klp2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	klp2
Entrez Gene ID	2543360
Full Name	kinesin-like protein Klp2
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018891.2	NP_593458.1	kinesin-like protein Klp2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B10956g	XP_452016.1
Eremothecium gossypii	AGOS_AGR253W	NP_986919.1
Saccharomyces cerevisiae (baker's yeast)	KAR3	NP_015467.1
Oryza sativa (rice)	Os03g0862200	NP_001051987.2
Oryza sativa (rice)	Os07g0105700	NP_001058699.1
Schizosaccharomyces pombe (fission yeast)	klp2	NP_593458.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Klp2-dependent nuclear congression proceeds at constant speed, whereas dynein accumulation results in an increase of nuclear velocity over time.
Title: Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.

We observe SIN-dependent disappearance of Klp2 from MTs in anaphase, and we find that this is mediated by direct phosphorylation of Klp2 by the SIN kinase Sid2, which abrogates loading of Klp2 onto MTs by inhibiting its interaction with Mal3
Title: The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning.

Data indicate that Klp2 is required for the proper organization of S. pombe interphase microtubules into bipolar arrays of parallel-overlapped microtubules, suggesting that kinesin-14-dependent microtubule sorting may have wide biological importance.
Title: The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism.

Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.
Title: Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.

The polarized distribution of interphase microtubules is mediated by the kinesin Klp2, which helps microtubules to become properly organized with plus ends mostly oriented toward the cell ends and minus ends toward the cell center. [klp2]
Title: The kinesin Klp2 mediates polarization of interphase microtubules in fission yeast.

The following klp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103332	NM_001018891.2 Latest version!	Schizosaccharomyces pombe kinesin-like protein Klp2 (klp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klp2 ( NM_001018891.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593458.1 Schizosaccharomyces pombe kinesin-like protein Klp2 (klp2), mRNA.

Price & Availability↑

CloneID	OSc103332
Clone ID Related Accession (Same CDS sequence)	NM_001018891.2
Accession Version	NM_001018891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	kinesin-like protein Klp2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018891.1.

