SLC12A1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A1
Entrez Gene ID	100154291
Full Name	solute carrier family 12 member 1
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 1 Map Location: 1\|1

mRNA and Protein(s)

mRNA	Protein	Name
XM_013985455.1	XP_013840909.1	solute carrier family 12 member 1 isoform X1
XM_005654446.3	XP_005654503.1	solute carrier family 12 member 1 isoform X2
XM_001926142.6	XP_001926177.4	solute carrier family 12 member 1 isoform X2
XM_013985468.1	XP_013840922.1	solute carrier family 12 member 1 isoform X5
XM_005654446.2	XP_005654503.1	solute carrier family 12 member 1 isoform X3
XM_005654445.2	XP_005654502.1	solute carrier family 12 member 1 isoform X4
XM_001926142.7	XP_001926177.4	solute carrier family 12 member 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following SLC12A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe02278	XM_001926142.7 Latest version!	Sus scrofa solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe02279	XM_005654445.2 Latest version!	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe02280	XM_005654446.3 Latest version!	Sus scrofa solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe31175	XM_013985455.1 Latest version!	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe31176	XM_013985468.1 Latest version!	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSe02280	XM_005654446.2	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe02278	XM_001926142.6	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A1 ( XM_001926142.6 ) cDNA ORF clone, Sus scrofa(pig) -> XP_001926177.4 Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe02278
Clone ID Related Accession (Same CDS sequence)	XM_001926142.6 , XM_001926142.7
Accession Version	XM_001926142.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	solute carrier family 12 member 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534009.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_001926142.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001926177.4
CDS	3468..6770
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_001926142.6
Organism	Sus scrofa(Pig)
Definition	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001926142.6

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_001926142.7 ) cDNA ORF clone, Sus scrofa(pig) -> XP_001926177.4 Sus scrofa solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe02278
Clone ID Related Accession (Same CDS sequence)	XM_001926142.6 , XM_001926142.7
Accession Version	XM_001926142.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_001926142.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001926177.4
CDS	243..3545
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_001926142.7
Organism	Sus scrofa(pig)
Definition	Sus scrofa solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001926142.7

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005654445.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005654502.1 Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe02279
Clone ID Related Accession (Same CDS sequence)	XM_005654445.2
Accession Version	XM_005654445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	solute carrier family 12 member 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534009.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005654445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGTG TGATTATCAT CGGCTTAGCC 
GTGACAGTGA CTGGTATCAC TGGTTTATCA ACCTCTGCTA TTGCCACAAA TGGATATGTC 
AGAGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005654502.1
CDS	3468..6770
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTGITGLSTSAIATNGYVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005654445.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005654445.2

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGTG TGATTATCAT CGGCTTAGCC 
GTGACAGTGA CTGGTATCAC TGGTTTATCA ACCTCTGCTA TTGCCACAAA TGGATATGTC 
AGAGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005654446.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005654503.1 Sus scrofa solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe02280
Clone ID Related Accession (Same CDS sequence)	XM_005654446.3 , XM_005654446.2
Accession Version	XM_005654446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005654446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TCATCATCAT TGGCCTGAGT 
GTGGTCGTGA CAACACTCAC AGGTATTTCT ATGTCTGCTA TTTGCACAAA TGGAGTAGTA 
AGAGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005654503.1
CDS	333..3635
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005654446.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005654446.3

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TCATCATCAT TGGCCTGAGT 
GTGGTCGTGA CAACACTCAC AGGTATTTCT ATGTCTGCTA TTTGCACAAA TGGAGTAGTA 
AGAGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005654446.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005654503.1 Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.

