LOC107710620 gene cDNA ORF clone, Sinocyclocheilus rhinocerous

mRNA	Protein	Name
XM_016515437.1	XP_016370923.1	uncharacterized protein LOC107710620 isoform X1
XM_016515438.1	XP_016370924.1	uncharacterized protein LOC107710620 isoform X2
XM_016515440.1	XP_016370926.1	uncharacterized protein LOC107710620 isoform X4
XM_016515443.1	XP_016370929.1	uncharacterized protein LOC107710620 isoform X7
XM_016515445.1	XP_016370931.1	uncharacterized protein LOC107710620 isoform X9
XM_016515447.1	XP_016370933.1	rho guanine nucleotide exchange factor 4-like isoform X11
XM_016515448.1	XP_016370934.1	uncharacterized protein LOC107710620 isoform X12
XM_016515444.1	XP_016370930.1	uncharacterized protein LOC107710620 isoform X8
XM_016515446.1	XP_016370932.1	rho guanine nucleotide exchange factor 4-like isoform X10
XM_016515441.1	XP_016370927.1	uncharacterized protein LOC107710620 isoform X5
XM_016515439.1	XP_016370925.1	uncharacterized protein LOC107710620 isoform X3
XM_016515442.1	XP_016370928.1	uncharacterized protein LOC107710620 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSi145740	XM_016515446.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OSi145741	XM_016515447.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OSi145731	XM_016515437.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSi145732	XM_016515438.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSi145733	XM_016515439.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OSi145734	XM_016515440.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OSi145735	XM_016515441.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OSi145736	XM_016515442.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OSi145737	XM_016515443.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OSi145738	XM_016515444.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OSi145739	XM_016515445.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OSi145742	XM_016515448.1 Latest version!	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

LOC107710620 ( XM_016515446.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370932.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OSi145740
Clone ID Related Accession (Same CDS sequence)	XM_016515446.1
Accession Version	XM_016515446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	rho guanine nucleotide exchange factor 4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG TGGCTGCAGT 
GGTGGCAGTT CAGAGCAGTT ACCTGGCTCT GACTCAGACT GTAGCCCAGC TCTGAAACTG 
GCCGGAGGGG TTAGAGCAGT GCGAGGGGTC ATGTCCCATT ACTGGAGTCT GGAGAGCCTT 
CACTCCACCA ACGCCTTGGT GATAACTGAT GGACCGCAGG ATAAGTCTGG CCTTGCGGAT 
GAGGTGGGGA GTGAAGATGA CCTGTACAAT GAGTTTCGCA GCTCCTCCAA CCGATTCGGA 
CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGCGTG 
TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGACGACC AGGAGCTGGG CTTCAAAGCT 
GGAGACGTCA TCGAAGTAGT GGACGCCACA AACAAGGAGT GGTGGTGGGG CCGGGTGCTG 
GATAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC 
ATGGAGGAAT ACTTAGCCCA TCTGGACGGG GCCTCGGAGG GGGGTGGGGC CAGCATGGGG 
GACCCCTTAG GGCCCGGTCT GCCCTGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG 
ATCATGAGCA CAGAGAGGGA TTACATCAAA CACCTGAAGG ACATCTGTGA GGGCTATATA 
AAACAGTGTC GGAAGAGGAC AGATATGTTT ACTGAGGAGC AACTGCGCAC TATCTTTGGG 
AACATCGATG AGCTCTACCG TTTCCAGAAG AAGTTTCTCA AAGCCTTGGA GAAGAAATTC 
AACAAAGACC ATCCTCACCT CAGCGAGATT GGCTCCTGCT TCTTAGAGCA TCAAACAAAC 
TTTCAGATCT ACTCTGAATA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA 
CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCCT GTCGCCTGCT CCAGAAGATG 
ATCGACATTT CCTTGGATGG ATTCCTGCTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT 
CTGCAGCTGG CTGAACTGCT GAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTA 
GAAGCTGCCT TAAATGCAAT GAAGAATGTG GCCCGACTGA TCAATGAGAG AAAGAGGCGT 
CTGGAGAATA TTGACAAAAT TGCTCAGTGG CAAAGCTCCA TAGAAGACTG GGAGGGTGAA 
GACATCTTGA GCAGAAGCTC TGATCTGATT TTCTCAGGAG ACCTGACCAA GATCTCCCAG 
CCACAGGGCA AAGGCCAGCA GCGAATGTTC TTTCTCTTTG ACCACCAACT GGTTTTCTGT 
AAGAAGGATC TGCTTCGGAG GGACATCCTG TACTACAAGG GTCGGTTAGA CATGGATCAG 
ATGCAAGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAACGTGAG TGTGAAGAAT 
GCCTTGAAAT TATGTTCTCC TGTGGGAGAG GAGGTCCACC TTCTGTGTGC CAAGAAACCC 
GAGCAGAAGC AGCGCTGGCT GCGAGCCTTT GCGGATGAAC GGGAACAGGT CCAGCACGAC 
CTCGAAACAG GTTTTACGAT CACAGAGGTC CAGAAGAAGC AGGCCATGTT GAATGCATCT 
AAAAGTCATC CAACAGGAAA GCCCAAAGCT GTGACCAGGC CCTACTATGA CTTCCTGCTG 
CGTCAGAAGC ACCCCACGCT TCCCACCTCC CTGCCCCAGC AGCAGGTCAT CATGTTGGCC 
GAACCCAAAC GCAAGACCTC CAATTTCTGG CACAACATCG GACGCTTGAC ACCGTTCAAA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370932.1
CDS	263..2128
Translation	MRTQHSAGEVGVPLAGGGCSGGSSEQLPGSDSDCSPALKLAGGVRAVRGVMSHYWSLESLHSTNALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515446.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515446.1

ORF Insert Sequence:

ATGCGCACCC AGCACAGTGC CGGTGAGGTG GGCGTCCCGC TGGCAGGAGG TGGCTGCAGT 
GGTGGCAGTT CAGAGCAGTT ACCTGGCTCT GACTCAGACT GTAGCCCAGC TCTGAAACTG 
GCCGGAGGGG TTAGAGCAGT GCGAGGGGTC ATGTCCCATT ACTGGAGTCT GGAGAGCCTT 
CACTCCACCA ACGCCTTGGT GATAACTGAT GGACCGCAGG ATAAGTCTGG CCTTGCGGAT 
GAGGTGGGGA GTGAAGATGA CCTGTACAAT GAGTTTCGCA GCTCCTCCAA CCGATTCGGA 
CACCCAGGAG GAGGAGGAGA ACAGCTGGCC ATAAATGAGC TGATCAGTGA TGGCAGCGTG 
TGCGCGGAGG CTCTATGGGA CCACGTCACC ATGGACGACC AGGAGCTGGG CTTCAAAGCT 
GGAGACGTCA TCGAAGTAGT GGACGCCACA AACAAGGAGT GGTGGTGGGG CCGGGTGCTG 
GATAGCGAGG GCTGGTTCCC CGCCAGCTTT GTTCGGTTAC GGGTGAACCA AGATGAGCCC 
ATGGAGGAAT ACTTAGCCCA TCTGGACGGG GCCTCGGAGG GGGGTGGGGC CAGCATGGGG 
GACCCCTTAG GGCCCGGTCT GCCCTGCAAG GAGCAGATGA GAGCCAACGT CATCAATGAG 
ATCATGAGCA CAGAGAGGGA TTACATCAAA CACCTGAAGG ACATCTGTGA GGGCTATATA 
AAACAGTGTC GGAAGAGGAC AGATATGTTT ACTGAGGAGC AACTGCGCAC TATCTTTGGG 
AACATCGATG AGCTCTACCG TTTCCAGAAG AAGTTTCTCA AAGCCTTGGA GAAGAAATTC 
AACAAAGACC ATCCTCACCT CAGCGAGATT GGCTCCTGCT TCTTAGAGCA TCAAACAAAC 
TTTCAGATCT ACTCTGAATA TTGCAACAAC CACCCCAATG CCTGCGTGCA GCTCTCCAAA 
CTGATGAAGA TCAAGAAGTA TGTGTTCTTC TTTGAGGCCT GTCGCCTGCT CCAGAAGATG 
ATCGACATTT CCTTGGATGG ATTCCTGCTT ACTCCTGTTC AGAAGATCTG CAAGTATCCT 
CTGCAGCTGG CTGAACTGCT GAAGTACACC AACCCACAGC ACAGAGATTA TAAGGATGTA 
GAAGCTGCCT TAAATGCAAT GAAGAATGTG GCCCGACTGA TCAATGAGAG AAAGAGGCGT 
CTGGAGAATA TTGACAAAAT TGCTCAGTGG CAAAGCTCCA TAGAAGACTG GGAGGGTGAA 
GACATCTTGA GCAGAAGCTC TGATCTGATT TTCTCAGGAG ACCTGACCAA GATCTCCCAG 
CCACAGGGCA AAGGCCAGCA GCGAATGTTC TTTCTCTTTG ACCACCAACT GGTTTTCTGT 
AAGAAGGATC TGCTTCGGAG GGACATCCTG TACTACAAGG GTCGGTTAGA CATGGATCAG 
ATGCAAGTGG TGGATGTGGA GGATGGGAAG GATAAGGACT TTAACGTGAG TGTGAAGAAT 
GCCTTGAAAT TATGTTCTCC TGTGGGAGAG GAGGTCCACC TTCTGTGTGC CAAGAAACCC 
GAGCAGAAGC AGCGCTGGCT GCGAGCCTTT GCGGATGAAC GGGAACAGGT CCAGCACGAC 
CTCGAAACAG GTTTTACGAT CACAGAGGTC CAGAAGAAGC AGGCCATGTT GAATGCATCT 
AAAAGTCATC CAACAGGAAA GCCCAAAGCT GTGACCAGGC CCTACTATGA CTTCCTGCTG 
CGTCAGAAGC ACCCCACGCT TCCCACCTCC CTGCCCCAGC AGCAGGTCAT CATGTTGGCC 
GAACCCAAAC GCAAGACCTC CAATTTCTGG CACAACATCG GACGCTTGAC ACCGTTCAAA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515447.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370933.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OSi145741
Clone ID Related Accession (Same CDS sequence)	XM_016515447.1
Accession Version	XM_016515447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	rho guanine nucleotide exchange factor 4-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTTC AGGATGCAGA CATCACAGTG CAGCTGATCA GTGATGGCAG CGTGTGCGCG 
GAGGCTCTAT GGGACCACGT CACCATGGAC GACCAGGAGC TGGGCTTCAA AGCTGGAGAC 
GTCATCGAAG TAGTGGACGC CACAAACAAG GAGTGGTGGT GGGGCCGGGT GCTGGATAGC 
GAGGGCTGGT TCCCCGCCAG CTTTGTTCGG TTACGGGTGA ACCAAGATGA GCCCATGGAG 
GAATACTTAG CCCATCTGGA CGGGGCCTCG GAGGGGGGTG GGGCCAGCAT GGGGGACCCC 
TTAGGGCCCG GTCTGCCCTG CAAGGAGCAG ATGAGAGCCA ACGTCATCAA TGAGATCATG 
AGCACAGAGA GGGATTACAT CAAACACCTG AAGGACATCT GTGAGGGCTA TATAAAACAG 
TGTCGGAAGA GGACAGATAT GTTTACTGAG GAGCAACTGC GCACTATCTT TGGGAACATC 
GATGAGCTCT ACCGTTTCCA GAAGAAGTTT CTCAAAGCCT TGGAGAAGAA ATTCAACAAA 
GACCATCCTC ACCTCAGCGA GATTGGCTCC TGCTTCTTAG AGCATCAAAC AAACTTTCAG 
ATCTACTCTG AATATTGCAA CAACCACCCC AATGCCTGCG TGCAGCTCTC CAAACTGATG 
AAGATCAAGA AGTATGTGTT CTTCTTTGAG GCCTGTCGCC TGCTCCAGAA GATGATCGAC 
ATTTCCTTGG ATGGATTCCT GCTTACTCCT GTTCAGAAGA TCTGCAAGTA TCCTCTGCAG 
CTGGCTGAAC TGCTGAAGTA CACCAACCCA CAGCACAGAG ATTATAAGGA TGTAGAAGCT 
GCCTTAAATG CAATGAAGAA TGTGGCCCGA CTGATCAATG AGAGAAAGAG GCGTCTGGAG 
AATATTGACA AAATTGCTCA GTGGCAAAGC TCCATAGAAG ACTGGGAGGG TGAAGACATC 
TTGAGCAGAA GCTCTGATCT GATTTTCTCA GGAGACCTGA CCAAGATCTC CCAGCCACAG 
GGCAAAGGCC AGCAGCGAAT GTTCTTTCTC TTTGACCACC AACTGGTTTT CTGTAAGAAG 
GATCTGCTTC GGAGGGACAT CCTGTACTAC AAGGGTCGGT TAGACATGGA TCAGATGCAA 
GTGGTGGATG TGGAGGATGG GAAGGATAAG GACTTTAACG TGAGTGTGAA GAATGCCTTG 
AAATTATGTT CTCCTGTGGG AGAGGAGGTC CACCTTCTGT GTGCCAAGAA ACCCGAGCAG 
AAGCAGCGCT GGCTGCGAGC CTTTGCGGAT GAACGGGAAC AGGTCCAGCA CGACCTCGAA 
ACAGGTTTTA CGATCACAGA GGTCCAGAAG AAGCAGGCCA TGTTGAATGC ATCTAAAAGT 
CATCCAACAG GAAAGCCCAA AGCTGTGACC AGGCCCTACT ATGACTTCCT GCTGCGTCAG 
AAGCACCCCA CGCTTCCCAC CTCCCTGCCC CAGCAGCAGG TCATCATGTT GGCCGAACCC 
AAACGCAAGA CCTCCAATTT CTGGCACAAC ATCGGACGCT TGACACCGTT CAAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370933.1
CDS	1478..3037
Translation	MGFQDADITVQLISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515447.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515447.1

ORF Insert Sequence:

ATGGGTTTTC AGGATGCAGA CATCACAGTG CAGCTGATCA GTGATGGCAG CGTGTGCGCG 
GAGGCTCTAT GGGACCACGT CACCATGGAC GACCAGGAGC TGGGCTTCAA AGCTGGAGAC 
GTCATCGAAG TAGTGGACGC CACAAACAAG GAGTGGTGGT GGGGCCGGGT GCTGGATAGC 
GAGGGCTGGT TCCCCGCCAG CTTTGTTCGG TTACGGGTGA ACCAAGATGA GCCCATGGAG 
GAATACTTAG CCCATCTGGA CGGGGCCTCG GAGGGGGGTG GGGCCAGCAT GGGGGACCCC 
TTAGGGCCCG GTCTGCCCTG CAAGGAGCAG ATGAGAGCCA ACGTCATCAA TGAGATCATG 
AGCACAGAGA GGGATTACAT CAAACACCTG AAGGACATCT GTGAGGGCTA TATAAAACAG 
TGTCGGAAGA GGACAGATAT GTTTACTGAG GAGCAACTGC GCACTATCTT TGGGAACATC 
GATGAGCTCT ACCGTTTCCA GAAGAAGTTT CTCAAAGCCT TGGAGAAGAA ATTCAACAAA 
GACCATCCTC ACCTCAGCGA GATTGGCTCC TGCTTCTTAG AGCATCAAAC AAACTTTCAG 
ATCTACTCTG AATATTGCAA CAACCACCCC AATGCCTGCG TGCAGCTCTC CAAACTGATG 
AAGATCAAGA AGTATGTGTT CTTCTTTGAG GCCTGTCGCC TGCTCCAGAA GATGATCGAC 
ATTTCCTTGG ATGGATTCCT GCTTACTCCT GTTCAGAAGA TCTGCAAGTA TCCTCTGCAG 
CTGGCTGAAC TGCTGAAGTA CACCAACCCA CAGCACAGAG ATTATAAGGA TGTAGAAGCT 
GCCTTAAATG CAATGAAGAA TGTGGCCCGA CTGATCAATG AGAGAAAGAG GCGTCTGGAG 
AATATTGACA AAATTGCTCA GTGGCAAAGC TCCATAGAAG ACTGGGAGGG TGAAGACATC 
TTGAGCAGAA GCTCTGATCT GATTTTCTCA GGAGACCTGA CCAAGATCTC CCAGCCACAG 
GGCAAAGGCC AGCAGCGAAT GTTCTTTCTC TTTGACCACC AACTGGTTTT CTGTAAGAAG 
GATCTGCTTC GGAGGGACAT CCTGTACTAC AAGGGTCGGT TAGACATGGA TCAGATGCAA 
GTGGTGGATG TGGAGGATGG GAAGGATAAG GACTTTAACG TGAGTGTGAA GAATGCCTTG 
AAATTATGTT CTCCTGTGGG AGAGGAGGTC CACCTTCTGT GTGCCAAGAA ACCCGAGCAG 
AAGCAGCGCT GGCTGCGAGC CTTTGCGGAT GAACGGGAAC AGGTCCAGCA CGACCTCGAA 
ACAGGTTTTA CGATCACAGA GGTCCAGAAG AAGCAGGCCA TGTTGAATGC ATCTAAAAGT 
CATCCAACAG GAAAGCCCAA AGCTGTGACC AGGCCCTACT ATGACTTCCT GCTGCGTCAG 
AAGCACCCCA CGCTTCCCAC CTCCCTGCCC CAGCAGCAGG TCATCATGTT GGCCGAACCC 
AAACGCAAGA CCTCCAATTT CTGGCACAAC ATCGGACGCT TGACACCGTT CAAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515437.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370923.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSi145731
Clone ID Related Accession (Same CDS sequence)	XM_016515437.1
Accession Version	XM_016515437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
CTGATCAGTG ATGGCAGCGT GTGCGCGGAG GCTCTATGGG ACCACGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGAGACGTC ATCGAAGTAG TGGACGCCAC AAACAAGGAG 
TGGTGGTGGG GCCGGGTGCT GGATAGCGAG GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA 
CGGGTGAACC AAGATGAGCC CATGGAGGAA TACTTAGCCC ATCTGGACGG GGCCTCGGAG 
GGGGGTGGGG CCAGCATGGG GGACCCCTTA GGGCCCGGTC TGCCCTGCAA GGAGCAGATG 
AGAGCCAACG TCATCAATGA GATCATGAGC ACAGAGAGGG ATTACATCAA ACACCTGAAG 
GACATCTGTG AGGGCTATAT AAAACAGTGT CGGAAGAGGA CAGATATGTT TACTGAGGAG 
CAACTGCGCA CTATCTTTGG GAACATCGAT GAGCTCTACC GTTTCCAGAA GAAGTTTCTC 
AAAGCCTTGG AGAAGAAATT CAACAAAGAC CATCCTCACC TCAGCGAGAT TGGCTCCTGC 
TTCTTAGAGC ATCAAACAAA CTTTCAGATC TACTCTGAAT ATTGCAACAA CCACCCCAAT 
GCCTGCGTGC AGCTCTCCAA ACTGATGAAG ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC 
TGTCGCCTGC TCCAGAAGAT GATCGACATT TCCTTGGATG GATTCCTGCT TACTCCTGTT 
CAGAAGATCT GCAAGTATCC TCTGCAGCTG GCTGAACTGC TGAAGTACAC CAACCCACAG 
CACAGAGATT ATAAGGATGT AGAAGCTGCC TTAAATGCAA TGAAGAATGT GGCCCGACTG 
ATCAATGAGA GAAAGAGGCG TCTGGAGAAT ATTGACAAAA TTGCTCAGTG GCAAAGCTCC 
ATAGAAGACT GGGAGGGTGA AGACATCTTG AGCAGAAGCT CTGATCTGAT TTTCTCAGGA 
GACCTGACCA AGATCTCCCA GCCACAGGGC AAAGGCCAGC AGCGAATGTT CTTTCTCTTT 
GACCACCAAC TGGTTTTCTG TAAGAAGGAT CTGCTTCGGA GGGACATCCT GTACTACAAG 
GGTCGGTTAG ACATGGATCA GATGCAAGTG GTGGATGTGG AGGATGGGAA GGATAAGGAC 
TTTAACGTGA GTGTGAAGAA TGCCTTGAAA TTATGTTCTC CTGTGGGAGA GGAGGTCCAC 
CTTCTGTGTG CCAAGAAACC CGAGCAGAAG CAGCGCTGGC TGCGAGCCTT TGCGGATGAA 
CGGGAACAGG TCCAGCACGA CCTCGAAACA GGTTTTACGA TCACAGAGGT CCAGAAGAAG 
CAGGCCATGT TGAATGCATC TAAAAGTCAT CCAACAGGAA AGCCCAAAGC TGTGACCAGG 
CCCTACTATG ACTTCCTGCT GCGTCAGAAG CACCCCACGC TTCCCACCTC CCTGCCCCAG 
CAGCAGGTCA TCATGTTGGC CGAACCCAAA CGCAAGACCT CCAATTTCTG GCACAACATC 
GGACGCTTGA CACCGTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370923.1
CDS	431..8497
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQRLRPSPVLPQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515437.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515437.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
CTGATCAGTG ATGGCAGCGT GTGCGCGGAG GCTCTATGGG ACCACGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGAGACGTC ATCGAAGTAG TGGACGCCAC AAACAAGGAG 
TGGTGGTGGG GCCGGGTGCT GGATAGCGAG GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA 
CGGGTGAACC AAGATGAGCC CATGGAGGAA TACTTAGCCC ATCTGGACGG GGCCTCGGAG 
GGGGGTGGGG CCAGCATGGG GGACCCCTTA GGGCCCGGTC TGCCCTGCAA GGAGCAGATG 
AGAGCCAACG TCATCAATGA GATCATGAGC ACAGAGAGGG ATTACATCAA ACACCTGAAG 
GACATCTGTG AGGGCTATAT AAAACAGTGT CGGAAGAGGA CAGATATGTT TACTGAGGAG 
CAACTGCGCA CTATCTTTGG GAACATCGAT GAGCTCTACC GTTTCCAGAA GAAGTTTCTC 
AAAGCCTTGG AGAAGAAATT CAACAAAGAC CATCCTCACC TCAGCGAGAT TGGCTCCTGC 
TTCTTAGAGC ATCAAACAAA CTTTCAGATC TACTCTGAAT ATTGCAACAA CCACCCCAAT 
GCCTGCGTGC AGCTCTCCAA ACTGATGAAG ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC 
TGTCGCCTGC TCCAGAAGAT GATCGACATT TCCTTGGATG GATTCCTGCT TACTCCTGTT 
CAGAAGATCT GCAAGTATCC TCTGCAGCTG GCTGAACTGC TGAAGTACAC CAACCCACAG 
CACAGAGATT ATAAGGATGT AGAAGCTGCC TTAAATGCAA TGAAGAATGT GGCCCGACTG 
ATCAATGAGA GAAAGAGGCG TCTGGAGAAT ATTGACAAAA TTGCTCAGTG GCAAAGCTCC 
ATAGAAGACT GGGAGGGTGA AGACATCTTG AGCAGAAGCT CTGATCTGAT TTTCTCAGGA 
GACCTGACCA AGATCTCCCA GCCACAGGGC AAAGGCCAGC AGCGAATGTT CTTTCTCTTT 
GACCACCAAC TGGTTTTCTG TAAGAAGGAT CTGCTTCGGA GGGACATCCT GTACTACAAG 
GGTCGGTTAG ACATGGATCA GATGCAAGTG GTGGATGTGG AGGATGGGAA GGATAAGGAC 
TTTAACGTGA GTGTGAAGAA TGCCTTGAAA TTATGTTCTC CTGTGGGAGA GGAGGTCCAC 
CTTCTGTGTG CCAAGAAACC CGAGCAGAAG CAGCGCTGGC TGCGAGCCTT TGCGGATGAA 
CGGGAACAGG TCCAGCACGA CCTCGAAACA GGTTTTACGA TCACAGAGGT CCAGAAGAAG 
CAGGCCATGT TGAATGCATC TAAAAGTCAT CCAACAGGAA AGCCCAAAGC TGTGACCAGG 
CCCTACTATG ACTTCCTGCT GCGTCAGAAG CACCCCACGC TTCCCACCTC CCTGCCCCAG 
CAGCAGGTCA TCATGTTGGC CGAACCCAAA CGCAAGACCT CCAATTTCTG GCACAACATC 
GGACGCTTGA CACCGTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515438.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370924.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X2, mRNA.

