SPTBN1 cDNA ORF clone, Sus scrofa(pig)

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe246959 XM_021088222.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe246959 XM_021088220.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe246959 XM_021088219.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe246959 XM_021088218.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe246959 XM_021088221.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe246960 XM_021088223.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe246961 XM_021088224.1
Latest version!
Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe246959
    Clone ID Related Accession (Same CDS sequence) XM_021088220.1 , XM_021088222.1 , XM_021088221.1 , XM_021088218.1 , XM_021088219.1
    Accession Version XM_021088220.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943879.1
    CDS80..7174
    Translation

    Target ORF information:

    RefSeq Version XM_021088220.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe246959
    Clone ID Related Accession (Same CDS sequence) XM_021088220.1 , XM_021088222.1 , XM_021088221.1 , XM_021088218.1 , XM_021088219.1
    Accession Version XM_021088222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943881.1
    CDS67..7161
    Translation

    Target ORF information:

    RefSeq Version XM_021088222.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe246959
    Clone ID Related Accession (Same CDS sequence) XM_021088220.1 , XM_021088222.1 , XM_021088221.1 , XM_021088218.1 , XM_021088219.1
    Accession Version XM_021088221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943880.1
    CDS68..7162
    Translation

    Target ORF information:

    RefSeq Version XM_021088221.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe246959
    Clone ID Related Accession (Same CDS sequence) XM_021088220.1 , XM_021088222.1 , XM_021088221.1 , XM_021088218.1 , XM_021088219.1
    Accession Version XM_021088218.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943877.1
    CDS283..7377
    Translation

    Target ORF information:

    RefSeq Version XM_021088218.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe246959
    Clone ID Related Accession (Same CDS sequence) XM_021088220.1 , XM_021088222.1 , XM_021088221.1 , XM_021088218.1 , XM_021088219.1
    Accession Version XM_021088219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943878.1
    CDS73..7167
    Translation

    Target ORF information:

    RefSeq Version XM_021088219.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC GAGTGAAATG GTCAACGGCG CCGCAGAACA GAGGACAAGC
    TCGAAGGAGT CCAGCCCCAT CCCCTCCCCA ACCTCCGACC GCAAAGCCAA GACCTCCCTC
    CCTGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCCTC GGCCCAGATG
    GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCCCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTACTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGACGCA
    AAGACAGCTG CTTCCGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTCGA TTACAAAAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT
    GATGGCAACG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC GTGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCTCA GACCTTGCCC ACCAGCGCCG TGACCATCAC CAGCGAGTCC
    AGTCCCGGCA AACGGGAGAA GGACAAAGAG AAAGACAAGG AAAAGAGGTT CAGCCTTTTT
    GGCAAGAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe246960
    Clone ID Related Accession (Same CDS sequence) XM_021088223.1
    Accession Version XM_021088223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTG TAGCGTCTCC GGGCCGCTAT CGCCGGCTTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAAC AGCTACAAGA TGAGCGCGAA
    GCCGTGCAAA AGAAGACCTT TACCAAGTGG GTCAACTCCC ACCTCGCCCG AGTGTCCTGC
    CGGATCACAG ACCTGTACAC CGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGACT GCCCAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT GGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGGT TCCAGATCCA GGACATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AACATTCACA ATTTCACTAC CAGCTGGAGG GACGGCATGG CCTTCAACGC CCTGATACAC
    AAGCACAGGC CTGACCTGAT AGATTTTGAC AAACTGAAGA AATCCAATGC ACACTACAAT
    CTGCAGAATG CGTTTAACTT GGCGGAGCAG CACCTTGGCC TCACCAAACT GTTGGACCCT
    GAAGATATCA GCGTGGACCA CCCCGATGAG AAGTCCATTA TCACTTACGT GGTGACTTAT
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAGCGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC TGAAAAAATG ATTGAAAAGT ATGAATCCCT TGCCTCTGAT
    CTTCTGGAGT GGATTGAACA AACCATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCG
    CTGGTTGGGG TCCAACAACA GCTTCAGGCC TTCAACACTT ACCGCACTGT GGAGAAACCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACTATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGATATTAAC
    AAGGCCTGGG AACGACTGGA AAAAGCAGAG CACGAGAGAG AACTGGCCCT GCGGAACGAG
    CTTATTCGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TCGAGGCCGC CACGAAAAAG CACGAGGCCA TTGAGACGGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTTGCC GTGGCCAGGG AGCTGGAAGC GGAGAACTAC
    CATGACATCA AGCGCATCAC GGCAAGGAAG GACAACGTCA TCCGGCTCTG GGAATACCTG
    CTGGAACTGC TCAGGGCCCG GAGGCAGCGC CTCGAGATGA ACCTGGGCCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT ATTACTGTCT
    CAGGACTATG GCAAACACTT GCTGGGCGTG GAAGACCTGT TGCAGAAGCA TGCCCTGGTT
    GAAGCGGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC TGCCCAGAAG
    TTTGCAACAG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGTTGG CAGCAGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGGCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAGGA GGAAGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GCAAGGACCT CACCAGTGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGACGAGA TGAGCGGCCG CAGCGGTCAC
    TTCGAGCAGG CCATCAAAGA GGGCGAGGAC ATGATCGCTG AGGAGCACTT TGGGTCGGAG
    AAGATCCGGG AGCGGATCGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCACTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ACGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCTCTGGTC AAGAAGCACA AGGATGTGGC CGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAGG CCGGTGCCCT CCCCCAGGAG
    CACGCCGAGT CCCCCGATGT ACGGGGCCGG CTCGCGGGCA TCGAGGAGCG CTACAAGGAG
    GTGGCTGAAC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGCGAGCTC TGGATCGATG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    TTAGAACCAG AGATGAACAA CCAAGCTTCC CGAGTGGCGG TGGTGAATCA GATTGCACGG
    CAGCTGATGC ACAGCGGCCA CCCAAGCGAG AAGGAGATCA AGGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGTCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC ACTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAGTCCTG GATCCGGGAA
    AAGACCAAGG TCATCGAATC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    TTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATCGAGGC GAAGCTGAGC
    GACCTGCAGA AGGAGGCTGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAAACCAC GCTGAGGAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCAG GACCCAGACG GCCATCGCCT CGGAGGACAT GCCCAACACC
