MAP3K20 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K20
Entrez Gene ID	100152572
Full Name	mitogen-activated protein kinase kinase kinase 20
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 15 Map Location: 15\|15

mRNA and Protein(s)

mRNA	Protein	Name
XM_013990583.1	XP_013846037.1	LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase kinase MLT
XM_013990583.2	XP_013846037.2	mitogen-activated protein kinase kinase kinase MLT isoform X1
XM_021075200.1	XP_020930859.1	mitogen-activated protein kinase kinase kinase MLT isoform X2
XM_021075201.1	XP_020930860.1	mitogen-activated protein kinase kinase kinase MLT isoform X2
XM_021075198.1	XP_020930857.1	mitogen-activated protein kinase kinase kinase MLT isoform X1
XM_021075199.1	XP_020930858.1	mitogen-activated protein kinase kinase kinase MLT isoform X1
XM_021075202.1	XP_020930861.1	mitogen-activated protein kinase kinase kinase MLT isoform X2

Pathways from BioSystems
Gene Ontology

The following MAP3K20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe239466	XM_021075202.1 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OSe239466	XM_021075200.1 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OSe239466	XM_021075201.1 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OSe23541	XM_013990583.1 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase MLT (LOC100152572), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe239465	XM_013990583.2 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe239465	XM_021075199.1 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe239465	XM_021075198.1 Latest version!	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K20 ( XM_021075200.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930859.1 Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe239466
Clone ID Related Accession (Same CDS sequence)	XM_021075200.1 , XM_021075201.1 , XM_021075202.1
Accession Version	XM_021075200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA GACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACGA GTAACGGGGA GGGCCATGGC ATGAACCAAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAGGTCAACA TGGCCCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGACGAT 
GACGATGATG AGGATGAGGA TGGTGATGAG GATAATGACG TGGATAATAA TAGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930859.1
CDS	348..1727
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNQSLQAMMLMGFGDIFSMNKAGAVLHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDEDEDGDEDNDVDNNSE

Target ORF information:

RefSeq Version	XM_021075200.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021075200.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA GACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACGA GTAACGGGGA GGGCCATGGC ATGAACCAAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAGGTCAACA TGGCCCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGACGAT 
GACGATGATG AGGATGAGGA TGGTGATGAG GATAATGACG TGGATAATAA TAGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_021075201.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930860.1 Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe239466
Clone ID Related Accession (Same CDS sequence)	XM_021075200.1 , XM_021075201.1 , XM_021075202.1
Accession Version	XM_021075201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA GACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACGA GTAACGGGGA GGGCCATGGC ATGAACCAAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAGGTCAACA TGGCCCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGACGAT 
GACGATGATG AGGATGAGGA TGGTGATGAG GATAATGACG TGGATAATAA TAGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930860.1
CDS	399..1778
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNQSLQAMMLMGFGDIFSMNKAGAVLHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDEDEDGDEDNDVDNNSE

Target ORF information:

RefSeq Version	XM_021075201.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021075201.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA GACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACGA GTAACGGGGA GGGCCATGGC ATGAACCAAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAGGTCAACA TGGCCCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGACGAT 
GACGATGATG AGGATGAGGA TGGTGATGAG GATAATGACG TGGATAATAA TAGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_021075202.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930861.1 Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSe239466
Clone ID Related Accession (Same CDS sequence)	XM_021075200.1 , XM_021075201.1 , XM_021075202.1
Accession Version	XM_021075202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA GACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACGA GTAACGGGGA GGGCCATGGC ATGAACCAAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAGGTCAACA TGGCCCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGACGAT 
GACGATGATG AGGATGAGGA TGGTGATGAG GATAATGACG TGGATAATAA TAGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930861.1
CDS	321..1700
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNQSLQAMMLMGFGDIFSMNKAGAVLHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDEDEDGDEDNDVDNNSE

Target ORF information:

RefSeq Version	XM_021075202.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021075202.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA GACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACGA GTAACGGGGA GGGCCATGGC ATGAACCAAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAGGTCAACA TGGCCCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGACGAT 
GACGATGATG AGGATGAGGA TGGTGATGAG GATAATGACG TGGATAATAA TAGTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_013990583.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013846037.1 Sus scrofa mitogen-activated protein kinase kinase kinase MLT (LOC100152572), mRNA.

