usp25 cDNA ORF clone, Stegastes partitus(bicolor damselfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp25
Entrez Gene ID	103363750
Full Name	ubiquitin specific peptidase 25
Gene Type	protein-coding
Organism	Stegastes partitus(bicolor damselfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008290633.1	XP_008288855.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_008290634.1	XP_008288856.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2

Pathways from BioSystems
Gene Ontology

The following usp25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt50989	XM_008290633.1 Latest version!	Stegastes partitus ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OSt50990	XM_008290634.1 Latest version!	Stegastes partitus ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp25 ( XM_008290633.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008288855.1 Stegastes partitus ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSt50989
Clone ID Related Accession (Same CDS sequence)	XM_008290633.1
Accession Version	XM_008290633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007578418.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTCCAGCAG CATTCTCAGA AGCACCAGCA GACGCTACTA 
AACCAGTTGA AGGAGGTCAC AGGCACAACA GACGTTCAGC TGCTTCAGCA GGCCCTGCAG 
GTGAGCAATG GAGACCTGGC TGAGGCTGTG GCCTTTCTGA CAGAGAAGAA CGCTAAGGTT 
CCTCAGCAAG ATGAAACTAC CTACTACCAG ACGTCCCAGG TGGCCAGCGA CAGATACATC 
AGCGTGGGCA GCCAGGCAGA CACAAACGTG ATCGATCTGA CTGGGGACGA TAAAGATGAC 
CTGCAGAGGG CTATCGCCCT CAGCCTGGAG GAGTCCAACC GGGCGTTCAG GGAGACGGGC 
ATCACTGATG AAGAGCAGGC CATCAGCAGA GTTTTGGAGG CCAGCATAGC AGAGAACAAG 
GCAAGTCTAA AGCGTACCCA CACAGAAGTA TGGAGTGATT CACCCAACCC ACATGACAGG 
AAGAGGATAG ACAACTGCCC TGTGGGTCTG AAAAATGTTG GCAACACCTG CTGGTTCAGT 
GCTGTCATAC AGTCTCTGTT CAACCTGCTG GAGTTCCAGA GGCTGGTGCT CAACTACTCA 
CCGCCAGCCA GAGTCCATGA CCTGCCTCGC AACCAGAAGG AGCACAGAAA CCTGCCCTTC 
ATGCAGGAGC TTAGGAACCT CTTCTCACTC ATGGTGGGTT CTAAGAGGAA ATATGTGGAT 
CCATCGCGAG CCGTGGAAAT CCTCAAAGAC GCCTTCAAGT CCAGTGAGTC ACAGCAGCAG 
GATGTGAGCG AGTTCACCCA CAAGCTGCTG GACTGGCTGG AGGACGCCTT CCAGATGAAG 
GCTGAAGAGG ACAGGGAGGG CGAGAAGCCA AAGAACCCCA TGGTGGAGCT TTTCTACGGG 
CGCTTCCTTG CTGTGGGCGT CCTGGAAGGT AAAAAATTTG AGAACACCGA GATGTTTGGA 
CAGTACCCCC TGCAGGTGAA CGGCTTCAAG GACCTCCACG AGTGTCTCGA GGCAGCGATG 
ATCGAGGGCG AGATCGAGTC CCTGCACTCG GCAGAGAACT CGGCCAGGTC TGGACAGGAG 
CACTGGTTCA CCGAACTCCC TCCCGTGTTG ACCTTCGAGC TGTCGAGGTT TGAGTTCAAC 
CAGGCGCTAG GACGACCCGA GAAGATCCAC AACAAGCTGG AGTTCCCCTC GATGCTCTAC 
