hsp90 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	hsp90
Entrez Gene ID	2543539
Full Name	Hsp90 chaperone
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019786.2	NP_594365.1	Hsp90 chaperone

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsp83	NP_001261362.1
Arabidopsis thaliana (thale cress)	HSP90.1	NP_200076.1
Mus musculus (house mouse)	Hsp90ab1	NP_032328.2
Gallus gallus (chicken)	HSP90AB1	NP_996842.1
Saccharomyces cerevisiae (baker's yeast)	HSP82	NP_015084.1
Kluyveromyces lactis	KLLA0D12958g	XP_453640.1
Schizosaccharomyces pombe (fission yeast)	hsp90	NP_594365.1
Canis lupus familiaris (dog)	HSP90AB1	XP_532154.4
Bos taurus (cattle)	HSP90AB1	NP_001073105.1
Danio rerio (zebrafish)	hsp90ab1	NP_571385.2
Caenorhabditis elegans (roundworm)	daf-21	NP_506626.1
Neurospora crassa	NCU04142	XP_961298.1
Oryza sativa (rice)	Os08g0500700	NP_001062159.1
Homo sapiens (human)	HSP90AB1	NP_001258898.1
Pan troglodytes (chimpanzee)	HSP90AB1	XP_003311385.1
	HSP90AB1	XP_001099135.2
Rattus norvegicus (Norway rat)	Hsp90ab1	NP_001004082.3
Anopheles gambiae (African malaria mosquito)	HSP83_ANOGA	XP_308800.3
Eremothecium gossypii	AGOS_AFL148C	NP_985402.1
Magnaporthe oryzae (rice blast fungus)	MGG_06759	XP_003709473.1
Xenopus tropicalis (tropical clawed frog)	hsp90ab1	NP_001025655.1
Saccharomyces cerevisiae (baker's yeast)	HSC82	NP_013911.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cochaperones can modify Hsp90 by strengthening of the coupling between ATP hydrolysis and a kinetic step involved in the Hsp90 system resulting in a stronger directionality.
Title: Four-colour FRET reveals directionality in the Hsp90 multicomponent machinery.

The co-chaperones Sti1 and Aha1 bound Hsp90 and modulated its activity
Title: Biochemical characterization and cooperation with co-chaperones of heat shock protein 90 from Schizosaccharomyces pombe.

cochaperone Hsp90 may not be essential for Cdc37 function in S. pombe
Title: Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90.

Analyses of Myo2p, Swo1p, and the UCS domain protein Rng3p establish that Swo1p and Rng3p collaborate in vivo to modulate myosin II function.
Title: Hsp90 protein in fission yeast Swo1p and UCS protein Rng3p facilitate myosin II assembly and function.

The following hsp90 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hsp90 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103069	NM_001019786.2 Latest version!	Schizosaccharomyces pombe Hsp90 chaperone (hsp90), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hsp90 ( NM_001019786.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594365.1 Schizosaccharomyces pombe Hsp90 chaperone (hsp90), mRNA.

Price & Availability↑

CloneID	OSc103069
Clone ID Related Accession (Same CDS sequence)	NM_001019786.2
Accession Version	NM_001019786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Hsp90 chaperone
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019786.1.

