mus7 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mus7
Entrez Gene ID	2543237
Full Name	DNA repair protein Mus7/Mms22
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019187.2	NP_593757.1	DNA repair protein Mus7/Mms22

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

evolutionary conserved Mms1-Mms22 complex is required for replication of damaged DNA in fission yeast.
Title: Mms1-Mms22 complex protects genome integrity in Schizosaccharomyces pombe.

The following mus7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mus7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102282	NM_001019187.2 Latest version!	Schizosaccharomyces pombe DNA repair protein Mus7/Mms22 (mus7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mus7 ( NM_001019187.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593757.1 Schizosaccharomyces pombe DNA repair protein Mus7/Mms22 (mus7), mRNA.

Price & Availability↑

CloneID	OSc102282
Clone ID Related Accession (Same CDS sequence)	NM_001019187.2
Accession Version	NM_001019187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA repair protein Mus7/Mms22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019187.1.

ATGAATAGTT TCCTTTTACT TGACCATGTG TCTGACTCTC AGGATTCTGG AAGTGAATGG 
TCAAATACTC ATGGGTCTTT AATTTCACAA CGTGGAAATG AATACGATGG AATTACGCCG 
GCTACTAAAG AAAAAAATCA AAATAAGCTA GAAATTCTGA ACAATGAAAC CACCATCGGT 
TCTACAAAGT CATCGGTGTC TTCATTGCCC GAATTGAATA GGAATGTTTC TTTTGAAAAA 
AGTGATCAAA ATTTAGCTGA AACAAAGAGG GAAGAAGAAA ACAAACGAAC TGAGGTTTTT 
AAAAAAGATG CCGAACACGA AGCGGTTGTT GTTAAAAGAG ATTTTCGCCC TAGACGTCCT 
GAGCAAACCC GGCCTTATAC TTACGATTTC TTAAGGCATC AAATAGAGTT TAAAAGAATA 
GGCTTGGTAC CCATCACCGT ACCGCATGGT TTTTCTTCTG ATCGATCTAT TACTTCTAAG 
TCCTCTCATA AACCTGTAAA TGTTATAGTT CGTAATCGTG CTTCTTCACG TAAACCACCC 
TTGACCTCTA CTCATCGCTT TAGAAGGTAT GGAGCTGTAA TATCTGATAG CGATGATGAT 
GAGTCTAATA CTGAGCAAGA TCATTCAAAG GAATCTAATC TGAATACAGC TGACAATGAC 
CTCGCATTGT CGTCTACTAT TGAAGGAAAG AAAACCTCAA CTAGCAAAGA GGCTTTAGAA 
TCTGAGTCTT TACTTTCCGA TTCGGATCAG TCTATGACAA ATATCTCATC GAATTCTACT 