    CTGACCGAAG CCGAGAAGCT CCTCACACAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGTGAGA TGGTCACGCA GGGCCAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAATGAG
    CTCCACAAGA TGTGGGAGAA CAGACAGAAC CTCCTTTCCC AGTCCCACGC CCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCTGAAGCC TTTCTCAACA ACCAGGAGTA TGTCTTGGCC
    CATACTGAGA TGCCCACCAC CTTGGAAGGG GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CAGTCGTGGA GACCGGCCGC
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AAGAGAAGGT GGACTCCATC
    GATGACAGAC ATAGGAAGAA TCGCGAAGCA GCAAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAAGAGT TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTC GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCGGAGA CGGAAGCCGT GGTAAAGGAG
    AAACTCACAG GTTTGCATAA AATGTGGGAA GTCCTTGAAT CCACCACGCA GACCAAGGCC
    CAGCGGCTCT TCGATGCAAA CAAGGCTGAA CTCTTCACCC AGAGCTGTGC AGACCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAATCTGATG ATTACGGCAA AGACCTCACC
    AGTGTCAACA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATTG AGGAGCTCCA GAGCCAGGCA CAGGCCCTGA GCCAGGAGGG GAAGAGCACA
    GACGAGGTGG ACAGCAAGCG GCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTAAATGAGA GGAAGCAGAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT TAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAATC TCCAGACCGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATTTTCGAGA GGAGCCAGAA CATCGTTGCC
    GACAGCAGCA GCCTCAGTGC CGAGGCCATC CGGCAGAGGC TGGCCGACCT GAAGCAGCTG
    TGGGGCCAGC TCATCGAGGA GACGGAGAAG CGGCACCGGC GGCTGGAGGA GGCACACAGG
    GCCCAGCAGT ACTACTTCGA TGCTGCCGAG GCCGAGGCGT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CAGTCTCTAT GTTGAAGAAG
    CACCAGATTC TGGAACAAGC AGTGGAGGAC TACGCAGAGA CGGTACATCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTAGC CGACAGCCAT CCTGAAAGCG AGCGCATCAG CATGCGGCAA
    TCCAAGGTGG ACAAGCTGTA CGCGGGCCTC AAGGACCTGG CCGAGGAGAG GAGGGGCAAG
    CTGGACGAGA GGCACCGGCT GTTCCAGCTC AACCGCGAGG TGGACGACCT GGAGCAGTGG
    ATTGCCGAGA GGGAGGTGGT CGCAGGGTCT CACGAGTTGG GGCAGGACTA CGAGCATGTC
    ACGATGCTAC AAGAACGGTT CCGGGAGTTT GCCCGAGACA CGGGAAACAT CGGGCAGGAG
    CGCGTGGACA CTGTCAATCA CATGGCAGAT GAGCTCATCA ACTCCGGACA TTCAGATGCT
    GCCACCATTG CCGAGTGGAA GGACGGGCTC AACGAAGCCT GGGCAGACCT CCTGGAGCTC
    ATTGACACGA GAACACAGAT CCTTGCCGCC TCCTACGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGACG CATCCAGGAC AAACACAAGA AACTCCCCGA GGAGCTCGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCATA CCACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGACG CAGCCCGCCT CCAGGCAGCC
    TATGCAGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ACGAGGTCCT AGAAGCCTGG
    AAGGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTATGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT CGATGCTCGA AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAGTCGC TGCTGGCGAG GAAGCACTAC GCGTCAGAGG AGATCAAGGA GAAGTTACTG
    CAGTTGACGG ACAAGAGAAA AGAAATGATT GACAAATGGG AAGACCGATG GGAGTGGTTA
    AGACTGATCT TGGAGGTCCA TCAGTTCTCA AGGGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAAATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCATGA GGCTTTTGAA AAGTCTGCAG CGACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AACGGTTGAC GACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCCAGCA CAAAGGTTTC CGAGGAGACC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA TGGTTTGCCG
    ACCGAGCAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CGAGTGAAAT GGTCAACGGC
    GCCGCAGAAC AGAGGACAAG CTCGAAGGAG TCCAGCCCCA TCCCCTCCCC AACCTCCGAC
    CGCAAAGCCA AGACCTCCCT CCCTGCCCAG AGTGCCGCCA CCTTGCCGGC CAGAACCCAG
    GAGACGCCCT CGGCCCAGAT GGAAGGCTTC CTGAATCGGA AGCACGAGTG GGAGGCCCAC
    AACAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTACT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGACGC AAAGACAGCT GCTTCCGGAA TTCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTCG ATTACAAAAA GAAGAAACAC
    GTGTTCAAGC TAAGACTAAA TGATGGCAAC GAGTACCTCT TCCAAGCCAA AGATGATGAG
    GAAATGAACA CGTGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCCGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCTC AGACCTTGCC CACCAGCGCC
    GTGACCATCA CCAGCGAGTC CAGTCCCGGC AAACGGGAGA AGGACAAAGA GAAAGACAAG
    GAAAAGAGGT TCAGCCTTTT TGGCAAGAAG AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943882.1
    CDS495..7550
    Translation

