LOC108934306 gene cDNA ORF clone, Scleropages formosus(Asian bonytongue)

mRNA	Protein	Name
XM_018751904.1	XP_018607420.1	collagen alpha-5(IV) chain-like isoform X4
XM_018751907.1	XP_018607423.1	collagen alpha-5(IV) chain-like isoform X7
XM_018751908.1	XP_018607424.1	collagen alpha-5(IV) chain-like isoform X8
XM_018751909.1	XP_018607425.1	collagen alpha-5(IV) chain-like isoform X9
XM_018751901.2	XP_018607417.2	collagen alpha-5(IV) chain-like isoform X1
XM_018751904.2	XP_018607420.2	collagen alpha-5(IV) chain-like isoform X5
XM_018751905.2	XP_018607421.2	collagen alpha-5(IV) chain-like isoform X6
XM_018751906.2	XP_018607422.2	collagen alpha-5(IV) chain-like isoform X7
XM_018751907.2	XP_018607423.2	collagen alpha-5(IV) chain-like isoform X8
XM_018751901.1	XP_018607417.1	collagen alpha-5(IV) chain-like isoform X1
XM_018751902.1	XP_018607418.1	collagen alpha-5(IV) chain-like isoform X2
XM_029250987.1	XP_029106820.1	collagen alpha-5(IV) chain-like isoform X2
XM_018751902.2	XP_018607418.2	collagen alpha-5(IV) chain-like isoform X3
XM_018751903.2	XP_018607419.2	collagen alpha-5(IV) chain-like isoform X4
XM_018751908.2	XP_018607424.2	collagen alpha-5(IV) chain-like isoform X9
XM_018751909.2	XP_018607425.2	collagen alpha-5(IV) chain-like isoform X10
XM_018751911.2	XP_018607427.2	collagen alpha-5(IV) chain-like isoform X11
XM_018751905.1	XP_018607421.1	collagen alpha-5(IV) chain-like isoform X5
XM_018751903.1	XP_018607419.1	collagen alpha-5(IV) chain-like isoform X3
XM_018751906.1	XP_018607422.1	collagen alpha-5(IV) chain-like isoform X6
XM_018751911.1	XP_018607427.1	collagen alpha-5(IV) chain-like isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc131351	XM_018751909.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSc131342	XM_018751901.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131343	XM_029250987.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131344	XM_018751902.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131345	XM_018751903.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131346	XM_018751904.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131347	XM_018751905.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131348	XM_018751906.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131349	XM_018751907.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131350	XM_018751908.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc131352	XM_018751911.2 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSc54529	XM_018751901.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54530	XM_018751902.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54531	XM_018751903.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54532	XM_018751904.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54533	XM_018751905.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54534	XM_018751906.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54535	XM_018751907.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54536	XM_018751908.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSc54537	XM_018751909.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OSc54538	XM_018751911.1 Latest version!	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

LOC108934306 ( XM_018751909.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607425.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OSc131351
Clone ID Related Accession (Same CDS sequence)	XM_018751909.2
Accession Version	XM_018751909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC GGAACACTCG 
GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC CCGGGCCAGA 
TTGCGGCCCA GATCGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607425.2
CDS	125..4345
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQEQEVCLARPEHSVTQETKEMSVHKDPRVTRARLRPRSW

Target ORF information:

RefSeq Version	XM_018751909.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751909.2

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC GGAACACTCG 
GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC CCGGGCCAGA 
TTGCGGCCCA GATCGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751901.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607417.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X1, mRNA.

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CloneID	OSc131342
Clone ID Related Accession (Same CDS sequence)	XM_018751901.2
Accession Version	XM_018751901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607417.2
CDS	125..5446
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751901.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751901.2

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_029250987.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029106820.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSc131343
Clone ID Related Accession (Same CDS sequence)	XM_029250987.1
Accession Version	XM_029250987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGAAAAT 
ACCTACCCGG TGGTCACCAT GGACAGTATG AAAGGGCAGG CGTTAGATCG GCAGGTCGTC 
TCGGCCCCGA GGGTCCACGC GGCCCCCCTG GCTCGGGAGG GCTCGAGGGG CAAAAGGGCG 
CTGCTGGTCC CCCTGGTCAG CCTGGCCTGC CTGGTCAGAA GGGAGAGAGC GGAACCGCAG 
GCCTTCCGGG ATCTCCCGGT CTTCCCGGGC CAGCGGGACT CAAAGGAGAC GCCGGCCTTC 
CCGGACAACC AGGGCTTCCA GGTCAGCGCG CGCCGCTTCG GTCTGCTCGT GCGCTCCGGT 
CTGTTCCTCA CACTAAGACG GAGACATAAA TCGGAGTTTC ACATGCGGGA CGCCGGAGGG 
ACCTTGAACA CTGTGCTCGT TGTGCTTTGC AGCCACAGCC GTGGCCTTTC GACGAAAGGA 
GCAGGAGGTC TGTCTGGCAC GCCCGGAACA CTCGGTGACC CAGGAGACAA AGGAGATGTC 
GGTCCACAAG GACCCCCGGG TGACCCGGGC CAGATTGCGG CCCAGATCGT GGTGAAAGGC 
GATCGGGGGC CCCCTGGTAC CCAGGGTCGC CCAGGTTCAC GTGGACCCGT GGGTCTTCCA 
GGTTTCGAGG GCTTGTCAGG TCCGCCAGGA GAACCTGGAG AACCGGGTGC TCGGGGTGCC 
ATAGGGTTCA GCGGACCTTT GGGTCACAAA GGAGACGACG GGCCACCTGG AGAGCCAGGT 
CAGAGGGGCT TCCCAGGCCC TCCCGGACCA GATGGGCTCC GGGGACCGTC GGGCACACCG 
GGGACCTCCT CGGCGGCTCA CGGCTTTCTG GTGACGCGCC ACAGCCAGTC TAGCGAGGAC 
CCCTTCTGCC CCGAGGGCAC GAGTCTCCTG TATCGTGGCT ACTCCCTGCT GTACGTGCAG 
GGCAACGAGC GCTCCCACGG TCAGGACTTG GGGACGGCGG GGAGCTGCCT GCGCAGGTTC 
AGCGCCATGC CGTTCATGTT CTGCAACATC AACAACGTCT GCAGCTTGGC GACGCGCAGC 
GACTACTCCT ACTGGCTGTC CACACCACAG GCCATGTCTG CGAGCATGGC GCCCGTCGCC 
GGGGACGACG TCAAGCCCTA CATCAGCAGG TGTGCGGTGT GTGAAGCTCC TGCGATGGTG 
GTCGCCGTGC ACAGCCAGTC CATCCGGCTG CCCTCCTGCC CGGCCGGCTG GGACCCGCTG 
TGGATCGGCT ACTCCTTCAT GATGCACACC GGTGCGGGGG CGGAGGGTTC CGGTCAAGCC 
CTGGCCTCCC CCGGCTCCTG CCTCGAGGAC TTCCGCTCCT CCCCCTTCGT CGAATGCCAC 
GGAAAAGGCG CCTGCAATTA CTACGCCGAC TCCTACAGCT TCTGGCTGGC CACCGTGGAC 
GACGGCGACA TGTTTCGGAA GCCTCAGGAG GAGACCCTGA AAGCAGGAGA CCTTGAGACG 
CGGGTGAGCC GCTGTGCGGT GTGCATGAAG AGGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029106820.1
CDS	125..5443
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_029250987.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029250987.1

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGAAAAT 
ACCTACCCGG TGGTCACCAT GGACAGTATG AAAGGGCAGG CGTTAGATCG GCAGGTCGTC 
TCGGCCCCGA GGGTCCACGC GGCCCCCCTG GCTCGGGAGG GCTCGAGGGG CAAAAGGGCG 
CTGCTGGTCC CCCTGGTCAG CCTGGCCTGC CTGGTCAGAA GGGAGAGAGC GGAACCGCAG 
GCCTTCCGGG ATCTCCCGGT CTTCCCGGGC CAGCGGGACT CAAAGGAGAC GCCGGCCTTC 
CCGGACAACC AGGGCTTCCA GGTCAGCGCG CGCCGCTTCG GTCTGCTCGT GCGCTCCGGT 
CTGTTCCTCA CACTAAGACG GAGACATAAA TCGGAGTTTC ACATGCGGGA CGCCGGAGGG 
ACCTTGAACA CTGTGCTCGT TGTGCTTTGC AGCCACAGCC GTGGCCTTTC GACGAAAGGA 
GCAGGAGGTC TGTCTGGCAC GCCCGGAACA CTCGGTGACC CAGGAGACAA AGGAGATGTC 
GGTCCACAAG GACCCCCGGG TGACCCGGGC CAGATTGCGG CCCAGATCGT GGTGAAAGGC 
GATCGGGGGC CCCCTGGTAC CCAGGGTCGC CCAGGTTCAC GTGGACCCGT GGGTCTTCCA 
GGTTTCGAGG GCTTGTCAGG TCCGCCAGGA GAACCTGGAG AACCGGGTGC TCGGGGTGCC 
ATAGGGTTCA GCGGACCTTT GGGTCACAAA GGAGACGACG GGCCACCTGG AGAGCCAGGT 
CAGAGGGGCT TCCCAGGCCC TCCCGGACCA GATGGGCTCC GGGGACCGTC GGGCACACCG 
GGGACCTCCT CGGCGGCTCA CGGCTTTCTG GTGACGCGCC ACAGCCAGTC TAGCGAGGAC 
CCCTTCTGCC CCGAGGGCAC GAGTCTCCTG TATCGTGGCT ACTCCCTGCT GTACGTGCAG 
GGCAACGAGC GCTCCCACGG TCAGGACTTG GGGACGGCGG GGAGCTGCCT GCGCAGGTTC 
AGCGCCATGC CGTTCATGTT CTGCAACATC AACAACGTCT GCAGCTTGGC GACGCGCAGC 
GACTACTCCT ACTGGCTGTC CACACCACAG GCCATGTCTG CGAGCATGGC GCCCGTCGCC 
GGGGACGACG TCAAGCCCTA CATCAGCAGG TGTGCGGTGT GTGAAGCTCC TGCGATGGTG 
GTCGCCGTGC ACAGCCAGTC CATCCGGCTG CCCTCCTGCC CGGCCGGCTG GGACCCGCTG 
TGGATCGGCT ACTCCTTCAT GATGCACACC GGTGCGGGGG CGGAGGGTTC CGGTCAAGCC 
CTGGCCTCCC CCGGCTCCTG CCTCGAGGAC TTCCGCTCCT CCCCCTTCGT CGAATGCCAC 
GGAAAAGGCG CCTGCAATTA CTACGCCGAC TCCTACAGCT TCTGGCTGGC CACCGTGGAC 
GACGGCGACA TGTTTCGGAA GCCTCAGGAG GAGACCCTGA AAGCAGGAGA CCTTGAGACG 
CGGGTGAGCC GCTGTGCGGT GTGCATGAAG AGGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751902.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607418.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X3, mRNA.

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CloneID	OSc131344
Clone ID Related Accession (Same CDS sequence)	XM_018751902.2
Accession Version	XM_018751902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGCG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607418.2
CDS	150..5423
Translation	MKTSCGTRVRNLLRVGLLLSASLGVRALQGNMCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751902.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751902.2

ORF Insert Sequence:

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGCG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751903.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607419.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X4, mRNA.

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CloneID	OSc131345
Clone ID Related Accession (Same CDS sequence)	XM_018751903.2
Accession Version	XM_018751903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607419.2
CDS	125..5386
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQRSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751903.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751903.2

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751904.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607420.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X5, mRNA.

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CloneID	OSc131346
Clone ID Related Accession (Same CDS sequence)	XM_018751904.2
Accession Version	XM_018751904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGCG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607420.2
CDS	150..5363
Translation	MKTSCGTRVRNLLRVGLLLSASLGVRALQGNGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751904.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751904.2

ORF Insert Sequence:

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGCG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751905.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607421.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X6, mRNA.

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CloneID	OSc131347
Clone ID Related Accession (Same CDS sequence)	XM_018751905.2
Accession Version	XM_018751905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT TTGGCTGCAC GGGAGACTCC GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC 
AAGGGTCAAC GGGGCCTTCC TGGACTTCAG GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG 
CCGGAGGGGC AGGAGGGACC CAGAGGGGAG AAGGGCGCCG ACGGGCCGCC GGGACCGGCC 
GGTCCGAAAG GAATCCGGGG TCCTCCCGGA CAGCCTGGAT TTCCGGGAAC CCCAGGAATC 
CCGGGTCTAC CAGGACAGGA TGGCCCCCGG GGACCCACGG GACCGGCGGG GTGCAATGGG 
ACCAAGGGTG AACGAGGATT GCCTGGAAAC TCTGGCTTTC CTGGGGTACA AGGTCCCCCG 
GGTCCATCTG GTATACCGGG CGCAAAGGGA GACCCTGGTT TGATCCTCTC GGCGGGTCCA 
TTCGGACAGA TGGGAGAACC GGGCTCGCCT GGACTTCCAG GTGTTCCTGG CAGTCAAGGC 
CTTCCGGGAC TAGCAGGCCC AACAGGACCA CCCGGACCCC CAGGCTTTCA GGGGAGGCCT 
GGACCACCGG GGCCTCCAGG GCCCAAGGGT AACATGGGCC TGAACTTCCA AGGAGAGAAG 
GGGAAGAAGG GTGAACAAGG AATGCGTGGG CAACCGGGGC CCCCCGGCCT CATTCGTGAG 
CAGAAGCAAG CGAGGGATTC GGAGGTGCGG AGGGGAAACA AGGGGGATCA GGGACCACCT 
GGTCCACCTG GGAAACCTGG GGTTCCTGGG CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT 
GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG AGGGGGAAGC CAGGGAAAGA TGGTGATCCT 
GGACCAGTCG GTTTCCCGGG TTCGAATGGA GAGCCTGGGG GTCCGGGTTT GCCCGGAACG 
CATGGTATTA CGGGCGAGAA AGGAGACCGC GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG 
ACAGGTTCGA ATCGTATCAG TGAAGGACCA AAGGGAGAGC CTGGTTTACC TGGACAGCTT 
GGGCAGAAAG GAGAGTCAGG TCCACCAGGT CTTCCGGGAC TTCCTGGACA GCCAGGTGCT 
CCAGGGTTTG GCTCCCCAGG CTCACCTGGT CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG 
AGAGGTAATG AGGGACAGCC AGGAGTATCT TTGCCTGGAC TGCCGGGGAG AGACGGGCAG 
CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA GGGCTGCCAG GAACATCATC TGGAAATGGG 
CTCTGCCTTC GGGGGACGCC AGGGCTTCCA GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA 
GACAGAGGGC AGCCGGGTGA CAAAGGGGAG ACCTGCATTA ACTGCTTCTC CGGTGCCGAT 
GCCATTCCGG GGCCTCCGGG TCCTCCCGGT CCATCCGGAT TCACAGGTCC TCCAGGACCA 
CCTGGGATTA AAGGTGACAA AGGGATCCCA GGAATCCCTG GACAATCTGG CCCTCCAGGA 
TTGCCTGGGG ACCAAGGACC GCCAGGTGTT GCAGGACAGA AAGGAGACCC GGGAGATGCT 
GTCTCTATTG GTAGGATGAA GGGCGATAAA GGAGACACGG GCTTTCCGGG TCCACCTGGT 
CTACCTGGCC TGAATGGAAG ACCTGGCCAA GACGGAATCC CAGGTTCACC TGGACCAATA 
GGGTTACCTG GATCTCTCTT CGTTAAAGAG GAACATGGCC CACCAGGGGA ACCTGGAATC 
CCAGGCATTC CTGGGGACAG GGGATCTCCA GGAGCACCAG GGTTTGGCCT TCAAGGGCCT 
CCAGGAGAGA AAGGTGTCCA AGGTGTATCG GGGCGGGCAG GTCCACCTGG TCCCCCAGGT 
CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA GCGAAGGGTG TCCCTGGTCC TATAGGGCTG 
AGTGGCGAGC CTGGCATCGC AGGACCTCCT GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG 
TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC CTTGGACTTC CTGGCATTGG CTTACCGGGT 
CCTCCAGGAG TCAAAGGATT CCCTGGGACA CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA 
GGACCACCTG GATTAGATGG CCTCCCTGGG CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC 
CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA CCTCCTGGAT TTCCTGGTAG CAAAGGAACG 
CAAGGCCCTA AAGGAGACCC TGGTTTCCCA GGGATCCCTG GCCAGCCTGG GCGATCAGGA 
GTTGATGGTG CTCCAGGACC CAAAGGGGAT CCAGGCATTC CTGGGACCCC TGGAGCTCCT 
GGGCCCCCTG GTGCTACTTC CTTTGGGCTT CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA 
GGTGCTGTAG GATCTCCAGG GTTCCCTGGA GCGAATGGTG ACAAAGGGGA CCCTGGACCC 
CCGAGGCCGA ACATCCCAGG ACTCCCAGGG GTTAAAGGGG AACCGGGCTC CCCTGGACTC 
CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT GGTCAAACGG GTCGAGAGGG ACTTCCTGGA 
TTTCCTGGCC CCAAAGGAGA GATGGGGGTG ATGGGCACCG CAGGCCCTCT TGGACCTCCA 
GGAAACTCTG GTGTTCCTGG ACTCACAGGA CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA 
CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG GGGGATCGAG GGGATCCTGG TATTCCTGGT 
CCCCCTGGCC CAAGACTTGT CGTGCAGCAG CTACAGGGAG CAAAGGGAGA AGAGGGTCTT 
CCAGGTGCAG CCGGAGTGTC GGGACACAAG GGTGCGCAAG GACTCCCAGG AGATGCTGGT 
CTTCCTGAAC TTGACGGAAT ACCAGGACAG CCGGGACCTC CAGGACCCAA AGGAGAGCCT 
GGGCTCCCGG GTGAACCAGG GTCCCCAGGA CCACCGGGAC CAAAAGGCAA TATGGGCGAG 
ATGGGATTCC CAGGTCCCAG TGGGGTGAAA GGTAACCCTG GACAGCCAGG AAGGTCGGGC 
CCACTTGGAC AGCCAGGCAC CCCTGGTTTC AGGGGAGAGA AGGGTGATCC TGGTCCTCCT 
GGGTTTGGAG TCCCAGGAAA TCCAGGTACC AAGGGTGAAC TGGGACAACC TGGATTCCCT 
GGAATCCCAG GACCCAAAGG CCAGTCAGGT GCACCGGGTT TACCGGGTCT GCCTGGCAGT 
CCAGGAGAAA AGAGAAACGC AGGACTCCCT GGAATTCCAG GCCTTCCGGG TATTCCTGGA 
CCAAAAGGCT TCGATGGAGC TCCAGGATCC CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA 
GGAGAACCAG GCCGTCCTGG ACCGCCTGGG CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC 
GATGGCATAC CTGGACCGTC TGGGGTGAAA GGAGACCCGG GTCAGCCTGG CTCGGGTCGC 
CTTGGTGTTC CAGGTCCCAC AGGGTTACCA GGACCAAAGG GAGGCGGTGG CGTCCCTGGT 
CTTCCTGGAA GCCCCGGTTT CCCTGGACGA AAGGGTGAGA CGGGAACGCC CGGCATACCC 
GGATACTCCG GAAACGTCGG ACCTCCGGGT CCCCCCGGTC CGTCACGAGC GGGGCCCAAA 
GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA GGAAGCGCAG AAAATACCTA CCCGGTGGTC 
ACCATGGACA GTATGAAAGG GCAGGCGTTA GATCGGCAGG TCGTCTCGGC CCCGAGGGTC 
CACGCGGCCC CCCTGGCTCG GGAGGGCTCG AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG 
GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG AGAGCGGAAC CGCAGGCCTT CCGGGATCTC 
CCGGTCTTCC CGGGCCAGCG GGACTCAAAG GAGACGCCGG CCTTCCCGGA CAACCAGGGC 
TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG CTCGTGCGCT CCGGTCTGTT CCTCACACTA 
AGACGGAGAC ATAAATCGGA GTTTCACATG CGGGACGCCG GAGGGACCTT GAACACTGTG 
CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC CTTTCGACGA AAGGAGCAGG AGGTCTGTCT 
GGCACGCCCG GAACACTCGG TGACCCAGGA GACAAAGGAG ATGTCGGTCC ACAAGGACCC 
CCGGGTGACC CGGGCCAGAT TGCGGCCCAG ATCGTGGTGA AAGGCGATCG GGGGCCCCCT 
GGTACCCAGG GTCGCCCAGG TTCACGTGGA CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG 
TCAGGTCCGC CAGGAGAACC TGGAGAACCG GGTGCTCGGG GTGCCATAGG GTTCAGCGGA 
CCTTTGGGTC ACAAAGGAGA CGACGGGCCA CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA 
GGCCCTCCCG GACCAGATGG GCTCCGGGGA CCGTCGGGCA CACCGGGGAC CTCCTCGGCG 
GCTCACGGCT TTCTGGTGAC GCGCCACAGC CAGTCTAGCG AGGACCCCTT CTGCCCCGAG 
GGCACGAGTC TCCTGTATCG TGGCTACTCC CTGCTGTACG TGCAGGGCAA CGAGCGCTCC 
CACGGTCAGG ACTTGGGGAC GGCGGGGAGC TGCCTGCGCA GGTTCAGCGC CATGCCGTTC 
ATGTTCTGCA ACATCAACAA CGTCTGCAGC TTGGCGACGC GCAGCGACTA CTCCTACTGG 
CTGTCCACAC CACAGGCCAT GTCTGCGAGC ATGGCGCCCG TCGCCGGGGA CGACGTCAAG 
CCCTACATCA GCAGGTGTGC GGTGTGTGAA GCTCCTGCGA TGGTGGTCGC CGTGCACAGC 
CAGTCCATCC GGCTGCCCTC CTGCCCGGCC GGCTGGGACC CGCTGTGGAT CGGCTACTCC 
TTCATGATGC ACACCGGTGC GGGGGCGGAG GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC 
TCCTGCCTCG AGGACTTCCG CTCCTCCCCC TTCGTCGAAT GCCACGGAAA AGGCGCCTGC 
AATTACTACG CCGACTCCTA CAGCTTCTGG CTGGCCACCG TGGACGACGG CGACATGTTT 
CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA GGAGACCTTG AGACGCGGGT GAGCCGCTGT 
GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607421.2
CDS	34..5217
Translation	MMCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751905.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751905.2