GTGAGTGATC TGAATTTGAA AACTTTAAAG AAAAGATTAC GAGGAGTTTT ACCACCATCA 
TTTCTAACTC TTCAAGAAAA AAAAAAATTG GAAAATAGAG GTGTCAAAAA AAAGACATCA 
TTACACAAGA GTGTTATTGA GGGAGAAAAA ATAAAAGGTG TGGCTCGACG GAAGTTGCAT 
CCTAGATCGA CAGCTAAACT ATCTAGTGAA TTAGGAAACG AAATATCGGA TTCTGACAAT 
AGCATTTCTA CGCCAACACC AACTGATGAT TCACGGTTTG ACACCAGCGA GTTTTTAGAT 
TCAATTTCTC GTGATAATGG CTGGTTGAAA GAAGATGTGG TTGATCAATT ATGGCTTCCT 
AAAAGAAGTT TATCCGCTCT TAAAAAATCA AGTTCTTTGA CGTCGGAAAA CCCCTTTCAG 
CTAAATGTAG CAGCCAATGC TGTTTCTACT ATTCCTGTCT ATCGCACAAC TAAAACCAAA 
ATGAAAAAAA ATAGATTTAA ATATGTCGAA GTGGAAAAAT TACCAGACCT TATCCTTGAA 
TCGTATGGGA AAAAGGCGCC AAAGTTTTTG AGGGTGTTTG CTCGAAGTTC ATCTCATATT 
CCAAAAATGA TTAGAAGAAA AAGACAAATG GATTCCAAAA AGTATTTTTC CTTCGACAAA 
GAATCCGATC GCCAAGTTAT TGATCAAGTT CTTTCTGATT GGTATTCAGG TAAACATGAG 
CTTGTTCAAC AATCTCATTC TTACAAAAAG CCTTCAGATT CTAAGTCAGT TGGTGGAAAT 
ATTTTTTCTG TTAATTCTAA AAAGCATAGT GTAAATATTA ACGCTAAAAC AGCAGCAAAT 
AACGGTTTGA GTCATCTTCA AAATTTTAGC GAGGAACTCT TAAAAAAAAG AAAGCTATTT 
TCTTCTCTTT TTTCTAATAA CGTTTCATAC AAAAAATCCA AAAAGTTAAA AAGGACTCAT 
ACAGTACATG ACAAATGCCA AAAAGTTGCC AAATTAGACC ATTACATTAG GGACAATATT 
GAATTAAACT CAAAGGAAAG AGAACATGAT TGTTATGAGG GGACTTTAGC TGTACCCCAA 
GTAAATACTG AGATCCGCAA GTCTTCAAGA AAGCAGAAGG CTCAACGATT TATACGAGAC 
GATTTTGACA CCGTCTTTTT TCAATCCTCT TCAAACCCTA ATTATTTTAC AGATGTAAAT 
CCTTTTTGGA ATATAGGTAT TTGGTCTACT ACATTTAATG TCATAACTTT TAGACCGGGA 
CTTAGTTTGC CCAACAACTC CTTTATAAAA ACCCAAGGGT TAAATTCTAT TCTTCAATTG 
GACATCGTCA CACATCCTTT TAAGTCTGTT TATGCTTTTT CTTGTTTATT CAATATCCAG 
GATGATGTTT TTAAAACATT CGAAAAATTA AAAGACACTT TTGAGACAGT CTTAGAAAAT 
CTTCCATACT TTACAAACTC TGAAACTGTT GACTTGTACA ATCTACTTTC TTTTTGCTCT 
GCGTTTATTC TTCATTCTCA AGTATCAATG GGCTTGGTTA ACTTGGCTTC CTCCTTTTTA 
GAAACCTATG CATTAGTCAA TGATCGTGTT TCTTCTATAT CGGGGTTGAA TAGATCACAA 
TTGGTTGAAA AAATAGCTGT GTTGTTTCAA ACTTTTCAAG TTGTATTTTA TTGTGAATTT 
GAACTTGGAA ATCAGCAAAA CATTAATAAA GTTTCCTGGC TGGCAAGTGA TTTGATTTCA 
AAACTTCTTT CTGCTGGTCA ATCTGGTCTA TTGGAATGTT ATCGGAATTT GAGAATTCAA 
