THO2 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	THO2
Entrez Gene ID	3361454
Full Name	THO complex subunit Tho2 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355985.1	NP_001342811.1	putative THO complex subunit Tho2
XM_001712991.2	XP_001713043.1	THO complex subunit Tho2 (predicted)

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	tho2	XP_001713043.1
Magnaporthe oryzae (rice blast fungus)	MGG_01468	XP_003714425.1
Neurospora crassa	NCU07002	XP_962642.1
Mus musculus (house mouse)	BC005561	NP_001160053.1
Rattus norvegicus (Norway rat)	LOC679894	XP_001054874.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101759	NM_001355985.1 Latest version!	Schizosaccharomyces pombe putative THO complex subunit Tho2 (tho2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OSj04907	XM_001712991.2 Latest version!	Schizosaccharomyces pombe 972h- THO complex subunit Tho2 (predicted) (tho2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THO2 ( NM_001355985.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342811.1 Schizosaccharomyces pombe putative THO complex subunit Tho2 (tho2), mRNA.

Price & Availability↑

CloneID	OSc101759
Clone ID Related Accession (Same CDS sequence)	NM_001355985.1
Accession Version	NM_001355985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative THO complex subunit Tho2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGACTTCGT TGCCAGAAAA GGATCAGCAA GGCGAAGTTA GTGTTTCAGA AAATCAAAAG 
AAGCTCTCAG AGGAATGGGA GCCTTATATT GATAAACTTG TTGCATCTAA TAGAACGCGT 
GACGAACGTG TTGAATCAAT CCAAGAATTG TTTCAAAAAT GTGTCATTGA AGAGAAGTTA 
CCAGTAGACA TATTTTTCTC TATTTTTTCC TTAGCTTTTG AAAGATTGGA GGAAAAAAAT 
ACCTTAGCTT CTTATGTAAT TGATACGCTT TGGTTATTTG ACACAGAGTG GATCAAAAAC 
TTTCACGAAG GAAGTCATGA TAAAGCTGAA AAGAGGCTTG TTTCAATTGG AAAAGGACTA 
AAGGAATTCC TTCCAGAGGA ATGGTTGCTT TCTCGCTTGG ATTGCAAATT TTTGGAAAAT 
ATTAATGTCG TTCCTAATGG CGATTTTCTA AATCGGAAAA TTGTGCGGAC AAATACTTCG 
CTACTCTACC GCCAAAAAAA ATTTAATTTG CTTAGAGAAG AAAGTGAAGG ATTTTCACAT 
TTAATGATAA ACTTTTTTGA TGCTTTAACT TGTCTTCGCA ATAAATCCTT AAACGAAGAT 
TATCTGATCG TTCAAGTAAA TAAGTCAATT ATCAGTACTA TTGGTGCTTT TGATTTAGAT 
CCGAACAAAG TACTGGATTT GATTTTGTTG CTTTTTTCTG AAAATTTACT TGATTCGTGG 
CGCTTTTTCC TTTCTATCTT GAGAAACAGC CCATGGGGTC CGAATAAAGA GCGGAAATTT 
TGGAATCAGT TACCTGATCG CGAAAAGGAA ACATTTTTAA ATAATCTGTC TAATGCTAAT 
GGTATTTTTA ATTTTGACGA GCGATTCACC AATACAAAGA GCATAATGTC TCAAGTTTTG 
GGTCATAATT TGCAATATAT GTATAAAGAA GATGACGAGA ATTTGGAATC TTACTTTATG 
ATGGTTGCTT TGTTGATTAA ATATAATTTT ATTAGCATTG ATAACATATG GGCTCATCTT 
TCTCCATCAG ATGAAGAGCT TGGTAAAGAG TTGGGTAAAT ATAAGGATAA ATTAGATGAA 
CAGACTTTTA AGGCTAAAGG AAATGCTTTA ACAATGGCGG CCCCATTGCC CGACGATGAA 
ATTGAAGACG GAGAAACCAT GGATGGCCAG AAAGCTGAAG CTGTTCCTGA AATAAAAAAA 
GCCAAACCTT CACAGAAGCT GGGTCTACTA AAATCTTTAT TGTCCATCGG GGATTTATCT 
TCATCTCTAT TAATCTTGGG TCGTTATCCA TTTTTGTTGA GGGCGTACCC CGAATTATCT 
AACTTGTATC ACAAACTTTT GCATATTTCT ATTTCCTCAA TTTATGCAAA TTACAGTCCA 
TTGAAATTGT TACCAAATGA TGTAAGGGAA CGACTTAAAC AGCCAAAGTT CATTCCTGAA 
GACTCTAGGC TTCGAGAAAT TACCCTGAGG CCTCCAAAGG AAAAAAATTT AGTGTTTTCG 
TTGGATCCTT TTGCTGATCG ATTCAACAAA ACTGAAAGTG AGGTGTTTTA TTACTTTGAA 
AATTATGATG AAGATATACC AATTTTACGT AACTTGACAG AGTTCTACAA CATAGCTATC 
CCATGGTTAC GTTTGTCAGG GCTTGCTCTG TGTCATGATC CTGTTATAGT GACAAAGCTT 
TGCCGCATTG GTCAAAAATG TGTCGATAAT AGCTCTGAGA GTCGGACATT ATGGCTGGAT 
ATTATTAGAT CGCTTCTTCT TCCTCTCATC ACACTTATTG ATGTCAATAC TGGACTTTCC 
TATGAATTAT TTGAGTTGCT GTCAAAGTTT GATTCATCCA CTCGATATGC GTTGTATGGT 
GAATGGTCAA GTACCTCCAT GAAAAAGTTT CCGGAGCTAA AGCTACAAAA TTCTATCACT 
GAAAAGGAAA CAAAAGGCAT CTTAAGAAGG TTAACGAAAA CAAATGTGAA GCAATTTGGT 