ORF Insert Sequence:

ATGATGTGTT TTGGCTGCAC GGGAGACTCC GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC 
AAGGGTCAAC GGGGCCTTCC TGGACTTCAG GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG 
CCGGAGGGGC AGGAGGGACC CAGAGGGGAG AAGGGCGCCG ACGGGCCGCC GGGACCGGCC 
GGTCCGAAAG GAATCCGGGG TCCTCCCGGA CAGCCTGGAT TTCCGGGAAC CCCAGGAATC 
CCGGGTCTAC CAGGACAGGA TGGCCCCCGG GGACCCACGG GACCGGCGGG GTGCAATGGG 
ACCAAGGGTG AACGAGGATT GCCTGGAAAC TCTGGCTTTC CTGGGGTACA AGGTCCCCCG 
GGTCCATCTG GTATACCGGG CGCAAAGGGA GACCCTGGTT TGATCCTCTC GGCGGGTCCA 
TTCGGACAGA TGGGAGAACC GGGCTCGCCT GGACTTCCAG GTGTTCCTGG CAGTCAAGGC 
CTTCCGGGAC TAGCAGGCCC AACAGGACCA CCCGGACCCC CAGGCTTTCA GGGGAGGCCT 
GGACCACCGG GGCCTCCAGG GCCCAAGGGT AACATGGGCC TGAACTTCCA AGGAGAGAAG 
GGGAAGAAGG GTGAACAAGG AATGCGTGGG CAACCGGGGC CCCCCGGCCT CATTCGTGAG 
CAGAAGCAAG CGAGGGATTC GGAGGTGCGG AGGGGAAACA AGGGGGATCA GGGACCACCT 
GGTCCACCTG GGAAACCTGG GGTTCCTGGG CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT 
GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG AGGGGGAAGC CAGGGAAAGA TGGTGATCCT 
GGACCAGTCG GTTTCCCGGG TTCGAATGGA GAGCCTGGGG GTCCGGGTTT GCCCGGAACG 
CATGGTATTA CGGGCGAGAA AGGAGACCGC GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG 
ACAGGTTCGA ATCGTATCAG TGAAGGACCA AAGGGAGAGC CTGGTTTACC TGGACAGCTT 
GGGCAGAAAG GAGAGTCAGG TCCACCAGGT CTTCCGGGAC TTCCTGGACA GCCAGGTGCT 
CCAGGGTTTG GCTCCCCAGG CTCACCTGGT CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG 
AGAGGTAATG AGGGACAGCC AGGAGTATCT TTGCCTGGAC TGCCGGGGAG AGACGGGCAG 
CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA GGGCTGCCAG GAACATCATC TGGAAATGGG 
CTCTGCCTTC GGGGGACGCC AGGGCTTCCA GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA 
GACAGAGGGC AGCCGGGTGA CAAAGGGGAG ACCTGCATTA ACTGCTTCTC CGGTGCCGAT 
GCCATTCCGG GGCCTCCGGG TCCTCCCGGT CCATCCGGAT TCACAGGTCC TCCAGGACCA 
CCTGGGATTA AAGGTGACAA AGGGATCCCA GGAATCCCTG GACAATCTGG CCCTCCAGGA 
TTGCCTGGGG ACCAAGGACC GCCAGGTGTT GCAGGACAGA AAGGAGACCC GGGAGATGCT 
GTCTCTATTG GTAGGATGAA GGGCGATAAA GGAGACACGG GCTTTCCGGG TCCACCTGGT 
CTACCTGGCC TGAATGGAAG ACCTGGCCAA GACGGAATCC CAGGTTCACC TGGACCAATA 
GGGTTACCTG GATCTCTCTT CGTTAAAGAG GAACATGGCC CACCAGGGGA ACCTGGAATC 
CCAGGCATTC CTGGGGACAG GGGATCTCCA GGAGCACCAG GGTTTGGCCT TCAAGGGCCT 
CCAGGAGAGA AAGGTGTCCA AGGTGTATCG GGGCGGGCAG GTCCACCTGG TCCCCCAGGT 
CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA GCGAAGGGTG TCCCTGGTCC TATAGGGCTG 
AGTGGCGAGC CTGGCATCGC AGGACCTCCT GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG 
TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC CTTGGACTTC CTGGCATTGG CTTACCGGGT 
CCTCCAGGAG TCAAAGGATT CCCTGGGACA CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA 
GGACCACCTG GATTAGATGG CCTCCCTGGG CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC 
CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA CCTCCTGGAT TTCCTGGTAG CAAAGGAACG 
CAAGGCCCTA AAGGAGACCC TGGTTTCCCA GGGATCCCTG GCCAGCCTGG GCGATCAGGA 
GTTGATGGTG CTCCAGGACC CAAAGGGGAT CCAGGCATTC CTGGGACCCC TGGAGCTCCT 
GGGCCCCCTG GTGCTACTTC CTTTGGGCTT CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA 
GGTGCTGTAG GATCTCCAGG GTTCCCTGGA GCGAATGGTG ACAAAGGGGA CCCTGGACCC 
CCGAGGCCGA ACATCCCAGG ACTCCCAGGG GTTAAAGGGG AACCGGGCTC CCCTGGACTC 
CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT GGTCAAACGG GTCGAGAGGG ACTTCCTGGA 
TTTCCTGGCC CCAAAGGAGA GATGGGGGTG ATGGGCACCG CAGGCCCTCT TGGACCTCCA 
GGAAACTCTG GTGTTCCTGG ACTCACAGGA CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA 
CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG GGGGATCGAG GGGATCCTGG TATTCCTGGT 
CCCCCTGGCC CAAGACTTGT CGTGCAGCAG CTACAGGGAG CAAAGGGAGA AGAGGGTCTT 
CCAGGTGCAG CCGGAGTGTC GGGACACAAG GGTGCGCAAG GACTCCCAGG AGATGCTGGT 
CTTCCTGAAC TTGACGGAAT ACCAGGACAG CCGGGACCTC CAGGACCCAA AGGAGAGCCT 
GGGCTCCCGG GTGAACCAGG GTCCCCAGGA CCACCGGGAC CAAAAGGCAA TATGGGCGAG 
ATGGGATTCC CAGGTCCCAG TGGGGTGAAA GGTAACCCTG GACAGCCAGG AAGGTCGGGC 
CCACTTGGAC AGCCAGGCAC CCCTGGTTTC AGGGGAGAGA AGGGTGATCC TGGTCCTCCT 
GGGTTTGGAG TCCCAGGAAA TCCAGGTACC AAGGGTGAAC TGGGACAACC TGGATTCCCT 
GGAATCCCAG GACCCAAAGG CCAGTCAGGT GCACCGGGTT TACCGGGTCT GCCTGGCAGT 
CCAGGAGAAA AGAGAAACGC AGGACTCCCT GGAATTCCAG GCCTTCCGGG TATTCCTGGA 
CCAAAAGGCT TCGATGGAGC TCCAGGATCC CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA 
GGAGAACCAG GCCGTCCTGG ACCGCCTGGG CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC 
GATGGCATAC CTGGACCGTC TGGGGTGAAA GGAGACCCGG GTCAGCCTGG CTCGGGTCGC 
CTTGGTGTTC CAGGTCCCAC AGGGTTACCA GGACCAAAGG GAGGCGGTGG CGTCCCTGGT 
CTTCCTGGAA GCCCCGGTTT CCCTGGACGA AAGGGTGAGA CGGGAACGCC CGGCATACCC 
GGATACTCCG GAAACGTCGG ACCTCCGGGT CCCCCCGGTC CGTCACGAGC GGGGCCCAAA 
GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA GGAAGCGCAG AAAATACCTA CCCGGTGGTC 
ACCATGGACA GTATGAAAGG GCAGGCGTTA GATCGGCAGG TCGTCTCGGC CCCGAGGGTC 
CACGCGGCCC CCCTGGCTCG GGAGGGCTCG AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG 
GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG AGAGCGGAAC CGCAGGCCTT CCGGGATCTC 
CCGGTCTTCC CGGGCCAGCG GGACTCAAAG GAGACGCCGG CCTTCCCGGA CAACCAGGGC 
TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG CTCGTGCGCT CCGGTCTGTT CCTCACACTA 
AGACGGAGAC ATAAATCGGA GTTTCACATG CGGGACGCCG GAGGGACCTT GAACACTGTG 
CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC CTTTCGACGA AAGGAGCAGG AGGTCTGTCT 
GGCACGCCCG GAACACTCGG TGACCCAGGA GACAAAGGAG ATGTCGGTCC ACAAGGACCC 
CCGGGTGACC CGGGCCAGAT TGCGGCCCAG ATCGTGGTGA AAGGCGATCG GGGGCCCCCT 
GGTACCCAGG GTCGCCCAGG TTCACGTGGA CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG 
TCAGGTCCGC CAGGAGAACC TGGAGAACCG GGTGCTCGGG GTGCCATAGG GTTCAGCGGA 
CCTTTGGGTC ACAAAGGAGA CGACGGGCCA CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA 
GGCCCTCCCG GACCAGATGG GCTCCGGGGA CCGTCGGGCA CACCGGGGAC CTCCTCGGCG 
GCTCACGGCT TTCTGGTGAC GCGCCACAGC CAGTCTAGCG AGGACCCCTT CTGCCCCGAG 
GGCACGAGTC TCCTGTATCG TGGCTACTCC CTGCTGTACG TGCAGGGCAA CGAGCGCTCC 
CACGGTCAGG ACTTGGGGAC GGCGGGGAGC TGCCTGCGCA GGTTCAGCGC CATGCCGTTC 
ATGTTCTGCA ACATCAACAA CGTCTGCAGC TTGGCGACGC GCAGCGACTA CTCCTACTGG 
CTGTCCACAC CACAGGCCAT GTCTGCGAGC ATGGCGCCCG TCGCCGGGGA CGACGTCAAG 
CCCTACATCA GCAGGTGTGC GGTGTGTGAA GCTCCTGCGA TGGTGGTCGC CGTGCACAGC 
CAGTCCATCC GGCTGCCCTC CTGCCCGGCC GGCTGGGACC CGCTGTGGAT CGGCTACTCC 
TTCATGATGC ACACCGGTGC GGGGGCGGAG GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC 
TCCTGCCTCG AGGACTTCCG CTCCTCCCCC TTCGTCGAAT GCCACGGAAA AGGCGCCTGC 
AATTACTACG CCGACTCCTA CAGCTTCTGG CTGGCCACCG TGGACGACGG CGACATGTTT 
CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA GGAGACCTTG AGACGCGGGT GAGCCGCTGT 
GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751906.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607422.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X7, mRNA.

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CloneID	OSc131348
Clone ID Related Accession (Same CDS sequence)	XM_018751906.2
Accession Version	XM_018751906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTTG GCTGCACGGG AGACTCCGCT TGTGCCTGCA GTGGCGTGAA AGGAGACAAG 
GGTCAACGGG GCCTTCCTGG ACTTCAGGGT CCGCCGGGGC TTCCGGGATT TCCTGGGCCG 
GAGGGGCAGG AGGGACCCAG AGGGGAGAAG GGCGCCGACG GGCCGCCGGG ACCGGCCGGT 
CCGAAAGGAA TCCGGGGTCC TCCCGGACAG CCTGGATTTC CGGGAACCCC AGGAATCCCG 
GGTCTACCAG GACAGGATGG CCCCCGGGGA CCCACGGGAC CGGCGGGGTG CAATGGGACC 
AAGGGTGAAC GAGGATTGCC TGGAAACTCT GGCTTTCCTG GGGTACAAGG TCCCCCGGGT 
CCATCTGGTA TACCGGGCGC AAAGGGAGAC CCTGGTTTGA TCCTCTCGGC GGGTCCATTC 
GGACAGATGG GAGAACCGGG CTCGCCTGGA CTTCCAGGTG TTCCTGGCAG TCAAGGCCTT 
CCGGGACTAG CAGGCCCAAC AGGACCACCC GGACCCCCAG GCTTTCAGGG GAGGCCTGGA 
CCACCGGGGC CTCCAGGGCC CAAGGGTAAC ATGGGCCTGA ACTTCCAAGG AGAGAAGGGG 
AAGAAGGGTG AACAAGGAAT GCGTGGGCAA CCGGGGCCCC CCGGCCTCAT TCGTGAGCAG 
AAGCAAGCGA GGGATTCGGA GGTGCGGAGG GGAAACAAGG GGGATCAGGG ACCACCTGGT 
CCACCTGGGA AACCTGGGGT TCCTGGGCCT CTCGGTCCTC CCGGTGGTCC GAAGGGTGAG 
AAGGGGGAGC CTGGGGAACC AGGCAAGAGG GGGAAGCCAG GGAAAGATGG TGATCCTGGA 
CCAGTCGGTT TCCCGGGTTC GAATGGAGAG CCTGGGGGTC CGGGTTTGCC CGGAACGCAT 
GGTATTACGG GCGAGAAAGG AGACCGCGGT CTCCCAGGCC TCCCGGGTGC AGTGGTGACA 
GGTTCGAATC GTATCAGTGA AGGACCAAAG GGAGAGCCTG GTTTACCTGG ACAGCTTGGG 
CAGAAAGGAG AGTCAGGTCC ACCAGGTCTT CCGGGACTTC CTGGACAGCC AGGTGCTCCA 
GGGTTTGGCT CCCCAGGCTC ACCTGGTCAG CCCGGTTTTC CTGGGGACAG AGGGCAGAGA 
GGTAATGAGG GACAGCCAGG AGTATCTTTG CCTGGACTGC CGGGGAGAGA CGGGCAGCCT 
GGGCCTCCGG GCCCCCCGGG GCCCCCAGGG CTGCCAGGAA CATCATCTGG AAATGGGCTC 
TGCCTTCGGG GGACGCCAGG GCTTCCAGGC CTTCGGGGAG AGCAAGGATT TCCAGGAGAC 
AGAGGGCAGC CGGGTGACAA AGGGGAGACC TGCATTAACT GCTTCTCCGG TGCCGATGCC 
ATTCCGGGGC CTCCGGGTCC TCCCGGTCCA TCCGGATTCA CAGGTCCTCC AGGACCACCT 
GGGATTAAAG GTGACAAAGG GATCCCAGGA ATCCCTGGAC AATCTGGCCC TCCAGGATTG 
CCTGGGGACC AAGGACCGCC AGGTGTTGCA GGACAGAAAG GAGACCCGGG AGATGCTGTC 
TCTATTGGTA GGATGAAGGG CGATAAAGGA GACACGGGCT TTCCGGGTCC ACCTGGTCTA 
CCTGGCCTGA ATGGAAGACC TGGCCAAGAC GGAATCCCAG GTTCACCTGG ACCAATAGGG 
TTACCTGGAT CTCTCTTCGT TAAAGAGGAA CATGGCCCAC CAGGGGAACC TGGAATCCCA 
GGCATTCCTG GGGACAGGGG ATCTCCAGGA GCACCAGGGT TTGGCCTTCA AGGGCCTCCA 
GGAGAGAAAG GTGTCCAAGG TGTATCGGGG CGGGCAGGTC CACCTGGTCC CCCAGGTCAA 
AAAGGTGAGC CAGGCCAGGT TGTTTCAGCG AAGGGTGTCC CTGGTCCTAT AGGGCTGAGT 
GGCGAGCCTG GCATCGCAGG ACCTCCTGGT GAGTCCGGCC AGCCTGGACA GCCTGGGTTC 
CCAGGGTTGC CTGGAGCAAA GGGAGACCTT GGACTTCCTG GCATTGGCTT ACCGGGTCCT 
CCAGGAGTCA AAGGATTCCC TGGGACACCA GGTCAGCCTG GAAGCTCTGG CAGCCCAGGA 
CCACCTGGAT TAGATGGCCT CCCTGGGCTG CCAGGCCCCC CAGGGGCCAA GGGTGACCCT 
GGGTTTGGGC TGCCAGGACC TGAAGGACCT CCTGGATTTC CTGGTAGCAA AGGAACGCAA 
GGCCCTAAAG GAGACCCTGG TTTCCCAGGG ATCCCTGGCC AGCCTGGGCG ATCAGGAGTT 
GATGGTGCTC CAGGACCCAA AGGGGATCCA GGCATTCCTG GGACCCCTGG AGCTCCTGGG 
CCCCCTGGTG CTACTTCCTT TGGGCTTCAG GGTCCTCCTG GCCCTCCAGG TCCCGCAGGT 
GCTGTAGGAT CTCCAGGGTT CCCTGGAGCG AATGGTGACA AAGGGGACCC TGGACCCCCG 
AGGCCGAACA TCCCAGGACT CCCAGGGGTT AAAGGGGAAC CGGGCTCCCC TGGACTCCCA 
GGCTCTATGG GTCCTCCTGG TTCTTCTGGT CAAACGGGTC GAGAGGGACT TCCTGGATTT 
CCTGGCCCCA AAGGAGAGAT GGGGGTGATG GGCACCGCAG GCCCTCTTGG ACCTCCAGGA 
AACTCTGGTG TTCCTGGACT CACAGGACCA AAAGGTGAGG GTGGCTCTCC CGGGCAACCT 
GGTGGACCAG GTGCTCCTGG GCCAAAGGGG GATCGAGGGG ATCCTGGTAT TCCTGGTCCC 
CCTGGCCCAA GACTTGTCGT GCAGCAGCTA CAGGGAGCAA AGGGAGAAGA GGGTCTTCCA 
GGTGCAGCCG GAGTGTCGGG ACACAAGGGT GCGCAAGGAC TCCCAGGAGA TGCTGGTCTT 
CCTGAACTTG ACGGAATACC AGGACAGCCG GGACCTCCAG GACCCAAAGG AGAGCCTGGG 
CTCCCGGGTG AACCAGGGTC CCCAGGACCA CCGGGACCAA AAGGCAATAT GGGCGAGATG 
GGATTCCCAG GTCCCAGTGG GGTGAAAGGT AACCCTGGAC AGCCAGGAAG GTCGGGCCCA 
CTTGGACAGC CAGGCACCCC TGGTTTCAGG GGAGAGAAGG GTGATCCTGG TCCTCCTGGG 
TTTGGAGTCC CAGGAAATCC AGGTACCAAG GGTGAACTGG GACAACCTGG ATTCCCTGGA 
ATCCCAGGAC CCAAAGGCCA GTCAGGTGCA CCGGGTTTAC CGGGTCTGCC TGGCAGTCCA 
GGAGAAAAGA GAAACGCAGG ACTCCCTGGA ATTCCAGGCC TTCCGGGTAT TCCTGGACCA 
AAAGGCTTCG ATGGAGCTCC AGGATCCCCA GGCGCTGATG GGCCAACAGG TCAGCCAGGA 
GAACCAGGCC GTCCTGGACC GCCTGGGCTG CCAGGAGACA AAGGCCCGCT TGGGCGCGAT 
GGCATACCTG GACCGTCTGG GGTGAAAGGA GACCCGGGTC AGCCTGGCTC GGGTCGCCTT 
GGTGTTCCAG GTCCCACAGG GTTACCAGGA CCAAAGGGAG GCGGTGGCGT CCCTGGTCTT 
CCTGGAAGCC CCGGTTTCCC TGGACGAAAG GGTGAGACGG GAACGCCCGG CATACCCGGA 
TACTCCGGAA ACGTCGGACC TCCGGGTCCC CCCGGTCCGT CACGAGCGGG GCCCAAAGGT 
AGCGCAGGGC CTCCGGGTGC CCCAGGAGGA AGCGCAGAAA ATACCTACCC GGTGGTCACC 
ATGGACAGTA TGAAAGGGCA GGCGTTAGAT CGGCAGGTCG TCTCGGCCCC GAGGGTCCAC 
GCGGCCCCCC TGGCTCGGGA GGGCTCGAGG GGCAAAAGGG CGCTGCTGGT CCCCCTGGTC 
AGCCTGGCCT GCCTGGTCAG AAGGGAGAGA GCGGAACCGC AGGCCTTCCG GGATCTCCCG 
GTCTTCCCGG GCCAGCGGGA CTCAAAGGAG ACGCCGGCCT TCCCGGACAA CCAGGGCTTC 
CAGGTCAGCG CGCGCCGCTT CGGTCTGCTC GTGCGCTCCG GTCTGTTCCT CACACTAAGA 
CGGAGACATA AATCGGAGTT TCACATGCGG GACGCCGGAG GGACCTTGAA CACTGTGCTC 
GTTGTGCTTT GCAGCCACAG CCGTGGCCTT TCGACGAAAG GAGCAGGAGG TCTGTCTGGC 
ACGCCCGGAA CACTCGGTGA CCCAGGAGAC AAAGGAGATG TCGGTCCACA AGGACCCCCG 
GGTGACCCGG GCCAGATTGC GGCCCAGATC GTGGTGAAAG GCGATCGGGG GCCCCCTGGT 
ACCCAGGGTC GCCCAGGTTC ACGTGGACCC GTGGGTCTTC CAGGTTTCGA GGGCTTGTCA 
GGTCCGCCAG GAGAACCTGG AGAACCGGGT GCTCGGGGTG CCATAGGGTT CAGCGGACCT 
TTGGGTCACA AAGGAGACGA CGGGCCACCT GGAGAGCCAG GTCAGAGGGG CTTCCCAGGC 
CCTCCCGGAC CAGATGGGCT CCGGGGACCG TCGGGCACAC CGGGGACCTC CTCGGCGGCT 
CACGGCTTTC TGGTGACGCG CCACAGCCAG TCTAGCGAGG ACCCCTTCTG CCCCGAGGGC 
ACGAGTCTCC TGTATCGTGG CTACTCCCTG CTGTACGTGC AGGGCAACGA GCGCTCCCAC 
GGTCAGGACT TGGGGACGGC GGGGAGCTGC CTGCGCAGGT TCAGCGCCAT GCCGTTCATG 
TTCTGCAACA TCAACAACGT CTGCAGCTTG GCGACGCGCA GCGACTACTC CTACTGGCTG 
TCCACACCAC AGGCCATGTC TGCGAGCATG GCGCCCGTCG CCGGGGACGA CGTCAAGCCC 
TACATCAGCA GGTGTGCGGT GTGTGAAGCT CCTGCGATGG TGGTCGCCGT GCACAGCCAG 
TCCATCCGGC TGCCCTCCTG CCCGGCCGGC TGGGACCCGC TGTGGATCGG CTACTCCTTC 
ATGATGCACA CCGGTGCGGG GGCGGAGGGT TCCGGTCAAG CCCTGGCCTC CCCCGGCTCC 
TGCCTCGAGG ACTTCCGCTC CTCCCCCTTC GTCGAATGCC ACGGAAAAGG CGCCTGCAAT 
TACTACGCCG ACTCCTACAG CTTCTGGCTG GCCACCGTGG ACGACGGCGA CATGTTTCGG 
AAGCCTCAGG AGGAGACCCT GAAAGCAGGA GACCTTGAGA CGCGGGTGAG CCGCTGTGCG 
GTGTGCATGA AGAGGACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607422.2
CDS	18..5198
Translation	MCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751906.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751906.2

