAAGACGTGA GCGTCTACGA CATCGCTGAC CACCCTGACT TTCGGTTCCG CACAACCGAC 
ATCGTCATCC GAATTGGCAA CACGGAGGAT GGAGCACCTC ACAAGGAGGA TGAGCCGTCG 
GTCGGCCAGG TGGCCCGCGT GGACGTCAGC AGCAAGGTGG AGGTGGTGTG GGCTGACAAC 
TCCAAGACCA TCATCCTGCC GCAGCACCTG TACAACATCG AGTCCGAGAT CGAGGAGTCA 
GACTATGACT CGGTCGAAGG CAGCACCAGC GGGGCATCCT CGGATGAGTG GGAGGACGAC 
AGTGACAGCT GGGAGACGGA CAATGGGCTG GTGGAGGACG AGCACCCCAA GATAGAGGAG 
CCCCCCCTCC CAGCCACCGA GCCGCCGGCA GCCCCCGAGG AGGACAAGGG TGTGGTGATC 
AGCGAGGAGG CCGCCACGGC CGCCATCCAG GGGGCCGTGG CCATGGCCGC CCCTGTGGCC 
GGGCTGATGG AGAAGGCTGG CAAGGACGGG CCCCCGAAGA GCTTCCGCGA GCTGAAGGAG 
GCCATCAAGA TCCTGGAGAG CCTCAAGAAC ATGACCGTGG AGCAGCTCCT GACGGGCTCG 
CCCACCTCCC CCACAGTGGA GCCCGAGAAG CCCACGCGGG AGAAGAAGTT TCTGGATGAC 
ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG ACCCTGGACA ACGTGGCCAT CGCGGAGGAG 
GAGAAGATGG AGGCGGCGCC GGACGCGGAG CGCAAGGAGG AGAAGCCCGA GGGGCAGTCG 
CCCGTGAAGG CCGAGTGGCC CAGCGAGACG CCCGTGCTCT GCCAGCAGTG CGGCGGCAAG 
CCCGGGGTCA CCTTCACCAG CGCCAAGGGC GAGGTCTTCT CGGTGCTGGA GTTCGCGCCC 
TCAAATCACT CTTTTAAGAA GATTGAGTTC CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC 
ACAGTGCGGA AGGAGATGGC GCTGCTGGCC ACCTCGCTGC CCGATGGCAT CATGGTCAAG 
ACCTTTGAGG ACAGGATGGA CCTCTTCTCG GCCCTGATCA AAGGCCCCAC TCGCACCCCG 
TACGAGGACG GCCTCTACCT GTTTGACATC CAGCTCCCCA ACATCTACCC GGCCGTGCCC 
CCCCACTTCT GCTACCTCTC CCAGTGCAGC GGCCGCCTGA ACCCCAACCT GTATGACAAC 
GGGAAGGTGT GCGTCAGCCT CCTGGGCACC TGGATTGGAA AGGGGACAGA GAGGTGGACG 
AGCAAATCCA GCCTTCTCCA GGTGCTCATC TCCATCCAAG GTCTCATCCT GGTGAATGAG 
CCCTATTACA ACGAAGCTGG CTTCGACAGC GACCGCGGCC TGCAGGAGGG CTACGAAAAC 
AGTCGCTGCT ACAACGAGAT GGCGCTCATC CGAGTCGTGC AGTCCATGAC CCAGCTGGTG 
CGGCGGCCGC CCGAGGTCTT CGAGCAGGAG ATCCGGCAGC ACTTCAGTAC GGGCGGCTGG 
CGGCTGGTGA GCCGCATCGA GTCCTGGCTG GAGACCCACG CCCTGCTGGA GAGGGCCCAG 
GCGCTGCCCA ACGGGCTCCC CAAGGACAGC AGCTCGCCCG AGCCGCCTGC TGCGGCCGAG 
CTCTCAGACT TCGGCCGAGA AGAACCTGAG GACGGAGGGC TGGCCCCTGG AGAGGCCTCC 
CAGGGCTCAG ACTCGGAGGG CGGCGCCCAA GGCCTGGCCT CAGCTGGCAG GGACCGCACA 
GACCAGACTT CGGAGGCAGC GCCTGACACC GCGGTGCCAC CCAGTGTCAA ACCAAAGAAG 
CGGAGAAAGA GCTACCGGAG CTTCTTGCCT GAGAAGAGTG GCTACCCTGA CATCGGCTTC 
CCCCTCTTCC CGCTTTCCAA GGGTTTCATC AAGAGCATCC GGGGGGTCCT GACGCAGTTC 
CGGGCCGCCC TGATAGAGGC GGGCATGCCC CAGGGCGCCG AGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020922245.1
CDS	54..3944
Translation	MADPAAPAPAAPAPAQAPDAAPDAAPALDAVPAPAPAPAPAPDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVMDVSIDCAVKLIGTNCIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTPCPRDHSVEDAAGKKGGAKAKSETGSTSPEGTPGGSASPEEMQDEGPEEAQEAGEQLPPFLLKEAGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGASRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPLPATEPPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDAERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHALLERAQALPNGLPKDSSSPEPPAAAELSDFGREEPEDGGLAPGEASQGSDSEGGAQGLASAGRDRTDQTSEAAPDTAVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALIEAGMPQGAEDK

Target ORF information:

RefSeq Version	XM_021066586.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021066586.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGCC AGCGCCCGCA GCCCCGGCTC CCGCCCAGGC CCCGGACGCG 
GCTCCAGACG CGGCCCCGGC CCTGGACGCA GTCCCCGCCC CGGCCCCGGC GCCAGCGCCC 
GCCCCGGACT CGGCCTCGGG GCCGTCTTCG GACTCCGGCC CCGAAGCCGG CTCGCAGCGG 
CTGCTGTTCT CTCACGACCT GGTGTCGGGC CGTTACCGCG GCTCGGTGCA CTTCGGGCTG 
GTGCGCCTCA TCCACGGCGA GGACTCGGAC TCAGAGGGCG AGGAGGAGGG CCGCGGGAGC 
TCGGGCTGCT CCGAGGCAGG GGGCGCGGGC CACGAGGAGG GCCGGGCCAG CCCGCTGCGC 
CGCGGCTACG TGCGCGTCCA GTGGTACCCG GAGGGCGTCA AGCAGCACGT GAAGGAGACC 
AAGCTGAAGC TGGAGGACCG CTCCGTGGTG CCCCGCGACG TGGTCCGGCA CATGAGATCC 
ACTGACAGCC AGTGCGGCAC TGTGATGGAC GTCAGCATCG ACTGCGCCGT CAAGCTCATC 
GGCACCAACT GCATCATCTA CCCCGTCAAC AGCCGGGACC TCCAGCACAT CTGGCCCTTC 
ATGTATGGGG ACTACATCGC CTACGACTGC TGGCTGGGGA AGGTCTACGA CCTGAAGAAC 
CAGATCATCC TCAAGCTGTC CAACGGCGCC AGGTGCTCCA TGAACACAGA AGACGGCGCT 
AAGCTCTACG ACGTCTGCCC GCATGTCAGC GACTCTGGCC TCTTCTTCGA TGACTCCTAC 
GGCTTCTACC CAGGCCAGGT GCTCATCGGC CCTGCCAAGA TCTTCTCCAG CGTCCAGTGG 
CTCTCGGGGG TCAAGCCGGT GCTCAGCACC AAGAGCAAGT TCCGCGTGGT GGTGGAAGAG 
GTGCAGGTGG TGGAGCTGAA GGTGACGTGG ATTACCAAGA GCTTCTGCCC AGGGGGCACA 
GACAGCGTCA GCCCCCCGCC CTCTGTCATC ACCCAGGAAA ACCTAGGCAG GGTGAAGCGT 
CTCGGATGCT TTGACCATGC TCAGCGGCAG CTGGGGGAGC GCTGTCTTTA TGTCTTCCCA 
GCCAAGGTAG AGCCGGCCAG GATTGCCTGT GAATGTCCAG AAAAACACTG CGCCCAGGGG 
GAGGGCTCTA TGGCCAAGAA GGTGAAGCGC CTCTTGAAGA AGCAGGTCGT GAGGATCATG 
TCGTGCTCCC CGGACACCCC GTGCCCCCGG GACCATTCCG TGGAGGACGC GGCGGGCAAG 
AAGGGGGGCG CCAAAGCCAA GAGCGAGACG GGGTCCACCA GCCCCGAGGG CACGCCCGGC 
GGGTCCGCCA GCCCCGAGGA GATGCAGGAC GAGGGCCCGG AGGAGGCACA GGAGGCCGGC 
GAGCAGCTGC CCCCCTTCCT GCTGAAGGAA GCGGGGGACG ACAGGCTGCA CTCAGCCGAG 
CAGGACGCGG ATGATGAGGC CGCCGACGAC ACGGATGACA CCAGCTCCGT GACCTCCTCC 
GCCAGCTCCA CCACCTCCTC CCAGAGTGGC AGTGGCGCAA GTCGCAAAAA GAGCATACCC 
TTGTCTATCA AGAACCTAAA GCGGAAGCAC AAGCGGAAGA AGAATAAAAT CACTCGCGAC 
TTCAAGCCAG GGGACAGGGT GGCGGTGGAG GTGGTGACCA CAATGACCTC GGCGGACGTG 
ATGTGGCAGG ACGGCTCTGT GGAGTGCAGC ATCCGCTCCA ACGACCTCTT CCCCGTGCAC 
CACCTGGACA ACAACGAGTT CTGCCCCGGG GACTTCGTGG TGGACAAGCG AGTCCAGAGC 
TGCCCGGATC CCGCTGTCTA TGGCGTGGTG CAGTCCGGGG ACCACGTGGG CCGCACCTGC 
ATGGTCAAGT GGTTCAAGCT GCGGCCGAGC GGAGACGACG TGGAGCTGAT CGGGGAAGAG 
GAAGACGTGA GCGTCTACGA CATCGCTGAC CACCCTGACT TTCGGTTCCG CACAACCGAC 
ATCGTCATCC GAATTGGCAA CACGGAGGAT GGAGCACCTC ACAAGGAGGA TGAGCCGTCG 
GTCGGCCAGG TGGCCCGCGT GGACGTCAGC AGCAAGGTGG AGGTGGTGTG GGCTGACAAC 
TCCAAGACCA TCATCCTGCC GCAGCACCTG TACAACATCG AGTCCGAGAT CGAGGAGTCA 
GACTATGACT CGGTCGAAGG CAGCACCAGC GGGGCATCCT CGGATGAGTG GGAGGACGAC 
AGTGACAGCT GGGAGACGGA CAATGGGCTG GTGGAGGACG AGCACCCCAA GATAGAGGAG 
CCCCCCCTCC CAGCCACCGA GCCGCCGGCA GCCCCCGAGG AGGACAAGGG TGTGGTGATC 
AGCGAGGAGG CCGCCACGGC CGCCATCCAG GGGGCCGTGG CCATGGCCGC CCCTGTGGCC 
GGGCTGATGG AGAAGGCTGG CAAGGACGGG CCCCCGAAGA GCTTCCGCGA GCTGAAGGAG 
GCCATCAAGA TCCTGGAGAG CCTCAAGAAC ATGACCGTGG AGCAGCTCCT GACGGGCTCG 
CCCACCTCCC CCACAGTGGA GCCCGAGAAG CCCACGCGGG AGAAGAAGTT TCTGGATGAC 
ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG ACCCTGGACA ACGTGGCCAT CGCGGAGGAG 
GAGAAGATGG AGGCGGCGCC GGACGCGGAG CGCAAGGAGG AGAAGCCCGA GGGGCAGTCG 
CCCGTGAAGG CCGAGTGGCC CAGCGAGACG CCCGTGCTCT GCCAGCAGTG CGGCGGCAAG 
CCCGGGGTCA CCTTCACCAG CGCCAAGGGC GAGGTCTTCT CGGTGCTGGA GTTCGCGCCC 
TCAAATCACT CTTTTAAGAA GATTGAGTTC CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC 
ACAGTGCGGA AGGAGATGGC GCTGCTGGCC ACCTCGCTGC CCGATGGCAT CATGGTCAAG 
ACCTTTGAGG ACAGGATGGA CCTCTTCTCG GCCCTGATCA AAGGCCCCAC TCGCACCCCG 
TACGAGGACG GCCTCTACCT GTTTGACATC CAGCTCCCCA ACATCTACCC GGCCGTGCCC 
CCCCACTTCT GCTACCTCTC CCAGTGCAGC GGCCGCCTGA ACCCCAACCT GTATGACAAC 
GGGAAGGTGT GCGTCAGCCT CCTGGGCACC TGGATTGGAA AGGGGACAGA GAGGTGGACG 
AGCAAATCCA GCCTTCTCCA GGTGCTCATC TCCATCCAAG GTCTCATCCT GGTGAATGAG 
CCCTATTACA ACGAAGCTGG CTTCGACAGC GACCGCGGCC TGCAGGAGGG CTACGAAAAC 
AGTCGCTGCT ACAACGAGAT GGCGCTCATC CGAGTCGTGC AGTCCATGAC CCAGCTGGTG 
CGGCGGCCGC CCGAGGTCTT CGAGCAGGAG ATCCGGCAGC ACTTCAGTAC GGGCGGCTGG 
CGGCTGGTGA GCCGCATCGA GTCCTGGCTG GAGACCCACG CCCTGCTGGA GAGGGCCCAG 
GCGCTGCCCA ACGGGCTCCC CAAGGACAGC AGCTCGCCCG AGCCGCCTGC TGCGGCCGAG 
CTCTCAGACT TCGGCCGAGA AGAACCTGAG GACGGAGGGC TGGCCCCTGG AGAGGCCTCC 
CAGGGCTCAG ACTCGGAGGG CGGCGCCCAA GGCCTGGCCT CAGCTGGCAG GGACCGCACA 
GACCAGACTT CGGAGGCAGC GCCTGACACC GCGGTGCCAC CCAGTGTCAA ACCAAAGAAG 
CGGAGAAAGA GCTACCGGAG CTTCTTGCCT GAGAAGAGTG GCTACCCTGA CATCGGCTTC 
CCCCTCTTCC CGCTTTCCAA GGGTTTCATC AAGAGCATCC GGGGGGTCCT GACGCAGTTC 
CGGGCCGCCC TGATAGAGGC GGGCATGCCC CAGGGCGCCG AGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_021066587.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020922246.1 Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe233511
Clone ID Related Accession (Same CDS sequence)	XM_021066587.1
Accession Version	XM_021066587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010454.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGCC AGCGCCCGCA GCCCCGGCTC CCGCCCAGGC CCCGGACGCG 
GCTCCAGACG CGGCCCCGGC CCTGGACGCA GTCCCCGCCC CGGCCCCGGC GCCAGCGCCC 
GCCCCGGACT CGGCCTCGGG GCCGTCTTCG GACTCCGGCC CCGAAGCCGG CTCGCAGCGG 
CTGCTGTTCT CTCACGACCT GGTGTCGGGC CGTTACCGCG GCTCGGTGCA CTTCGGGCTG 
GTGCGCCTCA TCCACGGCGA GGACTCGGAC TCAGAGGGCG AGGAGGAGGG CCGCGGGAGC 
TCGGGCTGCT CCGAGGCAGG GGGCGCGGGC CACGAGGAGG GCCGGGCCAG CCCGCTGCGC 
CGCGGCTACG TGCGCGTCCA GTGGTACCCG GAGGGCGTCA AGCAGCACGT GAAGGAGACC 
AAGCTGAAGC TGGAGGACCG CTCCGTGGTG CCCCGCGACG TGGTCCGGCA CATGAGATCC 
ACTGACAGCC AGTGCGGCAC TGTGATGGAC GTCAGCATCG ACTGCGCCGT CAAGCTCATC 
GGCACCAACT GCATCATCTA CCCCGTCAAC AGCCGGGACC TCCAGCACAT CTGGCCCTTC 
ATGTATGGGG ACTACATCGC CTACGACTGC TGGCTGGGGA AGGTCTACGA CCTGAAGAAC 
CAGATCATCC TCAAGCTGTC CAACGGCGCC AGGTGCTCCA TGAACACAGA AGACGGCGCT 
AAGCTCTACG ACGTCTGCCC GCATGTCAGC GACTCTGGCC TCTTCTTCGA TGACTCCTAC 
GGCTTCTACC CAGGCCAGGT GCTCATCGGC CCTGCCAAGA TCTTCTCCAG CGTCCAGTGG 
CTCTCGGGGG TCAAGCCGGT GCTCAGCACC AAGAGCAAGT TCCGCGTGGT GGTGGAAGAG 
GTGCAGGTGG TGGAGCTGAA GGTGACGTGG ATTACCAAGA GCTTCTGCCC AGGGGGCACA 
GACAGCGTCA GCCCCCCGCC CTCTGTCATC ACCCAGGAAA ACCTAGGCAG GGTGAAGCGT 
CTCGGATGCT TTGACCATGC TCAGCGGCAG CTGGGGGAGC GCTGTCTTTA TGTCTTCCCA 
GCCAAGGTGA AGCGCCTCTT GAAGAAGCAG GTCGTGAGGA TCATGTCGTG CTCCCCGGAC 
ACCCCGTGCC CCCGGGACCA TTCCGTGGAG GACGCGGCGG GCAAGAAGGG GGGCGCCAAA 
GCCAAGAGCG AGACGGGGTC CACCAGCCCC GAGGGCACGC CCGGCGGGTC CGCCAGCCCC 
GAGGAGATGC AGGACGAGGG CCCGGAGGAG GCACAGGAGG CCGGCGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAAGCGGG GGACGACAGG CTGCACTCAG CCGAGCAGGA CGCGGATGAT 
GAGGCCGCCG ACGACACGGA TGACACCAGC TCCGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GTGGCAGTGG CGCAAGTCGC AAAAAGAGCA TACCCTTGTC TATCAAGAAC 
CTAAAGCGGA AGCACAAGCG GAAGAAGAAT AAAATCACTC GCGACTTCAA GCCAGGGGAC 
AGGGTGGCGG TGGAGGTGGT GACCACAATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCTGTGGAGT GCAGCATCCG CTCCAACGAC CTCTTCCCCG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGGGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGCCC GGATCCCGCT 
GTCTATGGCG TGGTGCAGTC CGGGGACCAC GTGGGCCGCA CCTGCATGGT CAAGTGGTTC 
AAGCTGCGGC CGAGCGGAGA CGACGTGGAG CTGATCGGGG AAGAGGAAGA CGTGAGCGTC 
TACGACATCG CTGACCACCC TGACTTTCGG TTCCGCACAA CCGACATCGT CATCCGAATT 
GGCAACACGG AGGATGGAGC ACCTCACAAG GAGGATGAGC CGTCGGTCGG CCAGGTGGCC 
CGCGTGGACG TCAGCAGCAA GGTGGAGGTG GTGTGGGCTG ACAACTCCAA GACCATCATC 
CTGCCGCAGC ACCTGTACAA CATCGAGTCC GAGATCGAGG AGTCAGACTA TGACTCGGTC 
GAAGGCAGCA CCAGCGGGGC ATCCTCGGAT GAGTGGGAGG ACGACAGTGA CAGCTGGGAG 
ACGGACAATG GGCTGGTGGA GGACGAGCAC CCCAAGATAG AGGAGCCCCC CCTCCCAGCC 
ACCGAGCCGC CGGCAGCCCC CGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCCGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCTG TGGCCGGGCT GATGGAGAAG 
GCTGGCAAGG ACGGGCCCCC GAAGAGCTTC CGCGAGCTGA AGGAGGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC CGTGGAGCAG CTCCTGACGG GCTCGCCCAC CTCCCCCACA 
GTGGAGCCCG AGAAGCCCAC GCGGGAGAAG AAGTTTCTGG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAACGTG GCCATCGCGG AGGAGGAGAA GATGGAGGCG 
GCGCCGGACG CGGAGCGCAA GGAGGAGAAG CCCGAGGGGC AGTCGCCCGT GAAGGCCGAG 
TGGCCCAGCG AGACGCCCGT GCTCTGCCAG CAGTGCGGCG GCAAGCCCGG GGTCACCTTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CGCCCTCAAA TCACTCTTTT 
AAGAAGATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCGCTGC TGGCCACCTC GCTGCCCGAT GGCATCATGG TCAAGACCTT TGAGGACAGG 
ATGGACCTCT TCTCGGCCCT GATCAAAGGC CCCACTCGCA CCCCGTACGA GGACGGCCTC 
TACCTGTTTG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGCGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TCATCTCCAT CCAAGGTCTC ATCCTGGTGA ATGAGCCCTA TTACAACGAA 
GCTGGCTTCG ACAGCGACCG CGGCCTGCAG GAGGGCTACG AAAACAGTCG CTGCTACAAC 
GAGATGGCGC TCATCCGAGT CGTGCAGTCC ATGACCCAGC TGGTGCGGCG GCCGCCCGAG 
GTCTTCGAGC AGGAGATCCG GCAGCACTTC AGTACGGGCG GCTGGCGGCT GGTGAGCCGC 
ATCGAGTCCT GGCTGGAGAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCCGAGCCG CCTGCTGCGG CCGAGCTCTC AGACTTCGGC 
CGAGAAGAAC CTGAGGACGG AGGGCTGGCC CCTGGAGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCGGCG CCCAAGGCCT GGCCTCAGCT GGCAGGGACC GCACAGACCA GACTTCGGAG 
GCAGCGCCTG ACACCGCGGT GCCACCCAGT GTCAAACCAA AGAAGCGGAG AAAGAGCTAC 
CGGAGCTTCT TGCCTGAGAA GAGTGGCTAC CCTGACATCG GCTTCCCCCT CTTCCCGCTT 
TCCAAGGGTT TCATCAAGAG CATCCGGGGG GTCCTGACGC AGTTCCGGGC CGCCCTGATA 
GAGGCGGGCA TGCCCCAGGG CGCCGAGGAC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020922246.1
CDS	54..3869
Translation	MADPAAPAPAAPAPAQAPDAAPDAAPALDAVPAPAPAPAPAPDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVMDVSIDCAVKLIGTNCIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCSPDTPCPRDHSVEDAAGKKGGAKAKSETGSTSPEGTPGGSASPEEMQDEGPEEAQEAGEQLPPFLLKEAGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGASRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPLPATEPPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDAERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHALLERAQALPNGLPKDSSSPEPPAAAELSDFGREEPEDGGLAPGEASQGSDSEGGAQGLASAGRDRTDQTSEAAPDTAVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALIEAGMPQGAEDK

Target ORF information:

RefSeq Version	XM_021066587.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021066587.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGCC AGCGCCCGCA GCCCCGGCTC CCGCCCAGGC CCCGGACGCG 
GCTCCAGACG CGGCCCCGGC CCTGGACGCA GTCCCCGCCC CGGCCCCGGC GCCAGCGCCC 
GCCCCGGACT CGGCCTCGGG GCCGTCTTCG GACTCCGGCC CCGAAGCCGG CTCGCAGCGG 
CTGCTGTTCT CTCACGACCT GGTGTCGGGC CGTTACCGCG GCTCGGTGCA CTTCGGGCTG 
GTGCGCCTCA TCCACGGCGA GGACTCGGAC TCAGAGGGCG AGGAGGAGGG CCGCGGGAGC 
TCGGGCTGCT CCGAGGCAGG GGGCGCGGGC CACGAGGAGG GCCGGGCCAG CCCGCTGCGC 
CGCGGCTACG TGCGCGTCCA GTGGTACCCG GAGGGCGTCA AGCAGCACGT GAAGGAGACC 
AAGCTGAAGC TGGAGGACCG CTCCGTGGTG CCCCGCGACG TGGTCCGGCA CATGAGATCC 
ACTGACAGCC AGTGCGGCAC TGTGATGGAC GTCAGCATCG ACTGCGCCGT CAAGCTCATC 
GGCACCAACT GCATCATCTA CCCCGTCAAC AGCCGGGACC TCCAGCACAT CTGGCCCTTC 
ATGTATGGGG ACTACATCGC CTACGACTGC TGGCTGGGGA AGGTCTACGA CCTGAAGAAC 
CAGATCATCC TCAAGCTGTC CAACGGCGCC AGGTGCTCCA TGAACACAGA AGACGGCGCT 
AAGCTCTACG ACGTCTGCCC GCATGTCAGC GACTCTGGCC TCTTCTTCGA TGACTCCTAC 
GGCTTCTACC CAGGCCAGGT GCTCATCGGC CCTGCCAAGA TCTTCTCCAG CGTCCAGTGG 
CTCTCGGGGG TCAAGCCGGT GCTCAGCACC AAGAGCAAGT TCCGCGTGGT GGTGGAAGAG 
GTGCAGGTGG TGGAGCTGAA GGTGACGTGG ATTACCAAGA GCTTCTGCCC AGGGGGCACA 
GACAGCGTCA GCCCCCCGCC CTCTGTCATC ACCCAGGAAA ACCTAGGCAG GGTGAAGCGT 
CTCGGATGCT TTGACCATGC TCAGCGGCAG CTGGGGGAGC GCTGTCTTTA TGTCTTCCCA 
GCCAAGGTGA AGCGCCTCTT GAAGAAGCAG GTCGTGAGGA TCATGTCGTG CTCCCCGGAC 
ACCCCGTGCC CCCGGGACCA TTCCGTGGAG GACGCGGCGG GCAAGAAGGG GGGCGCCAAA 
GCCAAGAGCG AGACGGGGTC CACCAGCCCC GAGGGCACGC CCGGCGGGTC CGCCAGCCCC 
GAGGAGATGC AGGACGAGGG CCCGGAGGAG GCACAGGAGG CCGGCGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAAGCGGG GGACGACAGG CTGCACTCAG CCGAGCAGGA CGCGGATGAT 
GAGGCCGCCG ACGACACGGA TGACACCAGC TCCGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GTGGCAGTGG CGCAAGTCGC AAAAAGAGCA TACCCTTGTC TATCAAGAAC 
CTAAAGCGGA AGCACAAGCG GAAGAAGAAT AAAATCACTC GCGACTTCAA GCCAGGGGAC 
AGGGTGGCGG TGGAGGTGGT GACCACAATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCTGTGGAGT GCAGCATCCG CTCCAACGAC CTCTTCCCCG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGGGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGCCC GGATCCCGCT 
GTCTATGGCG TGGTGCAGTC CGGGGACCAC GTGGGCCGCA CCTGCATGGT CAAGTGGTTC 
AAGCTGCGGC CGAGCGGAGA CGACGTGGAG CTGATCGGGG AAGAGGAAGA CGTGAGCGTC 
TACGACATCG CTGACCACCC TGACTTTCGG TTCCGCACAA CCGACATCGT CATCCGAATT 
GGCAACACGG AGGATGGAGC ACCTCACAAG GAGGATGAGC CGTCGGTCGG CCAGGTGGCC 
CGCGTGGACG TCAGCAGCAA GGTGGAGGTG GTGTGGGCTG ACAACTCCAA GACCATCATC 
CTGCCGCAGC ACCTGTACAA CATCGAGTCC GAGATCGAGG AGTCAGACTA TGACTCGGTC 
GAAGGCAGCA CCAGCGGGGC ATCCTCGGAT GAGTGGGAGG ACGACAGTGA CAGCTGGGAG 
ACGGACAATG GGCTGGTGGA GGACGAGCAC CCCAAGATAG AGGAGCCCCC CCTCCCAGCC 
ACCGAGCCGC CGGCAGCCCC CGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCCGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCTG TGGCCGGGCT GATGGAGAAG 
GCTGGCAAGG ACGGGCCCCC GAAGAGCTTC CGCGAGCTGA AGGAGGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC CGTGGAGCAG CTCCTGACGG GCTCGCCCAC CTCCCCCACA 
GTGGAGCCCG AGAAGCCCAC GCGGGAGAAG AAGTTTCTGG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAACGTG GCCATCGCGG AGGAGGAGAA GATGGAGGCG 
GCGCCGGACG CGGAGCGCAA GGAGGAGAAG CCCGAGGGGC AGTCGCCCGT GAAGGCCGAG 
TGGCCCAGCG AGACGCCCGT GCTCTGCCAG CAGTGCGGCG GCAAGCCCGG GGTCACCTTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CGCCCTCAAA TCACTCTTTT 
AAGAAGATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCGCTGC TGGCCACCTC GCTGCCCGAT GGCATCATGG TCAAGACCTT TGAGGACAGG 
ATGGACCTCT TCTCGGCCCT GATCAAAGGC CCCACTCGCA CCCCGTACGA GGACGGCCTC 
TACCTGTTTG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGCGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TCATCTCCAT CCAAGGTCTC ATCCTGGTGA ATGAGCCCTA TTACAACGAA 
GCTGGCTTCG ACAGCGACCG CGGCCTGCAG GAGGGCTACG AAAACAGTCG CTGCTACAAC 
GAGATGGCGC TCATCCGAGT CGTGCAGTCC ATGACCCAGC TGGTGCGGCG GCCGCCCGAG 
GTCTTCGAGC AGGAGATCCG GCAGCACTTC AGTACGGGCG GCTGGCGGCT GGTGAGCCGC 
ATCGAGTCCT GGCTGGAGAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCCGAGCCG CCTGCTGCGG CCGAGCTCTC AGACTTCGGC 
CGAGAAGAAC CTGAGGACGG AGGGCTGGCC CCTGGAGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCGGCG CCCAAGGCCT GGCCTCAGCT GGCAGGGACC GCACAGACCA GACTTCGGAG 
GCAGCGCCTG ACACCGCGGT GCCACCCAGT GTCAAACCAA AGAAGCGGAG AAAGAGCTAC 
CGGAGCTTCT TGCCTGAGAA GAGTGGCTAC CCTGACATCG GCTTCCCCCT CTTCCCGCTT 
TCCAAGGGTT TCATCAAGAG CATCCGGGGG GTCCTGACGC AGTTCCGGGC CGCCCTGATA 
GAGGCGGGCA TGCCCCAGGG CGCCGAGGAC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_021066588.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020922247.1 Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

