SPAC144.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC144.05
Entrez Gene ID	2542902
Full Name	ATP-dependent DNA helicase (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020095.2	NP_594666.1	ATP-dependent DNA helicase (predicted)
NM_001356173.1	NP_001342916.1	putative ATP-dependent DNA helicase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	IRC20	NP_013348.1
Kluyveromyces lactis	KLLA0F12166g	XP_455630.1
Eremothecium gossypii	AGOS_AAL030C	NP_982512.2
Magnaporthe oryzae (rice blast fungus)	MGG_07487	XP_003711395.1
Schizosaccharomyces pombe (fission yeast)	SPAC144.05	NP_594666.1
Neurospora crassa	NCU03652	XP_960732.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC144.05 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC144.05 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103167	NM_001356173.1 Latest version!	Schizosaccharomyces pombe putative ATP-dependent DNA helicase (SPAC144.05), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSj04088	NM_001020095.2 Latest version!	Schizosaccharomyces pombe 972h- ATP-dependent DNA helicase (predicted) (SPAC144.05), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC144.05 ( NM_001356173.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342916.1 Schizosaccharomyces pombe putative ATP-dependent DNA helicase (SPAC144.05), mRNA.

Price & Availability↑

CloneID	OSc103167
Clone ID Related Accession (Same CDS sequence)	NM_001356173.1
Accession Version	NM_001356173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative ATP-dependent DNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGGACCGAA CTAAGAGGCG AAAAATAGAG AAGGAGGCTT CCTTACTAAA TGAAAGAAAC 
AACCTTTTGG AAAGTGATTT CGCCAATTGC TACCGTGCAT TCCAGCACCA ACTCAAGCTA 
GACCAGCAGA TATGGCGTGG ACCAGAGGAC GAGTTAAATC GGCTAAAGTC TACTATTTAT 
CCTGTCGTTT TTTGGGTTGA TGGATCTGAG AAACTACATG CATATAGTTT TACTCAAAGA 
AAAATTTCAT TGGCCAAAAA CTTGATTCCT TTGTTTTCTG TCGACTTGAA TAAGGATACT 
TACTCTGCTT TAAAAGCACC TCTCAAAATT TGGAGTAAGC TTTGGGAGGA TAATAGGCGG 
CTGAAGAAAA TTATAAAATA CACATCTTAT GTTACTGTAA AAGGATCCGA GTTAATTTTG 
TCGTTTGGCA TTTCCATACT CGACTCTTTT CTTGCCCCTC AAGATGCGAT TCTTTCATCT 
GGATCATCTT CCTCATACAC TGCTTTACTA GACTATACGT TCCTTCCGTC TGAAGATGAG 
GAATATTCTT GTTTAGATAT TAACACTGCA CTATTTTACG ATTGTGCAAG AAAATTGGCA 
AAAAGCCTAC GTTTCGCCAA TGTATCACGC GATCCAAGGC TTTCTTCGGA ACTTTTACCA 
TTTCAAATGC GTGTTTTAGA ATGGATGAAA CGAAGAGAAG AAGAGAAATT CCTAACCTCA 
