LOC590546 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC590546
Entrez Gene ID	590546
Full Name	epithelial chloride channel protein-like
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011664711.1	XP_011663013.1	epithelial chloride channel protein-like
XM_030979099.1	XP_030834959.1	calcium-activated chloride channel regulator 4A-like

Pathways from BioSystems
Gene Ontology

The following LOC590546 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC590546 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd45384	XM_030979099.1 Latest version!	Strongylocentrotus purpuratus calcium-activated chloride channel regulator 4A-like (LOC590546), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSd26650	XM_011664711.1 Latest version!	Strongylocentrotus purpuratus epithelial chloride channel protein-like (LOC590546), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC590546 ( XM_030979099.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030834959.1 Strongylocentrotus purpuratus calcium-activated chloride channel regulator 4A-like (LOC590546), mRNA.

Price & Availability↑

CloneID	OSd45384
Clone ID Related Accession (Same CDS sequence)	XM_030979099.1
Accession Version	XM_030979099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	calcium-activated chloride channel regulator 4A-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145595.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTCG AACGCCTCAG TTTGCTGCTT TTTTTCTCAG TCGGCCTGAT GGGAACCAAG 
ATCTGCAAGG CCCAACAGGA TCGTAATCCA ATCGAGCTAG AGAATGGAGC TTATAACAAC 
ATACTCATCG CTATAAGCAA AGATGTCGAA GAAGATTCAA GTATCATACA AAATATAAAG 
GATATCTTCA GGGCCGGATC TTCTATCCTT TTCAACGCTA CCAATCTGCG GGTCTACCTT 
GGGACTATCA CGATCCTAGT CCCGCCAACT TGGTCCAGAA ACTCTGAGTA TCAAGTTGCC 
CAACGTGAAG CCTATGAGAA TGCTAACGTT CTCGTTACTG ATCAACAGTC TAATCATCGT 
CCGTTTGTCG ATAAACCATT CAAATGTGGA CAACAAGGAA GATTCATGCA TTTATCCAAG 
ACTTTCCTCG TCGACCAAGC TTTACGCGAA AATCAATTTG GAGATTCTGG TAAAGTGATT 
GTCCGTCAGT TTGCCAAACT CCGTTGGGGC GTGTTTGACG AGGATTACGT GCCAGGGACC 
GGTGCTGAAC CTTACTACCA ATTAAACTCG AACACCGTAA CAGGTGGCGT CTTCGAAGGA 
ACACGATGCT CTTCGAAAGT TCTAGGAGAT CTCCTTGATG AAAATGAAAG GCAATGTCGG 
CAAGATCCTT CTGGGTATTT ACCCGACTCT TGCAGATTCG TGCCCCATGC CAGTGGGCAA 
ACTGCCAGGG CATCGTTAAT GTTTGCTTCC AACATCCACT CGATTGACAT GTTCTGCCAC 
GACGATCCAG GAAATGTGGG GTATCATAAT TACATGGCTC CTAACCTACA GAATAAGAAG 
TGCGACTATC AAAGCGTTTG GCAGCTGATG CGGAAATCGA CTGATTTCAT GAATGGAAAC 
AGTCCTTCCT TGCCAGAGGA TACCGACACA TCACCAAACT TTATTGTCGT GCAGCCGGCC 
GGATCTCTGA GGATTGTTCT TGTTTTAGAT ACCAGTTTTT CCATGGATGG GCCCCGTTTT 
AACAAGATGA TCAGAGGGGC CAAGAACTTC ATCCAGAGTA TAGTACCTAA TAATAGTTAC 
GTGGCCATTG TCGAGTTCAA TTCCGTATCC AAAGTGAATT CTAACATGAT TGAACTCAGC 
AGTGTCATAT CAAGGAAAAA CTTGGCGTCT CTACTTCCAA CGGTTGCCGA AAACAATACG 
TGTATTGGCT GCGGCATTTT GACAGCTATC GAGGTTGCCC AATACAACGG ATTGGATTCC 