AGATTATTGG CTAAAGTCTG TCATTCGAAT CCATGTACTG TGTTTTCGAT CGCTTTAAAT 
CAGATTGAAA CTTATGATAA TTTAGTTGAA GTTGTAGTTG ATAGTGCTCG TTTTATTACA 
GCATTAGACT TTGATGCATT GACATTTATT ATTTTATCTT CCTTTTCCAA TGAGTTCAAA 
AAGCGATTGA AATCTGATGG AACTAGTATA GCTCATTGGC TCCAAGGTTT AGCATCTTTT 
TGTGGCCGTG TATTCAGAAG ATACTCGAGC CTCGATTGCA CCTCTATCGT CGAATATGTG 
ATTAAACAGT TTAAAGTGAA CCAAATGTTT GACCTTGTTA TATTGAAAGA ATTGCTTTCG 
CAGATGACTG GGCTACAACC ATGGACAAAT CTCTCTGACA ATCAGATTCA AGGGGCTGCT 
GGTGGACCAG TTTTGCGACA ACTTAGTTTA TCTCTTATAT ATGAAAATCC GGACGTTGTT 
CGGAAAAGTT CCATGAGACT TTTTAATACG CTGCAAAAGA ACGGATTGGC AACCCAGCTA 
TTGGTACTAC TCTCTCAAAA ATATTCGACC TGTATATATG ATGTTACTGA TGAAAACTCT 
CATTTAAAAC TTATTTCTTC TCTTCAAGAC GAATGCTCTG ATGTTCTTTA TCTTTTAATG 
GAATTTCTTA ATATGGTTTG CTCTCCAAAG AGCTATTATA AATTGATTCC TTCATTTGAG 
CAATTAATTC AAGATTTTCA TATTCAACCT CAGGTTGCAT TTTATCTATC GCGTTACAAA 
AATTTAGATC ACAGTTTGAC TGGTTCAAAT ACTGAGGATG CAATGGATAT TGATTATGAA 
AATACTTCAT CTCCAAACAC TGCTAGCAAC CCCGTTTGGA GTATTGACAA TTCTGTCATA 
ACTGAGTTAT TACCTAAACA AATTTGGGAT TATTTTTCAC CTAATTTTTA TTTAACATTT 
TGGAAGCTAT CGCTGTATGA TGTTTTTGTT CCCTTGGAAC GTTATGAGTT TGAGCGTTCT 
CGGGCTTTTG ATCAAATTAG GCAAACTGAC GCCGCTAACA CATTTTATTC ACGACATCGA 
CACGACCGAC AAAAAATAAT GCAATTGTCT AACAGCTTAC AGAATGAACT TAAAGAACAC 
ATTAATTCAC TTGAGAGTGT AAGAAAAGTT TTGCAAGGTG ACTGCGTGAA ATGGTTTATT 
CCAAATGGTG TTTTTCCTAA TGGTACAAGA CTTGAGCATG CTAGATTCAA CTGTGCTCGG 
TATTTGTGGA CACTCTGCAT TGCTCCCCGT TTAAAAATGA GCCCACATGA TGCTTTGTAC 
TGCGCAAAGT TTGTAAAGCT TTTGCACAGT CTTGGAACAC CAAACTTTTC AACTATGTCA 
TTTTTAGAAA TTTTGTTTAA TTCACAATTG CCTTCATTTA TTTTTTCAAT GACACAACGA 
GAGGCTGATA ATTTTGGAAG ATTTTTATAC GAAGTACTCT ATGATATTAC TAGCTGGTAC 
AGGGACAAAA TTCTTTACGA ACGCGAATGT TTGGCAAATG GTGCTTTGCC TGGGTTCAGG 
TTATATTGGT CTGACGAACA AAATGATCCC GATCTTTCCG CTGTCCTTCC TTATAACAAA 
TTTGTTTTGC TATTTAGTAA ATGGCACAAA TATCTAACAT CTTATTTTGA GTCATGCTTA 
CTATCTACTG AATATATGCA TATCTACAAT AGTGTCATAA TCTTGGAAAA AATTTTGCCA 
TGTTTCCCAT TGATAATTGA AAGTGGCAGT GCTTTGAAAC GAGCCGCTGA ACGATTAAAG 
GACGAAGAAA AACGAGAAGA TTTAAAGGTT TTGGCTTTGG GGTATTTTGC AAAGCTCTCA 
AAAAAGCAAC CAGAATGGGT TTCTTTTAAT TCATTTTCTG GTACTGTAAG GCCGAGTAAT 
TCAGAAAAGC TTCAGAGACC GCAACAACTT TCAGTGGCAG CTACATCAGC AGTTGATTCC 
AAAACTGCTT CCATATCGGA AGAACAGGCA AAGATTGATA AACAGAAAGT AGCTTTGAAT 
CCTAGTGCAC CAGAGTTTGT ACCTGATAGT ACGCCTTCCG ACGCAGTTGC TTCTGAAACT 
GACAATAAAA ATCTTGTTGA GAACAAGGCA GTAGAGAAAA GGGTTGAAGC TAGAAGCTCA 
GCTAATGAAA GAAAACAAGA AGAAAGACGT AGAAAAACAA CTCCTGAAGG AAACAGACGT 
GCTTTACGAA CACGCACCCC TACGAATGAG GATATACAAA GATCAGACAG TAAATTGCGA 
GAGGATCAGT CTAGAGACCG AACACCTCAA TCAAGAAGTT TTACAAATGA AAACAACGAT 
AATTTAAGGA GCGTGTCTCG TCATACTCGA CGCGAGCCTC AGCAAGCACA AAACCTAAAT 
GCTAGGCGTG AACATGAATC ACAGAAATCT GACAGATGGC GCCAAAATGG AAACGTAAAT 
CGCAACCCTC GAGTGTCTAA TAACAATAGC ACTAATGTTT CAAGAGAGCG CTCGTCTGAA 
GCTAATCATA GGACAAGCAA CGATAATAAA CGTGATGAGG TTACAGAAGG GAAAGATAAA 
AACAAAAGGC AAGATATTTC TGGCGAGAGT AATTCTCGGC AGAATAATGC TATTAGTCGG 
GCTGGAAGAA GTAATGGATC TAATAGGGGA AATGATTCCA GAGATGCAGA TGGTCGTCGT 
AGCACTCATT ATGCTTCAAA TAAACGTCCC CGATCTTCGG ATAGTCAAAG CCCGAGCAAC 
CTACGAGAGG AAGATGAAAG AGAAAATTCT CGTCGTAGGG CCAGGCAAGA CGACCGAAGG 
GATCGGGATT CACGACAGCA AAGAGATCGT CCACGGGATA GAACATCTCG ATCTGCTCGA 
GAAGAGAAAA GAAGAAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342811.1
CDS	177..5063