ORF Insert Sequence:

ATGTGTTTTG GCTGCACGGG AGACTCCGCT TGTGCCTGCA GTGGCGTGAA AGGAGACAAG 
GGTCAACGGG GCCTTCCTGG ACTTCAGGGT CCGCCGGGGC TTCCGGGATT TCCTGGGCCG 
GAGGGGCAGG AGGGACCCAG AGGGGAGAAG GGCGCCGACG GGCCGCCGGG ACCGGCCGGT 
CCGAAAGGAA TCCGGGGTCC TCCCGGACAG CCTGGATTTC CGGGAACCCC AGGAATCCCG 
GGTCTACCAG GACAGGATGG CCCCCGGGGA CCCACGGGAC CGGCGGGGTG CAATGGGACC 
AAGGGTGAAC GAGGATTGCC TGGAAACTCT GGCTTTCCTG GGGTACAAGG TCCCCCGGGT 
CCATCTGGTA TACCGGGCGC AAAGGGAGAC CCTGGTTTGA TCCTCTCGGC GGGTCCATTC 
GGACAGATGG GAGAACCGGG CTCGCCTGGA CTTCCAGGTG TTCCTGGCAG TCAAGGCCTT 
CCGGGACTAG CAGGCCCAAC AGGACCACCC GGACCCCCAG GCTTTCAGGG GAGGCCTGGA 
CCACCGGGGC CTCCAGGGCC CAAGGGTAAC ATGGGCCTGA ACTTCCAAGG AGAGAAGGGG 
AAGAAGGGTG AACAAGGAAT GCGTGGGCAA CCGGGGCCCC CCGGCCTCAT TCGTGAGCAG 
AAGCAAGCGA GGGATTCGGA GGTGCGGAGG GGAAACAAGG GGGATCAGGG ACCACCTGGT 
CCACCTGGGA AACCTGGGGT TCCTGGGCCT CTCGGTCCTC CCGGTGGTCC GAAGGGTGAG 
AAGGGGGAGC CTGGGGAACC AGGCAAGAGG GGGAAGCCAG GGAAAGATGG TGATCCTGGA 
CCAGTCGGTT TCCCGGGTTC GAATGGAGAG CCTGGGGGTC CGGGTTTGCC CGGAACGCAT 
GGTATTACGG GCGAGAAAGG AGACCGCGGT CTCCCAGGCC TCCCGGGTGC AGTGGTGACA 
GGTTCGAATC GTATCAGTGA AGGACCAAAG GGAGAGCCTG GTTTACCTGG ACAGCTTGGG 
CAGAAAGGAG AGTCAGGTCC ACCAGGTCTT CCGGGACTTC CTGGACAGCC AGGTGCTCCA 
GGGTTTGGCT CCCCAGGCTC ACCTGGTCAG CCCGGTTTTC CTGGGGACAG AGGGCAGAGA 
GGTAATGAGG GACAGCCAGG AGTATCTTTG CCTGGACTGC CGGGGAGAGA CGGGCAGCCT 
GGGCCTCCGG GCCCCCCGGG GCCCCCAGGG CTGCCAGGAA CATCATCTGG AAATGGGCTC 
TGCCTTCGGG GGACGCCAGG GCTTCCAGGC CTTCGGGGAG AGCAAGGATT TCCAGGAGAC 
AGAGGGCAGC CGGGTGACAA AGGGGAGACC TGCATTAACT GCTTCTCCGG TGCCGATGCC 
ATTCCGGGGC CTCCGGGTCC TCCCGGTCCA TCCGGATTCA CAGGTCCTCC AGGACCACCT 
GGGATTAAAG GTGACAAAGG GATCCCAGGA ATCCCTGGAC AATCTGGCCC TCCAGGATTG 
CCTGGGGACC AAGGACCGCC AGGTGTTGCA GGACAGAAAG GAGACCCGGG AGATGCTGTC 
TCTATTGGTA GGATGAAGGG CGATAAAGGA GACACGGGCT TTCCGGGTCC ACCTGGTCTA 
CCTGGCCTGA ATGGAAGACC TGGCCAAGAC GGAATCCCAG GTTCACCTGG ACCAATAGGG 
TTACCTGGAT CTCTCTTCGT TAAAGAGGAA CATGGCCCAC CAGGGGAACC TGGAATCCCA 
GGCATTCCTG GGGACAGGGG ATCTCCAGGA GCACCAGGGT TTGGCCTTCA AGGGCCTCCA 
GGAGAGAAAG GTGTCCAAGG TGTATCGGGG CGGGCAGGTC CACCTGGTCC CCCAGGTCAA 
AAAGGTGAGC CAGGCCAGGT TGTTTCAGCG AAGGGTGTCC CTGGTCCTAT AGGGCTGAGT 
GGCGAGCCTG GCATCGCAGG ACCTCCTGGT GAGTCCGGCC AGCCTGGACA GCCTGGGTTC 
CCAGGGTTGC CTGGAGCAAA GGGAGACCTT GGACTTCCTG GCATTGGCTT ACCGGGTCCT 
CCAGGAGTCA AAGGATTCCC TGGGACACCA GGTCAGCCTG GAAGCTCTGG CAGCCCAGGA 
CCACCTGGAT TAGATGGCCT CCCTGGGCTG CCAGGCCCCC CAGGGGCCAA GGGTGACCCT 
GGGTTTGGGC TGCCAGGACC TGAAGGACCT CCTGGATTTC CTGGTAGCAA AGGAACGCAA 
GGCCCTAAAG GAGACCCTGG TTTCCCAGGG ATCCCTGGCC AGCCTGGGCG ATCAGGAGTT 
GATGGTGCTC CAGGACCCAA AGGGGATCCA GGCATTCCTG GGACCCCTGG AGCTCCTGGG 
CCCCCTGGTG CTACTTCCTT TGGGCTTCAG GGTCCTCCTG GCCCTCCAGG TCCCGCAGGT 
GCTGTAGGAT CTCCAGGGTT CCCTGGAGCG AATGGTGACA AAGGGGACCC TGGACCCCCG 
AGGCCGAACA TCCCAGGACT CCCAGGGGTT AAAGGGGAAC CGGGCTCCCC TGGACTCCCA 
GGCTCTATGG GTCCTCCTGG TTCTTCTGGT CAAACGGGTC GAGAGGGACT TCCTGGATTT 
CCTGGCCCCA AAGGAGAGAT GGGGGTGATG GGCACCGCAG GCCCTCTTGG ACCTCCAGGA 
AACTCTGGTG TTCCTGGACT CACAGGACCA AAAGGTGAGG GTGGCTCTCC CGGGCAACCT 
GGTGGACCAG GTGCTCCTGG GCCAAAGGGG GATCGAGGGG ATCCTGGTAT TCCTGGTCCC 
CCTGGCCCAA GACTTGTCGT GCAGCAGCTA CAGGGAGCAA AGGGAGAAGA GGGTCTTCCA 
GGTGCAGCCG GAGTGTCGGG ACACAAGGGT GCGCAAGGAC TCCCAGGAGA TGCTGGTCTT 
CCTGAACTTG ACGGAATACC AGGACAGCCG GGACCTCCAG GACCCAAAGG AGAGCCTGGG 
CTCCCGGGTG AACCAGGGTC CCCAGGACCA CCGGGACCAA AAGGCAATAT GGGCGAGATG 
GGATTCCCAG GTCCCAGTGG GGTGAAAGGT AACCCTGGAC AGCCAGGAAG GTCGGGCCCA 
CTTGGACAGC CAGGCACCCC TGGTTTCAGG GGAGAGAAGG GTGATCCTGG TCCTCCTGGG 
TTTGGAGTCC CAGGAAATCC AGGTACCAAG GGTGAACTGG GACAACCTGG ATTCCCTGGA 
ATCCCAGGAC CCAAAGGCCA GTCAGGTGCA CCGGGTTTAC CGGGTCTGCC TGGCAGTCCA 
GGAGAAAAGA GAAACGCAGG ACTCCCTGGA ATTCCAGGCC TTCCGGGTAT TCCTGGACCA 
AAAGGCTTCG ATGGAGCTCC AGGATCCCCA GGCGCTGATG GGCCAACAGG TCAGCCAGGA 
GAACCAGGCC GTCCTGGACC GCCTGGGCTG CCAGGAGACA AAGGCCCGCT TGGGCGCGAT 
GGCATACCTG GACCGTCTGG GGTGAAAGGA GACCCGGGTC AGCCTGGCTC GGGTCGCCTT 
GGTGTTCCAG GTCCCACAGG GTTACCAGGA CCAAAGGGAG GCGGTGGCGT CCCTGGTCTT 
CCTGGAAGCC CCGGTTTCCC TGGACGAAAG GGTGAGACGG GAACGCCCGG CATACCCGGA 
TACTCCGGAA ACGTCGGACC TCCGGGTCCC CCCGGTCCGT CACGAGCGGG GCCCAAAGGT 
AGCGCAGGGC CTCCGGGTGC CCCAGGAGGA AGCGCAGAAA ATACCTACCC GGTGGTCACC 
ATGGACAGTA TGAAAGGGCA GGCGTTAGAT CGGCAGGTCG TCTCGGCCCC GAGGGTCCAC 
GCGGCCCCCC TGGCTCGGGA GGGCTCGAGG GGCAAAAGGG CGCTGCTGGT CCCCCTGGTC 
AGCCTGGCCT GCCTGGTCAG AAGGGAGAGA GCGGAACCGC AGGCCTTCCG GGATCTCCCG 
GTCTTCCCGG GCCAGCGGGA CTCAAAGGAG ACGCCGGCCT TCCCGGACAA CCAGGGCTTC 
CAGGTCAGCG CGCGCCGCTT CGGTCTGCTC GTGCGCTCCG GTCTGTTCCT CACACTAAGA 
CGGAGACATA AATCGGAGTT TCACATGCGG GACGCCGGAG GGACCTTGAA CACTGTGCTC 
GTTGTGCTTT GCAGCCACAG CCGTGGCCTT TCGACGAAAG GAGCAGGAGG TCTGTCTGGC 
ACGCCCGGAA CACTCGGTGA CCCAGGAGAC AAAGGAGATG TCGGTCCACA AGGACCCCCG 
GGTGACCCGG GCCAGATTGC GGCCCAGATC GTGGTGAAAG GCGATCGGGG GCCCCCTGGT 
ACCCAGGGTC GCCCAGGTTC ACGTGGACCC GTGGGTCTTC CAGGTTTCGA GGGCTTGTCA 
GGTCCGCCAG GAGAACCTGG AGAACCGGGT GCTCGGGGTG CCATAGGGTT CAGCGGACCT 
TTGGGTCACA AAGGAGACGA CGGGCCACCT GGAGAGCCAG GTCAGAGGGG CTTCCCAGGC 
CCTCCCGGAC CAGATGGGCT CCGGGGACCG TCGGGCACAC CGGGGACCTC CTCGGCGGCT 
CACGGCTTTC TGGTGACGCG CCACAGCCAG TCTAGCGAGG ACCCCTTCTG CCCCGAGGGC 
ACGAGTCTCC TGTATCGTGG CTACTCCCTG CTGTACGTGC AGGGCAACGA GCGCTCCCAC 
GGTCAGGACT TGGGGACGGC GGGGAGCTGC CTGCGCAGGT TCAGCGCCAT GCCGTTCATG 
TTCTGCAACA TCAACAACGT CTGCAGCTTG GCGACGCGCA GCGACTACTC CTACTGGCTG 
TCCACACCAC AGGCCATGTC TGCGAGCATG GCGCCCGTCG CCGGGGACGA CGTCAAGCCC 
TACATCAGCA GGTGTGCGGT GTGTGAAGCT CCTGCGATGG TGGTCGCCGT GCACAGCCAG 
TCCATCCGGC TGCCCTCCTG CCCGGCCGGC TGGGACCCGC TGTGGATCGG CTACTCCTTC 
ATGATGCACA CCGGTGCGGG GGCGGAGGGT TCCGGTCAAG CCCTGGCCTC CCCCGGCTCC 
TGCCTCGAGG ACTTCCGCTC CTCCCCCTTC GTCGAATGCC ACGGAAAAGG CGCCTGCAAT 
TACTACGCCG ACTCCTACAG CTTCTGGCTG GCCACCGTGG ACGACGGCGA CATGTTTCGG 
AAGCCTCAGG AGGAGACCCT GAAAGCAGGA GACCTTGAGA CGCGGGTGAG CCGCTGTGCG 
GTGTGCATGA AGAGGACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751907.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607423.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X8, mRNA.

