asah2 cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asah2
Entrez Gene ID	103370157
Full Name	N-acylsphingosine amidohydrolase 2
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008299106.1	XP_008297328.1	neutral ceramidase

Pathways from BioSystems
Gene Ontology

The following asah2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asah2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt57847	XM_008299106.1 Latest version!	Stegastes partitus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (asah2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asah2 ( XM_008299106.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008297328.1 Stegastes partitus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (asah2), mRNA.

Price & Availability↑

CloneID	OSt57847
Clone ID Related Accession (Same CDS sequence)	XM_008299106.1
Accession Version	XM_008299106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	neutral ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007579112.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGGA AGTCTTCGTG TTGTGGAGCT ACAGCTCTGG AGGTGACGCT CGCCATCCTC 
TTCATCGCCA TGACGATTGT CTGTGCGACG CTCATCGCAC TGATGGTGAC CCGGACGACT 
CACACAGATC CAGAACCAAC GGCTCCTCCT CCTTCTCCTC CTCCTCAGAA CACTCCCTAC 
CTGATCGGAG TGGGCCGAGC TGACTGCACT GGACCTGCAG CCGACATCCC GCTGATGGGT 
TATGCCAACA CTCAGCAGAA GGCTGCAGGG ATTCACACTC GCCTCTACAG CAGGGCCTTC 
ATCATCGACG ACGGCACCAG AAGAGTCGTG TTTGTTACTG CAGACGTTGG GATGATCTCA 
CAGAGACTCA GGCTGGAGGT TCTGCAGGCG CTGCAGGAGA AGTACGGGGA TCAGTACCGG 
CAGGATAACG TGGTTCTGAG TGGAACTCAC ACCCACTGTG GGCCCGGAGG GTACTTCCAG 
TACACCCTGT TCATGATCAG CAGCAGCGGG TACATCAAGG CCTCGGTCCA GCCGCTGGTC 
AACGGCATCG TCAAGAGTAT AGACCGAGCC CACCAGAACA TGAGGCCCGG CCGGATCTAC 
AGGAACCGAG GAGAACTGGA CCACAGCAGC ATCAACAGAA GTCCTCACTC CTACATGAAC 
AACCCCGAGG ACGAGAGACT CAGGTATAAG GACAACACTG ATAAACAGGT GTTGGTGCTG 
AAGTTCACAG ATCTGGACGG AGACGGGATC GGTATGCTCA GCTGGTTCGC TGTCCACGCC 
GTCAGCATGA ACTACACCAA TCGCCTGGTG AGCAGCGACA ACATGGGCTA CGCCTCCTAC 
CTGCTGGAGC AGGACAAGAA CCCTGGAGAG CTGCCTGGAC AGGTGAGAGC TTTTACATGT 
ACATGTATGT CTAATACAAC ATGTGATGAA CAGACAGTAA GCAGTGCTTG TTTGTGTCAG 
ACTAAGATGT GTGCAGCGTT TGGTCCTGGA GACGACATGT TTGAGAGCAC CAGAATCATC 
GGACAGAACA TCTACATGAA GGCAAAGGAA CTCTATGCGA CTGCGGTTGA GGAGGTCACC 
GGTTCGGTCC AGTCGGCTCA TCAGTGGGTC AACATGACGG ACGTGTCTGT TCCCATCAAC 
AGCAGTCACA CGGTGAGCAC CTGTAAACCT GCTCTGGGTC ACAGCTTTGC AGCAGGAACC 
ACCGACGGAG GAGGAGACCT GAACTTCACT CAGGGTGCCG TTGAAGGCGA CCCGTTCTGG 
GACGGGATAG CCGGAGCTCT GCTGGGGCGG CCGTCCAACG AGACCAAGGA GTGTCACCGT 
CCCAAACCAA TCCTGTTCAA CACTGGAGAG ATGAACACTC CGGTGCCGTG GCATCCTCAG 
ATCGTCGATG TTCAGATCCT CACCGTCGGT TCTGTCGCCG TCGTTGCTGT TCCTGGAGAA 
ATGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008297328.1
CDS	1..1449
Translation	MGRKSSCCGATALEVTLAILFIAMTIVCATLIALMVTRTTHTDPEPTAPPPSPPPQNTPYLIGVGRADCTGPAADIPLMGYANTQQKAAGIHTRLYSRAFIIDDGTRRVVFVTADVGMISQRLRLEVLQALQEKYGDQYRQDNVVLSGTHTHCGPGGYFQYTLFMISSSGYIKASVQPLVNGIVKSIDRAHQNMRPGRIYRNRGELDHSSINRSPHSYMNNPEDERLRYKDNTDKQVLVLKFTDLDGDGIGMLSWFAVHAVSMNYTNRLVSSDNMGYASYLLEQDKNPGELPGQVRAFTCTCMSNTTCDEQTVSSACLCQTKMCAAFGPGDDMFESTRIIGQNIYMKAKELYATAVEEVTGSVQSAHQWVNMTDVSVPINSSHTVSTCKPALGHSFAAGTTDGGGDLNFTQGAVEGDPFWDGIAGALLGRPSNETKECHRPKPILFNTGEMNTPVPWHPQIVDVQILTVGSVAVVAVPGEMT