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CloneID	OSe02280
Clone ID Related Accession (Same CDS sequence)	XM_005654446.3 , XM_005654446.2
Accession Version	XM_005654446.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	solute carrier family 12 member 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534009.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005654446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TCATCATCAT TGGCCTGAGT 
GTGGTCGTGA CAACACTCAC AGGTATTTCT ATGTCTGCTA TTTGCACAAA TGGAGTAGTA 
AGAGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005654503.1
CDS	3468..6770
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005654446.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005654446.2

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TCATCATCAT TGGCCTGAGT 
GTGGTCGTGA CAACACTCAC AGGTATTTCT ATGTCTGCTA TTTGCACAAA TGGAGTAGTA 
AGAGGAGGTG GGGCTTACTA CCTTATTTCC AGAAGCTTAG GGCCCGAGTT TGGTGGGTCT 
ATAGGCTTGA TCTTTGCCTT TGCCAATGCA GTGGCTGTTG CTATGTACGT GGTGGGATTT 
GCTGAGACTG TGGTAGATCT CCTTAAGGAG AGTGATTCGA TGATGGTGGA TCCAACCAAT 
GACATCCGGA TAATAGGCTC CATCACAGTG GTGATTCTTT TAGGAATTTC AGTAGCTGGA 
ATGGAATGGG AAGCGAAGGC TCAAGTGATT CTTCTGGTCA TTCTTCTCAT TGCTATTGCA 
AACTTCTTCA TTGGAACTGT CATTCCATCC AACAGTGAGA AAAAGTCCAG AGGTTTCTTT 
AATTATCAAG CATCAATATT TGCAGAAAAC TTTGGGCCAA GTTTCACAAA GGGTGAAGGC 
TTCTTCTCCG TCTTTGCCAT TTTTTTCCCA GCAGCAACTG GGATTCTAGC TGGTGCCAAT 
ATCTCAGGAG ATTTAGAGGA TCCCCAAGAC GCCATCCCCA AAGGGACGAT GCTGGCCATC 
TTCATCACTA CTGTTGCCTA CATAGGAGTT GCTATTTGTG TAGGGGCCTG TGTGGTCAGA 
GATGCCACTG GAAGCATGAA TGACACCATC ATTTCTGGGA TGAATTGCAA TGGTTCAGCA 
GCCTGTGGGT TAGGCTACGA TTTCTCAAGA TGTCGGCATG AACCATGTCA ATATGGGCTG 
ATGAACAATT TCCAGGTCAT GAGCATGGTG TCAGGGTTTG GCCCCCTCAT CACTGCTGGC 
ATCTTTTCGG CGACACTCTC CTCGGCCCTG GCCTCCCTTG TCAGCGCACC CAAAGTGTTC 
CAGGCTCTGT GCAAGGACAA CATCTACAAA GCCCTGCAGT TCTTTGCAAA GGGATATGGG 
AAAAACAATG AACCCTTGAG AGGATACTTT CTCACTTTTG TTATAGCCAT GGCGTTCATT 
CTTATTGCGG AACTGAATAC CATTGCTCCC ATCATTTCCA ACTTTTTCCT CGCCTCATAT 
GCACTTATTA ATTTCTCCTG CTTCCATGCT TCTTATGCCA AATCTCCAGG ATGGAGACCT 
GCATATGGTA TTTACAACAT GTGGGTATCT CTTTTTGGAG CTATTTTGTG CTGTGCAGTC 
ATGTTTGTCA TCAACTGGTG GGCAGCTGTC ATTACCTATG TCATTGAGTT CTTCCTCTAT 
ATCTATGTGA CTTATAAGAA GCCAGATGTG AACTGGGGCT CCTCCACGCA GGCTCTTTCC 
TACGTGAGCG CTTTAGACAA TGCTTTGGAA CTAACTACTG TGGAAGACCA TGTGAAAAAT 
TTCAGGCCCC AGTGCATTGT CTTAACAGGG GGGCCCATGT CAAGACCTGC TCTCTTGGAT 
ATAACTCACG CCTTCACCAA GAACAGTGGC CTTTGCATCT GCTGTGAAGT CTTTGTGGGA 