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CloneID	OSc131349
Clone ID Related Accession (Same CDS sequence)	XM_018751907.2
Accession Version	XM_018751907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGGAA GCCTCAGGAG GAGACCCTGA AAGCAGGAGA 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607423.2
CDS	125..5230
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQRDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMEASGGDPESRRP

Target ORF information:

RefSeq Version	XM_018751907.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751907.2

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGGAA GCCTCAGGAG GAGACCCTGA AAGCAGGAGA 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751908.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607424.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OSc131350
Clone ID Related Accession (Same CDS sequence)	XM_018751908.2
Accession Version	XM_018751908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGGTCGT 
CTCGGCCCCG AGGGTCCACG CGGCCCCCCT GGCTCGGGAG GGCTCGAGGG GCAAAAGGGC 
GCTGCTGGTC CCCCTGGTCA GCCTGGCCTG CCTGGTCAGA AGGGAGAGAG CGGAACCGCA 
GGCCTTCCGG GATCTCCCGG TCTTCCCGGG CCAGCGGGAC TCAAAGGAGA CGCCGGCCTT 
CCCGGACAAC CAGGGCTTCC AGGAGCAGGA GGTCTGTCTG GCACGCCCGG AACACTCGGT 
GACCCAGGAG ACAAAGGAGA TGTCGGTCCA CAAGGACCCC CGGGTGACCC GGGCCAGATT 
GCGGCCCAGA TCGTGGTGAA AGGCGATCGG GGGCCCCCTG GTACCCAGGG TCGCCCAGGT 
TCACGTGGAC CCGTGGGTCT TCCAGGTTTC GAGGGCTTGT CAGGTCCGCC AGGAGAACCT 
GGAGAACCGG GTGCTCGGGG TGCCATAGGG TTCAGCGGAC CTTTGGGTCA CAAAGGAGAC 
GACGGGCCAC CTGGAGAGCC AGGTCAGAGG GGCTTCCCAG GCCCTCCCGG ACCAGATGGG 
CTCCGGGGAC CGTCGGGCAC ACCGGGGACC TCCTCGGCGG CTCACGGCTT TCTGGTGACG 
CGCCACAGCC AGTCTAGCGA GGACCCCTTC TGCCCCGAGG GCACGAGTCT CCTGTATCGT 
GGCTACTCCC TGCTGTACGT GCAGGGCAAC GAGCGCTCCC ACGGTCAGGA CTTGGGGACG 
GCGGGGAGCT GCCTGCGCAG GTTCAGCGCC ATGCCGTTCA TGTTCTGCAA CATCAACAAC 
GTCTGCAGCT TGGCGACGCG CAGCGACTAC TCCTACTGGC TGTCCACACC ACAGGCCATG 
TCTGCGAGCA TGGCGCCCGT CGCCGGGGAC GACGTCAAGC CCTACATCAG CAGGTGTGCG 
GTGTGTGAAG CTCCTGCGAT GGTGGTCGCC GTGCACAGCC AGTCCATCCG GCTGCCCTCC 
TGCCCGGCCG GCTGGGACCC GCTGTGGATC GGCTACTCCT TCATGATGCA CACCGGTGCG 
GGGGCGGAGG GTTCCGGTCA AGCCCTGGCC TCCCCCGGCT CCTGCCTCGA GGACTTCCGC 
TCCTCCCCCT TCGTCGAATG CCACGGAAAA GGCGCCTGCA ATTACTACGC CGACTCCTAC 
AGCTTCTGGC TGGCCACCGT GGACGACGGC GACATGTTTC GGAAGCCTCA GGAGGAGACC 
CTGAAAGCAG GAGACCTTGA GACGCGGGTG AGCCGCTGTG CGGTGTGCAT GAAGAGGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607424.2
CDS	125..5227
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSGRLGPEGPRGPPGSGGLEGQKGAAGPPGQPGLPGQKGESGTAGLPGSPGLPGPAGLKGDAGLPGQPGLPGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751908.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751908.2

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGGTCGT 
CTCGGCCCCG AGGGTCCACG CGGCCCCCCT GGCTCGGGAG GGCTCGAGGG GCAAAAGGGC 
GCTGCTGGTC CCCCTGGTCA GCCTGGCCTG CCTGGTCAGA AGGGAGAGAG CGGAACCGCA 
GGCCTTCCGG GATCTCCCGG TCTTCCCGGG CCAGCGGGAC TCAAAGGAGA CGCCGGCCTT 
CCCGGACAAC CAGGGCTTCC AGGAGCAGGA GGTCTGTCTG GCACGCCCGG AACACTCGGT 
GACCCAGGAG ACAAAGGAGA TGTCGGTCCA CAAGGACCCC CGGGTGACCC GGGCCAGATT 
GCGGCCCAGA TCGTGGTGAA AGGCGATCGG GGGCCCCCTG GTACCCAGGG TCGCCCAGGT 
TCACGTGGAC CCGTGGGTCT TCCAGGTTTC GAGGGCTTGT CAGGTCCGCC AGGAGAACCT 
GGAGAACCGG GTGCTCGGGG TGCCATAGGG TTCAGCGGAC CTTTGGGTCA CAAAGGAGAC 
GACGGGCCAC CTGGAGAGCC AGGTCAGAGG GGCTTCCCAG GCCCTCCCGG ACCAGATGGG 
CTCCGGGGAC CGTCGGGCAC ACCGGGGACC TCCTCGGCGG CTCACGGCTT TCTGGTGACG 
CGCCACAGCC AGTCTAGCGA GGACCCCTTC TGCCCCGAGG GCACGAGTCT CCTGTATCGT 
GGCTACTCCC TGCTGTACGT GCAGGGCAAC GAGCGCTCCC ACGGTCAGGA CTTGGGGACG 
GCGGGGAGCT GCCTGCGCAG GTTCAGCGCC ATGCCGTTCA TGTTCTGCAA CATCAACAAC 
GTCTGCAGCT TGGCGACGCG CAGCGACTAC TCCTACTGGC TGTCCACACC ACAGGCCATG 
TCTGCGAGCA TGGCGCCCGT CGCCGGGGAC GACGTCAAGC CCTACATCAG CAGGTGTGCG 
GTGTGTGAAG CTCCTGCGAT GGTGGTCGCC GTGCACAGCC AGTCCATCCG GCTGCCCTCC 
TGCCCGGCCG GCTGGGACCC GCTGTGGATC GGCTACTCCT TCATGATGCA CACCGGTGCG 
GGGGCGGAGG GTTCCGGTCA AGCCCTGGCC TCCCCCGGCT CCTGCCTCGA GGACTTCCGC 
TCCTCCCCCT TCGTCGAATG CCACGGAAAA GGCGCCTGCA ATTACTACGC CGACTCCTAC 
AGCTTCTGGC TGGCCACCGT GGACGACGGC GACATGTTTC GGAAGCCTCA GGAGGAGACC 
CTGAAAGCAG GAGACCTTGA GACGCGGGTG AGCCGCTGTG CGGTGTGCAT GAAGAGGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751911.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607427.2 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OSc131352
Clone ID Related Accession (Same CDS sequence)	XM_018751911.2
Accession Version	XM_018751911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041809.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018751911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGGTC GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG 
GGGCAAAAGG GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG 
AGCGGAACCG CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA 
GACGCCGGCC TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC 
GGAACACTCG GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC 
CCGGGCCAGA TTGCGGCCCA GATCGTGGTG AAAGGCGATC GGGGGCCCCC TGGTACCCAG 
GGTCGCCCAG GTTCACGTGG ACCCGTGGGT CTTCCAGGTT TCGAGGGCTT GTCAGGTCCG 
CCAGGAGAAC CTGGAGAACC GGGTGCTCGG GGTGCCATAG GGTTCAGCGG ACCTTTGGGT 
CACAAAGGAG ACGACGGGCC ACCTGGAGAG CCAGGTCAGA GGGGCTTCCC AGGCCCTCCC 
GGACCAGATG GGCTCCGGGG ACCGTCGGGC ACACCGGGGA CCTCCTCGGC GGCTCACGGC 
TTTCTGGTGA CGCGCCACAG CCAGTCTAGC GAGGACCCCT TCTGCCCCGA GGGCACGAGT 
CTCCTGTATC GTGGCTACTC CCTGCTGTAC GTGCAGGGCA ACGAGCGCTC CCACGGTCAG 
GACTTGGGGA CGGCGGGGAG CTGCCTGCGC AGGTTCAGCG CCATGCCGTT CATGTTCTGC 
AACATCAACA ACGTCTGCAG CTTGGCGACG CGCAGCGACT ACTCCTACTG GCTGTCCACA 
CCACAGGCCA TGTCTGCGAG CATGGCGCCC GTCGCCGGGG ACGACGTCAA GCCCTACATC 
AGCAGGTGTG CGGTGTGTGA AGCTCCTGCG ATGGTGGTCG CCGTGCACAG CCAGTCCATC 
CGGCTGCCCT CCTGCCCGGC CGGCTGGGAC CCGCTGTGGA TCGGCTACTC CTTCATGATG 
CACACCGGTG CGGGGGCGGA GGGTTCCGGT CAAGCCCTGG CCTCCCCCGG CTCCTGCCTC 
GAGGACTTCC GCTCCTCCCC CTTCGTCGAA TGCCACGGAA AAGGCGCCTG CAATTACTAC 
GCCGACTCCT ACAGCTTCTG GCTGGCCACC GTGGACGACG GCGACATGTT TCGGAAGCCT 
CAGGAGGAGA CCCTGAAAGC AGGAGACCTT GAGACGCGGG TGAGCCGCTG TGCGGTGTGC 
ATGAAGAGGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607427.2
CDS	150..5204
Translation	MKTSCGTRVRNLLRVGLLLSASLGVRALQGNMCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSGRLGPEGPRGPPGSGGLEGQKGAAGPPGQPGLPGQKGESGTAGLPGSPGLPGPAGLKGDAGLPGQPGLPGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751911.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751911.2

ORF Insert Sequence:

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGGTC GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG 
GGGCAAAAGG GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG 
AGCGGAACCG CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGCGGG ACTCAAAGGA 
GACGCCGGCC TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC 
GGAACACTCG GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC 
CCGGGCCAGA TTGCGGCCCA GATCGTGGTG AAAGGCGATC GGGGGCCCCC TGGTACCCAG 
GGTCGCCCAG GTTCACGTGG ACCCGTGGGT CTTCCAGGTT TCGAGGGCTT GTCAGGTCCG 
CCAGGAGAAC CTGGAGAACC GGGTGCTCGG GGTGCCATAG GGTTCAGCGG ACCTTTGGGT 
CACAAAGGAG ACGACGGGCC ACCTGGAGAG CCAGGTCAGA GGGGCTTCCC AGGCCCTCCC 
GGACCAGATG GGCTCCGGGG ACCGTCGGGC ACACCGGGGA CCTCCTCGGC GGCTCACGGC 
TTTCTGGTGA CGCGCCACAG CCAGTCTAGC GAGGACCCCT TCTGCCCCGA GGGCACGAGT 
CTCCTGTATC GTGGCTACTC CCTGCTGTAC GTGCAGGGCA ACGAGCGCTC CCACGGTCAG 
GACTTGGGGA CGGCGGGGAG CTGCCTGCGC AGGTTCAGCG CCATGCCGTT CATGTTCTGC 
AACATCAACA ACGTCTGCAG CTTGGCGACG CGCAGCGACT ACTCCTACTG GCTGTCCACA 
CCACAGGCCA TGTCTGCGAG CATGGCGCCC GTCGCCGGGG ACGACGTCAA GCCCTACATC 
AGCAGGTGTG CGGTGTGTGA AGCTCCTGCG ATGGTGGTCG CCGTGCACAG CCAGTCCATC 
CGGCTGCCCT CCTGCCCGGC CGGCTGGGAC CCGCTGTGGA TCGGCTACTC CTTCATGATG 
CACACCGGTG CGGGGGCGGA GGGTTCCGGT CAAGCCCTGG CCTCCCCCGG CTCCTGCCTC 
GAGGACTTCC GCTCCTCCCC CTTCGTCGAA TGCCACGGAA AAGGCGCCTG CAATTACTAC 
GCCGACTCCT ACAGCTTCTG GCTGGCCACC GTGGACGACG GCGACATGTT TCGGAAGCCT 
CAGGAGGAGA CCCTGAAAGC AGGAGACCTT GAGACGCGGG TGAGCCGCTG TGCGGTGTGC 
ATGAAGAGGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751901.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607417.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X1, mRNA.

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CloneID	OSc54529
Clone ID Related Accession (Same CDS sequence)	XM_018751901.1
Accession Version	XM_018751901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607417.1
CDS	125..5446
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751901.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751901.1

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751902.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607418.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X2, mRNA.

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CloneID	OSc54530
Clone ID Related Accession (Same CDS sequence)	XM_018751902.1
Accession Version	XM_018751902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGGG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607418.1
CDS	150..5423
Translation	MKTSCGTRVRNLLRVGLLLSASLGVRALQGNMCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751902.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751902.1

ORF Insert Sequence:

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGGG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751903.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607419.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X3, mRNA.

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CloneID	OSc54531
Clone ID Related Accession (Same CDS sequence)	XM_018751903.1
Accession Version	XM_018751903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607419.1
CDS	125..5386
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQRSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751903.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751903.1

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGCAC ACCGGTGCGG GGGCGGAGGG TTCCGGTCAA 
GCCCTGGCCT CCCCCGGCTC CTGCCTCGAG GACTTCCGCT CCTCCCCCTT CGTCGAATGC 
CACGGAAAAG GCGCCTGCAA TTACTACGCC GACTCCTACA GCTTCTGGCT GGCCACCGTG 
GACGACGGCG ACATGTTTCG GAAGCCTCAG GAGGAGACCC TGAAAGCAGG AGACCTTGAG 
ACGCGGGTGA GCCGCTGTGC GGTGTGCATG AAGAGGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751904.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607420.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X4, mRNA.

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CloneID	OSc54532
Clone ID Related Accession (Same CDS sequence)	XM_018751904.1
Accession Version	XM_018751904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGGG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607420.1
CDS	150..5363
Translation	MKTSCGTRVRNLLRVGLLLSASLGVRALQGNGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751904.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751904.1

ORF Insert Sequence:

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGCAG AAAATACCTA CCCGGTGGTC ACCATGGACA GTATGAAAGG GCAGGCGTTA 
GATCGGCAGG TCGTCTCGGC CCCGAGGGTC CACGCGGCCC CCCTGGCTCG GGAGGGCTCG 
AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG 
AGAGCGGAAC CGCAGGCCTT CCGGGATCTC CCGGTCTTCC CGGGCCAGGG GGACTCAAAG 
GAGACGCCGG CCTTCCCGGA CAACCAGGGC TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG 
CTCGTGCGCT CCGGTCTGTT CCTCACACTA AGACGGAGAC ATAAATCGGA GTTTCACATG 
CGGGACGCCG GAGGGACCTT GAACACTGTG CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC 
CTTTCGACGA AAGGAGCAGG AGGTCTGTCT GGCACGCCCG GAACACTCGG TGACCCAGGA 
GACAAAGGAG ATGTCGGTCC ACAAGGACCC CCGGGTGACC CGGGCCAGAT TGCGGCCCAG 
ATCGTGGTGA AAGGCGATCG GGGGCCCCCT GGTACCCAGG GTCGCCCAGG TTCACGTGGA 
CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG TCAGGTCCGC CAGGAGAACC TGGAGAACCG 
GGTGCTCGGG GTGCCATAGG GTTCAGCGGA CCTTTGGGTC ACAAAGGAGA CGACGGGCCA 
CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA GGCCCTCCCG GACCAGATGG GCTCCGGGGA 
CCGTCGGGCA CACCGGGGAC CTCCTCGGCG GCTCACGGCT TTCTGGTGAC GCGCCACAGC 
CAGTCTAGCG AGGACCCCTT CTGCCCCGAG GGCACGAGTC TCCTGTATCG TGGCTACTCC 
CTGCTGTACG TGCAGGGCAA CGAGCGCTCC CACGGTCAGG ACTTGGGGAC GGCGGGGAGC 
TGCCTGCGCA GGTTCAGCGC CATGCCGTTC ATGTTCTGCA ACATCAACAA CGTCTGCAGC 
TTGGCGACGC GCAGCGACTA CTCCTACTGG CTGTCCACAC CACAGGCCAT GTCTGCGAGC 
ATGGCGCCCG TCGCCGGGGA CGACGTCAAG CCCTACATCA GCAGGTGTGC GGTGTGTGAA 
GCTCCTGCGA TGGTGGTCGC CGTGCACAGC CAGTCCATCC GGCTGCCCTC CTGCCCGGCC 
GGCTGGGACC CGCTGTGGAT CGGCTACTCC TTCATGATGC ACACCGGTGC GGGGGCGGAG 
GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC TCCTGCCTCG AGGACTTCCG CTCCTCCCCC 
TTCGTCGAAT GCCACGGAAA AGGCGCCTGC AATTACTACG CCGACTCCTA CAGCTTCTGG 
CTGGCCACCG TGGACGACGG CGACATGTTT CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA 
GGAGACCTTG AGACGCGGGT GAGCCGCTGT GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751905.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607421.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X5, mRNA.

