ORF » Species Summary » Schizosaccharomyces pombe » spt20 cDNA ORF clone

spt20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following spt20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spt20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj04817 NM_001020394.2
Latest version!		 Schizosaccharomyces pombe 972h- SAGA complex subunit Spt20 (spt20), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OSc102400 NM_001356204.1
Latest version!		 Schizosaccharomyces pombe SAGA complex subunit Spt20 (spt20), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    spt20 ( NM_001020394.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594963.1 Schizosaccharomyces pombe 972h- SAGA complex subunit Spt20 (spt20), mRNA.
    Price & Availability↑
    CloneID OSj04817
    Clone ID Related Accession (Same CDS sequence) NM_001020394.2
    Accession Version NM_001020394.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product SAGA complex subunit Spt20
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020394.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAGAA ATAATGGTTT GGGCGACTAT ACGTATCGCT ATCATGGGAA GGAATTGAGT 
    TTGGACGATT TTCAGAATAA ACAAGGAATT GAATCCTCAG ATGATGCATT TTTATCAAAA 
    ATTTATGAAA ACGTTTCGAA TTTTAATGTT CCCAGAAAAT TGCATGATAT TGAACATAAA 
    TTTTCGAAAG AAGAGCCGAG TTTAATTCTT CATATTCATA AATTTCATTT TCGGTTTGAG 
    CAACAAGATG GCGCCTTCAC CTATAATGGG CCGGTAAAAT CAATTTTGCA ATATATAAGG 
    ATGGAGCTTA TACCACCTGA TTGTCTTGAA GTATTTCGGA ATTCCGATGT AAAATTCTAT 
    GATGGTTGCT TAACGGTTCG TATAATAGAT CATCGGCAAA GTCCTTCTGC TGATCAGACT 
    GTGCAACCTC AGCCTGGCTC TACTAATCAA CAGCAGCAAA ATAACACCAA CCCAATTAAT 
    AATCAACCTG AAGATACCAA ACCCAATACT AATTCACCTC CAGTGTATCA TACAGTTCTT 
    CGTCCTACTC CCGAAACACT TTGGCAAGAT TTATGTTTAC TTTCAGAAAG CTTTGCTAAC 
    TCACTTTCGG ATGAAGCTGT TTTGACTTTG GAATCCAACA TTCTACTAGC GTCAGAAGCT 
    CCACTTTTCT TAACTCCTGC GAAGTCTAAA GCGGAAATGA TTCAATTTAT GAACCAGTTG 
    GCCGACTCTG CACCACCATG CACCAGAAAG AAACCTCAAG GATCAGCCCA ACTTGCTGAT 
    GAAGAAGCAG AAAGACTTGA AAAAGAGAAC CTTTTGTTAC TAATGGATGA CCAAAGAAAA 
    CGCGATTTTC AACCTACCTT TCAAAGACTA CAATTTATTG AGAATGTGCG GCGTAAACGA 
    GCTATTCTAC AACAAAGGCA AATGCAAATG CAACAGCAAC AAAAAGCGCA GCAGCAGCAA 
    TCACCGAAAG CTCAGCAACC ACCCGCACAT TTGGTTCAGT CTGCTCCAGT TCAAAGAAAA 
    ACTACACCCA AAATCCAACG TCTACCACCT AGTAGTATTC AAATTCCCCC TCCGAAACCT 
    ATGCAGAAAT TCCCAGCTAA TGCGGCATCT TCTGAATCGC CCCCCAATGC GACTGGAAAC 
    TTTTTACCTT CGGGTCCTGT ACCTGCAAAT GAACCTATGT TAAAACGAGA ATCCGTGGAT 
    TTGATAAAAA TACGACAACT TGCAATTTTG TTTCAACAGC GTGCCTCTCA ATTAAAAGCT 
    AGAGGTGCTA CACGGGAACA AATTACTGAA ATTCTGAATC GTCAAGCAAT AGCCGCTGGT 
    ACTGATTTGG CAACAGTAAT GACGGTTGCA CGAAATCTTC ATTTTCAACA ACTCCAAATG 
    AGACAGCAGC AACAACAGCA GCAAATGAAG GCTGAAAGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594963.1
    CDS105..1526
    Translation

    Target ORF information:

    RefSeq Version NM_001020394.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- SAGA complex subunit Spt20 (spt20), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001020394.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAGAA ATAATGGTTT GGGCGACTAT ACGTATCGCT ATCATGGGAA GGAATTGAGT 
    TTGGACGATT TTCAGAATAA ACAAGGAATT GAATCCTCAG ATGATGCATT TTTATCAAAA 
    ATTTATGAAA ACGTTTCGAA TTTTAATGTT CCCAGAAAAT TGCATGATAT TGAACATAAA 
    TTTTCGAAAG AAGAGCCGAG TTTAATTCTT CATATTCATA AATTTCATTT TCGGTTTGAG 
    CAACAAGATG GCGCCTTCAC CTATAATGGG CCGGTAAAAT CAATTTTGCA ATATATAAGG 
    ATGGAGCTTA TACCACCTGA TTGTCTTGAA GTATTTCGGA ATTCCGATGT AAAATTCTAT 
    GATGGTTGCT TAACGGTTCG TATAATAGAT CATCGGCAAA GTCCTTCTGC TGATCAGACT 
    GTGCAACCTC AGCCTGGCTC TACTAATCAA CAGCAGCAAA ATAACACCAA CCCAATTAAT 
    AATCAACCTG AAGATACCAA ACCCAATACT AATTCACCTC CAGTGTATCA TACAGTTCTT 
    CGTCCTACTC CCGAAACACT TTGGCAAGAT TTATGTTTAC TTTCAGAAAG CTTTGCTAAC 
    TCACTTTCGG ATGAAGCTGT TTTGACTTTG GAATCCAACA TTCTACTAGC GTCAGAAGCT 
    CCACTTTTCT TAACTCCTGC GAAGTCTAAA GCGGAAATGA TTCAATTTAT GAACCAGTTG 
    GCCGACTCTG CACCACCATG CACCAGAAAG AAACCTCAAG GATCAGCCCA ACTTGCTGAT 
    GAAGAAGCAG AAAGACTTGA AAAAGAGAAC CTTTTGTTAC TAATGGATGA CCAAAGAAAA 
    CGCGATTTTC AACCTACCTT TCAAAGACTA CAATTTATTG AGAATGTGCG GCGTAAACGA 
    GCTATTCTAC AACAAAGGCA AATGCAAATG CAACAGCAAC AAAAAGCGCA GCAGCAGCAA 
    TCACCGAAAG CTCAGCAACC ACCCGCACAT TTGGTTCAGT CTGCTCCAGT TCAAAGAAAA 
    ACTACACCCA AAATCCAACG TCTACCACCT AGTAGTATTC AAATTCCCCC TCCGAAACCT 
    ATGCAGAAAT TCCCAGCTAA TGCGGCATCT TCTGAATCGC CCCCCAATGC GACTGGAAAC 
    TTTTTACCTT CGGGTCCTGT ACCTGCAAAT GAACCTATGT TAAAACGAGA ATCCGTGGAT 
    TTGATAAAAA TACGACAACT TGCAATTTTG TTTCAACAGC GTGCCTCTCA ATTAAAAGCT 
    AGAGGTGCTA CACGGGAACA AATTACTGAA ATTCTGAATC GTCAAGCAAT AGCCGCTGGT 
    ACTGATTTGG CAACAGTAAT GACGGTTGCA CGAAATCTTC ATTTTCAACA ACTCCAAATG 
    AGACAGCAGC AACAACAGCA GCAAATGAAG GCTGAAAGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spt20 ( NM_001356204.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342846.1 Schizosaccharomyces pombe SAGA complex subunit Spt20 (spt20), mRNA.
    Price & Availability↑
    CloneID OSc102400
    Clone ID Related Accession (Same CDS sequence) NM_001356204.1
    Accession Version NM_001356204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product SAGA complex subunit Spt20
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAGAA ATAATGGTTT GGGCGACTAT ACGTATCGCT ATCATGGGAA GGAATTGAGT 
    TTGGACGATT TTCAGAATAA ACAAGGAATT GAATCCTCAG ATGATGCATT TTTATCAAAA 
    ATTTATGAAA ACGTTTCGAA TTTTAATGTT CCCAGAAAAT TGCATGATAT TGAACATAAA 
    TTTTCGAAAG AAGAGCCGAG TTTAATTCTT CATATTCATA AATTTCATTT TCGGTTTGAG 
    CAACAAGATG GCGCCTTCAC CTATAATGGG CCGGTAAAAT CAATTTTGCA ATATATAAGG 
    ATGGAGCTTA TACCACCTGA TTGTCTTGAA GTATTTCGGA ATTCCGATGT AAAATTCTAT 
    GATGGTTGCT TAACGGTTCG TATAATAGAT CATCGGCAAA GTCCTTCTGC TGATCAGACT 
    GTGCAACCTC AGCCTGGCTC TACTAATCAA CAGCAGCAAA ATAACACCAA CCCAATTAAT 
    AATCAACCTG AAGATACCAA ACCCAATACT AATTCACCTC CAGTGTATCA TACAGTTCTT 
    CGTCCTACTC CCGAAACACT TTGGCAAGAT TTATGTTTAC TTTCAGAAAG CTTTGCTAAC 
    TCACTTTCGG ATGAAGCTGT TTTGACTTTG GAATCCAACA TTCTACTAGC GTCAGAAGCT 
    CCACTTTTCT TAACTCCTGC GAAGTCTAAA GCGGAAATGA TTCAATTTAT GAACCAGTTG 
    GCCGACTCTG CACCACCATG CACCAGAAAG AAACCTCAAG GATCAGCCCA ACTTGCTGAT 
    GAAGAAGCAG AAAGACTTGA AAAAGAGAAC CTTTTGTTAC TAATGGATGA CCAAAGAAAA 
    CGCGATTTTC AACCTACCTT TCAAAGACTA CAATTTATTG AGAATGTGCG GCGTAAACGA 
    GCTATTCTAC AACAAAGGCA AATGCAAATG CAACAGCAAC AAAAAGCGCA GCAGCAGCAA 
    TCACCGAAAG CTCAGCAACC ACCCGCACAT TTGGTTCAGT CTGCTCCAGT TCAAAGAAAA 
    ACTACACCCA AAATCCAACG TCTACCACCT AGTAGTATTC AAATTCCCCC TCCGAAACCT 
    ATGCAGAAAT TCCCAGCTAA TGCGGCATCT TCTGAATCGC CCCCCAATGC GACTGGAAAC 
    TTTTTACCTT CGGGTCCTGT ACCTGCAAAT GAACCTATGT TAAAACGAGA ATCCGTGGAT 
    TTGATAAAAA TACGACAACT TGCAATTTTG TTTCAACAGC GTGCCTCTCA ATTAAAAGCT 
    AGAGGTGCTA CACGGGAACA AATTACTGAA ATTCTGAATC GTCAAGCAAT AGCCGCTGGT 
    ACTGATTTGG CAACAGTAAT GACGGTTGCA CGAAATCTTC ATTTTCAACA ACTCCAAATG 
    AGACAGCAGC AACAACAGCA GCAAATGAAG GCTGAAAGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342846.1
    CDS105..1526
    Translation