GCTTCAGATA CGACTGTAAT TGACACTCTT TTTTTGGAAT CTTGGTCAAT TTTAAATCAT 
ATTCTTTTTC ATGTATATAA GAAGAAATAT GCTTTGTGGG AACAGGTGAA TTCATTTTTT 
GATCTTCAAA AAAAGGAGTT GTCTATATTA GAAATGGAAA AAATTTGGTA TGTTATAATG 
ACGCTCAATC CCGTTTTCCA AATTGGGCTT AATGGAACGA CTCATTCACC AGGAAACAAT 
AGTTTTTGGC CACTGATAAT CAGAGTTTCA GAAAGTGCAT TTAAAATGCA TAAGGACGGA 
CACAATGTTA AAGTGGTTGA GCGATACCTT CGCACAGTGT TTCTTCGAAT TCATTTTCTC 
ATATCTGAAT GGCGTTGGGA GGATGTGGCT CAAATCCTAT TTTTGATATT CGATTTCTTT 
AGCCATAGAA AGTTTAATGA CCTTTCTTCT GAAATATCTG AAGATACACC AACTGACTTT 
CCTGATTTTG TTAAGTCACT TGACAGACCT CCAAATCTTC ATGTAACTGC TCTTGATACA 
TGTTTTGTAA TTTATTTAAA AGTTATTTTA ATTTCTATAA GCCGTTTGAG GCAAGTAGAT 
GAAAATACAA ATTCAATCAA GAGGATAGTA TCCAGATTAC AGCCCTTACA TTCACGTCAA 
TACACGCGAG AAAGTCCATT CTCAATTAAG GACTTTATGT CTTTGGAACA TACTCACACT 
CTTCTTATTT GTCTTTATTG GGCAGCACCC GAGAATTGTA GGCCCAGTTT AAATCGCATT 
AGGGATATTG TAATCGTCGA TAATTCTCAT CTTAAGGCAC GTTTAATTAG TCTAAAAGCA 
TGGCTTCACC TAATGAAGTA TGTGATAAAA GAGGGAACTG ATTATGAACT CGCTCAGGGA 
ATGGAGTGGT TTAATTCCAT TTTAAAAGTT ACATTTGATG AATATCTTGC TCTCTTTTCA 
AATGGAACTT CTGTTGGTGA GATGCAATTG GCTGAATACT CGAAACATCA ACTTGAAAAT 
GCGCTAATTG TAGCTTTCCA TTCCCTTCAA GATTTAATTC CCAATTCTTC AGTTTATATT 
AGTAGGATAA ACGTTTTGGT GACGGAGCAG TCATGCCGGC GCATTCTTAA AGACTCTCAT 
TTTTTTCCTC CTCGCGTTAC TCTGGAGTGT ATTTTATTTC TAAAAAAGTT TTTGCAGTAT 
CAATCTAATA CAGAGCCACC TAAGGTTACC GTTGTTGGTA GCACTTCGCA TGATAGTCAA 
GATGCTTACT TTGATTCTGA TGTGCTGGAT GATAACACAC TCATTTTAGA ACAAGAAAAG 
TTTGAACGTA AATACGAAGT GGCTCAAATA TTGAGAACAT TTGTTTCACC CTTTTTGTAT 
CAAACGATAT CTTATCTCGT TGGAAATGAC GAGGACAAAG AAAATTATAT TCGTATTTTA 
CTTTTACCAT TAATGGAGTG CATGGCAATT TGTGCTAGTT TTGCTGTAGA AGCGAAAATC 
AATGACTGGA GTTACTATAT CGATTTTGGG TCTGAGTCTT GGGAAAGGAT TCGTAACACT 
CCTTTGAAAA GATCTCTCAG TACCACGTTT TATTCCTTCC TTATATCTTA TAATGATTCT 
TTTATTAAAA AACATGAGGA AAAAGTCTTA ACTGTATGGT TTGAATCACT GGGCGCTTTA 
GATGAGGATC ATGCTGCACA ATTTACGATT TTGTTATTAC AGAAAAACCT AAAAAATCCT 
ATTTTGCTTA ATTTACCGAT ATCCGTCAAA ATTGAGGAAA TTAGTATAAA TTACTTTAAG 