ATGTCGAACA CAGAAACTTT CAAGTTTGAG GCTGAAATCA GCCAGTTGAT GTCTTTAATC 
ATCAACACTG TTTATTCTAA CAAGGAAATT TTCCTTCGTG AGCTTATTTC CAACGCTTCC 
GATGCCTTGG ACAAAATTCG TTATCAAAGC CTTTCCGATC CTCATGCCTT AGACGCTGAG 
AAGGATCTTT TCATTCGCAT TACTCCTGAC AAGGAGAACA AAATCCTTAG CATTCGCGAT 
ACCGGTATTG GTATGACCAA GAATGACCTT ATCAACAACC TTGGTGTTAT CGCCAAGTCT 
GGTACTAAGC AGTTTATGGA AGCTGCTGCT TCTGGTGCTG ATATCTCCAT GATTGGTCAA 
TTCGGTGTTG GTTTCTACTC TGCATACTTG GTTGCCGACA AGGTCCAAGT CGTAAGTAAG 
CATAATGACG ATGAGCAATA CATCTGGGAA TCCTCTGCTG GTGGTAGCTT CACCGTCACT 
TTGGACACCG ATGGACCTCG CTTGTTACGT GGTACCGAGA TTCGTCTCTT CATGAAAGAA 
GACCAACTTC AATACCTCGA GGAAAAGACT ATCAAAGATA CCGTCAAGAA ACATAGTGAG 
TTCATCTCTT ACCCCATTCA ATTAGTTGTT ACCCGTGAGG TTGAGAAGGA GGTCCCTGAA 
GAAGAAGAGA CCGAAGAAGT TAAGAATGAA GAAGACGATA AGGCTCCTAA GATTGAGGAG 
GTTGATGATG AATCTGAGAA GAAGGAGAAG AAGACCAAGA AGGTCAAGGA GACCACCACT 
GAGACTGAGG AGTTGAACAA GACCAAGCCC ATTTGGACTC GCAACCCCAG TGAAGTTACC 
AAGGAAGAGT ATGCCTCTTT CTACAAGTCT TTGACTAACG ACTGGGAAGA CCATTTGGCC 
GTCAAGCACT TCAGCGTTGA AGGTCAATTG GAATTCCGTG CCATTCTTTT CGTCCCCCGC 
CGCGCTCCTA TGGACCTTTT TGAGGCCAAG CGTAAGAAGA ACAACATCAA GCTCTATGTT 
CGCCGTGTTT TCATTACCGA CGACTGTGAA GAGTTGATTC CTGAATGGTT GGGTTTCATT 
AAGGGTGTTG TTGATTCTGA GGACTTGCCC TTGAACTTGT CTCGTGAGAT GCTTCAACAA 
AACAAGATCA TGAAGGTTAT TCGTAAGAAC CTTGTCCGTC GTTGTCTTGA TATGTTCAAC 
GAGATTGCCG AGGACAAGGA GAACTTCAAG ACTTTCTACG ATGCTTTCAG CAAGAATTTG 
AAGCTTGGTA TCCATGAGGA TGCTGCTAAC CGCCCTGCTT TAGCCAAGCT TTTGCGTTAC 
AACTCCCTTA ACTCCCCTGA TGATCTTATC AGTCTTGAGG ACTACATCAC TAAGATGCCC 
GAACATCAAA AGAACATTTA TTTCATCACT GGTGAATCTA AGCAAGCCGT TGAGAACTCT 
CCCTTCTTGG AAATCTTCCG TGCCAAGAAG TTTGATGTTC TCTTCATGGT TGACCCCATT 
GATGAGTACG CTGTCACTCA ACTCAAGGAA TTCGAGGGCA AGAAATTGGT TAACATTACT 
AAGGATGGTC TTGAGTTGGA AGAGACTGAC GAGGAGAAGG CTGCTCGCGA GAAGCTCGAA 
AAAGAGTATG AGGAGTTTGC TAAGCAACTT AAGACCATTT TGGGTGACAA GGTTGAAAAG 
GTTGTTGTTT CTAACAAGAT TGTTGGTTCT CCTTGCTTGT TAACTACCGG TCAATATGGT 
TGGTCTGCTA ACATGGAACG TATCATGAAG GCTCAAGCCC TTCGTGATAC TTCTATGAGT 
GCCTATATGT CTAGCCGCAA GACTTTTGAG ATTAACCCCA AGTCTCCCAT CATTGCTGAG 
CTCAAGAAGA AGGTTGAGGA GAATGGTGCC GAAGACCGCT CTGTGAAGGA TCTTGCTACT 
ATTTTGTATG AGACCGCCTT GTTGTCCTCT GGTTTCACTC TTGATGACCC TAGTGCTTAT 
GCTCAACGTA TCAACCGTCT TATTTCTCTT GGTCTTAGCA TTGACGAAGA GGAGGAGGCT 
CCTATTGAGG AAATTTCTAC CGAATCCGTC GCTGCTGAAA ACAATGCTGA GAGCAAGATG 
GAGGAAGTCG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594365.1
CDS	270..2384
Translation	MSNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQSLSDPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAAASGADISMIGQFGVGFYSAYLVADKVQVVSKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEIRLFMKEDQLQYLEEKTIKDTVKKHSEFISYPIQLVVTREVEKEVPEEEETEEVKNEEDDKAPKIEEVDDESEKKEKKTKKVKETTTETEELNKTKPIWTRNPSEVTKEEYASFYKSLTNDWEDHLAVKHFSVEGQLEFRAILFVPRRAPMDLFEAKRKKNNIKLYVRRVFITDDCEELIPEWLGFIKGVVDSEDLPLNLSREMLQQNKIMKVIRKNLVRRCLDMFNEIAEDKENFKTFYDAFSKNLKLGIHEDAANRPALAKLLRYNSLNSPDDLISLEDYITKMPEHQKNIYFITGESKQAVENSPFLEIFRAKKFDVLFMVDPIDEYAVTQLKEFEGKKLVNITKDGLELEETDEEKAAREKLEKEYEEFAKQLKTILGDKVEKVVVSNKIVGSPCLLTTGQYGWSANMERIMKAQALRDTSMSAYMSSRKTFEINPKSPIIAELKKKVEENGAEDRSVKDLATILYETALLSSGFTLDDPSAYAQRINRLISLGLSIDEEEEAPIEEISTESVAAENNAESKMEEVD

Target ORF information:

RefSeq Version	NM_001019786.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Hsp90 chaperone (hsp90), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019786.2

ORF Insert Sequence:

ATGTCGAACA CAGAAACTTT CAAGTTTGAG GCTGAAATCA GCCAGTTGAT GTCTTTAATC 
ATCAACACTG TTTATTCTAA CAAGGAAATT TTCCTTCGTG AGCTTATTTC CAACGCTTCC 
GATGCCTTGG ACAAAATTCG TTATCAAAGC CTTTCCGATC CTCATGCCTT AGACGCTGAG 
AAGGATCTTT TCATTCGCAT TACTCCTGAC AAGGAGAACA AAATCCTTAG CATTCGCGAT 
ACCGGTATTG GTATGACCAA GAATGACCTT ATCAACAACC TTGGTGTTAT CGCCAAGTCT 
GGTACTAAGC AGTTTATGGA AGCTGCTGCT TCTGGTGCTG ATATCTCCAT GATTGGTCAA 
TTCGGTGTTG GTTTCTACTC TGCATACTTG GTTGCCGACA AGGTCCAAGT CGTAAGTAAG 
CATAATGACG ATGAGCAATA CATCTGGGAA TCCTCTGCTG GTGGTAGCTT CACCGTCACT 
TTGGACACCG ATGGACCTCG CTTGTTACGT GGTACCGAGA TTCGTCTCTT CATGAAAGAA 
GACCAACTTC AATACCTCGA GGAAAAGACT ATCAAAGATA CCGTCAAGAA ACATAGTGAG 
TTCATCTCTT ACCCCATTCA ATTAGTTGTT ACCCGTGAGG TTGAGAAGGA GGTCCCTGAA 
GAAGAAGAGA CCGAAGAAGT TAAGAATGAA GAAGACGATA AGGCTCCTAA GATTGAGGAG 
GTTGATGATG AATCTGAGAA GAAGGAGAAG AAGACCAAGA AGGTCAAGGA GACCACCACT 
GAGACTGAGG AGTTGAACAA GACCAAGCCC ATTTGGACTC GCAACCCCAG TGAAGTTACC 
AAGGAAGAGT ATGCCTCTTT CTACAAGTCT TTGACTAACG ACTGGGAAGA CCATTTGGCC 
GTCAAGCACT TCAGCGTTGA AGGTCAATTG GAATTCCGTG CCATTCTTTT CGTCCCCCGC 
CGCGCTCCTA TGGACCTTTT TGAGGCCAAG CGTAAGAAGA ACAACATCAA GCTCTATGTT 
CGCCGTGTTT TCATTACCGA CGACTGTGAA GAGTTGATTC CTGAATGGTT GGGTTTCATT 
AAGGGTGTTG TTGATTCTGA GGACTTGCCC TTGAACTTGT CTCGTGAGAT GCTTCAACAA 
AACAAGATCA TGAAGGTTAT TCGTAAGAAC CTTGTCCGTC GTTGTCTTGA TATGTTCAAC 
GAGATTGCCG AGGACAAGGA GAACTTCAAG ACTTTCTACG ATGCTTTCAG CAAGAATTTG 
AAGCTTGGTA TCCATGAGGA TGCTGCTAAC CGCCCTGCTT TAGCCAAGCT TTTGCGTTAC 
AACTCCCTTA ACTCCCCTGA TGATCTTATC AGTCTTGAGG ACTACATCAC TAAGATGCCC 
GAACATCAAA AGAACATTTA TTTCATCACT GGTGAATCTA AGCAAGCCGT TGAGAACTCT 
CCCTTCTTGG AAATCTTCCG TGCCAAGAAG TTTGATGTTC TCTTCATGGT TGACCCCATT 
GATGAGTACG CTGTCACTCA ACTCAAGGAA TTCGAGGGCA AGAAATTGGT TAACATTACT 
AAGGATGGTC TTGAGTTGGA AGAGACTGAC GAGGAGAAGG CTGCTCGCGA GAAGCTCGAA 
AAAGAGTATG AGGAGTTTGC TAAGCAACTT AAGACCATTT TGGGTGACAA GGTTGAAAAG 
GTTGTTGTTT CTAACAAGAT TGTTGGTTCT CCTTGCTTGT TAACTACCGG TCAATATGGT 
TGGTCTGCTA ACATGGAACG TATCATGAAG GCTCAAGCCC TTCGTGATAC TTCTATGAGT 
GCCTATATGT CTAGCCGCAA GACTTTTGAG ATTAACCCCA AGTCTCCCAT CATTGCTGAG 
CTCAAGAAGA AGGTTGAGGA GAATGGTGCC GAAGACCGCT CTGTGAAGGA TCTTGCTACT 
ATTTTGTATG AGACCGCCTT GTTGTCCTCT GGTTTCACTC TTGATGACCC TAGTGCTTAT 
GCTCAACGTA TCAACCGTCT TATTTCTCTT GGTCTTAGCA TTGACGAAGA GGAGGAGGCT 
CCTATTGAGG AAATTTCTAC CGAATCCGTC GCTGCTGAAA ACAATGCTGA GAGCAAGATG 
GAGGAAGTCG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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hsp90 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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