    Target ORF information:

    RefSeq Version XM_021088223.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTG TAGCGTCTCC GGGCCGCTAT CGCCGGCTTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAAC AGCTACAAGA TGAGCGCGAA
    GCCGTGCAAA AGAAGACCTT TACCAAGTGG GTCAACTCCC ACCTCGCCCG AGTGTCCTGC
    CGGATCACAG ACCTGTACAC CGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGACT GCCCAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT GGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGGT TCCAGATCCA GGACATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AACATTCACA ATTTCACTAC CAGCTGGAGG GACGGCATGG CCTTCAACGC CCTGATACAC
    AAGCACAGGC CTGACCTGAT AGATTTTGAC AAACTGAAGA AATCCAATGC ACACTACAAT
    CTGCAGAATG CGTTTAACTT GGCGGAGCAG CACCTTGGCC TCACCAAACT GTTGGACCCT
    GAAGATATCA GCGTGGACCA CCCCGATGAG AAGTCCATTA TCACTTACGT GGTGACTTAT
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAGCGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC TGAAAAAATG ATTGAAAAGT ATGAATCCCT TGCCTCTGAT
    CTTCTGGAGT GGATTGAACA AACCATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCG
    CTGGTTGGGG TCCAACAACA GCTTCAGGCC TTCAACACTT ACCGCACTGT GGAGAAACCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACTATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGATATTAAC
    AAGGCCTGGG AACGACTGGA AAAAGCAGAG CACGAGAGAG AACTGGCCCT GCGGAACGAG
    CTTATTCGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TCGAGGCCGC CACGAAAAAG CACGAGGCCA TTGAGACGGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTTGCC GTGGCCAGGG AGCTGGAAGC GGAGAACTAC
    CATGACATCA AGCGCATCAC GGCAAGGAAG GACAACGTCA TCCGGCTCTG GGAATACCTG
    CTGGAACTGC TCAGGGCCCG GAGGCAGCGC CTCGAGATGA ACCTGGGCCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT ATTACTGTCT
    CAGGACTATG GCAAACACTT GCTGGGCGTG GAAGACCTGT TGCAGAAGCA TGCCCTGGTT
    GAAGCGGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC TGCCCAGAAG
    TTTGCAACAG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGTTGG CAGCAGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGGCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAGGA GGAAGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GCAAGGACCT CACCAGTGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGACGAGA TGAGCGGCCG CAGCGGTCAC
    TTCGAGCAGG CCATCAAAGA GGGCGAGGAC ATGATCGCTG AGGAGCACTT TGGGTCGGAG
    AAGATCCGGG AGCGGATCGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCACTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ACGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCTCTGGTC AAGAAGCACA AGGATGTGGC CGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAGG CCGGTGCCCT CCCCCAGGAG
    CACGCCGAGT CCCCCGATGT ACGGGGCCGG CTCGCGGGCA TCGAGGAGCG CTACAAGGAG
    GTGGCTGAAC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGCGAGCTC TGGATCGATG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    TTAGAACCAG AGATGAACAA CCAAGCTTCC CGAGTGGCGG TGGTGAATCA GATTGCACGG
    CAGCTGATGC ACAGCGGCCA CCCAAGCGAG AAGGAGATCA AGGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGTCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC ACTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAGTCCTG GATCCGGGAA
    AAGACCAAGG TCATCGAATC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    TTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATCGAGGC GAAGCTGAGC
    GACCTGCAGA AGGAGGCTGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAAACCAC GCTGAGGAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCAG GACCCAGACG GCCATCGCCT CGGAGGACAT GCCCAACACC
    CTGACCGAAG CCGAGAAGCT CCTCACACAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGTGAGA TGGTCACGCA GGGCCAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAATGAG
    CTCCACAAGA TGTGGGAGAA CAGACAGAAC CTCCTTTCCC AGTCCCACGC CCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCTGAAGCC TTTCTCAACA ACCAGGAGTA TGTCTTGGCC
    CATACTGAGA TGCCCACCAC CTTGGAAGGG GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CAGTCGTGGA GACCGGCCGC
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AAGAGAAGGT GGACTCCATC