Translation	MTSLPEKDQQGEVSVSENQKKLSEEWEPYIDKLVASNRTRDERVESIQELFQKCVIEEKLPVDIFFSIFSLAFERLEEKNTLASYVIDTLWLFDTEWIKNFHEGSHDKAEKRLVSIGKGLKEFLPEEWLLSRLDCKFLENINVVPNGDFLNRKIVRTNTSLLYRQKKFNLLREESEGFSHLMINFFDALTCLRNKSLNEDYLIVQVNKSIISTIGAFDLDPNKVLDLILLLFSENLLDSWRFFLSILRNSPWGPNKERKFWNQLPDREKETFLNNLSNANGIFNFDERFTNTKSIMSQVLGHNLQYMYKEDDENLESYFMMVALLIKYNFISIDNIWAHLSPSDEELGKELGKYKDKLDEQTFKAKGNALTMAAPLPDDEIEDGETMDGQKAEAVPEIKKAKPSQKLGLLKSLLSIGDLSSSLLILGRYPFLLRAYPELSNLYHKLLHISISSIYANYSPLKLLPNDVRERLKQPKFIPEDSRLREITLRPPKEKNLVFSLDPFADRFNKTESEVFYYFENYDEDIPILRNLTEFYNIAIPWLRLSGLALCHDPVIVTKLCRIGQKCVDNSSESRTLWLDIIRSLLLPLITLIDVNTGLSYELFELLSKFDSSTRYALYGEWSSTSMKKFPELKLQNSITEKETKGILRRLTKTNVKQFGRLLAKVCHSNPCTVFSIALNQIETYDNLVEVVVDSARFITALDFDALTFIILSSFSNEFKKRLKSDGTSIAHWLQGLASFCGRVFRRYSSLDCTSIVEYVIKQFKVNQMFDLVILKELLSQMTGLQPWTNLSDNQIQGAAGGPVLRQLSLSLIYENPDVVRKSSMRLFNTLQKNGLATQLLVLLSQKYSTCIYDVTDENSHLKLISSLQDECSDVLYLLMEFLNMVCSPKSYYKLIPSFEQLIQDFHIQPQVAFYLSRYKNLDHSLTGSNTEDAMDIDYENTSSPNTASNPVWSIDNSVITELLPKQIWDYFSPNFYLTFWKLSLYDVFVPLERYEFERSRAFDQIRQTDAANTFYSRHRHDRQKIMQLSNSLQNELKEHINSLESVRKVLQGDCVKWFIPNGVFPNGTRLEHARFNCARYLWTLCIAPRLKMSPHDALYCAKFVKLLHSLGTPNFSTMSFLEILFNSQLPSFIFSMTQREADNFGRFLYEVLYDITSWYRDKILYERECLANGALPGFRLYWSDEQNDPDLSAVLPYNKFVLLFSKWHKYLTSYFESCLLSTEYMHIYNSVIILEKILPCFPLIIESGSALKRAAERLKDEEKREDLKVLALGYFAKLSKKQPEWVSFNSFSGTVRPSNSEKLQRPQQLSVAATSAVDSKTASISEEQAKIDKQKVALNPSAPEFVPDSTPSDAVASETDNKNLVENKAVEKRVEARSSANERKQEERRRKTTPEGNRRALRTRTPTNEDIQRSDSKLREDQSRDRTPQSRSFTNENNDNLRSVSRHTRREPQQAQNLNARREHESQKSDRWRQNGNVNRNPRVSNNNSTNVSRERSSEANHRTSNDNKRDEVTEGKDKNKRQDISGESNSRQNNAISRAGRSNGSNRGNDSRDADGRRSTHYASNKRPRSSDSQSPSNLREEDERENSRRRARQDDRRDRDSRQQRDRPRDRTSRSAREEKRRKIQ

Target ORF information:

RefSeq Version	NM_001355985.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative THO complex subunit Tho2 (tho2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355985.1

ORF Insert Sequence:

ATGACTTCGT TGCCAGAAAA GGATCAGCAA GGCGAAGTTA GTGTTTCAGA AAATCAAAAG 
AAGCTCTCAG AGGAATGGGA GCCTTATATT GATAAACTTG TTGCATCTAA TAGAACGCGT 
GACGAACGTG TTGAATCAAT CCAAGAATTG TTTCAAAAAT GTGTCATTGA AGAGAAGTTA 
CCAGTAGACA TATTTTTCTC TATTTTTTCC TTAGCTTTTG AAAGATTGGA GGAAAAAAAT 
ACCTTAGCTT CTTATGTAAT TGATACGCTT TGGTTATTTG ACACAGAGTG GATCAAAAAC 
TTTCACGAAG GAAGTCATGA TAAAGCTGAA AAGAGGCTTG TTTCAATTGG AAAAGGACTA 
AAGGAATTCC TTCCAGAGGA ATGGTTGCTT TCTCGCTTGG ATTGCAAATT TTTGGAAAAT 
ATTAATGTCG TTCCTAATGG CGATTTTCTA AATCGGAAAA TTGTGCGGAC AAATACTTCG 
CTACTCTACC GCCAAAAAAA ATTTAATTTG CTTAGAGAAG AAAGTGAAGG ATTTTCACAT 
TTAATGATAA ACTTTTTTGA TGCTTTAACT TGTCTTCGCA ATAAATCCTT AAACGAAGAT 
TATCTGATCG TTCAAGTAAA TAAGTCAATT ATCAGTACTA TTGGTGCTTT TGATTTAGAT 
CCGAACAAAG TACTGGATTT GATTTTGTTG CTTTTTTCTG AAAATTTACT TGATTCGTGG 
CGCTTTTTCC TTTCTATCTT GAGAAACAGC CCATGGGGTC CGAATAAAGA GCGGAAATTT 
TGGAATCAGT TACCTGATCG CGAAAAGGAA ACATTTTTAA ATAATCTGTC TAATGCTAAT 
GGTATTTTTA ATTTTGACGA GCGATTCACC AATACAAAGA GCATAATGTC TCAAGTTTTG 
GGTCATAATT TGCAATATAT GTATAAAGAA GATGACGAGA ATTTGGAATC TTACTTTATG 
ATGGTTGCTT TGTTGATTAA ATATAATTTT ATTAGCATTG ATAACATATG GGCTCATCTT 
TCTCCATCAG ATGAAGAGCT TGGTAAAGAG TTGGGTAAAT ATAAGGATAA ATTAGATGAA 
CAGACTTTTA AGGCTAAAGG AAATGCTTTA ACAATGGCGG CCCCATTGCC CGACGATGAA 
ATTGAAGACG GAGAAACCAT GGATGGCCAG AAAGCTGAAG CTGTTCCTGA AATAAAAAAA 
GCCAAACCTT CACAGAAGCT GGGTCTACTA AAATCTTTAT TGTCCATCGG GGATTTATCT 
TCATCTCTAT TAATCTTGGG TCGTTATCCA TTTTTGTTGA GGGCGTACCC CGAATTATCT 
AACTTGTATC ACAAACTTTT GCATATTTCT ATTTCCTCAA TTTATGCAAA TTACAGTCCA 
TTGAAATTGT TACCAAATGA TGTAAGGGAA CGACTTAAAC AGCCAAAGTT CATTCCTGAA 
GACTCTAGGC TTCGAGAAAT TACCCTGAGG CCTCCAAAGG AAAAAAATTT AGTGTTTTCG 
TTGGATCCTT TTGCTGATCG ATTCAACAAA ACTGAAAGTG AGGTGTTTTA TTACTTTGAA 
AATTATGATG AAGATATACC AATTTTACGT AACTTGACAG AGTTCTACAA CATAGCTATC 
CCATGGTTAC GTTTGTCAGG GCTTGCTCTG TGTCATGATC CTGTTATAGT GACAAAGCTT 
TGCCGCATTG GTCAAAAATG TGTCGATAAT AGCTCTGAGA GTCGGACATT ATGGCTGGAT 
ATTATTAGAT CGCTTCTTCT TCCTCTCATC ACACTTATTG ATGTCAATAC TGGACTTTCC 
TATGAATTAT TTGAGTTGCT GTCAAAGTTT GATTCATCCA CTCGATATGC GTTGTATGGT 