ATGGACAGGT ACATGGACAG GAACAGAGAA ATAACCCGGA TCAAGAGAGA GGAGATCAGG 
AGGCTGAAGG AGCATCTGAC GCTGCTCCAA CAGCGACTGG AGAGGTATCT GAGTTATGGC 
TCAGGCCCCA AGAGGTTTCC TCTGGCAGAT GTCCTCCAGT ACGCCATGGA GTTTGCCTCC 
AGTAAGCCTG TGTGTACCTC CCCAGTGGAA GACATTGACA CATCGGCGCC CCCCGGTGGC 
ACAACGGGGC AACAGCTACC CCCACCAAGC ACAGCTGAGC AGGACTCCTT ACCGCCCTTA 
GAGAGCTCAG GCCCTGCTTC CGGTCCCACC ACAGCTCCCA CTGCACCCCA GCAGCAGAGA 
GTACCCATCC ACAAGCCCTT CACCCAGTCC CGACTACCTC CGGACCTCCC CATGCACCCC 
GCCCCCCGCC ACATCACGGA GGAAGAGCTG AGGGTGCTGG AGGGCTGCTT GCATCGCTGG 
AGGAGCGAAG TGGAAAATGA CACGCGTGAC CTGCAGGGCA GCATAACCAG AATCCAAAGA 
ACCATCGAAC TCATGTACTC TGACAAATCT ATGATGCAGG TTCCATACCG GCTCCACGCG 
GTGCTGGTCC ACGAAGGCCA GGCCAACGCG GGTCACTACT GGGCTTACAT CTACGACCCA 
CATCAGCGTT GCTGGATGAA GTACAACGAC ATTTCGGTCA CCAAGTCGTC CTGGGAGGAG 
CTGGTCAGGG ATTCTTTTGG AGGCTACCGC AACGCCAGTG CCTACTGTCT CATGTACATC 
AACGACAAGA AGCCATTTCT CATAGAAGAG GAGTTTGATA AAGAAACCGG GCAGATACTC 
AGCGGGATGG ACAAGCTGCC CCCGGACCTG AAGCTGTACG TGAAGGAGGA CAACGAGCAG 
TTTGACAAGG AGATTGAAGA GTGGGACGCC CTGCAGGCTC GCAAGGCCCA GCAGGAGAAG 
CTGGCCTTGG CCACAGCGGC GGCGGCGGCC GCTGCCTCGG CAGCATCCAG CACCTCGACT 
TCTTCTTCTT CCCCTCAGCC TATGAGCATC GAGTCCAGCC CTCCAGACAA CGCAGTCCCC 
CAGCAGGACC CGGAGTACAT GGAGCAGCCG TCGCCCACAA ATGACTCCAA GCACCTGCAG 
GAGGACACTG AGAGGGCCAT CTCCAGGGCT GCAGCCGAAC AGGAGGAGAG GAGCCCCGAA 
GCGTTGCTAA ATGCTTCTCC TCCCCCTCCC ACCTCCCTTC AGCCTGAGGT CCAGATGGGC 
CCTGCCCCTA GCCCTCCTCC TGACCTGGTC ACAGAGGATG AGCCCCCTGG CCAGCGCACC 
GTAGAGGTGG CCATTCCTAA CGTGGGGACC TTTGTTATTG AGTCAGAGGA GGGGGGTTAT 
GATGACGAGA CAATGATGAC CCCCAACATG CAGGGTATAA TTATGGCCAT TGGCAAATCC 
GGCAGCGTGT ATGAAAAGAA TGGGCCTGAG GCAGCCTTTT TTAAGGCCAT CAAGCTGGAG 
TACGGTCGTC TTCTGAGATT CGCCCAAGAA GACACCCCGC CTGAGAACGA CTACCGTCTG 
CAGCACATCA TCGTCTACTT CATCCAAAAC CAGGCGCCCA AGAAGATCCT GGAGAGGACT 
CTGCTGACAC AGTTTGCAGA CAGGAACCTG GCTTTTGATG AGAGATGTAA GAGCATTATG 
AATGTGGCAC GTGCCAAACT GGACCTAATT AAGCCGGAGG AGGTCAACAT GGACGAATAT 
GAGATGTGGC ATCAGGACTA CAGGAATTTC CGTGAGACAA CCGTCTTCAT GATGACCGGC 
TTGGAGCTCT TCCAGAAAAC AAATTACGTG GAGGCTCTGA TGTATCTGAT CTACGCATAC 
CAGTACAACA AGGAGCTGCT GTTGAAGGGG CTGTACAGGG GGCACGACGA GGAGCTGCTG 
GGGCATTACC GCCGAGAGTG TCTGCTGAAA CTAAACGAGC AAGCGGCCGC CATGTTCGAG 
TCGGGAGAAG AACCTGAGGT GACCACGGGC CTGGGCATCA TGAACGAGCT GGTGGTGCCC 
TGCATCCCCC TGCTGCTGGT CCACGACACG GAGAGGGACC TGCTGGCGGT GGAGGACATG 
AGGAACCGCT GGTGCTCCTA CCTAGGTCAA GAGATGGAAT CCAACCTCCA GGAAAAGCTG 