Price & Availability↑

CloneID	OSe23541
Clone ID Related Accession (Same CDS sequence)	XM_013990583.1
Accession Version	XM_013990583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase kinase MLT
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003536441.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGCGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCAAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTAAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TGGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ACCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCAGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
GACCATCCCG GATGCCCCCT GCGCTTCGAC ACCGAGTGGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013846037.1
CDS	347..2746
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHLKSAIEKLTHDYLNLFHFPPLIKDSVGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIIGILENQTVECTVTYESDVRTPKSTKHVHSIKWSKTKPQDEVKAVQLAIQTSFSNSEGNPRSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSQFFPYFHDPDSYAAAVRRTQVPIKYQQITPINQSRSSSPTQYALTKHFSSLHLNSKDSSFSSLNSNSLERGRYSGRSRNKYDRGSVSLNSSPRGRYGGKSQHSTPSRERYSGKFYRVSQSALNTHSSPDLKRSPNDSQQPRDHPGCPLRFDTEWRASEEESKVSEGGWTRVEYRKKPHRPSPAKTNRERPRGTYRGKRNF

Target ORF information:

RefSeq Version	XM_013990583.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase MLT (LOC100152572), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013990583.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGCGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCAAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTAAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TGGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ACCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCAGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
GACCATCCCG GATGCCCCCT GCGCTTCGAC ACCGAGTGGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_013990583.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013846037.2 Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe239465
Clone ID Related Accession (Same CDS sequence)	XM_013990583.2 , XM_021075198.1 , XM_021075199.1
Accession Version	XM_013990583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013990583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCGAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTGAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TAGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ATCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCGGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
ACCATCCCGG GGATGCCCCT GCGCTTCGAC ACCGAGTCGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013846037.2
CDS	279..2678
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHLKSAIEKLTHDYLNLFHFPPLIKDSVGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIIGILENQTVECTVTYESDVRTPKSTKHVHSIKWSKTKPQDEVKAVQLAIQTSFSNSEGNPRSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSQFFPYFHDPDSYAAAVRRTQVPIKYQQITPINQSRSSSPTQYALTKHFSSLHLNSKDSSFSSLNSNSLERGRYSGRSRNKYDRGSVSLNSSPRGRYGGKSQHSTPSRERYSGKFYRVSQSALNTHSSPDLKRSPNDSQQPRTIPGMPLRFDTESRASEEESKVSEGGWTRVEYRKKPHRPSPAKTNRERPRGTYRGKRNF

Target ORF information:

RefSeq Version	XM_013990583.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013990583.2

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCGAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTGAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TAGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ATCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCGGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
ACCATCCCGG GGATGCCCCT GCGCTTCGAC ACCGAGTCGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_021075198.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930857.1 Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe239465
Clone ID Related Accession (Same CDS sequence)	XM_013990583.2 , XM_021075198.1 , XM_021075199.1
Accession Version	XM_021075198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCGAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTGAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TAGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ATCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCGGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
ACCATCCCGG GGATGCCCCT GCGCTTCGAC ACCGAGTCGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930857.1
CDS	441..2840
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHLKSAIEKLTHDYLNLFHFPPLIKDSVGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIIGILENQTVECTVTYESDVRTPKSTKHVHSIKWSKTKPQDEVKAVQLAIQTSFSNSEGNPRSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSQFFPYFHDPDSYAAAVRRTQVPIKYQQITPINQSRSSSPTQYALTKHFSSLHLNSKDSSFSSLNSNSLERGRYSGRSRNKYDRGSVSLNSSPRGRYGGKSQHSTPSRERYSGKFYRVSQSALNTHSSPDLKRSPNDSQQPRTIPGMPLRFDTESRASEEESKVSEGGWTRVEYRKKPHRPSPAKTNRERPRGTYRGKRNF

Target ORF information:

RefSeq Version	XM_021075198.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021075198.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCGAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTGAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TAGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ATCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCGGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
ACCATCCCGG GGATGCCCCT GCGCTTCGAC ACCGAGTCGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_021075199.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020930858.1 Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X3, mRNA.

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CloneID	OSe239465
Clone ID Related Accession (Same CDS sequence)	XM_013990583.2 , XM_021075198.1 , XM_021075199.1
Accession Version	XM_021075199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	mitogen-activated protein kinase kinase kinase MLT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010457.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCGAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTGAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TAGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ATCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCGGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
ACCATCCCGG GGATGCCCCT GCGCTTCGAC ACCGAGTCGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020930858.1
CDS	355..2754
Translation	MSSLGTSFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADPKKRPSFKQIISILESMSNDTNLPDQCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHLKSAIEKLTHDYLNLFHFPPLIKDSVGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIIGILENQTVECTVTYESDVRTPKSTKHVHSIKWSKTKPQDEVKAVQLAIQTSFSNSEGNPRSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSQFFPYFHDPDSYAAAVRRTQVPIKYQQITPINQSRSSSPTQYALTKHFSSLHLNSKDSSFSSLNSNSLERGRYSGRSRNKYDRGSVSLNSSPRGRYGGKSQHSTPSRERYSGKFYRVSQSALNTHSSPDLKRSPNDSQQPRTIPGMPLRFDTESRASEEESKVSEGGWTRVEYRKKPHRPSPAKTNRERPRGTYRGKRNF

