DBT cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	DBT
Entrez Gene ID	104151276
Full Name	dihydrolipoamide branched chain transacylase E2
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009686022.1	XP_009684317.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following DBT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt81316	XM_009686022.1 Latest version!	Struthio camelus australis dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBT ( XM_009686022.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009684317.1 Struthio camelus australis dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.

Price & Availability↑

CloneID	OSt81316
Clone ID Related Accession (Same CDS sequence)	XM_009686022.1
Accession Version	XM_009686022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009272064.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGCTTAGCA CCTCGAAACA TGTTTCTGAG ACGCTCTGCA AAGCGGTGCT ACCTGCAGGG 
CGGAGGCGAG AGGCGGCCCC CTCGCCCCGG CTGAGCAGAG GCAGGAAAGG GCGAAAGAGA 
AGAGGGGCCG AGGTCTGCAA GCAGGCCTCA GTTGAGACAC CTCACCACCC TTCAAGAGGT 
GTCCTGTTTT CTGGCTCTTC TAAGAAGATA ATTTGTAAAT TTTACAGTTT TGAGCTACTT 
GAGGTTTGTG TTCACCGTGT TAGATCGTGC AGCAGCATTC GGTTTATAAA ATCAAAATAT 
GTGTGCGTGT TTGACAAATC TGCCCTCAAG TTTAGTCATC AACAGCGATT ATTCAGAGCA 
TCCGCTGTTT CATGTGGCCA AATTGTGCAG TTTAAGCTTT CTGATATTGG AGAAGGGATT 
ACAGAGGTGA CTGTGAAAGA ATGGTATATA AAAGAAGGTG ATAGTGTGTC TCAGTTTGAT 
AGCATCTGTG AAGTACAAAG CGATAAAGCT TCTGTTACTA TTACTAGCCG TTATGATGGC 
ATCATTAGAA AACTCCATTA CAATTTAGAT GAAATTGCTT ATGTTGGAAA ACCATTAGTG 
GACATTGAAA TTGATGCTTC AAAAGGTGTT GCCCCAGAAG AAGATGTTGT TGAAACACCT 
GCTATGTCTC ATGAAGAACA CACTCACCAA GAGATAAAAG GTCATAAAAC ATTGGCAACC 
CCTGCAGTTC GTCGTCTTGC CATGGAAAAC AATATTAAAT TGAGTGAAGT TGTTGGGACA 
GGGAAGGATA ACAGAATCCT TAAGGAAGAC ATACTCAATT ATTTGGCCAA ACAGACAGGA 
GCTATTTTAC CTCCATCACC AAAAGCTGAA ATTCTCCCAC CTTTGCCAAA ATCCGAGACA 
GTGCCAGCTG CTCCAAAGGA TAAAGCACGT AAAATTCCTG TACCAGTTTC CAGACCCATT 
GCGTTTTCAG GAAAAGATAA AACTGAAGCT ATAACAGGTT TTCACAAGGC AATGGTAAAG 
ACTATGAGTG CAGCCTTAAA GATCCCTCAC TTTGGTTATT GCGATGAGAT TGATTTGACT 
CAGCTCGTTC AGCTGCGAGA AGAGCTGAAA CCTCTAGCAC AAACTCGTGG AGTTAAGCTT 
TCCTTTATGC CCTTCTTCAT AAAGGCAGCT TCTCTGGGAT TGTTGCAGTA TCCTATTCTT 
AATGCTTCTT TGGATGAAAG CTGTCAAAAC ATCACATACA AGGCTTCGCA CAACATTGGA 
GTTGCTATGG ACACAGAGCA AGGTTTAATT GTCCCAAATG TGAAAAATGT TCAAGTCTGT 
AGTGTATTTG AGATTGCTTC TGAATTAAAT CGTCTACAAT CCTTGGGCTC TGCAGGCCAG 
CTGGGAACAA ATGACCTCAC TGGGGGAACA TTCACCCTTT CAAATATTGG CACAATTGGT 
GGCACTTATA CTAAACCAAT GATACTACCT CCTGAAGTAG CTATTGGAGC CCTTGGAAAG 
ATACAGGTTC TTCCCCGGTT TAATGGAAAA GGTGAAGTAT TTAAAGCACA GATAATGAAT 
GTGAGTTGGT CAGCTGATCA CCGCATTATT GATGGAGCTA CAATGGCCCG GTTTTCTAAC 
TTGTGGAAAT CTTATTTGGA GAACCCTGCT TTAATGCTGC TAGACCTTAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009684317.1
CDS	1..1674
Translation	MLSTSKHVSETLCKAVLPAGRRREAAPSPRLSRGRKGRKRRGAEVCKQASVETPHHPSRGVLFSGSSKKIICKFYSFELLEVCVHRVRSCSSIRFIKSKYVCVFDKSALKFSHQQRLFRASAVSCGQIVQFKLSDIGEGITEVTVKEWYIKEGDSVSQFDSICEVQSDKASVTITSRYDGIIRKLHYNLDEIAYVGKPLVDIEIDASKGVAPEEDVVETPAMSHEEHTHQEIKGHKTLATPAVRRLAMENNIKLSEVVGTGKDNRILKEDILNYLAKQTGAILPPSPKAEILPPLPKSETVPAAPKDKARKIPVPVSRPIAFSGKDKTEAITGFHKAMVKTMSAALKIPHFGYCDEIDLTQLVQLREELKPLAQTRGVKLSFMPFFIKAASLGLLQYPILNASLDESCQNITYKASHNIGVAMDTEQGLIVPNVKNVQVCSVFEIASELNRLQSLGSAGQLGTNDLTGGTFTLSNIGTIGGTYTKPMILPPEVAIGALGKIQVLPRFNGKGEVFKAQIMNVSWSADHRIIDGATMARFSNLWKSYLENPALMLLDLK