TCGGTTCACT TGAATTTATT AACAGCTGTG TTTTGCAACA TGGCAAAACT GTATGCTGAT 
GCCAAGACAA ATGGTTTCGC GAGCTCTCAA TATTTACAGA GCTTGTTTAT TCATTACCTA 
TCCTCTCTAC TGTCTTCTAT GCAGCATAGT TATGAGACAA ATGGTCATTC TTCTGATACT 
CACTCTTTGT TTGTTATTAA TTCACAACGT GTTGTCGGAG ACATTTTACA GTATTGTAGT 
CAATTTGCAA ATGATCGTAA CCTTCCTGCC CTCAGATATT TTATGGACTC AACTAAGTTC 
CCCCAACCAC CGGAGCGTCT CGAATATACG GCCTTGAGGT TACGAAGTTA TGCGAGAAAA 
ACTCTCACAA ATTCAGCTTC TCAAAATGCT TTGTTTAGTT TCCTAAAAGC AAATTTTGAT 
GTTGCTCTGC TAGAACAAAA GCAAACTGAA ACTTCTAATC TTTTAAGGCT GGCTATGGGA 
TTCCATAATC AAAATAGCTC CAGCAAATGG GATGTCGAGA TTTCTTCTCT TCGGAAATTT 
TGTGTAAAGG AACTTTTATT GAGTTACCTT GGTGAAGGCT CGTTAGCAGC TATGTTTTAT 
TCATTGTTAC TACTGGAGGG TCTAAGCCGA ACTTACAACA GCTTTAGACG TATATATTTG 
CAACCTTACA TTCAACAGTT ACTGTGTGAA GAAATTTCTG TTTTTGTGGC TGATTTAGAG 
AAATATGCTT TATTATATGA AAACAAACGC CCTTTACTTT CTGGGAGAAT ATATTACCTT 
ATTTCTTCAT TTGTGGCTAT AAGCATGACA AACAAAACCA GTGGATTGCC ATCTAGTTTA 
ATATGTAACT TACAGAATTT TTTAGCTCGC ATAGCTCGAG ACTTCCTTCT TGAATTATGT 
TGGAATATTG AAGGGTCTGA GTTTCCCCCT CCTTACAATA CTGTTGATTG TCGTAGCAAA 
TTTGTTAAAA CTATTCAGCA ACATTGCTCA GCGGATTGGT ACGACAACGT TACCAATATT 
GTACATAAGT CTAGGAAGAA AAAACTAGTT TTGCCTTCTC AGGTGGAACA GGAAGAAGAA 
TACTGGTCAG GTTTAATGGA AGTTACTATT CAAATATGGA AACACGATAA AGGTTTTTTA 
GAGCCTGAAT TAGATAGGTT TCTCAATGTA ATTTGCAGTT ATGCACCTGA CATGAGTGGG 
TTACCGACAA AAATCAAATG CTTTTTGGAT AAAATCGGCT ATTCTTGGAT GACTGAAGAG 
AATGATTTTG ATATTGTATT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593757.1
CDS	122..5788
Translation	MNSFLLLDHVSDSQDSGSEWSNTHGSLISQRGNEYDGITPATKEKNQNKLEILNNETTIGSTKSSVSSLPELNRNVSFEKSDQNLAETKREEENKRTEVFKKDAEHEAVVVKRDFRPRRPEQTRPYTYDFLRHQIEFKRIGLVPITVPHGFSSDRSITSKSSHKPVNVIVRNRASSRKPPLTSTHRFRRYGAVISDSDDDESNTEQDHSKESNLNTADNDLALSSTIEGKKTSTSKEALESESLLSDSDQSMTNISSNSTVSDLNLKTLKKRLRGVLPPSFLTLQEKKKLENRGVKKKTSLHKSVIEGEKIKGVARRKLHPRSTAKLSSELGNEISDSDNSISTPTPTDDSRFDTSEFLDSISRDNGWLKEDVVDQLWLPKRSLSALKKSSSLTSENPFQLNVAANAVSTIPVYRTTKTKMKKNRFKYVEVEKLPDLILESYGKKAPKFLRVFARSSSHIPKMIRRKRQMDSKKYFSFDKESDRQVIDQVLSDWYSGKHELVQQSHSYKKPSDSKSVGGNIFSVNSKKHSVNINAKTAANNGLSHLQNFSEELLKKRKLFSSLFSNNVSYKKSKKLKRTHTVHDKCQKVAKLDHYIRDNIELNSKEREHDCYEGTLAVPQVNTEIRKSSRKQKAQRFIRDDFDTVFFQSSSNPNYFTDVNPFWNIGIWSTTFNVITFRPGLSLPNNSFIKTQGLNSILQLDIVTHPFKSVYAFSCLFNIQDDVFKTFEKLKDTFETVLENLPYFTNSETVDLYNLLSFCSAFILHSQVSMGLVNLASSFLETYALVNDRVSSISGLNRSQLVEKIAVLFQTFQVVFYCEFELGNQQNINKVSWLASDLISKLLSAGQSGLLECYRNLRIQASDTTVIDTLFLESWSILNHILFHVYKKKYALWEQVNSFFDLQKKELSILEMEKIWYVIMTLNPVFQIGLNGTTHSPGNNSFWPLIIRVSESAFKMHKDGHNVKVVERYLRTVFLRIHFLISEWRWEDVAQILFLIFDFFSHRKFNDLSSEISEDTPTDFPDFVKSLDRPPNLHVTALDTCFVIYLKVILISISRLRQVDENTNSIKRIVSRLQPLHSRQYTRESPFSIKDFMSLEHTHTLLICLYWAAPENCRPSLNRIRDIVIVDNSHLKARLISLKAWLHLMKYVIKEGTDYELAQGMEWFNSILKVTFDEYLALFSNGTSVGEMQLAEYSKHQLENALIVAFHSLQDLIPNSSVYISRINVLVTEQSCRRILKDSHFFPPRVTLECILFLKKFLQYQSNTEPPKVTVVGSTSHDSQDAYFDSDVLDDNTLILEQEKFERKYEVAQILRTFVSPFLYQTISYLVGNDEDKENYIRILLLPLMECMAICASFAVEAKINDWSYYIDFGSESWERIRNTPLKRSLSTTFYSFLISYNDSFIKKHEEKVLTVWFESLGALDEDHAAQFTILLLQKNLKNPILLNLPISVKIEEISINYFKSVHLNLLTAVFCNMAKLYADAKTNGFASSQYLQSLFIHYLSSLLSSMQHSYETNGHSSDTHSLFVINSQRVVGDILQYCSQFANDRNLPALRYFMDSTKFPQPPERLEYTALRLRSYARKTLTNSASQNALFSFLKANFDVALLEQKQTETSNLLRLAMGFHNQNSSSKWDVEISSLRKFCVKELLLSYLGEGSLAAMFYSLLLLEGLSRTYNSFRRIYLQPYIQQLLCEEISVFVADLEKYALLYENKRPLLSGRIYYLISSFVAISMTNKTSGLPSSLICNLQNFLARIARDFLLELCWNIEGSEFPPPYNTVDCRSKFVKTIQQHCSADWYDNVTNIVHKSRKKKLVLPSQVEQEEEYWSGLMEVTIQIWKHDKGFLEPELDRFLNVICSYAPDMSGLPTKIKCFLDKIGYSWMTEENDFDIVLF

Target ORF information:

RefSeq Version	NM_001019187.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA repair protein Mus7/Mms22 (mus7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019187.2

ORF Insert Sequence:

ATGAATAGTT TCCTTTTACT TGACCATGTG TCTGACTCTC AGGATTCTGG AAGTGAATGG 
TCAAATACTC ATGGGTCTTT AATTTCACAA CGTGGAAATG AATACGATGG AATTACGCCG 
GCTACTAAAG AAAAAAATCA AAATAAGCTA GAAATTCTGA ACAATGAAAC CACCATCGGT 
TCTACAAAGT CATCGGTGTC TTCATTGCCC GAATTGAATA GGAATGTTTC TTTTGAAAAA 
AGTGATCAAA ATTTAGCTGA AACAAAGAGG GAAGAAGAAA ACAAACGAAC TGAGGTTTTT 
AAAAAAGATG CCGAACACGA AGCGGTTGTT GTTAAAAGAG ATTTTCGCCC TAGACGTCCT 
