UBE4A cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	100513674
Full Name	ubiquitination factor E4A
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244872.1	NP_001231801.1	ubiquitin conjugation factor E4 A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
Gorodkin J, Cirera S, Hedegaard J, Gilchrist MJ, Panitz F, J?rgensen C, Scheibye-Knudsen K, Arvin T, Lumholdt S, Sawera M, Green T, Nielsen BJ, Havgaard JH, Rosenkilde C, Wang J, Li H, Li R, Liu B, Hu S, Dong W, Li W, Yu J, Wang J, Staefeldt HH, Wernersson R, Madsen LB, Thomsen B, Hornsh?j H, Bujie Z, Wang X, Wang X, Bolund L, Brunak S, Yang H, Bendixen C, Fredholm M
Genome biology8(4)R45(2007)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe29530	NM_001244872.1 Latest version!	Sus scrofa ubiquitination factor E4A (UBE4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( NM_001244872.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001231801.1 Sus scrofa ubiquitination factor E4A (UBE4A), mRNA.

Price & Availability↑

CloneID	OSe29530
Clone ID Related Accession (Same CDS sequence)	NM_001244872.1
Accession Version	NM_001244872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-28
Organism	Sus scrofa(pig)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK344471.1, AJ681917.1, EW577030.2, FS666363.1, BX920097.2 and EW248448.2. On Oct 13, 2011 this sequence version replaced XM_003129907.2. Sequence Note: This RefSeq record was created from transcripts from different breeds because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END##