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CloneID	OSc54533
Clone ID Related Accession (Same CDS sequence)	XM_018751905.1
Accession Version	XM_018751905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT TTGGCTGCAC GGGAGACTCC GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC 
AAGGGTCAAC GGGGCCTTCC TGGACTTCAG GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG 
CCGGAGGGGC AGGAGGGACC CAGAGGGGAG AAGGGCGCCG ACGGGCCGCC GGGACCGGCC 
GGTCCGAAAG GAATCCGGGG TCCTCCCGGA CAGCCTGGAT TTCCGGGAAC CCCAGGAATC 
CCGGGTCTAC CAGGACAGGA TGGCCCCCGG GGACCCACGG GACCGGCGGG GTGCAATGGG 
ACCAAGGGTG AACGAGGATT GCCTGGAAAC TCTGGCTTTC CTGGGGTACA AGGTCCCCCG 
GGTCCATCTG GTATACCGGG CGCAAAGGGA GACCCTGGTT TGATCCTCTC GGCGGGTCCA 
TTCGGACAGA TGGGAGAACC GGGCTCGCCT GGACTTCCAG GTGTTCCTGG CAGTCAAGGC 
CTTCCGGGAC TAGCAGGCCC AACAGGACCA CCCGGACCCC CAGGCTTTCA GGGGAGGCCT 
GGACCACCGG GGCCTCCAGG GCCCAAGGGT AACATGGGCC TGAACTTCCA AGGAGAGAAG 
GGGAAGAAGG GTGAACAAGG AATGCGTGGG CAACCGGGGC CCCCCGGCCT CATTCGTGAG 
CAGAAGCAAG CGAGGGATTC GGAGGTGCGG AGGGGAAACA AGGGGGATCA GGGACCACCT 
GGTCCACCTG GGAAACCTGG GGTTCCTGGG CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT 
GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG AGGGGGAAGC CAGGGAAAGA TGGTGATCCT 
GGACCAGTCG GTTTCCCGGG TTCGAATGGA GAGCCTGGGG GTCCGGGTTT GCCCGGAACG 
CATGGTATTA CGGGCGAGAA AGGAGACCGC GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG 
ACAGGTTCGA ATCGTATCAG TGAAGGACCA AAGGGAGAGC CTGGTTTACC TGGACAGCTT 
GGGCAGAAAG GAGAGTCAGG TCCACCAGGT CTTCCGGGAC TTCCTGGACA GCCAGGTGCT 
CCAGGGTTTG GCTCCCCAGG CTCACCTGGT CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG 
AGAGGTAATG AGGGACAGCC AGGAGTATCT TTGCCTGGAC TGCCGGGGAG AGACGGGCAG 
CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA GGGCTGCCAG GAACATCATC TGGAAATGGG 
CTCTGCCTTC GGGGGACGCC AGGGCTTCCA GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA 
GACAGAGGGC AGCCGGGTGA CAAAGGGGAG ACCTGCATTA ACTGCTTCTC CGGTGCCGAT 
GCCATTCCGG GGCCTCCGGG TCCTCCCGGT CCATCCGGAT TCACAGGTCC TCCAGGACCA 
CCTGGGATTA AAGGTGACAA AGGGATCCCA GGAATCCCTG GACAATCTGG CCCTCCAGGA 
TTGCCTGGGG ACCAAGGACC GCCAGGTGTT GCAGGACAGA AAGGAGACCC GGGAGATGCT 
GTCTCTATTG GTAGGATGAA GGGCGATAAA GGAGACACGG GCTTTCCGGG TCCACCTGGT 
CTACCTGGCC TGAATGGAAG ACCTGGCCAA GACGGAATCC CAGGTTCACC TGGACCAATA 
GGGTTACCTG GATCTCTCTT CGTTAAAGAG GAACATGGCC CACCAGGGGA ACCTGGAATC 
CCAGGCATTC CTGGGGACAG GGGATCTCCA GGAGCACCAG GGTTTGGCCT TCAAGGGCCT 
CCAGGAGAGA AAGGTGTCCA AGGTGTATCG GGGCGGGCAG GTCCACCTGG TCCCCCAGGT 
CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA GCGAAGGGTG TCCCTGGTCC TATAGGGCTG 
AGTGGCGAGC CTGGCATCGC AGGACCTCCT GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG 
TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC CTTGGACTTC CTGGCATTGG CTTACCGGGT 
CCTCCAGGAG TCAAAGGATT CCCTGGGACA CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA 
GGACCACCTG GATTAGATGG CCTCCCTGGG CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC 
CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA CCTCCTGGAT TTCCTGGTAG CAAAGGAACG 
CAAGGCCCTA AAGGAGACCC TGGTTTCCCA GGGATCCCTG GCCAGCCTGG GCGATCAGGA 
GTTGATGGTG CTCCAGGACC CAAAGGGGAT CCAGGCATTC CTGGGACCCC TGGAGCTCCT 
GGGCCCCCTG GTGCTACTTC CTTTGGGCTT CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA 
GGTGCTGTAG GATCTCCAGG GTTCCCTGGA GCGAATGGTG ACAAAGGGGA CCCTGGACCC 
CCGAGGCCGA ACATCCCAGG ACTCCCAGGG GTTAAAGGGG AACCGGGCTC CCCTGGACTC 
CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT GGTCAAACGG GTCGAGAGGG ACTTCCTGGA 
TTTCCTGGCC CCAAAGGAGA GATGGGGGTG ATGGGCACCG CAGGCCCTCT TGGACCTCCA 
GGAAACTCTG GTGTTCCTGG ACTCACAGGA CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA 
CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG GGGGATCGAG GGGATCCTGG TATTCCTGGT 
CCCCCTGGCC CAAGACTTGT CGTGCAGCAG CTACAGGGAG CAAAGGGAGA AGAGGGTCTT 
CCAGGTGCAG CCGGAGTGTC GGGACACAAG GGTGCGCAAG GACTCCCAGG AGATGCTGGT 
CTTCCTGAAC TTGACGGAAT ACCAGGACAG CCGGGACCTC CAGGACCCAA AGGAGAGCCT 
GGGCTCCCGG GTGAACCAGG GTCCCCAGGA CCACCGGGAC CAAAAGGCAA TATGGGCGAG 
ATGGGATTCC CAGGTCCCAG TGGGGTGAAA GGTAACCCTG GACAGCCAGG AAGGTCGGGC 
CCACTTGGAC AGCCAGGCAC CCCTGGTTTC AGGGGAGAGA AGGGTGATCC TGGTCCTCCT 
GGGTTTGGAG TCCCAGGAAA TCCAGGTACC AAGGGTGAAC TGGGACAACC TGGATTCCCT 
GGAATCCCAG GACCCAAAGG CCAGTCAGGT GCACCGGGTT TACCGGGTCT GCCTGGCAGT 
CCAGGAGAAA AGAGAAACGC AGGACTCCCT GGAATTCCAG GCCTTCCGGG TATTCCTGGA 
CCAAAAGGCT TCGATGGAGC TCCAGGATCC CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA 
GGAGAACCAG GCCGTCCTGG ACCGCCTGGG CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC 
GATGGCATAC CTGGACCGTC TGGGGTGAAA GGAGACCCGG GTCAGCCTGG CTCGGGTCGC 
CTTGGTGTTC CAGGTCCCAC AGGGTTACCA GGACCAAAGG GAGGCGGTGG CGTCCCTGGT 
CTTCCTGGAA GCCCCGGTTT CCCTGGACGA AAGGGTGAGA CGGGAACGCC CGGCATACCC 
GGATACTCCG GAAACGTCGG ACCTCCGGGT CCCCCCGGTC CGTCACGAGC GGGGCCCAAA 
GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA GGAAGCGCAG AAAATACCTA CCCGGTGGTC 
ACCATGGACA GTATGAAAGG GCAGGCGTTA GATCGGCAGG TCGTCTCGGC CCCGAGGGTC 
CACGCGGCCC CCCTGGCTCG GGAGGGCTCG AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG 
GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG AGAGCGGAAC CGCAGGCCTT CCGGGATCTC 
CCGGTCTTCC CGGGCCAGGG GGACTCAAAG GAGACGCCGG CCTTCCCGGA CAACCAGGGC 
TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG CTCGTGCGCT CCGGTCTGTT CCTCACACTA 
AGACGGAGAC ATAAATCGGA GTTTCACATG CGGGACGCCG GAGGGACCTT GAACACTGTG 
CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC CTTTCGACGA AAGGAGCAGG AGGTCTGTCT 
GGCACGCCCG GAACACTCGG TGACCCAGGA GACAAAGGAG ATGTCGGTCC ACAAGGACCC 
CCGGGTGACC CGGGCCAGAT TGCGGCCCAG ATCGTGGTGA AAGGCGATCG GGGGCCCCCT 
GGTACCCAGG GTCGCCCAGG TTCACGTGGA CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG 
TCAGGTCCGC CAGGAGAACC TGGAGAACCG GGTGCTCGGG GTGCCATAGG GTTCAGCGGA 
CCTTTGGGTC ACAAAGGAGA CGACGGGCCA CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA 
GGCCCTCCCG GACCAGATGG GCTCCGGGGA CCGTCGGGCA CACCGGGGAC CTCCTCGGCG 
GCTCACGGCT TTCTGGTGAC GCGCCACAGC CAGTCTAGCG AGGACCCCTT CTGCCCCGAG 
GGCACGAGTC TCCTGTATCG TGGCTACTCC CTGCTGTACG TGCAGGGCAA CGAGCGCTCC 
CACGGTCAGG ACTTGGGGAC GGCGGGGAGC TGCCTGCGCA GGTTCAGCGC CATGCCGTTC 
ATGTTCTGCA ACATCAACAA CGTCTGCAGC TTGGCGACGC GCAGCGACTA CTCCTACTGG 
CTGTCCACAC CACAGGCCAT GTCTGCGAGC ATGGCGCCCG TCGCCGGGGA CGACGTCAAG 
CCCTACATCA GCAGGTGTGC GGTGTGTGAA GCTCCTGCGA TGGTGGTCGC CGTGCACAGC 
CAGTCCATCC GGCTGCCCTC CTGCCCGGCC GGCTGGGACC CGCTGTGGAT CGGCTACTCC 
TTCATGATGC ACACCGGTGC GGGGGCGGAG GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC 
TCCTGCCTCG AGGACTTCCG CTCCTCCCCC TTCGTCGAAT GCCACGGAAA AGGCGCCTGC 
AATTACTACG CCGACTCCTA CAGCTTCTGG CTGGCCACCG TGGACGACGG CGACATGTTT 
CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA GGAGACCTTG AGACGCGGGT GAGCCGCTGT 
GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607421.1
CDS	34..5217
Translation	MMCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751905.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751905.1

ORF Insert Sequence:

ATGATGTGTT TTGGCTGCAC GGGAGACTCC GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC 
AAGGGTCAAC GGGGCCTTCC TGGACTTCAG GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG 
CCGGAGGGGC AGGAGGGACC CAGAGGGGAG AAGGGCGCCG ACGGGCCGCC GGGACCGGCC 
GGTCCGAAAG GAATCCGGGG TCCTCCCGGA CAGCCTGGAT TTCCGGGAAC CCCAGGAATC 
CCGGGTCTAC CAGGACAGGA TGGCCCCCGG GGACCCACGG GACCGGCGGG GTGCAATGGG 
ACCAAGGGTG AACGAGGATT GCCTGGAAAC TCTGGCTTTC CTGGGGTACA AGGTCCCCCG 
GGTCCATCTG GTATACCGGG CGCAAAGGGA GACCCTGGTT TGATCCTCTC GGCGGGTCCA 
TTCGGACAGA TGGGAGAACC GGGCTCGCCT GGACTTCCAG GTGTTCCTGG CAGTCAAGGC 
CTTCCGGGAC TAGCAGGCCC AACAGGACCA CCCGGACCCC CAGGCTTTCA GGGGAGGCCT 
GGACCACCGG GGCCTCCAGG GCCCAAGGGT AACATGGGCC TGAACTTCCA AGGAGAGAAG 
GGGAAGAAGG GTGAACAAGG AATGCGTGGG CAACCGGGGC CCCCCGGCCT CATTCGTGAG 
CAGAAGCAAG CGAGGGATTC GGAGGTGCGG AGGGGAAACA AGGGGGATCA GGGACCACCT 
GGTCCACCTG GGAAACCTGG GGTTCCTGGG CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT 
GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG AGGGGGAAGC CAGGGAAAGA TGGTGATCCT 
GGACCAGTCG GTTTCCCGGG TTCGAATGGA GAGCCTGGGG GTCCGGGTTT GCCCGGAACG 
CATGGTATTA CGGGCGAGAA AGGAGACCGC GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG 
ACAGGTTCGA ATCGTATCAG TGAAGGACCA AAGGGAGAGC CTGGTTTACC TGGACAGCTT 
GGGCAGAAAG GAGAGTCAGG TCCACCAGGT CTTCCGGGAC TTCCTGGACA GCCAGGTGCT 
CCAGGGTTTG GCTCCCCAGG CTCACCTGGT CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG 
AGAGGTAATG AGGGACAGCC AGGAGTATCT TTGCCTGGAC TGCCGGGGAG AGACGGGCAG 
CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA GGGCTGCCAG GAACATCATC TGGAAATGGG 
CTCTGCCTTC GGGGGACGCC AGGGCTTCCA GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA 
GACAGAGGGC AGCCGGGTGA CAAAGGGGAG ACCTGCATTA ACTGCTTCTC CGGTGCCGAT 
GCCATTCCGG GGCCTCCGGG TCCTCCCGGT CCATCCGGAT TCACAGGTCC TCCAGGACCA 
CCTGGGATTA AAGGTGACAA AGGGATCCCA GGAATCCCTG GACAATCTGG CCCTCCAGGA 
TTGCCTGGGG ACCAAGGACC GCCAGGTGTT GCAGGACAGA AAGGAGACCC GGGAGATGCT 
GTCTCTATTG GTAGGATGAA GGGCGATAAA GGAGACACGG GCTTTCCGGG TCCACCTGGT 
CTACCTGGCC TGAATGGAAG ACCTGGCCAA GACGGAATCC CAGGTTCACC TGGACCAATA 
GGGTTACCTG GATCTCTCTT CGTTAAAGAG GAACATGGCC CACCAGGGGA ACCTGGAATC 
CCAGGCATTC CTGGGGACAG GGGATCTCCA GGAGCACCAG GGTTTGGCCT TCAAGGGCCT 
CCAGGAGAGA AAGGTGTCCA AGGTGTATCG GGGCGGGCAG GTCCACCTGG TCCCCCAGGT 
CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA GCGAAGGGTG TCCCTGGTCC TATAGGGCTG 
AGTGGCGAGC CTGGCATCGC AGGACCTCCT GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG 
TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC CTTGGACTTC CTGGCATTGG CTTACCGGGT 
CCTCCAGGAG TCAAAGGATT CCCTGGGACA CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA 
GGACCACCTG GATTAGATGG CCTCCCTGGG CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC 
CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA CCTCCTGGAT TTCCTGGTAG CAAAGGAACG 
CAAGGCCCTA AAGGAGACCC TGGTTTCCCA GGGATCCCTG GCCAGCCTGG GCGATCAGGA 
GTTGATGGTG CTCCAGGACC CAAAGGGGAT CCAGGCATTC CTGGGACCCC TGGAGCTCCT 
GGGCCCCCTG GTGCTACTTC CTTTGGGCTT CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA 
GGTGCTGTAG GATCTCCAGG GTTCCCTGGA GCGAATGGTG ACAAAGGGGA CCCTGGACCC 
CCGAGGCCGA ACATCCCAGG ACTCCCAGGG GTTAAAGGGG AACCGGGCTC CCCTGGACTC 
CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT GGTCAAACGG GTCGAGAGGG ACTTCCTGGA 
TTTCCTGGCC CCAAAGGAGA GATGGGGGTG ATGGGCACCG CAGGCCCTCT TGGACCTCCA 
GGAAACTCTG GTGTTCCTGG ACTCACAGGA CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA 
CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG GGGGATCGAG GGGATCCTGG TATTCCTGGT 
CCCCCTGGCC CAAGACTTGT CGTGCAGCAG CTACAGGGAG CAAAGGGAGA AGAGGGTCTT 
CCAGGTGCAG CCGGAGTGTC GGGACACAAG GGTGCGCAAG GACTCCCAGG AGATGCTGGT 
CTTCCTGAAC TTGACGGAAT ACCAGGACAG CCGGGACCTC CAGGACCCAA AGGAGAGCCT 
GGGCTCCCGG GTGAACCAGG GTCCCCAGGA CCACCGGGAC CAAAAGGCAA TATGGGCGAG 
ATGGGATTCC CAGGTCCCAG TGGGGTGAAA GGTAACCCTG GACAGCCAGG AAGGTCGGGC 
CCACTTGGAC AGCCAGGCAC CCCTGGTTTC AGGGGAGAGA AGGGTGATCC TGGTCCTCCT 
GGGTTTGGAG TCCCAGGAAA TCCAGGTACC AAGGGTGAAC TGGGACAACC TGGATTCCCT 
GGAATCCCAG GACCCAAAGG CCAGTCAGGT GCACCGGGTT TACCGGGTCT GCCTGGCAGT 
CCAGGAGAAA AGAGAAACGC AGGACTCCCT GGAATTCCAG GCCTTCCGGG TATTCCTGGA 
CCAAAAGGCT TCGATGGAGC TCCAGGATCC CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA 
GGAGAACCAG GCCGTCCTGG ACCGCCTGGG CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC 
GATGGCATAC CTGGACCGTC TGGGGTGAAA GGAGACCCGG GTCAGCCTGG CTCGGGTCGC 
CTTGGTGTTC CAGGTCCCAC AGGGTTACCA GGACCAAAGG GAGGCGGTGG CGTCCCTGGT 
CTTCCTGGAA GCCCCGGTTT CCCTGGACGA AAGGGTGAGA CGGGAACGCC CGGCATACCC 
GGATACTCCG GAAACGTCGG ACCTCCGGGT CCCCCCGGTC CGTCACGAGC GGGGCCCAAA 
GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA GGAAGCGCAG AAAATACCTA CCCGGTGGTC 
ACCATGGACA GTATGAAAGG GCAGGCGTTA GATCGGCAGG TCGTCTCGGC CCCGAGGGTC 
CACGCGGCCC CCCTGGCTCG GGAGGGCTCG AGGGGCAAAA GGGCGCTGCT GGTCCCCCTG 
GTCAGCCTGG CCTGCCTGGT CAGAAGGGAG AGAGCGGAAC CGCAGGCCTT CCGGGATCTC 
CCGGTCTTCC CGGGCCAGGG GGACTCAAAG GAGACGCCGG CCTTCCCGGA CAACCAGGGC 
TTCCAGGTCA GCGCGCGCCG CTTCGGTCTG CTCGTGCGCT CCGGTCTGTT CCTCACACTA 
AGACGGAGAC ATAAATCGGA GTTTCACATG CGGGACGCCG GAGGGACCTT GAACACTGTG 
CTCGTTGTGC TTTGCAGCCA CAGCCGTGGC CTTTCGACGA AAGGAGCAGG AGGTCTGTCT 
GGCACGCCCG GAACACTCGG TGACCCAGGA GACAAAGGAG ATGTCGGTCC ACAAGGACCC 
CCGGGTGACC CGGGCCAGAT TGCGGCCCAG ATCGTGGTGA AAGGCGATCG GGGGCCCCCT 
GGTACCCAGG GTCGCCCAGG TTCACGTGGA CCCGTGGGTC TTCCAGGTTT CGAGGGCTTG 
TCAGGTCCGC CAGGAGAACC TGGAGAACCG GGTGCTCGGG GTGCCATAGG GTTCAGCGGA 
CCTTTGGGTC ACAAAGGAGA CGACGGGCCA CCTGGAGAGC CAGGTCAGAG GGGCTTCCCA 
GGCCCTCCCG GACCAGATGG GCTCCGGGGA CCGTCGGGCA CACCGGGGAC CTCCTCGGCG 
GCTCACGGCT TTCTGGTGAC GCGCCACAGC CAGTCTAGCG AGGACCCCTT CTGCCCCGAG 
GGCACGAGTC TCCTGTATCG TGGCTACTCC CTGCTGTACG TGCAGGGCAA CGAGCGCTCC 
CACGGTCAGG ACTTGGGGAC GGCGGGGAGC TGCCTGCGCA GGTTCAGCGC CATGCCGTTC 
ATGTTCTGCA ACATCAACAA CGTCTGCAGC TTGGCGACGC GCAGCGACTA CTCCTACTGG 
CTGTCCACAC CACAGGCCAT GTCTGCGAGC ATGGCGCCCG TCGCCGGGGA CGACGTCAAG 
CCCTACATCA GCAGGTGTGC GGTGTGTGAA GCTCCTGCGA TGGTGGTCGC CGTGCACAGC 
CAGTCCATCC GGCTGCCCTC CTGCCCGGCC GGCTGGGACC CGCTGTGGAT CGGCTACTCC 
TTCATGATGC ACACCGGTGC GGGGGCGGAG GGTTCCGGTC AAGCCCTGGC CTCCCCCGGC 
TCCTGCCTCG AGGACTTCCG CTCCTCCCCC TTCGTCGAAT GCCACGGAAA AGGCGCCTGC 
AATTACTACG CCGACTCCTA CAGCTTCTGG CTGGCCACCG TGGACGACGG CGACATGTTT 
CGGAAGCCTC AGGAGGAGAC CCTGAAAGCA GGAGACCTTG AGACGCGGGT GAGCCGCTGT 
GCGGTGTGCA TGAAGAGGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751906.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607422.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X6, mRNA.