CCGCGCAAGC TGTGTGTTAA AGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ATAAAGAACA AAATCAAGGC TTTCTATGCT GCAGTGGCAG CAGATTGTTT CAGGGATGGA 
GTTAGAAGCC TCCTTCAGGC CTCGGGCTTA GGAAGAATGA AACCAAACAC TCTGGTGATT 
GGATATAAAA AAAACTGGAG AAAAGCTCCC CTGACAGAGA TTGAAAACTA CGTGGGAATC 
ATACATGATG CATTTGATTT TGAGATTGGT GTTGTCATAG TCAGAATCAG CCAAGGATTT 
GACATCTCCC ATGTTCTTCA GGTGCAAGAT GAGTTGGAGA AATTAGAACA GGAGAGGCTG 
GCTTTGGAAG CCACTATCAA AGACAATGAA TGTGAAGAAG GAAGTGGAGG CATCCGAGGC 
TTATTTAAAA AAGCTGGCAA GCTGAACATT ACTAAGACAA CTCCTAAAAA AGACAGCAGT 
ATTAACACAA TCCAGTCGAT GCATGTGGGG GAGTTCAACC AGAAACTGGT AGAAGCCAGC 
ACCCAATTTA AAAAGAAGCA AGGAAAAGGC ACAATTGATG TTTGGTGGTT GTTTGATGAT 
GGAGGGTTAA CACTCCTTAT CCCCTATATC TTGACTCTCA GAAAAAAATG GAAAGACTGT 
AAATTAAGAA TCTATGTTGG AGGGAAGATC AATCGCATTG AAGAAGAAAA AATTGCAATG 
GCTTCCCTTC TGAGCAAATT TAGGATAAAA TTTGCAGACA TCCATATCAT TGGTGACATC 
AACATTAAGC CAAACAAAGA AAGTTGGAAA GTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCATGAAA GCTGCAAAGA TTTAACAACT GCTGAGAAAT TAAAGAGAGA AACTCCGTGG 
AAAATTACAG ATGCAGAACT GGAAGCAGTC AAGGAAAAGA GTTACCGCCA AGTTCGACTG 
AATGAACTCT TGCAAGAGCA CTCAAGAGCT GCCAATCTCA TAGTTCTGAG CCTTCCAGTG 
GCAAGAAAAG GATCTATATC AGATTGGTTG TACATGGCTT GGTTGGAAAT CCTCACGAAG 
AACCTCCCTC CTGTCTTACT AGTTAGAGGA AATCATAAAA ATGTCCTGAC ATTTTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_013985455.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840909.1 Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe31175
Clone ID Related Accession (Same CDS sequence)	XM_013985455.1
Accession Version	XM_013985455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	solute carrier family 12 member 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534009.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTC TTGGAGTCAT CATCATTGGC CTGAGTGTGG TCGTGACAAC ACTCACAGGT 
ATTTCTATGT CTGCTATTTG CACAAATGGA GTAGTAAGAG GAGGTGGGGC TTACTACCTT 
ATTTCCAGAA GCTTAGGGCC CGAGTTTGGT GGGTCTATAG GCTTGATCTT TGCCTTTGCC 
AATGCAGTGG CTGTTGCTAT GTACGTGGTG GGATTTGCTG AGACTGTGGT AGATCTCCTT 
AAGGAGAGTG ATTCGATGAT GGTGGATCCA ACCAATGACA TCCGGATAAT AGGCTCCATC 
ACAGTGGTGA TTCTTTTAGG AATTTCAGTA GCTGGAATGG AATGGGAAGC GAAGGCTCAA 
GTGATTCTTC TGGTCATTCT TCTCATTGCT ATTGCAAACT TCTTCATTGG AACTGTCATT 
CCATCCAACA GTGAGAAAAA GTCCAGAGGT TTCTTTAATT ATCAAGCATC AATATTTGCA 
GAAAACTTTG GGCCAAGTTT CACAAAGGGT GAAGGCTTCT TCTCCGTCTT TGCCATTTTT 
TTCCCAGCAG CAACTGGGAT TCTAGCTGGT GCCAATATCT CAGGAGATTT AGAGGATCCC 
CAAGACGCCA TCCCCAAAGG GACGATGCTG GCCATCTTCA TCACTACTGT TGCCTACATA 
GGAGTTGCTA TTTGTGTAGG GGCCTGTGTG GTCAGAGATG CCACTGGAAG CATGAATGAC 