CGAGGTGTGT ATCTCATTCT TCTCAGCGAC GGACAGGAGA ATATTTATGA AGACATAACT 
CTAGTAGCTG ACACTCTGGA TGACATCGAA AGTTCAGGTG TTACTGTGCA TTCAATCGCC 
TTTTACGGAG CCGACACCCA GTTGGAAGAG CTAGCCCAGA TGACAGGCGG TAGATCGGCA 
ACGTGTGCTG ACGGAGGGAG CTCACAATGC GTCATATCCG CCTTTGTGTC AATCATAGCG 
CAAAGTCCAC AGAGTGTTGC GACTTCTGCA CCAATACAGG TTCAGAGTTC AACGATCACA 
TTAGACCCTG GCACTACCAA GACTACCGAT ATCGTCATTG ATGATTCTCT AGGACTGGAT 
ACTGTCATAA CAATTACATG GACGGCCAAC GACATCATCA CTGTCACAGT CAGAGGACCA 
GATGGTACCG TTATAGACAG TACTGATGCA AGATATGAGA CTGATATCAG CTCTAAAATT 
ATTACCGTCA CCATCATAGA TGCAGAGGCA GGTGTATGGA GCATTGAAAT CAATAACGAC 
GGCAACCCTC ACATGGAATC CGTTAGTGTG GACGTGATTT CTAAACCTCG TTCTGACGGT 
GTTTACCCTC CAGTTGTAAA TAGCTTCTTA GGGACATCGA TAGTATACTA CACCACTGCT 
CCCCTGTTAG AGGTCTATGC ATATGTCCAT ATCAACTACG TACCCGTCAT CGACGCTACA 
GTGATTGCTG AAGTAGTCAG CTCGACAACA GGGGATGTGA CAACCATCTC GCTACTTGAT 
AACGGACTAG GAGCTGATTT GATCGCAGGT GATGGTATTT ACTCGGCTTA TTTCCTTGAT 
TTCTCATCGA ATGGACGATA TGGCGTCAAG GTAGATGTAA ACGGATACAC CGCCAATTCG 
TCAGAAAGTG CGCTGCGATC TAAACGAGAT ACGGGAACTC TGACCACTCC TGTCCCGTCT 
CTACGGTTCC AGCGAACGAC TACAGCGGGC GTCTTTCAAG TAGAAAACTA CTCTCCTGAT 
CTGATTTTTG ACATCCTCGC ACCATCTAAG ATCACTGATA TCACTAGCAG AGAGAGAACA 
TACGATGAGA ATAGAAATGT GACGTTGACA TGGTCAGCTG TAGGGGATGA CTTAGACCAA 
GGAACAGCTG CCTACTACGA GCTCCGTTTC TCAGATAACT TCACCCAAAT CCGAACCAAC 
TTTACCTCCG CTGCTGCTGT TAACGATACC GATCTCATAC TTGGTAACCT CTCCCGAGTC 
GCTTCATCTG GTACTCTCGA GTCCATCACT ATTCTTCTGC CTGGTCAGGA CTCAGACGCT 
ATCTTTTCAT TCATGATAAG AGCCTGGGAT GAGGCTGGCA ACGCAGGTCC TTTATCGAAC 
ATCGTATCTG TCTCAAGACG TTCCCTACCC CCGACTACCA TGCCGACTAC CATTCTGAAC 
TCGACATCGG AACCACCATC CACACAATCA CCTGGAGTAG CACTTCCAAT ATGGGTTGTC 
ATTTCATTTT CCTGCGCCGC ATTCTTCATC GTTATCGCTG TTTCCATTAT TGTTGGTGTG 
CTTTGTAATC GTAGCGCACC TAAGAATCCA ATAGGGTCGG TTTATGAGCA CACTAATCCT 
GCTTATGAAT GTTATGAACC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030834959.1
CDS	216..3119
Translation	MLVERLSLLLFFSVGLMGTKICKAQQDRNPIELENGAYNNILIAISKDVEEDSSIIQNIKDIFRAGSSILFNATNLRVYLGTITILVPPTWSRNSEYQVAQREAYENANVLVTDQQSNHRPFVDKPFKCGQQGRFMHLSKTFLVDQALRENQFGDSGKVIVRQFAKLRWGVFDEDYVPGTGAEPYYQLNSNTVTGGVFEGTRCSSKVLGDLLDENERQCRQDPSGYLPDSCRFVPHASGQTARASLMFASNIHSIDMFCHDDPGNVGYHNYMAPNLQNKKCDYQSVWQLMRKSTDFMNGNSPSLPEDTDTSPNFIVVQPAGSLRIVLVLDTSFSMDGPRFNKMIRGAKNFIQSIVPNNSYVAIVEFNSVSKVNSNMIELSSVISRKNLASLLPTVAENNTCIGCGILTAIEVAQYNGLDSRGVYLILLSDGQENIYEDITLVADTLDDIESSGVTVHSIAFYGADTQLEELAQMTGGRSATCADGGSSQCVISAFVSIIAQSPQSVATSAPIQVQSSTITLDPGTTKTTDIVIDDSLGLDTVITITWTANDIITVTVRGPDGTVIDSTDARYETDISSKIITVTIIDAEAGVWSIEINNDGNPHMESVSVDVISKPRSDGVYPPVVNSFLGTSIVYYTTAPLLEVYAYVHINYVPVIDATVIAEVVSSTTGDVTTISLLDNGLGADLIAGDGIYSAYFLDFSSNGRYGVKVDVNGYTANSSESALRSKRDTGTLTTPVPSLRFQRTTTAGVFQVENYSPDLIFDILAPSKITDITSRERTYDENRNVTLTWSAVGDDLDQGTAAYYELRFSDNFTQIRTNFTSAAAVNDTDLILGNLSRVASSGTLESITILLPGQDSDAIFSFMIRAWDEAGNAGPLSNIVSVSRRSLPPTTMPTTILNSTSEPPSTQSPGVALPIWVVISFSCAAFFIVIAVSIIVGVLCNRSAPKNPIGSVYEHTNPAYECYEP