    Target ORF information:

    RefSeq Version NM_001356204.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe SAGA complex subunit Spt20 (spt20), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001356204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAGAA ATAATGGTTT GGGCGACTAT ACGTATCGCT ATCATGGGAA GGAATTGAGT 
    TTGGACGATT TTCAGAATAA ACAAGGAATT GAATCCTCAG ATGATGCATT TTTATCAAAA 
    ATTTATGAAA ACGTTTCGAA TTTTAATGTT CCCAGAAAAT TGCATGATAT TGAACATAAA 
    TTTTCGAAAG AAGAGCCGAG TTTAATTCTT CATATTCATA AATTTCATTT TCGGTTTGAG 
    CAACAAGATG GCGCCTTCAC CTATAATGGG CCGGTAAAAT CAATTTTGCA ATATATAAGG 
    ATGGAGCTTA TACCACCTGA TTGTCTTGAA GTATTTCGGA ATTCCGATGT AAAATTCTAT 
    GATGGTTGCT TAACGGTTCG TATAATAGAT CATCGGCAAA GTCCTTCTGC TGATCAGACT 
    GTGCAACCTC AGCCTGGCTC TACTAATCAA CAGCAGCAAA ATAACACCAA CCCAATTAAT 
    AATCAACCTG AAGATACCAA ACCCAATACT AATTCACCTC CAGTGTATCA TACAGTTCTT 
    CGTCCTACTC CCGAAACACT TTGGCAAGAT TTATGTTTAC TTTCAGAAAG CTTTGCTAAC 
    TCACTTTCGG ATGAAGCTGT TTTGACTTTG GAATCCAACA TTCTACTAGC GTCAGAAGCT 
    CCACTTTTCT TAACTCCTGC GAAGTCTAAA GCGGAAATGA TTCAATTTAT GAACCAGTTG 
    GCCGACTCTG CACCACCATG CACCAGAAAG AAACCTCAAG GATCAGCCCA ACTTGCTGAT 
    GAAGAAGCAG AAAGACTTGA AAAAGAGAAC CTTTTGTTAC TAATGGATGA CCAAAGAAAA 
    CGCGATTTTC AACCTACCTT TCAAAGACTA CAATTTATTG AGAATGTGCG GCGTAAACGA 
    GCTATTCTAC AACAAAGGCA AATGCAAATG CAACAGCAAC AAAAAGCGCA GCAGCAGCAA 
    TCACCGAAAG CTCAGCAACC ACCCGCACAT TTGGTTCAGT CTGCTCCAGT TCAAAGAAAA 
    ACTACACCCA AAATCCAACG TCTACCACCT AGTAGTATTC AAATTCCCCC TCCGAAACCT 
    ATGCAGAAAT TCCCAGCTAA TGCGGCATCT TCTGAATCGC CCCCCAATGC GACTGGAAAC 
    TTTTTACCTT CGGGTCCTGT ACCTGCAAAT GAACCTATGT TAAAACGAGA ATCCGTGGAT 
    TTGATAAAAA TACGACAACT TGCAATTTTG TTTCAACAGC GTGCCTCTCA ATTAAAAGCT 
    AGAGGTGCTA CACGGGAACA AATTACTGAA ATTCTGAATC GTCAAGCAAT AGCCGCTGGT 
    ACTGATTTGG CAACAGTAAT GACGGTTGCA CGAAATCTTC ATTTTCAACA ACTCCAAATG 
    AGACAGCAGC AACAACAGCA GCAAATGAAG GCTGAAAGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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