ACGGACTTCC TCCCCAAGTT ACTGGACTGC TCTACGGAGA TCAAAAGCTT CCACGACCCT 
CCCAAGCTGC CCTCCTACTC CACCCTGGAG CTGTGCGAAC GATTCAGCCG CGTCATGGCC 
GCCCTCGGCA GGGTGCCCTC CGAGGGCAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008288855.1
CDS	199..3711
Translation	MTVEQNVLQQHSQKHQQTLLNQLKEVTGTTDVQLLQQALQVSNGDLAEAVAFLTEKNAKVPQQDETTYYQTSQVASDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHDRKRIDNCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLNYSPPARVHDLPRNQKEHRNLPFMQELRNLFSLMVGSKRKYVDPSRAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQMKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSARSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTLLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTSAPPGGTTGQQLPPPSTAEQDSLPPLESSGPASGPTTAPTAPQQQRVPIHKPFTQSRLPPDLPMHPAPRHITEEELRVLEGCLHRWRSEVENDTRDLQGSITRIQRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDPHQRCWMKYNDISVTKSSWEELVRDSFGGYRNASAYCLMYINDKKPFLIEEEFDKETGQILSGMDKLPPDLKLYVKEDNEQFDKEIEEWDALQARKAQQEKLALATAAAAAAASAASSTSTSSSSPQPMSIESSPPDNAVPQQDPEYMEQPSPTNDSKHLQEDTERAISRAAAEQEERSPEALLNASPPPPTSLQPEVQMGPAPSPPPDLVTEDEPPGQRTVEVAIPNVGTFVIESEEGGYDDETMMTPNMQGIIMAIGKSGSVYEKNGPEAAFFKAIKLEYGRLLRFAQEDTPPENDYRLQHIIVYFIQNQAPKKILERTLLTQFADRNLAFDERCKSIMNVARAKLDLIKPEEVNMDEYEMWHQDYRNFRETTVFMMTGLELFQKTNYVEALMYLIYAYQYNKELLLKGLYRGHDEELLGHYRRECLLKLNEQAAAMFESGEEPEVTTGLGIMNELVVPCIPLLLVHDTERDLLAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKSFHDPPKLPSYSTLELCERFSRVMAALGRVPSEGR

Target ORF information:

RefSeq Version	XM_008290633.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008290633.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTCCAGCAG CATTCTCAGA AGCACCAGCA GACGCTACTA 
AACCAGTTGA AGGAGGTCAC AGGCACAACA GACGTTCAGC TGCTTCAGCA GGCCCTGCAG 
GTGAGCAATG GAGACCTGGC TGAGGCTGTG GCCTTTCTGA CAGAGAAGAA CGCTAAGGTT 
CCTCAGCAAG ATGAAACTAC CTACTACCAG ACGTCCCAGG TGGCCAGCGA CAGATACATC 
AGCGTGGGCA GCCAGGCAGA CACAAACGTG ATCGATCTGA CTGGGGACGA TAAAGATGAC 
CTGCAGAGGG CTATCGCCCT CAGCCTGGAG GAGTCCAACC GGGCGTTCAG GGAGACGGGC 
ATCACTGATG AAGAGCAGGC CATCAGCAGA GTTTTGGAGG CCAGCATAGC AGAGAACAAG 
GCAAGTCTAA AGCGTACCCA CACAGAAGTA TGGAGTGATT CACCCAACCC ACATGACAGG 
AAGAGGATAG ACAACTGCCC TGTGGGTCTG AAAAATGTTG GCAACACCTG CTGGTTCAGT 
GCTGTCATAC AGTCTCTGTT CAACCTGCTG GAGTTCCAGA GGCTGGTGCT CAACTACTCA 
CCGCCAGCCA GAGTCCATGA CCTGCCTCGC AACCAGAAGG AGCACAGAAA CCTGCCCTTC 
ATGCAGGAGC TTAGGAACCT CTTCTCACTC ATGGTGGGTT CTAAGAGGAA ATATGTGGAT 
CCATCGCGAG CCGTGGAAAT CCTCAAAGAC GCCTTCAAGT CCAGTGAGTC ACAGCAGCAG 