ACCATCATTT CTGGGATGAA TTGCAATGGT TCAGCAGCCT GTGGGTTAGG CTACGATTTC 
TCAAGATGTC GGCATGAACC ATGTCAATAT GGGCTGATGA ACAATTTCCA GGTCATGAGC 
ATGGTGTCAG GGTTTGGCCC CCTCATCACT GCTGGCATCT TTTCGGCGAC ACTCTCCTCG 
GCCCTGGCCT CCCTTGTCAG CGCACCCAAA GTGTTCCAGG CTCTGTGCAA GGACAACATC 
TACAAAGCCC TGCAGTTCTT TGCAAAGGGA TATGGGAAAA ACAATGAACC CTTGAGAGGA 
TACTTTCTCA CTTTTGTTAT AGCCATGGCG TTCATTCTTA TTGCGGAACT GAATACCATT 
GCTCCCATCA TTTCCAACTT TTTCCTCGCC TCATATGCAC TTATTAATTT CTCCTGCTTC 
CATGCTTCTT ATGCCAAATC TCCAGGATGG AGACCTGCAT ATGGTATTTA CAACATGTGG 
GTATCTCTTT TTGGAGCTAT TTTGTGCTGT GCAGTCATGT TTGTCATCAA CTGGTGGGCA 
GCTGTCATTA CCTATGTCAT TGAGTTCTTC CTCTATATCT ATGTGACTTA TAAGAAGCCA 
GATGTGAACT GGGGCTCCTC CACGCAGGCT CTTTCCTACG TGAGCGCTTT AGACAATGCT 
TTGGAACTAA CTACTGTGGA AGACCATGTG AAAAATTTCA GGCCCCAGTG CATTGTCTTA 
ACAGGGGGGC CCATGTCAAG ACCTGCTCTC TTGGATATAA CTCACGCCTT CACCAAGAAC 
AGTGGCCTTT GCATCTGCTG TGAAGTCTTT GTGGGACCGC GCAAGCTGTG TGTTAAAGAG 
ATGAACAGTG GCATGGCAAA AAAGCAGGCC TGGCTTATAA AGAACAAAAT CAAGGCTTTC 
TATGCTGCAG TGGCAGCAGA TTGTTTCAGG GATGGAGTTA GAAGCCTCCT TCAGGCCTCG 
GGCTTAGGAA GAATGAAACC AAACACTCTG GTGATTGGAT ATAAAAAAAA CTGGAGAAAA 
GCTCCCCTGA CAGAGATTGA AAACTACGTG GGAATCATAC ATGATGCATT TGATTTTGAG 
ATTGGTGTTG TCATAGTCAG AATCAGCCAA GGATTTGACA TCTCCCATGT TCTTCAGGTG 
CAAGATGAGT TGGAGAAATT AGAACAGGAG AGGCTGGCTT TGGAAGCCAC TATCAAAGAC 
AATGAATGTG AAGAAGGAAG TGGAGGCATC CGAGGCTTAT TTAAAAAAGC TGGCAAGCTG 
AACATTACTA AGACAACTCC TAAAAAAGAC AGCAGTATTA ACACAATCCA GTCGATGCAT 
GTGGGGGAGT TCAACCAGAA ACTGGTAGAA GCCAGCACCC AATTTAAAAA GAAGCAAGGA 
AAAGGCACAA TTGATGTTTG GTGGTTGTTT GATGATGGAG GGTTAACACT CCTTATCCCC 
TATATCTTGA CTCTCAGAAA AAAATGGAAA GACTGTAAAT TAAGAATCTA TGTTGGAGGG 
AAGATCAATC GCATTGAAGA AGAAAAAATT GCAATGGCTT CCCTTCTGAG CAAATTTAGG 
ATAAAATTTG CAGACATCCA TATCATTGGT GACATCAACA TTAAGCCAAA CAAAGAAAGT 
TGGAAAGTCT TTGAAGAGAT GATTGAACCA TATCGTCTCC ATGAAAGCTG CAAAGATTTA 
ACAACTGCTG AGAAATTAAA GAGAGAAACT CCGTGGAAAA TTACAGATGC AGAACTGGAA 
GCAGTCAAGG AAAAGAGTTA CCGCCAAGTT CGACTGAATG AACTCTTGCA AGAGCACTCA 
AGAGCTGCCA ATCTCATAGT TCTGAGCCTT CCAGTGGCAA GAAAAGGATC TATATCAGAT 
TGGTTGTACA TGGCTTGGTT GGAAATCCTC ACGAAGAACC TCCCTCCTGT CTTACTAGTT 
AGAGGAAATC ATAAAAATGT CCTGACATTT TATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840909.1
CDS	3468..6866
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_013985455.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985455.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTC TTGGAGTCAT CATCATTGGC CTGAGTGTGG TCGTGACAAC ACTCACAGGT 
ATTTCTATGT CTGCTATTTG CACAAATGGA GTAGTAAGAG GAGGTGGGGC TTACTACCTT 