Target ORF information:

RefSeq Version	XM_008299106.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (asah2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008299106.1

ORF Insert Sequence:

ATGGGCAGGA AGTCTTCGTG TTGTGGAGCT ACAGCTCTGG AGGTGACGCT CGCCATCCTC 
TTCATCGCCA TGACGATTGT CTGTGCGACG CTCATCGCAC TGATGGTGAC CCGGACGACT 
CACACAGATC CAGAACCAAC GGCTCCTCCT CCTTCTCCTC CTCCTCAGAA CACTCCCTAC 
CTGATCGGAG TGGGCCGAGC TGACTGCACT GGACCTGCAG CCGACATCCC GCTGATGGGT 
TATGCCAACA CTCAGCAGAA GGCTGCAGGG ATTCACACTC GCCTCTACAG CAGGGCCTTC 
ATCATCGACG ACGGCACCAG AAGAGTCGTG TTTGTTACTG CAGACGTTGG GATGATCTCA 
CAGAGACTCA GGCTGGAGGT TCTGCAGGCG CTGCAGGAGA AGTACGGGGA TCAGTACCGG 
CAGGATAACG TGGTTCTGAG TGGAACTCAC ACCCACTGTG GGCCCGGAGG GTACTTCCAG 
TACACCCTGT TCATGATCAG CAGCAGCGGG TACATCAAGG CCTCGGTCCA GCCGCTGGTC 
AACGGCATCG TCAAGAGTAT AGACCGAGCC CACCAGAACA TGAGGCCCGG CCGGATCTAC 
AGGAACCGAG GAGAACTGGA CCACAGCAGC ATCAACAGAA GTCCTCACTC CTACATGAAC 
AACCCCGAGG ACGAGAGACT CAGGTATAAG GACAACACTG ATAAACAGGT GTTGGTGCTG 
AAGTTCACAG ATCTGGACGG AGACGGGATC GGTATGCTCA GCTGGTTCGC TGTCCACGCC 
GTCAGCATGA ACTACACCAA TCGCCTGGTG AGCAGCGACA ACATGGGCTA CGCCTCCTAC 
CTGCTGGAGC AGGACAAGAA CCCTGGAGAG CTGCCTGGAC AGGTGAGAGC TTTTACATGT 
ACATGTATGT CTAATACAAC ATGTGATGAA CAGACAGTAA GCAGTGCTTG TTTGTGTCAG 
ACTAAGATGT GTGCAGCGTT TGGTCCTGGA GACGACATGT TTGAGAGCAC CAGAATCATC 
GGACAGAACA TCTACATGAA GGCAAAGGAA CTCTATGCGA CTGCGGTTGA GGAGGTCACC 
GGTTCGGTCC AGTCGGCTCA TCAGTGGGTC AACATGACGG ACGTGTCTGT TCCCATCAAC 
AGCAGTCACA CGGTGAGCAC CTGTAAACCT GCTCTGGGTC ACAGCTTTGC AGCAGGAACC 
ACCGACGGAG GAGGAGACCT GAACTTCACT CAGGGTGCCG TTGAAGGCGA CCCGTTCTGG 
GACGGGATAG CCGGAGCTCT GCTGGGGCGG CCGTCCAACG AGACCAAGGA GTGTCACCGT 
CCCAAACCAA TCCTGTTCAA CACTGGAGAG ATGAACACTC CGGTGCCGTG GCATCCTCAG 
ATCGTCGATG TTCAGATCCT CACCGTCGGT TCTGTCGCCG TCGTTGCTGT TCCTGGAGAA 
ATGACGTAA