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CloneID	OSi145732
Clone ID Related Accession (Same CDS sequence)	XM_016515438.1
Accession Version	XM_016515438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
GCCCCAGCTT TAGAGGACTC TGGGAGTGAG GCAGCCTGTC TGGATGATGG GGAGTCTCTG 
CAGGAGGGGC ATGGAGAGCA ACAGGACTAT CAGGAATGGT GTGAGCCAGT TTCTGAAGCA 
GCACAGGGCA CAAAAGAGGA ATATTTTGAC ACACACTCAT GGCACAGTGA CACTTTCTCT 
GGCTTGAGTC ATGAAACTAA GGACTGCTGC AGGACACCGC TCAGCTCTGT GGGTCATCTG 
GACTCACACT CACCTTCGGT TAGAAGGAGA GATCCCTCTT CATCAAAACT GCATCATTCT 
GTCAATCATG CTGACAGTCT CAGTAGAACA GAGGATAGAG AACAATTGCA AAAGCAGACT 
GAAAATATGG GGATGATGTG CATGGGAGTT GATGGCACTG AAATGTCTCA AGCTAAGGGT 
GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT TTTCCAGCGG GACCTGATGT TGATTCTTCT 
TCAAGGAATG ACCAATCCTA CATCGTAACA CCCCTACTGG CATTAGGGAA CGCTGTAGAA 
CCACAAACAC TCACTGAGCC ATGTGCTAAT GACTCATTAT TGACCTTAAC AGACTGTGAT 
GGAGCCAAGT CTCAGTTTGC TGACACCTTT GAGAGAAGAT CAAAATGGGA TCAAATTAAC 
AAAAATATCA CAGACACTCA GTCAGGACAC AATGAGCCGC GAATACAGTT CATAACCTCA 
AGTGAGAAGC AGTTACAGCA AACTTATATT GACAGTAGTA ACACTGAAGT ACAGTTGATT 
TCGAATGCAT CTGTTGCGAA TAGTAAAGAT AACGAATGTC GTGAGCTGGG TGTTGATGAA 
AATAAGATCA GTTTATCTGT ATCACGTCAA CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG 
AAGCCTATTG AACAGGAATT TGAGGAGTCA ACAAAACACA GCCAATCACA GCCCTCAGTA 
GAGCACAGCA AATCACAGCC CTCAGTAGAG CACAGCAAAT CACAGCCCTC AGTAGAGCAC 
AGCCAATCAC AGGCATCAAC AGAACAAATC CAATCACAGG ATTCTGTAGA ATGCAGCCAA 
TCACGGAATT CAGCAGAACA CAGGCAATCA CGAGACTCAA TAGAACACAG TCAGTCACAG 
GATTCAGTAG AATGTAGGCA ATCACAGAAT TCAGTAGAAG ACAGCCAACT ACAAGACTCA 
GTAGAACATA GTCAGCCAAA GGATTTTGTA GAATGCAGCC AATCACATGA TTCTATAGAA 
TGCAGCCACT CACTGTATTC AGTAAAACAC AGGCAATCAC AAGACTCAAT AGAACACGGC 
CAGTCACAGA GCTCAGTAGA ATACACCAAA TCACAGGATT CTGTAAAATG CAGTCAATCA 
CAGAATTCAG TAGAACACAA CCAATCACAG ACCCCAGTAG AACACACCAA ATCACAGGAT 
TCTGTAAAAT GCAGTCAATC ACAGAATTCA GCAGAACACA ACCAATCACA GGACTCAGTA 
GAACACAGTC AGTCACAGAG CTCAGAAGAC ATGCCATCCC AAAATTTCAT CAAACACAGC 
CAATCACAGA CCCCAGTAGA ACACCCCAAA TCACAGGATT GTGTAAAATG CAGTCAATCA 
CAGAATTCAG TAGAACACAA CCAATCACGG GACTCAGTAG AACACAGCCA GTCACACAGC 
TCAGTAGAAG ACATGCTATC GCAAAATGTA ATTAACCACA ACAAATCACA AGACCCAGTA 
GAATATACTC TGTCACTGAC CTCACTACAA CACACCCAAT CACAGGATTC TATAGAACTT 
AGCCCATCAA ATGATTCTGT AGAATGCAGC CAATCACAGG ATTCAGTAGA AAACACATTA 
GAAAGTAGAA AACGTCAGTC ACATACTACA TCAGAACACA TTCAGTTACA GAAGTCTATA 
GAATGTAGCC AATCACAGAA TTCCATACAA CACAGGAAAT CACAGGATTT AGCAGAACAA 
AACCAATCAC AGAACACCAT AGAGCACAGC CAATCACAAA CTCCAGTGGA ACACAGCCAA 
TCACAAACTT CAGTGCAACA CAGCCAATCA CAAACTTCAG TGGAACACAG CCAATCACAA 
GTTGATTCCA TTAAGGCAAA TTCTATGAAT ACCCAGTGGA CTGTGACCGA GTCAGACTCT 
GGTTTTATTT CAAACACAGC TTCTCTTTAT GAAAAGGGAC AATTAAAACA GGACTCCGTG 
GACGTATGCA TGAATTGTGA AGTCAGTCAG TCCTTGAAAA CATCTGGAAG GCTTTCATCA 
AAGGATACTG ACAAAGAACT CGATTTGAAC TTCGAAAAGA ATAACAGTCT AAATTTAAAA 
AAGCAGAACT TAAATGACAC CCCAAGTGTT GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG 
AGAGCTTTCT CAAATGTGCC TAATGCAACT TCTGCTGGAG TGTCAGATTC ACTGAATCAG 
CATGTGATCA ATGACCGGAC CTCAGAACAG TCAGATCTGC AGGCCAGACC TCATTACGGT 
CAACAAAATT TCCCAGTTTT ACAAATGCAC ACTGAAAAAA AGGATTACTC AGTTAGTAGT 
TATCACATGG ACAGTCATTG CTTAAAGGAC ATTGTTTTAG GGGAACATGA GCCTGAGGCA 
GATTGTTATG AAACCCGTCA GTGCCGTAAT AGCAGAATTA ACTGTAACAC ACAGGGTGAA 
TTCACTGAAG CACAAGTGTA TGTTAACCCT AAAACGTTTG ATACCAATGA TGGTGACGTT 
TTAGAAGGAA CACAGAAAAA TAGCAAAACA CATCACATCA TTAAAGGGTA CCAGAAAGAA 
TGTACAATAG TTCCCTCGAT AGAACAGTCA GTAATTTACT CTGATAGTTC GGGATGCATA 
GAATACACTA GGCCTAAGTC ATTGAGTGTC ACGTGTCCTG ACATGAAGAC AGCTGAAGCC 
GATGGGTGTC CTGTAGACAG TCCTGTCGAA TCTCTGGCTA GTCTGTTTGA GATTTCGTAT 
GACAAATCTC AAGTCAGCCC CACAGATGTT TGCTTTGAGC TCAGTGATGG CCTTCATATG 
AACTGTAAAC AAGTAGCACA TTGCATTATA GAATATACAG AGACCTCCAA ACAATTTCAC 
AGACCCGACA CCCTGTCTGA CAAACAAGCA AGACAAAACG AGTCAGTTGA AACAATTGAG 
CCGGATATTG ATATTTTGTC TAGTGCATGT ACTGATCCTT TCACAAATGC ACAATCCCCT 
TTTGATTATT CCCAGCTGAA CAGTAGAAAC AATGAATACA GCTCAGACAC AAATAATAAT 
ATTAAAGCCA TTACTTTTGA AGAGTGTGCC TCAGGTGAAC AGATCTCTTT GCCCAATTCT 
TTGCAAAAAT ACAGCAGGTT TAGTATCAAT GCTGGATCTT TACTCTCAAA AGGTGGTGAT 
TGCAAAGACA ATGCCCCTCC TAGTCAGGCT ACTCCCTTTA AAGCACCTGA AATGAGGACT 
GCAATTGACA GCAAAGATAA TGTTGCTAAA CCTTCTGAAT TCAATGAATT CAGCAGCCAT 
AGAGATGCTA TACTAAAGCA GCTCTGTGAT TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC 
TCGTCTGCAG TGGAAGGAGA GCAGACAGCA GGAGGATTTC AGTTCAAGGG CACAGACGGC 
ATCAGTCAGA GCAGAACTGC AGTGTTGCAA ACCTTGAGTC CCTCACACAC ACATGACCAA 
TGCAACACAC ATGGAGAGGT TAATGCAGAC TCTACAAACT GTGAATTTTC ACATATTGGC 
CATTTGCTTG CACACAAACA TCCGGAGCCC GTCCAAGAGA AAAGCACATT ACCACTGAGT 
GGCTCACTCA AGGTCGATGA TACTTATGCA TTGGAAAACA TGGAGAGAAA GCTTGAAACT 
TTCATGGCTA GCTCTCCTGA AAGCACTGAT TCAATATGTG AAACTTCTGA TCCTCTATGT 
AAGCAACCTT TGCATCTGCG CAACTCGGAG ACCGAGGGTG CATTTGTTTC ATGTTGCCGT 
ATTTTACCCA TCACTAATTT AGAGGCCATT TTGGAGTTGG ACCGTTCACC GGAGAGCTCA 
TTTGTGATAG AAAATGGACT GGGGGAGCAA CAGGGAGGAA GTCCATTATC AGAAGGTGGA 
TTCAGTGAGG AATGGAGCTC TTGTGATGAC GTCACAAACC GCCCACATGA GAACATTTCT 
GCCATGTGTA CAAAAGCCAA CGTAGCCACT GTCATGAATG AACAAGATGC TGGAGAAACA 
ATGAGCTCTT TATCAAATGG TTTTGATAGT GATGGTTATA GTGTTTCCAG TTTTGTGTCA 
CAAGACCTTG ACTCCAGTAC CAACAGTGAA GAGACGGAGG ACAGCACTCA GTCATCATCT 
AGTCTTCGTG GTTCAAGAAA TAAGAGAACC CAATCAGGGA AGGGTTCTGT GTTTTCTGTT 
TTTTCTCGAA TGCCCTCATT CCGCAAGATT AAACGAGAAC ACAAAGGCAA TAGCAAGGTT 
GATCCAGAAA TTAAAGATTC CAATGAGGAT GGAGATGAAC GGGGTGAGTT GAAGTACAAT 
AGGTCAACCA CACATAACAA ATCCAACCCT TCACTGTACA ATACCTCTTT TTCTCAAAGT 
ACAGATCATC TTACAGACAT TACAAAGTTT GTTGACCACT CCAATGATGA TATTTTTGAA 
AAAGCTTTTG CTTTAAACAT GCAGAATAGA GAGAAGTATA CACAATCCCA AAATTTGTAT 
CAGCAGCACA AAGATGGAGA TGCACTGAAT TTTAGAGTTT CCCCAACGAC AGAGGGATTG 
CAACAAAAAA GAAGCAAGAG TACTGATAAC TTAAACTTGC GCATGAAGCT CGCAATGGCT 
CACAAGTCTC TGTCTAGCCT GTTTGAGTCC AGATATCCTG AGAGGGACAA CCAGGAGCAG 
ATTGCACAGT CAGAGAATGA GGAGGTAAAA ACCAAGCAGT CCTGGAGGAA GTCTAAACGG 
TCTAAGGAAG CAGAACTTTG CAAAAGAACT CTATCGGTTC CTGGGATGGT CTGTGACAAG 
TCCATACATC AGAGCCATAT CGATTGTGCC TTCTGTGCTC CTCAGAACAG GTCCAGACTT 
AATAGCTCAC CAGCTTCCCA AAAGAGTTTG AGGAGCACGT GCCATTCTGA TTCACAGAGT 
TTGAAATCTG GTCTTCAGGA ACAGTCTGAA GACAGAAAAT ATCTGGAAGG AGGTGAGCTG 
CCTTGTGTGC CTTACTTAGA TTCTGACCAG ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA 
GAGGATAGGT CGCAATCACC TGTACACCAA ACTGTTTTTG CACTTGCAAA CCAGATGTCA 
CCTACCTGGA CCAGATCTTT AAGCTGTTTT GAGACCACTG ATACGCCAAC GAGGCCCATG 
AGCCCTAAAC CTCATAGTCC TGGTCTGGGC TGGACACACC GGAGGAGCTT CCGTTACCCT 
TCGAGATCTG TTGCATCTTC TCTCTGCTCA CTCGGTCAAG GAGTGAGCAC TGACGGTCTC 
TCCGATCCAC CTCAGAGACC GACGTCTCTG AAACCAAGGA CAGCACAACT TACAACAACC 
CACTCTTTTG ATTCTGAGTC CTTACTAGAG GACAGCAGTT CAGACAGCCA GTCTCAAAAT 
AGCCTGAATT CAGCATCCCT GATCAACAAA CCAGAGCATG TACAGCACAG TACTGAGCCA 
GCCATCCAGT CAGACGAGAG AAGACAACAG GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC 
AGACAGCAGG GTAAAGCTGC ACCCAGACCC ATCTCAGACC TCTACGGTTG GACCGTGTCT 
CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT CTGGAGAGGA AGAGCAATTC GACCGAAACC 
CGTCGGCGTT CATGCTCATA TGAAACGTTG ACTGATGTGG AGAACACCCA TAAAAAGAAG 
CTTAACACGC GGCAAAGTTT GCGTCAGCTT AGCTCACCAA CATCTCAGAA GCACGAGAAA 
GTGTGCGCTA GACTTTCCCT CACTTCTCCA GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC 
CACTTCTTCT CCCAGAGCAC ACCTGTTGGA CTGGACTTTT TGGGCTGGCC TCACCGTGCA 
TCATTTTCAG AATCGAATCT CAGCACACCA GCACAAGAGG CACATGAACA GGACCCTCCT 
CTGGCCTTGG TGATAACTGA TGGACCGCAG GATAAGTCTG GCCTTGCGGA TGAGGTGGGG 
AGTGAAGATG ACCTGTACAA TGAGTTTCGC AGCTCCTCCA ACCGATTCGG ACACCCAGGA 
GGAGGAGGAG AACAGCTGGC CATAAATGAG CTGATCAGTG ATGGCAGCGT GTGCGCGGAG 
GCTCTATGGG ACCACGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGAGACGTC 
ATCGAAGTAG TGGACGCCAC AAACAAGGAG TGGTGGTGGG GCCGGGTGCT GGATAGCGAG 
GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA CGGGTGAACC AAGATGAGCC CATGGAGGAA 
TACTTAGCCC ATCTGGACGG GGCCTCGGAG GGGGGTGGGG CCAGCATGGG GGACCCCTTA 
GGGCCCGGTC TGCCCTGCAA GGAGCAGATG AGAGCCAACG TCATCAATGA GATCATGAGC 
ACAGAGAGGG ATTACATCAA ACACCTGAAG GACATCTGTG AGGGCTATAT AAAACAGTGT 
CGGAAGAGGA CAGATATGTT TACTGAGGAG CAACTGCGCA CTATCTTTGG GAACATCGAT 
GAGCTCTACC GTTTCCAGAA GAAGTTTCTC AAAGCCTTGG AGAAGAAATT CAACAAAGAC 
CATCCTCACC TCAGCGAGAT TGGCTCCTGC TTCTTAGAGC ATCAAACAAA CTTTCAGATC 
TACTCTGAAT ATTGCAACAA CCACCCCAAT GCCTGCGTGC AGCTCTCCAA ACTGATGAAG 
ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC TGTCGCCTGC TCCAGAAGAT GATCGACATT 
TCCTTGGATG GATTCCTGCT TACTCCTGTT CAGAAGATCT GCAAGTATCC TCTGCAGCTG 
GCTGAACTGC TGAAGTACAC CAACCCACAG CACAGAGATT ATAAGGATGT AGAAGCTGCC 
TTAAATGCAA TGAAGAATGT GGCCCGACTG ATCAATGAGA GAAAGAGGCG TCTGGAGAAT 
ATTGACAAAA TTGCTCAGTG GCAAAGCTCC ATAGAAGACT GGGAGGGTGA AGACATCTTG 
AGCAGAAGCT CTGATCTGAT TTTCTCAGGA GACCTGACCA AGATCTCCCA GCCACAGGGC 
AAAGGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAAC TGGTTTTCTG TAAGAAGGAT 
CTGCTTCGGA GGGACATCCT GTACTACAAG GGTCGGTTAG ACATGGATCA GATGCAAGTG 
GTGGATGTGG AGGATGGGAA GGATAAGGAC TTTAACGTGA GTGTGAAGAA TGCCTTGAAA 
TTATGTTCTC CTGTGGGAGA GGAGGTCCAC CTTCTGTGTG CCAAGAAACC CGAGCAGAAG 
CAGCGCTGGC TGCGAGCCTT TGCGGATGAA CGGGAACAGG TCCAGCACGA CCTCGAAACA 
GGTTTTACGA TCACAGAGGT CCAGAAGAAG CAGGCCATGT TGAATGCATC TAAAAGTCAT 
CCAACAGGAA AGCCCAAAGC TGTGACCAGG CCCTACTATG ACTTCCTGCT GCGTCAGAAG 
CACCCCACGC TTCCCACCTC CCTGCCCCAG CAGCAGGTCA TCATGTTGGC CGAACCCAAA 
CGCAAGACCT CCAATTTCTG GCACAACATC GGACGCTTGA CACCGTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370924.1
CDS	431..8467
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515438.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515438.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
GCCCCAGCTT TAGAGGACTC TGGGAGTGAG GCAGCCTGTC TGGATGATGG GGAGTCTCTG 
CAGGAGGGGC ATGGAGAGCA ACAGGACTAT CAGGAATGGT GTGAGCCAGT TTCTGAAGCA 
GCACAGGGCA CAAAAGAGGA ATATTTTGAC ACACACTCAT GGCACAGTGA CACTTTCTCT 
GGCTTGAGTC ATGAAACTAA GGACTGCTGC AGGACACCGC TCAGCTCTGT GGGTCATCTG 
GACTCACACT CACCTTCGGT TAGAAGGAGA GATCCCTCTT CATCAAAACT GCATCATTCT 
GTCAATCATG CTGACAGTCT CAGTAGAACA GAGGATAGAG AACAATTGCA AAAGCAGACT 
GAAAATATGG GGATGATGTG CATGGGAGTT GATGGCACTG AAATGTCTCA AGCTAAGGGT 
GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT TTTCCAGCGG GACCTGATGT TGATTCTTCT 
TCAAGGAATG ACCAATCCTA CATCGTAACA CCCCTACTGG CATTAGGGAA CGCTGTAGAA 
CCACAAACAC TCACTGAGCC ATGTGCTAAT GACTCATTAT TGACCTTAAC AGACTGTGAT 
GGAGCCAAGT CTCAGTTTGC TGACACCTTT GAGAGAAGAT CAAAATGGGA TCAAATTAAC 
AAAAATATCA CAGACACTCA GTCAGGACAC AATGAGCCGC GAATACAGTT CATAACCTCA 
AGTGAGAAGC AGTTACAGCA AACTTATATT GACAGTAGTA ACACTGAAGT ACAGTTGATT 
TCGAATGCAT CTGTTGCGAA TAGTAAAGAT AACGAATGTC GTGAGCTGGG TGTTGATGAA 
AATAAGATCA GTTTATCTGT ATCACGTCAA CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG 
AAGCCTATTG AACAGGAATT TGAGGAGTCA ACAAAACACA GCCAATCACA GCCCTCAGTA 
GAGCACAGCA AATCACAGCC CTCAGTAGAG CACAGCAAAT CACAGCCCTC AGTAGAGCAC 
AGCCAATCAC AGGCATCAAC AGAACAAATC CAATCACAGG ATTCTGTAGA ATGCAGCCAA 
TCACGGAATT CAGCAGAACA CAGGCAATCA CGAGACTCAA TAGAACACAG TCAGTCACAG 
GATTCAGTAG AATGTAGGCA ATCACAGAAT TCAGTAGAAG ACAGCCAACT ACAAGACTCA 
GTAGAACATA GTCAGCCAAA GGATTTTGTA GAATGCAGCC AATCACATGA TTCTATAGAA 
TGCAGCCACT CACTGTATTC AGTAAAACAC AGGCAATCAC AAGACTCAAT AGAACACGGC 
CAGTCACAGA GCTCAGTAGA ATACACCAAA TCACAGGATT CTGTAAAATG CAGTCAATCA 
CAGAATTCAG TAGAACACAA CCAATCACAG ACCCCAGTAG AACACACCAA ATCACAGGAT 
TCTGTAAAAT GCAGTCAATC ACAGAATTCA GCAGAACACA ACCAATCACA GGACTCAGTA 
GAACACAGTC AGTCACAGAG CTCAGAAGAC ATGCCATCCC AAAATTTCAT CAAACACAGC 
CAATCACAGA CCCCAGTAGA ACACCCCAAA TCACAGGATT GTGTAAAATG CAGTCAATCA 
CAGAATTCAG TAGAACACAA CCAATCACGG GACTCAGTAG AACACAGCCA GTCACACAGC 
TCAGTAGAAG ACATGCTATC GCAAAATGTA ATTAACCACA ACAAATCACA AGACCCAGTA 
GAATATACTC TGTCACTGAC CTCACTACAA CACACCCAAT CACAGGATTC TATAGAACTT 
AGCCCATCAA ATGATTCTGT AGAATGCAGC CAATCACAGG ATTCAGTAGA AAACACATTA 
GAAAGTAGAA AACGTCAGTC ACATACTACA TCAGAACACA TTCAGTTACA GAAGTCTATA 
GAATGTAGCC AATCACAGAA TTCCATACAA CACAGGAAAT CACAGGATTT AGCAGAACAA 
AACCAATCAC AGAACACCAT AGAGCACAGC CAATCACAAA CTCCAGTGGA ACACAGCCAA 
TCACAAACTT CAGTGCAACA CAGCCAATCA CAAACTTCAG TGGAACACAG CCAATCACAA 
GTTGATTCCA TTAAGGCAAA TTCTATGAAT ACCCAGTGGA CTGTGACCGA GTCAGACTCT 
GGTTTTATTT CAAACACAGC TTCTCTTTAT GAAAAGGGAC AATTAAAACA GGACTCCGTG 
GACGTATGCA TGAATTGTGA AGTCAGTCAG TCCTTGAAAA CATCTGGAAG GCTTTCATCA 
AAGGATACTG ACAAAGAACT CGATTTGAAC TTCGAAAAGA ATAACAGTCT AAATTTAAAA 
AAGCAGAACT TAAATGACAC CCCAAGTGTT GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG 
AGAGCTTTCT CAAATGTGCC TAATGCAACT TCTGCTGGAG TGTCAGATTC ACTGAATCAG 
CATGTGATCA ATGACCGGAC CTCAGAACAG TCAGATCTGC AGGCCAGACC TCATTACGGT 
CAACAAAATT TCCCAGTTTT ACAAATGCAC ACTGAAAAAA AGGATTACTC AGTTAGTAGT 
TATCACATGG ACAGTCATTG CTTAAAGGAC ATTGTTTTAG GGGAACATGA GCCTGAGGCA 
GATTGTTATG AAACCCGTCA GTGCCGTAAT AGCAGAATTA ACTGTAACAC ACAGGGTGAA 
TTCACTGAAG CACAAGTGTA TGTTAACCCT AAAACGTTTG ATACCAATGA TGGTGACGTT 
TTAGAAGGAA CACAGAAAAA TAGCAAAACA CATCACATCA TTAAAGGGTA CCAGAAAGAA 
TGTACAATAG TTCCCTCGAT AGAACAGTCA GTAATTTACT CTGATAGTTC GGGATGCATA 
GAATACACTA GGCCTAAGTC ATTGAGTGTC ACGTGTCCTG ACATGAAGAC AGCTGAAGCC 
GATGGGTGTC CTGTAGACAG TCCTGTCGAA TCTCTGGCTA GTCTGTTTGA GATTTCGTAT 
GACAAATCTC AAGTCAGCCC CACAGATGTT TGCTTTGAGC TCAGTGATGG CCTTCATATG 
AACTGTAAAC AAGTAGCACA TTGCATTATA GAATATACAG AGACCTCCAA ACAATTTCAC 
AGACCCGACA CCCTGTCTGA CAAACAAGCA AGACAAAACG AGTCAGTTGA AACAATTGAG 
CCGGATATTG ATATTTTGTC TAGTGCATGT ACTGATCCTT TCACAAATGC ACAATCCCCT 
TTTGATTATT CCCAGCTGAA CAGTAGAAAC AATGAATACA GCTCAGACAC AAATAATAAT 
ATTAAAGCCA TTACTTTTGA AGAGTGTGCC TCAGGTGAAC AGATCTCTTT GCCCAATTCT 
TTGCAAAAAT ACAGCAGGTT TAGTATCAAT GCTGGATCTT TACTCTCAAA AGGTGGTGAT 
TGCAAAGACA ATGCCCCTCC TAGTCAGGCT ACTCCCTTTA AAGCACCTGA AATGAGGACT 
GCAATTGACA GCAAAGATAA TGTTGCTAAA CCTTCTGAAT TCAATGAATT CAGCAGCCAT 
AGAGATGCTA TACTAAAGCA GCTCTGTGAT TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC 
TCGTCTGCAG TGGAAGGAGA GCAGACAGCA GGAGGATTTC AGTTCAAGGG CACAGACGGC 
ATCAGTCAGA GCAGAACTGC AGTGTTGCAA ACCTTGAGTC CCTCACACAC ACATGACCAA 
TGCAACACAC ATGGAGAGGT TAATGCAGAC TCTACAAACT GTGAATTTTC ACATATTGGC 
CATTTGCTTG CACACAAACA TCCGGAGCCC GTCCAAGAGA AAAGCACATT ACCACTGAGT 
GGCTCACTCA AGGTCGATGA TACTTATGCA TTGGAAAACA TGGAGAGAAA GCTTGAAACT 
TTCATGGCTA GCTCTCCTGA AAGCACTGAT TCAATATGTG AAACTTCTGA TCCTCTATGT 
AAGCAACCTT TGCATCTGCG CAACTCGGAG ACCGAGGGTG CATTTGTTTC ATGTTGCCGT 
ATTTTACCCA TCACTAATTT AGAGGCCATT TTGGAGTTGG ACCGTTCACC GGAGAGCTCA 
TTTGTGATAG AAAATGGACT GGGGGAGCAA CAGGGAGGAA GTCCATTATC AGAAGGTGGA 
TTCAGTGAGG AATGGAGCTC TTGTGATGAC GTCACAAACC GCCCACATGA GAACATTTCT 
GCCATGTGTA CAAAAGCCAA CGTAGCCACT GTCATGAATG AACAAGATGC TGGAGAAACA 
ATGAGCTCTT TATCAAATGG TTTTGATAGT GATGGTTATA GTGTTTCCAG TTTTGTGTCA 
CAAGACCTTG ACTCCAGTAC CAACAGTGAA GAGACGGAGG ACAGCACTCA GTCATCATCT 
AGTCTTCGTG GTTCAAGAAA TAAGAGAACC CAATCAGGGA AGGGTTCTGT GTTTTCTGTT 
TTTTCTCGAA TGCCCTCATT CCGCAAGATT AAACGAGAAC ACAAAGGCAA TAGCAAGGTT 
GATCCAGAAA TTAAAGATTC CAATGAGGAT GGAGATGAAC GGGGTGAGTT GAAGTACAAT 
AGGTCAACCA CACATAACAA ATCCAACCCT TCACTGTACA ATACCTCTTT TTCTCAAAGT 
ACAGATCATC TTACAGACAT TACAAAGTTT GTTGACCACT CCAATGATGA TATTTTTGAA 
AAAGCTTTTG CTTTAAACAT GCAGAATAGA GAGAAGTATA CACAATCCCA AAATTTGTAT 
CAGCAGCACA AAGATGGAGA TGCACTGAAT TTTAGAGTTT CCCCAACGAC AGAGGGATTG 
CAACAAAAAA GAAGCAAGAG TACTGATAAC TTAAACTTGC GCATGAAGCT CGCAATGGCT 
CACAAGTCTC TGTCTAGCCT GTTTGAGTCC AGATATCCTG AGAGGGACAA CCAGGAGCAG 
ATTGCACAGT CAGAGAATGA GGAGGTAAAA ACCAAGCAGT CCTGGAGGAA GTCTAAACGG 
TCTAAGGAAG CAGAACTTTG CAAAAGAACT CTATCGGTTC CTGGGATGGT CTGTGACAAG 
TCCATACATC AGAGCCATAT CGATTGTGCC TTCTGTGCTC CTCAGAACAG GTCCAGACTT 
AATAGCTCAC CAGCTTCCCA AAAGAGTTTG AGGAGCACGT GCCATTCTGA TTCACAGAGT 
TTGAAATCTG GTCTTCAGGA ACAGTCTGAA GACAGAAAAT ATCTGGAAGG AGGTGAGCTG 
CCTTGTGTGC CTTACTTAGA TTCTGACCAG ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA 
GAGGATAGGT CGCAATCACC TGTACACCAA ACTGTTTTTG CACTTGCAAA CCAGATGTCA 
CCTACCTGGA CCAGATCTTT AAGCTGTTTT GAGACCACTG ATACGCCAAC GAGGCCCATG 
AGCCCTAAAC CTCATAGTCC TGGTCTGGGC TGGACACACC GGAGGAGCTT CCGTTACCCT 
TCGAGATCTG TTGCATCTTC TCTCTGCTCA CTCGGTCAAG GAGTGAGCAC TGACGGTCTC 
TCCGATCCAC CTCAGAGACC GACGTCTCTG AAACCAAGGA CAGCACAACT TACAACAACC 
CACTCTTTTG ATTCTGAGTC CTTACTAGAG GACAGCAGTT CAGACAGCCA GTCTCAAAAT 
AGCCTGAATT CAGCATCCCT GATCAACAAA CCAGAGCATG TACAGCACAG TACTGAGCCA 
GCCATCCAGT CAGACGAGAG AAGACAACAG GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC 
AGACAGCAGG GTAAAGCTGC ACCCAGACCC ATCTCAGACC TCTACGGTTG GACCGTGTCT 
CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT CTGGAGAGGA AGAGCAATTC GACCGAAACC 
CGTCGGCGTT CATGCTCATA TGAAACGTTG ACTGATGTGG AGAACACCCA TAAAAAGAAG 
CTTAACACGC GGCAAAGTTT GCGTCAGCTT AGCTCACCAA CATCTCAGAA GCACGAGAAA 
GTGTGCGCTA GACTTTCCCT CACTTCTCCA GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC 
CACTTCTTCT CCCAGAGCAC ACCTGTTGGA CTGGACTTTT TGGGCTGGCC TCACCGTGCA 
TCATTTTCAG AATCGAATCT CAGCACACCA GCACAAGAGG CACATGAACA GGACCCTCCT 
CTGGCCTTGG TGATAACTGA TGGACCGCAG GATAAGTCTG GCCTTGCGGA TGAGGTGGGG 
AGTGAAGATG ACCTGTACAA TGAGTTTCGC AGCTCCTCCA ACCGATTCGG ACACCCAGGA 
GGAGGAGGAG AACAGCTGGC CATAAATGAG CTGATCAGTG ATGGCAGCGT GTGCGCGGAG 
GCTCTATGGG ACCACGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGAGACGTC 
ATCGAAGTAG TGGACGCCAC AAACAAGGAG TGGTGGTGGG GCCGGGTGCT GGATAGCGAG 
GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA CGGGTGAACC AAGATGAGCC CATGGAGGAA 
TACTTAGCCC ATCTGGACGG GGCCTCGGAG GGGGGTGGGG CCAGCATGGG GGACCCCTTA 
GGGCCCGGTC TGCCCTGCAA GGAGCAGATG AGAGCCAACG TCATCAATGA GATCATGAGC 
ACAGAGAGGG ATTACATCAA ACACCTGAAG GACATCTGTG AGGGCTATAT AAAACAGTGT 
CGGAAGAGGA CAGATATGTT TACTGAGGAG CAACTGCGCA CTATCTTTGG GAACATCGAT 
GAGCTCTACC GTTTCCAGAA GAAGTTTCTC AAAGCCTTGG AGAAGAAATT CAACAAAGAC 
CATCCTCACC TCAGCGAGAT TGGCTCCTGC TTCTTAGAGC ATCAAACAAA CTTTCAGATC 
TACTCTGAAT ATTGCAACAA CCACCCCAAT GCCTGCGTGC AGCTCTCCAA ACTGATGAAG 
ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC TGTCGCCTGC TCCAGAAGAT GATCGACATT 
TCCTTGGATG GATTCCTGCT TACTCCTGTT CAGAAGATCT GCAAGTATCC TCTGCAGCTG 
GCTGAACTGC TGAAGTACAC CAACCCACAG CACAGAGATT ATAAGGATGT AGAAGCTGCC 
TTAAATGCAA TGAAGAATGT GGCCCGACTG ATCAATGAGA GAAAGAGGCG TCTGGAGAAT 
ATTGACAAAA TTGCTCAGTG GCAAAGCTCC ATAGAAGACT GGGAGGGTGA AGACATCTTG 
AGCAGAAGCT CTGATCTGAT TTTCTCAGGA GACCTGACCA AGATCTCCCA GCCACAGGGC 
AAAGGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAAC TGGTTTTCTG TAAGAAGGAT 
CTGCTTCGGA GGGACATCCT GTACTACAAG GGTCGGTTAG ACATGGATCA GATGCAAGTG 
GTGGATGTGG AGGATGGGAA GGATAAGGAC TTTAACGTGA GTGTGAAGAA TGCCTTGAAA 
TTATGTTCTC CTGTGGGAGA GGAGGTCCAC CTTCTGTGTG CCAAGAAACC CGAGCAGAAG 
CAGCGCTGGC TGCGAGCCTT TGCGGATGAA CGGGAACAGG TCCAGCACGA CCTCGAAACA 
GGTTTTACGA TCACAGAGGT CCAGAAGAAG CAGGCCATGT TGAATGCATC TAAAAGTCAT 
CCAACAGGAA AGCCCAAAGC TGTGACCAGG CCCTACTATG ACTTCCTGCT GCGTCAGAAG 
CACCCCACGC TTCCCACCTC CCTGCCCCAG CAGCAGGTCA TCATGTTGGC CGAACCCAAA 
CGCAAGACCT CCAATTTCTG GCACAACATC GGACGCTTGA CACCGTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515439.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370925.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X3, mRNA.