GAGCAAACCC GGCCTTATAC TTACGATTTC TTAAGGCATC AAATAGAGTT TAAAAGAATA 
GGCTTGGTAC CCATCACCGT ACCGCATGGT TTTTCTTCTG ATCGATCTAT TACTTCTAAG 
TCCTCTCATA AACCTGTAAA TGTTATAGTT CGTAATCGTG CTTCTTCACG TAAACCACCC 
TTGACCTCTA CTCATCGCTT TAGAAGGTAT GGAGCTGTAA TATCTGATAG CGATGATGAT 
GAGTCTAATA CTGAGCAAGA TCATTCAAAG GAATCTAATC TGAATACAGC TGACAATGAC 
CTCGCATTGT CGTCTACTAT TGAAGGAAAG AAAACCTCAA CTAGCAAAGA GGCTTTAGAA 
TCTGAGTCTT TACTTTCCGA TTCGGATCAG TCTATGACAA ATATCTCATC GAATTCTACT 
GTGAGTGATC TGAATTTGAA AACTTTAAAG AAAAGATTAC GAGGAGTTTT ACCACCATCA 
TTTCTAACTC TTCAAGAAAA AAAAAAATTG GAAAATAGAG GTGTCAAAAA AAAGACATCA 
TTACACAAGA GTGTTATTGA GGGAGAAAAA ATAAAAGGTG TGGCTCGACG GAAGTTGCAT 
CCTAGATCGA CAGCTAAACT ATCTAGTGAA TTAGGAAACG AAATATCGGA TTCTGACAAT 
AGCATTTCTA CGCCAACACC AACTGATGAT TCACGGTTTG ACACCAGCGA GTTTTTAGAT 
TCAATTTCTC GTGATAATGG CTGGTTGAAA GAAGATGTGG TTGATCAATT ATGGCTTCCT 
AAAAGAAGTT TATCCGCTCT TAAAAAATCA AGTTCTTTGA CGTCGGAAAA CCCCTTTCAG 
CTAAATGTAG CAGCCAATGC TGTTTCTACT ATTCCTGTCT ATCGCACAAC TAAAACCAAA 
ATGAAAAAAA ATAGATTTAA ATATGTCGAA GTGGAAAAAT TACCAGACCT TATCCTTGAA 
TCGTATGGGA AAAAGGCGCC AAAGTTTTTG AGGGTGTTTG CTCGAAGTTC ATCTCATATT 
CCAAAAATGA TTAGAAGAAA AAGACAAATG GATTCCAAAA AGTATTTTTC CTTCGACAAA 
GAATCCGATC GCCAAGTTAT TGATCAAGTT CTTTCTGATT GGTATTCAGG TAAACATGAG 
CTTGTTCAAC AATCTCATTC TTACAAAAAG CCTTCAGATT CTAAGTCAGT TGGTGGAAAT 
ATTTTTTCTG TTAATTCTAA AAAGCATAGT GTAAATATTA ACGCTAAAAC AGCAGCAAAT 
AACGGTTTGA GTCATCTTCA AAATTTTAGC GAGGAACTCT TAAAAAAAAG AAAGCTATTT 
TCTTCTCTTT TTTCTAATAA CGTTTCATAC AAAAAATCCA AAAAGTTAAA AAGGACTCAT 
ACAGTACATG ACAAATGCCA AAAAGTTGCC AAATTAGACC ATTACATTAG GGACAATATT 
GAATTAAACT CAAAGGAAAG AGAACATGAT TGTTATGAGG GGACTTTAGC TGTACCCCAA 
GTAAATACTG AGATCCGCAA GTCTTCAAGA AAGCAGAAGG CTCAACGATT TATACGAGAC 
GATTTTGACA CCGTCTTTTT TCAATCCTCT TCAAACCCTA ATTATTTTAC AGATGTAAAT 
CCTTTTTGGA ATATAGGTAT TTGGTCTACT ACATTTAATG TCATAACTTT TAGACCGGGA 
CTTAGTTTGC CCAACAACTC CTTTATAAAA ACCCAAGGGT TAAATTCTAT TCTTCAATTG 
GACATCGTCA CACATCCTTT TAAGTCTGTT TATGCTTTTT CTTGTTTATT CAATATCCAG 
