ORF » Species Summary » Schizosaccharomyces pombe » trm12 cDNA ORF clone

trm12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following trm12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trm12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103636 NM_001018464.2
Latest version!		 Schizosaccharomyces pombe putative tRNA methyltransferase Trm12 (trm12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    trm12 ( NM_001018464.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593066.1 Schizosaccharomyces pombe putative tRNA methyltransferase Trm12 (trm12), mRNA.
    Price & Availability↑
    CloneID OSc103636
    Clone ID Related Accession (Same CDS sequence) NM_001018464.2
    Accession Version NM_001018464.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative tRNA methyltransferase Trm12
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018464.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATTTCTT TTCTAGTACA AAAGTCTGAA GCAAAGTACT GGAAGGATGT TGTTCAAAAT 
    AAAAATAAGT TTCTTAAAAA TATTGGGATC ATTTCGGGAC CTAAATTAGT TGACACCTAC 
    AGCAATCAAT TTGAAGCAGA TTTTCTCAAA AAGTGCGTTC TTATTCCAAC TTCTTATAAC 
    AAAGAAGACC TTCCTAAAGA TATATTAGAT TGCCCAAGAA TTTATTTAAT CGAAGAGGAG 
    GTAAAATCTG TTCAAACAGA TGAAAAATTG CAGATTAAAG TTCTTCGCTA TGTATCTCAA 
    ATCCTTCACA CAACTGTGAG TGAAGAACAA TATCCCGTTC CATCTAGGTT TGTCATTTAT 
    CCTCCGTTAG CTCTATTATC TTTAAACAGT TTTGGCACTA AAGAATGGCA TCTTTTTTTT 
    CAGTCTTATG CAAACGTCGA TGTCAAAGCT GGTTTATTTG ATATTTTTGC TAGAGAATGG 
    AACGTATCAC ATATTGCTAT TAATGAACCT ATTCCTGTGG GGGATGTAAT GAGAAGACCA 
    TTGTCATTGA AACCGCTATA CGGTGACTTT GGAGTGCTAA TTGATGGCAA CCCCTCTGAA 
    CAGGATTTTC AAAAAGCATT TTGGGTAAGT TGTCGTCAAA ACGGTATTTA TCAGACATGG 
    GCTCCTCTTT ATACGATGTT CTCTCGTGGT AATTCGATTG AAAAGGCCAG AGTACTTAAT 
    TTTAGTTACA TTAAAGACGA AATTATTGCA GATCTTTACG CTGGTATAGG GTACTTTACA 
    TTTTCTTACG TTAAAGCGGG AGCTTCAACA GTTTTTTGCT GGGAAATTAA TCCATGGAGT 
    GTTGAAGCGT TAAGGAGAGC TGCTTTGAAA AATGGCTGGT CTATTTATGT AGTTCGAAAT 
    GGAGAAAACT ATGAATTTAA AGTAGGAACT CATCGTATTG TAGTTTTTTT GGAAAGCAAC 
    GTTTATGCAG CTGAAAGGTT CTCCAAAATG AATATTTCTG CTCGACATAT AAATTTGGGA 
    ATGCTCCCCT CTAGTGAAAA ATCCTGGTCA ACCGCTACTT CAATTTTAAA GCGAGAATCA 
    CATTCCTTTA TACATGTTCA TGAGAACGTA AAAGATGAAG ATATCGAAAC ATACTCTTCT 
    GAAGTGAATT CATGTTTTTC AAAAATGTTG GCTAAAAATA CAGTTTGTGT TACAAATTGT 
    GTCAAATCAT TTTCACCACG AGTGTCACAT ATCGTTTACG ATATAGAGAC GGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593066.1
    CDS99..1355
    Translation

    Target ORF information:

    RefSeq Version NM_001018464.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative tRNA methyltransferase Trm12 (trm12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018464.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATTTCTT TTCTAGTACA AAAGTCTGAA GCAAAGTACT GGAAGGATGT TGTTCAAAAT 
    AAAAATAAGT TTCTTAAAAA TATTGGGATC ATTTCGGGAC CTAAATTAGT TGACACCTAC 
    AGCAATCAAT TTGAAGCAGA TTTTCTCAAA AAGTGCGTTC TTATTCCAAC TTCTTATAAC 
    AAAGAAGACC TTCCTAAAGA TATATTAGAT TGCCCAAGAA TTTATTTAAT CGAAGAGGAG 
    GTAAAATCTG TTCAAACAGA TGAAAAATTG CAGATTAAAG TTCTTCGCTA TGTATCTCAA 
    ATCCTTCACA CAACTGTGAG TGAAGAACAA TATCCCGTTC CATCTAGGTT TGTCATTTAT 
    CCTCCGTTAG CTCTATTATC TTTAAACAGT TTTGGCACTA AAGAATGGCA TCTTTTTTTT 
    CAGTCTTATG CAAACGTCGA TGTCAAAGCT GGTTTATTTG ATATTTTTGC TAGAGAATGG 
    AACGTATCAC ATATTGCTAT TAATGAACCT ATTCCTGTGG GGGATGTAAT GAGAAGACCA 
    TTGTCATTGA AACCGCTATA CGGTGACTTT GGAGTGCTAA TTGATGGCAA CCCCTCTGAA 
    CAGGATTTTC AAAAAGCATT TTGGGTAAGT TGTCGTCAAA ACGGTATTTA TCAGACATGG 
    GCTCCTCTTT ATACGATGTT CTCTCGTGGT AATTCGATTG AAAAGGCCAG AGTACTTAAT 
    TTTAGTTACA TTAAAGACGA AATTATTGCA GATCTTTACG CTGGTATAGG GTACTTTACA 
    TTTTCTTACG TTAAAGCGGG AGCTTCAACA GTTTTTTGCT GGGAAATTAA TCCATGGAGT 
    GTTGAAGCGT TAAGGAGAGC TGCTTTGAAA AATGGCTGGT CTATTTATGT AGTTCGAAAT 
    GGAGAAAACT ATGAATTTAA AGTAGGAACT CATCGTATTG TAGTTTTTTT GGAAAGCAAC 
    GTTTATGCAG CTGAAAGGTT CTCCAAAATG AATATTTCTG CTCGACATAT AAATTTGGGA 
    ATGCTCCCCT CTAGTGAAAA ATCCTGGTCA ACCGCTACTT CAATTTTAAA GCGAGAATCA 
    CATTCCTTTA TACATGTTCA TGAGAACGTA AAAGATGAAG ATATCGAAAC ATACTCTTCT 
    GAAGTGAATT CATGTTTTTC AAAAATGTTG GCTAAAAATA CAGTTTGTGT TACAAATTGT 
    GTCAAATCAT TTTCACCACG AGTGTCACAT ATCGTTTACG ATATAGAGAC GGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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