Target ORF information:

RefSeq Version	XM_030979099.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus calcium-activated chloride channel regulator 4A-like (LOC590546), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030979099.1

ORF Insert Sequence:

ATGCTGGTCG AACGCCTCAG TTTGCTGCTT TTTTTCTCAG TCGGCCTGAT GGGAACCAAG 
ATCTGCAAGG CCCAACAGGA TCGTAATCCA ATCGAGCTAG AGAATGGAGC TTATAACAAC 
ATACTCATCG CTATAAGCAA AGATGTCGAA GAAGATTCAA GTATCATACA AAATATAAAG 
GATATCTTCA GGGCCGGATC TTCTATCCTT TTCAACGCTA CCAATCTGCG GGTCTACCTT 
GGGACTATCA CGATCCTAGT CCCGCCAACT TGGTCCAGAA ACTCTGAGTA TCAAGTTGCC 
CAACGTGAAG CCTATGAGAA TGCTAACGTT CTCGTTACTG ATCAACAGTC TAATCATCGT 
CCGTTTGTCG ATAAACCATT CAAATGTGGA CAACAAGGAA GATTCATGCA TTTATCCAAG 
ACTTTCCTCG TCGACCAAGC TTTACGCGAA AATCAATTTG GAGATTCTGG TAAAGTGATT 
GTCCGTCAGT TTGCCAAACT CCGTTGGGGC GTGTTTGACG AGGATTACGT GCCAGGGACC 
GGTGCTGAAC CTTACTACCA ATTAAACTCG AACACCGTAA CAGGTGGCGT CTTCGAAGGA 
ACACGATGCT CTTCGAAAGT TCTAGGAGAT CTCCTTGATG AAAATGAAAG GCAATGTCGG 
CAAGATCCTT CTGGGTATTT ACCCGACTCT TGCAGATTCG TGCCCCATGC CAGTGGGCAA 
ACTGCCAGGG CATCGTTAAT GTTTGCTTCC AACATCCACT CGATTGACAT GTTCTGCCAC 
GACGATCCAG GAAATGTGGG GTATCATAAT TACATGGCTC CTAACCTACA GAATAAGAAG 
TGCGACTATC AAAGCGTTTG GCAGCTGATG CGGAAATCGA CTGATTTCAT GAATGGAAAC 
AGTCCTTCCT TGCCAGAGGA TACCGACACA TCACCAAACT TTATTGTCGT GCAGCCGGCC 
GGATCTCTGA GGATTGTTCT TGTTTTAGAT ACCAGTTTTT CCATGGATGG GCCCCGTTTT 
AACAAGATGA TCAGAGGGGC CAAGAACTTC ATCCAGAGTA TAGTACCTAA TAATAGTTAC 
GTGGCCATTG TCGAGTTCAA TTCCGTATCC AAAGTGAATT CTAACATGAT TGAACTCAGC 
AGTGTCATAT CAAGGAAAAA CTTGGCGTCT CTACTTCCAA CGGTTGCCGA AAACAATACG 
TGTATTGGCT GCGGCATTTT GACAGCTATC GAGGTTGCCC AATACAACGG ATTGGATTCC 
CGAGGTGTGT ATCTCATTCT TCTCAGCGAC GGACAGGAGA ATATTTATGA AGACATAACT 
CTAGTAGCTG ACACTCTGGA TGACATCGAA AGTTCAGGTG TTACTGTGCA TTCAATCGCC 
TTTTACGGAG CCGACACCCA GTTGGAAGAG CTAGCCCAGA TGACAGGCGG TAGATCGGCA 