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CloneID	OSc54534
Clone ID Related Accession (Same CDS sequence)	XM_018751906.1
Accession Version	XM_018751906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTTG GCTGCACGGG AGACTCCGCT TGTGCCTGCA GTGGCGTGAA AGGAGACAAG 
GGTCAACGGG GCCTTCCTGG ACTTCAGGGT CCGCCGGGGC TTCCGGGATT TCCTGGGCCG 
GAGGGGCAGG AGGGACCCAG AGGGGAGAAG GGCGCCGACG GGCCGCCGGG ACCGGCCGGT 
CCGAAAGGAA TCCGGGGTCC TCCCGGACAG CCTGGATTTC CGGGAACCCC AGGAATCCCG 
GGTCTACCAG GACAGGATGG CCCCCGGGGA CCCACGGGAC CGGCGGGGTG CAATGGGACC 
AAGGGTGAAC GAGGATTGCC TGGAAACTCT GGCTTTCCTG GGGTACAAGG TCCCCCGGGT 
CCATCTGGTA TACCGGGCGC AAAGGGAGAC CCTGGTTTGA TCCTCTCGGC GGGTCCATTC 
GGACAGATGG GAGAACCGGG CTCGCCTGGA CTTCCAGGTG TTCCTGGCAG TCAAGGCCTT 
CCGGGACTAG CAGGCCCAAC AGGACCACCC GGACCCCCAG GCTTTCAGGG GAGGCCTGGA 
CCACCGGGGC CTCCAGGGCC CAAGGGTAAC ATGGGCCTGA ACTTCCAAGG AGAGAAGGGG 
AAGAAGGGTG AACAAGGAAT GCGTGGGCAA CCGGGGCCCC CCGGCCTCAT TCGTGAGCAG 
AAGCAAGCGA GGGATTCGGA GGTGCGGAGG GGAAACAAGG GGGATCAGGG ACCACCTGGT 
CCACCTGGGA AACCTGGGGT TCCTGGGCCT CTCGGTCCTC CCGGTGGTCC GAAGGGTGAG 
AAGGGGGAGC CTGGGGAACC AGGCAAGAGG GGGAAGCCAG GGAAAGATGG TGATCCTGGA 
CCAGTCGGTT TCCCGGGTTC GAATGGAGAG CCTGGGGGTC CGGGTTTGCC CGGAACGCAT 
GGTATTACGG GCGAGAAAGG AGACCGCGGT CTCCCAGGCC TCCCGGGTGC AGTGGTGACA 
GGTTCGAATC GTATCAGTGA AGGACCAAAG GGAGAGCCTG GTTTACCTGG ACAGCTTGGG 
CAGAAAGGAG AGTCAGGTCC ACCAGGTCTT CCGGGACTTC CTGGACAGCC AGGTGCTCCA 
GGGTTTGGCT CCCCAGGCTC ACCTGGTCAG CCCGGTTTTC CTGGGGACAG AGGGCAGAGA 
GGTAATGAGG GACAGCCAGG AGTATCTTTG CCTGGACTGC CGGGGAGAGA CGGGCAGCCT 
GGGCCTCCGG GCCCCCCGGG GCCCCCAGGG CTGCCAGGAA CATCATCTGG AAATGGGCTC 
TGCCTTCGGG GGACGCCAGG GCTTCCAGGC CTTCGGGGAG AGCAAGGATT TCCAGGAGAC 
AGAGGGCAGC CGGGTGACAA AGGGGAGACC TGCATTAACT GCTTCTCCGG TGCCGATGCC 
ATTCCGGGGC CTCCGGGTCC TCCCGGTCCA TCCGGATTCA CAGGTCCTCC AGGACCACCT 
GGGATTAAAG GTGACAAAGG GATCCCAGGA ATCCCTGGAC AATCTGGCCC TCCAGGATTG 
CCTGGGGACC AAGGACCGCC AGGTGTTGCA GGACAGAAAG GAGACCCGGG AGATGCTGTC 
TCTATTGGTA GGATGAAGGG CGATAAAGGA GACACGGGCT TTCCGGGTCC ACCTGGTCTA 
CCTGGCCTGA ATGGAAGACC TGGCCAAGAC GGAATCCCAG GTTCACCTGG ACCAATAGGG 
TTACCTGGAT CTCTCTTCGT TAAAGAGGAA CATGGCCCAC CAGGGGAACC TGGAATCCCA 
GGCATTCCTG GGGACAGGGG ATCTCCAGGA GCACCAGGGT TTGGCCTTCA AGGGCCTCCA 
GGAGAGAAAG GTGTCCAAGG TGTATCGGGG CGGGCAGGTC CACCTGGTCC CCCAGGTCAA 
AAAGGTGAGC CAGGCCAGGT TGTTTCAGCG AAGGGTGTCC CTGGTCCTAT AGGGCTGAGT 
GGCGAGCCTG GCATCGCAGG ACCTCCTGGT GAGTCCGGCC AGCCTGGACA GCCTGGGTTC 
CCAGGGTTGC CTGGAGCAAA GGGAGACCTT GGACTTCCTG GCATTGGCTT ACCGGGTCCT 
CCAGGAGTCA AAGGATTCCC TGGGACACCA GGTCAGCCTG GAAGCTCTGG CAGCCCAGGA 
CCACCTGGAT TAGATGGCCT CCCTGGGCTG CCAGGCCCCC CAGGGGCCAA GGGTGACCCT 
GGGTTTGGGC TGCCAGGACC TGAAGGACCT CCTGGATTTC CTGGTAGCAA AGGAACGCAA 
GGCCCTAAAG GAGACCCTGG TTTCCCAGGG ATCCCTGGCC AGCCTGGGCG ATCAGGAGTT 
GATGGTGCTC CAGGACCCAA AGGGGATCCA GGCATTCCTG GGACCCCTGG AGCTCCTGGG 
CCCCCTGGTG CTACTTCCTT TGGGCTTCAG GGTCCTCCTG GCCCTCCAGG TCCCGCAGGT 
GCTGTAGGAT CTCCAGGGTT CCCTGGAGCG AATGGTGACA AAGGGGACCC TGGACCCCCG 
AGGCCGAACA TCCCAGGACT CCCAGGGGTT AAAGGGGAAC CGGGCTCCCC TGGACTCCCA 
GGCTCTATGG GTCCTCCTGG TTCTTCTGGT CAAACGGGTC GAGAGGGACT TCCTGGATTT 
CCTGGCCCCA AAGGAGAGAT GGGGGTGATG GGCACCGCAG GCCCTCTTGG ACCTCCAGGA 
AACTCTGGTG TTCCTGGACT CACAGGACCA AAAGGTGAGG GTGGCTCTCC CGGGCAACCT 
GGTGGACCAG GTGCTCCTGG GCCAAAGGGG GATCGAGGGG ATCCTGGTAT TCCTGGTCCC 
CCTGGCCCAA GACTTGTCGT GCAGCAGCTA CAGGGAGCAA AGGGAGAAGA GGGTCTTCCA 
GGTGCAGCCG GAGTGTCGGG ACACAAGGGT GCGCAAGGAC TCCCAGGAGA TGCTGGTCTT 
CCTGAACTTG ACGGAATACC AGGACAGCCG GGACCTCCAG GACCCAAAGG AGAGCCTGGG 
CTCCCGGGTG AACCAGGGTC CCCAGGACCA CCGGGACCAA AAGGCAATAT GGGCGAGATG 
GGATTCCCAG GTCCCAGTGG GGTGAAAGGT AACCCTGGAC AGCCAGGAAG GTCGGGCCCA 
CTTGGACAGC CAGGCACCCC TGGTTTCAGG GGAGAGAAGG GTGATCCTGG TCCTCCTGGG 
TTTGGAGTCC CAGGAAATCC AGGTACCAAG GGTGAACTGG GACAACCTGG ATTCCCTGGA 
ATCCCAGGAC CCAAAGGCCA GTCAGGTGCA CCGGGTTTAC CGGGTCTGCC TGGCAGTCCA 
GGAGAAAAGA GAAACGCAGG ACTCCCTGGA ATTCCAGGCC TTCCGGGTAT TCCTGGACCA 
AAAGGCTTCG ATGGAGCTCC AGGATCCCCA GGCGCTGATG GGCCAACAGG TCAGCCAGGA 
GAACCAGGCC GTCCTGGACC GCCTGGGCTG CCAGGAGACA AAGGCCCGCT TGGGCGCGAT 
GGCATACCTG GACCGTCTGG GGTGAAAGGA GACCCGGGTC AGCCTGGCTC GGGTCGCCTT 
GGTGTTCCAG GTCCCACAGG GTTACCAGGA CCAAAGGGAG GCGGTGGCGT CCCTGGTCTT 
CCTGGAAGCC CCGGTTTCCC TGGACGAAAG GGTGAGACGG GAACGCCCGG CATACCCGGA 
TACTCCGGAA ACGTCGGACC TCCGGGTCCC CCCGGTCCGT CACGAGCGGG GCCCAAAGGT 
AGCGCAGGGC CTCCGGGTGC CCCAGGAGGA AGCGCAGAAA ATACCTACCC GGTGGTCACC 
ATGGACAGTA TGAAAGGGCA GGCGTTAGAT CGGCAGGTCG TCTCGGCCCC GAGGGTCCAC 
GCGGCCCCCC TGGCTCGGGA GGGCTCGAGG GGCAAAAGGG CGCTGCTGGT CCCCCTGGTC 
AGCCTGGCCT GCCTGGTCAG AAGGGAGAGA GCGGAACCGC AGGCCTTCCG GGATCTCCCG 
GTCTTCCCGG GCCAGGGGGA CTCAAAGGAG ACGCCGGCCT TCCCGGACAA CCAGGGCTTC 
CAGGTCAGCG CGCGCCGCTT CGGTCTGCTC GTGCGCTCCG GTCTGTTCCT CACACTAAGA 
CGGAGACATA AATCGGAGTT TCACATGCGG GACGCCGGAG GGACCTTGAA CACTGTGCTC 
GTTGTGCTTT GCAGCCACAG CCGTGGCCTT TCGACGAAAG GAGCAGGAGG TCTGTCTGGC 
ACGCCCGGAA CACTCGGTGA CCCAGGAGAC AAAGGAGATG TCGGTCCACA AGGACCCCCG 
GGTGACCCGG GCCAGATTGC GGCCCAGATC GTGGTGAAAG GCGATCGGGG GCCCCCTGGT 
ACCCAGGGTC GCCCAGGTTC ACGTGGACCC GTGGGTCTTC CAGGTTTCGA GGGCTTGTCA 
GGTCCGCCAG GAGAACCTGG AGAACCGGGT GCTCGGGGTG CCATAGGGTT CAGCGGACCT 
TTGGGTCACA AAGGAGACGA CGGGCCACCT GGAGAGCCAG GTCAGAGGGG CTTCCCAGGC 
CCTCCCGGAC CAGATGGGCT CCGGGGACCG TCGGGCACAC CGGGGACCTC CTCGGCGGCT 
CACGGCTTTC TGGTGACGCG CCACAGCCAG TCTAGCGAGG ACCCCTTCTG CCCCGAGGGC 
ACGAGTCTCC TGTATCGTGG CTACTCCCTG CTGTACGTGC AGGGCAACGA GCGCTCCCAC 
GGTCAGGACT TGGGGACGGC GGGGAGCTGC CTGCGCAGGT TCAGCGCCAT GCCGTTCATG 
TTCTGCAACA TCAACAACGT CTGCAGCTTG GCGACGCGCA GCGACTACTC CTACTGGCTG 
TCCACACCAC AGGCCATGTC TGCGAGCATG GCGCCCGTCG CCGGGGACGA CGTCAAGCCC 
TACATCAGCA GGTGTGCGGT GTGTGAAGCT CCTGCGATGG TGGTCGCCGT GCACAGCCAG 
TCCATCCGGC TGCCCTCCTG CCCGGCCGGC TGGGACCCGC TGTGGATCGG CTACTCCTTC 
ATGATGCACA CCGGTGCGGG GGCGGAGGGT TCCGGTCAAG CCCTGGCCTC CCCCGGCTCC 
TGCCTCGAGG ACTTCCGCTC CTCCCCCTTC GTCGAATGCC ACGGAAAAGG CGCCTGCAAT 
TACTACGCCG ACTCCTACAG CTTCTGGCTG GCCACCGTGG ACGACGGCGA CATGTTTCGG 
AAGCCTCAGG AGGAGACCCT GAAAGCAGGA GACCTTGAGA CGCGGGTGAG CCGCTGTGCG 
GTGTGCATGA AGAGGACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607422.1
CDS	18..5198
Translation	MCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751906.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751906.1

ORF Insert Sequence:

ATGTGTTTTG GCTGCACGGG AGACTCCGCT TGTGCCTGCA GTGGCGTGAA AGGAGACAAG 
GGTCAACGGG GCCTTCCTGG ACTTCAGGGT CCGCCGGGGC TTCCGGGATT TCCTGGGCCG 
GAGGGGCAGG AGGGACCCAG AGGGGAGAAG GGCGCCGACG GGCCGCCGGG ACCGGCCGGT 
CCGAAAGGAA TCCGGGGTCC TCCCGGACAG CCTGGATTTC CGGGAACCCC AGGAATCCCG 
GGTCTACCAG GACAGGATGG CCCCCGGGGA CCCACGGGAC CGGCGGGGTG CAATGGGACC 
AAGGGTGAAC GAGGATTGCC TGGAAACTCT GGCTTTCCTG GGGTACAAGG TCCCCCGGGT 
CCATCTGGTA TACCGGGCGC AAAGGGAGAC CCTGGTTTGA TCCTCTCGGC GGGTCCATTC 
GGACAGATGG GAGAACCGGG CTCGCCTGGA CTTCCAGGTG TTCCTGGCAG TCAAGGCCTT 
CCGGGACTAG CAGGCCCAAC AGGACCACCC GGACCCCCAG GCTTTCAGGG GAGGCCTGGA 
CCACCGGGGC CTCCAGGGCC CAAGGGTAAC ATGGGCCTGA ACTTCCAAGG AGAGAAGGGG 
AAGAAGGGTG AACAAGGAAT GCGTGGGCAA CCGGGGCCCC CCGGCCTCAT TCGTGAGCAG 
AAGCAAGCGA GGGATTCGGA GGTGCGGAGG GGAAACAAGG GGGATCAGGG ACCACCTGGT 
CCACCTGGGA AACCTGGGGT TCCTGGGCCT CTCGGTCCTC CCGGTGGTCC GAAGGGTGAG 
AAGGGGGAGC CTGGGGAACC AGGCAAGAGG GGGAAGCCAG GGAAAGATGG TGATCCTGGA 
CCAGTCGGTT TCCCGGGTTC GAATGGAGAG CCTGGGGGTC CGGGTTTGCC CGGAACGCAT 
GGTATTACGG GCGAGAAAGG AGACCGCGGT CTCCCAGGCC TCCCGGGTGC AGTGGTGACA 
GGTTCGAATC GTATCAGTGA AGGACCAAAG GGAGAGCCTG GTTTACCTGG ACAGCTTGGG 
CAGAAAGGAG AGTCAGGTCC ACCAGGTCTT CCGGGACTTC CTGGACAGCC AGGTGCTCCA 
GGGTTTGGCT CCCCAGGCTC ACCTGGTCAG CCCGGTTTTC CTGGGGACAG AGGGCAGAGA 
GGTAATGAGG GACAGCCAGG AGTATCTTTG CCTGGACTGC CGGGGAGAGA CGGGCAGCCT 
GGGCCTCCGG GCCCCCCGGG GCCCCCAGGG CTGCCAGGAA CATCATCTGG AAATGGGCTC 
TGCCTTCGGG GGACGCCAGG GCTTCCAGGC CTTCGGGGAG AGCAAGGATT TCCAGGAGAC 
AGAGGGCAGC CGGGTGACAA AGGGGAGACC TGCATTAACT GCTTCTCCGG TGCCGATGCC 
ATTCCGGGGC CTCCGGGTCC TCCCGGTCCA TCCGGATTCA CAGGTCCTCC AGGACCACCT 
GGGATTAAAG GTGACAAAGG GATCCCAGGA ATCCCTGGAC AATCTGGCCC TCCAGGATTG 
CCTGGGGACC AAGGACCGCC AGGTGTTGCA GGACAGAAAG GAGACCCGGG AGATGCTGTC 
TCTATTGGTA GGATGAAGGG CGATAAAGGA GACACGGGCT TTCCGGGTCC ACCTGGTCTA 
CCTGGCCTGA ATGGAAGACC TGGCCAAGAC GGAATCCCAG GTTCACCTGG ACCAATAGGG 
TTACCTGGAT CTCTCTTCGT TAAAGAGGAA CATGGCCCAC CAGGGGAACC TGGAATCCCA 
GGCATTCCTG GGGACAGGGG ATCTCCAGGA GCACCAGGGT TTGGCCTTCA AGGGCCTCCA 
GGAGAGAAAG GTGTCCAAGG TGTATCGGGG CGGGCAGGTC CACCTGGTCC CCCAGGTCAA 
AAAGGTGAGC CAGGCCAGGT TGTTTCAGCG AAGGGTGTCC CTGGTCCTAT AGGGCTGAGT 
GGCGAGCCTG GCATCGCAGG ACCTCCTGGT GAGTCCGGCC AGCCTGGACA GCCTGGGTTC 
CCAGGGTTGC CTGGAGCAAA GGGAGACCTT GGACTTCCTG GCATTGGCTT ACCGGGTCCT 
CCAGGAGTCA AAGGATTCCC TGGGACACCA GGTCAGCCTG GAAGCTCTGG CAGCCCAGGA 
CCACCTGGAT TAGATGGCCT CCCTGGGCTG CCAGGCCCCC CAGGGGCCAA GGGTGACCCT 
GGGTTTGGGC TGCCAGGACC TGAAGGACCT CCTGGATTTC CTGGTAGCAA AGGAACGCAA 
GGCCCTAAAG GAGACCCTGG TTTCCCAGGG ATCCCTGGCC AGCCTGGGCG ATCAGGAGTT 
GATGGTGCTC CAGGACCCAA AGGGGATCCA GGCATTCCTG GGACCCCTGG AGCTCCTGGG 
CCCCCTGGTG CTACTTCCTT TGGGCTTCAG GGTCCTCCTG GCCCTCCAGG TCCCGCAGGT 
GCTGTAGGAT CTCCAGGGTT CCCTGGAGCG AATGGTGACA AAGGGGACCC TGGACCCCCG 
AGGCCGAACA TCCCAGGACT CCCAGGGGTT AAAGGGGAAC CGGGCTCCCC TGGACTCCCA 
GGCTCTATGG GTCCTCCTGG TTCTTCTGGT CAAACGGGTC GAGAGGGACT TCCTGGATTT 
CCTGGCCCCA AAGGAGAGAT GGGGGTGATG GGCACCGCAG GCCCTCTTGG ACCTCCAGGA 
AACTCTGGTG TTCCTGGACT CACAGGACCA AAAGGTGAGG GTGGCTCTCC CGGGCAACCT 
GGTGGACCAG GTGCTCCTGG GCCAAAGGGG GATCGAGGGG ATCCTGGTAT TCCTGGTCCC 
CCTGGCCCAA GACTTGTCGT GCAGCAGCTA CAGGGAGCAA AGGGAGAAGA GGGTCTTCCA 
GGTGCAGCCG GAGTGTCGGG ACACAAGGGT GCGCAAGGAC TCCCAGGAGA TGCTGGTCTT 
CCTGAACTTG ACGGAATACC AGGACAGCCG GGACCTCCAG GACCCAAAGG AGAGCCTGGG 
CTCCCGGGTG AACCAGGGTC CCCAGGACCA CCGGGACCAA AAGGCAATAT GGGCGAGATG 
GGATTCCCAG GTCCCAGTGG GGTGAAAGGT AACCCTGGAC AGCCAGGAAG GTCGGGCCCA 
CTTGGACAGC CAGGCACCCC TGGTTTCAGG GGAGAGAAGG GTGATCCTGG TCCTCCTGGG 
TTTGGAGTCC CAGGAAATCC AGGTACCAAG GGTGAACTGG GACAACCTGG ATTCCCTGGA 
ATCCCAGGAC CCAAAGGCCA GTCAGGTGCA CCGGGTTTAC CGGGTCTGCC TGGCAGTCCA 
GGAGAAAAGA GAAACGCAGG ACTCCCTGGA ATTCCAGGCC TTCCGGGTAT TCCTGGACCA 
AAAGGCTTCG ATGGAGCTCC AGGATCCCCA GGCGCTGATG GGCCAACAGG TCAGCCAGGA 
GAACCAGGCC GTCCTGGACC GCCTGGGCTG CCAGGAGACA AAGGCCCGCT TGGGCGCGAT 
GGCATACCTG GACCGTCTGG GGTGAAAGGA GACCCGGGTC AGCCTGGCTC GGGTCGCCTT 
GGTGTTCCAG GTCCCACAGG GTTACCAGGA CCAAAGGGAG GCGGTGGCGT CCCTGGTCTT 
CCTGGAAGCC CCGGTTTCCC TGGACGAAAG GGTGAGACGG GAACGCCCGG CATACCCGGA 
TACTCCGGAA ACGTCGGACC TCCGGGTCCC CCCGGTCCGT CACGAGCGGG GCCCAAAGGT 
AGCGCAGGGC CTCCGGGTGC CCCAGGAGGA AGCGCAGAAA ATACCTACCC GGTGGTCACC 
ATGGACAGTA TGAAAGGGCA GGCGTTAGAT CGGCAGGTCG TCTCGGCCCC GAGGGTCCAC 
GCGGCCCCCC TGGCTCGGGA GGGCTCGAGG GGCAAAAGGG CGCTGCTGGT CCCCCTGGTC 
AGCCTGGCCT GCCTGGTCAG AAGGGAGAGA GCGGAACCGC AGGCCTTCCG GGATCTCCCG 
GTCTTCCCGG GCCAGGGGGA CTCAAAGGAG ACGCCGGCCT TCCCGGACAA CCAGGGCTTC 
CAGGTCAGCG CGCGCCGCTT CGGTCTGCTC GTGCGCTCCG GTCTGTTCCT CACACTAAGA 
CGGAGACATA AATCGGAGTT TCACATGCGG GACGCCGGAG GGACCTTGAA CACTGTGCTC 
GTTGTGCTTT GCAGCCACAG CCGTGGCCTT TCGACGAAAG GAGCAGGAGG TCTGTCTGGC 
ACGCCCGGAA CACTCGGTGA CCCAGGAGAC AAAGGAGATG TCGGTCCACA AGGACCCCCG 
GGTGACCCGG GCCAGATTGC GGCCCAGATC GTGGTGAAAG GCGATCGGGG GCCCCCTGGT 
ACCCAGGGTC GCCCAGGTTC ACGTGGACCC GTGGGTCTTC CAGGTTTCGA GGGCTTGTCA 
GGTCCGCCAG GAGAACCTGG AGAACCGGGT GCTCGGGGTG CCATAGGGTT CAGCGGACCT 
TTGGGTCACA AAGGAGACGA CGGGCCACCT GGAGAGCCAG GTCAGAGGGG CTTCCCAGGC 
CCTCCCGGAC CAGATGGGCT CCGGGGACCG TCGGGCACAC CGGGGACCTC CTCGGCGGCT 
CACGGCTTTC TGGTGACGCG CCACAGCCAG TCTAGCGAGG ACCCCTTCTG CCCCGAGGGC 
ACGAGTCTCC TGTATCGTGG CTACTCCCTG CTGTACGTGC AGGGCAACGA GCGCTCCCAC 
GGTCAGGACT TGGGGACGGC GGGGAGCTGC CTGCGCAGGT TCAGCGCCAT GCCGTTCATG 
TTCTGCAACA TCAACAACGT CTGCAGCTTG GCGACGCGCA GCGACTACTC CTACTGGCTG 
TCCACACCAC AGGCCATGTC TGCGAGCATG GCGCCCGTCG CCGGGGACGA CGTCAAGCCC 
TACATCAGCA GGTGTGCGGT GTGTGAAGCT CCTGCGATGG TGGTCGCCGT GCACAGCCAG 
TCCATCCGGC TGCCCTCCTG CCCGGCCGGC TGGGACCCGC TGTGGATCGG CTACTCCTTC 
ATGATGCACA CCGGTGCGGG GGCGGAGGGT TCCGGTCAAG CCCTGGCCTC CCCCGGCTCC 
TGCCTCGAGG ACTTCCGCTC CTCCCCCTTC GTCGAATGCC ACGGAAAAGG CGCCTGCAAT 
TACTACGCCG ACTCCTACAG CTTCTGGCTG GCCACCGTGG ACGACGGCGA CATGTTTCGG 
AAGCCTCAGG AGGAGACCCT GAAAGCAGGA GACCTTGAGA CGCGGGTGAG CCGCTGTGCG 
GTGTGCATGA AGAGGACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751907.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607423.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X7, mRNA.