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CloneID	OSe233512
Clone ID Related Accession (Same CDS sequence)	XM_021066588.1
Accession Version	XM_021066588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010454.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCCA CTGACAGCCA GTGCGGCACT GTGATGGACG TCAGCATCGA CTGCGCCGTC 
AAGCTCATCG GCACCAACTG CATCATCTAC CCCGTCAACA GCCGGGACCT CCAGCACATC 
TGGCCCTTCA TGTATGGGGA CTACATCGCC TACGACTGCT GGCTGGGGAA GGTCTACGAC 
CTGAAGAACC AGATCATCCT CAAGCTGTCC AACGGCGCCA GGTGCTCCAT GAACACAGAA 
GACGGCGCTA AGCTCTACGA CGTCTGCCCG CATGTCAGCG ACTCTGGCCT CTTCTTCGAT 
GACTCCTACG GCTTCTACCC AGGCCAGGTG CTCATCGGCC CTGCCAAGAT CTTCTCCAGC 
GTCCAGTGGC TCTCGGGGGT CAAGCCGGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG 
GTGGAAGAGG TGCAGGTGGT GGAGCTGAAG GTGACGTGGA TTACCAAGAG CTTCTGCCCA 
GGGGGCACAG ACAGCGTCAG CCCCCCGCCC TCTGTCATCA CCCAGGAAAA CCTAGGCAGG 
GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TGGGGGAGCG CTGTCTTTAT 
GTCTTCCCAG CCAAGGTAGA GCCGGCCAGG ATTGCCTGTG AATGTCCAGA AAAACACTGC 
GCCCAGGGGG AGGGCTCTAT GGCCAAGAAG GTGAAGCGCC TCTTGAAGAA GCAGGTCGTG 
AGGATCATGT CGTGCTCCCC GGACACCCCG TGCCCCCGGG ACCATTCCGT GGAGGACGCG 
GCGGGCAAGA AGGGGGGCGC CAAAGCCAAG AGCGAGACGG GGTCCACCAG CCCCGAGGGC 
ACGCCCGGCG GGTCCGCCAG CCCCGAGGAG ATGCAGGACG AGGGCCCGGA GGAGGCACAG 
GAGGCCGGCG AGCAGCTGCC CCCCTTCCTG CTGAAGGAAG CGGGGGACGA CAGGCTGCAC 
TCAGCCGAGC AGGACGCGGA TGATGAGGCC GCCGACGACA CGGATGACAC CAGCTCCGTG 
ACCTCCTCCG CCAGCTCCAC CACCTCCTCC CAGAGTGGCA GTGGCGCAAG TCGCAAAAAG 
AGCATACCCT TGTCTATCAA GAACCTAAAG CGGAAGCACA AGCGGAAGAA GAATAAAATC 
ACTCGCGACT TCAAGCCAGG GGACAGGGTG GCGGTGGAGG TGGTGACCAC AATGACCTCG 
GCGGACGTGA TGTGGCAGGA CGGCTCTGTG GAGTGCAGCA TCCGCTCCAA CGACCTCTTC 
CCCGTGCACC ACCTGGACAA CAACGAGTTC TGCCCCGGGG ACTTCGTGGT GGACAAGCGA 
GTCCAGAGCT GCCCGGATCC CGCTGTCTAT GGCGTGGTGC AGTCCGGGGA CCACGTGGGC 
CGCACCTGCA TGGTCAAGTG GTTCAAGCTG CGGCCGAGCG GAGACGACGT GGAGCTGATC 
GGGGAAGAGG AAGACGTGAG CGTCTACGAC ATCGCTGACC ACCCTGACTT TCGGTTCCGC 
ACAACCGACA TCGTCATCCG AATTGGCAAC ACGGAGGATG GAGCACCTCA CAAGGAGGAT 
GAGCCGTCGG TCGGCCAGGT GGCCCGCGTG GACGTCAGCA GCAAGGTGGA GGTGGTGTGG 
GCTGACAACT CCAAGACCAT CATCCTGCCG CAGCACCTGT ACAACATCGA GTCCGAGATC 
GAGGAGTCAG ACTATGACTC GGTCGAAGGC AGCACCAGCG GGGCATCCTC GGATGAGTGG 
GAGGACGACA GTGACAGCTG GGAGACGGAC AATGGGCTGG TGGAGGACGA GCACCCCAAG 
ATAGAGGAGC CCCCCCTCCC AGCCACCGAG CCGCCGGCAG CCCCCGAGGA GGACAAGGGT 
GTGGTGATCA GCGAGGAGGC CGCCACGGCC GCCATCCAGG GGGCCGTGGC CATGGCCGCC 
CCTGTGGCCG GGCTGATGGA GAAGGCTGGC AAGGACGGGC CCCCGAAGAG CTTCCGCGAG 
CTGAAGGAGG CCATCAAGAT CCTGGAGAGC CTCAAGAACA TGACCGTGGA GCAGCTCCTG 
ACGGGCTCGC CCACCTCCCC CACAGTGGAG CCCGAGAAGC CCACGCGGGA GAAGAAGTTT 
CTGGATGACA TCAAGAAGCT GCAGGAGAAC CTCAAGAAGA CCCTGGACAA CGTGGCCATC 
GCGGAGGAGG AGAAGATGGA GGCGGCGCCG GACGCGGAGC GCAAGGAGGA GAAGCCCGAG 
GGGCAGTCGC CCGTGAAGGC CGAGTGGCCC AGCGAGACGC CCGTGCTCTG CCAGCAGTGC 
GGCGGCAAGC CCGGGGTCAC CTTCACCAGC GCCAAGGGCG AGGTCTTCTC GGTGCTGGAG 
TTCGCGCCCT CAAATCACTC TTTTAAGAAG ATTGAGTTCC AGCCTCCAGA AGCCAAGAAG 
TTCTTCAGCA CAGTGCGGAA GGAGATGGCG CTGCTGGCCA CCTCGCTGCC CGATGGCATC 
ATGGTCAAGA CCTTTGAGGA CAGGATGGAC CTCTTCTCGG CCCTGATCAA AGGCCCCACT 
CGCACCCCGT ACGAGGACGG CCTCTACCTG TTTGACATCC AGCTCCCCAA CATCTACCCG 
GCCGTGCCCC CCCACTTCTG CTACCTCTCC CAGTGCAGCG GCCGCCTGAA CCCCAACCTG 
TATGACAACG GGAAGGTGTG CGTCAGCCTC CTGGGCACCT GGATTGGAAA GGGGACAGAG 
AGGTGGACGA GCAAATCCAG CCTTCTCCAG GTGCTCATCT CCATCCAAGG TCTCATCCTG 
GTGAATGAGC CCTATTACAA CGAAGCTGGC TTCGACAGCG ACCGCGGCCT GCAGGAGGGC 
TACGAAAACA GTCGCTGCTA CAACGAGATG GCGCTCATCC GAGTCGTGCA GTCCATGACC 
CAGCTGGTGC GGCGGCCGCC CGAGGTCTTC GAGCAGGAGA TCCGGCAGCA CTTCAGTACG 
GGCGGCTGGC GGCTGGTGAG CCGCATCGAG TCCTGGCTGG AGACCCACGC CCTGCTGGAG 
AGGGCCCAGG CGCTGCCCAA CGGGCTCCCC AAGGACAGCA GCTCGCCCGA GCCGCCTGCT 
GCGGCCGAGC TCTCAGACTT CGGCCGAGAA GAACCTGAGG ACGGAGGGCT GGCCCCTGGA 
GAGGCCTCCC AGGGCTCAGA CTCGGAGGGC GGCGCCCAAG GCCTGGCCTC AGCTGGCAGG 
GACCGCACAG ACCAGACTTC GGAGGCAGCG CCTGACACCG CGGTGCCACC CAGTGTCAAA 
CCAAAGAAGC GGAGAAAGAG CTACCGGAGC TTCTTGCCTG AGAAGAGTGG CTACCCTGAC 
ATCGGCTTCC CCCTCTTCCC GCTTTCCAAG GGTTTCATCA AGAGCATCCG GGGGGTCCTG 
ACGCAGTTCC GGGCCGCCCT GATAGAGGCG GGCATGCCCC AGGGCGCCGA GGACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020922247.1
CDS	558..3977
Translation	MRSTDSQCGTVMDVSIDCAVKLIGTNCIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTPCPRDHSVEDAAGKKGGAKAKSETGSTSPEGTPGGSASPEEMQDEGPEEAQEAGEQLPPFLLKEAGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGASRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPLPATEPPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDAERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHALLERAQALPNGLPKDSSSPEPPAAAELSDFGREEPEDGGLAPGEASQGSDSEGGAQGLASAGRDRTDQTSEAAPDTAVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALIEAGMPQGAEDK

Target ORF information:

RefSeq Version	XM_021066588.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021066588.1

ORF Insert Sequence:

ATGAGATCCA CTGACAGCCA GTGCGGCACT GTGATGGACG TCAGCATCGA CTGCGCCGTC 
AAGCTCATCG GCACCAACTG CATCATCTAC CCCGTCAACA GCCGGGACCT CCAGCACATC 
TGGCCCTTCA TGTATGGGGA CTACATCGCC TACGACTGCT GGCTGGGGAA GGTCTACGAC 
CTGAAGAACC AGATCATCCT CAAGCTGTCC AACGGCGCCA GGTGCTCCAT GAACACAGAA 
GACGGCGCTA AGCTCTACGA CGTCTGCCCG CATGTCAGCG ACTCTGGCCT CTTCTTCGAT 
GACTCCTACG GCTTCTACCC AGGCCAGGTG CTCATCGGCC CTGCCAAGAT CTTCTCCAGC 
GTCCAGTGGC TCTCGGGGGT CAAGCCGGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG 
GTGGAAGAGG TGCAGGTGGT GGAGCTGAAG GTGACGTGGA TTACCAAGAG CTTCTGCCCA 
GGGGGCACAG ACAGCGTCAG CCCCCCGCCC TCTGTCATCA CCCAGGAAAA CCTAGGCAGG 
GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TGGGGGAGCG CTGTCTTTAT 
GTCTTCCCAG CCAAGGTAGA GCCGGCCAGG ATTGCCTGTG AATGTCCAGA AAAACACTGC 
GCCCAGGGGG AGGGCTCTAT GGCCAAGAAG GTGAAGCGCC TCTTGAAGAA GCAGGTCGTG 
AGGATCATGT CGTGCTCCCC GGACACCCCG TGCCCCCGGG ACCATTCCGT GGAGGACGCG 
GCGGGCAAGA AGGGGGGCGC CAAAGCCAAG AGCGAGACGG GGTCCACCAG CCCCGAGGGC 
ACGCCCGGCG GGTCCGCCAG CCCCGAGGAG ATGCAGGACG AGGGCCCGGA GGAGGCACAG 
GAGGCCGGCG AGCAGCTGCC CCCCTTCCTG CTGAAGGAAG CGGGGGACGA CAGGCTGCAC 
TCAGCCGAGC AGGACGCGGA TGATGAGGCC GCCGACGACA CGGATGACAC CAGCTCCGTG 
ACCTCCTCCG CCAGCTCCAC CACCTCCTCC CAGAGTGGCA GTGGCGCAAG TCGCAAAAAG 
AGCATACCCT TGTCTATCAA GAACCTAAAG CGGAAGCACA AGCGGAAGAA GAATAAAATC 
ACTCGCGACT TCAAGCCAGG GGACAGGGTG GCGGTGGAGG TGGTGACCAC AATGACCTCG 
GCGGACGTGA TGTGGCAGGA CGGCTCTGTG GAGTGCAGCA TCCGCTCCAA CGACCTCTTC 
CCCGTGCACC ACCTGGACAA CAACGAGTTC TGCCCCGGGG ACTTCGTGGT GGACAAGCGA 
GTCCAGAGCT GCCCGGATCC CGCTGTCTAT GGCGTGGTGC AGTCCGGGGA CCACGTGGGC 
CGCACCTGCA TGGTCAAGTG GTTCAAGCTG CGGCCGAGCG GAGACGACGT GGAGCTGATC 
GGGGAAGAGG AAGACGTGAG CGTCTACGAC ATCGCTGACC ACCCTGACTT TCGGTTCCGC 
ACAACCGACA TCGTCATCCG AATTGGCAAC ACGGAGGATG GAGCACCTCA CAAGGAGGAT 
GAGCCGTCGG TCGGCCAGGT GGCCCGCGTG GACGTCAGCA GCAAGGTGGA GGTGGTGTGG 
GCTGACAACT CCAAGACCAT CATCCTGCCG CAGCACCTGT ACAACATCGA GTCCGAGATC 
GAGGAGTCAG ACTATGACTC GGTCGAAGGC AGCACCAGCG GGGCATCCTC GGATGAGTGG 
GAGGACGACA GTGACAGCTG GGAGACGGAC AATGGGCTGG TGGAGGACGA GCACCCCAAG 
ATAGAGGAGC CCCCCCTCCC AGCCACCGAG CCGCCGGCAG CCCCCGAGGA GGACAAGGGT 
GTGGTGATCA GCGAGGAGGC CGCCACGGCC GCCATCCAGG GGGCCGTGGC CATGGCCGCC 
CCTGTGGCCG GGCTGATGGA GAAGGCTGGC AAGGACGGGC CCCCGAAGAG CTTCCGCGAG 
CTGAAGGAGG CCATCAAGAT CCTGGAGAGC CTCAAGAACA TGACCGTGGA GCAGCTCCTG 
ACGGGCTCGC CCACCTCCCC CACAGTGGAG CCCGAGAAGC CCACGCGGGA GAAGAAGTTT 
CTGGATGACA TCAAGAAGCT GCAGGAGAAC CTCAAGAAGA CCCTGGACAA CGTGGCCATC 
GCGGAGGAGG AGAAGATGGA GGCGGCGCCG GACGCGGAGC GCAAGGAGGA GAAGCCCGAG 
GGGCAGTCGC CCGTGAAGGC CGAGTGGCCC AGCGAGACGC CCGTGCTCTG CCAGCAGTGC 
GGCGGCAAGC CCGGGGTCAC CTTCACCAGC GCCAAGGGCG AGGTCTTCTC GGTGCTGGAG 
TTCGCGCCCT CAAATCACTC TTTTAAGAAG ATTGAGTTCC AGCCTCCAGA AGCCAAGAAG 
TTCTTCAGCA CAGTGCGGAA GGAGATGGCG CTGCTGGCCA CCTCGCTGCC CGATGGCATC 
ATGGTCAAGA CCTTTGAGGA CAGGATGGAC CTCTTCTCGG CCCTGATCAA AGGCCCCACT 
CGCACCCCGT ACGAGGACGG CCTCTACCTG TTTGACATCC AGCTCCCCAA CATCTACCCG 
GCCGTGCCCC CCCACTTCTG CTACCTCTCC CAGTGCAGCG GCCGCCTGAA CCCCAACCTG 
TATGACAACG GGAAGGTGTG CGTCAGCCTC CTGGGCACCT GGATTGGAAA GGGGACAGAG 
AGGTGGACGA GCAAATCCAG CCTTCTCCAG GTGCTCATCT CCATCCAAGG TCTCATCCTG 
GTGAATGAGC CCTATTACAA CGAAGCTGGC TTCGACAGCG ACCGCGGCCT GCAGGAGGGC 
TACGAAAACA GTCGCTGCTA CAACGAGATG GCGCTCATCC GAGTCGTGCA GTCCATGACC 
CAGCTGGTGC GGCGGCCGCC CGAGGTCTTC GAGCAGGAGA TCCGGCAGCA CTTCAGTACG 
GGCGGCTGGC GGCTGGTGAG CCGCATCGAG TCCTGGCTGG AGACCCACGC CCTGCTGGAG 
AGGGCCCAGG CGCTGCCCAA CGGGCTCCCC AAGGACAGCA GCTCGCCCGA GCCGCCTGCT 
GCGGCCGAGC TCTCAGACTT CGGCCGAGAA GAACCTGAGG ACGGAGGGCT GGCCCCTGGA 
GAGGCCTCCC AGGGCTCAGA CTCGGAGGGC GGCGCCCAAG GCCTGGCCTC AGCTGGCAGG 
GACCGCACAG ACCAGACTTC GGAGGCAGCG CCTGACACCG CGGTGCCACC CAGTGTCAAA 
CCAAAGAAGC GGAGAAAGAG CTACCGGAGC TTCTTGCCTG AGAAGAGTGG CTACCCTGAC 
ATCGGCTTCC CCCTCTTCCC GCTTTCCAAG GGTTTCATCA AGAGCATCCG GGGGGTCCTG 
ACGCAGTTCC GGGCCGCCCT GATAGAGGCG GGCATGCCCC AGGGCGCCGA GGACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_005656913.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005656970.1 Sus scrofa ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

