SPBC25H2.10c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC25H2.10c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC25H2.10c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100112 NM_001022277.1
Latest version!
Schizosaccharomyces pombe putative tRNA acetyltransferase (SPBC25H2.10c), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100112
    Clone ID Related Accession (Same CDS sequence) NM_001022277.1
    Accession Version NM_001022277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative tRNA acetyltransferase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGTGCAT TGTCCGAAAT ATTAAGTTAC CTAATTATTT CATTTGAGAC GAGTTTTACC 
    TTTCACCTTG AATTTAAGTA CAAAAGAACA GGGATTGAAA AAAATGAAAG TGGTGTTTTA
    GTTACTGTTC CTCGCGGTAA AGAAAGACAA TCATCGTCTG AGATTCTTGA TGTTTTTACT
    CAGCATATAG CTACTCTTTG GCCAGAAACT CAATCTAGTG ATCAAGAAAA CGAAGAAGAA
    GGCGAGGAAT TAGAAGATGC AGTTGCTAAG GAGATTAATA GTCTCCAAAA AAAGAACAAA
    AAAGAACTGT TAACCCCAAT TATGCTAGAT ATGCCTTGTG TTTACTTTGT AAAAACAAGG
    CCACCAATTG ATCCAGTGCG TCTTGTAGAG TTTACTTGTG AAGTTGGGAA AACCAAAAAA
    ATGACTCGAT ATACACAACG ATTGATACCG ATTGTAAGAA CAACTGGCGT TTCCCTGGAT
    GATCTCGAGG AGCTTGCTAA AAGTCTGATT GACCCGTTAT TTCATGAAGG CCAGGAAGGG
    ATTAAGGAGT TCGCTGTTCA AGCCAATATC CGAAATCATA CAGTCCTAAA AAAAGATGAT
    ATATATCGCA CTGTTGCTAG AATCGTTGGA AAGCAACATA TGGTAGATTT GAAGAACTTT
    AAATTGCTTA TTTTAGTCCA AGTGATTAAG AATATAATTG GCATAAGTAT TGTACAAAAT
    TTCGAAGAGT TGAGACGCTT CAATTTGAAT GAAGTATATA AACAACCGGA GAATACCAAG
    TCGATCCCTA ATGATTCTAA GTTAGACAAC TTCGATCGCG ATAAGAATCA AATAATAAAT
    GATAAGGCCG AACATGCTGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001018836.1
    CDS1..864
    Translation

    Target ORF information:

    RefSeq Version NM_001022277.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative tRNA acetyltransferase (SPBC25H2.10c), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGTGCAT TGTCCGAAAT ATTAAGTTAC CTAATTATTT CATTTGAGAC GAGTTTTACC 
    TTTCACCTTG AATTTAAGTA CAAAAGAACA GGGATTGAAA AAAATGAAAG TGGTGTTTTA
    GTTACTGTTC CTCGCGGTAA AGAAAGACAA TCATCGTCTG AGATTCTTGA TGTTTTTACT
    CAGCATATAG CTACTCTTTG GCCAGAAACT CAATCTAGTG ATCAAGAAAA CGAAGAAGAA
    GGCGAGGAAT TAGAAGATGC AGTTGCTAAG GAGATTAATA GTCTCCAAAA AAAGAACAAA
    AAAGAACTGT TAACCCCAAT TATGCTAGAT ATGCCTTGTG TTTACTTTGT AAAAACAAGG
    CCACCAATTG ATCCAGTGCG TCTTGTAGAG TTTACTTGTG AAGTTGGGAA AACCAAAAAA
    ATGACTCGAT ATACACAACG ATTGATACCG ATTGTAAGAA CAACTGGCGT TTCCCTGGAT
    GATCTCGAGG AGCTTGCTAA AAGTCTGATT GACCCGTTAT TTCATGAAGG CCAGGAAGGG
    ATTAAGGAGT TCGCTGTTCA AGCCAATATC CGAAATCATA CAGTCCTAAA AAAAGATGAT
    ATATATCGCA CTGTTGCTAG AATCGTTGGA AAGCAACATA TGGTAGATTT GAAGAACTTT
    AAATTGCTTA TTTTAGTCCA AGTGATTAAG AATATAATTG GCATAAGTAT TGTACAAAAT
    TTCGAAGAGT TGAGACGCTT CAATTTGAAT GAAGTATATA AACAACCGGA GAATACCAAG
    TCGATCCCTA ATGATTCTAA GTTAGACAAC TTCGATCGCG ATAAGAATCA AATAATAAAT
    GATAAGGCCG AACATGCTGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L