ACGTGTGCTG ACGGAGGGAG CTCACAATGC GTCATATCCG CCTTTGTGTC AATCATAGCG 
CAAAGTCCAC AGAGTGTTGC GACTTCTGCA CCAATACAGG TTCAGAGTTC AACGATCACA 
TTAGACCCTG GCACTACCAA GACTACCGAT ATCGTCATTG ATGATTCTCT AGGACTGGAT 
ACTGTCATAA CAATTACATG GACGGCCAAC GACATCATCA CTGTCACAGT CAGAGGACCA 
GATGGTACCG TTATAGACAG TACTGATGCA AGATATGAGA CTGATATCAG CTCTAAAATT 
ATTACCGTCA CCATCATAGA TGCAGAGGCA GGTGTATGGA GCATTGAAAT CAATAACGAC 
GGCAACCCTC ACATGGAATC CGTTAGTGTG GACGTGATTT CTAAACCTCG TTCTGACGGT 
GTTTACCCTC CAGTTGTAAA TAGCTTCTTA GGGACATCGA TAGTATACTA CACCACTGCT 
CCCCTGTTAG AGGTCTATGC ATATGTCCAT ATCAACTACG TACCCGTCAT CGACGCTACA 
GTGATTGCTG AAGTAGTCAG CTCGACAACA GGGGATGTGA CAACCATCTC GCTACTTGAT 
AACGGACTAG GAGCTGATTT GATCGCAGGT GATGGTATTT ACTCGGCTTA TTTCCTTGAT 
TTCTCATCGA ATGGACGATA TGGCGTCAAG GTAGATGTAA ACGGATACAC CGCCAATTCG 
TCAGAAAGTG CGCTGCGATC TAAACGAGAT ACGGGAACTC TGACCACTCC TGTCCCGTCT 
CTACGGTTCC AGCGAACGAC TACAGCGGGC GTCTTTCAAG TAGAAAACTA CTCTCCTGAT 
CTGATTTTTG ACATCCTCGC ACCATCTAAG ATCACTGATA TCACTAGCAG AGAGAGAACA 
TACGATGAGA ATAGAAATGT GACGTTGACA TGGTCAGCTG TAGGGGATGA CTTAGACCAA 
GGAACAGCTG CCTACTACGA GCTCCGTTTC TCAGATAACT TCACCCAAAT CCGAACCAAC 
TTTACCTCCG CTGCTGCTGT TAACGATACC GATCTCATAC TTGGTAACCT CTCCCGAGTC 
GCTTCATCTG GTACTCTCGA GTCCATCACT ATTCTTCTGC CTGGTCAGGA CTCAGACGCT 
ATCTTTTCAT TCATGATAAG AGCCTGGGAT GAGGCTGGCA ACGCAGGTCC TTTATCGAAC 
ATCGTATCTG TCTCAAGACG TTCCCTACCC CCGACTACCA TGCCGACTAC CATTCTGAAC 
TCGACATCGG AACCACCATC CACACAATCA CCTGGAGTAG CACTTCCAAT ATGGGTTGTC 
ATTTCATTTT CCTGCGCCGC ATTCTTCATC GTTATCGCTG TTTCCATTAT TGTTGGTGTG 
CTTTGTAATC GTAGCGCACC TAAGAATCCA ATAGGGTCGG TTTATGAGCA CACTAATCCT 
GCTTATGAAT GTTATGAACC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC590546 ( XM_011664711.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011663013.1 Strongylocentrotus purpuratus epithelial chloride channel protein-like (LOC590546), mRNA.