GATGTGAGCG AGTTCACCCA CAAGCTGCTG GACTGGCTGG AGGACGCCTT CCAGATGAAG 
GCTGAAGAGG ACAGGGAGGG CGAGAAGCCA AAGAACCCCA TGGTGGAGCT TTTCTACGGG 
CGCTTCCTTG CTGTGGGCGT CCTGGAAGGT AAAAAATTTG AGAACACCGA GATGTTTGGA 
CAGTACCCCC TGCAGGTGAA CGGCTTCAAG GACCTCCACG AGTGTCTCGA GGCAGCGATG 
ATCGAGGGCG AGATCGAGTC CCTGCACTCG GCAGAGAACT CGGCCAGGTC TGGACAGGAG 
CACTGGTTCA CCGAACTCCC TCCCGTGTTG ACCTTCGAGC TGTCGAGGTT TGAGTTCAAC 
CAGGCGCTAG GACGACCCGA GAAGATCCAC AACAAGCTGG AGTTCCCCTC GATGCTCTAC 
ATGGACAGGT ACATGGACAG GAACAGAGAA ATAACCCGGA TCAAGAGAGA GGAGATCAGG 
AGGCTGAAGG AGCATCTGAC GCTGCTCCAA CAGCGACTGG AGAGGTATCT GAGTTATGGC 
TCAGGCCCCA AGAGGTTTCC TCTGGCAGAT GTCCTCCAGT ACGCCATGGA GTTTGCCTCC 
AGTAAGCCTG TGTGTACCTC CCCAGTGGAA GACATTGACA CATCGGCGCC CCCCGGTGGC 
ACAACGGGGC AACAGCTACC CCCACCAAGC ACAGCTGAGC AGGACTCCTT ACCGCCCTTA 
GAGAGCTCAG GCCCTGCTTC CGGTCCCACC ACAGCTCCCA CTGCACCCCA GCAGCAGAGA 
GTACCCATCC ACAAGCCCTT CACCCAGTCC CGACTACCTC CGGACCTCCC CATGCACCCC 
GCCCCCCGCC ACATCACGGA GGAAGAGCTG AGGGTGCTGG AGGGCTGCTT GCATCGCTGG 
AGGAGCGAAG TGGAAAATGA CACGCGTGAC CTGCAGGGCA GCATAACCAG AATCCAAAGA 
ACCATCGAAC TCATGTACTC TGACAAATCT ATGATGCAGG TTCCATACCG GCTCCACGCG 
GTGCTGGTCC ACGAAGGCCA GGCCAACGCG GGTCACTACT GGGCTTACAT CTACGACCCA 
CATCAGCGTT GCTGGATGAA GTACAACGAC ATTTCGGTCA CCAAGTCGTC CTGGGAGGAG 
CTGGTCAGGG ATTCTTTTGG AGGCTACCGC AACGCCAGTG CCTACTGTCT CATGTACATC 
AACGACAAGA AGCCATTTCT CATAGAAGAG GAGTTTGATA AAGAAACCGG GCAGATACTC 
AGCGGGATGG ACAAGCTGCC CCCGGACCTG AAGCTGTACG TGAAGGAGGA CAACGAGCAG 
TTTGACAAGG AGATTGAAGA GTGGGACGCC CTGCAGGCTC GCAAGGCCCA GCAGGAGAAG 
CTGGCCTTGG CCACAGCGGC GGCGGCGGCC GCTGCCTCGG CAGCATCCAG CACCTCGACT 
TCTTCTTCTT CCCCTCAGCC TATGAGCATC GAGTCCAGCC CTCCAGACAA CGCAGTCCCC 
CAGCAGGACC CGGAGTACAT GGAGCAGCCG TCGCCCACAA ATGACTCCAA GCACCTGCAG 
GAGGACACTG AGAGGGCCAT CTCCAGGGCT GCAGCCGAAC AGGAGGAGAG GAGCCCCGAA 
GCGTTGCTAA ATGCTTCTCC TCCCCCTCCC ACCTCCCTTC AGCCTGAGGT CCAGATGGGC 
CCTGCCCCTA GCCCTCCTCC TGACCTGGTC ACAGAGGATG AGCCCCCTGG CCAGCGCACC 
GTAGAGGTGG CCATTCCTAA CGTGGGGACC TTTGTTATTG AGTCAGAGGA GGGGGGTTAT 
GATGACGAGA CAATGATGAC CCCCAACATG CAGGGTATAA TTATGGCCAT TGGCAAATCC 
GGCAGCGTGT ATGAAAAGAA TGGGCCTGAG GCAGCCTTTT TTAAGGCCAT CAAGCTGGAG 
TACGGTCGTC TTCTGAGATT CGCCCAAGAA GACACCCCGC CTGAGAACGA CTACCGTCTG 
CAGCACATCA TCGTCTACTT CATCCAAAAC CAGGCGCCCA AGAAGATCCT GGAGAGGACT 
CTGCTGACAC AGTTTGCAGA CAGGAACCTG GCTTTTGATG AGAGATGTAA GAGCATTATG 
AATGTGGCAC GTGCCAAACT GGACCTAATT AAGCCGGAGG AGGTCAACAT GGACGAATAT 
GAGATGTGGC ATCAGGACTA CAGGAATTTC CGTGAGACAA CCGTCTTCAT GATGACCGGC 