ATTTCCAGAA GCTTAGGGCC CGAGTTTGGT GGGTCTATAG GCTTGATCTT TGCCTTTGCC 
AATGCAGTGG CTGTTGCTAT GTACGTGGTG GGATTTGCTG AGACTGTGGT AGATCTCCTT 
AAGGAGAGTG ATTCGATGAT GGTGGATCCA ACCAATGACA TCCGGATAAT AGGCTCCATC 
ACAGTGGTGA TTCTTTTAGG AATTTCAGTA GCTGGAATGG AATGGGAAGC GAAGGCTCAA 
GTGATTCTTC TGGTCATTCT TCTCATTGCT ATTGCAAACT TCTTCATTGG AACTGTCATT 
CCATCCAACA GTGAGAAAAA GTCCAGAGGT TTCTTTAATT ATCAAGCATC AATATTTGCA 
GAAAACTTTG GGCCAAGTTT CACAAAGGGT GAAGGCTTCT TCTCCGTCTT TGCCATTTTT 
TTCCCAGCAG CAACTGGGAT TCTAGCTGGT GCCAATATCT CAGGAGATTT AGAGGATCCC 
CAAGACGCCA TCCCCAAAGG GACGATGCTG GCCATCTTCA TCACTACTGT TGCCTACATA 
GGAGTTGCTA TTTGTGTAGG GGCCTGTGTG GTCAGAGATG CCACTGGAAG CATGAATGAC 
ACCATCATTT CTGGGATGAA TTGCAATGGT TCAGCAGCCT GTGGGTTAGG CTACGATTTC 
TCAAGATGTC GGCATGAACC ATGTCAATAT GGGCTGATGA ACAATTTCCA GGTCATGAGC 
ATGGTGTCAG GGTTTGGCCC CCTCATCACT GCTGGCATCT TTTCGGCGAC ACTCTCCTCG 
GCCCTGGCCT CCCTTGTCAG CGCACCCAAA GTGTTCCAGG CTCTGTGCAA GGACAACATC 
TACAAAGCCC TGCAGTTCTT TGCAAAGGGA TATGGGAAAA ACAATGAACC CTTGAGAGGA 
TACTTTCTCA CTTTTGTTAT AGCCATGGCG TTCATTCTTA TTGCGGAACT GAATACCATT 
GCTCCCATCA TTTCCAACTT TTTCCTCGCC TCATATGCAC TTATTAATTT CTCCTGCTTC 
CATGCTTCTT ATGCCAAATC TCCAGGATGG AGACCTGCAT ATGGTATTTA CAACATGTGG 
GTATCTCTTT TTGGAGCTAT TTTGTGCTGT GCAGTCATGT TTGTCATCAA CTGGTGGGCA 
GCTGTCATTA CCTATGTCAT TGAGTTCTTC CTCTATATCT ATGTGACTTA TAAGAAGCCA 
GATGTGAACT GGGGCTCCTC CACGCAGGCT CTTTCCTACG TGAGCGCTTT AGACAATGCT 
TTGGAACTAA CTACTGTGGA AGACCATGTG AAAAATTTCA GGCCCCAGTG CATTGTCTTA 
ACAGGGGGGC CCATGTCAAG ACCTGCTCTC TTGGATATAA CTCACGCCTT CACCAAGAAC 
AGTGGCCTTT GCATCTGCTG TGAAGTCTTT GTGGGACCGC GCAAGCTGTG TGTTAAAGAG 
ATGAACAGTG GCATGGCAAA AAAGCAGGCC TGGCTTATAA AGAACAAAAT CAAGGCTTTC 
TATGCTGCAG TGGCAGCAGA TTGTTTCAGG GATGGAGTTA GAAGCCTCCT TCAGGCCTCG 
GGCTTAGGAA GAATGAAACC AAACACTCTG GTGATTGGAT ATAAAAAAAA CTGGAGAAAA 
GCTCCCCTGA CAGAGATTGA AAACTACGTG GGAATCATAC ATGATGCATT TGATTTTGAG 
ATTGGTGTTG TCATAGTCAG AATCAGCCAA GGATTTGACA TCTCCCATGT TCTTCAGGTG 
CAAGATGAGT TGGAGAAATT AGAACAGGAG AGGCTGGCTT TGGAAGCCAC TATCAAAGAC 
AATGAATGTG AAGAAGGAAG TGGAGGCATC CGAGGCTTAT TTAAAAAAGC TGGCAAGCTG 
AACATTACTA AGACAACTCC TAAAAAAGAC AGCAGTATTA ACACAATCCA GTCGATGCAT 
GTGGGGGAGT TCAACCAGAA ACTGGTAGAA GCCAGCACCC AATTTAAAAA GAAGCAAGGA 
AAAGGCACAA TTGATGTTTG GTGGTTGTTT GATGATGGAG GGTTAACACT CCTTATCCCC 
TATATCTTGA CTCTCAGAAA AAAATGGAAA GACTGTAAAT TAAGAATCTA TGTTGGAGGG 
AAGATCAATC GCATTGAAGA AGAAAAAATT GCAATGGCTT CCCTTCTGAG CAAATTTAGG 
ATAAAATTTG CAGACATCCA TATCATTGGT GACATCAACA TTAAGCCAAA CAAAGAAAGT 
TGGAAAGTCT TTGAAGAGAT GATTGAACCA TATCGTCTCC ATGAAAGCTG CAAAGATTTA 