Price & Availability↑

CloneID	OSd26650
Clone ID Related Accession (Same CDS sequence)	XM_011664711.1
Accession Version	XM_011664711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-23
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	epithelial chloride channel protein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011998921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 38% of CDS bases ##RefSeq-Attributes-END##

ATGCTGGGTC GAACGCCTCA GTTTGCTGCT TTTTTTCTCA GTCGCCTGAT GGGAACCAAG 
ATCTGCAAGG CCCAACAGGA TCGTAAAGTG ATTGTCCGTC AGTTTGCCAA ACTCCGTTGG 
GGCGTGTTTG ACGAGGATTA CGTGCCAGGG ACCGGTGCTG AACCTTACTA CCAATTAAAC 
TCGAACACCG TAACAGGTGG CGTCTTCGAA GGAACACGAT GCTCTTCGAA AGTTCTAGGA 
GATCTCCTTG ATGAAAATGA AAGGCAATGT CGGCAAGATC CTTCTGGGTA TTTACCCGAC 
TCTTGCAGAT TCGTGCCCCA TGCCAGTGGG CAAACTGCCA GGGCATCGTT AATGTTTGCT 
TCCAACATCC ACTCGGATAT CTTCAGGGCC GGATCTTCTA TCCTTTTCAA CGCTACCAAT 
CTGCGGGTCT ACCTTGGGAC TATCACAATC TTAGTCCCGC CCACTTGGTC CAGAAACTCT 
GAGTATCAAG TTGCCCAACG TGAAGCCTAT GAGAATGCTA ACGTTCTCGT TACTGATCAA 
CAGTCTAATC ATCGTCCGTT TGTCGATAAA CCATTCAAAT GTGGACAACA AGGAAGATTC 
ATGCATTTAT CCAAGACTTT CCTCGTCGAC CAAGCTTTAC GCGAAAATCA ATTTGGAGAT 
TCTGGTAAAG TGATTGTCCG TCAGTTTGCC AAACTCCGTT GGGGCGTGTT TGACGAGGAT 
TACGTGCCAG GGACCGATGC TGAACCTTAC TACCAATTAA ACTCGAACAC CGTAACAGGT 
GGCGTCTTCG AAGGAACACG ATGCTCTTCG AAAGTTCTAG GAGATCTCCT TGATGAAAAT 
GAAAGGCAAT GTCGGCAAGA TCCTTCTGGG TATTTACCCG ACTCTTGCAG ATTCGTGCCC 
CATGCCAGTG GGCAAACTGC CAGGGCATCG TTAATGTTTG CTTCCAACAT CCACTCGATT 
GACATGTTCT GCCACGACGA TCCAGGAAAT GTGGGGTATC ATAATTACAT GGCTCCTAAC 
CTACAGAATA AGAAGTGTGA CTATCAAAGC GTTTGGCAGC TGATGCGGAA ATCGACTGAT 
TTCATGAATG GAAACAGTCC TTCCTTGTCA GAGGATACCG ACACATCACC AAACTTTATT 
GTCGTGCAGC CGGCCGGATC TCTGAGGATT GTTCTTGTTT TAGATACCAG TTTTTCCATG 
GATGGGCCCC GTTTTAACAA GATGATCAGA GGGGCCAAGA ACTTCATCCA GAGTATAGTA 
CCTAATAATA GTTACGTGGC CATTGTCGAG TTCAATTCCG TATCCAGAGT GAATTCTCAC 
ATGATTGAAC TCAGCAGTGT CATATCAAGG AAAAACTTGG CGTCTCTACT TCCAACGGTT 
GCCGAAAACA ATACGTGTAT TGGCTGCGGC ATTTTGACAG CTATCGAGGT TGCCCAATAC 
AACGGATTGG ATTCCCGAGG TGTGTATCTC ATTCTTCTCA GCGACGGACA GGAGAATATT 
TATGAAGACA TAACTCTAGT AGCTGACACT CTGGATGACA TCGAAAGTTC AGGTGTTACT 
GTGCATTCAA TCGCCCTTTA CGGAGCCGAC ACCCAGTTGG AAGAGCTAGC CCAGATGACA 
GGCGGTAGAT CGGCAACGTG TGCTGACGGA GGGAGCTCAC AATGCGTCAT ATCCGCCTTT 
GTGTCAATCA TAGCGCAAAG TCCACAGAGT GTTGCCACTT CTGCACCAAT ACAGGTTCAG 
AGTTTAACGA TCACACTAGA CCCTGGCTTG ATCCCCACTA GTGGCACTAC CAAGACTACC 
GATATCGTTA TTGATGATTC TCTAGGACTG GACACTGTCA TGACAATTAC ATGGACGTAC 
AACGACATCA TATCCGTTAC AGTCACAGGA CCAGATGGTA CCGTTATAGA CAGTACTGAT 