Target ORF information:

RefSeq Version	XM_009686022.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009686022.1

ORF Insert Sequence:

ATGCTTAGCA CCTCGAAACA TGTTTCTGAG ACGCTCTGCA AAGCGGTGCT ACCTGCAGGG 
CGGAGGCGAG AGGCGGCCCC CTCGCCCCGG CTGAGCAGAG GCAGGAAAGG GCGAAAGAGA 
AGAGGGGCCG AGGTCTGCAA GCAGGCCTCA GTTGAGACAC CTCACCACCC TTCAAGAGGT 
GTCCTGTTTT CTGGCTCTTC TAAGAAGATA ATTTGTAAAT TTTACAGTTT TGAGCTACTT 
GAGGTTTGTG TTCACCGTGT TAGATCGTGC AGCAGCATTC GGTTTATAAA ATCAAAATAT 
GTGTGCGTGT TTGACAAATC TGCCCTCAAG TTTAGTCATC AACAGCGATT ATTCAGAGCA 
TCCGCTGTTT CATGTGGCCA AATTGTGCAG TTTAAGCTTT CTGATATTGG AGAAGGGATT 
ACAGAGGTGA CTGTGAAAGA ATGGTATATA AAAGAAGGTG ATAGTGTGTC TCAGTTTGAT 
AGCATCTGTG AAGTACAAAG CGATAAAGCT TCTGTTACTA TTACTAGCCG TTATGATGGC 
ATCATTAGAA AACTCCATTA CAATTTAGAT GAAATTGCTT ATGTTGGAAA ACCATTAGTG 
GACATTGAAA TTGATGCTTC AAAAGGTGTT GCCCCAGAAG AAGATGTTGT TGAAACACCT 
GCTATGTCTC ATGAAGAACA CACTCACCAA GAGATAAAAG GTCATAAAAC ATTGGCAACC 
CCTGCAGTTC GTCGTCTTGC CATGGAAAAC AATATTAAAT TGAGTGAAGT TGTTGGGACA 
GGGAAGGATA ACAGAATCCT TAAGGAAGAC ATACTCAATT ATTTGGCCAA ACAGACAGGA 
GCTATTTTAC CTCCATCACC AAAAGCTGAA ATTCTCCCAC CTTTGCCAAA ATCCGAGACA 
GTGCCAGCTG CTCCAAAGGA TAAAGCACGT AAAATTCCTG TACCAGTTTC CAGACCCATT 
GCGTTTTCAG GAAAAGATAA AACTGAAGCT ATAACAGGTT TTCACAAGGC AATGGTAAAG 
ACTATGAGTG CAGCCTTAAA GATCCCTCAC TTTGGTTATT GCGATGAGAT TGATTTGACT 
CAGCTCGTTC AGCTGCGAGA AGAGCTGAAA CCTCTAGCAC AAACTCGTGG AGTTAAGCTT 
TCCTTTATGC CCTTCTTCAT AAAGGCAGCT TCTCTGGGAT TGTTGCAGTA TCCTATTCTT 
AATGCTTCTT TGGATGAAAG CTGTCAAAAC ATCACATACA AGGCTTCGCA CAACATTGGA 
GTTGCTATGG ACACAGAGCA AGGTTTAATT GTCCCAAATG TGAAAAATGT TCAAGTCTGT 
AGTGTATTTG AGATTGCTTC TGAATTAAAT CGTCTACAAT CCTTGGGCTC TGCAGGCCAG 
CTGGGAACAA ATGACCTCAC TGGGGGAACA TTCACCCTTT CAAATATTGG CACAATTGGT 
GGCACTTATA CTAAACCAAT GATACTACCT CCTGAAGTAG CTATTGGAGC CCTTGGAAAG 
ATACAGGTTC TTCCCCGGTT TAATGGAAAA GGTGAAGTAT TTAAAGCACA GATAATGAAT 
GTGAGTTGGT CAGCTGATCA CCGCATTATT GATGGAGCTA CAATGGCCCG GTTTTCTAAC 
TTGTGGAAAT CTTATTTGGA GAACCCTGCT TTAATGCTGC TAGACCTTAA ATAA