ACAACTGCTG AGAAATTAAA GAGAGAAACT CCGTGGAAAA TTACAGATGC AGAACTGGAA 
GCAGTCAAGG AAAAGAGTTA CCGCCAAGTT CGACTGAATG AACTCTTGCA AGAGCACTCA 
AGAGCTGCCA ATCTCATAGT TCTGAGCCTT CCAGTGGCAA GAAAAGGATC TATATCAGAT 
TGGTTGTACA TGGCTTGGTT GGAAATCCTC ACGAAGAACC TCCCTCCTGT CTTACTAGTT 
AGAGGAAATC ATAAAAATGT CCTGACATTT TATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_013985468.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840922.1 Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe31176
Clone ID Related Accession (Same CDS sequence)	XM_013985468.1
Accession Version	XM_013985468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	solute carrier family 12 member 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534009.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTC TTGGAGTCAT CATCATTGGC CTGAGTGTGG TCGTGACAAC ACTCACAGGT 
ATTTCTATGT CTGCTATTTG CACAAATGGA GTAGTAAGAG GAGGTGGGGC TTACTACCTT 
ATTTCCAGAA GCTTAGGGCC CGAGTTTGGT GGGTCTATAG GCTTGATCTT TGCCTTTGCC 
AATGCAGTGG CTGTTGCTAT GTACGTGGTG GGATTTGCTG AGACTGTGGT AGATCTCCTT 
AAGGAGAGTG ATTCGATGAT GGTGGATCCA ACCAATGACA TCCGGATAAT AGGCTCCATC 
ACAGTGGTGA TTCTTTTAGG AATTTCAGTA GCTGGAATGG AATGGGAAGC GAAGGCTCAA 
GTGATTCTTC TGGTCATTCT TCTCATTGCT ATTGCAAACT TCTTCATTGG AACTGTCATT 
CCATCCAACA GTGAGAAAAA GTCCAGAGGT TTCTTTAATT ATCAAGCATC AATATTTGCA 
GAAAACTTTG GGCCAAGTTT CACAAAGGGT GAAGGCTTCT TCTCCGTCTT TGCCATTTTT 
TTCCCAGCAG CAACTGGGAT TCTAGCTGGT GCCAATATCT CAGGAGATTT AGAGGATCCC 
CAAGACGCCA TCCCCAAAGG GACGATGCTG GCCATCTTCA TCACTACTGT TGCCTACATA 
GGAGTTGCTA TTTGTGTAGG GGCCTGTGTG GTCAGAGATG CCACTGGAAG CATGAATGAC 
ACCATCATTT CTGGGATGAA TTGCAATGGT TCAGCAGCCT GTGGGTTAGG CTACGATTTC 
TCAAGATGTC GGCATGAACC ATGTCAATAT GGGCTGATGA ACAATTTCCA GGTCATGAGC 
ATGGTGTCAG GGTTTGGCCC CCTCATCACT GCTGGCATCT TTTCGGCGAC ACTCTCCTCG 
GCCCTGGCCT CCCTTGTCAG CGCACCCAAA GTGTTCCAGG CTCTGTGCAA GGACAACATC 
TACAAAGCCC TGCAGTTCTT TGCAAAGGGA TATGGGAAAA ACAATGAACC CTTGAGAGGA 
TACTTTCTCA CTTTTGTTAT AGCCATGGCG TTCATTCTTA TTGCGGAACT GAATACCATT 
GCTCCCATCA TTTCCAACTT TTTCCTCGCC TCATATGCAC TTATTAATTT CTCCTGCTTC 
CATGCTTCTT ATGCCAAATC TCCAGGATGG AGACCTGCAT ATGGTATTTA CAACATGTGG 
GTATCTCTTT TTGGAGCTAT TTTGTGCTGT GCAGTCATGT TTGTCATCAA CTGGTGGGCA 
GCTGTCATTA CCTATGTCAT TGAGTTCTTC CTCTATATCT ATGTGACTTA TAAGAAGCCA 
GATGTGAACT GGGGCTCCTC CACGCAGGCT CTTTCCTACG TGAGCGCTTT AGACAATGCT 
TTGGAACTAA CTACTGTGGA AGACCATGTG AAAAATTTCA GGCCCCAGTG CATTGTCTTA 
ACAGGGGGGC CCATGTCAAG ACCTGCTCTC TTGGATATAA CTCACGCCTT CACCAAGAAC 
AGTGGCCTTT GCATCTGCTG TGAAGTCTTT GTGGGACCGC GCAAGCTGTG TGTTAAAGAG 