GCAAGATATG ATACTGATAT GAGCTCTAAA ATTATTACCG TCACCATCAT AGATGCAGAG 
GCAGGTGTAT GGAGCATTGA AATCAATAAC GACGGCAACC CTCACATGGA ATCCGTTAGT 
GTGGACGTGA TTTCTAAACC TCGTTCTGAC GGTGTTTACC CTCCAGTTGT AAATAGCTTC 
TTAGGGACAT CGATAGTATA CTACACCACT GCTCCCCTGT TAGAGGTCTA TGCATATGTC 
CATATCAACT ACGTACCCGT CATCGACGCT ACAGTGATTG CTGAAGTAGT CAGCTCGACA 
ACAGGGGATG TGACAACCAT CTCGCTACTT GATAACGGAC TAGGAGCTGA TTTGATCGCA 
GGTGATGGTA TTTACTCGGC TTATTTCCTT GATTTCTCAT CGAATGGACG ATATGGCGTC 
AAGGTAGATG TAAACGGATA CACCGCCAAT TCGTCAGAAA GTGCGCTGCG ATCGAAACGA 
GATACGGGAA CTCTGACCAC TCCTGTCCCG TCTCTACGGT TCCAGCGAAC GACTACAGCG 
GGCGTCTTTC AAGTAGAAAA CTACTCTCCT GATCTGATTT TTGACATCCT CGCACCATCT 
AAGATCACTG ATATCACTAG CAGAGAGAGA ACATACGATG AGAATAGAAA TGTGACGTTG 
ACATGGTCAG CTGTAGGGGA TGATTTAGAC CAAGGAACAG CTGCCTACTA CGAGCTCCGT 
TTCTCAGATA ACTTCACCCA AATCCGAACC AACTTTACCT CCGCTGCTGC TGTTAACGAT 
ACCGATCTCA TACTTGGTAA ATTCTCCCGA GTCGCTTCAT CTGGTACTCT CGAGTCCATC 
ACTATTCTTC TGCCTGGTGA GGACTCAAAC GCTATCTTTT CATTCATGAT AAGAGCCTGG 
GATGAGGCTG GCAACGCAGA CAATAACCCT CTGCGATTTG TGAAGGGATT TTCACAGACT 
GCTGGTCCCC TGCACAGTCT GGTTGCCATC CAAGTTGAGA GAAGGAAAGA AAGAAAGACA 
ATACCCTTTG CATGGGAACC AGAACACCAG GCGGCATTTG AGGAGCTCAA AGAGAAACTG 
TGTCAACCGC CAGTGCTTGG CTATGCAGAC TTCACAAAAC CATTTGAGCT CGAGGTTGAT 
GCAAGTCTAC AGGGACTAGG TGCTGTTCTT TTCCAGAGAC AAGATGATGA GGGAGAAATG 
CTGGATAATG AGGAACCCAG GGAACAGTTG GAAGAATTTC AGCTTCTAGA CTGGCTGGCT 
AACCACACCA AGATTCTACA ATGGGCTTAC CAAAAAGCAT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011663013.1
CDS	1..3225
Translation	MLGRTPQFAAFFLSRLMGTKICKAQQDRKVIVRQFAKLRWGVFDEDYVPGTGAEPYYQLNSNTVTGGVFEGTRCSSKVLGDLLDENERQCRQDPSGYLPDSCRFVPHASGQTARASLMFASNIHSDIFRAGSSILFNATNLRVYLGTITILVPPTWSRNSEYQVAQREAYENANVLVTDQQSNHRPFVDKPFKCGQQGRFMHLSKTFLVDQALRENQFGDSGKVIVRQFAKLRWGVFDEDYVPGTDAEPYYQLNSNTVTGGVFEGTRCSSKVLGDLLDENERQCRQDPSGYLPDSCRFVPHASGQTARASLMFASNIHSIDMFCHDDPGNVGYHNYMAPNLQNKKCDYQSVWQLMRKSTDFMNGNSPSLSEDTDTSPNFIVVQPAGSLRIVLVLDTSFSMDGPRFNKMIRGAKNFIQSIVPNNSYVAIVEFNSVSRVNSHMIELSSVISRKNLASLLPTVAENNTCIGCGILTAIEVAQYNGLDSRGVYLILLSDGQENIYEDITLVADTLDDIESSGVTVHSIALYGADTQLEELAQMTGGRSATCADGGSSQCVISAFVSIIAQSPQSVATSAPIQVQSLTITLDPGLIPTSGTTKTTDIVIDDSLGLDTVMTITWTYNDIISVTVTGPDGTVIDSTDARYDTDMSSKIITVTIIDAEAGVWSIEINNDGNPHMESVSVDVISKPRSDGVYPPVVNSFLGTSIVYYTTAPLLEVYAYVHINYVPVIDATVIAEVVSSTTGDVTTISLLDNGLGADLIAGDGIYSAYFLDFSSNGRYGVKVDVNGYTANSSESALRSKRDTGTLTTPVPSLRFQRTTTAGVFQVENYSPDLIFDILAPSKITDITSRERTYDENRNVTLTWSAVGDDLDQGTAAYYELRFSDNFTQIRTNFTSAAAVNDTDLILGKFSRVASSGTLESITILLPGEDSNAIFSFMIRAWDEAGNADNNPLRFVKGFSQTAGPLHSLVAIQVERRKERKTIPFAWEPEHQAAFEELKEKLCQPPVLGYADFTKPFELEVDASLQGLGAVLFQRQDDEGEMLDNEEPREQLEEFQLLDWLANHTKILQWAYQKAW