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CloneID	OSi145733
Clone ID Related Accession (Same CDS sequence)	XM_016515439.1
Accession Version	XM_016515439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGTG CTATATACTT CATACGAAGC TTCTTTAAGG CCCCAGCTTT AGAGGACTCT 
GGGAGTGAGG CAGCCTGTCT GGATGATGGG GAGTCTCTGC AGGAGGGGCA TGGAGAGCAA 
CAGGACTATC AGGAATGGTG TGAGCCAGTT TCTGAAGCAG CACAGGGCAC AAAAGAGGAA 
TATTTTGACA CACACTCATG GCACAGTGAC ACTTTCTCTG GCTTGAGTCA TGAAACTAAG 
GACTGCTGCA GGACACCGCT CAGCTCTGTG GGTCATCTGG ACTCACACTC ACCTTCGGTT 
AGAAGGAGAG ATCCCTCTTC ATCAAAACTG CATCATTCTG TCAATCATGC TGACAGTCTC 
AGTAGAACAG AGGATAGAGA ACAATTGCAA AAGCAGACTG AAAATATGGG GATGATGTGC 
ATGGGAGTTG ATGGCACTGA AATGTCTCAA GCTAAGGGTG CTCCGGAATG TGAGGAGGTT 
TTCAGCAATT TTCCAGCGGG ACCTGATGTT GATTCTTCTT CAAGGAATGA CCAATCCTAC 
ATCGTAACAC CCCTACTGGC ATTAGGGAAC GCTGTAGAAC CACAAACACT CACTGAGCCA 
TGTGCTAATG ACTCATTATT GACCTTAACA GACTGTGATG GAGCCAAGTC TCAGTTTGCT 
GACACCTTTG AGAGAAGATC AAAATGGGAT CAAATTAACA AAAATATCAC AGACACTCAG 
TCAGGACACA ATGAGCCGCG AATACAGTTC ATAACCTCAA GTGAGAAGCA GTTACAGCAA 
ACTTATATTG ACAGTAGTAA CACTGAAGTA CAGTTGATTT CGAATGCATC TGTTGCGAAT 
AGTAAAGATA ACGAATGTCG TGAGCTGGGT GTTGATGAAA ATAAGATCAG TTTATCTGTA 
TCACGTCAAC TTTCTGTATA TGAGTCTGTA TCAGTTGAGA AGCCTATTGA ACAGGAATTT 
GAGGAGTCAA CAAAACACAG CCAATCACAG CCCTCAGTAG AGCACAGCAA ATCACAGCCC 
TCAGTAGAGC ACAGCAAATC ACAGCCCTCA GTAGAGCACA GCCAATCACA GGCATCAACA 
GAACAAATCC AATCACAGGA TTCTGTAGAA TGCAGCCAAT CACGGAATTC AGCAGAACAC 
AGGCAATCAC GAGACTCAAT AGAACACAGT CAGTCACAGG ATTCAGTAGA ATGTAGGCAA 
TCACAGAATT CAGTAGAAGA CAGCCAACTA CAAGACTCAG TAGAACATAG TCAGCCAAAG 
GATTTTGTAG AATGCAGCCA ATCACATGAT TCTATAGAAT GCAGCCACTC ACTGTATTCA 
GTAAAACACA GGCAATCACA AGACTCAATA GAACACGGCC AGTCACAGAG CTCAGTAGAA 
TACACCAAAT CACAGGATTC TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACAGA CCCCAGTAGA ACACACCAAA TCACAGGATT CTGTAAAATG CAGTCAATCA 
CAGAATTCAG CAGAACACAA CCAATCACAG GACTCAGTAG AACACAGTCA GTCACAGAGC 
TCAGAAGACA TGCCATCCCA AAATTTCATC AAACACAGCC AATCACAGAC CCCAGTAGAA 
CACCCCAAAT CACAGGATTG TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACGGG ACTCAGTAGA ACACAGCCAG TCACACAGCT CAGTAGAAGA CATGCTATCG 
CAAAATGTAA TTAACCACAA CAAATCACAA GACCCAGTAG AATATACTCT GTCACTGACC 
TCACTACAAC ACACCCAATC ACAGGATTCT ATAGAACTTA GCCCATCAAA TGATTCTGTA 
GAATGCAGCC AATCACAGGA TTCAGTAGAA AACACATTAG AAAGTAGAAA ACGTCAGTCA 
CATACTACAT CAGAACACAT TCAGTTACAG AAGTCTATAG AATGTAGCCA ATCACAGAAT 
TCCATACAAC ACAGGAAATC ACAGGATTTA GCAGAACAAA ACCAATCACA GAACACCATA 
GAGCACAGCC AATCACAAAC TCCAGTGGAA CACAGCCAAT CACAAACTTC AGTGCAACAC 
AGCCAATCAC AAACTTCAGT GGAACACAGC CAATCACAAG TTGATTCCAT TAAGGCAAAT 
TCTATGAATA CCCAGTGGAC TGTGACCGAG TCAGACTCTG GTTTTATTTC AAACACAGCT 
TCTCTTTATG AAAAGGGACA ATTAAAACAG GACTCCGTGG ACGTATGCAT GAATTGTGAA 
GTCAGTCAGT CCTTGAAAAC ATCTGGAAGG CTTTCATCAA AGGATACTGA CAAAGAACTC 
GATTTGAACT TCGAAAAGAA TAACAGTCTA AATTTAAAAA AGCAGAACTT AAATGACACC 
CCAAGTGTTG TTTCTGAATG CGAGTCTTTT GCAGCTGAGA GAGCTTTCTC AAATGTGCCT 
AATGCAACTT CTGCTGGAGT GTCAGATTCA CTGAATCAGC ATGTGATCAA TGACCGGACC 
TCAGAACAGT CAGATCTGCA GGCCAGACCT CATTACGGTC AACAAAATTT CCCAGTTTTA 
CAAATGCACA CTGAAAAAAA GGATTACTCA GTTAGTAGTT ATCACATGGA CAGTCATTGC 
TTAAAGGACA TTGTTTTAGG GGAACATGAG CCTGAGGCAG ATTGTTATGA AACCCGTCAG 
TGCCGTAATA GCAGAATTAA CTGTAACACA CAGGGTGAAT TCACTGAAGC ACAAGTGTAT 
GTTAACCCTA AAACGTTTGA TACCAATGAT GGTGACGTTT TAGAAGGAAC ACAGAAAAAT 
AGCAAAACAC ATCACATCAT TAAAGGGTAC CAGAAAGAAT GTACAATAGT TCCCTCGATA 
GAACAGTCAG TAATTTACTC TGATAGTTCG GGATGCATAG AATACACTAG GCCTAAGTCA 
TTGAGTGTCA CGTGTCCTGA CATGAAGACA GCTGAAGCCG ATGGGTGTCC TGTAGACAGT 
CCTGTCGAAT CTCTGGCTAG TCTGTTTGAG ATTTCGTATG ACAAATCTCA AGTCAGCCCC 
ACAGATGTTT GCTTTGAGCT CAGTGATGGC CTTCATATGA ACTGTAAACA AGTAGCACAT 
TGCATTATAG AATATACAGA GACCTCCAAA CAATTTCACA GACCCGACAC CCTGTCTGAC 
AAACAAGCAA GACAAAACGA GTCAGTTGAA ACAATTGAGC CGGATATTGA TATTTTGTCT 
AGTGCATGTA CTGATCCTTT CACAAATGCA CAATCCCCTT TTGATTATTC CCAGCTGAAC 
AGTAGAAACA ATGAATACAG CTCAGACACA AATAATAATA TTAAAGCCAT TACTTTTGAA 
GAGTGTGCCT CAGGTGAACA GATCTCTTTG CCCAATTCTT TGCAAAAATA CAGCAGGTTT 
AGTATCAATG CTGGATCTTT ACTCTCAAAA GGTGGTGATT GCAAAGACAA TGCCCCTCCT 
AGTCAGGCTA CTCCCTTTAA AGCACCTGAA ATGAGGACTG CAATTGACAG CAAAGATAAT 
GTTGCTAAAC CTTCTGAATT CAATGAATTC AGCAGCCATA GAGATGCTAT ACTAAAGCAG 
CTCTGTGATT GCTGTGGTGG AAAGGAGTGC CAGCTGCCCT CGTCTGCAGT GGAAGGAGAG 
CAGACAGCAG GAGGATTTCA GTTCAAGGGC ACAGACGGCA TCAGTCAGAG CAGAACTGCA 
GTGTTGCAAA CCTTGAGTCC CTCACACACA CATGACCAAT GCAACACACA TGGAGAGGTT 
AATGCAGACT CTACAAACTG TGAATTTTCA CATATTGGCC ATTTGCTTGC ACACAAACAT 
CCGGAGCCCG TCCAAGAGAA AAGCACATTA CCACTGAGTG GCTCACTCAA GGTCGATGAT 
ACTTATGCAT TGGAAAACAT GGAGAGAAAG CTTGAAACTT TCATGGCTAG CTCTCCTGAA 
AGCACTGATT CAATATGTGA AACTTCTGAT CCTCTATGTA AGCAACCTTT GCATCTGCGC 
AACTCGGAGA CCGAGGGTGC ATTTGTTTCA TGTTGCCGTA TTTTACCCAT CACTAATTTA 
GAGGCCATTT TGGAGTTGGA CCGTTCACCG GAGAGCTCAT TTGTGATAGA AAATGGACTG 
GGGGAGCAAC AGGGAGGAAG TCCATTATCA GAAGGTGGAT TCAGTGAGGA ATGGAGCTCT 
TGTGATGACG TCACAAACCG CCCACATGAG AACATTTCTG CCATGTGTAC AAAAGCCAAC 
GTAGCCACTG TCATGAATGA ACAAGATGCT GGAGAAACAA TGAGCTCTTT ATCAAATGGT 
TTTGATAGTG ATGGTTATAG TGTTTCCAGT TTTGTGTCAC AAGACCTTGA CTCCAGTACC 
AACAGTGAAG AGACGGAGGA CAGCACTCAG TCATCATCTA GTCTTCGTGG TTCAAGAAAT 
AAGAGAACCC AATCAGGGAA GGGTTCTGTG TTTTCTGTTT TTTCTCGAAT GCCCTCATTC 
CGCAAGATTA AACGAGAACA CAAAGGCAAT AGCAAGGTTG ATCCAGAAAT TAAAGATTCC 
AATGAGGATG GAGATGAACG GGGTGAGTTG AAGTACAATA GGTCAACCAC ACATAACAAA 
TCCAACCCTT CACTGTACAA TACCTCTTTT TCTCAAAGTA CAGATCATCT TACAGACATT 
ACAAAGTTTG TTGACCACTC CAATGATGAT ATTTTTGAAA AAGCTTTTGC TTTAAACATG 
CAGAATAGAG AGAAGTATAC ACAATCCCAA AATTTGTATC AGCAGCACAA AGATGGAGAT 
GCACTGAATT TTAGAGTTTC CCCAACGACA GAGGGATTGC AACAAAAAAG AAGCAAGAGT 
ACTGATAACT TAAACTTGCG CATGAAGCTC GCAATGGCTC ACAAGTCTCT GTCTAGCCTG 
TTTGAGTCCA GATATCCTGA GAGGGACAAC CAGGAGCAGA TTGCACAGTC AGAGAATGAG 
GAGGTAAAAA CCAAGCAGTC CTGGAGGAAG TCTAAACGGT CTAAGGAAGC AGAACTTTGC 
AAAAGAACTC TATCGGTTCC TGGGATGGTC TGTGACAAGT CCATACATCA GAGCCATATC 
GATTGTGCCT TCTGTGCTCC TCAGAACAGG TCCAGACTTA ATAGCTCACC AGCTTCCCAA 
AAGAGTTTGA GGAGCACGTG CCATTCTGAT TCACAGAGTT TGAAATCTGG TCTTCAGGAA 
CAGTCTGAAG ACAGAAAATA TCTGGAAGGA GGTGAGCTGC CTTGTGTGCC TTACTTAGAT 
TCTGACCAGA TCTCAGAAGA TGGCTTAGAG TCCAGTGTAG AGGATAGGTC GCAATCACCT 
GTACACCAAA CTGTTTTTGC ACTTGCAAAC CAGATGTCAC CTACCTGGAC CAGATCTTTA 
AGCTGTTTTG AGACCACTGA TACGCCAACG AGGCCCATGA GCCCTAAACC TCATAGTCCT 
GGTCTGGGCT GGACACACCG GAGGAGCTTC CGTTACCCTT CGAGATCTGT TGCATCTTCT 
CTCTGCTCAC TCGGTCAAGG AGTGAGCACT GACGGTCTCT CCGATCCACC TCAGAGACCG 
ACGTCTCTGA AACCAAGGAC AGCACAACTT ACAACAACCC ACTCTTTTGA TTCTGAGTCC 
TTACTAGAGG ACAGCAGTTC AGACAGCCAG TCTCAAAATA GCCTGAATTC AGCATCCCTG 
ATCAACAAAC CAGAGCATGT ACAGCACAGT ACTGAGCCAG CCATCCAGTC AGACGAGAGA 
AGACAACAGG GAAGTGCATG TGTGCTTCGG GTGAAGTCCA GACAGCAGGG TAAAGCTGCA 
CCCAGACCCA TCTCAGACCT CTACGGTTGG ACCGTGTCTC TTCAGGGCAT CAGGGAGGTG 
GCGCTTGATC TGGAGAGGAA GAGCAATTCG ACCGAAACCC GTCGGCGTTC ATGCTCATAT 
GAAACGTTGA CTGATGTGGA GAACACCCAT AAAAAGAAGC TTAACACGCG GCAAAGTTTG 
CGTCAGCTTA GCTCACCAAC ATCTCAGAAG CACGAGAAAG TGTGCGCTAG ACTTTCCCTC 
ACTTCTCCAG AGCAGTTCCC CTCTGTTCCT CTCAAAGACC ACTTCTTCTC CCAGAGCACA 
CCTGTTGGAC TGGACTTTTT GGGCTGGCCT CACCGTGCAT CATTTTCAGA ATCGAATCTC 
AGCACACCAG CACAAGAGGC ACATGAACAG GACCCTCCTC TGGCCTTGGT GATAACTGAT 
GGACCGCAGG ATAAGTCTGG CCTTGCGGAT GAGGTGGGGA GTGAAGATGA CCTGTACAAT 
GAGTTTCGCA GCTCCTCCAA CCGATTCGGA CACCCAGGAG GAGGAGGAGA ACAGCTGGCC 
ATAAATGAGC TGATCAGTGA TGGCAGCGTG TGCGCGGAGG CTCTATGGGA CCACGTCACC 
ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGAGACGTCA TCGAAGTAGT GGACGCCACA 
AACAAGGAGT GGTGGTGGGG CCGGGTGCTG GATAGCGAGG GCTGGTTCCC CGCCAGCTTT 
GTTCGGTTAC GGGTGAACCA AGATGAGCCC ATGGAGGAAT ACTTAGCCCA TCTGGACGGG 
GCCTCGGAGG GGGGTGGGGC CAGCATGGGG GACCCCTTAG GGCCCGGTCT GCCCTGCAAG 
GAGCAGATGA GAGCCAACGT CATCAATGAG ATCATGAGCA CAGAGAGGGA TTACATCAAA 
CACCTGAAGG ACATCTGTGA GGGCTATATA AAACAGTGTC GGAAGAGGAC AGATATGTTT 
ACTGAGGAGC AACTGCGCAC TATCTTTGGG AACATCGATG AGCTCTACCG TTTCCAGAAG 
AAGTTTCTCA AAGCCTTGGA GAAGAAATTC AACAAAGACC ATCCTCACCT CAGCGAGATT 
GGCTCCTGCT TCTTAGAGCA TCAAACAAAC TTTCAGATCT ACTCTGAATA TTGCAACAAC 
CACCCCAATG CCTGCGTGCA GCTCTCCAAA CTGATGAAGA TCAAGAAGTA TGTGTTCTTC 
TTTGAGGCCT GTCGCCTGCT CCAGAAGATG ATCGACATTT CCTTGGATGG ATTCCTGCTT 
ACTCCTGTTC AGAAGATCTG CAAGTATCCT CTGCAGCTGG CTGAACTGCT GAAGTACACC 
AACCCACAGC ACAGAGATTA TAAGGATGTA GAAGCTGCCT TAAATGCAAT GAAGAATGTG 
GCCCGACTGA TCAATGAGAG AAAGAGGCGT CTGGAGAATA TTGACAAAAT TGCTCAGTGG 
CAAAGCTCCA TAGAAGACTG GGAGGGTGAA GACATCTTGA GCAGAAGCTC TGATCTGATT 
TTCTCAGGAG ACCTGACCAA GATCTCCCAG CCACAGGGCA AAGGCCAGCA GCGAATGTTC 
TTTCTCTTTG ACCACCAACT GGTTTTCTGT AAGAAGGATC TGCTTCGGAG GGACATCCTG 
TACTACAAGG GTCGGTTAGA CATGGATCAG ATGCAAGTGG TGGATGTGGA GGATGGGAAG 
GATAAGGACT TTAACGTGAG TGTGAAGAAT GCCTTGAAAT TATGTTCTCC TGTGGGAGAG 
GAGGTCCACC TTCTGTGTGC CAAGAAACCC GAGCAGAAGC AGCGCTGGCT GCGAGCCTTT 
GCGGATGAAC GGGAACAGGT CCAGCACGAC CTCGAAACAG GTTTTACGAT CACAGAGGTC 
CAGAAGAAGC AGGCCATGTT GAATGCATCT AAAAGTCATC CAACAGGAAA GCCCAAAGCT 
GTGACCAGGC CCTACTATGA CTTCCTGCTG CGTCAGAAGC ACCCCACGCT TCCCACCTCC 
CTGCCCCAGC AGCAGGTCAT CATGTTGGCC GAACCCAAAC GCAAGACCTC CAATTTCTGG 
CACAACATCG GACGCTTGAC ACCGTTCAAA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370925.1
CDS	164..8059
Translation	MLSAIYFIRSFFKAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515439.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515439.1

ORF Insert Sequence:

ATGTTGAGTG CTATATACTT CATACGAAGC TTCTTTAAGG CCCCAGCTTT AGAGGACTCT 
GGGAGTGAGG CAGCCTGTCT GGATGATGGG GAGTCTCTGC AGGAGGGGCA TGGAGAGCAA 
CAGGACTATC AGGAATGGTG TGAGCCAGTT TCTGAAGCAG CACAGGGCAC AAAAGAGGAA 
TATTTTGACA CACACTCATG GCACAGTGAC ACTTTCTCTG GCTTGAGTCA TGAAACTAAG 
GACTGCTGCA GGACACCGCT CAGCTCTGTG GGTCATCTGG ACTCACACTC ACCTTCGGTT 
AGAAGGAGAG ATCCCTCTTC ATCAAAACTG CATCATTCTG TCAATCATGC TGACAGTCTC 
AGTAGAACAG AGGATAGAGA ACAATTGCAA AAGCAGACTG AAAATATGGG GATGATGTGC 
ATGGGAGTTG ATGGCACTGA AATGTCTCAA GCTAAGGGTG CTCCGGAATG TGAGGAGGTT 
TTCAGCAATT TTCCAGCGGG ACCTGATGTT GATTCTTCTT CAAGGAATGA CCAATCCTAC 
ATCGTAACAC CCCTACTGGC ATTAGGGAAC GCTGTAGAAC CACAAACACT CACTGAGCCA 
TGTGCTAATG ACTCATTATT GACCTTAACA GACTGTGATG GAGCCAAGTC TCAGTTTGCT 
GACACCTTTG AGAGAAGATC AAAATGGGAT CAAATTAACA AAAATATCAC AGACACTCAG 
TCAGGACACA ATGAGCCGCG AATACAGTTC ATAACCTCAA GTGAGAAGCA GTTACAGCAA 
ACTTATATTG ACAGTAGTAA CACTGAAGTA CAGTTGATTT CGAATGCATC TGTTGCGAAT 
AGTAAAGATA ACGAATGTCG TGAGCTGGGT GTTGATGAAA ATAAGATCAG TTTATCTGTA 
TCACGTCAAC TTTCTGTATA TGAGTCTGTA TCAGTTGAGA AGCCTATTGA ACAGGAATTT 
GAGGAGTCAA CAAAACACAG CCAATCACAG CCCTCAGTAG AGCACAGCAA ATCACAGCCC 
TCAGTAGAGC ACAGCAAATC ACAGCCCTCA GTAGAGCACA GCCAATCACA GGCATCAACA 
GAACAAATCC AATCACAGGA TTCTGTAGAA TGCAGCCAAT CACGGAATTC AGCAGAACAC 
AGGCAATCAC GAGACTCAAT AGAACACAGT CAGTCACAGG ATTCAGTAGA ATGTAGGCAA 
TCACAGAATT CAGTAGAAGA CAGCCAACTA CAAGACTCAG TAGAACATAG TCAGCCAAAG 
GATTTTGTAG AATGCAGCCA ATCACATGAT TCTATAGAAT GCAGCCACTC ACTGTATTCA 
GTAAAACACA GGCAATCACA AGACTCAATA GAACACGGCC AGTCACAGAG CTCAGTAGAA 
TACACCAAAT CACAGGATTC TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACAGA CCCCAGTAGA ACACACCAAA TCACAGGATT CTGTAAAATG CAGTCAATCA 
CAGAATTCAG CAGAACACAA CCAATCACAG GACTCAGTAG AACACAGTCA GTCACAGAGC 
TCAGAAGACA TGCCATCCCA AAATTTCATC AAACACAGCC AATCACAGAC CCCAGTAGAA 
CACCCCAAAT CACAGGATTG TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACGGG ACTCAGTAGA ACACAGCCAG TCACACAGCT CAGTAGAAGA CATGCTATCG 
CAAAATGTAA TTAACCACAA CAAATCACAA GACCCAGTAG AATATACTCT GTCACTGACC 
TCACTACAAC ACACCCAATC ACAGGATTCT ATAGAACTTA GCCCATCAAA TGATTCTGTA 
GAATGCAGCC AATCACAGGA TTCAGTAGAA AACACATTAG AAAGTAGAAA ACGTCAGTCA 
CATACTACAT CAGAACACAT TCAGTTACAG AAGTCTATAG AATGTAGCCA ATCACAGAAT 
TCCATACAAC ACAGGAAATC ACAGGATTTA GCAGAACAAA ACCAATCACA GAACACCATA 
GAGCACAGCC AATCACAAAC TCCAGTGGAA CACAGCCAAT CACAAACTTC AGTGCAACAC 
AGCCAATCAC AAACTTCAGT GGAACACAGC CAATCACAAG TTGATTCCAT TAAGGCAAAT 
TCTATGAATA CCCAGTGGAC TGTGACCGAG TCAGACTCTG GTTTTATTTC AAACACAGCT 
TCTCTTTATG AAAAGGGACA ATTAAAACAG GACTCCGTGG ACGTATGCAT GAATTGTGAA 
GTCAGTCAGT CCTTGAAAAC ATCTGGAAGG CTTTCATCAA AGGATACTGA CAAAGAACTC 
GATTTGAACT TCGAAAAGAA TAACAGTCTA AATTTAAAAA AGCAGAACTT AAATGACACC 
CCAAGTGTTG TTTCTGAATG CGAGTCTTTT GCAGCTGAGA GAGCTTTCTC AAATGTGCCT 
AATGCAACTT CTGCTGGAGT GTCAGATTCA CTGAATCAGC ATGTGATCAA TGACCGGACC 
TCAGAACAGT CAGATCTGCA GGCCAGACCT CATTACGGTC AACAAAATTT CCCAGTTTTA 
CAAATGCACA CTGAAAAAAA GGATTACTCA GTTAGTAGTT ATCACATGGA CAGTCATTGC 
TTAAAGGACA TTGTTTTAGG GGAACATGAG CCTGAGGCAG ATTGTTATGA AACCCGTCAG 
TGCCGTAATA GCAGAATTAA CTGTAACACA CAGGGTGAAT TCACTGAAGC ACAAGTGTAT 
GTTAACCCTA AAACGTTTGA TACCAATGAT GGTGACGTTT TAGAAGGAAC ACAGAAAAAT 
AGCAAAACAC ATCACATCAT TAAAGGGTAC CAGAAAGAAT GTACAATAGT TCCCTCGATA 
GAACAGTCAG TAATTTACTC TGATAGTTCG GGATGCATAG AATACACTAG GCCTAAGTCA 
TTGAGTGTCA CGTGTCCTGA CATGAAGACA GCTGAAGCCG ATGGGTGTCC TGTAGACAGT 
CCTGTCGAAT CTCTGGCTAG TCTGTTTGAG ATTTCGTATG ACAAATCTCA AGTCAGCCCC 
ACAGATGTTT GCTTTGAGCT CAGTGATGGC CTTCATATGA ACTGTAAACA AGTAGCACAT 
TGCATTATAG AATATACAGA GACCTCCAAA CAATTTCACA GACCCGACAC CCTGTCTGAC 
AAACAAGCAA GACAAAACGA GTCAGTTGAA ACAATTGAGC CGGATATTGA TATTTTGTCT 
AGTGCATGTA CTGATCCTTT CACAAATGCA CAATCCCCTT TTGATTATTC CCAGCTGAAC 
AGTAGAAACA ATGAATACAG CTCAGACACA AATAATAATA TTAAAGCCAT TACTTTTGAA 
GAGTGTGCCT CAGGTGAACA GATCTCTTTG CCCAATTCTT TGCAAAAATA CAGCAGGTTT 
AGTATCAATG CTGGATCTTT ACTCTCAAAA GGTGGTGATT GCAAAGACAA TGCCCCTCCT 
AGTCAGGCTA CTCCCTTTAA AGCACCTGAA ATGAGGACTG CAATTGACAG CAAAGATAAT 
GTTGCTAAAC CTTCTGAATT CAATGAATTC AGCAGCCATA GAGATGCTAT ACTAAAGCAG 
CTCTGTGATT GCTGTGGTGG AAAGGAGTGC CAGCTGCCCT CGTCTGCAGT GGAAGGAGAG 
CAGACAGCAG GAGGATTTCA GTTCAAGGGC ACAGACGGCA TCAGTCAGAG CAGAACTGCA 
GTGTTGCAAA CCTTGAGTCC CTCACACACA CATGACCAAT GCAACACACA TGGAGAGGTT 
AATGCAGACT CTACAAACTG TGAATTTTCA CATATTGGCC ATTTGCTTGC ACACAAACAT 
CCGGAGCCCG TCCAAGAGAA AAGCACATTA CCACTGAGTG GCTCACTCAA GGTCGATGAT 
ACTTATGCAT TGGAAAACAT GGAGAGAAAG CTTGAAACTT TCATGGCTAG CTCTCCTGAA 
AGCACTGATT CAATATGTGA AACTTCTGAT CCTCTATGTA AGCAACCTTT GCATCTGCGC 
AACTCGGAGA CCGAGGGTGC ATTTGTTTCA TGTTGCCGTA TTTTACCCAT CACTAATTTA 
GAGGCCATTT TGGAGTTGGA CCGTTCACCG GAGAGCTCAT TTGTGATAGA AAATGGACTG 
GGGGAGCAAC AGGGAGGAAG TCCATTATCA GAAGGTGGAT TCAGTGAGGA ATGGAGCTCT 
TGTGATGACG TCACAAACCG CCCACATGAG AACATTTCTG CCATGTGTAC AAAAGCCAAC 
GTAGCCACTG TCATGAATGA ACAAGATGCT GGAGAAACAA TGAGCTCTTT ATCAAATGGT 
TTTGATAGTG ATGGTTATAG TGTTTCCAGT TTTGTGTCAC AAGACCTTGA CTCCAGTACC 
AACAGTGAAG AGACGGAGGA CAGCACTCAG TCATCATCTA GTCTTCGTGG TTCAAGAAAT 
AAGAGAACCC AATCAGGGAA GGGTTCTGTG TTTTCTGTTT TTTCTCGAAT GCCCTCATTC 
CGCAAGATTA AACGAGAACA CAAAGGCAAT AGCAAGGTTG ATCCAGAAAT TAAAGATTCC 
AATGAGGATG GAGATGAACG GGGTGAGTTG AAGTACAATA GGTCAACCAC ACATAACAAA 
TCCAACCCTT CACTGTACAA TACCTCTTTT TCTCAAAGTA CAGATCATCT TACAGACATT 
ACAAAGTTTG TTGACCACTC CAATGATGAT ATTTTTGAAA AAGCTTTTGC TTTAAACATG 
CAGAATAGAG AGAAGTATAC ACAATCCCAA AATTTGTATC AGCAGCACAA AGATGGAGAT 
GCACTGAATT TTAGAGTTTC CCCAACGACA GAGGGATTGC AACAAAAAAG AAGCAAGAGT 
ACTGATAACT TAAACTTGCG CATGAAGCTC GCAATGGCTC ACAAGTCTCT GTCTAGCCTG 
TTTGAGTCCA GATATCCTGA GAGGGACAAC CAGGAGCAGA TTGCACAGTC AGAGAATGAG 
GAGGTAAAAA CCAAGCAGTC CTGGAGGAAG TCTAAACGGT CTAAGGAAGC AGAACTTTGC 
AAAAGAACTC TATCGGTTCC TGGGATGGTC TGTGACAAGT CCATACATCA GAGCCATATC 
GATTGTGCCT TCTGTGCTCC TCAGAACAGG TCCAGACTTA ATAGCTCACC AGCTTCCCAA 
AAGAGTTTGA GGAGCACGTG CCATTCTGAT TCACAGAGTT TGAAATCTGG TCTTCAGGAA 
CAGTCTGAAG ACAGAAAATA TCTGGAAGGA GGTGAGCTGC CTTGTGTGCC TTACTTAGAT 
TCTGACCAGA TCTCAGAAGA TGGCTTAGAG TCCAGTGTAG AGGATAGGTC GCAATCACCT 
GTACACCAAA CTGTTTTTGC ACTTGCAAAC CAGATGTCAC CTACCTGGAC CAGATCTTTA 
AGCTGTTTTG AGACCACTGA TACGCCAACG AGGCCCATGA GCCCTAAACC TCATAGTCCT 
GGTCTGGGCT GGACACACCG GAGGAGCTTC CGTTACCCTT CGAGATCTGT TGCATCTTCT 
CTCTGCTCAC TCGGTCAAGG AGTGAGCACT GACGGTCTCT CCGATCCACC TCAGAGACCG 
ACGTCTCTGA AACCAAGGAC AGCACAACTT ACAACAACCC ACTCTTTTGA TTCTGAGTCC 
TTACTAGAGG ACAGCAGTTC AGACAGCCAG TCTCAAAATA GCCTGAATTC AGCATCCCTG 
ATCAACAAAC CAGAGCATGT ACAGCACAGT ACTGAGCCAG CCATCCAGTC AGACGAGAGA 
AGACAACAGG GAAGTGCATG TGTGCTTCGG GTGAAGTCCA GACAGCAGGG TAAAGCTGCA 
CCCAGACCCA TCTCAGACCT CTACGGTTGG ACCGTGTCTC TTCAGGGCAT CAGGGAGGTG 
GCGCTTGATC TGGAGAGGAA GAGCAATTCG ACCGAAACCC GTCGGCGTTC ATGCTCATAT 
GAAACGTTGA CTGATGTGGA GAACACCCAT AAAAAGAAGC TTAACACGCG GCAAAGTTTG 
CGTCAGCTTA GCTCACCAAC ATCTCAGAAG CACGAGAAAG TGTGCGCTAG ACTTTCCCTC 
ACTTCTCCAG AGCAGTTCCC CTCTGTTCCT CTCAAAGACC ACTTCTTCTC CCAGAGCACA 
CCTGTTGGAC TGGACTTTTT GGGCTGGCCT CACCGTGCAT CATTTTCAGA ATCGAATCTC 
AGCACACCAG CACAAGAGGC ACATGAACAG GACCCTCCTC TGGCCTTGGT GATAACTGAT 
GGACCGCAGG ATAAGTCTGG CCTTGCGGAT GAGGTGGGGA GTGAAGATGA CCTGTACAAT 
GAGTTTCGCA GCTCCTCCAA CCGATTCGGA CACCCAGGAG GAGGAGGAGA ACAGCTGGCC 
ATAAATGAGC TGATCAGTGA TGGCAGCGTG TGCGCGGAGG CTCTATGGGA CCACGTCACC 
ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGAGACGTCA TCGAAGTAGT GGACGCCACA 
AACAAGGAGT GGTGGTGGGG CCGGGTGCTG GATAGCGAGG GCTGGTTCCC CGCCAGCTTT 
GTTCGGTTAC GGGTGAACCA AGATGAGCCC ATGGAGGAAT ACTTAGCCCA TCTGGACGGG 
GCCTCGGAGG GGGGTGGGGC CAGCATGGGG GACCCCTTAG GGCCCGGTCT GCCCTGCAAG 
GAGCAGATGA GAGCCAACGT CATCAATGAG ATCATGAGCA CAGAGAGGGA TTACATCAAA 
CACCTGAAGG ACATCTGTGA GGGCTATATA AAACAGTGTC GGAAGAGGAC AGATATGTTT 
ACTGAGGAGC AACTGCGCAC TATCTTTGGG AACATCGATG AGCTCTACCG TTTCCAGAAG 
AAGTTTCTCA AAGCCTTGGA GAAGAAATTC AACAAAGACC ATCCTCACCT CAGCGAGATT 
GGCTCCTGCT TCTTAGAGCA TCAAACAAAC TTTCAGATCT ACTCTGAATA TTGCAACAAC 
CACCCCAATG CCTGCGTGCA GCTCTCCAAA CTGATGAAGA TCAAGAAGTA TGTGTTCTTC 
TTTGAGGCCT GTCGCCTGCT CCAGAAGATG ATCGACATTT CCTTGGATGG ATTCCTGCTT 
ACTCCTGTTC AGAAGATCTG CAAGTATCCT CTGCAGCTGG CTGAACTGCT GAAGTACACC 
AACCCACAGC ACAGAGATTA TAAGGATGTA GAAGCTGCCT TAAATGCAAT GAAGAATGTG 
GCCCGACTGA TCAATGAGAG AAAGAGGCGT CTGGAGAATA TTGACAAAAT TGCTCAGTGG 
CAAAGCTCCA TAGAAGACTG GGAGGGTGAA GACATCTTGA GCAGAAGCTC TGATCTGATT 
TTCTCAGGAG ACCTGACCAA GATCTCCCAG CCACAGGGCA AAGGCCAGCA GCGAATGTTC 
TTTCTCTTTG ACCACCAACT GGTTTTCTGT AAGAAGGATC TGCTTCGGAG GGACATCCTG 
TACTACAAGG GTCGGTTAGA CATGGATCAG ATGCAAGTGG TGGATGTGGA GGATGGGAAG 
GATAAGGACT TTAACGTGAG TGTGAAGAAT GCCTTGAAAT TATGTTCTCC TGTGGGAGAG 
GAGGTCCACC TTCTGTGTGC CAAGAAACCC GAGCAGAAGC AGCGCTGGCT GCGAGCCTTT 
GCGGATGAAC GGGAACAGGT CCAGCACGAC CTCGAAACAG GTTTTACGAT CACAGAGGTC 
CAGAAGAAGC AGGCCATGTT GAATGCATCT AAAAGTCATC CAACAGGAAA GCCCAAAGCT 
GTGACCAGGC CCTACTATGA CTTCCTGCTG CGTCAGAAGC ACCCCACGCT TCCCACCTCC 
CTGCCCCAGC AGCAGGTCAT CATGTTGGCC GAACCCAAAC GCAAGACCTC CAATTTCTGG 
CACAACATCG GACGCTTGAC ACCGTTCAAA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515440.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370926.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X4, mRNA.

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CloneID	OSi145734
Clone ID Related Accession (Same CDS sequence)	XM_016515440.1
Accession Version	XM_016515440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAACA GAGGCTGTGG CCACAGAGAC ATGGCACAGG CCCCAGCTTT AGAGGACTCT 
GGGAGTGAGG CAGCCTGTCT GGATGATGGG GAGTCTCTGC AGGAGGGGCA TGGAGAGCAA 
CAGGACTATC AGGAATGGTG TGAGCCAGTT TCTGAAGCAG CACAGGGCAC AAAAGAGGAA 
TATTTTGACA CACACTCATG GCACAGTGAC ACTTTCTCTG GCTTGAGTCA TGAAACTAAG 
GACTGCTGCA GGACACCGCT CAGCTCTGTG GGTCATCTGG ACTCACACTC ACCTTCGGTT 
AGAAGGAGAG ATCCCTCTTC ATCAAAACTG CATCATTCTG TCAATCATGC TGACAGTCTC 
AGTAGAACAG AGGATAGAGA ACAATTGCAA AAGCAGACTG AAAATATGGG GATGATGTGC 
ATGGGAGTTG ATGGCACTGA AATGTCTCAA GCTAAGGGTG CTCCGGAATG TGAGGAGGTT 
TTCAGCAATT TTCCAGCGGG ACCTGATGTT GATTCTTCTT CAAGGAATGA CCAATCCTAC 
ATCGTAACAC CCCTACTGGC ATTAGGGAAC GCTGTAGAAC CACAAACACT CACTGAGCCA 
TGTGCTAATG ACTCATTATT GACCTTAACA GACTGTGATG GAGCCAAGTC TCAGTTTGCT 
GACACCTTTG AGAGAAGATC AAAATGGGAT CAAATTAACA AAAATATCAC AGACACTCAG 
TCAGGACACA ATGAGCCGCG AATACAGTTC ATAACCTCAA GTGAGAAGCA GTTACAGCAA 
ACTTATATTG ACAGTAGTAA CACTGAAGTA CAGTTGATTT CGAATGCATC TGTTGCGAAT 
AGTAAAGATA ACGAATGTCG TGAGCTGGGT GTTGATGAAA ATAAGATCAG TTTATCTGTA 
TCACGTCAAC TTTCTGTATA TGAGTCTGTA TCAGTTGAGA AGCCTATTGA ACAGGAATTT 
GAGGAGTCAA CAAAACACAG CCAATCACAG CCCTCAGTAG AGCACAGCAA ATCACAGCCC 
TCAGTAGAGC ACAGCAAATC ACAGCCCTCA GTAGAGCACA GCCAATCACA GGCATCAACA 
GAACAAATCC AATCACAGGA TTCTGTAGAA TGCAGCCAAT CACGGAATTC AGCAGAACAC 
AGGCAATCAC GAGACTCAAT AGAACACAGT CAGTCACAGG ATTCAGTAGA ATGTAGGCAA 
TCACAGAATT CAGTAGAAGA CAGCCAACTA CAAGACTCAG TAGAACATAG TCAGCCAAAG 
GATTTTGTAG AATGCAGCCA ATCACATGAT TCTATAGAAT GCAGCCACTC ACTGTATTCA 
GTAAAACACA GGCAATCACA AGACTCAATA GAACACGGCC AGTCACAGAG CTCAGTAGAA 
TACACCAAAT CACAGGATTC TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACAGA CCCCAGTAGA ACACACCAAA TCACAGGATT CTGTAAAATG CAGTCAATCA 
CAGAATTCAG CAGAACACAA CCAATCACAG GACTCAGTAG AACACAGTCA GTCACAGAGC 
TCAGAAGACA TGCCATCCCA AAATTTCATC AAACACAGCC AATCACAGAC CCCAGTAGAA 
CACCCCAAAT CACAGGATTG TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACGGG ACTCAGTAGA ACACAGCCAG TCACACAGCT CAGTAGAAGA CATGCTATCG 
CAAAATGTAA TTAACCACAA CAAATCACAA GACCCAGTAG AATATACTCT GTCACTGACC 
TCACTACAAC ACACCCAATC ACAGGATTCT ATAGAACTTA GCCCATCAAA TGATTCTGTA 
GAATGCAGCC AATCACAGGA TTCAGTAGAA AACACATTAG AAAGTAGAAA ACGTCAGTCA 
CATACTACAT CAGAACACAT TCAGTTACAG AAGTCTATAG AATGTAGCCA ATCACAGAAT 
TCCATACAAC ACAGGAAATC ACAGGATTTA GCAGAACAAA ACCAATCACA GAACACCATA 
GAGCACAGCC AATCACAAAC TCCAGTGGAA CACAGCCAAT CACAAACTTC AGTGCAACAC 
AGCCAATCAC AAACTTCAGT GGAACACAGC CAATCACAAG TTGATTCCAT TAAGGCAAAT 
TCTATGAATA CCCAGTGGAC TGTGACCGAG TCAGACTCTG GTTTTATTTC AAACACAGCT 
TCTCTTTATG AAAAGGGACA ATTAAAACAG GACTCCGTGG ACGTATGCAT GAATTGTGAA 
GTCAGTCAGT CCTTGAAAAC ATCTGGAAGG CTTTCATCAA AGGATACTGA CAAAGAACTC 
GATTTGAACT TCGAAAAGAA TAACAGTCTA AATTTAAAAA AGCAGAACTT AAATGACACC 
CCAAGTGTTG TTTCTGAATG CGAGTCTTTT GCAGCTGAGA GAGCTTTCTC AAATGTGCCT 
AATGCAACTT CTGCTGGAGT GTCAGATTCA CTGAATCAGC ATGTGATCAA TGACCGGACC 
TCAGAACAGT CAGATCTGCA GGCCAGACCT CATTACGGTC AACAAAATTT CCCAGTTTTA 
CAAATGCACA CTGAAAAAAA GGATTACTCA GTTAGTAGTT ATCACATGGA CAGTCATTGC 
TTAAAGGACA TTGTTTTAGG GGAACATGAG CCTGAGGCAG ATTGTTATGA AACCCGTCAG 
TGCCGTAATA GCAGAATTAA CTGTAACACA CAGGGTGAAT TCACTGAAGC ACAAGTGTAT 
GTTAACCCTA AAACGTTTGA TACCAATGAT GGTGACGTTT TAGAAGGAAC ACAGAAAAAT 
AGCAAAACAC ATCACATCAT TAAAGGGTAC CAGAAAGAAT GTACAATAGT TCCCTCGATA 
GAACAGTCAG TAATTTACTC TGATAGTTCG GGATGCATAG AATACACTAG GCCTAAGTCA 
TTGAGTGTCA CGTGTCCTGA CATGAAGACA GCTGAAGCCG ATGGGTGTCC TGTAGACAGT 
CCTGTCGAAT CTCTGGCTAG TCTGTTTGAG ATTTCGTATG ACAAATCTCA AGTCAGCCCC 
ACAGATGTTT GCTTTGAGCT CAGTGATGGC CTTCATATGA ACTGTAAACA AGTAGCACAT 
TGCATTATAG AATATACAGA GACCTCCAAA CAATTTCACA GACCCGACAC CCTGTCTGAC 
AAACAAGCAA GACAAAACGA GTCAGTTGAA ACAATTGAGC CGGATATTGA TATTTTGTCT 
AGTGCATGTA CTGATCCTTT CACAAATGCA CAATCCCCTT TTGATTATTC CCAGCTGAAC 
AGTAGAAACA ATGAATACAG CTCAGACACA AATAATAATA TTAAAGCCAT TACTTTTGAA 
GAGTGTGCCT CAGGTGAACA GATCTCTTTG CCCAATTCTT TGCAAAAATA CAGCAGGTTT 
AGTATCAATG CTGGATCTTT ACTCTCAAAA GGTGGTGATT GCAAAGACAA TGCCCCTCCT 
AGTCAGGCTA CTCCCTTTAA AGCACCTGAA ATGAGGACTG CAATTGACAG CAAAGATAAT 
GTTGCTAAAC CTTCTGAATT CAATGAATTC AGCAGCCATA GAGATGCTAT ACTAAAGCAG 
CTCTGTGATT GCTGTGGTGG AAAGGAGTGC CAGCTGCCCT CGTCTGCAGT GGAAGGAGAG 
CAGACAGCAG GAGGATTTCA GTTCAAGGGC ACAGACGGCA TCAGTCAGAG CAGAACTGCA 
GTGTTGCAAA CCTTGAGTCC CTCACACACA CATGACCAAT GCAACACACA TGGAGAGGTT 
AATGCAGACT CTACAAACTG TGAATTTTCA CATATTGGCC ATTTGCTTGC ACACAAACAT 
CCGGAGCCCG TCCAAGAGAA AAGCACATTA CCACTGAGTG GCTCACTCAA GGTCGATGAT 
ACTTATGCAT TGGAAAACAT GGAGAGAAAG CTTGAAACTT TCATGGCTAG CTCTCCTGAA 
AGCACTGATT CAATATGTGA AACTTCTGAT CCTCTATGTA AGCAACCTTT GCATCTGCGC 
AACTCGGAGA CCGAGGGTGC ATTTGTTTCA TGTTGCCGTA TTTTACCCAT CACTAATTTA 
GAGGCCATTT TGGAGTTGGA CCGTTCACCG GAGAGCTCAT TTGTGATAGA AAATGGACTG 
GGGGAGCAAC AGGGAGGAAG TCCATTATCA GAAGGTGGAT TCAGTGAGGA ATGGAGCTCT 
TGTGATGACG TCACAAACCG CCCACATGAG AACATTTCTG CCATGTGTAC AAAAGCCAAC 
GTAGCCACTG TCATGAATGA ACAAGATGCT GGAGAAACAA TGAGCTCTTT ATCAAATGGT 
TTTGATAGTG ATGGTTATAG TGTTTCCAGT TTTGTGTCAC AAGACCTTGA CTCCAGTACC 
AACAGTGAAG AGACGGAGGA CAGCACTCAG TCATCATCTA GTCTTCGTGG TTCAAGAAAT 
AAGAGAACCC AATCAGGGAA GGGTTCTGTG TTTTCTGTTT TTTCTCGAAT GCCCTCATTC 
CGCAAGATTA AACGAGAACA CAAAGGCAAT AGCAAGGTTG ATCCAGAAAT TAAAGATTCC 
AATGAGGATG GAGATGAACG GGGTGAGTTG AAGTACAATA GGTCAACCAC ACATAACAAA 
TCCAACCCTT CACTGTACAA TACCTCTTTT TCTCAAAGTA CAGATCATCT TACAGACATT 
ACAAAGTTTG TTGACCACTC CAATGATGAT ATTTTTGAAA AAGCTTTTGC TTTAAACATG 
CAGAATAGAG AGAAGTATAC ACAATCCCAA AATTTGTATC AGCAGCACAA AGATGGAGAT 
GCACTGAATT TTAGAGTTTC CCCAACGACA GAGGGATTGC AACAAAAAAG AAGCAAGAGT 
ACTGATAACT TAAACTTGCG CATGAAGCTC GCAATGGCTC ACAAGTCTCT GTCTAGCCTG 
TTTGAGTCCA GATATCCTGA GAGGGACAAC CAGGAGCAGA TTGCACAGTC AGAGAATGAG 
GAGGTAAAAA CCAAGCAGTC CTGGAGGAAG TCTAAACGGT CTAAGGAAGC AGAACTTTGC 
AAAAGAACTC TATCGGTTCC TGGGATGGTC TGTGACAAGT CCATACATCA GAGCCATATC 
GATTGTGCCT TCTGTGCTCC TCAGAACAGG TCCAGACTTA ATAGCTCACC AGCTTCCCAA 
AAGAGTTTGA GGAGCACGTG CCATTCTGAT TCACAGAGTT TGAAATCTGG TCTTCAGGAA 
CAGTCTGAAG ACAGAAAATA TCTGGAAGGA GGTGAGCTGC CTTGTGTGCC TTACTTAGAT 
TCTGACCAGA TCTCAGAAGA TGGCTTAGAG TCCAGTGTAG AGGATAGGTC GCAATCACCT 
GTACACCAAA CTGTTTTTGC ACTTGCAAAC CAGATGTCAC CTACCTGGAC CAGATCTTTA 
AGCTGTTTTG AGACCACTGA TACGCCAACG AGGCCCATGA GCCCTAAACC TCATAGTCCT 
GGTCTGGGCT GGACACACCG GAGGAGCTTC CGTTACCCTT CGAGATCTGT TGCATCTTCT 
CTCTGCTCAC TCGGTCAAGG AGTGAGCACT GACGGTCTCT CCGATCCACC TCAGAGACCG 
ACGTCTCTGA AACCAAGGAC AGCACAACTT ACAACAACCC ACTCTTTTGA TTCTGAGTCC 
TTACTAGAGG ACAGCAGTTC AGACAGCCAG TCTCAAAATA GCCTGAATTC AGCATCCCTG 
ATCAACAAAC CAGAGCATGT ACAGCACAGT ACTGAGCCAG CCATCCAGTC AGACGAGAGA 
AGACAACAGG GAAGTGCATG TGTGCTTCGG GTGAAGTCCA GACAGCAGGG TAAAGCTGCA 
CCCAGACCCA TCTCAGACCT CTACGGTTGG ACCGTGTCTC TTCAGGGCAT CAGGGAGGTG 
GCGCTTGATC TGGAGAGGAA GAGCAATTCG ACCGAAACCC GTCGGCGTTC ATGCTCATAT 
GAAACGTTGA CTGATGTGGA GAACACCCAT AAAAAGAAGC TTAACACGCG GCAAAGTTTG 
CGTCAGCTTA GCTCACCAAC ATCTCAGAAG CACGAGAAAG TGTGCGCTAG ACTTTCCCTC 
ACTTCTCCAG AGCAGTTCCC CTCTGTTCCT CTCAAAGACC ACTTCTTCTC CCAGAGCACA 
CCTGTTGGAC TGGACTTTTT GGGCTGGCCT CACCGTGCAT CATTTTCAGA ATCGAATCTC 
AGCACACCAG CACAAGAGGC ACATGAACAG GACCCTCCTC TGGCCTTGGT GATAACTGAT 
GGACCGCAGG ATAAGTCTGG CCTTGCGGAT GAGGTGGGGA GTGAAGATGA CCTGTACAAT 
GAGTTTCGCA GCTCCTCCAA CCGATTCGGA CACCCAGGAG GAGGAGGAGA ACAGCTGGCC 
ATAAATGAGC TGATCAGTGA TGGCAGCGTG TGCGCGGAGG CTCTATGGGA CCACGTCACC 
ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGAGACGTCA TCGAAGTAGT GGACGCCACA 
AACAAGGAGT GGTGGTGGGG CCGGGTGCTG GATAGCGAGG GCTGGTTCCC CGCCAGCTTT 
GTTCGGTTAC GGGTGAACCA AGATGAGCCC ATGGAGGAAT ACTTAGCCCA TCTGGACGGG 
GCCTCGGAGG GGGGTGGGGC CAGCATGGGG GACCCCTTAG GGCCCGGTCT GCCCTGCAAG 
GAGCAGATGA GAGCCAACGT CATCAATGAG ATCATGAGCA CAGAGAGGGA TTACATCAAA 
CACCTGAAGG ACATCTGTGA GGGCTATATA AAACAGTGTC GGAAGAGGAC AGATATGTTT 
ACTGAGGAGC AACTGCGCAC TATCTTTGGG AACATCGATG AGCTCTACCG TTTCCAGAAG 
AAGTTTCTCA AAGCCTTGGA GAAGAAATTC AACAAAGACC ATCCTCACCT CAGCGAGATT 
GGCTCCTGCT TCTTAGAGCA TCAAACAAAC TTTCAGATCT ACTCTGAATA TTGCAACAAC 
CACCCCAATG CCTGCGTGCA GCTCTCCAAA CTGATGAAGA TCAAGAAGTA TGTGTTCTTC 
TTTGAGGCCT GTCGCCTGCT CCAGAAGATG ATCGACATTT CCTTGGATGG ATTCCTGCTT 
ACTCCTGTTC AGAAGATCTG CAAGTATCCT CTGCAGCTGG CTGAACTGCT GAAGTACACC 
AACCCACAGC ACAGAGATTA TAAGGATGTA GAAGCTGCCT TAAATGCAAT GAAGAATGTG 
GCCCGACTGA TCAATGAGAG AAAGAGGCGT CTGGAGAATA TTGACAAAAT TGCTCAGTGG 
CAAAGCTCCA TAGAAGACTG GGAGGGTGAA GACATCTTGA GCAGAAGCTC TGATCTGATT 
TTCTCAGGAG ACCTGACCAA GATCTCCCAG CCACAGGGCA AAGGCCAGCA GCGAATGTTC 
TTTCTCTTTG ACCACCAACT GGTTTTCTGT AAGAAGGATC TGCTTCGGAG GGACATCCTG 
TACTACAAGG GTCGGTTAGA CATGGATCAG ATGCAAGTGG TGGATGTGGA GGATGGGAAG 
GATAAGGACT TTAACGTGAG TGTGAAGAAT GCCTTGAAAT TATGTTCTCC TGTGGGAGAG 
GAGGTCCACC TTCTGTGTGC CAAGAAACCC GAGCAGAAGC AGCGCTGGCT GCGAGCCTTT 
GCGGATGAAC GGGAACAGGT CCAGCACGAC CTCGAAACAG GTTTTACGAT CACAGAGGTC 
CAGAAGAAGC AGGCCATGTT GAATGCATCT AAAAGTCATC CAACAGGAAA GCCCAAAGCT 
GTGACCAGGC CCTACTATGA CTTCCTGCTG CGTCAGAAGC ACCCCACGCT TCCCACCTCC 
CTGCCCCAGC AGCAGGTCAT CATGTTGGCC GAACCCAAAC GCAAGACCTC CAATTTCTGG 
CACAACATCG GACGCTTGAC ACCGTTCAAA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370926.1
CDS	67..7962
Translation	MPNRGCGHRDMAQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515440.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515440.1