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CloneID	OSe27760
Clone ID Related Accession (Same CDS sequence)	XM_005656913.2 , XM_005656913.3
Accession Version	XM_005656913.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005656913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCG GTCAGCAGCT GATTCTCAGC ATTCCGGCGT GTGGACCGCA GGAGCCCCGG 
ATGTTCTGGG TGGAGCTGAA GCTGGAGGAC CGCTCCGTGG TGCCCCGCGA CGTGGTCCGG 
CACATGAGAT CCACTGACAG CCAGTGCGGC ACTGTGATGG ACGTCAGCAT CGACTGCGCC 
GTCAAGCTCA TCGGCACCAA CTGCATCATC TACCCCGTCA ACAGCCGGGA CCTCCAGCAC 
ATCTGGCCCT TCATGTATGG GGACTACATC GCCTACGACT GCTGGCTGGG GAAGGTCTAC 
GACCTGAAGA ACCAGATCAT CCTCAAGCTG TCCAACGGCG CCAGGTGCTC CATGAACACA 
GAAGACGGCG CTAAGCTCTA CGACGTCTGC CCGCATGTCA GCGACTCTGG CCTCTTCTTC 
GATGACTCCT ACGGCTTCTA CCCAGGCCAG GTGCTCATCG GCCCTGCCAA GATCTTCTCC 
AGCGTCCAGT GGCTCTCGGG GGTCAAGCCG GTGCTCAGCA CCAAGAGCAA GTTCCGCGTG 
GTGGTGGAAG AGGTGCAGGT GGTGGAGCTG AAGGTGACGT GGATTACCAA GAGCTTCTGC 
CCAGGGGGCA CAGACAGCGT CAGCCCCCCG CCCTCTGTCA TCACCCAGGA AAACCTAGGC 
AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTGGGGGA GCGCTGTCTT 
TATGTCTTCC CAGCCAAGGT AGAGCCGGCC AGGATTGCCT GTGAATGTCC AGAAAAACAC 
TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTCTTGAA GAAGCAGGTC 
GTGAGGATCA TGTCGTGCTC CCCGGACACC CCGTGCCCCC GGGACCATTC CGTGGAGGAC 
GCGGCGGGCA AGAAGGGGGG CGCCAAAGCC AAGAGCGAGA CGGGGTCCAC CAGCCCCGAG 
GGCACGCCCG GCGGGTCCGC CAGCCCCGAG GAGATGCAGG ACGAGGGCCC GGAGGAGGCA 
CAGGAGGCCG GCGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGCGGGGGA CGACAGGCTG 
CACTCAGCCG AGCAGGACGC GGATGATGAG GCCGCCGACG ACACGGATGA CACCAGCTCC 
GTGACCTCCT CCGCCAGCTC CACCACCTCC TCCCAGAGTG GCAGTGGCGC AAGTCGCAAA 
AAGAGCATAC CCTTGTCTAT CAAGAACCTA AAGCGGAAGC ACAAGCGGAA GAAGAATAAA 
ATCACTCGCG ACTTCAAGCC AGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACAATGACC 
TCGGCGGACG TGATGTGGCA GGACGGCTCT GTGGAGTGCA GCATCCGCTC CAACGACCTC 
TTCCCCGTGC ACCACCTGGA CAACAACGAG TTCTGCCCCG GGGACTTCGT GGTGGACAAG 
CGAGTCCAGA GCTGCCCGGA TCCCGCTGTC TATGGCGTGG TGCAGTCCGG GGACCACGTG 
GGCCGCACCT GCATGGTCAA GTGGTTCAAG CTGCGGCCGA GCGGAGACGA CGTGGAGCTG 
ATCGGGGAAG AGGAAGACGT GAGCGTCTAC GACATCGCTG ACCACCCTGA CTTTCGGTTC 
CGCACAACCG ACATCGTCAT CCGAATTGGC AACACGGAGG ATGGAGCACC TCACAAGGAG 
GATGAGCCGT CGGTCGGCCA GGTGGCCCGC GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCCAAGAC CATCATCCTG CCGCAGCACC TGTACAACAT CGAGTCCGAG 
ATCGAGGAGT CAGACTATGA CTCGGTCGAA GGCAGCACCA GCGGGGCATC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAATGGGC TGGTGGAGGA CGAGCACCCC 
AAGATAGAGG AGCCCCCCCT CCCAGCCACC GAGCCGCCGG CAGCCCCCGA GGAGGACAAG 
GGTGTGGTGA TCAGCGAGGA GGCCGCCACG GCCGCCATCC AGGGGGCCGT GGCCATGGCC 
GCCCCTGTGG CCGGGCTGAT GGAGAAGGCT GGCAAGGACG GGCCCCCGAA GAGCTTCCGC 
GAGCTGAAGG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACCGT GGAGCAGCTC 
CTGACGGGCT CGCCCACCTC CCCCACAGTG GAGCCCGAGA AGCCCACGCG GGAGAAGAAG 
TTTCTGGATG ACATCAAGAA GCTGCAGGAG AACCTCAAGA AGACCCTGGA CAACGTGGCC 
ATCGCGGAGG AGGAGAAGAT GGAGGCGGCG CCGGACGCGG AGCGCAAGGA GGAGAAGCCC 
GAGGGGCAGT CGCCCGTGAA GGCCGAGTGG CCCAGCGAGA CGCCCGTGCT CTGCCAGCAG 
TGCGGCGGCA AGCCCGGGGT CACCTTCACC AGCGCCAAGG GCGAGGTCTT CTCGGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAGATTGAGT TCCAGCCTCC AGAAGCCAAG 
AAGTTCTTCA GCACAGTGCG GAAGGAGATG GCGCTGCTGG CCACCTCGCT GCCCGATGGC 
ATCATGGTCA AGACCTTTGA GGACAGGATG GACCTCTTCT CGGCCCTGAT CAAAGGCCCC 
ACTCGCACCC CGTACGAGGA CGGCCTCTAC CTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCGGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GCGGCCGCCT GAACCCCAAC 
CTGTATGACA ACGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATTGG AAAGGGGACA 
GAGAGGTGGA CGAGCAAATC CAGCCTTCTC CAGGTGCTCA TCTCCATCCA AGGTCTCATC 
CTGGTGAATG AGCCCTATTA CAACGAAGCT GGCTTCGACA GCGACCGCGG CCTGCAGGAG 
GGCTACGAAA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGAGTCGT GCAGTCCATG 
ACCCAGCTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA GCACTTCAGT 
ACGGGCGGCT GGCGGCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGCTC CCCAAGGACA GCAGCTCGCC CGAGCCGCCT 
GCTGCGGCCG AGCTCTCAGA CTTCGGCCGA GAAGAACCTG AGGACGGAGG GCTGGCCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AAGGCCTGGC CTCAGCTGGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCTGACA CCGCGGTGCC ACCCAGTGTC 
AAACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CTGAGAAGAG TGGCTACCCT 
GACATCGGCT TCCCCCTCTT CCCGCTTTCC AAGGGTTTCA TCAAGAGCAT CCGGGGGGTC 
CTGACGCAGT TCCGGGCCGC CCTGATAGAG GCGGGCATGC CCCAGGGCGC CGAGGACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005656970.1
CDS	19..3561
Translation	MAVGQQLILSIPACGPQEPRMFWVELKLEDRSVVPRDVVRHMRSTDSQCGTVMDVSIDCAVKLIGTNCIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTPCPRDHSVEDAAGKKGGAKAKSETGSTSPEGTPGGSASPEEMQDEGPEEAQEAGEQLPPFLLKEAGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGASRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPLPATEPPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDAERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHALLERAQALPNGLPKDSSSPEPPAAAELSDFGREEPEDGGLAPGEASQGSDSEGGAQGLASAGRDRTDQTSEAAPDTAVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALIEAGMPQGAEDK

Target ORF information:

RefSeq Version	XM_005656913.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005656913.2

ORF Insert Sequence:

ATGGCTGTCG GTCAGCAGCT GATTCTCAGC ATTCCGGCGT GTGGACCGCA GGAGCCCCGG 
ATGTTCTGGG TGGAGCTGAA GCTGGAGGAC CGCTCCGTGG TGCCCCGCGA CGTGGTCCGG 
CACATGAGAT CCACTGACAG CCAGTGCGGC ACTGTGATGG ACGTCAGCAT CGACTGCGCC 
GTCAAGCTCA TCGGCACCAA CTGCATCATC TACCCCGTCA ACAGCCGGGA CCTCCAGCAC 
ATCTGGCCCT TCATGTATGG GGACTACATC GCCTACGACT GCTGGCTGGG GAAGGTCTAC 
GACCTGAAGA ACCAGATCAT CCTCAAGCTG TCCAACGGCG CCAGGTGCTC CATGAACACA 
GAAGACGGCG CTAAGCTCTA CGACGTCTGC CCGCATGTCA GCGACTCTGG CCTCTTCTTC 
GATGACTCCT ACGGCTTCTA CCCAGGCCAG GTGCTCATCG GCCCTGCCAA GATCTTCTCC 
AGCGTCCAGT GGCTCTCGGG GGTCAAGCCG GTGCTCAGCA CCAAGAGCAA GTTCCGCGTG 
GTGGTGGAAG AGGTGCAGGT GGTGGAGCTG AAGGTGACGT GGATTACCAA GAGCTTCTGC 
CCAGGGGGCA CAGACAGCGT CAGCCCCCCG CCCTCTGTCA TCACCCAGGA AAACCTAGGC 
AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTGGGGGA GCGCTGTCTT 
TATGTCTTCC CAGCCAAGGT AGAGCCGGCC AGGATTGCCT GTGAATGTCC AGAAAAACAC 
TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTCTTGAA GAAGCAGGTC 
GTGAGGATCA TGTCGTGCTC CCCGGACACC CCGTGCCCCC GGGACCATTC CGTGGAGGAC 
GCGGCGGGCA AGAAGGGGGG CGCCAAAGCC AAGAGCGAGA CGGGGTCCAC CAGCCCCGAG 
GGCACGCCCG GCGGGTCCGC CAGCCCCGAG GAGATGCAGG ACGAGGGCCC GGAGGAGGCA 
CAGGAGGCCG GCGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGCGGGGGA CGACAGGCTG 
CACTCAGCCG AGCAGGACGC GGATGATGAG GCCGCCGACG ACACGGATGA CACCAGCTCC 
GTGACCTCCT CCGCCAGCTC CACCACCTCC TCCCAGAGTG GCAGTGGCGC AAGTCGCAAA 
AAGAGCATAC CCTTGTCTAT CAAGAACCTA AAGCGGAAGC ACAAGCGGAA GAAGAATAAA 
ATCACTCGCG ACTTCAAGCC AGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACAATGACC 
TCGGCGGACG TGATGTGGCA GGACGGCTCT GTGGAGTGCA GCATCCGCTC CAACGACCTC 
TTCCCCGTGC ACCACCTGGA CAACAACGAG TTCTGCCCCG GGGACTTCGT GGTGGACAAG 
CGAGTCCAGA GCTGCCCGGA TCCCGCTGTC TATGGCGTGG TGCAGTCCGG GGACCACGTG 
GGCCGCACCT GCATGGTCAA GTGGTTCAAG CTGCGGCCGA GCGGAGACGA CGTGGAGCTG 
ATCGGGGAAG AGGAAGACGT GAGCGTCTAC GACATCGCTG ACCACCCTGA CTTTCGGTTC 
CGCACAACCG ACATCGTCAT CCGAATTGGC AACACGGAGG ATGGAGCACC TCACAAGGAG 
GATGAGCCGT CGGTCGGCCA GGTGGCCCGC GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCCAAGAC CATCATCCTG CCGCAGCACC TGTACAACAT CGAGTCCGAG 
ATCGAGGAGT CAGACTATGA CTCGGTCGAA GGCAGCACCA GCGGGGCATC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAATGGGC TGGTGGAGGA CGAGCACCCC 
AAGATAGAGG AGCCCCCCCT CCCAGCCACC GAGCCGCCGG CAGCCCCCGA GGAGGACAAG 
GGTGTGGTGA TCAGCGAGGA GGCCGCCACG GCCGCCATCC AGGGGGCCGT GGCCATGGCC 
GCCCCTGTGG CCGGGCTGAT GGAGAAGGCT GGCAAGGACG GGCCCCCGAA GAGCTTCCGC 
GAGCTGAAGG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACCGT GGAGCAGCTC 
CTGACGGGCT CGCCCACCTC CCCCACAGTG GAGCCCGAGA AGCCCACGCG GGAGAAGAAG 
TTTCTGGATG ACATCAAGAA GCTGCAGGAG AACCTCAAGA AGACCCTGGA CAACGTGGCC 
ATCGCGGAGG AGGAGAAGAT GGAGGCGGCG CCGGACGCGG AGCGCAAGGA GGAGAAGCCC 
GAGGGGCAGT CGCCCGTGAA GGCCGAGTGG CCCAGCGAGA CGCCCGTGCT CTGCCAGCAG 
TGCGGCGGCA AGCCCGGGGT CACCTTCACC AGCGCCAAGG GCGAGGTCTT CTCGGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAGATTGAGT TCCAGCCTCC AGAAGCCAAG 
AAGTTCTTCA GCACAGTGCG GAAGGAGATG GCGCTGCTGG CCACCTCGCT GCCCGATGGC 
ATCATGGTCA AGACCTTTGA GGACAGGATG GACCTCTTCT CGGCCCTGAT CAAAGGCCCC 
ACTCGCACCC CGTACGAGGA CGGCCTCTAC CTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCGGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GCGGCCGCCT GAACCCCAAC 
CTGTATGACA ACGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATTGG AAAGGGGACA 
GAGAGGTGGA CGAGCAAATC CAGCCTTCTC CAGGTGCTCA TCTCCATCCA AGGTCTCATC 
CTGGTGAATG AGCCCTATTA CAACGAAGCT GGCTTCGACA GCGACCGCGG CCTGCAGGAG 
GGCTACGAAA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGAGTCGT GCAGTCCATG 
ACCCAGCTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA GCACTTCAGT 
ACGGGCGGCT GGCGGCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGCTC CCCAAGGACA GCAGCTCGCC CGAGCCGCCT 
GCTGCGGCCG AGCTCTCAGA CTTCGGCCGA GAAGAACCTG AGGACGGAGG GCTGGCCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AAGGCCTGGC CTCAGCTGGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCTGACA CCGCGGTGCC ACCCAGTGTC 
AAACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CTGAGAAGAG TGGCTACCCT 
GACATCGGCT TCCCCCTCTT CCCGCTTTCC AAGGGTTTCA TCAAGAGCAT CCGGGGGGTC 
CTGACGCAGT TCCGGGCCGC CCTGATAGAG GCGGGCATGC CCCAGGGCGC CGAGGACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_005656913.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005656970.1 Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe27760
Clone ID Related Accession (Same CDS sequence)	XM_005656913.2 , XM_005656913.3
Accession Version	XM_005656913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010454.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005656913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCG GTCAGCAGCT GATTCTCAGC ATTCCGGCGT GTGGACCGCA GGAGCCCCGG 
ATGTTCTGGG TGGAGCTGAA GCTGGAGGAC CGCTCCGTGG TGCCCCGCGA CGTGGTCCGG 
CACATGAGAT CCACTGACAG CCAGTGCGGC ACTGTGATGG ACGTCAGCAT CGACTGCGCC 
GTCAAGCTCA TCGGCACCAA CTGCATCATC TACCCCGTCA ACAGCCGGGA CCTCCAGCAC 
ATCTGGCCCT TCATGTATGG GGACTACATC GCCTACGACT GCTGGCTGGG GAAGGTCTAC 
GACCTGAAGA ACCAGATCAT CCTCAAGCTG TCCAACGGCG CCAGGTGCTC CATGAACACA 
GAAGACGGCG CTAAGCTCTA CGACGTCTGC CCGCATGTCA GCGACTCTGG CCTCTTCTTC 
GATGACTCCT ACGGCTTCTA CCCAGGCCAG GTGCTCATCG GCCCTGCCAA GATCTTCTCC 
AGCGTCCAGT GGCTCTCGGG GGTCAAGCCG GTGCTCAGCA CCAAGAGCAA GTTCCGCGTG 
GTGGTGGAAG AGGTGCAGGT GGTGGAGCTG AAGGTGACGT GGATTACCAA GAGCTTCTGC 
CCAGGGGGCA CAGACAGCGT CAGCCCCCCG CCCTCTGTCA TCACCCAGGA AAACCTAGGC 
AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTGGGGGA GCGCTGTCTT 
TATGTCTTCC CAGCCAAGGT AGAGCCGGCC AGGATTGCCT GTGAATGTCC AGAAAAACAC 
TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTCTTGAA GAAGCAGGTC 
GTGAGGATCA TGTCGTGCTC CCCGGACACC CCGTGCCCCC GGGACCATTC CGTGGAGGAC 
GCGGCGGGCA AGAAGGGGGG CGCCAAAGCC AAGAGCGAGA CGGGGTCCAC CAGCCCCGAG 
GGCACGCCCG GCGGGTCCGC CAGCCCCGAG GAGATGCAGG ACGAGGGCCC GGAGGAGGCA 
CAGGAGGCCG GCGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGCGGGGGA CGACAGGCTG 
CACTCAGCCG AGCAGGACGC GGATGATGAG GCCGCCGACG ACACGGATGA CACCAGCTCC 
GTGACCTCCT CCGCCAGCTC CACCACCTCC TCCCAGAGTG GCAGTGGCGC AAGTCGCAAA 
AAGAGCATAC CCTTGTCTAT CAAGAACCTA AAGCGGAAGC ACAAGCGGAA GAAGAATAAA 
ATCACTCGCG ACTTCAAGCC AGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACAATGACC 
TCGGCGGACG TGATGTGGCA GGACGGCTCT GTGGAGTGCA GCATCCGCTC CAACGACCTC 
TTCCCCGTGC ACCACCTGGA CAACAACGAG TTCTGCCCCG GGGACTTCGT GGTGGACAAG 
CGAGTCCAGA GCTGCCCGGA TCCCGCTGTC TATGGCGTGG TGCAGTCCGG GGACCACGTG 
GGCCGCACCT GCATGGTCAA GTGGTTCAAG CTGCGGCCGA GCGGAGACGA CGTGGAGCTG 
ATCGGGGAAG AGGAAGACGT GAGCGTCTAC GACATCGCTG ACCACCCTGA CTTTCGGTTC 
CGCACAACCG ACATCGTCAT CCGAATTGGC AACACGGAGG ATGGAGCACC TCACAAGGAG 
GATGAGCCGT CGGTCGGCCA GGTGGCCCGC GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCCAAGAC CATCATCCTG CCGCAGCACC TGTACAACAT CGAGTCCGAG 
ATCGAGGAGT CAGACTATGA CTCGGTCGAA GGCAGCACCA GCGGGGCATC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAATGGGC TGGTGGAGGA CGAGCACCCC 
AAGATAGAGG AGCCCCCCCT CCCAGCCACC GAGCCGCCGG CAGCCCCCGA GGAGGACAAG 
GGTGTGGTGA TCAGCGAGGA GGCCGCCACG GCCGCCATCC AGGGGGCCGT GGCCATGGCC 
GCCCCTGTGG CCGGGCTGAT GGAGAAGGCT GGCAAGGACG GGCCCCCGAA GAGCTTCCGC 
GAGCTGAAGG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACCGT GGAGCAGCTC 
CTGACGGGCT CGCCCACCTC CCCCACAGTG GAGCCCGAGA AGCCCACGCG GGAGAAGAAG 
TTTCTGGATG ACATCAAGAA GCTGCAGGAG AACCTCAAGA AGACCCTGGA CAACGTGGCC 
ATCGCGGAGG AGGAGAAGAT GGAGGCGGCG CCGGACGCGG AGCGCAAGGA GGAGAAGCCC 
GAGGGGCAGT CGCCCGTGAA GGCCGAGTGG CCCAGCGAGA CGCCCGTGCT CTGCCAGCAG 
TGCGGCGGCA AGCCCGGGGT CACCTTCACC AGCGCCAAGG GCGAGGTCTT CTCGGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAGATTGAGT TCCAGCCTCC AGAAGCCAAG 
AAGTTCTTCA GCACAGTGCG GAAGGAGATG GCGCTGCTGG CCACCTCGCT GCCCGATGGC 
ATCATGGTCA AGACCTTTGA GGACAGGATG GACCTCTTCT CGGCCCTGAT CAAAGGCCCC 
ACTCGCACCC CGTACGAGGA CGGCCTCTAC CTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCGGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GCGGCCGCCT GAACCCCAAC 
CTGTATGACA ACGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATTGG AAAGGGGACA 
GAGAGGTGGA CGAGCAAATC CAGCCTTCTC CAGGTGCTCA TCTCCATCCA AGGTCTCATC 
CTGGTGAATG AGCCCTATTA CAACGAAGCT GGCTTCGACA GCGACCGCGG CCTGCAGGAG 
GGCTACGAAA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGAGTCGT GCAGTCCATG 
ACCCAGCTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA GCACTTCAGT 
ACGGGCGGCT GGCGGCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGCTC CCCAAGGACA GCAGCTCGCC CGAGCCGCCT 
GCTGCGGCCG AGCTCTCAGA CTTCGGCCGA GAAGAACCTG AGGACGGAGG GCTGGCCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AAGGCCTGGC CTCAGCTGGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCTGACA CCGCGGTGCC ACCCAGTGTC 
AAACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CTGAGAAGAG TGGCTACCCT 
GACATCGGCT TCCCCCTCTT CCCGCTTTCC AAGGGTTTCA TCAAGAGCAT CCGGGGGGTC 
CTGACGCAGT TCCGGGCCGC CCTGATAGAG GCGGGCATGC CCCAGGGCGC CGAGGACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005656970.1
CDS	2245..5787
Translation	MAVGQQLILSIPACGPQEPRMFWVELKLEDRSVVPRDVVRHMRSTDSQCGTVMDVSIDCAVKLIGTNCIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTPCPRDHSVEDAAGKKGGAKAKSETGSTSPEGTPGGSASPEEMQDEGPEEAQEAGEQLPPFLLKEAGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGASRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPLPATEPPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDAERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHALLERAQALPNGLPKDSSSPEPPAAAELSDFGREEPEDGGLAPGEASQGSDSEGGAQGLASAGRDRTDQTSEAAPDTAVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALIEAGMPQGAEDK

Target ORF information:

RefSeq Version	XM_005656913.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005656913.3

ORF Insert Sequence:

ATGGCTGTCG GTCAGCAGCT GATTCTCAGC ATTCCGGCGT GTGGACCGCA GGAGCCCCGG 
ATGTTCTGGG TGGAGCTGAA GCTGGAGGAC CGCTCCGTGG TGCCCCGCGA CGTGGTCCGG 
CACATGAGAT CCACTGACAG CCAGTGCGGC ACTGTGATGG ACGTCAGCAT CGACTGCGCC 
GTCAAGCTCA TCGGCACCAA CTGCATCATC TACCCCGTCA ACAGCCGGGA CCTCCAGCAC 
ATCTGGCCCT TCATGTATGG GGACTACATC GCCTACGACT GCTGGCTGGG GAAGGTCTAC 
GACCTGAAGA ACCAGATCAT CCTCAAGCTG TCCAACGGCG CCAGGTGCTC CATGAACACA 
GAAGACGGCG CTAAGCTCTA CGACGTCTGC CCGCATGTCA GCGACTCTGG CCTCTTCTTC 
GATGACTCCT ACGGCTTCTA CCCAGGCCAG GTGCTCATCG GCCCTGCCAA GATCTTCTCC 
AGCGTCCAGT GGCTCTCGGG GGTCAAGCCG GTGCTCAGCA CCAAGAGCAA GTTCCGCGTG 
GTGGTGGAAG AGGTGCAGGT GGTGGAGCTG AAGGTGACGT GGATTACCAA GAGCTTCTGC 
CCAGGGGGCA CAGACAGCGT CAGCCCCCCG CCCTCTGTCA TCACCCAGGA AAACCTAGGC 
AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTGGGGGA GCGCTGTCTT 
TATGTCTTCC CAGCCAAGGT AGAGCCGGCC AGGATTGCCT GTGAATGTCC AGAAAAACAC 
TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTCTTGAA GAAGCAGGTC 
GTGAGGATCA TGTCGTGCTC CCCGGACACC CCGTGCCCCC GGGACCATTC CGTGGAGGAC 
GCGGCGGGCA AGAAGGGGGG CGCCAAAGCC AAGAGCGAGA CGGGGTCCAC CAGCCCCGAG 
GGCACGCCCG GCGGGTCCGC CAGCCCCGAG GAGATGCAGG ACGAGGGCCC GGAGGAGGCA 
CAGGAGGCCG GCGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGCGGGGGA CGACAGGCTG 
CACTCAGCCG AGCAGGACGC GGATGATGAG GCCGCCGACG ACACGGATGA CACCAGCTCC 
GTGACCTCCT CCGCCAGCTC CACCACCTCC TCCCAGAGTG GCAGTGGCGC AAGTCGCAAA 
AAGAGCATAC CCTTGTCTAT CAAGAACCTA AAGCGGAAGC ACAAGCGGAA GAAGAATAAA 
ATCACTCGCG ACTTCAAGCC AGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACAATGACC 
TCGGCGGACG TGATGTGGCA GGACGGCTCT GTGGAGTGCA GCATCCGCTC CAACGACCTC 
TTCCCCGTGC ACCACCTGGA CAACAACGAG TTCTGCCCCG GGGACTTCGT GGTGGACAAG 
CGAGTCCAGA GCTGCCCGGA TCCCGCTGTC TATGGCGTGG TGCAGTCCGG GGACCACGTG 
GGCCGCACCT GCATGGTCAA GTGGTTCAAG CTGCGGCCGA GCGGAGACGA CGTGGAGCTG 
ATCGGGGAAG AGGAAGACGT GAGCGTCTAC GACATCGCTG ACCACCCTGA CTTTCGGTTC 
CGCACAACCG ACATCGTCAT CCGAATTGGC AACACGGAGG ATGGAGCACC TCACAAGGAG 
GATGAGCCGT CGGTCGGCCA GGTGGCCCGC GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCCAAGAC CATCATCCTG CCGCAGCACC TGTACAACAT CGAGTCCGAG 
ATCGAGGAGT CAGACTATGA CTCGGTCGAA GGCAGCACCA GCGGGGCATC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAATGGGC TGGTGGAGGA CGAGCACCCC 
AAGATAGAGG AGCCCCCCCT CCCAGCCACC GAGCCGCCGG CAGCCCCCGA GGAGGACAAG 
GGTGTGGTGA TCAGCGAGGA GGCCGCCACG GCCGCCATCC AGGGGGCCGT GGCCATGGCC 
GCCCCTGTGG CCGGGCTGAT GGAGAAGGCT GGCAAGGACG GGCCCCCGAA GAGCTTCCGC 
GAGCTGAAGG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACCGT GGAGCAGCTC 
CTGACGGGCT CGCCCACCTC CCCCACAGTG GAGCCCGAGA AGCCCACGCG GGAGAAGAAG 
TTTCTGGATG ACATCAAGAA GCTGCAGGAG AACCTCAAGA AGACCCTGGA CAACGTGGCC 
ATCGCGGAGG AGGAGAAGAT GGAGGCGGCG CCGGACGCGG AGCGCAAGGA GGAGAAGCCC 
GAGGGGCAGT CGCCCGTGAA GGCCGAGTGG CCCAGCGAGA CGCCCGTGCT CTGCCAGCAG 
TGCGGCGGCA AGCCCGGGGT CACCTTCACC AGCGCCAAGG GCGAGGTCTT CTCGGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAGATTGAGT TCCAGCCTCC AGAAGCCAAG 
AAGTTCTTCA GCACAGTGCG GAAGGAGATG GCGCTGCTGG CCACCTCGCT GCCCGATGGC 
ATCATGGTCA AGACCTTTGA GGACAGGATG GACCTCTTCT CGGCCCTGAT CAAAGGCCCC 
ACTCGCACCC CGTACGAGGA CGGCCTCTAC CTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCGGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GCGGCCGCCT GAACCCCAAC 
CTGTATGACA ACGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATTGG AAAGGGGACA 
GAGAGGTGGA CGAGCAAATC CAGCCTTCTC CAGGTGCTCA TCTCCATCCA AGGTCTCATC 
CTGGTGAATG AGCCCTATTA CAACGAAGCT GGCTTCGACA GCGACCGCGG CCTGCAGGAG 
GGCTACGAAA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGAGTCGT GCAGTCCATG 
ACCCAGCTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA GCACTTCAGT 
ACGGGCGGCT GGCGGCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGCTC CCCAAGGACA GCAGCTCGCC CGAGCCGCCT 
GCTGCGGCCG AGCTCTCAGA CTTCGGCCGA GAAGAACCTG AGGACGGAGG GCTGGCCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AAGGCCTGGC CTCAGCTGGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCTGACA CCGCGGTGCC ACCCAGTGTC 
AAACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CTGAGAAGAG TGGCTACCCT 
GACATCGGCT TCCCCCTCTT CCCGCTTTCC AAGGGTTTCA TCAAGAGCAT CCGGGGGGTC 
CTGACGCAGT TCCGGGCCGC CCTGATAGAG GCGGGCATGC CCCAGGGCGC CGAGGACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_013989884.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013845338.1 Sus scrofa ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