Target ORF information:

RefSeq Version	XM_011664711.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus epithelial chloride channel protein-like (LOC590546), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011664711.1

ORF Insert Sequence:

ATGCTGGGTC GAACGCCTCA GTTTGCTGCT TTTTTTCTCA GTCGCCTGAT GGGAACCAAG 
ATCTGCAAGG CCCAACAGGA TCGTAAAGTG ATTGTCCGTC AGTTTGCCAA ACTCCGTTGG 
GGCGTGTTTG ACGAGGATTA CGTGCCAGGG ACCGGTGCTG AACCTTACTA CCAATTAAAC 
TCGAACACCG TAACAGGTGG CGTCTTCGAA GGAACACGAT GCTCTTCGAA AGTTCTAGGA 
GATCTCCTTG ATGAAAATGA AAGGCAATGT CGGCAAGATC CTTCTGGGTA TTTACCCGAC 
TCTTGCAGAT TCGTGCCCCA TGCCAGTGGG CAAACTGCCA GGGCATCGTT AATGTTTGCT 
TCCAACATCC ACTCGGATAT CTTCAGGGCC GGATCTTCTA TCCTTTTCAA CGCTACCAAT 
CTGCGGGTCT ACCTTGGGAC TATCACAATC TTAGTCCCGC CCACTTGGTC CAGAAACTCT 
GAGTATCAAG TTGCCCAACG TGAAGCCTAT GAGAATGCTA ACGTTCTCGT TACTGATCAA 
CAGTCTAATC ATCGTCCGTT TGTCGATAAA CCATTCAAAT GTGGACAACA AGGAAGATTC 
ATGCATTTAT CCAAGACTTT CCTCGTCGAC CAAGCTTTAC GCGAAAATCA ATTTGGAGAT 
TCTGGTAAAG TGATTGTCCG TCAGTTTGCC AAACTCCGTT GGGGCGTGTT TGACGAGGAT 
TACGTGCCAG GGACCGATGC TGAACCTTAC TACCAATTAA ACTCGAACAC CGTAACAGGT 
GGCGTCTTCG AAGGAACACG ATGCTCTTCG AAAGTTCTAG GAGATCTCCT TGATGAAAAT 
GAAAGGCAAT GTCGGCAAGA TCCTTCTGGG TATTTACCCG ACTCTTGCAG ATTCGTGCCC 
CATGCCAGTG GGCAAACTGC CAGGGCATCG TTAATGTTTG CTTCCAACAT CCACTCGATT 
GACATGTTCT GCCACGACGA TCCAGGAAAT GTGGGGTATC ATAATTACAT GGCTCCTAAC 
CTACAGAATA AGAAGTGTGA CTATCAAAGC GTTTGGCAGC TGATGCGGAA ATCGACTGAT 
TTCATGAATG GAAACAGTCC TTCCTTGTCA GAGGATACCG ACACATCACC AAACTTTATT 
GTCGTGCAGC CGGCCGGATC TCTGAGGATT GTTCTTGTTT TAGATACCAG TTTTTCCATG 
GATGGGCCCC GTTTTAACAA GATGATCAGA GGGGCCAAGA ACTTCATCCA GAGTATAGTA 
CCTAATAATA GTTACGTGGC CATTGTCGAG TTCAATTCCG TATCCAGAGT GAATTCTCAC 
ATGATTGAAC TCAGCAGTGT CATATCAAGG AAAAACTTGG CGTCTCTACT TCCAACGGTT 
GCCGAAAACA ATACGTGTAT TGGCTGCGGC ATTTTGACAG CTATCGAGGT TGCCCAATAC 
AACGGATTGG ATTCCCGAGG TGTGTATCTC ATTCTTCTCA GCGACGGACA GGAGAATATT 