ORF Insert Sequence:

ATGCCAAACA GAGGCTGTGG CCACAGAGAC ATGGCACAGG CCCCAGCTTT AGAGGACTCT 
GGGAGTGAGG CAGCCTGTCT GGATGATGGG GAGTCTCTGC AGGAGGGGCA TGGAGAGCAA 
CAGGACTATC AGGAATGGTG TGAGCCAGTT TCTGAAGCAG CACAGGGCAC AAAAGAGGAA 
TATTTTGACA CACACTCATG GCACAGTGAC ACTTTCTCTG GCTTGAGTCA TGAAACTAAG 
GACTGCTGCA GGACACCGCT CAGCTCTGTG GGTCATCTGG ACTCACACTC ACCTTCGGTT 
AGAAGGAGAG ATCCCTCTTC ATCAAAACTG CATCATTCTG TCAATCATGC TGACAGTCTC 
AGTAGAACAG AGGATAGAGA ACAATTGCAA AAGCAGACTG AAAATATGGG GATGATGTGC 
ATGGGAGTTG ATGGCACTGA AATGTCTCAA GCTAAGGGTG CTCCGGAATG TGAGGAGGTT 
TTCAGCAATT TTCCAGCGGG ACCTGATGTT GATTCTTCTT CAAGGAATGA CCAATCCTAC 
ATCGTAACAC CCCTACTGGC ATTAGGGAAC GCTGTAGAAC CACAAACACT CACTGAGCCA 
TGTGCTAATG ACTCATTATT GACCTTAACA GACTGTGATG GAGCCAAGTC TCAGTTTGCT 
GACACCTTTG AGAGAAGATC AAAATGGGAT CAAATTAACA AAAATATCAC AGACACTCAG 
TCAGGACACA ATGAGCCGCG AATACAGTTC ATAACCTCAA GTGAGAAGCA GTTACAGCAA 
ACTTATATTG ACAGTAGTAA CACTGAAGTA CAGTTGATTT CGAATGCATC TGTTGCGAAT 
AGTAAAGATA ACGAATGTCG TGAGCTGGGT GTTGATGAAA ATAAGATCAG TTTATCTGTA 
TCACGTCAAC TTTCTGTATA TGAGTCTGTA TCAGTTGAGA AGCCTATTGA ACAGGAATTT 
GAGGAGTCAA CAAAACACAG CCAATCACAG CCCTCAGTAG AGCACAGCAA ATCACAGCCC 
TCAGTAGAGC ACAGCAAATC ACAGCCCTCA GTAGAGCACA GCCAATCACA GGCATCAACA 
GAACAAATCC AATCACAGGA TTCTGTAGAA TGCAGCCAAT CACGGAATTC AGCAGAACAC 
AGGCAATCAC GAGACTCAAT AGAACACAGT CAGTCACAGG ATTCAGTAGA ATGTAGGCAA 
TCACAGAATT CAGTAGAAGA CAGCCAACTA CAAGACTCAG TAGAACATAG TCAGCCAAAG 
GATTTTGTAG AATGCAGCCA ATCACATGAT TCTATAGAAT GCAGCCACTC ACTGTATTCA 
GTAAAACACA GGCAATCACA AGACTCAATA GAACACGGCC AGTCACAGAG CTCAGTAGAA 
TACACCAAAT CACAGGATTC TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACAGA CCCCAGTAGA ACACACCAAA TCACAGGATT CTGTAAAATG CAGTCAATCA 
CAGAATTCAG CAGAACACAA CCAATCACAG GACTCAGTAG AACACAGTCA GTCACAGAGC 
TCAGAAGACA TGCCATCCCA AAATTTCATC AAACACAGCC AATCACAGAC CCCAGTAGAA 
CACCCCAAAT CACAGGATTG TGTAAAATGC AGTCAATCAC AGAATTCAGT AGAACACAAC 
CAATCACGGG ACTCAGTAGA ACACAGCCAG TCACACAGCT CAGTAGAAGA CATGCTATCG 
CAAAATGTAA TTAACCACAA CAAATCACAA GACCCAGTAG AATATACTCT GTCACTGACC 
TCACTACAAC ACACCCAATC ACAGGATTCT ATAGAACTTA GCCCATCAAA TGATTCTGTA 
GAATGCAGCC AATCACAGGA TTCAGTAGAA AACACATTAG AAAGTAGAAA ACGTCAGTCA 
CATACTACAT CAGAACACAT TCAGTTACAG AAGTCTATAG AATGTAGCCA ATCACAGAAT 
TCCATACAAC ACAGGAAATC ACAGGATTTA GCAGAACAAA ACCAATCACA GAACACCATA 
GAGCACAGCC AATCACAAAC TCCAGTGGAA CACAGCCAAT CACAAACTTC AGTGCAACAC 
AGCCAATCAC AAACTTCAGT GGAACACAGC CAATCACAAG TTGATTCCAT TAAGGCAAAT 
TCTATGAATA CCCAGTGGAC TGTGACCGAG TCAGACTCTG GTTTTATTTC AAACACAGCT 
TCTCTTTATG AAAAGGGACA ATTAAAACAG GACTCCGTGG ACGTATGCAT GAATTGTGAA 
GTCAGTCAGT CCTTGAAAAC ATCTGGAAGG CTTTCATCAA AGGATACTGA CAAAGAACTC 
GATTTGAACT TCGAAAAGAA TAACAGTCTA AATTTAAAAA AGCAGAACTT AAATGACACC 
CCAAGTGTTG TTTCTGAATG CGAGTCTTTT GCAGCTGAGA GAGCTTTCTC AAATGTGCCT 
AATGCAACTT CTGCTGGAGT GTCAGATTCA CTGAATCAGC ATGTGATCAA TGACCGGACC 
TCAGAACAGT CAGATCTGCA GGCCAGACCT CATTACGGTC AACAAAATTT CCCAGTTTTA 
CAAATGCACA CTGAAAAAAA GGATTACTCA GTTAGTAGTT ATCACATGGA CAGTCATTGC 
TTAAAGGACA TTGTTTTAGG GGAACATGAG CCTGAGGCAG ATTGTTATGA AACCCGTCAG 
TGCCGTAATA GCAGAATTAA CTGTAACACA CAGGGTGAAT TCACTGAAGC ACAAGTGTAT 
GTTAACCCTA AAACGTTTGA TACCAATGAT GGTGACGTTT TAGAAGGAAC ACAGAAAAAT 
AGCAAAACAC ATCACATCAT TAAAGGGTAC CAGAAAGAAT GTACAATAGT TCCCTCGATA 
GAACAGTCAG TAATTTACTC TGATAGTTCG GGATGCATAG AATACACTAG GCCTAAGTCA 
TTGAGTGTCA CGTGTCCTGA CATGAAGACA GCTGAAGCCG ATGGGTGTCC TGTAGACAGT 
CCTGTCGAAT CTCTGGCTAG TCTGTTTGAG ATTTCGTATG ACAAATCTCA AGTCAGCCCC 
ACAGATGTTT GCTTTGAGCT CAGTGATGGC CTTCATATGA ACTGTAAACA AGTAGCACAT 
TGCATTATAG AATATACAGA GACCTCCAAA CAATTTCACA GACCCGACAC CCTGTCTGAC 
AAACAAGCAA GACAAAACGA GTCAGTTGAA ACAATTGAGC CGGATATTGA TATTTTGTCT 
AGTGCATGTA CTGATCCTTT CACAAATGCA CAATCCCCTT TTGATTATTC CCAGCTGAAC 
AGTAGAAACA ATGAATACAG CTCAGACACA AATAATAATA TTAAAGCCAT TACTTTTGAA 
GAGTGTGCCT CAGGTGAACA GATCTCTTTG CCCAATTCTT TGCAAAAATA CAGCAGGTTT 
AGTATCAATG CTGGATCTTT ACTCTCAAAA GGTGGTGATT GCAAAGACAA TGCCCCTCCT 
AGTCAGGCTA CTCCCTTTAA AGCACCTGAA ATGAGGACTG CAATTGACAG CAAAGATAAT 
GTTGCTAAAC CTTCTGAATT CAATGAATTC AGCAGCCATA GAGATGCTAT ACTAAAGCAG 
CTCTGTGATT GCTGTGGTGG AAAGGAGTGC CAGCTGCCCT CGTCTGCAGT GGAAGGAGAG 
CAGACAGCAG GAGGATTTCA GTTCAAGGGC ACAGACGGCA TCAGTCAGAG CAGAACTGCA 
GTGTTGCAAA CCTTGAGTCC CTCACACACA CATGACCAAT GCAACACACA TGGAGAGGTT 
AATGCAGACT CTACAAACTG TGAATTTTCA CATATTGGCC ATTTGCTTGC ACACAAACAT 
CCGGAGCCCG TCCAAGAGAA AAGCACATTA CCACTGAGTG GCTCACTCAA GGTCGATGAT 
ACTTATGCAT TGGAAAACAT GGAGAGAAAG CTTGAAACTT TCATGGCTAG CTCTCCTGAA 
AGCACTGATT CAATATGTGA AACTTCTGAT CCTCTATGTA AGCAACCTTT GCATCTGCGC 
AACTCGGAGA CCGAGGGTGC ATTTGTTTCA TGTTGCCGTA TTTTACCCAT CACTAATTTA 
GAGGCCATTT TGGAGTTGGA CCGTTCACCG GAGAGCTCAT TTGTGATAGA AAATGGACTG 
GGGGAGCAAC AGGGAGGAAG TCCATTATCA GAAGGTGGAT TCAGTGAGGA ATGGAGCTCT 
TGTGATGACG TCACAAACCG CCCACATGAG AACATTTCTG CCATGTGTAC AAAAGCCAAC 
GTAGCCACTG TCATGAATGA ACAAGATGCT GGAGAAACAA TGAGCTCTTT ATCAAATGGT 
TTTGATAGTG ATGGTTATAG TGTTTCCAGT TTTGTGTCAC AAGACCTTGA CTCCAGTACC 
AACAGTGAAG AGACGGAGGA CAGCACTCAG TCATCATCTA GTCTTCGTGG TTCAAGAAAT 
AAGAGAACCC AATCAGGGAA GGGTTCTGTG TTTTCTGTTT TTTCTCGAAT GCCCTCATTC 
CGCAAGATTA AACGAGAACA CAAAGGCAAT AGCAAGGTTG ATCCAGAAAT TAAAGATTCC 
AATGAGGATG GAGATGAACG GGGTGAGTTG AAGTACAATA GGTCAACCAC ACATAACAAA 
TCCAACCCTT CACTGTACAA TACCTCTTTT TCTCAAAGTA CAGATCATCT TACAGACATT 
ACAAAGTTTG TTGACCACTC CAATGATGAT ATTTTTGAAA AAGCTTTTGC TTTAAACATG 
CAGAATAGAG AGAAGTATAC ACAATCCCAA AATTTGTATC AGCAGCACAA AGATGGAGAT 
GCACTGAATT TTAGAGTTTC CCCAACGACA GAGGGATTGC AACAAAAAAG AAGCAAGAGT 
ACTGATAACT TAAACTTGCG CATGAAGCTC GCAATGGCTC ACAAGTCTCT GTCTAGCCTG 
TTTGAGTCCA GATATCCTGA GAGGGACAAC CAGGAGCAGA TTGCACAGTC AGAGAATGAG 
GAGGTAAAAA CCAAGCAGTC CTGGAGGAAG TCTAAACGGT CTAAGGAAGC AGAACTTTGC 
AAAAGAACTC TATCGGTTCC TGGGATGGTC TGTGACAAGT CCATACATCA GAGCCATATC 
GATTGTGCCT TCTGTGCTCC TCAGAACAGG TCCAGACTTA ATAGCTCACC AGCTTCCCAA 
AAGAGTTTGA GGAGCACGTG CCATTCTGAT TCACAGAGTT TGAAATCTGG TCTTCAGGAA 
CAGTCTGAAG ACAGAAAATA TCTGGAAGGA GGTGAGCTGC CTTGTGTGCC TTACTTAGAT 
TCTGACCAGA TCTCAGAAGA TGGCTTAGAG TCCAGTGTAG AGGATAGGTC GCAATCACCT 
GTACACCAAA CTGTTTTTGC ACTTGCAAAC CAGATGTCAC CTACCTGGAC CAGATCTTTA 
AGCTGTTTTG AGACCACTGA TACGCCAACG AGGCCCATGA GCCCTAAACC TCATAGTCCT 
GGTCTGGGCT GGACACACCG GAGGAGCTTC CGTTACCCTT CGAGATCTGT TGCATCTTCT 
CTCTGCTCAC TCGGTCAAGG AGTGAGCACT GACGGTCTCT CCGATCCACC TCAGAGACCG 
ACGTCTCTGA AACCAAGGAC AGCACAACTT ACAACAACCC ACTCTTTTGA TTCTGAGTCC 
TTACTAGAGG ACAGCAGTTC AGACAGCCAG TCTCAAAATA GCCTGAATTC AGCATCCCTG 
ATCAACAAAC CAGAGCATGT ACAGCACAGT ACTGAGCCAG CCATCCAGTC AGACGAGAGA 
AGACAACAGG GAAGTGCATG TGTGCTTCGG GTGAAGTCCA GACAGCAGGG TAAAGCTGCA 
CCCAGACCCA TCTCAGACCT CTACGGTTGG ACCGTGTCTC TTCAGGGCAT CAGGGAGGTG 
GCGCTTGATC TGGAGAGGAA GAGCAATTCG ACCGAAACCC GTCGGCGTTC ATGCTCATAT 
GAAACGTTGA CTGATGTGGA GAACACCCAT AAAAAGAAGC TTAACACGCG GCAAAGTTTG 
CGTCAGCTTA GCTCACCAAC ATCTCAGAAG CACGAGAAAG TGTGCGCTAG ACTTTCCCTC 
ACTTCTCCAG AGCAGTTCCC CTCTGTTCCT CTCAAAGACC ACTTCTTCTC CCAGAGCACA 
CCTGTTGGAC TGGACTTTTT GGGCTGGCCT CACCGTGCAT CATTTTCAGA ATCGAATCTC 
AGCACACCAG CACAAGAGGC ACATGAACAG GACCCTCCTC TGGCCTTGGT GATAACTGAT 
GGACCGCAGG ATAAGTCTGG CCTTGCGGAT GAGGTGGGGA GTGAAGATGA CCTGTACAAT 
GAGTTTCGCA GCTCCTCCAA CCGATTCGGA CACCCAGGAG GAGGAGGAGA ACAGCTGGCC 
ATAAATGAGC TGATCAGTGA TGGCAGCGTG TGCGCGGAGG CTCTATGGGA CCACGTCACC 
ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGAGACGTCA TCGAAGTAGT GGACGCCACA 
AACAAGGAGT GGTGGTGGGG CCGGGTGCTG GATAGCGAGG GCTGGTTCCC CGCCAGCTTT 
GTTCGGTTAC GGGTGAACCA AGATGAGCCC ATGGAGGAAT ACTTAGCCCA TCTGGACGGG 
GCCTCGGAGG GGGGTGGGGC CAGCATGGGG GACCCCTTAG GGCCCGGTCT GCCCTGCAAG 
GAGCAGATGA GAGCCAACGT CATCAATGAG ATCATGAGCA CAGAGAGGGA TTACATCAAA 
CACCTGAAGG ACATCTGTGA GGGCTATATA AAACAGTGTC GGAAGAGGAC AGATATGTTT 
ACTGAGGAGC AACTGCGCAC TATCTTTGGG AACATCGATG AGCTCTACCG TTTCCAGAAG 
AAGTTTCTCA AAGCCTTGGA GAAGAAATTC AACAAAGACC ATCCTCACCT CAGCGAGATT 
GGCTCCTGCT TCTTAGAGCA TCAAACAAAC TTTCAGATCT ACTCTGAATA TTGCAACAAC 
CACCCCAATG CCTGCGTGCA GCTCTCCAAA CTGATGAAGA TCAAGAAGTA TGTGTTCTTC 
TTTGAGGCCT GTCGCCTGCT CCAGAAGATG ATCGACATTT CCTTGGATGG ATTCCTGCTT 
ACTCCTGTTC AGAAGATCTG CAAGTATCCT CTGCAGCTGG CTGAACTGCT GAAGTACACC 
AACCCACAGC ACAGAGATTA TAAGGATGTA GAAGCTGCCT TAAATGCAAT GAAGAATGTG 
GCCCGACTGA TCAATGAGAG AAAGAGGCGT CTGGAGAATA TTGACAAAAT TGCTCAGTGG 
CAAAGCTCCA TAGAAGACTG GGAGGGTGAA GACATCTTGA GCAGAAGCTC TGATCTGATT 
TTCTCAGGAG ACCTGACCAA GATCTCCCAG CCACAGGGCA AAGGCCAGCA GCGAATGTTC 
TTTCTCTTTG ACCACCAACT GGTTTTCTGT AAGAAGGATC TGCTTCGGAG GGACATCCTG 
TACTACAAGG GTCGGTTAGA CATGGATCAG ATGCAAGTGG TGGATGTGGA GGATGGGAAG 
GATAAGGACT TTAACGTGAG TGTGAAGAAT GCCTTGAAAT TATGTTCTCC TGTGGGAGAG 
GAGGTCCACC TTCTGTGTGC CAAGAAACCC GAGCAGAAGC AGCGCTGGCT GCGAGCCTTT 
GCGGATGAAC GGGAACAGGT CCAGCACGAC CTCGAAACAG GTTTTACGAT CACAGAGGTC 
CAGAAGAAGC AGGCCATGTT GAATGCATCT AAAAGTCATC CAACAGGAAA GCCCAAAGCT 
GTGACCAGGC CCTACTATGA CTTCCTGCTG CGTCAGAAGC ACCCCACGCT TCCCACCTCC 
CTGCCCCAGC AGCAGGTCAT CATGTTGGCC GAACCCAAAC GCAAGACCTC CAATTTCTGG 
CACAACATCG GACGCTTGAC ACCGTTCAAA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515441.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370927.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X5, mRNA.