GAATGGTCAA GTACCTCCAT GAAAAAGTTT CCGGAGCTAA AGCTACAAAA TTCTATCACT 
GAAAAGGAAA CAAAAGGCAT CTTAAGAAGG TTAACGAAAA CAAATGTGAA GCAATTTGGT 
AGATTATTGG CTAAAGTCTG TCATTCGAAT CCATGTACTG TGTTTTCGAT CGCTTTAAAT 
CAGATTGAAA CTTATGATAA TTTAGTTGAA GTTGTAGTTG ATAGTGCTCG TTTTATTACA 
GCATTAGACT TTGATGCATT GACATTTATT ATTTTATCTT CCTTTTCCAA TGAGTTCAAA 
AAGCGATTGA AATCTGATGG AACTAGTATA GCTCATTGGC TCCAAGGTTT AGCATCTTTT 
TGTGGCCGTG TATTCAGAAG ATACTCGAGC CTCGATTGCA CCTCTATCGT CGAATATGTG 
ATTAAACAGT TTAAAGTGAA CCAAATGTTT GACCTTGTTA TATTGAAAGA ATTGCTTTCG 
CAGATGACTG GGCTACAACC ATGGACAAAT CTCTCTGACA ATCAGATTCA AGGGGCTGCT 
GGTGGACCAG TTTTGCGACA ACTTAGTTTA TCTCTTATAT ATGAAAATCC GGACGTTGTT 
CGGAAAAGTT CCATGAGACT TTTTAATACG CTGCAAAAGA ACGGATTGGC AACCCAGCTA 
TTGGTACTAC TCTCTCAAAA ATATTCGACC TGTATATATG ATGTTACTGA TGAAAACTCT 
CATTTAAAAC TTATTTCTTC TCTTCAAGAC GAATGCTCTG ATGTTCTTTA TCTTTTAATG 
GAATTTCTTA ATATGGTTTG CTCTCCAAAG AGCTATTATA AATTGATTCC TTCATTTGAG 
CAATTAATTC AAGATTTTCA TATTCAACCT CAGGTTGCAT TTTATCTATC GCGTTACAAA 
AATTTAGATC ACAGTTTGAC TGGTTCAAAT ACTGAGGATG CAATGGATAT TGATTATGAA 
AATACTTCAT CTCCAAACAC TGCTAGCAAC CCCGTTTGGA GTATTGACAA TTCTGTCATA 
ACTGAGTTAT TACCTAAACA AATTTGGGAT TATTTTTCAC CTAATTTTTA TTTAACATTT 
TGGAAGCTAT CGCTGTATGA TGTTTTTGTT CCCTTGGAAC GTTATGAGTT TGAGCGTTCT 
CGGGCTTTTG ATCAAATTAG GCAAACTGAC GCCGCTAACA CATTTTATTC ACGACATCGA 
CACGACCGAC AAAAAATAAT GCAATTGTCT AACAGCTTAC AGAATGAACT TAAAGAACAC 
ATTAATTCAC TTGAGAGTGT AAGAAAAGTT TTGCAAGGTG ACTGCGTGAA ATGGTTTATT 
CCAAATGGTG TTTTTCCTAA TGGTACAAGA CTTGAGCATG CTAGATTCAA CTGTGCTCGG 
TATTTGTGGA CACTCTGCAT TGCTCCCCGT TTAAAAATGA GCCCACATGA TGCTTTGTAC 
TGCGCAAAGT TTGTAAAGCT TTTGCACAGT CTTGGAACAC CAAACTTTTC AACTATGTCA 
TTTTTAGAAA TTTTGTTTAA TTCACAATTG CCTTCATTTA TTTTTTCAAT GACACAACGA 
GAGGCTGATA ATTTTGGAAG ATTTTTATAC GAAGTACTCT ATGATATTAC TAGCTGGTAC 
AGGGACAAAA TTCTTTACGA ACGCGAATGT TTGGCAAATG GTGCTTTGCC TGGGTTCAGG 
TTATATTGGT CTGACGAACA AAATGATCCC GATCTTTCCG CTGTCCTTCC TTATAACAAA 
TTTGTTTTGC TATTTAGTAA ATGGCACAAA TATCTAACAT CTTATTTTGA GTCATGCTTA 
CTATCTACTG AATATATGCA TATCTACAAT AGTGTCATAA TCTTGGAAAA AATTTTGCCA 
TGTTTCCCAT TGATAATTGA AAGTGGCAGT GCTTTGAAAC GAGCCGCTGA ACGATTAAAG 
GACGAAGAAA AACGAGAAGA TTTAAAGGTT TTGGCTTTGG GGTATTTTGC AAAGCTCTCA 
AAAAAGCAAC CAGAATGGGT TTCTTTTAAT TCATTTTCTG GTACTGTAAG GCCGAGTAAT 
TCAGAAAAGC TTCAGAGACC GCAACAACTT TCAGTGGCAG CTACATCAGC AGTTGATTCC 
AAAACTGCTT CCATATCGGA AGAACAGGCA AAGATTGATA AACAGAAAGT AGCTTTGAAT 
CCTAGTGCAC CAGAGTTTGT ACCTGATAGT ACGCCTTCCG ACGCAGTTGC TTCTGAAACT 
GACAATAAAA ATCTTGTTGA GAACAAGGCA GTAGAGAAAA GGGTTGAAGC TAGAAGCTCA 
GCTAATGAAA GAAAACAAGA AGAAAGACGT AGAAAAACAA CTCCTGAAGG AAACAGACGT 
GCTTTACGAA CACGCACCCC TACGAATGAG GATATACAAA GATCAGACAG TAAATTGCGA 
GAGGATCAGT CTAGAGACCG AACACCTCAA TCAAGAAGTT TTACAAATGA AAACAACGAT 
AATTTAAGGA GCGTGTCTCG TCATACTCGA CGCGAGCCTC AGCAAGCACA AAACCTAAAT 
GCTAGGCGTG AACATGAATC ACAGAAATCT GACAGATGGC GCCAAAATGG AAACGTAAAT 
CGCAACCCTC GAGTGTCTAA TAACAATAGC ACTAATGTTT CAAGAGAGCG CTCGTCTGAA 
GCTAATCATA GGACAAGCAA CGATAATAAA CGTGATGAGG TTACAGAAGG GAAAGATAAA 
AACAAAAGGC AAGATATTTC TGGCGAGAGT AATTCTCGGC AGAATAATGC TATTAGTCGG 
GCTGGAAGAA GTAATGGATC TAATAGGGGA AATGATTCCA GAGATGCAGA TGGTCGTCGT 
AGCACTCATT ATGCTTCAAA TAAACGTCCC CGATCTTCGG ATAGTCAAAG CCCGAGCAAC 
CTACGAGAGG AAGATGAAAG AGAAAATTCT CGTCGTAGGG CCAGGCAAGA CGACCGAAGG 
GATCGGGATT CACGACAGCA AAGAGATCGT CCACGGGATA GAACATCTCG ATCTGCTCGA 
GAAGAGAAAA GAAGAAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THO2 ( XM_001712991.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> XP_001713043.1 Schizosaccharomyces pombe 972h- THO complex subunit Tho2 (predicted) (tho2), mRNA.