ATGAACAGTG GCATGGCAAA AAAGCAGGCC TGGCTTATAA AGAACAAAAT CAAGGCTTTC 
TATGCTGCAG TGGCAGCAGA TTGTTTCAGG GATGGAGTTA GAAGCCTCCT TCAGGCCTCG 
GGCTTAGGAA GAATGAAACC AAACACTCTG GTGATTGGAT ATAAAAAAAA CTGGAGAAAA 
GCTCCCCTGA CAGAGATTGA AAACTACGTG GGAATCATAC ATGATGCATT TGATTTTGAG 
ATTGGTGTTG TCATAGTCAG AATCAGCCAA GGATTTGACA TCTCCCATGT TCTTCAGGTG 
CAAGATGAGT TGGAGAAATT AGAACAGGAG AGGCTGGCTT TGGAAGCCAC TATCAAAGAC 
AATGAATGTG AAGAAGGAAG TGGAGGCATC CGAGGCTTAT TTAAAAAAGC TGGCAAGCTG 
AACATTACTA AGACAACTCC TAAAAAAGAC AGCAGTATTA ACACAATCCA GTCGATGCAT 
GTGGGGGAGT TCAACCAGAA ACTGGTAGAA GCCAGCACCC AATTTAAAAA GAAGCAAGGA 
AAAGGCACAA TTGATGTTTG GTGGTTGTTT GATGATGGAG GGTTAACACT CCTTATCCCC 
TATATCTTGA CTCTCAGAAA AAAATGGAAA GACTGTAAAT TAAGAATCTA TGTTGGAGGG 
AAGATCAATC GCATTGAAGA AGAAAAAATT GCGCTTTCAA CAAGCATTTG GCACAGAAGC 
CTCATCTCAA ATCTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840922.1
CDS	3469..6489
Translation	MSLNNSSNAFLDSGPGNTNRFQVNVISESHESSAAVNSNADPPHYEETSFGDESQNRFRISFRPGNQECYDNFLQNGEMAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVAPGSADVVANGDGMPGDERSENKEEEDQTGGVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNSEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMSRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISHVLQVQDELEKLEQERLALEATIKDNECEEGSGGIRGLFKKAGKLNITKTTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIALSTSIWHRSLISNLE

Target ORF information:

RefSeq Version	XM_013985468.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985468.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGCATTT CTGGATTCTG GGCCTGGTAA TACCAATCGC 
TTTCAAGTCA ATGTCATAAG TGAGAGCCAT GAGAGCAGTG CGGCTGTGAA TAGCAACGCT 
GACCCTCCAC ACTATGAGGA AACCTCTTTT GGGGATGAAT CCCAGAACAG ATTCAGAATC 
AGCTTTAGAC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAATGG AGAAATGGCT 
AAAACAGATG CCAGTTTTCA TGCTTATGAT TCTCACACAA ACACATACTA CCTACAAACT 
TTTGGCCACA ACACCGTGGA CGCTGTCCCC AAGATCGAGT ACTACCGCAA CACTGGCAGC 
ATCAGTGGAC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTTGCCAAG 
AATGTAGCAG TGGCCCCGGG TTCAGCTGAT GTGGTTGCTA ATGGAGATGG GATGCCTGGA 
GATGAACGAT CTGAAAACAA GGAAGAAGAA GATCAAACCG GTGGTGTGAA GTTTGGATGG 
GTGAAAGGTG TGCTGGTAAG ATGCATGCTG AACATCTGGG GTGTCATGCT CTTCATTCGC 
CTCTCTTGGA TTGTTGGAGA AGCTGGAATT GGTCTTGGAG TTCTCATAAT TCTTCTTTCC 
ACCATGGTAA CTTCTATTAC TGGGTTGTCA ACTTCTGCAA TAGCAACTAA CGGGTTTGTT 
CGTGGAGGTC TTGGAGTCAT CATCATTGGC CTGAGTGTGG TCGTGACAAC ACTCACAGGT 
ATTTCTATGT CTGCTATTTG CACAAATGGA GTAGTAAGAG GAGGTGGGGC TTACTACCTT 
ATTTCCAGAA GCTTAGGGCC CGAGTTTGGT GGGTCTATAG GCTTGATCTT TGCCTTTGCC 