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CloneID	OSe40881
Clone ID Related Accession (Same CDS sequence)	XM_013989884.1
Accession Version	XM_013989884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGCT TTCAGCTGAA GCTGGAGGAC CGCTCCGTGG TGCCCCGCGA CGTGGTCCGG 
CACATGAGAT CCACTGACAG CCAGTGCGGC ACTGTGATGG ACGTCAGCAT CGACTGCGCC 
GTCAAGCTCA TCGGCACCAA CTGCATCATC TACCCCGTCA ACAGCCGGGA CCTCCAGCAC 
ATCTGGCCCT TCATGTATGG GGACTACATC GCCTACGACT GCTGGCTGGG GAAGGTCTAC 
GACCTGAAGA ACCAGATCAT CCTCAAGCTG TCCAACGGCG CCAGGTGCTC CATGAACACA 
GAAGACGGCG CTAAGCTCTA CGACGTCTGC CCGCATGTCA GCGACTCTGG CCTCTTCTTC 
GATGACTCCT ACGGCTTCTA CCCAGGCCAG GTGCTCATCG GCCCTGCCAA GATCTTCTCC 
AGCGTCCAGT GGCTCTCGGG GGTCAAGCCG GTGCTCAGCA CCAAGAGCAA GTTCCGCGTG 
GTGGTGGAAG AGGTGCAGGT GGTGGAGCTG AAGGTGACGT GGATTACCAA GAGCTTCTGC 
CCAGGGGGCA CAGACAGCGT CAGCCCCCCG CCCTCTGTCA TCACCCAGGA AAACCTAGGC 
AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTGGGGGA GCGCTGTCTT 
TATGTCTTCC CAGCCAAGGT AGAGCCGGCC AGGATTGCCT GTGAATGTCC AGAAAAACAC 
TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTCTTGAA GAAGCAGGTC 
GTGAGGATCA TGTCGTGCTC CCCGGACACC CCGTGCCCCC GGGACCATTC CGTGGAGGAC 
GCGGCGGGCA AGAAGGGGGG CGCCAAAGCC AAGAGCGAGA CGGGGTCCAC CAGCCCCGAG 
GGCACGCCCG GCGGGTCCGC CAGCCCCGAG GAGATGCAGG ACGAGGGCCC GGAGGAGGCA 
CAGGAGGCCG GCGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGCGGGGGA CGACAGGCTG 
CACTCAGCCG AGCAGGACGC GGATGATGAG GCCGCCGACG ACACGGATGA CACCAGCTCC 
GTGACCTCCT CCGCCAGCTC CACCACCTCC TCCCAGAGTG GCAGTGGCGC AAGTCGCAAA 
AAGAGCATAC CCTTGTCTAT CAAGAACCTA AAGCGGAAGC ACAAGCGGAA GAAGAATAAA 
ATCACTCGCG ACTTCAAGCC AGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACAATGACC 
TCGGCGGACG TGATGTGGCA GGACGGCTCT GTGGAGTGCA GCATCCGCTC CAACGACCTC 
TTCCCCGTGC ACCACCTGGA CAACAACGAG TTCTGCCCCG GGGACTTCGT GGTGGACAAG 
CGAGTCCAGA GCTGCCCGGA TCCCGCTGTC TATGGCGTGG TGCAGTCCGG GGACCACGTG 
GGCCGCACCT GCATGGTCAA GTGGTTCAAG CTGCGGCCGA GCGGAGACGA CGTGGAGCTG 
ATCGGGGAAG AGGAAGACGT GAGCGTCTAC GACATCGCTG ACCACCCTGA CTTTCGGTTC 
CGCACAACCG ACATCGTCAT CCGAATTGGC AACACGGAGG ATGGAGCACC TCACAAGGAG 
GATGAGCCGT CGGTCGGCCA GGTGGCCCGC GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCCAAGAC CATCATCCTG CCGCAGCACC TGTACAACAT CGAGTCCGAG 
ATCGAGGAGT CAGACTATGA CTCGGTCGAA GGCAGCACCA GCGGGGCATC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAATGGGC TGGTGGAGGA CGAGCACCCC 
AAGATAGAGG AGCCCCCCCT CCCAGCCACC GAGCCGCCGG CAGCCCCCGA GGAGGACAAG 
GGTGTGGTGA TCAGCGAGGA GGCCGCCACG GCCGCCATCC AGGGGGCCGT GGCCATGGCC 
GCCCCTGTGG CCGGGCTGAT GGAGAAGGCT GGCAAGGACG GGCCCCCGAA GAGCTTCCGC 
GAGCTGAAGG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACCGT GGAGCAGCTC 
CTGACGGGCT CGCCCACCTC CCCCACAGTG GAGCCCGAGA AGCCCACGCG GGAGAAGAAG 
TTTCTGGATG ACATCAAGAA GCTGCAGGAG AACCTCAAGA AGACCCTGGA CAACGTGGCC 
ATCGCGGAGG AGGAGAAGAT GGAGGCGGCG CCGGACGCGG AGCGCAAGGA GGAGAAGCCC 
GAGGGGCAGT CGCCCGTGAA GGCCGAGTGG CCCAGCGAGA CGCCCGTGCT CTGCCAGCAG 
TGCGGCGGCA AGCCCGGGGT CACCTTCACC AGCGCCAAGG GCGAGGTCTT CTCGGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAGATTGAGT TCCAGCCTCC AGAAGCCAAG 
AAGTTCTTCA GCACAGTGCG GAAGGAGATG GCGCTGCTGG CCACCTCGCT GCCCGATGGC 
ATCATGGTCA AGACCTTTGA GGACAGGATG GACCTCTTCT CGGCCCTGAT CAAAGGCCCC 
ACTCGCACCC CGTACGAGGA CGGCCTCTAC CTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCGGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GCGGCCGCCT GAACCCCAAC 
CTGTATGACA ACGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATTGG AAAGGGGACA 
GAGAGGTGGA CGAGCAAATC CAGCCTTCTC CAGGTGCTCA TCTCCATCCA AGGTCTCATC 
CTGGTGAATG AGCCCTATTA CAACGAAGCT GGCTTCGACA GCGACCGCGG CCTGCAGGAG 
GGCTACGAAA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGAGTCGT GCAGTCCATG 
ACCCAGCTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA GCACTTCAGT 
ACGGGCGGCT GGCGGCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGCTC CCCAAGGACA GCAGCTCGCC CGAGCCGCCT 
GCTGCGGCCG AGCTCTCAGA CTTCGGCCGA GAAGAACCTG AGGACGGAGG GCTGGCCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AAGGCCTGGC CTCAGCTGGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCTGACA CCGCGGTGCC ACCCAGTGTC 
AAACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CTGAGAAGAG TGGCTACCCT 
GACATCGGCT TCCCCCTCTT CCCGCTTTCC AAGGGTTTCA TCAAGAGCAT CCGGGGGGTC 
CTGACGCAGT TCCGGGCCGC CCTGATAGAG GCGGGCATGC CCCAGGGCGC CGAGGACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013845338.1
CDS	16..3498
Translation	MTRFQLKLEDRSVVPRDVVRHMRSTDSQCGTVMDVSIDCAVKLIGTNCIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTPCPRDHSVEDAAGKKGGAKAKSETGSTSPEGTPGGSASPEEMQDEGPEEAQEAGEQLPPFLLKEAGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGASRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPLPATEPPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDAERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHALLERAQALPNGLPKDSSSPEPPAAAELSDFGREEPEDGGLAPGEASQGSDSEGGAQGLASAGRDRTDQTSEAAPDTAVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALIEAGMPQGAEDK

Target ORF information:

RefSeq Version	XM_013989884.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013989884.1

ORF Insert Sequence:

ATGACTCGCT TTCAGCTGAA GCTGGAGGAC CGCTCCGTGG TGCCCCGCGA CGTGGTCCGG 
CACATGAGAT CCACTGACAG CCAGTGCGGC ACTGTGATGG ACGTCAGCAT CGACTGCGCC 
GTCAAGCTCA TCGGCACCAA CTGCATCATC TACCCCGTCA ACAGCCGGGA CCTCCAGCAC 
ATCTGGCCCT TCATGTATGG GGACTACATC GCCTACGACT GCTGGCTGGG GAAGGTCTAC 
GACCTGAAGA ACCAGATCAT CCTCAAGCTG TCCAACGGCG CCAGGTGCTC CATGAACACA 
GAAGACGGCG CTAAGCTCTA CGACGTCTGC CCGCATGTCA GCGACTCTGG CCTCTTCTTC 
GATGACTCCT ACGGCTTCTA CCCAGGCCAG GTGCTCATCG GCCCTGCCAA GATCTTCTCC 
AGCGTCCAGT GGCTCTCGGG GGTCAAGCCG GTGCTCAGCA CCAAGAGCAA GTTCCGCGTG 
GTGGTGGAAG AGGTGCAGGT GGTGGAGCTG AAGGTGACGT GGATTACCAA GAGCTTCTGC 
CCAGGGGGCA CAGACAGCGT CAGCCCCCCG CCCTCTGTCA TCACCCAGGA AAACCTAGGC 
AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTGGGGGA GCGCTGTCTT 
TATGTCTTCC CAGCCAAGGT AGAGCCGGCC AGGATTGCCT GTGAATGTCC AGAAAAACAC 
TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTCTTGAA GAAGCAGGTC 
GTGAGGATCA TGTCGTGCTC CCCGGACACC CCGTGCCCCC GGGACCATTC CGTGGAGGAC 
GCGGCGGGCA AGAAGGGGGG CGCCAAAGCC AAGAGCGAGA CGGGGTCCAC CAGCCCCGAG 
GGCACGCCCG GCGGGTCCGC CAGCCCCGAG GAGATGCAGG ACGAGGGCCC GGAGGAGGCA 
CAGGAGGCCG GCGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGCGGGGGA CGACAGGCTG 
CACTCAGCCG AGCAGGACGC GGATGATGAG GCCGCCGACG ACACGGATGA CACCAGCTCC 
GTGACCTCCT CCGCCAGCTC CACCACCTCC TCCCAGAGTG GCAGTGGCGC AAGTCGCAAA 
AAGAGCATAC CCTTGTCTAT CAAGAACCTA AAGCGGAAGC ACAAGCGGAA GAAGAATAAA 
ATCACTCGCG ACTTCAAGCC AGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACAATGACC 
TCGGCGGACG TGATGTGGCA GGACGGCTCT GTGGAGTGCA GCATCCGCTC CAACGACCTC 
TTCCCCGTGC ACCACCTGGA CAACAACGAG TTCTGCCCCG GGGACTTCGT GGTGGACAAG 
CGAGTCCAGA GCTGCCCGGA TCCCGCTGTC TATGGCGTGG TGCAGTCCGG GGACCACGTG 
GGCCGCACCT GCATGGTCAA GTGGTTCAAG CTGCGGCCGA GCGGAGACGA CGTGGAGCTG 
ATCGGGGAAG AGGAAGACGT GAGCGTCTAC GACATCGCTG ACCACCCTGA CTTTCGGTTC 
CGCACAACCG ACATCGTCAT CCGAATTGGC AACACGGAGG ATGGAGCACC TCACAAGGAG 
GATGAGCCGT CGGTCGGCCA GGTGGCCCGC GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCCAAGAC CATCATCCTG CCGCAGCACC TGTACAACAT CGAGTCCGAG 
ATCGAGGAGT CAGACTATGA CTCGGTCGAA GGCAGCACCA GCGGGGCATC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAATGGGC TGGTGGAGGA CGAGCACCCC 
AAGATAGAGG AGCCCCCCCT CCCAGCCACC GAGCCGCCGG CAGCCCCCGA GGAGGACAAG 
GGTGTGGTGA TCAGCGAGGA GGCCGCCACG GCCGCCATCC AGGGGGCCGT GGCCATGGCC 
GCCCCTGTGG CCGGGCTGAT GGAGAAGGCT GGCAAGGACG GGCCCCCGAA GAGCTTCCGC 
GAGCTGAAGG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACCGT GGAGCAGCTC 
CTGACGGGCT CGCCCACCTC CCCCACAGTG GAGCCCGAGA AGCCCACGCG GGAGAAGAAG 
TTTCTGGATG ACATCAAGAA GCTGCAGGAG AACCTCAAGA AGACCCTGGA CAACGTGGCC 
ATCGCGGAGG AGGAGAAGAT GGAGGCGGCG CCGGACGCGG AGCGCAAGGA GGAGAAGCCC 
GAGGGGCAGT CGCCCGTGAA GGCCGAGTGG CCCAGCGAGA CGCCCGTGCT CTGCCAGCAG 
TGCGGCGGCA AGCCCGGGGT CACCTTCACC AGCGCCAAGG GCGAGGTCTT CTCGGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAGATTGAGT TCCAGCCTCC AGAAGCCAAG 
AAGTTCTTCA GCACAGTGCG GAAGGAGATG GCGCTGCTGG CCACCTCGCT GCCCGATGGC 
ATCATGGTCA AGACCTTTGA GGACAGGATG GACCTCTTCT CGGCCCTGAT CAAAGGCCCC 
ACTCGCACCC CGTACGAGGA CGGCCTCTAC CTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCGGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GCGGCCGCCT GAACCCCAAC 
CTGTATGACA ACGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATTGG AAAGGGGACA 
GAGAGGTGGA CGAGCAAATC CAGCCTTCTC CAGGTGCTCA TCTCCATCCA AGGTCTCATC 
CTGGTGAATG AGCCCTATTA CAACGAAGCT GGCTTCGACA GCGACCGCGG CCTGCAGGAG 
GGCTACGAAA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGAGTCGT GCAGTCCATG 
ACCCAGCTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA GCACTTCAGT 
ACGGGCGGCT GGCGGCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGCTC CCCAAGGACA GCAGCTCGCC CGAGCCGCCT 
GCTGCGGCCG AGCTCTCAGA CTTCGGCCGA GAAGAACCTG AGGACGGAGG GCTGGCCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AAGGCCTGGC CTCAGCTGGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCTGACA CCGCGGTGCC ACCCAGTGTC 
AAACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CTGAGAAGAG TGGCTACCCT 
GACATCGGCT TCCCCCTCTT CCCGCTTTCC AAGGGTTTCA TCAAGAGCAT CCGGGGGGTC 
CTGACGCAGT TCCGGGCCGC CCTGATAGAG GCGGGCATGC CCCAGGGCGC CGAGGACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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UBE2O cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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