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CloneID	OSi145735
Clone ID Related Accession (Same CDS sequence)	XM_016515441.1
Accession Version	XM_016515441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGGTGT GCGCTAGACT TTCCCTCACT TCTCCAGAGC AGTTCCCCTC TGTTCCTCTC 
AAAGACCACT TCTTCTCCCA GAGCACACCT GTTGGACTGG ACTTTTTGGG CTGGCCTCAC 
CGTGCATCAT TTTCAGAATC GAATCTCAGC ACACCAGCAC AAGAGGCACA TGAACAGGAC 
CCTCCTCTGG CCTTGGTGAT AACTGATGGA CCGCAGGATA AGTCTGGCCT TGCGGATGAG 
GTGGGGAGTG AAGATGACCT GTACAATGAG TTTCGCAGCT CCTCCAACCG ATTCGGACAC 
CCAGGAGGAG GAGGAGAACA GCTGGCCATA AATGAGCTGA TCAGTGATGG CAGCGTGTGC 
GCGGAGGCTC TATGGGACCA CGTCACCATG GACGACCAGG AGCTGGGCTT CAAAGCTGGA 
GACGTCATCG AAGTAGTGGA CGCCACAAAC AAGGAGTGGT GGTGGGGCCG GGTGCTGGAT 
AGCGAGGGCT GGTTCCCCGC CAGCTTTGTT CGGTTACGGG TGAACCAAGA TGAGCCCATG 
GAGGAATACT TAGCCCATCT GGACGGGGCC TCGGAGGGGG GTGGGGCCAG CATGGGGGAC 
CCCTTAGGGC CCGGTCTGCC CTGCAAGGAG CAGATGAGAG CCAACGTCAT CAATGAGATC 
ATGAGCACAG AGAGGGATTA CATCAAACAC CTGAAGGACA TCTGTGAGGG CTATATAAAA 
CAGTGTCGGA AGAGGACAGA TATGTTTACT GAGGAGCAAC TGCGCACTAT CTTTGGGAAC 
ATCGATGAGC TCTACCGTTT CCAGAAGAAG TTTCTCAAAG CCTTGGAGAA GAAATTCAAC 
AAAGACCATC CTCACCTCAG CGAGATTGGC TCCTGCTTCT TAGAGCATCA AACAAACTTT 
CAGATCTACT CTGAATATTG CAACAACCAC CCCAATGCCT GCGTGCAGCT CTCCAAACTG 
ATGAAGATCA AGAAGTATGT GTTCTTCTTT GAGGCCTGTC GCCTGCTCCA GAAGATGATC 
GACATTTCCT TGGATGGATT CCTGCTTACT CCTGTTCAGA AGATCTGCAA GTATCCTCTG 
CAGCTGGCTG AACTGCTGAA GTACACCAAC CCACAGCACA GAGATTATAA GGATGTAGAA 
GCTGCCTTAA ATGCAATGAA GAATGTGGCC CGACTGATCA ATGAGAGAAA GAGGCGTCTG 
GAGAATATTG ACAAAATTGC TCAGTGGCAA AGCTCCATAG AAGACTGGGA GGGTGAAGAC 
ATCTTGAGCA GAAGCTCTGA TCTGATTTTC TCAGGAGACC TGACCAAGAT CTCCCAGCCA 
CAGGGCAAAG GCCAGCAGCG AATGTTCTTT CTCTTTGACC ACCAACTGGT TTTCTGTAAG 
AAGGATCTGC TTCGGAGGGA CATCCTGTAC TACAAGGGTC GGTTAGACAT GGATCAGATG 
CAAGTGGTGG ATGTGGAGGA TGGGAAGGAT AAGGACTTTA ACGTGAGTGT GAAGAATGCC 
TTGAAATTAT GTTCTCCTGT GGGAGAGGAG GTCCACCTTC TGTGTGCCAA GAAACCCGAG 
CAGAAGCAGC GCTGGCTGCG AGCCTTTGCG GATGAACGGG AACAGGTCCA GCACGACCTC 
GAAACAGGTT TTACGATCAC AGAGGTCCAG AAGAAGCAGG CCATGTTGAA TGCATCTAAA 
AGTCATCCAA CAGGAAAGCC CAAAGCTGTG ACCAGGCCCT ACTATGACTT CCTGCTGCGT 
CAGAAGCACC CCACGCTTCC CACCTCCCTG CCCCAGCAGC AGGTCATCAT GTTGGCCGAA 
CCCAAACGCA AGACCTCCAA TTTCTGGCAC AACATCGGAC GCTTGACACC GTTCAAAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370927.1
CDS	431..8173
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQRLRPSPVLPQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515441.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515441.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGGTGT GCGCTAGACT TTCCCTCACT TCTCCAGAGC AGTTCCCCTC TGTTCCTCTC 
AAAGACCACT TCTTCTCCCA GAGCACACCT GTTGGACTGG ACTTTTTGGG CTGGCCTCAC 
CGTGCATCAT TTTCAGAATC GAATCTCAGC ACACCAGCAC AAGAGGCACA TGAACAGGAC 
CCTCCTCTGG CCTTGGTGAT AACTGATGGA CCGCAGGATA AGTCTGGCCT TGCGGATGAG 
GTGGGGAGTG AAGATGACCT GTACAATGAG TTTCGCAGCT CCTCCAACCG ATTCGGACAC 
CCAGGAGGAG GAGGAGAACA GCTGGCCATA AATGAGCTGA TCAGTGATGG CAGCGTGTGC 
GCGGAGGCTC TATGGGACCA CGTCACCATG GACGACCAGG AGCTGGGCTT CAAAGCTGGA 
GACGTCATCG AAGTAGTGGA CGCCACAAAC AAGGAGTGGT GGTGGGGCCG GGTGCTGGAT 
AGCGAGGGCT GGTTCCCCGC CAGCTTTGTT CGGTTACGGG TGAACCAAGA TGAGCCCATG 
GAGGAATACT TAGCCCATCT GGACGGGGCC TCGGAGGGGG GTGGGGCCAG CATGGGGGAC 
CCCTTAGGGC CCGGTCTGCC CTGCAAGGAG CAGATGAGAG CCAACGTCAT CAATGAGATC 
ATGAGCACAG AGAGGGATTA CATCAAACAC CTGAAGGACA TCTGTGAGGG CTATATAAAA 
CAGTGTCGGA AGAGGACAGA TATGTTTACT GAGGAGCAAC TGCGCACTAT CTTTGGGAAC 
ATCGATGAGC TCTACCGTTT CCAGAAGAAG TTTCTCAAAG CCTTGGAGAA GAAATTCAAC 
AAAGACCATC CTCACCTCAG CGAGATTGGC TCCTGCTTCT TAGAGCATCA AACAAACTTT 
CAGATCTACT CTGAATATTG CAACAACCAC CCCAATGCCT GCGTGCAGCT CTCCAAACTG 
ATGAAGATCA AGAAGTATGT GTTCTTCTTT GAGGCCTGTC GCCTGCTCCA GAAGATGATC 
GACATTTCCT TGGATGGATT CCTGCTTACT CCTGTTCAGA AGATCTGCAA GTATCCTCTG 
CAGCTGGCTG AACTGCTGAA GTACACCAAC CCACAGCACA GAGATTATAA GGATGTAGAA 
GCTGCCTTAA ATGCAATGAA GAATGTGGCC CGACTGATCA ATGAGAGAAA GAGGCGTCTG 
GAGAATATTG ACAAAATTGC TCAGTGGCAA AGCTCCATAG AAGACTGGGA GGGTGAAGAC 
ATCTTGAGCA GAAGCTCTGA TCTGATTTTC TCAGGAGACC TGACCAAGAT CTCCCAGCCA 
CAGGGCAAAG GCCAGCAGCG AATGTTCTTT CTCTTTGACC ACCAACTGGT TTTCTGTAAG 
AAGGATCTGC TTCGGAGGGA CATCCTGTAC TACAAGGGTC GGTTAGACAT GGATCAGATG 
CAAGTGGTGG ATGTGGAGGA TGGGAAGGAT AAGGACTTTA ACGTGAGTGT GAAGAATGCC 
TTGAAATTAT GTTCTCCTGT GGGAGAGGAG GTCCACCTTC TGTGTGCCAA GAAACCCGAG 
CAGAAGCAGC GCTGGCTGCG AGCCTTTGCG GATGAACGGG AACAGGTCCA GCACGACCTC 
GAAACAGGTT TTACGATCAC AGAGGTCCAG AAGAAGCAGG CCATGTTGAA TGCATCTAAA 
AGTCATCCAA CAGGAAAGCC CAAAGCTGTG ACCAGGCCCT ACTATGACTT CCTGCTGCGT 
CAGAAGCACC CCACGCTTCC CACCTCCCTG CCCCAGCAGC AGGTCATCAT GTTGGCCGAA 
CCCAAACGCA AGACCTCCAA TTTCTGGCAC AACATCGGAC GCTTGACACC GTTCAAAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515442.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370928.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X6, mRNA.

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CloneID	OSi145736
Clone ID Related Accession (Same CDS sequence)	XM_016515442.1
Accession Version	XM_016515442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
CTGATCAGTG ATGGCAGCGT GTGCGCGGAG GCTCTATGGG ACCACGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGAGACGTC ATCGAAGTAG TGGACGCCAC AAACAAGGAG 
TGGTGGTGGG GCCGGGTGCT GGATAGCGAG GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA 
CGGGTGAACC AAGATGAGCC CATGGAGGAA TACTTAGCCC ATCTGGACGG GGCCTCGGAG 
GGGGGTGGGG CCAGCATGGG GGACCCCTTA GGGCCCGGTC TGCCCTGCAA GGAGCAGATG 
AGAGCCAACG TCATCAATGA GATCATGAGC ACAGAGAGGG ATTACATCAA ACACCTGAAG 
GACATCTGTG AGGGCTATAT AAAACAGTGT CGGAAGAGGA CAGATATGTT TACTGAGGAG 
CAACTGCGCA CTATCTTTGG GAACATCGAT GAGCTCTACC GTTTCCAGAA GAAGTTTCTC 
AAAGCCTTGG AGAAGAAATT CAACAAAGAC CATCCTCACC TCAGCGAGAT TGGCTCCTGC 
TTCTTAGAGC ATCAAACAAA CTTTCAGATC TACTCTGAAT ATTGCAACAA CCACCCCAAT 
GCCTGCGTGC AGCTCTCCAA ACTGATGAAG ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC 
TGTCGCCTGC TCCAGAAGAT GATCGACATT TCCTTGGATG GATTCCTGCT TACTCCTGTT 
CAGAAGATCT GCAAGTATCC TCTGCAGCTG GCTGAACTGC TGAAGTACAC CAACCCACAG 
CACAGAGATT ATAAGGATGT AGAAGCTGCC TTAAATGCAA TGAAGAATGT GGCCCGACTG 
ATCAATGAGA GAAAGAGGCG TCTGGAGAAT ATTGACAAAA TTGCTCAGTG GCAAAGCTCC 
ATAGAAGACT GGGAGGGTGA AGACATCTTG AGCAGAAGCT CTGATCTGAT TTTCTCAGGA 
GACCTGACCA AGATCTCCCA GCCACAGGGC AAAGGCCAGC AGCGAATGTT CTTTCTCTTT 
GACCACCAAC TGGTTTTCTG TAAGAAGGAT CTGCTTCGGA GGGACATCCT GTACTACAAG 
GGTCGGTTAG ACATGGATCA GATGCAAGTG GTGGATGTGG AGGATGGGAA GGATAAGGAC 
TTTAACGTTT TACGATCACA GAGGTCCAGA AGAAGCAGGC CATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370928.1
CDS	432..8159
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQRLRPSPVLPQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVLRSQRSRRSRPC

Target ORF information:

RefSeq Version	XM_016515442.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515442.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
CTGATCAGTG ATGGCAGCGT GTGCGCGGAG GCTCTATGGG ACCACGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGAGACGTC ATCGAAGTAG TGGACGCCAC AAACAAGGAG 
TGGTGGTGGG GCCGGGTGCT GGATAGCGAG GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA 
CGGGTGAACC AAGATGAGCC CATGGAGGAA TACTTAGCCC ATCTGGACGG GGCCTCGGAG 
GGGGGTGGGG CCAGCATGGG GGACCCCTTA GGGCCCGGTC TGCCCTGCAA GGAGCAGATG 
AGAGCCAACG TCATCAATGA GATCATGAGC ACAGAGAGGG ATTACATCAA ACACCTGAAG 
GACATCTGTG AGGGCTATAT AAAACAGTGT CGGAAGAGGA CAGATATGTT TACTGAGGAG 
CAACTGCGCA CTATCTTTGG GAACATCGAT GAGCTCTACC GTTTCCAGAA GAAGTTTCTC 
AAAGCCTTGG AGAAGAAATT CAACAAAGAC CATCCTCACC TCAGCGAGAT TGGCTCCTGC 
TTCTTAGAGC ATCAAACAAA CTTTCAGATC TACTCTGAAT ATTGCAACAA CCACCCCAAT 
GCCTGCGTGC AGCTCTCCAA ACTGATGAAG ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC 
TGTCGCCTGC TCCAGAAGAT GATCGACATT TCCTTGGATG GATTCCTGCT TACTCCTGTT 
CAGAAGATCT GCAAGTATCC TCTGCAGCTG GCTGAACTGC TGAAGTACAC CAACCCACAG 
CACAGAGATT ATAAGGATGT AGAAGCTGCC TTAAATGCAA TGAAGAATGT GGCCCGACTG 
ATCAATGAGA GAAAGAGGCG TCTGGAGAAT ATTGACAAAA TTGCTCAGTG GCAAAGCTCC 
ATAGAAGACT GGGAGGGTGA AGACATCTTG AGCAGAAGCT CTGATCTGAT TTTCTCAGGA 
GACCTGACCA AGATCTCCCA GCCACAGGGC AAAGGCCAGC AGCGAATGTT CTTTCTCTTT 
GACCACCAAC TGGTTTTCTG TAAGAAGGAT CTGCTTCGGA GGGACATCCT GTACTACAAG 
GGTCGGTTAG ACATGGATCA GATGCAAGTG GTGGATGTGG AGGATGGGAA GGATAAGGAC 
TTTAACGTTT TACGATCACA GAGGTCCAGA AGAAGCAGGC CATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515443.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370929.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X7, mRNA.

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CloneID	OSi145737
Clone ID Related Accession (Same CDS sequence)	XM_016515443.1
Accession Version	XM_016515443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATGA TGTGCATGGG AGTTGATGGC ACTGAAATGT CTCAAGCTAA GGGTGCTCCG 
GAATGTGAGG AGGTTTTCAG CAATTTTCCA GCGGGACCTG ATGTTGATTC TTCTTCAAGG 
AATGACCAAT CCTACATCGT AACACCCCTA CTGGCATTAG GGAACGCTGT AGAACCACAA 
ACACTCACTG AGCCATGTGC TAATGACTCA TTATTGACCT TAACAGACTG TGATGGAGCC 
AAGTCTCAGT TTGCTGACAC CTTTGAGAGA AGATCAAAAT GGGATCAAAT TAACAAAAAT 
ATCACAGACA CTCAGTCAGG ACACAATGAG CCGCGAATAC AGTTCATAAC CTCAAGTGAG 
AAGCAGTTAC AGCAAACTTA TATTGACAGT AGTAACACTG AAGTACAGTT GATTTCGAAT 
GCATCTGTTG CGAATAGTAA AGATAACGAA TGTCGTGAGC TGGGTGTTGA TGAAAATAAG 
ATCAGTTTAT CTGTATCACG TCAACTTTCT GTATATGAGT CTGTATCAGT TGAGAAGCCT 
ATTGAACAGG AATTTGAGGA GTCAACAAAA CACAGCCAAT CACAGCCCTC AGTAGAGCAC 
AGCAAATCAC AGCCCTCAGT AGAGCACAGC AAATCACAGC CCTCAGTAGA GCACAGCCAA 
TCACAGGCAT CAACAGAACA AATCCAATCA CAGGATTCTG TAGAATGCAG CCAATCACGG 
AATTCAGCAG AACACAGGCA ATCACGAGAC TCAATAGAAC ACAGTCAGTC ACAGGATTCA 
GTAGAATGTA GGCAATCACA GAATTCAGTA GAAGACAGCC AACTACAAGA CTCAGTAGAA 
CATAGTCAGC CAAAGGATTT TGTAGAATGC AGCCAATCAC ATGATTCTAT AGAATGCAGC 
CACTCACTGT ATTCAGTAAA ACACAGGCAA TCACAAGACT CAATAGAACA CGGCCAGTCA 
CAGAGCTCAG TAGAATACAC CAAATCACAG GATTCTGTAA AATGCAGTCA ATCACAGAAT 
TCAGTAGAAC ACAACCAATC ACAGACCCCA GTAGAACACA CCAAATCACA GGATTCTGTA 
AAATGCAGTC AATCACAGAA TTCAGCAGAA CACAACCAAT CACAGGACTC AGTAGAACAC 
AGTCAGTCAC AGAGCTCAGA AGACATGCCA TCCCAAAATT TCATCAAACA CAGCCAATCA 
CAGACCCCAG TAGAACACCC CAAATCACAG GATTGTGTAA AATGCAGTCA ATCACAGAAT 
TCAGTAGAAC ACAACCAATC ACGGGACTCA GTAGAACACA GCCAGTCACA CAGCTCAGTA 
GAAGACATGC TATCGCAAAA TGTAATTAAC CACAACAAAT CACAAGACCC AGTAGAATAT 
ACTCTGTCAC TGACCTCACT ACAACACACC CAATCACAGG ATTCTATAGA ACTTAGCCCA 
TCAAATGATT CTGTAGAATG CAGCCAATCA CAGGATTCAG TAGAAAACAC ATTAGAAAGT 
AGAAAACGTC AGTCACATAC TACATCAGAA CACATTCAGT TACAGAAGTC TATAGAATGT 
AGCCAATCAC AGAATTCCAT ACAACACAGG AAATCACAGG ATTTAGCAGA ACAAAACCAA 
TCACAGAACA CCATAGAGCA CAGCCAATCA CAAACTCCAG TGGAACACAG CCAATCACAA 
ACTTCAGTGC AACACAGCCA ATCACAAACT TCAGTGGAAC ACAGCCAATC ACAAGTTGAT 
TCCATTAAGG CAAATTCTAT GAATACCCAG TGGACTGTGA CCGAGTCAGA CTCTGGTTTT 
ATTTCAAACA CAGCTTCTCT TTATGAAAAG GGACAATTAA AACAGGACTC CGTGGACGTA 
TGCATGAATT GTGAAGTCAG TCAGTCCTTG AAAACATCTG GAAGGCTTTC ATCAAAGGAT 
ACTGACAAAG AACTCGATTT GAACTTCGAA AAGAATAACA GTCTAAATTT AAAAAAGCAG 
AACTTAAATG ACACCCCAAG TGTTGTTTCT GAATGCGAGT CTTTTGCAGC TGAGAGAGCT 
TTCTCAAATG TGCCTAATGC AACTTCTGCT GGAGTGTCAG ATTCACTGAA TCAGCATGTG 
ATCAATGACC GGACCTCAGA ACAGTCAGAT CTGCAGGCCA GACCTCATTA CGGTCAACAA 
AATTTCCCAG TTTTACAAAT GCACACTGAA AAAAAGGATT ACTCAGTTAG TAGTTATCAC 
ATGGACAGTC ATTGCTTAAA GGACATTGTT TTAGGGGAAC ATGAGCCTGA GGCAGATTGT 
TATGAAACCC GTCAGTGCCG TAATAGCAGA ATTAACTGTA ACACACAGGG TGAATTCACT 
GAAGCACAAG TGTATGTTAA CCCTAAAACG TTTGATACCA ATGATGGTGA CGTTTTAGAA 
GGAACACAGA AAAATAGCAA AACACATCAC ATCATTAAAG GGTACCAGAA AGAATGTACA 
ATAGTTCCCT CGATAGAACA GTCAGTAATT TACTCTGATA GTTCGGGATG CATAGAATAC 
ACTAGGCCTA AGTCATTGAG TGTCACGTGT CCTGACATGA AGACAGCTGA AGCCGATGGG 
TGTCCTGTAG ACAGTCCTGT CGAATCTCTG GCTAGTCTGT TTGAGATTTC GTATGACAAA 
TCTCAAGTCA GCCCCACAGA TGTTTGCTTT GAGCTCAGTG ATGGCCTTCA TATGAACTGT 
AAACAAGTAG CACATTGCAT TATAGAATAT ACAGAGACCT CCAAACAATT TCACAGACCC 
GACACCCTGT CTGACAAACA AGCAAGACAA AACGAGTCAG TTGAAACAAT TGAGCCGGAT 
ATTGATATTT TGTCTAGTGC ATGTACTGAT CCTTTCACAA ATGCACAATC CCCTTTTGAT 
TATTCCCAGC TGAACAGTAG AAACAATGAA TACAGCTCAG ACACAAATAA TAATATTAAA 
GCCATTACTT TTGAAGAGTG TGCCTCAGGT GAACAGATCT CTTTGCCCAA TTCTTTGCAA 
AAATACAGCA GGTTTAGTAT CAATGCTGGA TCTTTACTCT CAAAAGGTGG TGATTGCAAA 
GACAATGCCC CTCCTAGTCA GGCTACTCCC TTTAAAGCAC CTGAAATGAG GACTGCAATT 
GACAGCAAAG ATAATGTTGC TAAACCTTCT GAATTCAATG AATTCAGCAG CCATAGAGAT 
GCTATACTAA AGCAGCTCTG TGATTGCTGT GGTGGAAAGG AGTGCCAGCT GCCCTCGTCT 
GCAGTGGAAG GAGAGCAGAC AGCAGGAGGA TTTCAGTTCA AGGGCACAGA CGGCATCAGT 
CAGAGCAGAA CTGCAGTGTT GCAAACCTTG AGTCCCTCAC ACACACATGA CCAATGCAAC 
ACACATGGAG AGGTTAATGC AGACTCTACA AACTGTGAAT TTTCACATAT TGGCCATTTG 
CTTGCACACA AACATCCGGA GCCCGTCCAA GAGAAAAGCA CATTACCACT GAGTGGCTCA 
CTCAAGGTCG ATGATACTTA TGCATTGGAA AACATGGAGA GAAAGCTTGA AACTTTCATG 
GCTAGCTCTC CTGAAAGCAC TGATTCAATA TGTGAAACTT CTGATCCTCT ATGTAAGCAA 
CCTTTGCATC TGCGCAACTC GGAGACCGAG GGTGCATTTG TTTCATGTTG CCGTATTTTA 
CCCATCACTA ATTTAGAGGC CATTTTGGAG TTGGACCGTT CACCGGAGAG CTCATTTGTG 
ATAGAAAATG GACTGGGGGA GCAACAGGGA GGAAGTCCAT TATCAGAAGG TGGATTCAGT 
GAGGAATGGA GCTCTTGTGA TGACGTCACA AACCGCCCAC ATGAGAACAT TTCTGCCATG 
TGTACAAAAG CCAACGTAGC CACTGTCATG AATGAACAAG ATGCTGGAGA AACAATGAGC 
TCTTTATCAA ATGGTTTTGA TAGTGATGGT TATAGTGTTT CCAGTTTTGT GTCACAAGAC 
CTTGACTCCA GTACCAACAG TGAAGAGACG GAGGACAGCA CTCAGTCATC ATCTAGTCTT 
CGTGGTTCAA GAAATAAGAG AACCCAATCA GGGAAGGGTT CTGTGTTTTC TGTTTTTTCT 
CGAATGCCCT CATTCCGCAA GATTAAACGA GAACACAAAG GCAATAGCAA GGTTGATCCA 
GAAATTAAAG ATTCCAATGA GGATGGAGAT GAACGGGGTG AGTTGAAGTA CAATAGGTCA 
ACCACACATA ACAAATCCAA CCCTTCACTG TACAATACCT CTTTTTCTCA AAGTACAGAT 
CATCTTACAG ACATTACAAA GTTTGTTGAC CACTCCAATG ATGATATTTT TGAAAAAGCT 
TTTGCTTTAA ACATGCAGAA TAGAGAGAAG TATACACAAT CCCAAAATTT GTATCAGCAG 
CACAAAGATG GAGATGCACT GAATTTTAGA GTTTCCCCAA CGACAGAGGG ATTGCAACAA 
AAAAGAAGCA AGAGTACTGA TAACTTAAAC TTGCGCATGA AGCTCGCAAT GGCTCACAAG 
TCTCTGTCTA GCCTGTTTGA GTCCAGATAT CCTGAGAGGG ACAACCAGGA GCAGATTGCA 
CAGTCAGAGA ATGAGGAGGT AAAAACCAAG CAGTCCTGGA GGAAGTCTAA ACGGTCTAAG 
GAAGCAGAAC TTTGCAAAAG AACTCTATCG GTTCCTGGGA TGGTCTGTGA CAAGTCCATA 
CATCAGAGCC ATATCGATTG TGCCTTCTGT GCTCCTCAGA ACAGGTCCAG ACTTAATAGC 
TCACCAGCTT CCCAAAAGAG TTTGAGGAGC ACGTGCCATT CTGATTCACA GAGTTTGAAA 
TCTGGTCTTC AGGAACAGTC TGAAGACAGA AAATATCTGG AAGGAGGTGA GCTGCCTTGT 
GTGCCTTACT TAGATTCTGA CCAGATCTCA GAAGATGGCT TAGAGTCCAG TGTAGAGGAT 
AGGTCGCAAT CACCTGTACA CCAAACTGTT TTTGCACTTG CAAACCAGAT GTCACCTACC 
TGGACCAGAT CTTTAAGCTG TTTTGAGACC ACTGATACGC CAACGAGGCC CATGAGCCCT 
AAACCTCATA GTCCTGGTCT GGGCTGGACA CACCGGAGGA GCTTCCGTTA CCCTTCGAGA 
TCTGTTGCAT CTTCTCTCTG CTCACTCGGT CAAGGAGTGA GCACTGACGG TCTCTCCGAT 
CCACCTCAGA GACCGACGTC TCTGAAACCA AGGACAGCAC AACTTACAAC AACCCACTCT 
TTTGATTCTG AGTCCTTACT AGAGGACAGC AGTTCAGACA GCCAGTCTCA AAATAGCCTG 
AATTCAGCAT CCCTGATCAA CAAACCAGAG CATGTACAGC ACAGTACTGA GCCAGCCATC 
CAGTCAGACG AGAGAAGACA ACAGGGAAGT GCATGTGTGC TTCGGGTGAA GTCCAGACAG 
CAGGGTAAAG CTGCACCCAG ACCCATCTCA GACCTCTACG GTTGGACCGT GTCTCTTCAG 
GGCATCAGGG AGGTGGCGCT TGATCTGGAG AGGAAGAGCA ATTCGACCGA AACCCGTCGG 
CGTTCATGCT CATATGAAAC GTTGACTGAT GTGGAGAACA CCCATAAAAA GAAGCTTAAC 
ACGCGGCAAA GTTTGCGTCA GCTTAGCTCA CCAACATCTC AGAAGCACGA GAAAGTGTGC 
GCTAGACTTT CCCTCACTTC TCCAGAGCAG TTCCCCTCTG TTCCTCTCAA AGACCACTTC 
TTCTCCCAGA GCACACCTGT TGGACTGGAC TTTTTGGGCT GGCCTCACCG TGCATCATTT 
TCAGAATCGA ATCTCAGCAC ACCAGCACAA GAGGCACATG AACAGGACCC TCCTCTGGCC 
TTGGTGATAA CTGATGGACC GCAGGATAAG TCTGGCCTTG CGGATGAGGT GGGGAGTGAA 
GATGACCTGT ACAATGAGTT TCGCAGCTCC TCCAACCGAT TCGGACACCC AGGAGGAGGA 
GGAGAACAGC TGGCCATAAA TGAGCTGATC AGTGATGGCA GCGTGTGCGC GGAGGCTCTA 
TGGGACCACG TCACCATGGA CGACCAGGAG CTGGGCTTCA AAGCTGGAGA CGTCATCGAA 
GTAGTGGACG CCACAAACAA GGAGTGGTGG TGGGGCCGGG TGCTGGATAG CGAGGGCTGG 
TTCCCCGCCA GCTTTGTTCG GTTACGGGTG AACCAAGATG AGCCCATGGA GGAATACTTA 
GCCCATCTGG ACGGGGCCTC GGAGGGGGGT GGGGCCAGCA TGGGGGACCC CTTAGGGCCC 
GGTCTGCCCT GCAAGGAGCA GATGAGAGCC AACGTCATCA ATGAGATCAT GAGCACAGAG 
AGGGATTACA TCAAACACCT GAAGGACATC TGTGAGGGCT ATATAAAACA GTGTCGGAAG 
AGGACAGATA TGTTTACTGA GGAGCAACTG CGCACTATCT TTGGGAACAT CGATGAGCTC 
TACCGTTTCC AGAAGAAGTT TCTCAAAGCC TTGGAGAAGA AATTCAACAA AGACCATCCT 
CACCTCAGCG AGATTGGCTC CTGCTTCTTA GAGCATCAAA CAAACTTTCA GATCTACTCT 
GAATATTGCA ACAACCACCC CAATGCCTGC GTGCAGCTCT CCAAACTGAT GAAGATCAAG 
AAGTATGTGT TCTTCTTTGA GGCCTGTCGC CTGCTCCAGA AGATGATCGA CATTTCCTTG 
GATGGATTCC TGCTTACTCC TGTTCAGAAG ATCTGCAAGT ATCCTCTGCA GCTGGCTGAA 
CTGCTGAAGT ACACCAACCC ACAGCACAGA GATTATAAGG ATGTAGAAGC TGCCTTAAAT 
GCAATGAAGA ATGTGGCCCG ACTGATCAAT GAGAGAAAGA GGCGTCTGGA GAATATTGAC 
AAAATTGCTC AGTGGCAAAG CTCCATAGAA GACTGGGAGG GTGAAGACAT CTTGAGCAGA 
AGCTCTGATC TGATTTTCTC AGGAGACCTG ACCAAGATCT CCCAGCCACA GGGCAAAGGC 
CAGCAGCGAA TGTTCTTTCT CTTTGACCAC CAACTGGTTT TCTGTAAGAA GGATCTGCTT 
CGGAGGGACA TCCTGTACTA CAAGGGTCGG TTAGACATGG ATCAGATGCA AGTGGTGGAT 
GTGGAGGATG GGAAGGATAA GGACTTTAAC GTGAGTGTGA AGAATGCCTT GAAATTATGT 
TCTCCTGTGG GAGAGGAGGT CCACCTTCTG TGTGCCAAGA AACCCGAGCA GAAGCAGCGC 
TGGCTGCGAG CCTTTGCGGA TGAACGGGAA CAGGTCCAGC ACGACCTCGA AACAGGTTTT 
ACGATCACAG AGGTCCAGAA GAAGCAGGCC ATGTTGAATG CATCTAAAAG TCATCCAACA 
GGAAAGCCCA AAGCTGTGAC CAGGCCCTAC TATGACTTCC TGCTGCGTCA GAAGCACCCC 
ACGCTTCCCA CCTCCCTGCC CCAGCAGCAG GTCATCATGT TGGCCGAACC CAAACGCAAG 
ACCTCCAATT TCTGGCACAA CATCGGACGC TTGACACCGT TCAAAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370929.1
CDS	419..7909
Translation	MGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKAVTRPYYDFLLRQKHPTLPTSLPQQQVIMLAEPKRKTSNFWHNIGRLTPFKK