ATGGAAGAAG AAGGACATAA AAGTTTAACT TCGCATCTAC CTCAGTCCTC GAGTTCTTTG 
TCGCAAAGTC GAGAAATTGC TAAAGAATTT ACGTCTAATA TACCTCCTCC GACTATTAAA 
ACTAATTCAT CTTCATCAAA TATTCTGAAA CCTCGGTTAT CATTGCAAAA TGAAGTTAAC 
CAACTGAAAC CTGCTAAATT TCCCAGTAAG ATGTTACCAC CAGGAAGCTT AGCCTCGGTT 
AAATCTTCTA GTCTTGCAAA AAAAGCCAGG CCTTTTACCG CGTCCTCTAA TCCCAGGATG 
CCCAAGTCTG CACATCCCAT TTCAAGTCGG TCAGTGTCTG CTTCTTCTCA TTTTGGTAGA 
CCTGCCAGTG CTGTTTCATC CAGTTTAAAT AGTAGCGATG ATGTGAGAAG TATGTCAGAT 
GAAAGTATGG AATCTTATAA TGATGAAAAG TCTGTTAATG CCAGCGCCTT AAGGACTACA 
GAAGACCGGT TGAGGAGTAT GGAAATGGCT TATGCTCAAT TATCGGCTAA AGTTATTCCT 
TCACCCTCAA AAAGACCAGC TAATTACAAA TTTTACGAAC AGAGAATTGC TATGCTTGAA 
GAATCATTAG AAGTCGAGAG GAGTCGTACA TCGGAGTTAC AAGAACAATT TTCAGTGGCT 
CTGAGAGAAA AAGCTGAGGC GGAGGCTAAT AAAATCGTTT CTCAAAAGGG TATGGAGAGT 
TTGGAGATAA TGTTAAACTC AATGAAAAGT GAAAATCATC AGCGCATGGC CATGTTGGAA 
GAGAACCATG CTCGGGTGAT GGAGACAGCT GAGCTTCAGC ATCAAGCAGA ATTACAGGAT 
TTTGCTTCCA ATATTGAACA AAAAGCAAAT AGTTTAATCA TGGAGTACAA AAATGAATTG 
CAGTCTGCCG AAGAACACTT TTCACACAAG ATTAAAGAGC TTACTTCAGA AAATGAACTC 
AAAATTTCCA GACTACAAGA GGAAAAGGAT TCTTTATTAA AGAAAGTTCA GGAGGGCGCT 
TCGCTGGCTA TGCAACGTGT CCAAAATAAG CATGATCTAG AAAAAAAAAG ACTGCAATCT 
GCAATTCAAC CGCTTCAGGA AGAAAATAAT TCTTTGAAAC AGCAGATTGA ACAATTACAA 
AGAGAACTTG CTTCTGAAAC AGTTGTTAAG GAAAATCTGA AATCTTCGTT GGACCAACAA 
AGTGCCAACG TTCAGAAGCT TGAATCTACA AATCGGGCTT TAGAAAGCAC TATCAAAACA 
CTTGAAGAAG ATGTTTATAC TATGAAAAAT AAGATTATAG AACTAGAAGG CATTTTAAAA 
TCTGCCAATG TTGAGAGAGA CGGTTTAGTT GAGAAACTCA TAGCTGAGGA AACTCTGAGA 
AGAAAACTCC ATAATACCAT TCAAGAACTT AAAGGTAACA TACGCGTTTT TTGTCGAGTG 
CGACCTCCCC TTGGCGATGG AGAATCAGCA CAAATAGCTT TTCCTGATCA AAATTCCGAA 
GCTAGTACTA TAGAGATTGT TGCTCAAGCC CCAGGGTCTT CGTTAACAGG GAACGGGATA 
AAACAGTATG CCTTTAATTT CGATCGTGTA TTTTCCCCTG AAACGACTAA TGAGGATGTA 