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CloneID	OSc54535
Clone ID Related Accession (Same CDS sequence)	XM_018751907.1
Accession Version	XM_018751907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGGAA GCCTCAGGAG GAGACCCTGA AAGCAGGAGA 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607423.1
CDS	125..5230
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQVSARRFGLLVRSGLFLTLRRRHKSEFHMRDAGGTLNTVLVVLCSHSRGLSTKGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMEASGGDPESRRP

Target ORF information:

RefSeq Version	XM_018751907.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751907.1

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGTCAGC GCGCGCCGCT TCGGTCTGCT CGTGCGCTCC 
GGTCTGTTCC TCACACTAAG ACGGAGACAT AAATCGGAGT TTCACATGCG GGACGCCGGA 
GGGACCTTGA ACACTGTGCT CGTTGTGCTT TGCAGCCACA GCCGTGGCCT TTCGACGAAA 
GGAGCAGGAG GTCTGTCTGG CACGCCCGGA ACACTCGGTG ACCCAGGAGA CAAAGGAGAT 
GTCGGTCCAC AAGGACCCCC GGGTGACCCG GGCCAGATTG CGGCCCAGAT CGTGGTGAAA 
GGCGATCGGG GGCCCCCTGG TACCCAGGGT CGCCCAGGTT CACGTGGACC CGTGGGTCTT 
CCAGGTTTCG AGGGCTTGTC AGGTCCGCCA GGAGAACCTG GAGAACCGGG TGCTCGGGGT 
GCCATAGGGT TCAGCGGACC TTTGGGTCAC AAAGGAGACG ACGGGCCACC TGGAGAGCCA 
GGTCAGAGGG GCTTCCCAGG CCCTCCCGGA CCAGATGGGC TCCGGGGACC GTCGGGCACA 
CCGGGGACCT CCTCGGCGGC TCACGGCTTT CTGGTGACGC GCCACAGCCA GTCTAGCGAG 
GACCCCTTCT GCCCCGAGGG CACGAGTCTC CTGTATCGTG GCTACTCCCT GCTGTACGTG 
CAGGGCAACG AGCGCTCCCA CGGTCAGGAC TTGGGGACGG CGGGGAGCTG CCTGCGCAGG 
TTCAGCGCCA TGCCGTTCAT GTTCTGCAAC ATCAACAACG TCTGCAGCTT GGCGACGCGC 
AGCGACTACT CCTACTGGCT GTCCACACCA CAGGCCATGT CTGCGAGCAT GGCGCCCGTC 
GCCGGGGACG ACGTCAAGCC CTACATCAGC AGGTGTGCGG TGTGTGAAGC TCCTGCGATG 
GTGGTCGCCG TGCACAGCCA GTCCATCCGG CTGCCCTCCT GCCCGGCCGG CTGGGACCCG 
CTGTGGATCG GCTACTCCTT CATGATGGAA GCCTCAGGAG GAGACCCTGA AAGCAGGAGA 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751908.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607424.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OSc54536
Clone ID Related Accession (Same CDS sequence)	XM_018751908.1
Accession Version	XM_018751908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGGTCGT 
CTCGGCCCCG AGGGTCCACG CGGCCCCCCT GGCTCGGGAG GGCTCGAGGG GCAAAAGGGC 
GCTGCTGGTC CCCCTGGTCA GCCTGGCCTG CCTGGTCAGA AGGGAGAGAG CGGAACCGCA 
GGCCTTCCGG GATCTCCCGG TCTTCCCGGG CCAGGGGGAC TCAAAGGAGA CGCCGGCCTT 
CCCGGACAAC CAGGGCTTCC AGGAGCAGGA GGTCTGTCTG GCACGCCCGG AACACTCGGT 
GACCCAGGAG ACAAAGGAGA TGTCGGTCCA CAAGGACCCC CGGGTGACCC GGGCCAGATT 
GCGGCCCAGA TCGTGGTGAA AGGCGATCGG GGGCCCCCTG GTACCCAGGG TCGCCCAGGT 
TCACGTGGAC CCGTGGGTCT TCCAGGTTTC GAGGGCTTGT CAGGTCCGCC AGGAGAACCT 
GGAGAACCGG GTGCTCGGGG TGCCATAGGG TTCAGCGGAC CTTTGGGTCA CAAAGGAGAC 
GACGGGCCAC CTGGAGAGCC AGGTCAGAGG GGCTTCCCAG GCCCTCCCGG ACCAGATGGG 
CTCCGGGGAC CGTCGGGCAC ACCGGGGACC TCCTCGGCGG CTCACGGCTT TCTGGTGACG 
CGCCACAGCC AGTCTAGCGA GGACCCCTTC TGCCCCGAGG GCACGAGTCT CCTGTATCGT 
GGCTACTCCC TGCTGTACGT GCAGGGCAAC GAGCGCTCCC ACGGTCAGGA CTTGGGGACG 
GCGGGGAGCT GCCTGCGCAG GTTCAGCGCC ATGCCGTTCA TGTTCTGCAA CATCAACAAC 
GTCTGCAGCT TGGCGACGCG CAGCGACTAC TCCTACTGGC TGTCCACACC ACAGGCCATG 
TCTGCGAGCA TGGCGCCCGT CGCCGGGGAC GACGTCAAGC CCTACATCAG CAGGTGTGCG 
GTGTGTGAAG CTCCTGCGAT GGTGGTCGCC GTGCACAGCC AGTCCATCCG GCTGCCCTCC 
TGCCCGGCCG GCTGGGACCC GCTGTGGATC GGCTACTCCT TCATGATGCA CACCGGTGCG 
GGGGCGGAGG GTTCCGGTCA AGCCCTGGCC TCCCCCGGCT CCTGCCTCGA GGACTTCCGC 
TCCTCCCCCT TCGTCGAATG CCACGGAAAA GGCGCCTGCA ATTACTACGC CGACTCCTAC 
AGCTTCTGGC TGGCCACCGT GGACGACGGC GACATGTTTC GGAAGCCTCA GGAGGAGACC 
CTGAAAGCAG GAGACCTTGA GACGCGGGTG AGCCGCTGTG CGGTGTGCAT GAAGAGGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607424.1
CDS	125..5227
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSGRLGPEGPRGPPGSGGLEGQKGAAGPPGQPGLPGQKGESGTAGLPGSPGLPGPGGLKGDAGLPGQPGLPGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751908.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751908.1

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGGTCGT 
CTCGGCCCCG AGGGTCCACG CGGCCCCCCT GGCTCGGGAG GGCTCGAGGG GCAAAAGGGC 
GCTGCTGGTC CCCCTGGTCA GCCTGGCCTG CCTGGTCAGA AGGGAGAGAG CGGAACCGCA 
GGCCTTCCGG GATCTCCCGG TCTTCCCGGG CCAGGGGGAC TCAAAGGAGA CGCCGGCCTT 
CCCGGACAAC CAGGGCTTCC AGGAGCAGGA GGTCTGTCTG GCACGCCCGG AACACTCGGT 
GACCCAGGAG ACAAAGGAGA TGTCGGTCCA CAAGGACCCC CGGGTGACCC GGGCCAGATT 
GCGGCCCAGA TCGTGGTGAA AGGCGATCGG GGGCCCCCTG GTACCCAGGG TCGCCCAGGT 
TCACGTGGAC CCGTGGGTCT TCCAGGTTTC GAGGGCTTGT CAGGTCCGCC AGGAGAACCT 
GGAGAACCGG GTGCTCGGGG TGCCATAGGG TTCAGCGGAC CTTTGGGTCA CAAAGGAGAC 
GACGGGCCAC CTGGAGAGCC AGGTCAGAGG GGCTTCCCAG GCCCTCCCGG ACCAGATGGG 
CTCCGGGGAC CGTCGGGCAC ACCGGGGACC TCCTCGGCGG CTCACGGCTT TCTGGTGACG 
CGCCACAGCC AGTCTAGCGA GGACCCCTTC TGCCCCGAGG GCACGAGTCT CCTGTATCGT 
GGCTACTCCC TGCTGTACGT GCAGGGCAAC GAGCGCTCCC ACGGTCAGGA CTTGGGGACG 
GCGGGGAGCT GCCTGCGCAG GTTCAGCGCC ATGCCGTTCA TGTTCTGCAA CATCAACAAC 
GTCTGCAGCT TGGCGACGCG CAGCGACTAC TCCTACTGGC TGTCCACACC ACAGGCCATG 
TCTGCGAGCA TGGCGCCCGT CGCCGGGGAC GACGTCAAGC CCTACATCAG CAGGTGTGCG 
GTGTGTGAAG CTCCTGCGAT GGTGGTCGCC GTGCACAGCC AGTCCATCCG GCTGCCCTCC 
TGCCCGGCCG GCTGGGACCC GCTGTGGATC GGCTACTCCT TCATGATGCA CACCGGTGCG 
GGGGCGGAGG GTTCCGGTCA AGCCCTGGCC TCCCCCGGCT CCTGCCTCGA GGACTTCCGC 
TCCTCCCCCT TCGTCGAATG CCACGGAAAA GGCGCCTGCA ATTACTACGC CGACTCCTAC 
AGCTTCTGGC TGGCCACCGT GGACGACGGC GACATGTTTC GGAAGCCTCA GGAGGAGACC 
CTGAAAGCAG GAGACCTTGA GACGCGGGTG AGCCGCTGTG CGGTGTGCAT GAAGAGGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751909.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607425.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OSc54537
Clone ID Related Accession (Same CDS sequence)	XM_018751909.1
Accession Version	XM_018751909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC GGAACACTCG 
GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC CCGGGCCAGA 
TTGCGGCCCA GATCGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607425.1
CDS	125..4345
Translation	MSCWIACKNEDFVRYKSAEPAARGAPPQREPGGASSAGQHVFWLHGRLRLCLQWRERRQGSTGPSWTSGSAGASGISWAGGAGGTQRGEGRRRAAGTGRSERNPGTVRRCLQGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSAENTYPVVTMDSMKGQALDRQVVSAPRVHAAPLAREGSRGKRALLVPLVSLACLVRRERAEPQAFRDLPVFPGQGDSKETPAFPDNQGFQEQEVCLARPEHSVTQETKEMSVHKDPRVTRARLRPRSW

Target ORF information:

RefSeq Version	XM_018751909.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751909.1

ORF Insert Sequence:

ATGAGCTGTT GGATCGCGTG CAAAAATGAA GACTTCGTGC GGTACAAGAG TGCGGAACCT 
GCTGCGCGTG GGGCTCCTCC TCAGCGCGAG CCTGGGGGTG CGAGCTCTGC AGGGCAACAT 
GTGTTTTGGC TGCACGGGAG ACTCCGCTTG TGCCTGCAGT GGCGTGAAAG GAGACAAGGG 
TCAACGGGGC CTTCCTGGAC TTCAGGGTCC GCCGGGGCTT CCGGGATTTC CTGGGCCGGA 
GGGGCAGGAG GGACCCAGAG GGGAGAAGGG CGCCGACGGG CCGCCGGGAC CGGCCGGTCC 
GAAAGGAATC CGGGTACTGT ACGACGTTGT TTGCAGGGTC CTCCCGGACA GCCTGGATTT 
CCGGGAACCC CAGGAATCCC GGGTCTACCA GGACAGGATG GCCCCCGGGG ACCCACGGGA 
CCGGCGGGGT GCAATGGGAC CAAGGGTGAA CGAGGATTGC CTGGAAACTC TGGCTTTCCT 
GGGGTACAAG GTCCCCCGGG TCCATCTGGT ATACCGGGCG CAAAGGGAGA CCCTGGTTTG 
ATCCTCTCGG CGGGTCCATT CGGACAGATG GGAGAACCGG GCTCGCCTGG ACTTCCAGGT 
GTTCCTGGCA GTCAAGGCCT TCCGGGACTA GCAGGCCCAA CAGGACCACC CGGACCCCCA 
GGCTTTCAGG GGAGGCCTGG ACCACCGGGG CCTCCAGGGC CCAAGGGTAA CATGGGCCTG 
AACTTCCAAG GAGAGAAGGG GAAGAAGGGT GAACAAGGAA TGCGTGGGCA ACCGGGGCCC 
CCCGGCCTCA TTCGTGAGCA GAAGCAAGCG AGGGATTCGG AGGTGCGGAG GGGAAACAAG 
GGGGATCAGG GACCACCTGG TCCACCTGGG AAACCTGGGG TTCCTGGGCC TCTCGGTCCT 
CCCGGTGGTC CGAAGGGTGA GAAGGGGGAG CCTGGGGAAC CAGGCAAGAG GGGGAAGCCA 
GGGAAAGATG GTGATCCTGG ACCAGTCGGT TTCCCGGGTT CGAATGGAGA GCCTGGGGGT 
CCGGGTTTGC CCGGAACGCA TGGTATTACG GGCGAGAAAG GAGACCGCGG TCTCCCAGGC 
CTCCCGGGTG CAGTGGTGAC AGGTTCGAAT CGTATCAGTG AAGGACCAAA GGGAGAGCCT 
GGTTTACCTG GACAGCTTGG GCAGAAAGGA GAGTCAGGTC CACCAGGTCT TCCGGGACTT 
CCTGGACAGC CAGGTGCTCC AGGGTTTGGC TCCCCAGGCT CACCTGGTCA GCCCGGTTTT 
CCTGGGGACA GAGGGCAGAG AGGTAATGAG GGACAGCCAG GAGTATCTTT GCCTGGACTG 
CCGGGGAGAG ACGGGCAGCC TGGGCCTCCG GGCCCCCCGG GGCCCCCAGG GCTGCCAGGA 
ACATCATCTG GAAATGGGCT CTGCCTTCGG GGGACGCCAG GGCTTCCAGG CCTTCGGGGA 
GAGCAAGGAT TTCCAGGAGA CAGAGGGCAG CCGGGTGACA AAGGGGAGAC CTGCATTAAC 
TGCTTCTCCG GTGCCGATGC CATTCCGGGG CCTCCGGGTC CTCCCGGTCC ATCCGGATTC 
ACAGGTCCTC CAGGACCACC TGGGATTAAA GGTGACAAAG GGATCCCAGG AATCCCTGGA 
CAATCTGGCC CTCCAGGATT GCCTGGGGAC CAAGGACCGC CAGGTGTTGC AGGACAGAAA 
GGAGACCCGG GAGATGCTGT CTCTATTGGT AGGATGAAGG GCGATAAAGG AGACACGGGC 
TTTCCGGGTC CACCTGGTCT ACCTGGCCTG AATGGAAGAC CTGGCCAAGA CGGAATCCCA 
GGTTCACCTG GACCAATAGG GTTACCTGGA TCTCTCTTCG TTAAAGAGGA ACATGGCCCA 
CCAGGGGAAC CTGGAATCCC AGGCATTCCT GGGGACAGGG GATCTCCAGG AGCACCAGGG 
TTTGGCCTTC AAGGGCCTCC AGGAGAGAAA GGTGTCCAAG GTGTATCGGG GCGGGCAGGT 
CCACCTGGTC CCCCAGGTCA AAAAGGTGAG CCAGGCCAGG TTGTTTCAGC GAAGGGTGTC 
CCTGGTCCTA TAGGGCTGAG TGGCGAGCCT GGCATCGCAG GACCTCCTGG TGAGTCCGGC 
CAGCCTGGAC AGCCTGGGTT CCCAGGGTTG CCTGGAGCAA AGGGAGACCT TGGACTTCCT 
GGCATTGGCT TACCGGGTCC TCCAGGAGTC AAAGGATTCC CTGGGACACC AGGTCAGCCT 
GGAAGCTCTG GCAGCCCAGG ACCACCTGGA TTAGATGGCC TCCCTGGGCT GCCAGGCCCC 
CCAGGGGCCA AGGGTGACCC TGGGTTTGGG CTGCCAGGAC CTGAAGGACC TCCTGGATTT 
CCTGGTAGCA AAGGAACGCA AGGCCCTAAA GGAGACCCTG GTTTCCCAGG GATCCCTGGC 
CAGCCTGGGC GATCAGGAGT TGATGGTGCT CCAGGACCCA AAGGGGATCC AGGCATTCCT 
GGGACCCCTG GAGCTCCTGG GCCCCCTGGT GCTACTTCCT TTGGGCTTCA GGGTCCTCCT 
GGCCCTCCAG GTCCCGCAGG TGCTGTAGGA TCTCCAGGGT TCCCTGGAGC GAATGGTGAC 
AAAGGGGACC CTGGACCCCC GAGGCCGAAC ATCCCAGGAC TCCCAGGGGT TAAAGGGGAA 
CCGGGCTCCC CTGGACTCCC AGGCTCTATG GGTCCTCCTG GTTCTTCTGG TCAAACGGGT 
CGAGAGGGAC TTCCTGGATT TCCTGGCCCC AAAGGAGAGA TGGGGGTGAT GGGCACCGCA 
GGCCCTCTTG GACCTCCAGG AAACTCTGGT GTTCCTGGAC TCACAGGACC AAAAGGTGAG 
GGTGGCTCTC CCGGGCAACC TGGTGGACCA GGTGCTCCTG GGCCAAAGGG GGATCGAGGG 
GATCCTGGTA TTCCTGGTCC CCCTGGCCCA AGACTTGTCG TGCAGCAGCT ACAGGGAGCA 
AAGGGAGAAG AGGGTCTTCC AGGTGCAGCC GGAGTGTCGG GACACAAGGG TGCGCAAGGA 
CTCCCAGGAG ATGCTGGTCT TCCTGAACTT GACGGAATAC CAGGACAGCC GGGACCTCCA 
GGACCCAAAG GAGAGCCTGG GCTCCCGGGT GAACCAGGGT CCCCAGGACC ACCGGGACCA 
AAAGGCAATA TGGGCGAGAT GGGATTCCCA GGTCCCAGTG GGGTGAAAGG TAACCCTGGA 
CAGCCAGGAA GGTCGGGCCC ACTTGGACAG CCAGGCACCC CTGGTTTCAG GGGAGAGAAG 
GGTGATCCTG GTCCTCCTGG GTTTGGAGTC CCAGGAAATC CAGGTACCAA GGGTGAACTG 
GGACAACCTG GATTCCCTGG AATCCCAGGA CCCAAAGGCC AGTCAGGTGC ACCGGGTTTA 
CCGGGTCTGC CTGGCAGTCC AGGAGAAAAG AGAAACGCAG GACTCCCTGG AATTCCAGGC 
CTTCCGGGTA TTCCTGGACC AAAAGGCTTC GATGGAGCTC CAGGATCCCC AGGCGCTGAT 
GGGCCAACAG GTCAGCCAGG AGAACCAGGC CGTCCTGGAC CGCCTGGGCT GCCAGGAGAC 
AAAGGCCCGC TTGGGCGCGA TGGCATACCT GGACCGTCTG GGGTGAAAGG AGACCCGGGT 
CAGCCTGGCT CGGGTCGCCT TGGTGTTCCA GGTCCCACAG GGTTACCAGG ACCAAAGGGA 
GGCGGTGGCG TCCCTGGTCT TCCTGGAAGC CCCGGTTTCC CTGGACGAAA GGGTGAGACG 
GGAACGCCCG GCATACCCGG ATACTCCGGA AACGTCGGAC CTCCGGGTCC CCCCGGTCCG 
TCACGAGCGG GGCCCAAAGG TAGCGCAGGG CCTCCGGGTG CCCCAGGAGG AAGCGCAGAA 
AATACCTACC CGGTGGTCAC CATGGACAGT ATGAAAGGGC AGGCGTTAGA TCGGCAGGTC 
GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG GGGCAAAAGG 
GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG AGCGGAACCG 
CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA GACGCCGGCC 
TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC GGAACACTCG 
GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC CCGGGCCAGA 
TTGCGGCCCA GATCGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108934306 ( XM_018751911.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018607427.1 Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X10, mRNA.