TTGGAGCTCT TCCAGAAAAC AAATTACGTG GAGGCTCTGA TGTATCTGAT CTACGCATAC 
CAGTACAACA AGGAGCTGCT GTTGAAGGGG CTGTACAGGG GGCACGACGA GGAGCTGCTG 
GGGCATTACC GCCGAGAGTG TCTGCTGAAA CTAAACGAGC AAGCGGCCGC CATGTTCGAG 
TCGGGAGAAG AACCTGAGGT GACCACGGGC CTGGGCATCA TGAACGAGCT GGTGGTGCCC 
TGCATCCCCC TGCTGCTGGT CCACGACACG GAGAGGGACC TGCTGGCGGT GGAGGACATG 
AGGAACCGCT GGTGCTCCTA CCTAGGTCAA GAGATGGAAT CCAACCTCCA GGAAAAGCTG 
ACGGACTTCC TCCCCAAGTT ACTGGACTGC TCTACGGAGA TCAAAAGCTT CCACGACCCT 
CCCAAGCTGC CCTCCTACTC CACCCTGGAG CTGTGCGAAC GATTCAGCCG CGTCATGGCC 
GCCCTCGGCA GGGTGCCCTC CGAGGGCAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp25 ( XM_008290634.1 ) cDNA ORF clone, Stegastes partitus(bicolor damselfish) -> XP_008288856.1 Stegastes partitus ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSt50990
Clone ID Related Accession (Same CDS sequence)	XM_008290634.1
Accession Version	XM_008290634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-06-18
Organism	Stegastes partitus(bicolor damselfish)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007578418.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Stegastes partitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT CCTCCAGCAG CATTCTCAGA AGCACCAGCA GACGCTACTA 
AACCAGTTGA AGGAGGTCAC AGGCACAACA GACGTTCAGC TGCTTCAGCA GGCCCTGCAG 
GTGAGCAATG GAGACCTGGC TGAGGCTGTG GCCTTTCTGA CAGAGAAGAA CGCTAAGGTT 
CCTCAGCAAG ATGAAACTAC CTACTACCAG ACGTCCCAGG TGGCCAGCGA CAGATACATC 
AGCGTGGGCA GCCAGGCAGA CACAAACGTG ATCGATCTGA CTGGGGACGA TAAAGATGAC 
CTGCAGAGGG CTATCGCCCT CAGCCTGGAG GAGTCCAACC GGGCGTTCAG GGAGACGGGC 
ATCACTGATG AAGAGCAGGC CATCAGCAGA GTTTTGGAGG CCAGCATAGC AGAGAACAAG 
GCAAGTCTAA AGCGTACCCA CACAGAAGTA TGGAGTGATT CACCCAACCC ACATGACAGG 
AAGAGGATAG ACAACTGCCC TGTGGGTCTG AAAAATGTTG GCAACACCTG CTGGTTCAGT 
GCTGTCATAC AGTCTCTGTT CAACCTGCTG GAGTTCCAGA GGCTGGTGCT CAACTACTCA 
CCGCCAGCCA GAGTCCATGA CCTGCCTCGC AACCAGAAGG AGCACAGAAA CCTGCCCTTC 
ATGCAGGAGC TTAGGAACCT CTTCTCACTC ATGGTGGGTT CTAAGAGGAA ATATGTGGAT 
CCATCGCGAG CCGTGGAAAT CCTCAAAGAC GCCTTCAAGT CCAGTGAGTC ACAGCAGCAG 
GATGTGAGCG AGTTCACCCA CAAGCTGCTG GACTGGCTGG AGGACGCCTT CCAGATGAAG 
GCTGAAGAGG ACAGGGAGGG CGAGAAGCCA AAGAACCCCA TGGTGGAGCT TTTCTACGGG 
CGCTTCCTTG CTGTGGGCGT CCTGGAAGGT AAAAAATTTG AGAACACCGA GATGTTTGGA 
CAGTACCCCC TGCAGGTGAA CGGCTTCAAG GACCTCCACG AGTGTCTCGA GGCAGCGATG 
ATCGAGGGCG AGATCGAGTC CCTGCACTCG GCAGAGAACT CGGCCAGGTC TGGACAGGAG 
CACTGGTTCA CCGAACTCCC TCCCGTGTTG ACCTTCGAGC TGTCGAGGTT TGAGTTCAAC 