    GATGACAGAC ATAGGAAGAA TCGCGAAGCA GCAAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAAGAGT TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTC GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCGGAGA CGGAAGCCGT GGTAAAGGAG
    AAACTCACAG GTTTGCATAA AATGTGGGAA GTCCTTGAAT CCACCACGCA GACCAAGGCC
    CAGCGGCTCT TCGATGCAAA CAAGGCTGAA CTCTTCACCC AGAGCTGTGC AGACCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAATCTGATG ATTACGGCAA AGACCTCACC
    AGTGTCAACA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATTG AGGAGCTCCA GAGCCAGGCA CAGGCCCTGA GCCAGGAGGG GAAGAGCACA
    GACGAGGTGG ACAGCAAGCG GCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTAAATGAGA GGAAGCAGAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT TAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAATC TCCAGACCGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATTTTCGAGA GGAGCCAGAA CATCGTTGCC
    GACAGCAGCA GCCTCAGTGC CGAGGCCATC CGGCAGAGGC TGGCCGACCT GAAGCAGCTG
    TGGGGCCAGC TCATCGAGGA GACGGAGAAG CGGCACCGGC GGCTGGAGGA GGCACACAGG
    GCCCAGCAGT ACTACTTCGA TGCTGCCGAG GCCGAGGCGT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CAGTCTCTAT GTTGAAGAAG
    CACCAGATTC TGGAACAAGC AGTGGAGGAC TACGCAGAGA CGGTACATCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTAGC CGACAGCCAT CCTGAAAGCG AGCGCATCAG CATGCGGCAA
    TCCAAGGTGG ACAAGCTGTA CGCGGGCCTC AAGGACCTGG CCGAGGAGAG GAGGGGCAAG
    CTGGACGAGA GGCACCGGCT GTTCCAGCTC AACCGCGAGG TGGACGACCT GGAGCAGTGG
    ATTGCCGAGA GGGAGGTGGT CGCAGGGTCT CACGAGTTGG GGCAGGACTA CGAGCATGTC
    ACGATGCTAC AAGAACGGTT CCGGGAGTTT GCCCGAGACA CGGGAAACAT CGGGCAGGAG
    CGCGTGGACA CTGTCAATCA CATGGCAGAT GAGCTCATCA ACTCCGGACA TTCAGATGCT
    GCCACCATTG CCGAGTGGAA GGACGGGCTC AACGAAGCCT GGGCAGACCT CCTGGAGCTC
    ATTGACACGA GAACACAGAT CCTTGCCGCC TCCTACGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGACG CATCCAGGAC AAACACAAGA AACTCCCCGA GGAGCTCGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCATA CCACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGACG CAGCCCGCCT CCAGGCAGCC
    TATGCAGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ACGAGGTCCT AGAAGCCTGG
    AAGGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTATGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT CGATGCTCGA AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAGTCGC TGCTGGCGAG GAAGCACTAC GCGTCAGAGG AGATCAAGGA GAAGTTACTG
    CAGTTGACGG ACAAGAGAAA AGAAATGATT GACAAATGGG AAGACCGATG GGAGTGGTTA
    AGACTGATCT TGGAGGTCCA TCAGTTCTCA AGGGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAAATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCATGA GGCTTTTGAA AAGTCTGCAG CGACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AACGGTTGAC GACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCCAGCA CAAAGGTTTC CGAGGAGACC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA TGGTTTGCCG
    ACCGAGCAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CGAGTGAAAT GGTCAACGGC
    GCCGCAGAAC AGAGGACAAG CTCGAAGGAG TCCAGCCCCA TCCCCTCCCC AACCTCCGAC
    CGCAAAGCCA AGACCTCCCT CCCTGCCCAG AGTGCCGCCA CCTTGCCGGC CAGAACCCAG
    GAGACGCCCT CGGCCCAGAT GGAAGGCTTC CTGAATCGGA AGCACGAGTG GGAGGCCCAC
    AACAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTACT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGACGC AAAGACAGCT GCTTCCGGAA TTCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTCG ATTACAAAAA GAAGAAACAC
    GTGTTCAAGC TAAGACTAAA TGATGGCAAC GAGTACCTCT TCCAAGCCAA AGATGATGAG
    GAAATGAACA CGTGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCCGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCTC AGACCTTGCC CACCAGCGCC
    GTGACCATCA CCAGCGAGTC CAGTCCCGGC AAACGGGAGA AGGACAAAGA GAAAGACAAG
    GAAAAGAGGT TCAGCCTTTT TGGCAAGAAG AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe246961
    Clone ID Related Accession (Same CDS sequence) XM_021088224.1
    Accession Version XM_021088224.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product spectrin beta chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010445.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    GATAACATCA TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020943883.1
    CDS278..6712
    Translation

