rad18 cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad18
Entrez Gene ID	103368472
Full Name	RAD18, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008296869.1	XP_008295091.1	E3 ubiquitin-protein ligase RAD18

Pathways from BioSystems
Gene Ontology

The following rad18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt56066	XM_008296869.1 Latest version!	Stegastes partitus RAD18 homolog (S. cerevisiae) (rad18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad18 ( XM_008296869.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008295091.1 Stegastes partitus RAD18 homolog (S. cerevisiae) (rad18), mRNA.

Price & Availability↑

CloneID	OSt56066
Clone ID Related Accession (Same CDS sequence)	XM_008296869.1
Accession Version	XM_008296869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	E3 ubiquitin-protein ligase RAD18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007578890.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AAATAGAAGC GGATTTACCA CCTGGCCTCG CATGTTTGAA AAATATTGAG 
GACCTGCTTC GGTGTCCCAT TTGCTTCGAC TTCCTCAACA TTTCAATGAT TACTAAATGC 
TCGCACAATT TTTGCTCCTT GTGCATCAGA AAGTTTCTTT CCTATAAGCT GCAATGTCCA 
GTTTGCAATA CACAAATGAC TGAACCAGAC CTAAGAAACA ACCGTTTATT GGATGACGTG 
GTTGTACACT TTCAAGCTGC AAGGCAGCAG TTGTCAAAAG TGAGTTTTGA GTCTCCTCCA 
ATATCACCAA AGACCCCATC TTCTGCTGTC CAATGCAAAA CTCCCAAAGA CAGGGGCCAG 
AAATGTAATA CCTCTGTCTT GAGTCATTTT TTCCAAAAGA AGTCTAAAAC CCCTCCGAGT 
AAAGAAACCC AGCGAGATGG ATCTGTCAAT CAGTTGGGGA AAATACAAAC TGCACGAACT 
CACAATGCAA AGGACGCTGA CGTACATTCA GGAAAAGCTC AACTTTCAGT GACCGTAAAG 
GAAGAACCGA TGGAGGAGGT GGACATATCT ACTAAGGGTT TGATGTCTGT CAAACAGGAG 
GATGCAACCT CGCACAGTAT TAGCACTGGA GTGGAGATGG CACATTCCTC GTCACCATCT 
CAAGACATAA AGCCCGTTAT CAAAGTGGAC TGCCCTGTGT GTTCTGTAAG TGTTTCACCA 
CAGTTTATCA ACCAGCATCT TGATGCATGT CTTACCAGAG GAGAGAAGAA GGAAAGCCTG 
AGGAGCTCCC TCGGCAGCTC GAGACGTCCA ATGGCCAAGT TGGTATACAA CCTACTTTCC 
GTGCACGAGC TGAAGCGGAG ACTGAAGGAA TGCCATCTGT CAGTGCAGGG GTCTCGAGAC 
CAGCTGATCA AAAGACACCA AGATTTTGTC CACATATACA ATGCCCAGTG TGATGCCCTA 
AACCCCAAAT CAGCTGAAGA TATTGCCAAA GAGGTGGAGC TTAATGAGAA GCGCAGAAAT 
CAACTGCATG GGAAAACCAA ACCTGTCATG GTTTTCTCAA AGAATCAGTC TGAGAAGGAG 
ATTGATGAGA TGCATTCAAA TTATAGGAAG CAGCATAGCA GTGACTTTTC CCGCCTAATT 
GCCCAGGTCC GGGGTCGCCT AGACACCAGC AGACAGACTC GTGTTAAACA GGAAGTCATT 
GTGGAGAACG AGGATGCACA AAACACACAA TCTGAAGATC AAGTTGTGGA ATCAAGTGGC 
CAAGCTGAGG AGGAATCTGC GAGAGTAATT GAGTTGTCAT CCAGCCCAGC CTATAGTGAT 
GTGTCCATTA GCAGTTCTAT TTCTGACATC TTTGGTCCAG AACCCACAGG AAACCTTGAA 
GACACGGAGA GGACCTCAGC ACAAAAGCGA GCTTCCAGGT CCGGAGGAGA TGATGCAGCT 
TCATCATCTG TTACGCGAAA GCGACGGCGT AAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008295091.1
CDS	82..1560
Translation	MAFQIEADLPPGLACLKNIEDLLRCPICFDFLNISMITKCSHNFCSLCIRKFLSYKLQCPVCNTQMTEPDLRNNRLLDDVVVHFQAARQQLSKVSFESPPISPKTPSSAVQCKTPKDRGQKCNTSVLSHFFQKKSKTPPSKETQRDGSVNQLGKIQTARTHNAKDADVHSGKAQLSVTVKEEPMEEVDISTKGLMSVKQEDATSHSISTGVEMAHSSSPSQDIKPVIKVDCPVCSVSVSPQFINQHLDACLTRGEKKESLRSSLGSSRRPMAKLVYNLLSVHELKRRLKECHLSVQGSRDQLIKRHQDFVHIYNAQCDALNPKSAEDIAKEVELNEKRRNQLHGKTKPVMVFSKNQSEKEIDEMHSNYRKQHSSDFSRLIAQVRGRLDTSRQTRVKQEVIVENEDAQNTQSEDQVVESSGQAEEESARVIELSSSPAYSDVSISSSISDIFGPEPTGNLEDTERTSAQKRASRSGGDDAASSSVTRKRRRKK