Target ORF information:

RefSeq Version	XM_016515443.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515443.1

ORF Insert Sequence:

ATGGGGATGA TGTGCATGGG AGTTGATGGC ACTGAAATGT CTCAAGCTAA GGGTGCTCCG 
GAATGTGAGG AGGTTTTCAG CAATTTTCCA GCGGGACCTG ATGTTGATTC TTCTTCAAGG 
AATGACCAAT CCTACATCGT AACACCCCTA CTGGCATTAG GGAACGCTGT AGAACCACAA 
ACACTCACTG AGCCATGTGC TAATGACTCA TTATTGACCT TAACAGACTG TGATGGAGCC 
AAGTCTCAGT TTGCTGACAC CTTTGAGAGA AGATCAAAAT GGGATCAAAT TAACAAAAAT 
ATCACAGACA CTCAGTCAGG ACACAATGAG CCGCGAATAC AGTTCATAAC CTCAAGTGAG 
AAGCAGTTAC AGCAAACTTA TATTGACAGT AGTAACACTG AAGTACAGTT GATTTCGAAT 
GCATCTGTTG CGAATAGTAA AGATAACGAA TGTCGTGAGC TGGGTGTTGA TGAAAATAAG 
ATCAGTTTAT CTGTATCACG TCAACTTTCT GTATATGAGT CTGTATCAGT TGAGAAGCCT 
ATTGAACAGG AATTTGAGGA GTCAACAAAA CACAGCCAAT CACAGCCCTC AGTAGAGCAC 
AGCAAATCAC AGCCCTCAGT AGAGCACAGC AAATCACAGC CCTCAGTAGA GCACAGCCAA 
TCACAGGCAT CAACAGAACA AATCCAATCA CAGGATTCTG TAGAATGCAG CCAATCACGG 
AATTCAGCAG AACACAGGCA ATCACGAGAC TCAATAGAAC ACAGTCAGTC ACAGGATTCA 
GTAGAATGTA GGCAATCACA GAATTCAGTA GAAGACAGCC AACTACAAGA CTCAGTAGAA 
CATAGTCAGC CAAAGGATTT TGTAGAATGC AGCCAATCAC ATGATTCTAT AGAATGCAGC 
CACTCACTGT ATTCAGTAAA ACACAGGCAA TCACAAGACT CAATAGAACA CGGCCAGTCA 
CAGAGCTCAG TAGAATACAC CAAATCACAG GATTCTGTAA AATGCAGTCA ATCACAGAAT 
TCAGTAGAAC ACAACCAATC ACAGACCCCA GTAGAACACA CCAAATCACA GGATTCTGTA 
AAATGCAGTC AATCACAGAA TTCAGCAGAA CACAACCAAT CACAGGACTC AGTAGAACAC 
AGTCAGTCAC AGAGCTCAGA AGACATGCCA TCCCAAAATT TCATCAAACA CAGCCAATCA 
CAGACCCCAG TAGAACACCC CAAATCACAG GATTGTGTAA AATGCAGTCA ATCACAGAAT 
TCAGTAGAAC ACAACCAATC ACGGGACTCA GTAGAACACA GCCAGTCACA CAGCTCAGTA 
GAAGACATGC TATCGCAAAA TGTAATTAAC CACAACAAAT CACAAGACCC AGTAGAATAT 
ACTCTGTCAC TGACCTCACT ACAACACACC CAATCACAGG ATTCTATAGA ACTTAGCCCA 
TCAAATGATT CTGTAGAATG CAGCCAATCA CAGGATTCAG TAGAAAACAC ATTAGAAAGT 
AGAAAACGTC AGTCACATAC TACATCAGAA CACATTCAGT TACAGAAGTC TATAGAATGT 
AGCCAATCAC AGAATTCCAT ACAACACAGG AAATCACAGG ATTTAGCAGA ACAAAACCAA 
TCACAGAACA CCATAGAGCA CAGCCAATCA CAAACTCCAG TGGAACACAG CCAATCACAA 
ACTTCAGTGC AACACAGCCA ATCACAAACT TCAGTGGAAC ACAGCCAATC ACAAGTTGAT 
TCCATTAAGG CAAATTCTAT GAATACCCAG TGGACTGTGA CCGAGTCAGA CTCTGGTTTT 
ATTTCAAACA CAGCTTCTCT TTATGAAAAG GGACAATTAA AACAGGACTC CGTGGACGTA 
TGCATGAATT GTGAAGTCAG TCAGTCCTTG AAAACATCTG GAAGGCTTTC ATCAAAGGAT 
ACTGACAAAG AACTCGATTT GAACTTCGAA AAGAATAACA GTCTAAATTT AAAAAAGCAG 
AACTTAAATG ACACCCCAAG TGTTGTTTCT GAATGCGAGT CTTTTGCAGC TGAGAGAGCT 
TTCTCAAATG TGCCTAATGC AACTTCTGCT GGAGTGTCAG ATTCACTGAA TCAGCATGTG 
ATCAATGACC GGACCTCAGA ACAGTCAGAT CTGCAGGCCA GACCTCATTA CGGTCAACAA 
AATTTCCCAG TTTTACAAAT GCACACTGAA AAAAAGGATT ACTCAGTTAG TAGTTATCAC 
ATGGACAGTC ATTGCTTAAA GGACATTGTT TTAGGGGAAC ATGAGCCTGA GGCAGATTGT 
TATGAAACCC GTCAGTGCCG TAATAGCAGA ATTAACTGTA ACACACAGGG TGAATTCACT 
GAAGCACAAG TGTATGTTAA CCCTAAAACG TTTGATACCA ATGATGGTGA CGTTTTAGAA 
GGAACACAGA AAAATAGCAA AACACATCAC ATCATTAAAG GGTACCAGAA AGAATGTACA 
ATAGTTCCCT CGATAGAACA GTCAGTAATT TACTCTGATA GTTCGGGATG CATAGAATAC 
ACTAGGCCTA AGTCATTGAG TGTCACGTGT CCTGACATGA AGACAGCTGA AGCCGATGGG 
TGTCCTGTAG ACAGTCCTGT CGAATCTCTG GCTAGTCTGT TTGAGATTTC GTATGACAAA 
TCTCAAGTCA GCCCCACAGA TGTTTGCTTT GAGCTCAGTG ATGGCCTTCA TATGAACTGT 
AAACAAGTAG CACATTGCAT TATAGAATAT ACAGAGACCT CCAAACAATT TCACAGACCC 
GACACCCTGT CTGACAAACA AGCAAGACAA AACGAGTCAG TTGAAACAAT TGAGCCGGAT 
ATTGATATTT TGTCTAGTGC ATGTACTGAT CCTTTCACAA ATGCACAATC CCCTTTTGAT 
TATTCCCAGC TGAACAGTAG AAACAATGAA TACAGCTCAG ACACAAATAA TAATATTAAA 
GCCATTACTT TTGAAGAGTG TGCCTCAGGT GAACAGATCT CTTTGCCCAA TTCTTTGCAA 
AAATACAGCA GGTTTAGTAT CAATGCTGGA TCTTTACTCT CAAAAGGTGG TGATTGCAAA 
GACAATGCCC CTCCTAGTCA GGCTACTCCC TTTAAAGCAC CTGAAATGAG GACTGCAATT 
GACAGCAAAG ATAATGTTGC TAAACCTTCT GAATTCAATG AATTCAGCAG CCATAGAGAT 
GCTATACTAA AGCAGCTCTG TGATTGCTGT GGTGGAAAGG AGTGCCAGCT GCCCTCGTCT 
GCAGTGGAAG GAGAGCAGAC AGCAGGAGGA TTTCAGTTCA AGGGCACAGA CGGCATCAGT 
CAGAGCAGAA CTGCAGTGTT GCAAACCTTG AGTCCCTCAC ACACACATGA CCAATGCAAC 
ACACATGGAG AGGTTAATGC AGACTCTACA AACTGTGAAT TTTCACATAT TGGCCATTTG 
CTTGCACACA AACATCCGGA GCCCGTCCAA GAGAAAAGCA CATTACCACT GAGTGGCTCA 
CTCAAGGTCG ATGATACTTA TGCATTGGAA AACATGGAGA GAAAGCTTGA AACTTTCATG 
GCTAGCTCTC CTGAAAGCAC TGATTCAATA TGTGAAACTT CTGATCCTCT ATGTAAGCAA 
CCTTTGCATC TGCGCAACTC GGAGACCGAG GGTGCATTTG TTTCATGTTG CCGTATTTTA 
CCCATCACTA ATTTAGAGGC CATTTTGGAG TTGGACCGTT CACCGGAGAG CTCATTTGTG 
ATAGAAAATG GACTGGGGGA GCAACAGGGA GGAAGTCCAT TATCAGAAGG TGGATTCAGT 
GAGGAATGGA GCTCTTGTGA TGACGTCACA AACCGCCCAC ATGAGAACAT TTCTGCCATG 
TGTACAAAAG CCAACGTAGC CACTGTCATG AATGAACAAG ATGCTGGAGA AACAATGAGC 
TCTTTATCAA ATGGTTTTGA TAGTGATGGT TATAGTGTTT CCAGTTTTGT GTCACAAGAC 
CTTGACTCCA GTACCAACAG TGAAGAGACG GAGGACAGCA CTCAGTCATC ATCTAGTCTT 
CGTGGTTCAA GAAATAAGAG AACCCAATCA GGGAAGGGTT CTGTGTTTTC TGTTTTTTCT 
CGAATGCCCT CATTCCGCAA GATTAAACGA GAACACAAAG GCAATAGCAA GGTTGATCCA 
GAAATTAAAG ATTCCAATGA GGATGGAGAT GAACGGGGTG AGTTGAAGTA CAATAGGTCA 
ACCACACATA ACAAATCCAA CCCTTCACTG TACAATACCT CTTTTTCTCA AAGTACAGAT 
CATCTTACAG ACATTACAAA GTTTGTTGAC CACTCCAATG ATGATATTTT TGAAAAAGCT 
TTTGCTTTAA ACATGCAGAA TAGAGAGAAG TATACACAAT CCCAAAATTT GTATCAGCAG 
CACAAAGATG GAGATGCACT GAATTTTAGA GTTTCCCCAA CGACAGAGGG ATTGCAACAA 
AAAAGAAGCA AGAGTACTGA TAACTTAAAC TTGCGCATGA AGCTCGCAAT GGCTCACAAG 
TCTCTGTCTA GCCTGTTTGA GTCCAGATAT CCTGAGAGGG ACAACCAGGA GCAGATTGCA 
CAGTCAGAGA ATGAGGAGGT AAAAACCAAG CAGTCCTGGA GGAAGTCTAA ACGGTCTAAG 
GAAGCAGAAC TTTGCAAAAG AACTCTATCG GTTCCTGGGA TGGTCTGTGA CAAGTCCATA 
CATCAGAGCC ATATCGATTG TGCCTTCTGT GCTCCTCAGA ACAGGTCCAG ACTTAATAGC 
TCACCAGCTT CCCAAAAGAG TTTGAGGAGC ACGTGCCATT CTGATTCACA GAGTTTGAAA 
TCTGGTCTTC AGGAACAGTC TGAAGACAGA AAATATCTGG AAGGAGGTGA GCTGCCTTGT 
GTGCCTTACT TAGATTCTGA CCAGATCTCA GAAGATGGCT TAGAGTCCAG TGTAGAGGAT 
AGGTCGCAAT CACCTGTACA CCAAACTGTT TTTGCACTTG CAAACCAGAT GTCACCTACC 
TGGACCAGAT CTTTAAGCTG TTTTGAGACC ACTGATACGC CAACGAGGCC CATGAGCCCT 
AAACCTCATA GTCCTGGTCT GGGCTGGACA CACCGGAGGA GCTTCCGTTA CCCTTCGAGA 
TCTGTTGCAT CTTCTCTCTG CTCACTCGGT CAAGGAGTGA GCACTGACGG TCTCTCCGAT 
CCACCTCAGA GACCGACGTC TCTGAAACCA AGGACAGCAC AACTTACAAC AACCCACTCT 
TTTGATTCTG AGTCCTTACT AGAGGACAGC AGTTCAGACA GCCAGTCTCA AAATAGCCTG 
AATTCAGCAT CCCTGATCAA CAAACCAGAG CATGTACAGC ACAGTACTGA GCCAGCCATC 
CAGTCAGACG AGAGAAGACA ACAGGGAAGT GCATGTGTGC TTCGGGTGAA GTCCAGACAG 
CAGGGTAAAG CTGCACCCAG ACCCATCTCA GACCTCTACG GTTGGACCGT GTCTCTTCAG 
GGCATCAGGG AGGTGGCGCT TGATCTGGAG AGGAAGAGCA ATTCGACCGA AACCCGTCGG 
CGTTCATGCT CATATGAAAC GTTGACTGAT GTGGAGAACA CCCATAAAAA GAAGCTTAAC 
ACGCGGCAAA GTTTGCGTCA GCTTAGCTCA CCAACATCTC AGAAGCACGA GAAAGTGTGC 
GCTAGACTTT CCCTCACTTC TCCAGAGCAG TTCCCCTCTG TTCCTCTCAA AGACCACTTC 
TTCTCCCAGA GCACACCTGT TGGACTGGAC TTTTTGGGCT GGCCTCACCG TGCATCATTT 
TCAGAATCGA ATCTCAGCAC ACCAGCACAA GAGGCACATG AACAGGACCC TCCTCTGGCC 
TTGGTGATAA CTGATGGACC GCAGGATAAG TCTGGCCTTG CGGATGAGGT GGGGAGTGAA 
GATGACCTGT ACAATGAGTT TCGCAGCTCC TCCAACCGAT TCGGACACCC AGGAGGAGGA 
GGAGAACAGC TGGCCATAAA TGAGCTGATC AGTGATGGCA GCGTGTGCGC GGAGGCTCTA 
TGGGACCACG TCACCATGGA CGACCAGGAG CTGGGCTTCA AAGCTGGAGA CGTCATCGAA 
GTAGTGGACG CCACAAACAA GGAGTGGTGG TGGGGCCGGG TGCTGGATAG CGAGGGCTGG 
TTCCCCGCCA GCTTTGTTCG GTTACGGGTG AACCAAGATG AGCCCATGGA GGAATACTTA 
GCCCATCTGG ACGGGGCCTC GGAGGGGGGT GGGGCCAGCA TGGGGGACCC CTTAGGGCCC 
GGTCTGCCCT GCAAGGAGCA GATGAGAGCC AACGTCATCA ATGAGATCAT GAGCACAGAG 
AGGGATTACA TCAAACACCT GAAGGACATC TGTGAGGGCT ATATAAAACA GTGTCGGAAG 
AGGACAGATA TGTTTACTGA GGAGCAACTG CGCACTATCT TTGGGAACAT CGATGAGCTC 
TACCGTTTCC AGAAGAAGTT TCTCAAAGCC TTGGAGAAGA AATTCAACAA AGACCATCCT 
CACCTCAGCG AGATTGGCTC CTGCTTCTTA GAGCATCAAA CAAACTTTCA GATCTACTCT 
GAATATTGCA ACAACCACCC CAATGCCTGC GTGCAGCTCT CCAAACTGAT GAAGATCAAG 
AAGTATGTGT TCTTCTTTGA GGCCTGTCGC CTGCTCCAGA AGATGATCGA CATTTCCTTG 
GATGGATTCC TGCTTACTCC TGTTCAGAAG ATCTGCAAGT ATCCTCTGCA GCTGGCTGAA 
CTGCTGAAGT ACACCAACCC ACAGCACAGA GATTATAAGG ATGTAGAAGC TGCCTTAAAT 
GCAATGAAGA ATGTGGCCCG ACTGATCAAT GAGAGAAAGA GGCGTCTGGA GAATATTGAC 
AAAATTGCTC AGTGGCAAAG CTCCATAGAA GACTGGGAGG GTGAAGACAT CTTGAGCAGA 
AGCTCTGATC TGATTTTCTC AGGAGACCTG ACCAAGATCT CCCAGCCACA GGGCAAAGGC 
CAGCAGCGAA TGTTCTTTCT CTTTGACCAC CAACTGGTTT TCTGTAAGAA GGATCTGCTT 
CGGAGGGACA TCCTGTACTA CAAGGGTCGG TTAGACATGG ATCAGATGCA AGTGGTGGAT 
GTGGAGGATG GGAAGGATAA GGACTTTAAC GTGAGTGTGA AGAATGCCTT GAAATTATGT 
TCTCCTGTGG GAGAGGAGGT CCACCTTCTG TGTGCCAAGA AACCCGAGCA GAAGCAGCGC 
TGGCTGCGAG CCTTTGCGGA TGAACGGGAA CAGGTCCAGC ACGACCTCGA AACAGGTTTT 
ACGATCACAG AGGTCCAGAA GAAGCAGGCC ATGTTGAATG CATCTAAAAG TCATCCAACA 
GGAAAGCCCA AAGCTGTGAC CAGGCCCTAC TATGACTTCC TGCTGCGTCA GAAGCACCCC 
ACGCTTCCCA CCTCCCTGCC CCAGCAGCAG GTCATCATGT TGGCCGAACC CAAACGCAAG 
ACCTCCAATT TCTGGCACAA CATCGGACGC TTGACACCGT TCAAAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515444.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370930.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X8, mRNA.

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CloneID	OSi145738
Clone ID Related Accession (Same CDS sequence)	XM_016515444.1
Accession Version	XM_016515444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
AGGCTGTTTC TGATCATCTT TGAACAAACC AAGAAGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370930.1
CDS	432..7013
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQRLRPSPVLPQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINERLFLIIFEQTKKR

Target ORF information:

RefSeq Version	XM_016515444.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515444.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
AGGCTGTTTC TGATCATCTT TGAACAAACC AAGAAGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515445.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370931.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X9, mRNA.

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CloneID	OSi145739
Clone ID Related Accession (Same CDS sequence)	XM_016515445.1
Accession Version	XM_016515445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370931.1
CDS	432..6977
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQRLRPSPVLPQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINEA

Target ORF information:

RefSeq Version	XM_016515445.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515445.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107710620 ( XM_016515448.1 ) cDNA ORF clone, Sinocyclocheilus rhinocerous -> XP_016370934.1 Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X12, mRNA.