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CloneID	OSc54538
Clone ID Related Accession (Same CDS sequence)	XM_018751911.1
Accession Version	XM_018751911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-5(IV) chain-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGGTC GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG 
GGGCAAAAGG GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG 
AGCGGAACCG CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA 
GACGCCGGCC TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC 
GGAACACTCG GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC 
CCGGGCCAGA TTGCGGCCCA GATCGTGGTG AAAGGCGATC GGGGGCCCCC TGGTACCCAG 
GGTCGCCCAG GTTCACGTGG ACCCGTGGGT CTTCCAGGTT TCGAGGGCTT GTCAGGTCCG 
CCAGGAGAAC CTGGAGAACC GGGTGCTCGG GGTGCCATAG GGTTCAGCGG ACCTTTGGGT 
CACAAAGGAG ACGACGGGCC ACCTGGAGAG CCAGGTCAGA GGGGCTTCCC AGGCCCTCCC 
GGACCAGATG GGCTCCGGGG ACCGTCGGGC ACACCGGGGA CCTCCTCGGC GGCTCACGGC 
TTTCTGGTGA CGCGCCACAG CCAGTCTAGC GAGGACCCCT TCTGCCCCGA GGGCACGAGT 
CTCCTGTATC GTGGCTACTC CCTGCTGTAC GTGCAGGGCA ACGAGCGCTC CCACGGTCAG 
GACTTGGGGA CGGCGGGGAG CTGCCTGCGC AGGTTCAGCG CCATGCCGTT CATGTTCTGC 
AACATCAACA ACGTCTGCAG CTTGGCGACG CGCAGCGACT ACTCCTACTG GCTGTCCACA 
CCACAGGCCA TGTCTGCGAG CATGGCGCCC GTCGCCGGGG ACGACGTCAA GCCCTACATC 
AGCAGGTGTG CGGTGTGTGA AGCTCCTGCG ATGGTGGTCG CCGTGCACAG CCAGTCCATC 
CGGCTGCCCT CCTGCCCGGC CGGCTGGGAC CCGCTGTGGA TCGGCTACTC CTTCATGATG 
CACACCGGTG CGGGGGCGGA GGGTTCCGGT CAAGCCCTGG CCTCCCCCGG CTCCTGCCTC 
GAGGACTTCC GCTCCTCCCC CTTCGTCGAA TGCCACGGAA AAGGCGCCTG CAATTACTAC 
GCCGACTCCT ACAGCTTCTG GCTGGCCACC GTGGACGACG GCGACATGTT TCGGAAGCCT 
CAGGAGGAGA CCCTGAAAGC AGGAGACCTT GAGACGCGGG TGAGCCGCTG TGCGGTGTGC 
ATGAAGAGGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018607427.1
CDS	150..5204
Translation	MKTSCGTRVRNLLRVGLLLSASLGVRALQGNMCFGCTGDSACACSGVKGDKGQRGLPGLQGPPGLPGFPGPEGQEGPRGEKGADGPPGPAGPKGIRGPPGQPGFPGTPGIPGLPGQDGPRGPTGPAGCNGTKGERGLPGNSGFPGVQGPPGPSGIPGAKGDPGLILSAGPFGQMGEPGSPGLPGVPGSQGLPGLAGPTGPPGPPGFQGRPGPPGPPGPKGNMGLNFQGEKGKKGEQGMRGQPGPPGLIREQKQARDSEVRRGNKGDQGPPGPPGKPGVPGPLGPPGGPKGEKGEPGEPGKRGKPGKDGDPGPVGFPGSNGEPGGPGLPGTHGITGEKGDRGLPGLPGAVVTGSNRISEGPKGEPGLPGQLGQKGESGPPGLPGLPGQPGAPGFGSPGSPGQPGFPGDRGQRGNEGQPGVSLPGLPGRDGQPGPPGPPGPPGLPGTSSGNGLCLRGTPGLPGLRGEQGFPGDRGQPGDKGETCINCFSGADAIPGPPGPPGPSGFTGPPGPPGIKGDKGIPGIPGQSGPPGLPGDQGPPGVAGQKGDPGDAVSIGRMKGDKGDTGFPGPPGLPGLNGRPGQDGIPGSPGPIGLPGSLFVKEEHGPPGEPGIPGIPGDRGSPGAPGFGLQGPPGEKGVQGVSGRAGPPGPPGQKGEPGQVVSAKGVPGPIGLSGEPGIAGPPGESGQPGQPGFPGLPGAKGDLGLPGIGLPGPPGVKGFPGTPGQPGSSGSPGPPGLDGLPGLPGPPGAKGDPGFGLPGPEGPPGFPGSKGTQGPKGDPGFPGIPGQPGRSGVDGAPGPKGDPGIPGTPGAPGPPGATSFGLQGPPGPPGPAGAVGSPGFPGANGDKGDPGPPRPNIPGLPGVKGEPGSPGLPGSMGPPGSSGQTGREGLPGFPGPKGEMGVMGTAGPLGPPGNSGVPGLTGPKGEGGSPGQPGGPGAPGPKGDRGDPGIPGPPGPRLVVQQLQGAKGEEGLPGAAGVSGHKGAQGLPGDAGLPELDGIPGQPGPPGPKGEPGLPGEPGSPGPPGPKGNMGEMGFPGPSGVKGNPGQPGRSGPLGQPGTPGFRGEKGDPGPPGFGVPGNPGTKGELGQPGFPGIPGPKGQSGAPGLPGLPGSPGEKRNAGLPGIPGLPGIPGPKGFDGAPGSPGADGPTGQPGEPGRPGPPGLPGDKGPLGRDGIPGPSGVKGDPGQPGSGRLGVPGPTGLPGPKGGGGVPGLPGSPGFPGRKGETGTPGIPGYSGNVGPPGPPGPSRAGPKGSAGPPGAPGGSGRLGPEGPRGPPGSGGLEGQKGAAGPPGQPGLPGQKGESGTAGLPGSPGLPGPGGLKGDAGLPGQPGLPGAGGLSGTPGTLGDPGDKGDVGPQGPPGDPGQIAAQIVVKGDRGPPGTQGRPGSRGPVGLPGFEGLSGPPGEPGEPGARGAIGFSGPLGHKGDDGPPGEPGQRGFPGPPGPDGLRGPSGTPGTSSAAHGFLVTRHSQSSEDPFCPEGTSLLYRGYSLLYVQGNERSHGQDLGTAGSCLRRFSAMPFMFCNINNVCSLATRSDYSYWLSTPQAMSASMAPVAGDDVKPYISRCAVCEAPAMVVAVHSQSIRLPSCPAGWDPLWIGYSFMMHTGAGAEGSGQALASPGSCLEDFRSSPFVECHGKGACNYYADSYSFWLATVDDGDMFRKPQEETLKAGDLETRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_018751911.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen alpha-5(IV) chain-like (LOC108934306), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018751911.1

ORF Insert Sequence:

ATGAAGACTT CGTGCGGTAC AAGAGTGCGG AACCTGCTGC GCGTGGGGCT CCTCCTCAGC 
GCGAGCCTGG GGGTGCGAGC TCTGCAGGGC AACATGTGTT TTGGCTGCAC GGGAGACTCC 
GCTTGTGCCT GCAGTGGCGT GAAAGGAGAC AAGGGTCAAC GGGGCCTTCC TGGACTTCAG 
GGTCCGCCGG GGCTTCCGGG ATTTCCTGGG CCGGAGGGGC AGGAGGGACC CAGAGGGGAG 
AAGGGCGCCG ACGGGCCGCC GGGACCGGCC GGTCCGAAAG GAATCCGGGG TCCTCCCGGA 
CAGCCTGGAT TTCCGGGAAC CCCAGGAATC CCGGGTCTAC CAGGACAGGA TGGCCCCCGG 
GGACCCACGG GACCGGCGGG GTGCAATGGG ACCAAGGGTG AACGAGGATT GCCTGGAAAC 
TCTGGCTTTC CTGGGGTACA AGGTCCCCCG GGTCCATCTG GTATACCGGG CGCAAAGGGA 
GACCCTGGTT TGATCCTCTC GGCGGGTCCA TTCGGACAGA TGGGAGAACC GGGCTCGCCT 
GGACTTCCAG GTGTTCCTGG CAGTCAAGGC CTTCCGGGAC TAGCAGGCCC AACAGGACCA 
CCCGGACCCC CAGGCTTTCA GGGGAGGCCT GGACCACCGG GGCCTCCAGG GCCCAAGGGT 
AACATGGGCC TGAACTTCCA AGGAGAGAAG GGGAAGAAGG GTGAACAAGG AATGCGTGGG 
CAACCGGGGC CCCCCGGCCT CATTCGTGAG CAGAAGCAAG CGAGGGATTC GGAGGTGCGG 
AGGGGAAACA AGGGGGATCA GGGACCACCT GGTCCACCTG GGAAACCTGG GGTTCCTGGG 
CCTCTCGGTC CTCCCGGTGG TCCGAAGGGT GAGAAGGGGG AGCCTGGGGA ACCAGGCAAG 
AGGGGGAAGC CAGGGAAAGA TGGTGATCCT GGACCAGTCG GTTTCCCGGG TTCGAATGGA 
GAGCCTGGGG GTCCGGGTTT GCCCGGAACG CATGGTATTA CGGGCGAGAA AGGAGACCGC 
GGTCTCCCAG GCCTCCCGGG TGCAGTGGTG ACAGGTTCGA ATCGTATCAG TGAAGGACCA 
AAGGGAGAGC CTGGTTTACC TGGACAGCTT GGGCAGAAAG GAGAGTCAGG TCCACCAGGT 
CTTCCGGGAC TTCCTGGACA GCCAGGTGCT CCAGGGTTTG GCTCCCCAGG CTCACCTGGT 
CAGCCCGGTT TTCCTGGGGA CAGAGGGCAG AGAGGTAATG AGGGACAGCC AGGAGTATCT 
TTGCCTGGAC TGCCGGGGAG AGACGGGCAG CCTGGGCCTC CGGGCCCCCC GGGGCCCCCA 
GGGCTGCCAG GAACATCATC TGGAAATGGG CTCTGCCTTC GGGGGACGCC AGGGCTTCCA 
GGCCTTCGGG GAGAGCAAGG ATTTCCAGGA GACAGAGGGC AGCCGGGTGA CAAAGGGGAG 
ACCTGCATTA ACTGCTTCTC CGGTGCCGAT GCCATTCCGG GGCCTCCGGG TCCTCCCGGT 
CCATCCGGAT TCACAGGTCC TCCAGGACCA CCTGGGATTA AAGGTGACAA AGGGATCCCA 
GGAATCCCTG GACAATCTGG CCCTCCAGGA TTGCCTGGGG ACCAAGGACC GCCAGGTGTT 
GCAGGACAGA AAGGAGACCC GGGAGATGCT GTCTCTATTG GTAGGATGAA GGGCGATAAA 
GGAGACACGG GCTTTCCGGG TCCACCTGGT CTACCTGGCC TGAATGGAAG ACCTGGCCAA 
GACGGAATCC CAGGTTCACC TGGACCAATA GGGTTACCTG GATCTCTCTT CGTTAAAGAG 
GAACATGGCC CACCAGGGGA ACCTGGAATC CCAGGCATTC CTGGGGACAG GGGATCTCCA 
GGAGCACCAG GGTTTGGCCT TCAAGGGCCT CCAGGAGAGA AAGGTGTCCA AGGTGTATCG 
GGGCGGGCAG GTCCACCTGG TCCCCCAGGT CAAAAAGGTG AGCCAGGCCA GGTTGTTTCA 
GCGAAGGGTG TCCCTGGTCC TATAGGGCTG AGTGGCGAGC CTGGCATCGC AGGACCTCCT 
GGTGAGTCCG GCCAGCCTGG ACAGCCTGGG TTCCCAGGGT TGCCTGGAGC AAAGGGAGAC 
CTTGGACTTC CTGGCATTGG CTTACCGGGT CCTCCAGGAG TCAAAGGATT CCCTGGGACA 
CCAGGTCAGC CTGGAAGCTC TGGCAGCCCA GGACCACCTG GATTAGATGG CCTCCCTGGG 
CTGCCAGGCC CCCCAGGGGC CAAGGGTGAC CCTGGGTTTG GGCTGCCAGG ACCTGAAGGA 
CCTCCTGGAT TTCCTGGTAG CAAAGGAACG CAAGGCCCTA AAGGAGACCC TGGTTTCCCA 
GGGATCCCTG GCCAGCCTGG GCGATCAGGA GTTGATGGTG CTCCAGGACC CAAAGGGGAT 
CCAGGCATTC CTGGGACCCC TGGAGCTCCT GGGCCCCCTG GTGCTACTTC CTTTGGGCTT 
CAGGGTCCTC CTGGCCCTCC AGGTCCCGCA GGTGCTGTAG GATCTCCAGG GTTCCCTGGA 
GCGAATGGTG ACAAAGGGGA CCCTGGACCC CCGAGGCCGA ACATCCCAGG ACTCCCAGGG 
GTTAAAGGGG AACCGGGCTC CCCTGGACTC CCAGGCTCTA TGGGTCCTCC TGGTTCTTCT 
GGTCAAACGG GTCGAGAGGG ACTTCCTGGA TTTCCTGGCC CCAAAGGAGA GATGGGGGTG 
ATGGGCACCG CAGGCCCTCT TGGACCTCCA GGAAACTCTG GTGTTCCTGG ACTCACAGGA 
CCAAAAGGTG AGGGTGGCTC TCCCGGGCAA CCTGGTGGAC CAGGTGCTCC TGGGCCAAAG 
GGGGATCGAG GGGATCCTGG TATTCCTGGT CCCCCTGGCC CAAGACTTGT CGTGCAGCAG 
CTACAGGGAG CAAAGGGAGA AGAGGGTCTT CCAGGTGCAG CCGGAGTGTC GGGACACAAG 
GGTGCGCAAG GACTCCCAGG AGATGCTGGT CTTCCTGAAC TTGACGGAAT ACCAGGACAG 
CCGGGACCTC CAGGACCCAA AGGAGAGCCT GGGCTCCCGG GTGAACCAGG GTCCCCAGGA 
CCACCGGGAC CAAAAGGCAA TATGGGCGAG ATGGGATTCC CAGGTCCCAG TGGGGTGAAA 
GGTAACCCTG GACAGCCAGG AAGGTCGGGC CCACTTGGAC AGCCAGGCAC CCCTGGTTTC 
AGGGGAGAGA AGGGTGATCC TGGTCCTCCT GGGTTTGGAG TCCCAGGAAA TCCAGGTACC 
AAGGGTGAAC TGGGACAACC TGGATTCCCT GGAATCCCAG GACCCAAAGG CCAGTCAGGT 
GCACCGGGTT TACCGGGTCT GCCTGGCAGT CCAGGAGAAA AGAGAAACGC AGGACTCCCT 
GGAATTCCAG GCCTTCCGGG TATTCCTGGA CCAAAAGGCT TCGATGGAGC TCCAGGATCC 
CCAGGCGCTG ATGGGCCAAC AGGTCAGCCA GGAGAACCAG GCCGTCCTGG ACCGCCTGGG 
CTGCCAGGAG ACAAAGGCCC GCTTGGGCGC GATGGCATAC CTGGACCGTC TGGGGTGAAA 
GGAGACCCGG GTCAGCCTGG CTCGGGTCGC CTTGGTGTTC CAGGTCCCAC AGGGTTACCA 
GGACCAAAGG GAGGCGGTGG CGTCCCTGGT CTTCCTGGAA GCCCCGGTTT CCCTGGACGA 
AAGGGTGAGA CGGGAACGCC CGGCATACCC GGATACTCCG GAAACGTCGG ACCTCCGGGT 
CCCCCCGGTC CGTCACGAGC GGGGCCCAAA GGTAGCGCAG GGCCTCCGGG TGCCCCAGGA 
GGAAGCGGTC GTCTCGGCCC CGAGGGTCCA CGCGGCCCCC CTGGCTCGGG AGGGCTCGAG 
GGGCAAAAGG GCGCTGCTGG TCCCCCTGGT CAGCCTGGCC TGCCTGGTCA GAAGGGAGAG 
AGCGGAACCG CAGGCCTTCC GGGATCTCCC GGTCTTCCCG GGCCAGGGGG ACTCAAAGGA 
GACGCCGGCC TTCCCGGACA ACCAGGGCTT CCAGGAGCAG GAGGTCTGTC TGGCACGCCC 
GGAACACTCG GTGACCCAGG AGACAAAGGA GATGTCGGTC CACAAGGACC CCCGGGTGAC 
CCGGGCCAGA TTGCGGCCCA GATCGTGGTG AAAGGCGATC GGGGGCCCCC TGGTACCCAG 
GGTCGCCCAG GTTCACGTGG ACCCGTGGGT CTTCCAGGTT TCGAGGGCTT GTCAGGTCCG 
CCAGGAGAAC CTGGAGAACC GGGTGCTCGG GGTGCCATAG GGTTCAGCGG ACCTTTGGGT 
CACAAAGGAG ACGACGGGCC ACCTGGAGAG CCAGGTCAGA GGGGCTTCCC AGGCCCTCCC 
GGACCAGATG GGCTCCGGGG ACCGTCGGGC ACACCGGGGA CCTCCTCGGC GGCTCACGGC 
TTTCTGGTGA CGCGCCACAG CCAGTCTAGC GAGGACCCCT TCTGCCCCGA GGGCACGAGT 
CTCCTGTATC GTGGCTACTC CCTGCTGTAC GTGCAGGGCA ACGAGCGCTC CCACGGTCAG 
GACTTGGGGA CGGCGGGGAG CTGCCTGCGC AGGTTCAGCG CCATGCCGTT CATGTTCTGC 
AACATCAACA ACGTCTGCAG CTTGGCGACG CGCAGCGACT ACTCCTACTG GCTGTCCACA 
CCACAGGCCA TGTCTGCGAG CATGGCGCCC GTCGCCGGGG ACGACGTCAA GCCCTACATC 
AGCAGGTGTG CGGTGTGTGA AGCTCCTGCG ATGGTGGTCG CCGTGCACAG CCAGTCCATC 
CGGCTGCCCT CCTGCCCGGC CGGCTGGGAC CCGCTGTGGA TCGGCTACTC CTTCATGATG 
CACACCGGTG CGGGGGCGGA GGGTTCCGGT CAAGCCCTGG CCTCCCCCGG CTCCTGCCTC 
GAGGACTTCC GCTCCTCCCC CTTCGTCGAA TGCCACGGAA AAGGCGCCTG CAATTACTAC 
GCCGACTCCT ACAGCTTCTG GCTGGCCACC GTGGACGACG GCGACATGTT TCGGAAGCCT 
CAGGAGGAGA CCCTGAAAGC AGGAGACCTT GAGACGCGGG TGAGCCGCTG TGCGGTGTGC 
ATGAAGAGGA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC108934306 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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