ATGACAGACC AGGAGAATAA CAACAACATT TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA ATTTGCAGCA ATCCAAAAGG AGCAGCTGAA GCAGCAGTCT 
GATGAACTCC CAGCCAGCCC GGATGACTCA GATAATAGTG TGTCAGAGAG CCTGGATGAA 
TTTGATTACT CCGTGGCTGA GAGTAGCCGC TCATTCCGTT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAC 
CCAAGCCTGA AAAGTGGGAA TGGCATCCCC AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGACCTGG AAGATCAGGA CTGGCTTGAT ATGAACAACG TAGAGCAGGC TGTCTTCACT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTGATTAACA TGACTTCTTC TACAACGTTG 
AATCTCTCTG CTGATCGAGA TGCAGGGGAG AGGCACATTT TTTGTTACCT TTATTCCTGC 
TTCCAAAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGGA ATCTCACTGT GTCCAATACC CGAACGGTTC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCACGA GCAACTGGTA GATTTGATGT TGGAAGCCAT CCTAGGAGGC 
CATTTTGAAA GTGTAGCTGA ATTTCTGGAT GAGATCATCG GAGCCTTGAT ACTGGATGAG 
GAAGTTCGAA CATTTTCAGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG ACCTCTGCCA GATCCTGTTG TATGCATATC TGGACATTCT TCTCTATTTT 
ACTAGGCAAA AGGATATGGC AAAGGTTTTT GTAGAATACA TTCAGCCCAA GGACCCTAAT 
AATGGACAGA TGTACCAGAA GACCTTGCTG GGAGTAATCC TGAATATTTC CTGCTTATTA 
GAGACTCCGG GTGTGGTAGA AAATCATGGA TACTTCCTGA ATCCATCTCG TTCCAGTCCC 
CAGGAGATCA AAGTACAGGA GGCCAATATT CATCAGTTCA TGGCTCGGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTGTGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACTT ACTGTGCTCT GAAGGAGTTG AATGATGAAG AACGAAAAAT AAAAAATGTA 
CACATGAGAG GTTTGGACAA AGAAACCTGT TTGATCCCAG CTGTGCAGGA GCCAAAATTT 
CCCCAGAACT ACAACCTCGT TACAGAGAAC CTTGTCCTGA CAGAGTACAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
ATAGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAACCT CCGTGAGCAG 
TTTGAACGAC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACTGA GCCACAGATG 
CTACAAAACT GCCTAAATTT GCAGGTGTCC ATGGCTGTTC TCTTAGTTCA GCTGGCCATC 
GGCAATGAGG GCTCACAGCC AATAGAGCTA ACTTTCCCTT TGCCAGATGG CTACAGTTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACTTGGGTG ATTTCCTCAT TTTTCTCCGC 
CGCTTTGCCG ATGACATCTT GGAGACATCA GCAGATTCTC TGGAACATGT CCTTCACTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGG ATGAAGAATC CCCACTTAAG GGCCAAACTG 
GCAGAGGTGT TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTGCCT ATGCAGCCCA CCTTGCAGAA 
GCTCTAATCA AGGTCTTTGT GGACATTGAG TTTACAGGAG ACCCCCATCA ATTTGAGCAG 
AAGTTTAATT ACCGCCGTCC CATGTATCCC ATCCTCAGGT ACATGTGGGG CACAGATAAC 
TATCGAGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGCTT TCTTAACCTG CTAATGAATG ATGCCATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAGA TTGAGAAGGA CCGTGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAAAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGTACCCT TGCTTTTCTG 
ACATCAGAGA TCAAATCACT CTTTGTGCAC CCTTTCCTGG CTGAGCGCAT CATCTCCATG 
CTGAACTACT TTCTACAGCA TCTGGTTGGC CCCAAAATGG GGGCCTTAAA AGTCAAGGAT 
TTCAGTGAAT TTGACTTCAA GCCCCAGCAG CTCGTTTCAG ATATCTGCAC CATCTACCTA 
AATCTCGGGG ATGAGGAGAA CTTCTGTGCC ACCGTGCCCA AGGATGGACG CTCCTATTCC 
CAAACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TAAATAAGCC TGGAAATATG 
ATTGTGGCTT TCAGCAACTT AGCAGAGAGA ATCAAGTCTC TGGCAGACCT CCAGCAACAG 
GAAGAGGAGA CCTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATCAT GAGCACACTG 
ATGTCTGACC CTGTGGTGCT GCCATCTTCC AGAGTCACTG TGGATAGATC CACCATTGCC 
AGACATTTGC TCAGTGACCA AACAGATCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGGT GGCTCGCAGA GAGGAAGCAA 
CAACAAAAGG AGCAACTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231801.1
CDS	116..3319
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAESSRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVDLEDQDWLDMNNVEQAVFTRLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAILGGHFESVAEFLDEIIGALILDEEVRTFSEVMIPVFDILLGRIKDLDLCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPNNGQMYQKTLLGVILNISCLLETPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMARFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLVLTEYTLYLGFHRLHDQMVKINQNLHRLQIAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFAYAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDNYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSQTLFAQTVRVLKKINKPGNMIVAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQQKEQLE

Target ORF information:

RefSeq Version	NM_001244872.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa ubiquitination factor E4A (UBE4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244872.1