Target ORF information:

RefSeq Version	XM_008296869.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus RAD18 homolog (S. cerevisiae) (rad18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008296869.1

ORF Insert Sequence:

ATGGCATTTC AAATAGAAGC GGATTTACCA CCTGGCCTCG CATGTTTGAA AAATATTGAG 
GACCTGCTTC GGTGTCCCAT TTGCTTCGAC TTCCTCAACA TTTCAATGAT TACTAAATGC 
TCGCACAATT TTTGCTCCTT GTGCATCAGA AAGTTTCTTT CCTATAAGCT GCAATGTCCA 
GTTTGCAATA CACAAATGAC TGAACCAGAC CTAAGAAACA ACCGTTTATT GGATGACGTG 
GTTGTACACT TTCAAGCTGC AAGGCAGCAG TTGTCAAAAG TGAGTTTTGA GTCTCCTCCA 
ATATCACCAA AGACCCCATC TTCTGCTGTC CAATGCAAAA CTCCCAAAGA CAGGGGCCAG 
AAATGTAATA CCTCTGTCTT GAGTCATTTT TTCCAAAAGA AGTCTAAAAC CCCTCCGAGT 
AAAGAAACCC AGCGAGATGG ATCTGTCAAT CAGTTGGGGA AAATACAAAC TGCACGAACT 
CACAATGCAA AGGACGCTGA CGTACATTCA GGAAAAGCTC AACTTTCAGT GACCGTAAAG 
GAAGAACCGA TGGAGGAGGT GGACATATCT ACTAAGGGTT TGATGTCTGT CAAACAGGAG 
GATGCAACCT CGCACAGTAT TAGCACTGGA GTGGAGATGG CACATTCCTC GTCACCATCT 
CAAGACATAA AGCCCGTTAT CAAAGTGGAC TGCCCTGTGT GTTCTGTAAG TGTTTCACCA 
CAGTTTATCA ACCAGCATCT TGATGCATGT CTTACCAGAG GAGAGAAGAA GGAAAGCCTG 
AGGAGCTCCC TCGGCAGCTC GAGACGTCCA ATGGCCAAGT TGGTATACAA CCTACTTTCC 
GTGCACGAGC TGAAGCGGAG ACTGAAGGAA TGCCATCTGT CAGTGCAGGG GTCTCGAGAC 
CAGCTGATCA AAAGACACCA AGATTTTGTC CACATATACA ATGCCCAGTG TGATGCCCTA 
AACCCCAAAT CAGCTGAAGA TATTGCCAAA GAGGTGGAGC TTAATGAGAA GCGCAGAAAT 
CAACTGCATG GGAAAACCAA ACCTGTCATG GTTTTCTCAA AGAATCAGTC TGAGAAGGAG 
ATTGATGAGA TGCATTCAAA TTATAGGAAG CAGCATAGCA GTGACTTTTC CCGCCTAATT 
GCCCAGGTCC GGGGTCGCCT AGACACCAGC AGACAGACTC GTGTTAAACA GGAAGTCATT 
GTGGAGAACG AGGATGCACA AAACACACAA TCTGAAGATC AAGTTGTGGA ATCAAGTGGC 
CAAGCTGAGG AGGAATCTGC GAGAGTAATT GAGTTGTCAT CCAGCCCAGC CTATAGTGAT 
GTGTCCATTA GCAGTTCTAT TTCTGACATC TTTGGTCCAG AACCCACAGG AAACCTTGAA 
GACACGGAGA GGACCTCAGC ACAAAAGCGA GCTTCCAGGT CCGGAGGAGA TGATGCAGCT 
TCATCATCTG TTACGCGAAA GCGACGGCGT AAAAAGTAA