CAGGCGCTAG GACGACCCGA GAAGATCCAC AACAAGCTGG AGTTCCCCTC GATGCTCTAC 
ATGGACAGGT ACATGGACAG GAACAGAGAA ATAACCCGGA TCAAGAGAGA GGAGATCAGG 
AGGCTGAAGG AGCATCTGAC GCTGCTCCAA CAGCGACTGG AGAGGTATCT GAGTTATGGC 
TCAGGCCCCA AGAGGTTTCC TCTGGCAGAT GTCCTCCAGT ACGCCATGGA GTTTGCCTCC 
AGTAAGCCTG TGTGTACCTC CCCAGTGGAA GACATTGACA CATCGGCGCC CCCCGGTGGC 
ACAACGGGGC AACAGCTACC CCCACCAAGC ACAGCTGAGC AGGACTCCTT ACCGCCCTTA 
GAGAGCTCAG GCCCTGCTTC CGGTCCCACC ACAGCTCCCA CTGCACCCCA GCAGCAGAGA 
GTACCCATCC ACAAGCCCTT CACCCAGTCC CGACTACCTC CGGACCTCCC CATGCACCCC 
GCCCCCCGCC ACATCACGGA GGAAGAGCTG AGGGTGCTGG AGGGCTGCTT GCATCGCTGG 
AGGAGCGAAG TGGAAAATGA CACGCGTGAC CTGCAGGGCA GCATAACCAG AATCCAAAGA 
ACCATCGAAC TCATGTACTC TGACAAATCT ATGATGCAGG TTCCATACCG GCTCCACGCG 
GTGCTGGTCC ACGAAGGCCA GGCCAACGCG GGTCACTACT GGGCTTACAT CTACGACCCA 
CATCAGCGTT GCTGGATGAA GTACAACGAC ATTTCGGTCA CCAAGTCGTC CTGGGAGGAG 
CTGGTCAGGG ATTCTTTTGG AGGCTACCGC AACGCCAGTG CCTACTGTCT CATGTACATC 
AACGACAAGA AGCCATTTCT CATAGAAGAG GAGTTTGATA AAGAAACCGG GCAGATACTC 
AGCGGGATGG ACAAGCTGCC CCCGGACCTG AAGCTGTACG TGAAGGAGGA CAACGAGCAG 
TTTGACAAGG AGATTGAAGA GTGGGACGCC CTGCAGGCTC GCAAGGCCCA GCAGGAGAAG 
CTGGCCTTGG CCACAGCGGC GGCGGCGGCC GCTGCCTCGG CAGCATCCAG CACCTCGACT 
TCTTCTTCTT CCCCTCAGCC TATGAGCATC GAGTCCAGCC CTCCAGACAA CGCAGTCCCC 
CAGCAGGACC CGGAGTACAT GGAGCAGCCG TCGCCCACAA ATGACTCCAA GCACCTGCAG 
GAGGACACTG AGAGGGCCAT CTCCAGGGCT GCAGCCGAAC AGGAGGAGAG GAGCCCCGAA 
GCGTTGCTAA ATGCTGCCAT CAAGCTGGAG TACGGTCGTC TTCTGAGATT CGCCCAAGAA 
GACACCCCGC CTGAGAACGA CTACCGTCTG CAGCACATCA TCGTCTACTT CATCCAAAAC 
CAGGCGCCCA AGAAGATCCT GGAGAGGACT CTGCTGACAC AGTTTGCAGA CAGGAACCTG 
GCTTTTGATG AGAGATGTAA GAGCATTATG AATGTGGCAC GTGCCAAACT GGACCTAATT 
AAGCCGGAGG AGGTCAACAT GGACGAATAT GAGATGTGGC ATCAGGACTA CAGGAATTTC 
CGTGAGACAA CCGTCTTCAT GATGACCGGC TTGGAGCTCT TCCAGAAAAC AAATTACGTG 
GAGGCTCTGA TGTATCTGAT CTACGCATAC CAGTACAACA AGGAGCTGCT GTTGAAGGGG 
CTGTACAGGG GGCACGACGA GGAGCTGCTG GGGCATTACC GCCGAGAGTG TCTGCTGAAA 
CTAAACGAGC AAGCGGCCGC CATGTTCGAG TCGGGAGAAG AACCTGAGGT GACCACGGGC 
CTGGGCATCA TGAACGAGCT GGTGGTGCCC TGCATCCCCC TGCTGCTGGT CCACGACACG 
GAGAGGGACC TGCTGGCGGT GGAGGACATG AGGAACCGCT GGTGCTCCTA CCTAGGTCAA 
GAGATGGAAT CCAACCTCCA GGAAAAGCTG ACGGACTTCC TCCCCAAGTT ACTGGACTGC 
TCTACGGAGA TCAAAAGCTT CCACGACCCT CCCAAGCTGC CCTCCTACTC CACCCTGGAG 
CTGTGCGAAC GATTCAGCCG CGTCATGGCC GCCCTCGGCA GGGTGCCCTC CGAGGGCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008288856.1
CDS	199..3441