TTTAATGAAC TGTCACAGTT AATTCAAAGT GCTATGGACG GATATAATGT TTGTATATTC 
GCTTACGGTC AAACAGGTTC AGGCAAGACG CATACGATGT CTTCAAATAC AGGAATGATC 
CCTTCATCTG TAAGAATGAT ATACAATCGC TCTACTTCTT TGAAAGAACG AGGTTGGGAA 
TATCGGATGG AAGGACAATT TCTAGAAATA TACAATGAAA CCATTATTGA CCTTTTAGCC 
TCCGGAAATG AAGAAGAGAA GGGAAAGAAA AAACTGGAGA TATATCATGA CACGAAGGCC 
GGAAGGACGA CTATTACCAA TATAACCAGC GAACCGTTAG ACACACCTGA GCAGGTAACC 
TGGTTGTTAG ATCAAGCTTC GAAGAACCGT TCTGTTGCGG CTACCAATGC TAATGAACAT 
TCCTCAAGAT CGCATTCAGT TTTTATGCTA CACTTGAATG GAAGCAATTC TACGACAGGT 
GAAACATGCA GAAGTACGCT TAATTTGATT GATCTGGCTG GATCCGAAAG ACTTTCGAGT 
AGCCAAAGTG TGGGTGAACG TTTGAAGGAA ACGCAGGCAA TCAACAAAAG TTTAAGTTGT 
TTAGGAGATG TAATACATGC TTTGGGAAGT GGAAAGGAAG GAACGTACAT TCCGTATCGA 
AATTCCAAGC TGACTAATTT GTTACAATAT TCGTTGGGAG GAAATAGTAA AACTCTCATG 
TTTGTCAATA TTTCCCCACT AAAACAGCAT GTTCCCGAAA CATTATGCAG TTTGAGATTT 
GCAACAAAGG TAAATAATAC TCAAATTGGC ACAGCACGCA AAGTCACAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593458.1
CDS	97..2550
Translation	MEEEGHKSLTSHLPQSSSSLSQSREIAKEFTSNIPPPTIKTNSSSSNILKPRLSLQNEVNQLKPAKFPSKMLPPGSLASVKSSSLAKKARPFTASSNPRMPKSAHPISSRSVSASSHFGRPASAVSSSLNSSDDVRSMSDESMESYNDEKSVNASALRTTEDRLRSMEMAYAQLSAKVIPSPSKRPANYKFYEQRIAMLEESLEVERSRTSELQEQFSVALREKAEAEANKIVSQKGMESLEIMLNSMKSENHQRMAMLEENHARVMETAELQHQAELQDFASNIEQKANSLIMEYKNELQSAEEHFSHKIKELTSENELKISRLQEEKDSLLKKVQEGASLAMQRVQNKHDLEKKRLQSAIQPLQEENNSLKQQIEQLQRELASETVVKENLKSSLDQQSANVQKLESTNRALESTIKTLEEDVYTMKNKIIELEGILKSANVERDGLVEKLIAEETLRRKLHNTIQELKGNIRVFCRVRPPLGDGESAQIAFPDQNSEASTIEIVAQAPGSSLTGNGIKQYAFNFDRVFSPETTNEDVFNELSQLIQSAMDGYNVCIFAYGQTGSGKTHTMSSNTGMIPSSVRMIYNRSTSLKERGWEYRMEGQFLEIYNETIIDLLASGNEEEKGKKKLEIYHDTKAGRTTITNITSEPLDTPEQVTWLLDQASKNRSVAATNANEHSSRSHSVFMLHLNGSNSTTGETCRSTLNLIDLAGSERLSSSQSVGERLKETQAINKSLSCLGDVIHALGSGKEGTYIPYRNSKLTNLLQYSLGGNSKTLMFVNISPLKQHVPETLCSLRFATKVNNTQIGTARKVTK