    Target ORF information:

    RefSeq Version XM_021088224.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021088224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGACGACCA CAGTAGCCAC AGACTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GATAATGAGA ACAGCTCCGC GAGGCTCTTT
    GAGCGATCGC GCATCAAGGC TCTGGCAGAT GAGCGCGAAG CCGTGCAAAA GAAGACCTTT
    ACCAAGTGGG TCAACTCCCA CCTCGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACC
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGACTG
    CCCAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTG GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGGTT CCAGATCCAG
    GACATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ACATTCACAA TTTCACTACC
    AGCTGGAGGG ACGGCATGGC CTTCAACGCC CTGATACACA AGCACAGGCC TGACCTGATA
    GATTTTGACA AACTGAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAACTTG
    GCGGAGCAGC ACCTTGGCCT CACCAAACTG TTGGACCCTG AAGATATCAG CGTGGACCAC
    CCCGATGAGA AGTCCATTAT CACTTACGTG GTGACTTATT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TTGACAATGC TATTGAAACT
    GAAAAAATGA TTGAAAAGTA TGAATCCCTT GCCTCTGATC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCGC TGGTTGGGGT CCAACAACAG
    CTTCAGGCCT TCAACACTTA CCGCACTGTG GAGAAACCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACTATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GATATTAACA AGGCCTGGGA ACGACTGGAA
    AAAGCAGAGC ACGAGAGAGA ACTGGCCCTG CGGAACGAGC TTATTCGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT CGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT CGAGGCCGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACGGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTTGCCG TGGCCAGGGA GCTGGAAGCG GAGAACTACC ATGACATCAA GCGCATCACG
    GCAAGGAAGG ACAACGTCAT CCGGCTCTGG GAATACCTGC TGGAACTGCT CAGGGCCCGG
    AGGCAGCGCC TCGAGATGAA CCTGGGCCTG CAGAAGATAT TCCAGGAAAT GCTGTACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTA TTACTGTCTC AGGACTATGG CAAACACTTG
    CTGGGCGTGG AAGACCTGTT GCAGAAGCAT GCCCTGGTTG AAGCGGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TTGCAACAGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGTTGGC AGCAGAGCGC CGGGCCCGCC TGGAAGAGTC CCGGCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG CAAGGACCTC ACCAGTGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGACGAGAT GAGCGGCCGC AGCGGTCACT TCGAGCAGGC CATCAAAGAG
    GGCGAGGACA TGATCGCTGA GGAGCACTTT GGGTCGGAGA AGATCCGGGA GCGGATCGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CACTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA CGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCTCTGGTCA AGAAGCACAA GGATGTGGCC GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAGGC CGGTGCCCTC CCCCAGGAGC ACGCCGAGTC CCCCGATGTA
    CGGGGCCGGC TCGCGGGCAT CGAGGAGCGC TACAAGGAGG TGGCTGAACT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGCGAGCTCT GGATCGATGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCT TAGAACCAGA GATGAACAAC
    CAAGCTTCCC GAGTGGCGGT GGTGAATCAG ATTGCACGGC AGCTGATGCA CAGCGGCCAC
    CCAAGCGAGA AGGAGATCAA GGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGTCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCA CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAGTCCTGG ATCCGGGAAA AGACCAAGGT CATCGAATCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCT TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATCGAGGCG AAGCTGAGCG ACCTGCAGAA GGAGGCTGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAAACCACG CTGAGGAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCAGG
    ACCCAGACGG CCATCGCCTC GGAGGACATG CCCAACACCC TGACCGAAGC CGAGAAGCTC
    CTCACACAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGTGAGAT GGTCACGCAG GGCCAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAATGAGC TCCACAAGAT GTGGGAGAAC
    AGACAGAACC TCCTTTCCCA GTCCCACGCC CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCTGAAGCCT TTCTCAACAA CCAGGAGTAT GTCTTGGCCC ATACTGAGAT GCCCACCACC
    TTGGAAGGGG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC AGTCGTGGAG ACCGGCCGCA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA AGAGAAGGTG GACTCCATCG ATGACAGACA TAGGAAGAAT
    CGCGAAGCAG CAAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAAGAGTT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTCG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCGGAGAC GGAAGCCGTG GTAAAGGAGA AACTCACAGG TTTGCATAAA
    ATGTGGGAAG TCCTTGAATC CACCACGCAG ACCAAGGCCC AGCGGCTCTT CGATGCAAAC
    AAGGCTGAAC TCTTCACCCA GAGCTGTGCA GACCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AATCTGATGA TTACGGCAAA GACCTCACCA GTGTCAACAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATTGA GGAGCTCCAG
    AGCCAGGCAC AGGCCCTGAG CCAGGAGGGG AAGAGCACAG ACGAGGTGGA CAGCAAGCGG
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TAAATGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTT AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAATCT CCAGACCGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATTGATGACA TTTTCGAGAG GAGCCAGAAC ATCGTTGCCG ACAGCAGCAG CCTCAGTGCC
    GAGGCCATCC GGCAGAGGCT GGCCGACCTG AAGCAGCTGT GGGGCCAGCT CATCGAGGAG
    ACGGAGAAGC GGCACCGGCG GCTGGAGGAG GCACACAGGG CCCAGCAGTA CTACTTCGAT
    GCTGCCGAGG CCGAGGCGTG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC AGTCTCTATG TTGAAGAAGC ACCAGATTCT GGAACAAGCA
    GTGGAGGACT ACGCAGAGAC GGTACATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTAGCC
    GACAGCCATC CTGAAAGCGA GCGCATCAGC ATGCGGCAAT CCAAGGTGGA CAAGCTGTAC
    GCGGGCCTCA AGGACCTGGC CGAGGAGAGG AGGGGCAAGC TGGACGAGAG GCACCGGCTG
    TTCCAGCTCA ACCGCGAGGT GGACGACCTG GAGCAGTGGA TTGCCGAGAG GGAGGTGGTC
    GCAGGGTCTC ACGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGCTACA AGAACGGTTC
    CGGGAGTTTG CCCGAGACAC GGGAAACATC GGGCAGGAGC GCGTGGACAC TGTCAATCAC
    ATGGCAGATG AGCTCATCAA CTCCGGACAT TCAGATGCTG CCACCATTGC CGAGTGGAAG
    GACGGGCTCA ACGAAGCCTG GGCAGACCTC CTGGAGCTCA TTGACACGAG AACACAGATC
    CTTGCCGCCT CCTACGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGACGC
    ATCCAGGACA AACACAAGAA ACTCCCCGAG GAGCTCGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCATAC CACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGACGC AGCCCGCCTC CAGGCAGCCT ATGCAGGTGA CAAGGCTGAC
    GACATCCAGA AGCGGGAGAA CGAGGTCCTA GAAGCCTGGA AGGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTATGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGTATCAA AGCTGAAATC
    GATGCTCGAA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAGTCGCT GCTGGCGAGG
    AAGCACTACG CGTCAGAGGA GATCAAGGAG AAGTTACTGC AGTTGACGGA CAAGAGAAAA
    GAAATGATTG ACAAATGGGA AGACCGATGG GAGTGGTTAA GACTGATCTT GGAGGTCCAT
    CAGTTCTCAA GGGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AAATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCATGAG
    GCTTTTGAAA AGTCTGCAGC GACCTGGGAC GAGAGGTTCT CCGCCCTGGA ACGGTTGACG
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCCAGCAC AAAGGTTTCC GAGGAGACCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAT GGTTTGCCGA CCGAGCAGGG ATCTCCACGG
    GATAACATCA TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.