Target ORF information:

RefSeq Version	XM_021075199.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021075199.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTACCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTCGAA 
AACTGCGGCG GAGGAAGTTT TGGGAGTGTC TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TGAAGAAGCT ACTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGTATC 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAA 
GAGATGGACA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCACTAT 
TTACACATGG AGGCTCCTGT CAAGGTGATT CACAGAGATC TCAAGTCAAG AAATGTTGTT 
ATAGCTGCTG ATGGGGTGTT GAAGATCTGC GACTTTGGCG CCTCTCGGTT CCACAACCAT 
ACAACACACA TGTCCTTGGT GGGAACTTTC CCATGGATGG CACCAGAAGT TATCCAAAGT 
CTCCCTGTGT CTGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGGA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATCC CAAGAAACGG CCATCTTTCA AGCAGATCAT TTCAATTCTG 
GAGTCCATGT CGAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTCCT GCACAACAAG 
GCAGAGTGGA GGTGTGAAAT CGAAGCAACT CTTGAGAGGC TGAAGAAACT AGAACGTGAC 
CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGAGAAAGGC GTCTGAAGAT GTGGGAGCAA 
AAGCTGACGG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCTTGGACG 
GAAGATGACG TGTATTTTTG GGTTCAGCAA CTCGTCAGAA AAGGTGACTC GTCAGCAGAG 
ATGAGTGTGT ATGCGAGCTT GTTTAAGGAA AACAACATAA CGGGAAAGCG ACTGCTGCTT 
CTCGAGGAAG AAGACTTGAA AGACATGGGC ATCGTCTCCA AGGGGCATAT CATCCATTTA 
AAGTCAGCCA TTGAGAAATT AACCCACGAT TACCTAAACT TGTTTCACTT CCCACCACTT 
ATTAAGGACT CAGTAGGAGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTTGAACTT 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACGTACATCA AAGATGTAAC ATTCAACACT 
AACCTGCCTG ACGCAGAGAT TTTAAAGATG ACAAAGCCCC CGTTTGTGAT GGAGAAGTGG 
ATTATAGGAA TACTGGAAAA TCAGACTGTA GAATGCACTG TCACATACGA GAGCGACGTT 
AGAACTCCAA AAAGCACTAA ACACGTCCAT TCAATTAAGT GGAGTAAAAC GAAACCTCAG 
GATGAAGTGA AAGCCGTCCA GCTTGCCATT CAGACGTCAT TCTCCAACTC AGAGGGCAAC 
CCCAGAAGCA GGTCTGACTC AAGTGCCGAC TGCCAGTGGT TAGACACTCT GAGGATGCGG 
CAGATTGCGT CCAACACGTC TTTACAGCGC TCCCAGAGCA ATCCCATCCT GGGGTCACAA 
TTCTTTCCCT ACTTCCATGA CCCGGACTCC TATGCTGCTG CTGTGAGGCG GACTCAGGTG 
CCCATTAAGT ATCAACAGAT AACACCTATA AACCAGTCCA GAAGCTCCTC TCCCACTCAG 
TACGCACTGA CCAAACACTT CTCTTCTCTG CACCTCAACT CCAAGGACAG CAGCTTTTCC 
AGTCTGAATT CTAACTCTTT GGAGAGGGGG CGATATTCAG GTAGAAGCAG GAACAAATAT 
GATCGGGGTA GCGTATCACT CAATTCTTCT CCCAGAGGAA GATACGGGGG GAAAAGTCAG 
CATTCCACGC CTTCAAGAGA AAGATATTCT GGAAAATTCT ACAGGGTGTC TCAGTCAGCC 
CTCAACACTC ACTCGTCGCC CGACCTCAAG AGGAGCCCCA ATGACTCCCA GCAGCCCAGG 
ACCATCCCGG GGATGCCCCT GCGCTTCGAC ACCGAGTCGA GAGCCAGTGA GGAGGAGAGC 
AAAGTCAGCG AAGGGGGCTG GACGCGGGTG GAATACCGCA AGAAACCCCA CAGGCCCTCT 
CCGGCCAAGA CCAACCGAGA AAGACCCAGG GGAACCTACC GTGGGAAGAG AAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAP3K20 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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