Target ORF information:

RefSeq Version	NM_001018891.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe kinesin-like protein Klp2 (klp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018891.2

ORF Insert Sequence:

ATGGAAGAAG AAGGACATAA AAGTTTAACT TCGCATCTAC CTCAGTCCTC GAGTTCTTTG 
TCGCAAAGTC GAGAAATTGC TAAAGAATTT ACGTCTAATA TACCTCCTCC GACTATTAAA 
ACTAATTCAT CTTCATCAAA TATTCTGAAA CCTCGGTTAT CATTGCAAAA TGAAGTTAAC 
CAACTGAAAC CTGCTAAATT TCCCAGTAAG ATGTTACCAC CAGGAAGCTT AGCCTCGGTT 
AAATCTTCTA GTCTTGCAAA AAAAGCCAGG CCTTTTACCG CGTCCTCTAA TCCCAGGATG 
CCCAAGTCTG CACATCCCAT TTCAAGTCGG TCAGTGTCTG CTTCTTCTCA TTTTGGTAGA 
CCTGCCAGTG CTGTTTCATC CAGTTTAAAT AGTAGCGATG ATGTGAGAAG TATGTCAGAT 
GAAAGTATGG AATCTTATAA TGATGAAAAG TCTGTTAATG CCAGCGCCTT AAGGACTACA 
GAAGACCGGT TGAGGAGTAT GGAAATGGCT TATGCTCAAT TATCGGCTAA AGTTATTCCT 
TCACCCTCAA AAAGACCAGC TAATTACAAA TTTTACGAAC AGAGAATTGC TATGCTTGAA 
GAATCATTAG AAGTCGAGAG GAGTCGTACA TCGGAGTTAC AAGAACAATT TTCAGTGGCT 
CTGAGAGAAA AAGCTGAGGC GGAGGCTAAT AAAATCGTTT CTCAAAAGGG TATGGAGAGT 
TTGGAGATAA TGTTAAACTC AATGAAAAGT GAAAATCATC AGCGCATGGC CATGTTGGAA 
GAGAACCATG CTCGGGTGAT GGAGACAGCT GAGCTTCAGC ATCAAGCAGA ATTACAGGAT 
TTTGCTTCCA ATATTGAACA AAAAGCAAAT AGTTTAATCA TGGAGTACAA AAATGAATTG 
CAGTCTGCCG AAGAACACTT TTCACACAAG ATTAAAGAGC TTACTTCAGA AAATGAACTC 
AAAATTTCCA GACTACAAGA GGAAAAGGAT TCTTTATTAA AGAAAGTTCA GGAGGGCGCT 
TCGCTGGCTA TGCAACGTGT CCAAAATAAG CATGATCTAG AAAAAAAAAG ACTGCAATCT 
GCAATTCAAC CGCTTCAGGA AGAAAATAAT TCTTTGAAAC AGCAGATTGA ACAATTACAA 
AGAGAACTTG CTTCTGAAAC AGTTGTTAAG GAAAATCTGA AATCTTCGTT GGACCAACAA 
AGTGCCAACG TTCAGAAGCT TGAATCTACA AATCGGGCTT TAGAAAGCAC TATCAAAACA 
CTTGAAGAAG ATGTTTATAC TATGAAAAAT AAGATTATAG AACTAGAAGG CATTTTAAAA 
TCTGCCAATG TTGAGAGAGA CGGTTTAGTT GAGAAACTCA TAGCTGAGGA AACTCTGAGA 
AGAAAACTCC ATAATACCAT TCAAGAACTT AAAGGTAACA TACGCGTTTT TTGTCGAGTG 
CGACCTCCCC TTGGCGATGG AGAATCAGCA CAAATAGCTT TTCCTGATCA AAATTCCGAA 
GCTAGTACTA TAGAGATTGT TGCTCAAGCC CCAGGGTCTT CGTTAACAGG GAACGGGATA 
AAACAGTATG CCTTTAATTT CGATCGTGTA TTTTCCCCTG AAACGACTAA TGAGGATGTA 
TTTAATGAAC TGTCACAGTT AATTCAAAGT GCTATGGACG GATATAATGT TTGTATATTC 
GCTTACGGTC AAACAGGTTC AGGCAAGACG CATACGATGT CTTCAAATAC AGGAATGATC 
CCTTCATCTG TAAGAATGAT ATACAATCGC TCTACTTCTT TGAAAGAACG AGGTTGGGAA 
TATCGGATGG AAGGACAATT TCTAGAAATA TACAATGAAA CCATTATTGA CCTTTTAGCC 
TCCGGAAATG AAGAAGAGAA GGGAAAGAAA AAACTGGAGA TATATCATGA CACGAAGGCC 
GGAAGGACGA CTATTACCAA TATAACCAGC GAACCGTTAG ACACACCTGA GCAGGTAACC 
TGGTTGTTAG ATCAAGCTTC GAAGAACCGT TCTGTTGCGG CTACCAATGC TAATGAACAT 
TCCTCAAGAT CGCATTCAGT TTTTATGCTA CACTTGAATG GAAGCAATTC TACGACAGGT 
GAAACATGCA GAAGTACGCT TAATTTGATT GATCTGGCTG GATCCGAAAG ACTTTCGAGT 
AGCCAAAGTG TGGGTGAACG TTTGAAGGAA ACGCAGGCAA TCAACAAAAG TTTAAGTTGT 
TTAGGAGATG TAATACATGC TTTGGGAAGT GGAAAGGAAG GAACGTACAT TCCGTATCGA 
AATTCCAAGC TGACTAATTT GTTACAATAT TCGTTGGGAG GAAATAGTAA AACTCTCATG 
TTTGTCAATA TTTCCCCACT AAAACAGCAT GTTCCCGAAA CATTATGCAG TTTGAGATTT 
GCAACAAAGG TAAATAATAC TCAAATTGGC ACAGCACGCA AAGTCACAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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klp2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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