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATT TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA ATTTGCAGCA ATCCAAAAGG AGCAGCTGAA GCAGCAGTCT 
GATGAACTCC CAGCCAGCCC GGATGACTCA GATAATAGTG TGTCAGAGAG CCTGGATGAA 
TTTGATTACT CCGTGGCTGA GAGTAGCCGC TCATTCCGTT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAC 
CCAAGCCTGA AAAGTGGGAA TGGCATCCCC AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGACCTGG AAGATCAGGA CTGGCTTGAT ATGAACAACG TAGAGCAGGC TGTCTTCACT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTGATTAACA TGACTTCTTC TACAACGTTG 
AATCTCTCTG CTGATCGAGA TGCAGGGGAG AGGCACATTT TTTGTTACCT TTATTCCTGC 
TTCCAAAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGGA ATCTCACTGT GTCCAATACC CGAACGGTTC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCACGA GCAACTGGTA GATTTGATGT TGGAAGCCAT CCTAGGAGGC 
CATTTTGAAA GTGTAGCTGA ATTTCTGGAT GAGATCATCG GAGCCTTGAT ACTGGATGAG 
GAAGTTCGAA CATTTTCAGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG ACCTCTGCCA GATCCTGTTG TATGCATATC TGGACATTCT TCTCTATTTT 
ACTAGGCAAA AGGATATGGC AAAGGTTTTT GTAGAATACA TTCAGCCCAA GGACCCTAAT 
AATGGACAGA TGTACCAGAA GACCTTGCTG GGAGTAATCC TGAATATTTC CTGCTTATTA 
GAGACTCCGG GTGTGGTAGA AAATCATGGA TACTTCCTGA ATCCATCTCG TTCCAGTCCC 
CAGGAGATCA AAGTACAGGA GGCCAATATT CATCAGTTCA TGGCTCGGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTGTGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACTT ACTGTGCTCT GAAGGAGTTG AATGATGAAG AACGAAAAAT AAAAAATGTA 
CACATGAGAG GTTTGGACAA AGAAACCTGT TTGATCCCAG CTGTGCAGGA GCCAAAATTT 
CCCCAGAACT ACAACCTCGT TACAGAGAAC CTTGTCCTGA CAGAGTACAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
ATAGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAACCT CCGTGAGCAG 
TTTGAACGAC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACTGA GCCACAGATG 
CTACAAAACT GCCTAAATTT GCAGGTGTCC ATGGCTGTTC TCTTAGTTCA GCTGGCCATC 
GGCAATGAGG GCTCACAGCC AATAGAGCTA ACTTTCCCTT TGCCAGATGG CTACAGTTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACTTGGGTG ATTTCCTCAT TTTTCTCCGC 
CGCTTTGCCG ATGACATCTT GGAGACATCA GCAGATTCTC TGGAACATGT CCTTCACTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGG ATGAAGAATC CCCACTTAAG GGCCAAACTG 
GCAGAGGTGT TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTGCCT ATGCAGCCCA CCTTGCAGAA 
GCTCTAATCA AGGTCTTTGT GGACATTGAG TTTACAGGAG ACCCCCATCA ATTTGAGCAG 
AAGTTTAATT ACCGCCGTCC CATGTATCCC ATCCTCAGGT ACATGTGGGG CACAGATAAC 
TATCGAGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGCTT TCTTAACCTG CTAATGAATG ATGCCATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAGA TTGAGAAGGA CCGTGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAAAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGTACCCT TGCTTTTCTG 
ACATCAGAGA TCAAATCACT CTTTGTGCAC CCTTTCCTGG CTGAGCGCAT CATCTCCATG 
CTGAACTACT TTCTACAGCA TCTGGTTGGC CCCAAAATGG GGGCCTTAAA AGTCAAGGAT 
TTCAGTGAAT TTGACTTCAA GCCCCAGCAG CTCGTTTCAG ATATCTGCAC CATCTACCTA 
AATCTCGGGG ATGAGGAGAA CTTCTGTGCC ACCGTGCCCA AGGATGGACG CTCCTATTCC 
CAAACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TAAATAAGCC TGGAAATATG 
ATTGTGGCTT TCAGCAACTT AGCAGAGAGA ATCAAGTCTC TGGCAGACCT CCAGCAACAG 
GAAGAGGAGA CCTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATCAT GAGCACACTG 
ATGTCTGACC CTGTGGTGCT GCCATCTTCC AGAGTCACTG TGGATAGATC CACCATTGCC 
AGACATTTGC TCAGTGACCA AACAGATCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGGT GGCTCGCAGA GAGGAAGCAA 
CAACAAAAGG AGCAACTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
Genome biology8(4)R45(2007)
Gorodkin J,Cirera S,Hedegaard J,Gilchrist MJ,Panitz F,J?rgensen C,Scheibye-Knudsen K,Arvin T,Lumholdt S,Sawera M,Green T,Nielsen BJ,Havgaard JH,Rosenkilde C,Wang J,Li H,Li R,Liu B,Hu S,Dong W,Li W,Yu J,Wang J,Staefeldt HH,Wernersson R,Madsen LB,Thomsen B,Hornsh?j H,Bujie Z,Wang X,Wang X,Bolund L,Brunak S,Yang H,Bendixen C,Fredholm M

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

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UBE4A cDNA ORF clone, Sus scrofa(pig)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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