Price & Availability↑

CloneID	OSj04907
Clone ID Related Accession (Same CDS sequence)	XM_001712991.2
Accession Version	XM_001712991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	THO complex subunit Tho2 (predicted)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001712991.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGACTTCGT TGCCAGAAAA GGATCAGCAA GGCGAAGTTA GTGTTTCAGA AAATCAAAAG 
AAGCTCTCAG AGGAATGGGA GCCTTATATT GATAAACTTG TTGCATCTAA TAGAACGCGT 
GACGAACGTG TTGAATCAAT CCAAGAATTG TTTCAAAAAT GTGTCATTGA AGAGAAGTTA 
CCAGTAGACA TATTTTTCTC TATTTTTTCC TTAGCTTTTG AAAGATTGGA GGAAAAAAAT 
ACCTTAGCTT CTTATGTAAT TGATACGCTT TGGTTATTTG ACACAGAGTG GATCAAAAAC 
TTTCACGAAG GAAGTCATGA TAAAGCTGAA AAGAGGCTTG TTTCAATTGG AAAAGGACTA 
AAGGAATTCC TTCCAGAGGA ATGGTTGCTT TCTCGCTTGG ATTGCAAATT TTTGGAAAAT 
ATTAATGTCG TTCCTAATGG CGATTTTCTA AATCGGAAAA TTGTGCGGAC AAATACTTCG 
CTACTCTACC GCCAAAAAAA ATTTAATTTG CTTAGAGAAG AAAGTGAAGG ATTTTCACAT 
TTAATGATAA ACTTTTTTGA TGCTTTAACT TGTCTTCGCA ATAAATCCTT AAACGAAGAT 
TATCTGATCG TTCAAGTAAA TAAGTCAATT ATCAGTACTA TTGGTGCTTT TGATTTAGAT 
CCGAACAAAG TACTGGATTT GATTTTGTTG CTTTTTTCTG AAAATTTACT TGATTCGTGG 
CGCTTTTTCC TTTCTATCTT GAGAAACAGC CCATGGGGTC CGAATAAAGA GCGGAAATTT 
TGGAATCAGT TACCTGATCG CGAAAAGGAA ACATTTTTAA ATAATCTGTC TAATGCTAAT 
GGTATTTTTA ATTTTGACGA GCGATTCACC AATACAAAGA GCATAATGTC TCAAGTTTTG 
GGTCATAATT TGCAATATAT GTATAAAGAA GATGACGAGA ATTTGGAATC TTACTTTATG 
ATGGTTGCTT TGTTGATTAA ATATAATTTT ATTAGCATTG ATAACATATG GGCTCATCTT 
TCTCCATCAG ATGAAGAGCT TGGTAAAGAG TTGGGTAAAT ATAAGGATAA ATTAGATGAA 
CAGACTTTTA AGGCTAAAGG AAATGCTTTA ACAATGGCGG CCCCATTGCC CGACGATGAA 
ATTGAAGACG GAGAAACCAT GGATGGCCAG AAAGCTGAAG CTGTTCCTGA AATAAAAAAA 
GCCAAACCTT CACAGAAGCT GGGTCTACTA AAATCTTTAT TGTCCATCGG GGATTTATCT 
TCATCTCTAT TAATCTTGGG TCGTTATCCA TTTTTGTTGA GGGCGTACCC CGAATTATCT 
AACTTGTATC ACAAACTTTT GCATATTTCT ATTTCCTCAA TTTATGCAAA TTACAGTCCA 
TTGAAATTGT TACCAAATGA TGTAAGGGAA CGACTTAAAC AGCCAAAGTT CATTCCTGAA 
GACTCTAGGC TTCGAGAAAT TACCCTGAGG CCTCCAAAGG AAAAAAATTT AGTGTTTTCG 
TTGGATCCTT TTGCTGATCG ATTCAACAAA ACTGAAAGTG AGGTGTTTTA TTACTTTGAA 
AATTATGATG AAGATATACC AATTTTACGT AACTTGACAG AGTTCTACAA CATAGCTATC 
CCATGGTTAC GTTTGTCAGG GCTTGCTCTG TGTCATGATC CTGTTATAGT GACAAAGCTT 
TGCCGCATTG GTCAAAAATG TGTCGATAAT AGCTCTGAGA GTCGGACATT ATGGCTGGAT 
ATTATTAGAT CGCTTCTTCT TCCTCTCATC ACACTTATTG ATGTCAATAC TGGACTTTCC 
TATGAATTAT TTGAGTTGCT GTCAAAGTTT GATTCATCCA CTCGATATGC GTTGTATGGT 
GAATGGTCAA GTACCTCCAT GAAAAAGTTT CCGGAGCTAA AGCTACAAAA TTCTATCACT 
GAAAAGGAAA CAAAAGGCAT CTTAAGAAGG TTAACGAAAA CAAATGTGAA GCAATTTGGT 
AGATTATTGG CTAAAGTCTG TCATTCGAAT CCATGTACTG TGTTTTCGAT CGCTTTAAAT 
CAGATTGAAA CTTATGATAA TTTAGTTGAA GTTGTAGTTG ATAGTGCTCG TTTTATTACA 
GCATTAGACT TTGATGCATT GACATTTATT ATTTTATCTT CCTTTTCCAA TGAGTTCAAA 
AAGCGATTGA AATCTGATGG AACTAGTATA GCTCATTGGC TCCAAGGTTT AGCATCTTTT 
TGTGGCCGTG TATTCAGAAG ATACTCGAGC CTCGATTGCA CCTCTATCGT CGAATATGTG 
ATTAAACAGT TTAAAGTGAA CCAAATGTTT GACCTTGTTA TATTGAAAGA ATTGCTTTCG 
CAGATGACTG GGCTACAACC ATGGACAAAT CTCTCTGACA ATCAGATTCA AGGGGCTGCT 
GGTGGACCAG TTTTGCGACA ACTTAGTTTA TCTCTTATAT ATGAAAATCC GGACGTTGTT 
CGGAAAAGTT CCATGAGACT TTTTAATACG CTGCAAAAGA ACGGATTGGC AACCCAGCTA 
TTGGTACTAC TCTCTCAAAA ATATTCGACC TGTATATATG ATGTTACTGA TGAAAACTCT 
CATTTAAAAC TTATTTCTTC TCTTCAAGAC GAATGCTCTG ATGTTCTTTA TCTTTTAATG 
GAATTTCTTA ATATGGTTTG CTCTCCAAAG AGCTATTATA AATTGATTCC TTCATTTGAG 