Translation	MTVEQNVLQQHSQKHQQTLLNQLKEVTGTTDVQLLQQALQVSNGDLAEAVAFLTEKNAKVPQQDETTYYQTSQVASDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLEESNRAFRETGITDEEQAISRVLEASIAENKASLKRTHTEVWSDSPNPHDRKRIDNCPVGLKNVGNTCWFSAVIQSLFNLLEFQRLVLNYSPPARVHDLPRNQKEHRNLPFMQELRNLFSLMVGSKRKYVDPSRAVEILKDAFKSSESQQQDVSEFTHKLLDWLEDAFQMKAEEDREGEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSAENSARSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPSMLYMDRYMDRNREITRIKREEIRRLKEHLTLLQQRLERYLSYGSGPKRFPLADVLQYAMEFASSKPVCTSPVEDIDTSAPPGGTTGQQLPPPSTAEQDSLPPLESSGPASGPTTAPTAPQQQRVPIHKPFTQSRLPPDLPMHPAPRHITEEELRVLEGCLHRWRSEVENDTRDLQGSITRIQRTIELMYSDKSMMQVPYRLHAVLVHEGQANAGHYWAYIYDPHQRCWMKYNDISVTKSSWEELVRDSFGGYRNASAYCLMYINDKKPFLIEEEFDKETGQILSGMDKLPPDLKLYVKEDNEQFDKEIEEWDALQARKAQQEKLALATAAAAAAASAASSTSTSSSSPQPMSIESSPPDNAVPQQDPEYMEQPSPTNDSKHLQEDTERAISRAAAEQEERSPEALLNAAIKLEYGRLLRFAQEDTPPENDYRLQHIIVYFIQNQAPKKILERTLLTQFADRNLAFDERCKSIMNVARAKLDLIKPEEVNMDEYEMWHQDYRNFRETTVFMMTGLELFQKTNYVEALMYLIYAYQYNKELLLKGLYRGHDEELLGHYRRECLLKLNEQAAAMFESGEEPEVTTGLGIMNELVVPCIPLLLVHDTERDLLAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKSFHDPPKLPSYSTLELCERFSRVMAALGRVPSEGR

Target ORF information:

RefSeq Version	XM_008290634.1
Organism	Stegastes partitus(bicolor damselfish)
Definition	Stegastes partitus ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008290634.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT CCTCCAGCAG CATTCTCAGA AGCACCAGCA GACGCTACTA 
AACCAGTTGA AGGAGGTCAC AGGCACAACA GACGTTCAGC TGCTTCAGCA GGCCCTGCAG 
GTGAGCAATG GAGACCTGGC TGAGGCTGTG GCCTTTCTGA CAGAGAAGAA CGCTAAGGTT 
CCTCAGCAAG ATGAAACTAC CTACTACCAG ACGTCCCAGG TGGCCAGCGA CAGATACATC 
AGCGTGGGCA GCCAGGCAGA CACAAACGTG ATCGATCTGA CTGGGGACGA TAAAGATGAC 
CTGCAGAGGG CTATCGCCCT CAGCCTGGAG GAGTCCAACC GGGCGTTCAG GGAGACGGGC 
ATCACTGATG AAGAGCAGGC CATCAGCAGA GTTTTGGAGG CCAGCATAGC AGAGAACAAG 
GCAAGTCTAA AGCGTACCCA CACAGAAGTA TGGAGTGATT CACCCAACCC ACATGACAGG 
AAGAGGATAG ACAACTGCCC TGTGGGTCTG AAAAATGTTG GCAACACCTG CTGGTTCAGT 
GCTGTCATAC AGTCTCTGTT CAACCTGCTG GAGTTCCAGA GGCTGGTGCT CAACTACTCA 
CCGCCAGCCA GAGTCCATGA CCTGCCTCGC AACCAGAAGG AGCACAGAAA CCTGCCCTTC 
ATGCAGGAGC TTAGGAACCT CTTCTCACTC ATGGTGGGTT CTAAGAGGAA ATATGTGGAT 
CCATCGCGAG CCGTGGAAAT CCTCAAAGAC GCCTTCAAGT CCAGTGAGTC ACAGCAGCAG 
GATGTGAGCG AGTTCACCCA CAAGCTGCTG GACTGGCTGG AGGACGCCTT CCAGATGAAG 
GCTGAAGAGG ACAGGGAGGG CGAGAAGCCA AAGAACCCCA TGGTGGAGCT TTTCTACGGG 
CGCTTCCTTG CTGTGGGCGT CCTGGAAGGT AAAAAATTTG AGAACACCGA GATGTTTGGA 
CAGTACCCCC TGCAGGTGAA CGGCTTCAAG GACCTCCACG AGTGTCTCGA GGCAGCGATG 