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CloneID	OSi145742
Clone ID Related Accession (Same CDS sequence)	XM_016515448.1
Accession Version	XM_016515448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus rhinocerous
Product	uncharacterized protein LOC107710620 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015649561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus rhinocerous Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
CTGATCAGTG ATGGCAGCGT GTGCGCGGAG GCTCTATGGG ACCACGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGAGACGTC ATCGAAGTAG TGGACGCCAC AAACAAGGAG 
TGGTGGTGGG GCCGGGTGCT GGATAGCGAG GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA 
CGGGTGAACC AAGATGAGCC CATGGAGGAA TACTTAGCCC ATCTGGACGG GGCCTCGGAG 
GGGGGTGGGG CCAGCATGGG GGACCCCTTA GGGCCCGGTC TGCCCTGCAA GGAGCAGATG 
AGAGCCAACG TCATCAATGA GATCATGAGC ACAGAGAGGG ATTACATCAA ACACCTGAAG 
GACATCTGTG AGGGCTATAT AAAACAGTGT CGGAAGAGGA CAGATATGTT TACTGAGGAG 
CAACTGCGCA CTATCTTTGG GAACATCGAT GAGCTCTACC GTTTCCAGAA GAAGTTTCTC 
AAAGCCTTGG AGAAGAAATT CAACAAAGAC CATCCTCACC TCAGCGAGAT TGGCTCCTGC 
TTCTTAGAGC ATCAAACAAA CTTTCAGATC TACTCTGAAT ATTGCAACAA CCACCCCAAT 
GCCTGCGTGC AGCTCTCCAA ACTGATGAAG ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC 
TGTCGCCTGC TCCAGAAGAT GATCGACATT TCCTTGGATG GATTCCTGCT TACTCCTGTT 
CAGAAGATCT GCAAGTATCC TCTGCAGCTG GCTGAACTGC TGAAGTACAC CAACCCACAG 
CACAGAGATT ATAAGGATGT AGAAGCTGCC TTAAATGCAA TGAAGAATGT GGCCCGACTG 
ATCAATGAGA GAAAGAGGCG TCTGGAGAAT ATTGACAAAA TTGCTCAGTG GCAAAGCTCC 
ATAGAAGACT GGGAGGGTGA AGACATCTTG AGCAGAAGCT CTGATCTGAT TTTCTCAGGA 
GACCTGACCA AGATCTCCCA GCCACAGGGC AAAGGCCAGC AGCGAATGTT CTTTCTCTTT 
GACCACCAAC TGGTTTTCTG TAAGAAGGAT CTGCTTCGGA GGGACATCCT GTACTACAAG 
GGTCGGTTAG ACATGGATCA GATGCAAGTG GTGGATGTGG AGGATGGGAA GGATAAGGAC 
TTTAACGTGA GTGTGAAGAA TGCCTTGAAA TTATGTTCTC CTGTGGGAGA GGAGGTCCAC 
CTTCTGTGTG CCAAGAAACC CGAGCAGAAG CAGCGCTGGC TGCGAGCCTT TGCGGATGAA 
CGGGAACAGG TCCAGCACGA CCTCGAAACA GGTTTTACGA TCACAGAGGT CCAGAAGAAG 
CAGGCCATGT TGAATGCATC TAAAAGTCAT CCAACAGGAA AGCCCAAAGC ATTAGCGTTA 
CAGCATCTTT TGGCACTCAG AACTCCTTCA CTTCTTCACC CAAAAACTTC CAAGTGCCTA 
CGTTTTTCTG AACCTTCAGG ACCTCTGGCT CCCAGGGCCA CCTTAACTTC ACCTCTCCTC 
CCTGAAGGCC CTCAGCGTAG GCGGGGGCGG GGGGTTAGGG TGCCCCCTGG GGTCCTGCGC 
AGGGCAATAT CTTGGGTGTC CTCTCAAAGT AGAGAGCCGA TCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016370934.1
CDS	432..8579
Translation	MEETGAGNGTGTQGCELERSGDGGLDEVQMHLACLPERYMRAKFSLSAYIFCWTVVKLCQRLRPSPVLPQAPALEDSGSEAACLDDGESLQEGHGEQQDYQEWCEPVSEAAQGTKEEYFDTHSWHSDTFSGLSHETKDCCRTPLSSVGHLDSHSPSVRRRDPSSSKLHHSVNHADSLSRTEDREQLQKQTENMGMMCMGVDGTEMSQAKGAPECEEVFSNFPAGPDVDSSSRNDQSYIVTPLLALGNAVEPQTLTEPCANDSLLTLTDCDGAKSQFADTFERRSKWDQINKNITDTQSGHNEPRIQFITSSEKQLQQTYIDSSNTEVQLISNASVANSKDNECRELGVDENKISLSVSRQLSVYESVSVEKPIEQEFEESTKHSQSQPSVEHSKSQPSVEHSKSQPSVEHSQSQASTEQIQSQDSVECSQSRNSAEHRQSRDSIEHSQSQDSVECRQSQNSVEDSQLQDSVEHSQPKDFVECSQSHDSIECSHSLYSVKHRQSQDSIEHGQSQSSVEYTKSQDSVKCSQSQNSVEHNQSQTPVEHTKSQDSVKCSQSQNSAEHNQSQDSVEHSQSQSSEDMPSQNFIKHSQSQTPVEHPKSQDCVKCSQSQNSVEHNQSRDSVEHSQSHSSVEDMLSQNVINHNKSQDPVEYTLSLTSLQHTQSQDSIELSPSNDSVECSQSQDSVENTLESRKRQSHTTSEHIQLQKSIECSQSQNSIQHRKSQDLAEQNQSQNTIEHSQSQTPVEHSQSQTSVQHSQSQTSVEHSQSQVDSIKANSMNTQWTVTESDSGFISNTASLYEKGQLKQDSVDVCMNCEVSQSLKTSGRLSSKDTDKELDLNFEKNNSLNLKKQNLNDTPSVVSECESFAAERAFSNVPNATSAGVSDSLNQHVINDRTSEQSDLQARPHYGQQNFPVLQMHTEKKDYSVSSYHMDSHCLKDIVLGEHEPEADCYETRQCRNSRINCNTQGEFTEAQVYVNPKTFDTNDGDVLEGTQKNSKTHHIIKGYQKECTIVPSIEQSVIYSDSSGCIEYTRPKSLSVTCPDMKTAEADGCPVDSPVESLASLFEISYDKSQVSPTDVCFELSDGLHMNCKQVAHCIIEYTETSKQFHRPDTLSDKQARQNESVETIEPDIDILSSACTDPFTNAQSPFDYSQLNSRNNEYSSDTNNNIKAITFEECASGEQISLPNSLQKYSRFSINAGSLLSKGGDCKDNAPPSQATPFKAPEMRTAIDSKDNVAKPSEFNEFSSHRDAILKQLCDCCGGKECQLPSSAVEGEQTAGGFQFKGTDGISQSRTAVLQTLSPSHTHDQCNTHGEVNADSTNCEFSHIGHLLAHKHPEPVQEKSTLPLSGSLKVDDTYALENMERKLETFMASSPESTDSICETSDPLCKQPLHLRNSETEGAFVSCCRILPITNLEAILELDRSPESSFVIENGLGEQQGGSPLSEGGFSEEWSSCDDVTNRPHENISAMCTKANVATVMNEQDAGETMSSLSNGFDSDGYSVSSFVSQDLDSSTNSEETEDSTQSSSSLRGSRNKRTQSGKGSVFSVFSRMPSFRKIKREHKGNSKVDPEIKDSNEDGDERGELKYNRSTTHNKSNPSLYNTSFSQSTDHLTDITKFVDHSNDDIFEKAFALNMQNREKYTQSQNLYQQHKDGDALNFRVSPTTEGLQQKRSKSTDNLNLRMKLAMAHKSLSSLFESRYPERDNQEQIAQSENEEVKTKQSWRKSKRSKEAELCKRTLSVPGMVCDKSIHQSHIDCAFCAPQNRSRLNSSPASQKSLRSTCHSDSQSLKSGLQEQSEDRKYLEGGELPCVPYLDSDQISEDGLESSVEDRSQSPVHQTVFALANQMSPTWTRSLSCFETTDTPTRPMSPKPHSPGLGWTHRRSFRYPSRSVASSLCSLGQGVSTDGLSDPPQRPTSLKPRTAQLTTTHSFDSESLLEDSSSDSQSQNSLNSASLINKPEHVQHSTEPAIQSDERRQQGSACVLRVKSRQQGKAAPRPISDLYGWTVSLQGIREVALDLERKSNSTETRRRSCSYETLTDVENTHKKKLNTRQSLRQLSSPTSQKHEKVCARLSLTSPEQFPSVPLKDHFFSQSTPVGLDFLGWPHRASFSESNLSTPAQEAHEQDPPLALVITDGPQDKSGLADEVGSEDDLYNEFRSSSNRFGHPGGGGEQLAINELISDGSVCAEALWDHVTMDDQELGFKAGDVIEVVDATNKEWWWGRVLDSEGWFPASFVRLRVNQDEPMEEYLAHLDGASEGGGASMGDPLGPGLPCKEQMRANVINEIMSTERDYIKHLKDICEGYIKQCRKRTDMFTEEQLRTIFGNIDELYRFQKKFLKALEKKFNKDHPHLSEIGSCFLEHQTNFQIYSEYCNNHPNACVQLSKLMKIKKYVFFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTNPQHRDYKDVEAALNAMKNVARLINERKRRLENIDKIAQWQSSIEDWEGEDILSRSSDLIFSGDLTKISQPQGKGQQRMFFLFDHQLVFCKKDLLRRDILYYKGRLDMDQMQVVDVEDGKDKDFNVSVKNALKLCSPVGEEVHLLCAKKPEQKQRWLRAFADEREQVQHDLETGFTITEVQKKQAMLNASKSHPTGKPKALALQHLLALRTPSLLHPKTSKCLRFSEPSGPLAPRATLTSPLLPEGPQRRRGRGVRVPPGVLRRAISWVSSQSREPIE

Target ORF information:

RefSeq Version	XM_016515448.1
Organism	Sinocyclocheilus rhinocerous
Definition	Sinocyclocheilus rhinocerous rho guanine nucleotide exchange factor 4-like (LOC107710620), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016515448.1

ORF Insert Sequence:

ATGGAAGAGA CAGGCGCTGG GAACGGTACT GGGACGCAGG GCTGTGAACT GGAGCGTTCT 
GGAGACGGAG GACTGGATGA GGTGCAGATG CATCTCGCCT GCCTTCCGGA GCGATACATG 
AGAGCGAAGT TCTCCCTCTC GGCGTACATA TTTTGTTGGA CAGTAGTAAA GTTGTGTCAG 
AGATTAAGAC CATCGCCAGT TCTTCCCCAG GCCCCAGCTT TAGAGGACTC TGGGAGTGAG 
GCAGCCTGTC TGGATGATGG GGAGTCTCTG CAGGAGGGGC ATGGAGAGCA ACAGGACTAT 
CAGGAATGGT GTGAGCCAGT TTCTGAAGCA GCACAGGGCA CAAAAGAGGA ATATTTTGAC 
ACACACTCAT GGCACAGTGA CACTTTCTCT GGCTTGAGTC ATGAAACTAA GGACTGCTGC 
AGGACACCGC TCAGCTCTGT GGGTCATCTG GACTCACACT CACCTTCGGT TAGAAGGAGA 
GATCCCTCTT CATCAAAACT GCATCATTCT GTCAATCATG CTGACAGTCT CAGTAGAACA 
GAGGATAGAG AACAATTGCA AAAGCAGACT GAAAATATGG GGATGATGTG CATGGGAGTT 
GATGGCACTG AAATGTCTCA AGCTAAGGGT GCTCCGGAAT GTGAGGAGGT TTTCAGCAAT 
TTTCCAGCGG GACCTGATGT TGATTCTTCT TCAAGGAATG ACCAATCCTA CATCGTAACA 
CCCCTACTGG CATTAGGGAA CGCTGTAGAA CCACAAACAC TCACTGAGCC ATGTGCTAAT 
GACTCATTAT TGACCTTAAC AGACTGTGAT GGAGCCAAGT CTCAGTTTGC TGACACCTTT 
GAGAGAAGAT CAAAATGGGA TCAAATTAAC AAAAATATCA CAGACACTCA GTCAGGACAC 
AATGAGCCGC GAATACAGTT CATAACCTCA AGTGAGAAGC AGTTACAGCA AACTTATATT 
GACAGTAGTA ACACTGAAGT ACAGTTGATT TCGAATGCAT CTGTTGCGAA TAGTAAAGAT 
AACGAATGTC GTGAGCTGGG TGTTGATGAA AATAAGATCA GTTTATCTGT ATCACGTCAA 
CTTTCTGTAT ATGAGTCTGT ATCAGTTGAG AAGCCTATTG AACAGGAATT TGAGGAGTCA 
ACAAAACACA GCCAATCACA GCCCTCAGTA GAGCACAGCA AATCACAGCC CTCAGTAGAG 
CACAGCAAAT CACAGCCCTC AGTAGAGCAC AGCCAATCAC AGGCATCAAC AGAACAAATC 
CAATCACAGG ATTCTGTAGA ATGCAGCCAA TCACGGAATT CAGCAGAACA CAGGCAATCA 
CGAGACTCAA TAGAACACAG TCAGTCACAG GATTCAGTAG AATGTAGGCA ATCACAGAAT 
TCAGTAGAAG ACAGCCAACT ACAAGACTCA GTAGAACATA GTCAGCCAAA GGATTTTGTA 
GAATGCAGCC AATCACATGA TTCTATAGAA TGCAGCCACT CACTGTATTC AGTAAAACAC 
AGGCAATCAC AAGACTCAAT AGAACACGGC CAGTCACAGA GCTCAGTAGA ATACACCAAA 
TCACAGGATT CTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACAG 
ACCCCAGTAG AACACACCAA ATCACAGGAT TCTGTAAAAT GCAGTCAATC ACAGAATTCA 
GCAGAACACA ACCAATCACA GGACTCAGTA GAACACAGTC AGTCACAGAG CTCAGAAGAC 
ATGCCATCCC AAAATTTCAT CAAACACAGC CAATCACAGA CCCCAGTAGA ACACCCCAAA 
TCACAGGATT GTGTAAAATG CAGTCAATCA CAGAATTCAG TAGAACACAA CCAATCACGG 
GACTCAGTAG AACACAGCCA GTCACACAGC TCAGTAGAAG ACATGCTATC GCAAAATGTA 
ATTAACCACA ACAAATCACA AGACCCAGTA GAATATACTC TGTCACTGAC CTCACTACAA 
CACACCCAAT CACAGGATTC TATAGAACTT AGCCCATCAA ATGATTCTGT AGAATGCAGC 
CAATCACAGG ATTCAGTAGA AAACACATTA GAAAGTAGAA AACGTCAGTC ACATACTACA 
TCAGAACACA TTCAGTTACA GAAGTCTATA GAATGTAGCC AATCACAGAA TTCCATACAA 
CACAGGAAAT CACAGGATTT AGCAGAACAA AACCAATCAC AGAACACCAT AGAGCACAGC 
CAATCACAAA CTCCAGTGGA ACACAGCCAA TCACAAACTT CAGTGCAACA CAGCCAATCA 
CAAACTTCAG TGGAACACAG CCAATCACAA GTTGATTCCA TTAAGGCAAA TTCTATGAAT 
ACCCAGTGGA CTGTGACCGA GTCAGACTCT GGTTTTATTT CAAACACAGC TTCTCTTTAT 
GAAAAGGGAC AATTAAAACA GGACTCCGTG GACGTATGCA TGAATTGTGA AGTCAGTCAG 
TCCTTGAAAA CATCTGGAAG GCTTTCATCA AAGGATACTG ACAAAGAACT CGATTTGAAC 
TTCGAAAAGA ATAACAGTCT AAATTTAAAA AAGCAGAACT TAAATGACAC CCCAAGTGTT 
GTTTCTGAAT GCGAGTCTTT TGCAGCTGAG AGAGCTTTCT CAAATGTGCC TAATGCAACT 
TCTGCTGGAG TGTCAGATTC ACTGAATCAG CATGTGATCA ATGACCGGAC CTCAGAACAG 
TCAGATCTGC AGGCCAGACC TCATTACGGT CAACAAAATT TCCCAGTTTT ACAAATGCAC 
ACTGAAAAAA AGGATTACTC AGTTAGTAGT TATCACATGG ACAGTCATTG CTTAAAGGAC 
ATTGTTTTAG GGGAACATGA GCCTGAGGCA GATTGTTATG AAACCCGTCA GTGCCGTAAT 
AGCAGAATTA ACTGTAACAC ACAGGGTGAA TTCACTGAAG CACAAGTGTA TGTTAACCCT 
AAAACGTTTG ATACCAATGA TGGTGACGTT TTAGAAGGAA CACAGAAAAA TAGCAAAACA 
CATCACATCA TTAAAGGGTA CCAGAAAGAA TGTACAATAG TTCCCTCGAT AGAACAGTCA 
GTAATTTACT CTGATAGTTC GGGATGCATA GAATACACTA GGCCTAAGTC ATTGAGTGTC 
ACGTGTCCTG ACATGAAGAC AGCTGAAGCC GATGGGTGTC CTGTAGACAG TCCTGTCGAA 
TCTCTGGCTA GTCTGTTTGA GATTTCGTAT GACAAATCTC AAGTCAGCCC CACAGATGTT 
TGCTTTGAGC TCAGTGATGG CCTTCATATG AACTGTAAAC AAGTAGCACA TTGCATTATA 
GAATATACAG AGACCTCCAA ACAATTTCAC AGACCCGACA CCCTGTCTGA CAAACAAGCA 
AGACAAAACG AGTCAGTTGA AACAATTGAG CCGGATATTG ATATTTTGTC TAGTGCATGT 
ACTGATCCTT TCACAAATGC ACAATCCCCT TTTGATTATT CCCAGCTGAA CAGTAGAAAC 
AATGAATACA GCTCAGACAC AAATAATAAT ATTAAAGCCA TTACTTTTGA AGAGTGTGCC 
TCAGGTGAAC AGATCTCTTT GCCCAATTCT TTGCAAAAAT ACAGCAGGTT TAGTATCAAT 
GCTGGATCTT TACTCTCAAA AGGTGGTGAT TGCAAAGACA ATGCCCCTCC TAGTCAGGCT 
ACTCCCTTTA AAGCACCTGA AATGAGGACT GCAATTGACA GCAAAGATAA TGTTGCTAAA 
CCTTCTGAAT TCAATGAATT CAGCAGCCAT AGAGATGCTA TACTAAAGCA GCTCTGTGAT 
TGCTGTGGTG GAAAGGAGTG CCAGCTGCCC TCGTCTGCAG TGGAAGGAGA GCAGACAGCA 
GGAGGATTTC AGTTCAAGGG CACAGACGGC ATCAGTCAGA GCAGAACTGC AGTGTTGCAA 
ACCTTGAGTC CCTCACACAC ACATGACCAA TGCAACACAC ATGGAGAGGT TAATGCAGAC 
TCTACAAACT GTGAATTTTC ACATATTGGC CATTTGCTTG CACACAAACA TCCGGAGCCC 
GTCCAAGAGA AAAGCACATT ACCACTGAGT GGCTCACTCA AGGTCGATGA TACTTATGCA 
TTGGAAAACA TGGAGAGAAA GCTTGAAACT TTCATGGCTA GCTCTCCTGA AAGCACTGAT 
TCAATATGTG AAACTTCTGA TCCTCTATGT AAGCAACCTT TGCATCTGCG CAACTCGGAG 
ACCGAGGGTG CATTTGTTTC ATGTTGCCGT ATTTTACCCA TCACTAATTT AGAGGCCATT 
TTGGAGTTGG ACCGTTCACC GGAGAGCTCA TTTGTGATAG AAAATGGACT GGGGGAGCAA 
CAGGGAGGAA GTCCATTATC AGAAGGTGGA TTCAGTGAGG AATGGAGCTC TTGTGATGAC 
GTCACAAACC GCCCACATGA GAACATTTCT GCCATGTGTA CAAAAGCCAA CGTAGCCACT 
GTCATGAATG AACAAGATGC TGGAGAAACA ATGAGCTCTT TATCAAATGG TTTTGATAGT 
GATGGTTATA GTGTTTCCAG TTTTGTGTCA CAAGACCTTG ACTCCAGTAC CAACAGTGAA 
GAGACGGAGG ACAGCACTCA GTCATCATCT AGTCTTCGTG GTTCAAGAAA TAAGAGAACC 
CAATCAGGGA AGGGTTCTGT GTTTTCTGTT TTTTCTCGAA TGCCCTCATT CCGCAAGATT 
AAACGAGAAC ACAAAGGCAA TAGCAAGGTT GATCCAGAAA TTAAAGATTC CAATGAGGAT 
GGAGATGAAC GGGGTGAGTT GAAGTACAAT AGGTCAACCA CACATAACAA ATCCAACCCT 
TCACTGTACA ATACCTCTTT TTCTCAAAGT ACAGATCATC TTACAGACAT TACAAAGTTT 
GTTGACCACT CCAATGATGA TATTTTTGAA AAAGCTTTTG CTTTAAACAT GCAGAATAGA 
GAGAAGTATA CACAATCCCA AAATTTGTAT CAGCAGCACA AAGATGGAGA TGCACTGAAT 
TTTAGAGTTT CCCCAACGAC AGAGGGATTG CAACAAAAAA GAAGCAAGAG TACTGATAAC 
TTAAACTTGC GCATGAAGCT CGCAATGGCT CACAAGTCTC TGTCTAGCCT GTTTGAGTCC 
AGATATCCTG AGAGGGACAA CCAGGAGCAG ATTGCACAGT CAGAGAATGA GGAGGTAAAA 
ACCAAGCAGT CCTGGAGGAA GTCTAAACGG TCTAAGGAAG CAGAACTTTG CAAAAGAACT 
CTATCGGTTC CTGGGATGGT CTGTGACAAG TCCATACATC AGAGCCATAT CGATTGTGCC 
TTCTGTGCTC CTCAGAACAG GTCCAGACTT AATAGCTCAC CAGCTTCCCA AAAGAGTTTG 
AGGAGCACGT GCCATTCTGA TTCACAGAGT TTGAAATCTG GTCTTCAGGA ACAGTCTGAA 
GACAGAAAAT ATCTGGAAGG AGGTGAGCTG CCTTGTGTGC CTTACTTAGA TTCTGACCAG 
ATCTCAGAAG ATGGCTTAGA GTCCAGTGTA GAGGATAGGT CGCAATCACC TGTACACCAA 
ACTGTTTTTG CACTTGCAAA CCAGATGTCA CCTACCTGGA CCAGATCTTT AAGCTGTTTT 
GAGACCACTG ATACGCCAAC GAGGCCCATG AGCCCTAAAC CTCATAGTCC TGGTCTGGGC 
TGGACACACC GGAGGAGCTT CCGTTACCCT TCGAGATCTG TTGCATCTTC TCTCTGCTCA 
CTCGGTCAAG GAGTGAGCAC TGACGGTCTC TCCGATCCAC CTCAGAGACC GACGTCTCTG 
AAACCAAGGA CAGCACAACT TACAACAACC CACTCTTTTG ATTCTGAGTC CTTACTAGAG 
GACAGCAGTT CAGACAGCCA GTCTCAAAAT AGCCTGAATT CAGCATCCCT GATCAACAAA 
CCAGAGCATG TACAGCACAG TACTGAGCCA GCCATCCAGT CAGACGAGAG AAGACAACAG 
GGAAGTGCAT GTGTGCTTCG GGTGAAGTCC AGACAGCAGG GTAAAGCTGC ACCCAGACCC 
ATCTCAGACC TCTACGGTTG GACCGTGTCT CTTCAGGGCA TCAGGGAGGT GGCGCTTGAT 
CTGGAGAGGA AGAGCAATTC GACCGAAACC CGTCGGCGTT CATGCTCATA TGAAACGTTG 
ACTGATGTGG AGAACACCCA TAAAAAGAAG CTTAACACGC GGCAAAGTTT GCGTCAGCTT 
AGCTCACCAA CATCTCAGAA GCACGAGAAA GTGTGCGCTA GACTTTCCCT CACTTCTCCA 
GAGCAGTTCC CCTCTGTTCC TCTCAAAGAC CACTTCTTCT CCCAGAGCAC ACCTGTTGGA 
CTGGACTTTT TGGGCTGGCC TCACCGTGCA TCATTTTCAG AATCGAATCT CAGCACACCA 
GCACAAGAGG CACATGAACA GGACCCTCCT CTGGCCTTGG TGATAACTGA TGGACCGCAG 
GATAAGTCTG GCCTTGCGGA TGAGGTGGGG AGTGAAGATG ACCTGTACAA TGAGTTTCGC 
AGCTCCTCCA ACCGATTCGG ACACCCAGGA GGAGGAGGAG AACAGCTGGC CATAAATGAG 
CTGATCAGTG ATGGCAGCGT GTGCGCGGAG GCTCTATGGG ACCACGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGAGACGTC ATCGAAGTAG TGGACGCCAC AAACAAGGAG 
TGGTGGTGGG GCCGGGTGCT GGATAGCGAG GGCTGGTTCC CCGCCAGCTT TGTTCGGTTA 
CGGGTGAACC AAGATGAGCC CATGGAGGAA TACTTAGCCC ATCTGGACGG GGCCTCGGAG 
GGGGGTGGGG CCAGCATGGG GGACCCCTTA GGGCCCGGTC TGCCCTGCAA GGAGCAGATG 
AGAGCCAACG TCATCAATGA GATCATGAGC ACAGAGAGGG ATTACATCAA ACACCTGAAG 
GACATCTGTG AGGGCTATAT AAAACAGTGT CGGAAGAGGA CAGATATGTT TACTGAGGAG 
CAACTGCGCA CTATCTTTGG GAACATCGAT GAGCTCTACC GTTTCCAGAA GAAGTTTCTC 
AAAGCCTTGG AGAAGAAATT CAACAAAGAC CATCCTCACC TCAGCGAGAT TGGCTCCTGC 
TTCTTAGAGC ATCAAACAAA CTTTCAGATC TACTCTGAAT ATTGCAACAA CCACCCCAAT 
GCCTGCGTGC AGCTCTCCAA ACTGATGAAG ATCAAGAAGT ATGTGTTCTT CTTTGAGGCC 
TGTCGCCTGC TCCAGAAGAT GATCGACATT TCCTTGGATG GATTCCTGCT TACTCCTGTT 
CAGAAGATCT GCAAGTATCC TCTGCAGCTG GCTGAACTGC TGAAGTACAC CAACCCACAG 
CACAGAGATT ATAAGGATGT AGAAGCTGCC TTAAATGCAA TGAAGAATGT GGCCCGACTG 
ATCAATGAGA GAAAGAGGCG TCTGGAGAAT ATTGACAAAA TTGCTCAGTG GCAAAGCTCC 
ATAGAAGACT GGGAGGGTGA AGACATCTTG AGCAGAAGCT CTGATCTGAT TTTCTCAGGA 
GACCTGACCA AGATCTCCCA GCCACAGGGC AAAGGCCAGC AGCGAATGTT CTTTCTCTTT 
GACCACCAAC TGGTTTTCTG TAAGAAGGAT CTGCTTCGGA GGGACATCCT GTACTACAAG 
GGTCGGTTAG ACATGGATCA GATGCAAGTG GTGGATGTGG AGGATGGGAA GGATAAGGAC 
TTTAACGTGA GTGTGAAGAA TGCCTTGAAA TTATGTTCTC CTGTGGGAGA GGAGGTCCAC 
CTTCTGTGTG CCAAGAAACC CGAGCAGAAG CAGCGCTGGC TGCGAGCCTT TGCGGATGAA 
CGGGAACAGG TCCAGCACGA CCTCGAAACA GGTTTTACGA TCACAGAGGT CCAGAAGAAG 
CAGGCCATGT TGAATGCATC TAAAAGTCAT CCAACAGGAA AGCCCAAAGC ATTAGCGTTA 
CAGCATCTTT TGGCACTCAG AACTCCTTCA CTTCTTCACC CAAAAACTTC CAAGTGCCTA 
CGTTTTTCTG AACCTTCAGG ACCTCTGGCT CCCAGGGCCA CCTTAACTTC ACCTCTCCTC 
CCTGAAGGCC CTCAGCGTAG GCGGGGGCGG GGGGTTAGGG TGCCCCCTGG GGTCCTGCGC 
AGGGCAATAT CTTGGGTGTC CTCTCAAAGT AGAGAGCCGA TCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC107710620 cDNA ORF clone, Sinocyclocheilus rhinocerous

Gene

Clones

gRNAs

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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