TATGAAGACA TAACTCTAGT AGCTGACACT CTGGATGACA TCGAAAGTTC AGGTGTTACT 
GTGCATTCAA TCGCCCTTTA CGGAGCCGAC ACCCAGTTGG AAGAGCTAGC CCAGATGACA 
GGCGGTAGAT CGGCAACGTG TGCTGACGGA GGGAGCTCAC AATGCGTCAT ATCCGCCTTT 
GTGTCAATCA TAGCGCAAAG TCCACAGAGT GTTGCCACTT CTGCACCAAT ACAGGTTCAG 
AGTTTAACGA TCACACTAGA CCCTGGCTTG ATCCCCACTA GTGGCACTAC CAAGACTACC 
GATATCGTTA TTGATGATTC TCTAGGACTG GACACTGTCA TGACAATTAC ATGGACGTAC 
AACGACATCA TATCCGTTAC AGTCACAGGA CCAGATGGTA CCGTTATAGA CAGTACTGAT 
GCAAGATATG ATACTGATAT GAGCTCTAAA ATTATTACCG TCACCATCAT AGATGCAGAG 
GCAGGTGTAT GGAGCATTGA AATCAATAAC GACGGCAACC CTCACATGGA ATCCGTTAGT 
GTGGACGTGA TTTCTAAACC TCGTTCTGAC GGTGTTTACC CTCCAGTTGT AAATAGCTTC 
TTAGGGACAT CGATAGTATA CTACACCACT GCTCCCCTGT TAGAGGTCTA TGCATATGTC 
CATATCAACT ACGTACCCGT CATCGACGCT ACAGTGATTG CTGAAGTAGT CAGCTCGACA 
ACAGGGGATG TGACAACCAT CTCGCTACTT GATAACGGAC TAGGAGCTGA TTTGATCGCA 
GGTGATGGTA TTTACTCGGC TTATTTCCTT GATTTCTCAT CGAATGGACG ATATGGCGTC 
AAGGTAGATG TAAACGGATA CACCGCCAAT TCGTCAGAAA GTGCGCTGCG ATCGAAACGA 
GATACGGGAA CTCTGACCAC TCCTGTCCCG TCTCTACGGT TCCAGCGAAC GACTACAGCG 
GGCGTCTTTC AAGTAGAAAA CTACTCTCCT GATCTGATTT TTGACATCCT CGCACCATCT 
AAGATCACTG ATATCACTAG CAGAGAGAGA ACATACGATG AGAATAGAAA TGTGACGTTG 
ACATGGTCAG CTGTAGGGGA TGATTTAGAC CAAGGAACAG CTGCCTACTA CGAGCTCCGT 
TTCTCAGATA ACTTCACCCA AATCCGAACC AACTTTACCT CCGCTGCTGC TGTTAACGAT 
ACCGATCTCA TACTTGGTAA ATTCTCCCGA GTCGCTTCAT CTGGTACTCT CGAGTCCATC 
ACTATTCTTC TGCCTGGTGA GGACTCAAAC GCTATCTTTT CATTCATGAT AAGAGCCTGG 
GATGAGGCTG GCAACGCAGA CAATAACCCT CTGCGATTTG TGAAGGGATT TTCACAGACT 
GCTGGTCCCC TGCACAGTCT GGTTGCCATC CAAGTTGAGA GAAGGAAAGA AAGAAAGACA 
ATACCCTTTG CATGGGAACC AGAACACCAG GCGGCATTTG AGGAGCTCAA AGAGAAACTG 
TGTCAACCGC CAGTGCTTGG CTATGCAGAC TTCACAAAAC CATTTGAGCT CGAGGTTGAT 
GCAAGTCTAC AGGGACTAGG TGCTGTTCTT TTCCAGAGAC AAGATGATGA GGGAGAAATG 
CTGGATAATG AGGAACCCAG GGAACAGTTG GAAGAATTTC AGCTTCTAGA CTGGCTGGCT 
AACCACACCA AGATTCTACA ATGGGCTTAC CAAAAAGCAT GGTAG