CAATTAATTC AAGATTTTCA TATTCAACCT CAGGTTGCAT TTTATCTATC GCGTTACAAA 
AATTTAGATC ACAGTTTGAC TGGTTCAAAT ACTGAGGATG CAATGGATAT TGATTATGAA 
AATACTTCAT CTCCAAACAC TGCTAGCAAC CCCGTTTGGA GTATTGACAA TTCTGTCATA 
ACTGAGTTAT TACCTAAACA AATTTGGGAT TATTTTTCAC CTAATTTTTA TTTAACATTT 
TGGAAGCTAT CGCTGTATGA TGTTTTTGTT CCCTTGGAAC GTTATGAGTT TGAGCGTTCT 
CGGGCTTTTG ATCAAATTAG GCAAACTGAC GCCGCTAACA CATTTTATTC ACGACATCGA 
CACGACCGAC AAAAAATAAT GCAATTGTCT AACAGCTTAC AGAATGAACT TAAAGAACAC 
ATTAATTCAC TTGAGAGTGT AAGAAAAGTT TTGCAAGGTG ACTGCGTGAA ATGGTTTATT 
CCAAATGGTG TTTTTCCTAA TGGTACAAGA CTTGAGCATG CTAGATTCAA CTGTGCTCGG 
TATTTGTGGA CACTCTGCAT TGCTCCCCGT TTAAAAATGA GCCCACATGA TGCTTTGTAC 
TGCGCAAAGT TTGTAAAGCT TTTGCACAGT CTTGGAACAC CAAACTTTTC AACTATGTCA 
TTTTTAGAAA TTTTGTTTAA TTCACAATTG CCTTCATTTA TTTTTTCAAT GACACAACGA 
GAGGCTGATA ATTTTGGAAG ATTTTTATAC GAAGTACTCT ATGATATTAC TAGCTGGTAC 
AGGGACAAAA TTCTTTACGA ACGCGAATGT TTGGCAAATG GTGCTTTGCC TGGGTTCAGG 
TTATATTGGT CTGACGAACA AAATGATCCC GATCTTTCCG CTGTCCTTCC TTATAACAAA 
TTTGTTTTGC TATTTAGTAA ATGGCACAAA TATCTAACAT CTTATTTTGA GTCATGCTTA 
CTATCTACTG AATATATGCA TATCTACAAT AGTGTCATAA TCTTGGAAAA AATTTTGCCA 
TGTTTCCCAT TGATAATTGA AAGTGGCAGT GCTTTGAAAC GAGCCGCTGA ACGATTAAAG 
GACGAAGAAA AACGAGAAGA TTTAAAGGTT TTGGCTTTGG GGTATTTTGC AAAGCTCTCA 
AAAAAGCAAC CAGAATGGGT TTCTTTTAAT TCATTTTCTG GTACTGTAAG GCCGAGTAAT 
TCAGAAAAGC TTCAGAGACC GCAACAACTT TCAGTGGCAG CTACATCAGC AGTTGATTCC 
AAAACTGCTT CCATATCGGA AGAACAGGCA AAGATTGATA AACAGAAAGT AGCTTTGAAT 
CCTAGTGCAC CAGAGTTTGT ACCTGATAGT ACGCCTTCCG ACGCAGTTGC TTCTGAAACT 
GACAATAAAA ATCTTGTTGA GAACAAGGCA GTAGAGAAAA GGGTTGAAGC TAGAAGCTCA 
GCTAATGAAA GAAAACAAGA AGAAAGACGT AGAAAAACAA CTCCTGAAGG AAACAGACGT 
GCTTTACGAA CACGCACCCC TACGAATGAG GATATACAAA GATCAGACAG TAAATTGCGA 
GAGGATCAGT CTAGAGACCG AACACCTCAA TCAAGAAGTT TTACAAATGA AAACAACGAT 
AATTTAAGGA GCGTGTCTCG TCATACTCGA CGCGAGCCTC AGCAAGCACA AAACCTAAAT 
GCTAGGCGTG AACATGAATC ACAGAAATCT GACAGATGGC GCCAAAATGG AAACGTAAAT 
CGCAACCCTC GAGTGTCTAA TAACAATAGC ACTAATGTTT CAAGAGAGCG CTCGTCTGAA 
GCTAATCATA GGACAAGCAA CGATAATAAA CGTGATGAGG TTACAGAAGG GAAAGATAAA 
AACAAAAGGC AAGATATTTC TGGCGAGAGT AATTCTCGGC AGAATAATGC TATTAGTCGG 
GCTGGAAGAA GTAATGGATC TAATAGGGGA AATGATTCCA GAGATGCAGA TGGTCGTCGT 
AGCACTCATT ATGCTTCAAA TAAACGTCCC CGATCTTCGG ATAGTCAAAG CCCGAGCAAC 
CTACGAGAGG AAGATGAAAG AGAAAATTCT CGTCGTAGGG CCAGGCAAGA CGACCGAAGG 
GATCGGGATT CACGACAGCA AAGAGATCGT CCACGGGATA GAACATCTCG ATCTGCTCGA 
GAAGAGAAAA GAAGAAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713043.1
CDS	177..5063
Translation	MTSLPEKDQQGEVSVSENQKKLSEEWEPYIDKLVASNRTRDERVESIQELFQKCVIEEKLPVDIFFSIFSLAFERLEEKNTLASYVIDTLWLFDTEWIKNFHEGSHDKAEKRLVSIGKGLKEFLPEEWLLSRLDCKFLENINVVPNGDFLNRKIVRTNTSLLYRQKKFNLLREESEGFSHLMINFFDALTCLRNKSLNEDYLIVQVNKSIISTIGAFDLDPNKVLDLILLLFSENLLDSWRFFLSILRNSPWGPNKERKFWNQLPDREKETFLNNLSNANGIFNFDERFTNTKSIMSQVLGHNLQYMYKEDDENLESYFMMVALLIKYNFISIDNIWAHLSPSDEELGKELGKYKDKLDEQTFKAKGNALTMAAPLPDDEIEDGETMDGQKAEAVPEIKKAKPSQKLGLLKSLLSIGDLSSSLLILGRYPFLLRAYPELSNLYHKLLHISISSIYANYSPLKLLPNDVRERLKQPKFIPEDSRLREITLRPPKEKNLVFSLDPFADRFNKTESEVFYYFENYDEDIPILRNLTEFYNIAIPWLRLSGLALCHDPVIVTKLCRIGQKCVDNSSESRTLWLDIIRSLLLPLITLIDVNTGLSYELFELLSKFDSSTRYALYGEWSSTSMKKFPELKLQNSITEKETKGILRRLTKTNVKQFGRLLAKVCHSNPCTVFSIALNQIETYDNLVEVVVDSARFITALDFDALTFIILSSFSNEFKKRLKSDGTSIAHWLQGLASFCGRVFRRYSSLDCTSIVEYVIKQFKVNQMFDLVILKELLSQMTGLQPWTNLSDNQIQGAAGGPVLRQLSLSLIYENPDVVRKSSMRLFNTLQKNGLATQLLVLLSQKYSTCIYDVTDENSHLKLISSLQDECSDVLYLLMEFLNMVCSPKSYYKLIPSFEQLIQDFHIQPQVAFYLSRYKNLDHSLTGSNTEDAMDIDYENTSSPNTASNPVWSIDNSVITELLPKQIWDYFSPNFYLTFWKLSLYDVFVPLERYEFERSRAFDQIRQTDAANTFYSRHRHDRQKIMQLSNSLQNELKEHINSLESVRKVLQGDCVKWFIPNGVFPNGTRLEHARFNCARYLWTLCIAPRLKMSPHDALYCAKFVKLLHSLGTPNFSTMSFLEILFNSQLPSFIFSMTQREADNFGRFLYEVLYDITSWYRDKILYERECLANGALPGFRLYWSDEQNDPDLSAVLPYNKFVLLFSKWHKYLTSYFESCLLSTEYMHIYNSVIILEKILPCFPLIIESGSALKRAAERLKDEEKREDLKVLALGYFAKLSKKQPEWVSFNSFSGTVRPSNSEKLQRPQQLSVAATSAVDSKTASISEEQAKIDKQKVALNPSAPEFVPDSTPSDAVASETDNKNLVENKAVEKRVEARSSANERKQEERRRKTTPEGNRRALRTRTPTNEDIQRSDSKLREDQSRDRTPQSRSFTNENNDNLRSVSRHTRREPQQAQNLNARREHESQKSDRWRQNGNVNRNPRVSNNNSTNVSRERSSEANHRTSNDNKRDEVTEGKDKNKRQDISGESNSRQNNAISRAGRSNGSNRGNDSRDADGRRSTHYASNKRPRSSDSQSPSNLREEDERENSRRRARQDDRRDRDSRQQRDRPRDRTSRSAREEKRRKIQ

Target ORF information:

RefSeq Version	XM_001712991.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- THO complex subunit Tho2 (predicted) (tho2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001712991.2

ORF Insert Sequence:

ATGACTTCGT TGCCAGAAAA GGATCAGCAA GGCGAAGTTA GTGTTTCAGA AAATCAAAAG 
AAGCTCTCAG AGGAATGGGA GCCTTATATT GATAAACTTG TTGCATCTAA TAGAACGCGT 
GACGAACGTG TTGAATCAAT CCAAGAATTG TTTCAAAAAT GTGTCATTGA AGAGAAGTTA 
CCAGTAGACA TATTTTTCTC TATTTTTTCC TTAGCTTTTG AAAGATTGGA GGAAAAAAAT 
ACCTTAGCTT CTTATGTAAT TGATACGCTT TGGTTATTTG ACACAGAGTG GATCAAAAAC 
TTTCACGAAG GAAGTCATGA TAAAGCTGAA AAGAGGCTTG TTTCAATTGG AAAAGGACTA 
AAGGAATTCC TTCCAGAGGA ATGGTTGCTT TCTCGCTTGG ATTGCAAATT TTTGGAAAAT 
ATTAATGTCG TTCCTAATGG CGATTTTCTA AATCGGAAAA TTGTGCGGAC AAATACTTCG 
CTACTCTACC GCCAAAAAAA ATTTAATTTG CTTAGAGAAG AAAGTGAAGG ATTTTCACAT 
TTAATGATAA ACTTTTTTGA TGCTTTAACT TGTCTTCGCA ATAAATCCTT AAACGAAGAT 
TATCTGATCG TTCAAGTAAA TAAGTCAATT ATCAGTACTA TTGGTGCTTT TGATTTAGAT 
CCGAACAAAG TACTGGATTT GATTTTGTTG CTTTTTTCTG AAAATTTACT TGATTCGTGG 
CGCTTTTTCC TTTCTATCTT GAGAAACAGC CCATGGGGTC CGAATAAAGA GCGGAAATTT 
TGGAATCAGT TACCTGATCG CGAAAAGGAA ACATTTTTAA ATAATCTGTC TAATGCTAAT 
GGTATTTTTA ATTTTGACGA GCGATTCACC AATACAAAGA GCATAATGTC TCAAGTTTTG 
GGTCATAATT TGCAATATAT GTATAAAGAA GATGACGAGA ATTTGGAATC TTACTTTATG 
ATGGTTGCTT TGTTGATTAA ATATAATTTT ATTAGCATTG ATAACATATG GGCTCATCTT 
TCTCCATCAG ATGAAGAGCT TGGTAAAGAG TTGGGTAAAT ATAAGGATAA ATTAGATGAA 
CAGACTTTTA AGGCTAAAGG AAATGCTTTA ACAATGGCGG CCCCATTGCC CGACGATGAA 
ATTGAAGACG GAGAAACCAT GGATGGCCAG AAAGCTGAAG CTGTTCCTGA AATAAAAAAA 
GCCAAACCTT CACAGAAGCT GGGTCTACTA AAATCTTTAT TGTCCATCGG GGATTTATCT 
TCATCTCTAT TAATCTTGGG TCGTTATCCA TTTTTGTTGA GGGCGTACCC CGAATTATCT 
AACTTGTATC ACAAACTTTT GCATATTTCT ATTTCCTCAA TTTATGCAAA TTACAGTCCA 
TTGAAATTGT TACCAAATGA TGTAAGGGAA CGACTTAAAC AGCCAAAGTT CATTCCTGAA 
GACTCTAGGC TTCGAGAAAT TACCCTGAGG CCTCCAAAGG AAAAAAATTT AGTGTTTTCG 
TTGGATCCTT TTGCTGATCG ATTCAACAAA ACTGAAAGTG AGGTGTTTTA TTACTTTGAA 
AATTATGATG AAGATATACC AATTTTACGT AACTTGACAG AGTTCTACAA CATAGCTATC 
CCATGGTTAC GTTTGTCAGG GCTTGCTCTG TGTCATGATC CTGTTATAGT GACAAAGCTT 
TGCCGCATTG GTCAAAAATG TGTCGATAAT AGCTCTGAGA GTCGGACATT ATGGCTGGAT 
ATTATTAGAT CGCTTCTTCT TCCTCTCATC ACACTTATTG ATGTCAATAC TGGACTTTCC 
TATGAATTAT TTGAGTTGCT GTCAAAGTTT GATTCATCCA CTCGATATGC GTTGTATGGT 
GAATGGTCAA GTACCTCCAT GAAAAAGTTT CCGGAGCTAA AGCTACAAAA TTCTATCACT 
GAAAAGGAAA CAAAAGGCAT CTTAAGAAGG TTAACGAAAA CAAATGTGAA GCAATTTGGT 
AGATTATTGG CTAAAGTCTG TCATTCGAAT CCATGTACTG TGTTTTCGAT CGCTTTAAAT 