ATCGAGGGCG AGATCGAGTC CCTGCACTCG GCAGAGAACT CGGCCAGGTC TGGACAGGAG 
CACTGGTTCA CCGAACTCCC TCCCGTGTTG ACCTTCGAGC TGTCGAGGTT TGAGTTCAAC 
CAGGCGCTAG GACGACCCGA GAAGATCCAC AACAAGCTGG AGTTCCCCTC GATGCTCTAC 
ATGGACAGGT ACATGGACAG GAACAGAGAA ATAACCCGGA TCAAGAGAGA GGAGATCAGG 
AGGCTGAAGG AGCATCTGAC GCTGCTCCAA CAGCGACTGG AGAGGTATCT GAGTTATGGC 
TCAGGCCCCA AGAGGTTTCC TCTGGCAGAT GTCCTCCAGT ACGCCATGGA GTTTGCCTCC 
AGTAAGCCTG TGTGTACCTC CCCAGTGGAA GACATTGACA CATCGGCGCC CCCCGGTGGC 
ACAACGGGGC AACAGCTACC CCCACCAAGC ACAGCTGAGC AGGACTCCTT ACCGCCCTTA 
GAGAGCTCAG GCCCTGCTTC CGGTCCCACC ACAGCTCCCA CTGCACCCCA GCAGCAGAGA 
GTACCCATCC ACAAGCCCTT CACCCAGTCC CGACTACCTC CGGACCTCCC CATGCACCCC 
GCCCCCCGCC ACATCACGGA GGAAGAGCTG AGGGTGCTGG AGGGCTGCTT GCATCGCTGG 
AGGAGCGAAG TGGAAAATGA CACGCGTGAC CTGCAGGGCA GCATAACCAG AATCCAAAGA 
ACCATCGAAC TCATGTACTC TGACAAATCT ATGATGCAGG TTCCATACCG GCTCCACGCG 
GTGCTGGTCC ACGAAGGCCA GGCCAACGCG GGTCACTACT GGGCTTACAT CTACGACCCA 
CATCAGCGTT GCTGGATGAA GTACAACGAC ATTTCGGTCA CCAAGTCGTC CTGGGAGGAG 
CTGGTCAGGG ATTCTTTTGG AGGCTACCGC AACGCCAGTG CCTACTGTCT CATGTACATC 
AACGACAAGA AGCCATTTCT CATAGAAGAG GAGTTTGATA AAGAAACCGG GCAGATACTC 
AGCGGGATGG ACAAGCTGCC CCCGGACCTG AAGCTGTACG TGAAGGAGGA CAACGAGCAG 
TTTGACAAGG AGATTGAAGA GTGGGACGCC CTGCAGGCTC GCAAGGCCCA GCAGGAGAAG 
CTGGCCTTGG CCACAGCGGC GGCGGCGGCC GCTGCCTCGG CAGCATCCAG CACCTCGACT 
TCTTCTTCTT CCCCTCAGCC TATGAGCATC GAGTCCAGCC CTCCAGACAA CGCAGTCCCC 
CAGCAGGACC CGGAGTACAT GGAGCAGCCG TCGCCCACAA ATGACTCCAA GCACCTGCAG 
GAGGACACTG AGAGGGCCAT CTCCAGGGCT GCAGCCGAAC AGGAGGAGAG GAGCCCCGAA 
GCGTTGCTAA ATGCTGCCAT CAAGCTGGAG TACGGTCGTC TTCTGAGATT CGCCCAAGAA 
GACACCCCGC CTGAGAACGA CTACCGTCTG CAGCACATCA TCGTCTACTT CATCCAAAAC 
CAGGCGCCCA AGAAGATCCT GGAGAGGACT CTGCTGACAC AGTTTGCAGA CAGGAACCTG 
GCTTTTGATG AGAGATGTAA GAGCATTATG AATGTGGCAC GTGCCAAACT GGACCTAATT 
AAGCCGGAGG AGGTCAACAT GGACGAATAT GAGATGTGGC ATCAGGACTA CAGGAATTTC 
CGTGAGACAA CCGTCTTCAT GATGACCGGC TTGGAGCTCT TCCAGAAAAC AAATTACGTG 
GAGGCTCTGA TGTATCTGAT CTACGCATAC CAGTACAACA AGGAGCTGCT GTTGAAGGGG 
CTGTACAGGG GGCACGACGA GGAGCTGCTG GGGCATTACC GCCGAGAGTG TCTGCTGAAA 
CTAAACGAGC AAGCGGCCGC CATGTTCGAG TCGGGAGAAG AACCTGAGGT GACCACGGGC 
CTGGGCATCA TGAACGAGCT GGTGGTGCCC TGCATCCCCC TGCTGCTGGT CCACGACACG 
GAGAGGGACC TGCTGGCGGT GGAGGACATG AGGAACCGCT GGTGCTCCTA CCTAGGTCAA 
GAGATGGAAT CCAACCTCCA GGAAAAGCTG ACGGACTTCC TCCCCAAGTT ACTGGACTGC 
TCTACGGAGA TCAAAAGCTT CCACGACCCT CCCAAGCTGC CCTCCTACTC CACCCTGGAG 
CTGTGCGAAC GATTCAGCCG CGTCATGGCC GCCCTCGGCA GGGTGCCCTC CGAGGGCAGA 
TGA