GATGATGTTT TTAAAACATT CGAAAAATTA AAAGACACTT TTGAGACAGT CTTAGAAAAT 
CTTCCATACT TTACAAACTC TGAAACTGTT GACTTGTACA ATCTACTTTC TTTTTGCTCT 
GCGTTTATTC TTCATTCTCA AGTATCAATG GGCTTGGTTA ACTTGGCTTC CTCCTTTTTA 
GAAACCTATG CATTAGTCAA TGATCGTGTT TCTTCTATAT CGGGGTTGAA TAGATCACAA 
TTGGTTGAAA AAATAGCTGT GTTGTTTCAA ACTTTTCAAG TTGTATTTTA TTGTGAATTT 
GAACTTGGAA ATCAGCAAAA CATTAATAAA GTTTCCTGGC TGGCAAGTGA TTTGATTTCA 
AAACTTCTTT CTGCTGGTCA ATCTGGTCTA TTGGAATGTT ATCGGAATTT GAGAATTCAA 
GCTTCAGATA CGACTGTAAT TGACACTCTT TTTTTGGAAT CTTGGTCAAT TTTAAATCAT 
ATTCTTTTTC ATGTATATAA GAAGAAATAT GCTTTGTGGG AACAGGTGAA TTCATTTTTT 
GATCTTCAAA AAAAGGAGTT GTCTATATTA GAAATGGAAA AAATTTGGTA TGTTATAATG 
ACGCTCAATC CCGTTTTCCA AATTGGGCTT AATGGAACGA CTCATTCACC AGGAAACAAT 
AGTTTTTGGC CACTGATAAT CAGAGTTTCA GAAAGTGCAT TTAAAATGCA TAAGGACGGA 
CACAATGTTA AAGTGGTTGA GCGATACCTT CGCACAGTGT TTCTTCGAAT TCATTTTCTC 
ATATCTGAAT GGCGTTGGGA GGATGTGGCT CAAATCCTAT TTTTGATATT CGATTTCTTT 
AGCCATAGAA AGTTTAATGA CCTTTCTTCT GAAATATCTG AAGATACACC AACTGACTTT 
CCTGATTTTG TTAAGTCACT TGACAGACCT CCAAATCTTC ATGTAACTGC TCTTGATACA 
TGTTTTGTAA TTTATTTAAA AGTTATTTTA ATTTCTATAA GCCGTTTGAG GCAAGTAGAT 
GAAAATACAA ATTCAATCAA GAGGATAGTA TCCAGATTAC AGCCCTTACA TTCACGTCAA 
TACACGCGAG AAAGTCCATT CTCAATTAAG GACTTTATGT CTTTGGAACA TACTCACACT 
CTTCTTATTT GTCTTTATTG GGCAGCACCC GAGAATTGTA GGCCCAGTTT AAATCGCATT 
AGGGATATTG TAATCGTCGA TAATTCTCAT CTTAAGGCAC GTTTAATTAG TCTAAAAGCA 
TGGCTTCACC TAATGAAGTA TGTGATAAAA GAGGGAACTG ATTATGAACT CGCTCAGGGA 
ATGGAGTGGT TTAATTCCAT TTTAAAAGTT ACATTTGATG AATATCTTGC TCTCTTTTCA 
AATGGAACTT CTGTTGGTGA GATGCAATTG GCTGAATACT CGAAACATCA ACTTGAAAAT 
GCGCTAATTG TAGCTTTCCA TTCCCTTCAA GATTTAATTC CCAATTCTTC AGTTTATATT 
AGTAGGATAA ACGTTTTGGT GACGGAGCAG TCATGCCGGC GCATTCTTAA AGACTCTCAT 
TTTTTTCCTC CTCGCGTTAC TCTGGAGTGT ATTTTATTTC TAAAAAAGTT TTTGCAGTAT 
CAATCTAATA CAGAGCCACC TAAGGTTACC GTTGTTGGTA GCACTTCGCA TGATAGTCAA 
GATGCTTACT TTGATTCTGA TGTGCTGGAT GATAACACAC TCATTTTAGA ACAAGAAAAG 
TTTGAACGTA AATACGAAGT GGCTCAAATA TTGAGAACAT TTGTTTCACC CTTTTTGTAT 
CAAACGATAT CTTATCTCGT TGGAAATGAC GAGGACAAAG AAAATTATAT TCGTATTTTA 