AATGCAGTGG CTGTTGCTAT GTACGTGGTG GGATTTGCTG AGACTGTGGT AGATCTCCTT 
AAGGAGAGTG ATTCGATGAT GGTGGATCCA ACCAATGACA TCCGGATAAT AGGCTCCATC 
ACAGTGGTGA TTCTTTTAGG AATTTCAGTA GCTGGAATGG AATGGGAAGC GAAGGCTCAA 
GTGATTCTTC TGGTCATTCT TCTCATTGCT ATTGCAAACT TCTTCATTGG AACTGTCATT 
CCATCCAACA GTGAGAAAAA GTCCAGAGGT TTCTTTAATT ATCAAGCATC AATATTTGCA 
GAAAACTTTG GGCCAAGTTT CACAAAGGGT GAAGGCTTCT TCTCCGTCTT TGCCATTTTT 
TTCCCAGCAG CAACTGGGAT TCTAGCTGGT GCCAATATCT CAGGAGATTT AGAGGATCCC 
CAAGACGCCA TCCCCAAAGG GACGATGCTG GCCATCTTCA TCACTACTGT TGCCTACATA 
GGAGTTGCTA TTTGTGTAGG GGCCTGTGTG GTCAGAGATG CCACTGGAAG CATGAATGAC 
ACCATCATTT CTGGGATGAA TTGCAATGGT TCAGCAGCCT GTGGGTTAGG CTACGATTTC 
TCAAGATGTC GGCATGAACC ATGTCAATAT GGGCTGATGA ACAATTTCCA GGTCATGAGC 
ATGGTGTCAG GGTTTGGCCC CCTCATCACT GCTGGCATCT TTTCGGCGAC ACTCTCCTCG 
GCCCTGGCCT CCCTTGTCAG CGCACCCAAA GTGTTCCAGG CTCTGTGCAA GGACAACATC 
TACAAAGCCC TGCAGTTCTT TGCAAAGGGA TATGGGAAAA ACAATGAACC CTTGAGAGGA 
TACTTTCTCA CTTTTGTTAT AGCCATGGCG TTCATTCTTA TTGCGGAACT GAATACCATT 
GCTCCCATCA TTTCCAACTT TTTCCTCGCC TCATATGCAC TTATTAATTT CTCCTGCTTC 
CATGCTTCTT ATGCCAAATC TCCAGGATGG AGACCTGCAT ATGGTATTTA CAACATGTGG 
GTATCTCTTT TTGGAGCTAT TTTGTGCTGT GCAGTCATGT TTGTCATCAA CTGGTGGGCA 
GCTGTCATTA CCTATGTCAT TGAGTTCTTC CTCTATATCT ATGTGACTTA TAAGAAGCCA 
GATGTGAACT GGGGCTCCTC CACGCAGGCT CTTTCCTACG TGAGCGCTTT AGACAATGCT 
TTGGAACTAA CTACTGTGGA AGACCATGTG AAAAATTTCA GGCCCCAGTG CATTGTCTTA 
ACAGGGGGGC CCATGTCAAG ACCTGCTCTC TTGGATATAA CTCACGCCTT CACCAAGAAC 
AGTGGCCTTT GCATCTGCTG TGAAGTCTTT GTGGGACCGC GCAAGCTGTG TGTTAAAGAG 
ATGAACAGTG GCATGGCAAA AAAGCAGGCC TGGCTTATAA AGAACAAAAT CAAGGCTTTC 
TATGCTGCAG TGGCAGCAGA TTGTTTCAGG GATGGAGTTA GAAGCCTCCT TCAGGCCTCG 
GGCTTAGGAA GAATGAAACC AAACACTCTG GTGATTGGAT ATAAAAAAAA CTGGAGAAAA 
GCTCCCCTGA CAGAGATTGA AAACTACGTG GGAATCATAC ATGATGCATT TGATTTTGAG 
ATTGGTGTTG TCATAGTCAG AATCAGCCAA GGATTTGACA TCTCCCATGT TCTTCAGGTG 
CAAGATGAGT TGGAGAAATT AGAACAGGAG AGGCTGGCTT TGGAAGCCAC TATCAAAGAC 
AATGAATGTG AAGAAGGAAG TGGAGGCATC CGAGGCTTAT TTAAAAAAGC TGGCAAGCTG 
AACATTACTA AGACAACTCC TAAAAAAGAC AGCAGTATTA ACACAATCCA GTCGATGCAT 
GTGGGGGAGT TCAACCAGAA ACTGGTAGAA GCCAGCACCC AATTTAAAAA GAAGCAAGGA 
AAAGGCACAA TTGATGTTTG GTGGTTGTTT GATGATGGAG GGTTAACACT CCTTATCCCC 
TATATCTTGA CTCTCAGAAA AAAATGGAAA GACTGTAAAT TAAGAATCTA TGTTGGAGGG 
AAGATCAATC GCATTGAAGA AGAAAAAATT GCGCTTTCAA CAAGCATTTG GCACAGAAGC 
CTCATCTCAA ATCTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SLC12A1 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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