CAGATTGAAA CTTATGATAA TTTAGTTGAA GTTGTAGTTG ATAGTGCTCG TTTTATTACA 
GCATTAGACT TTGATGCATT GACATTTATT ATTTTATCTT CCTTTTCCAA TGAGTTCAAA 
AAGCGATTGA AATCTGATGG AACTAGTATA GCTCATTGGC TCCAAGGTTT AGCATCTTTT 
TGTGGCCGTG TATTCAGAAG ATACTCGAGC CTCGATTGCA CCTCTATCGT CGAATATGTG 
ATTAAACAGT TTAAAGTGAA CCAAATGTTT GACCTTGTTA TATTGAAAGA ATTGCTTTCG 
CAGATGACTG GGCTACAACC ATGGACAAAT CTCTCTGACA ATCAGATTCA AGGGGCTGCT 
GGTGGACCAG TTTTGCGACA ACTTAGTTTA TCTCTTATAT ATGAAAATCC GGACGTTGTT 
CGGAAAAGTT CCATGAGACT TTTTAATACG CTGCAAAAGA ACGGATTGGC AACCCAGCTA 
TTGGTACTAC TCTCTCAAAA ATATTCGACC TGTATATATG ATGTTACTGA TGAAAACTCT 
CATTTAAAAC TTATTTCTTC TCTTCAAGAC GAATGCTCTG ATGTTCTTTA TCTTTTAATG 
GAATTTCTTA ATATGGTTTG CTCTCCAAAG AGCTATTATA AATTGATTCC TTCATTTGAG 
CAATTAATTC AAGATTTTCA TATTCAACCT CAGGTTGCAT TTTATCTATC GCGTTACAAA 
AATTTAGATC ACAGTTTGAC TGGTTCAAAT ACTGAGGATG CAATGGATAT TGATTATGAA 
AATACTTCAT CTCCAAACAC TGCTAGCAAC CCCGTTTGGA GTATTGACAA TTCTGTCATA 
ACTGAGTTAT TACCTAAACA AATTTGGGAT TATTTTTCAC CTAATTTTTA TTTAACATTT 
TGGAAGCTAT CGCTGTATGA TGTTTTTGTT CCCTTGGAAC GTTATGAGTT TGAGCGTTCT 
CGGGCTTTTG ATCAAATTAG GCAAACTGAC GCCGCTAACA CATTTTATTC ACGACATCGA 
CACGACCGAC AAAAAATAAT GCAATTGTCT AACAGCTTAC AGAATGAACT TAAAGAACAC 
ATTAATTCAC TTGAGAGTGT AAGAAAAGTT TTGCAAGGTG ACTGCGTGAA ATGGTTTATT 
CCAAATGGTG TTTTTCCTAA TGGTACAAGA CTTGAGCATG CTAGATTCAA CTGTGCTCGG 
TATTTGTGGA CACTCTGCAT TGCTCCCCGT TTAAAAATGA GCCCACATGA TGCTTTGTAC 
TGCGCAAAGT TTGTAAAGCT TTTGCACAGT CTTGGAACAC CAAACTTTTC AACTATGTCA 
TTTTTAGAAA TTTTGTTTAA TTCACAATTG CCTTCATTTA TTTTTTCAAT GACACAACGA 
GAGGCTGATA ATTTTGGAAG ATTTTTATAC GAAGTACTCT ATGATATTAC TAGCTGGTAC 
AGGGACAAAA TTCTTTACGA ACGCGAATGT TTGGCAAATG GTGCTTTGCC TGGGTTCAGG 
TTATATTGGT CTGACGAACA AAATGATCCC GATCTTTCCG CTGTCCTTCC TTATAACAAA 
TTTGTTTTGC TATTTAGTAA ATGGCACAAA TATCTAACAT CTTATTTTGA GTCATGCTTA 
CTATCTACTG AATATATGCA TATCTACAAT AGTGTCATAA TCTTGGAAAA AATTTTGCCA 
TGTTTCCCAT TGATAATTGA AAGTGGCAGT GCTTTGAAAC GAGCCGCTGA ACGATTAAAG 
GACGAAGAAA AACGAGAAGA TTTAAAGGTT TTGGCTTTGG GGTATTTTGC AAAGCTCTCA 
AAAAAGCAAC CAGAATGGGT TTCTTTTAAT TCATTTTCTG GTACTGTAAG GCCGAGTAAT 
TCAGAAAAGC TTCAGAGACC GCAACAACTT TCAGTGGCAG CTACATCAGC AGTTGATTCC 
AAAACTGCTT CCATATCGGA AGAACAGGCA AAGATTGATA AACAGAAAGT AGCTTTGAAT 
CCTAGTGCAC CAGAGTTTGT ACCTGATAGT ACGCCTTCCG ACGCAGTTGC TTCTGAAACT 
GACAATAAAA ATCTTGTTGA GAACAAGGCA GTAGAGAAAA GGGTTGAAGC TAGAAGCTCA 
GCTAATGAAA GAAAACAAGA AGAAAGACGT AGAAAAACAA CTCCTGAAGG AAACAGACGT 
GCTTTACGAA CACGCACCCC TACGAATGAG GATATACAAA GATCAGACAG TAAATTGCGA 
GAGGATCAGT CTAGAGACCG AACACCTCAA TCAAGAAGTT TTACAAATGA AAACAACGAT 
AATTTAAGGA GCGTGTCTCG TCATACTCGA CGCGAGCCTC AGCAAGCACA AAACCTAAAT 
GCTAGGCGTG AACATGAATC ACAGAAATCT GACAGATGGC GCCAAAATGG AAACGTAAAT 
CGCAACCCTC GAGTGTCTAA TAACAATAGC ACTAATGTTT CAAGAGAGCG CTCGTCTGAA 
GCTAATCATA GGACAAGCAA CGATAATAAA CGTGATGAGG TTACAGAAGG GAAAGATAAA 
AACAAAAGGC AAGATATTTC TGGCGAGAGT AATTCTCGGC AGAATAATGC TATTAGTCGG 
GCTGGAAGAA GTAATGGATC TAATAGGGGA AATGATTCCA GAGATGCAGA TGGTCGTCGT 
AGCACTCATT ATGCTTCAAA TAAACGTCCC CGATCTTCGG ATAGTCAAAG CCCGAGCAAC 
CTACGAGAGG AAGATGAAAG AGAAAATTCT CGTCGTAGGG CCAGGCAAGA CGACCGAAGG 
GATCGGGATT CACGACAGCA AAGAGATCGT CCACGGGATA GAACATCTCG ATCTGCTCGA 
GAAGAGAAAA GAAGAAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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THO2 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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