CTTTTACCAT TAATGGAGTG CATGGCAATT TGTGCTAGTT TTGCTGTAGA AGCGAAAATC 
AATGACTGGA GTTACTATAT CGATTTTGGG TCTGAGTCTT GGGAAAGGAT TCGTAACACT 
CCTTTGAAAA GATCTCTCAG TACCACGTTT TATTCCTTCC TTATATCTTA TAATGATTCT 
TTTATTAAAA AACATGAGGA AAAAGTCTTA ACTGTATGGT TTGAATCACT GGGCGCTTTA 
GATGAGGATC ATGCTGCACA ATTTACGATT TTGTTATTAC AGAAAAACCT AAAAAATCCT 
ATTTTGCTTA ATTTACCGAT ATCCGTCAAA ATTGAGGAAA TTAGTATAAA TTACTTTAAG 
TCGGTTCACT TGAATTTATT AACAGCTGTG TTTTGCAACA TGGCAAAACT GTATGCTGAT 
GCCAAGACAA ATGGTTTCGC GAGCTCTCAA TATTTACAGA GCTTGTTTAT TCATTACCTA 
TCCTCTCTAC TGTCTTCTAT GCAGCATAGT TATGAGACAA ATGGTCATTC TTCTGATACT 
CACTCTTTGT TTGTTATTAA TTCACAACGT GTTGTCGGAG ACATTTTACA GTATTGTAGT 
CAATTTGCAA ATGATCGTAA CCTTCCTGCC CTCAGATATT TTATGGACTC AACTAAGTTC 
CCCCAACCAC CGGAGCGTCT CGAATATACG GCCTTGAGGT TACGAAGTTA TGCGAGAAAA 
ACTCTCACAA ATTCAGCTTC TCAAAATGCT TTGTTTAGTT TCCTAAAAGC AAATTTTGAT 
GTTGCTCTGC TAGAACAAAA GCAAACTGAA ACTTCTAATC TTTTAAGGCT GGCTATGGGA 
TTCCATAATC AAAATAGCTC CAGCAAATGG GATGTCGAGA TTTCTTCTCT TCGGAAATTT 
TGTGTAAAGG AACTTTTATT GAGTTACCTT GGTGAAGGCT CGTTAGCAGC TATGTTTTAT 
TCATTGTTAC TACTGGAGGG TCTAAGCCGA ACTTACAACA GCTTTAGACG TATATATTTG 
CAACCTTACA TTCAACAGTT ACTGTGTGAA GAAATTTCTG TTTTTGTGGC TGATTTAGAG 
AAATATGCTT TATTATATGA AAACAAACGC CCTTTACTTT CTGGGAGAAT ATATTACCTT 
ATTTCTTCAT TTGTGGCTAT AAGCATGACA AACAAAACCA GTGGATTGCC ATCTAGTTTA 
ATATGTAACT TACAGAATTT TTTAGCTCGC ATAGCTCGAG ACTTCCTTCT TGAATTATGT 
TGGAATATTG AAGGGTCTGA GTTTCCCCCT CCTTACAATA CTGTTGATTG TCGTAGCAAA 
TTTGTTAAAA CTATTCAGCA ACATTGCTCA GCGGATTGGT ACGACAACGT TACCAATATT 
GTACATAAGT CTAGGAAGAA AAAACTAGTT TTGCCTTCTC AGGTGGAACA GGAAGAAGAA 
TACTGGTCAG GTTTAATGGA AGTTACTATT CAAATATGGA AACACGATAA AGGTTTTTTA 
GAGCCTGAAT TAGATAGGTT TCTCAATGTA ATTTGCAGTT ATGCACCTGA CATGAGTGGG 
TTACCGACAA AAATCAAATG CTTTTTGGAT AAAATCGGCT ATTCTTGGAT GACTGAAGAG 
AATGATTTTG ATATTGTATT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mus7 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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