AATGATTTAC CACCTCTATG GTACCATTGC AAAAGTCTTT TTGATGATCG AATGGTTTAC 
GTTAATCATG TTTACGGCTA TATGACATTT TCGAAAGAAA AAACATATTT GCTTGCGTCC 
GGTGATATAC GTGGAGGCAT TTTAGCCGAT GAAATGGGCA TGGGAAAAAC TTTGGAAGTC 
TTAGGATTAG TTTTGCATCA TCAGTTGCCA ATTTCTTTGA CTGATACTTG TACTTTCGAT 
CAGGTCGTCG GAAAAAATGT AAAGTATTCC AAGGCCACCC TAATAATTAC GCCTTCTACA 
ATTCTCGATC AATGGTTATC TGAGATAGAT TTGCATGTGC CATCATTGAA AGTATTCCAT 
TACCAAGGAA TCCGAAAATC CAATGGTTTG AAATCAGCTA AAATTTTTTT GGATTGTGAT 
ATAGTGGTAA CATCTTATTC TGATTTACGG TTTGAGCTAT TGTACACTGA ATCTCATTCC 
AGGACCTTAA GACATGAAAA GCGACATGTT TCTCCAAAGT CTCCATTGAT TGATGTATGT 
TGGTGGAGAA TTTGTGTTGA TGAAGCTCAG ATGGTTGAAA CTTCACAGAG TAATGTCGCA 
CAAATGATTT ATCGCATACC TCGTGTCAAT TGTTGGACTG TAAGTGGAAC TCCCGTACGT 
AGTGAAGTTG ATGACCTTTT TGGCCTTTTA TTTCTTCTTC GCTACTCCCC TATGTATTTA 
TACAAAAAAC AAGCTTGGAT GCAAATTATA GAGAAGAAAC GTGTTCGTGA ATTTTGCGAT 
CTGTTTGGCT CCCTTGTATG CAGACATTCT AAACAGGACG TTGAAGAAGA ATTAAAATTA 
CCTCCTCAAC ATCGAATCTG CATGACTACA AGACTTAGTG TAGTAGAAGA AACGAACTAC 
CAGGATTTAT TATCAGAGGC GGCCAAGTCT TTGCACTTTT TCAAAGACAG AAATTTGGAT 
TTGTGTGACG AAGAATCTAT GAGGCGTTGG TTGGTTCGTT TACGACAGGC ATGTTGCCAT 
CCGCAGGTTG GATTTGGTAA CAAATCCGCA TTTGGAGGTG GACCCATGAA AAGCATTAAT 
GATGTATTAG TTTTTATGTT GGAACAAACA AACTCTACAT TCTCCTCGTT AAACAGGAAG 
CTTTATTCCG ATAAGATTAT TGTTGGTCAA ATATATGATC ATATTAAAGA CTATAATAAA 
GCATTGGCTA TTTGGTCTGA AGTCAGAATA CCTGTCGAGC TCGCTGTCAA AGAATTAGAA 
AATGTTATTT ACAACTCTAA ATATGAGGAC CATGGTAAAA ATTTACCGAT TAATTATTTT 
GGTTTGGATC ATTTCATTCA TCTGCGTGTT TGGTATGTTC TATTGCATAA AATATACTTT 
TTTATAGCAA GCGCTTATTT TTCTTTAAAA AACGAAAAGT TTGAAAATGA ATTTTATTTG 
CTCGCGCAGG ATTTAAGAAG GAAGATTATG AGTGATGTGA TTATCAAAAC CAGCAAGCAC 
CTTGAAGAGT TTTCAGAAAA ATTTATTCCA AAGAAACTTG TCAAAATACC ACGATTACAG 
AAATCTTACG CAAAAGGTCT CATAACCGGA CATGGTATAA TTGAAGACTA TAATAGACTT 
TATAAAGAGT TGAATGATCA AAAGGAGGTG CTTATAAAAT TTAGAGATCG GCTTATCCAT 
TTGATGAAAC TTCCACTTCT GGATCAAGAA TCGGATCCGA CCGGCGATGA ATACGAAGAA 
TCATTGAATG CGCAATCAGA GATATCCTAT TGTATTGATG TTTACCGTCA AATGCTTTCA 
GATCGAGTAG CGGCAGTCAG CGGTACTATT AATACCTTTG TGTCTCATGA AACAGAACTT 
GAGAAATACA AACTTATAGA GAGTATAAAA AAATCGGAGA AATCGTTAGA CAAGCAGGCT 
GAAGAACGTG ATAAAAAATA TTTGCTTTAC TTTGAGGAAA GGGAAGAAGC AAGGCCAAAA 
GCAGATCAAT ACGGATCATT AATAAATATA GTTTCAAGAC TGTTAGATGC ATCAAATAGA 
TCCACCTCAA GTTTTGAAAC TTCTAAAAAC ATGGAAGAAT ATGAACGGAT TGATGCAATG 
GCAAAGGAGC AATCTAGAAT TTGCCAAAAA TTGGAAAAAG AATTGAGTAT AATTCAGTTG 
ACTTATAATT CAAGGATTGA GTACTATAAA CAGCTGCAGG AAATTTCTGA CTCTTTGATG 
CCTCCTCCCG TTTCAAATAT CAGTTTAAAC AATTACGTTA AAGACGACGA AAAAAAACAA 
AAATTTTTGA ACTCTGTTAT AATTAAAGCT TCGGTGATTT TGGAAAAAGA AATTTCAGAA 
AAGCAGGACG AAGCAAGTCA AACAACAAAC GTAGCAGAAC TCGTCAATCA AAAAATATCC 
GAAATGAATA TACCTGGACA TATCCATCTG CTGCGAGAGC TAGAAGAGGA AAAAAGCAAT 
ACCCAACGTA AAATTGCTCA CTTCGAATCA AGGAGAAGGT ATTTAACAAA CCTATATGAA 
CATATTGTCC TTAAAGCTGA ATCTCATCAG ATTTGTATAA TTTGTCGTGA TATAATTAAG 
CAAGGTTTTA TAACAACTTG TGGCCATTTG TACTGTAGTT TTTGTTTGGA AGCGTGGTTG 
AAGCATAGTT CGTCGTGTCC AATGTGCAAA ACCAAATTGA ACAAAAATAA TGCTTACTAT 
ATTGGCGAAA GTAGGGATAT ATACTCTCGT CAAGAGTTTG TCACTGGTTT CAACAAACGA 
GACGAAAGGC TTGAAATTCT CGACGATGAA GCATACAGGC AAATTTCTAA TATGGAGTTA 
AAGGAGTCAT TTGGATCAAA AATTGACACA ATATCTAAAC ATCTTTTGTA TTTGAAACAT 
AACGAACTTT ATCCTAAAGT TGTTGTATTC TCTCAATGGC TTGATGTTTT GGATGTTTTG 
CACAAGTCAT TTGAAGCGAA TGGTATCGTC TTCATACGTT TTGATGGAAA GTCAAAGAAC 
ACTTGCCTAA AAAGGTTTAA AGAAGAACGT AGCTTACAAG TACTTACTTT ACATGCAAGA 
TCGCAATCAT CTGGTTTGAC GTTAACAAAT GCCACTCACG TTTTCATGTG TGAACCATTA 
CTAAATAGTG GAATTGAAAT GCAAGCAATT TCACGCGTTC ATCGAATTGG GCAAACTCGT 
CCGACGTTTG TTTACTACTA CATTGTGGAA GATACTGTTG AAGGTCATAT TTTAAATCTT 
TCATTGACCA AACATGAGCA GCTTGACAAA CTAGGTTTGG ATGTACCGCT AGTTGGTAAT 
ATCAATCGCA CTACTGAAGC TAGCAGCGGC GGAGAACAAG TTGATGCCGC AGAAATTAAA 
GATTGTCTTA AAATGGCTTT AAAGCGGCTC ACCACAGAGG ATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342916.1
CDS	420..4547
Translation	MDRTKRRKIEKEASLLNERNNLLESDFANCYRAFQHQLKLDQQIWRGPEDELNRLKSTIYPVVFWVDGSEKLHAYSFTQRKISLAKNLIPLFSVDLNKDTYSALKAPLKIWSKLWEDNRRLKKIIKYTSYVTVKGSELILSFGISILDSFLAPQDAILSSGSSSSYTALLDYTFLPSEDEEYSCLDINTALFYDCARKLAKSLRFANVSRDPRLSSELLPFQMRVLEWMKRREEEKFLTSNDLPPLWYHCKSLFDDRMVYVNHVYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPISLTDTCTFDQVVGKNVKYSKATLIITPSTILDQWLSEIDLHVPSLKVFHYQGIRKSNGLKSAKIFLDCDIVVTSYSDLRFELLYTESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCWTVSGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVREFCDLFGSLVCRHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQDLLSEAAKSLHFFKDRNLDLCDEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVLVFMLEQTNSTFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVELAVKELENVIYNSKYEDHGKNLPINYFGLDHFIHLRVWYVLLHKIYFFIASAYFSLKNEKFENEFYLLAQDLRRKIMSDVIIKTSKHLEEFSEKFIPKKLVKIPRLQKSYAKGLITGHGIIEDYNRLYKELNDQKEVLIKFRDRLIHLMKLPLLDQESDPTGDEYEESLNAQSEISYCIDVYRQMLSDRVAAVSGTINTFVSHETELEKYKLIESIKKSEKSLDKQAEERDKKYLLYFEEREEARPKADQYGSLINIVSRLLDASNRSTSSFETSKNMEEYERIDAMAKEQSRICQKLEKELSIIQLTYNSRIEYYKQLQEISDSLMPPPVSNISLNNYVKDDEKKQKFLNSVIIKASVILEKEISEKQDEASQTTNVAELVNQKISEMNIPGHIHLLRELEEEKSNTQRKIAHFESRRRYLTNLYEHIVLKAESHQICIICRDIIKQGFITTCGHLYCSFCLEAWLKHSSSCPMCKTKLNKNNAYYIGESRDIYSRQEFVTGFNKRDERLEILDDEAYRQISNMELKESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRFDGKSKNTCLKRFKEERSLQVLTLHARSQSSGLTLTNATHVFMCEPLLNSGIEMQAISRVHRIGQTRPTFVYYYIVEDTVEGHILNLSLTKHEQLDKLGLDVPLVGNINRTTEASSGGEQVDAAEIKDCLKMALKRLTTEDS

Target ORF information:

RefSeq Version	NM_001356173.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative ATP-dependent DNA helicase (SPAC144.05), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356173.1

ORF Insert Sequence:

ATGGACCGAA CTAAGAGGCG AAAAATAGAG AAGGAGGCTT CCTTACTAAA TGAAAGAAAC 
AACCTTTTGG AAAGTGATTT CGCCAATTGC TACCGTGCAT TCCAGCACCA ACTCAAGCTA 
GACCAGCAGA TATGGCGTGG ACCAGAGGAC GAGTTAAATC GGCTAAAGTC TACTATTTAT 
CCTGTCGTTT TTTGGGTTGA TGGATCTGAG AAACTACATG CATATAGTTT TACTCAAAGA 
AAAATTTCAT TGGCCAAAAA CTTGATTCCT TTGTTTTCTG TCGACTTGAA TAAGGATACT 
TACTCTGCTT TAAAAGCACC TCTCAAAATT TGGAGTAAGC TTTGGGAGGA TAATAGGCGG 
CTGAAGAAAA TTATAAAATA CACATCTTAT GTTACTGTAA AAGGATCCGA GTTAATTTTG 
TCGTTTGGCA TTTCCATACT CGACTCTTTT CTTGCCCCTC AAGATGCGAT TCTTTCATCT 
GGATCATCTT CCTCATACAC TGCTTTACTA GACTATACGT TCCTTCCGTC TGAAGATGAG 
GAATATTCTT GTTTAGATAT TAACACTGCA CTATTTTACG ATTGTGCAAG AAAATTGGCA 
AAAAGCCTAC GTTTCGCCAA TGTATCACGC GATCCAAGGC TTTCTTCGGA ACTTTTACCA 
TTTCAAATGC GTGTTTTAGA ATGGATGAAA CGAAGAGAAG AAGAGAAATT CCTAACCTCA 
AATGATTTAC CACCTCTATG GTACCATTGC AAAAGTCTTT TTGATGATCG AATGGTTTAC 
GTTAATCATG TTTACGGCTA TATGACATTT TCGAAAGAAA AAACATATTT GCTTGCGTCC 
GGTGATATAC GTGGAGGCAT TTTAGCCGAT GAAATGGGCA TGGGAAAAAC TTTGGAAGTC 
TTAGGATTAG TTTTGCATCA TCAGTTGCCA ATTTCTTTGA CTGATACTTG TACTTTCGAT 
CAGGTCGTCG GAAAAAATGT AAAGTATTCC AAGGCCACCC TAATAATTAC GCCTTCTACA 
ATTCTCGATC AATGGTTATC TGAGATAGAT TTGCATGTGC CATCATTGAA AGTATTCCAT 
TACCAAGGAA TCCGAAAATC CAATGGTTTG AAATCAGCTA AAATTTTTTT GGATTGTGAT 
ATAGTGGTAA CATCTTATTC TGATTTACGG TTTGAGCTAT TGTACACTGA ATCTCATTCC 
AGGACCTTAA GACATGAAAA GCGACATGTT TCTCCAAAGT CTCCATTGAT TGATGTATGT 
TGGTGGAGAA TTTGTGTTGA TGAAGCTCAG ATGGTTGAAA CTTCACAGAG TAATGTCGCA 
CAAATGATTT ATCGCATACC TCGTGTCAAT TGTTGGACTG TAAGTGGAAC TCCCGTACGT 
AGTGAAGTTG ATGACCTTTT TGGCCTTTTA TTTCTTCTTC GCTACTCCCC TATGTATTTA 
TACAAAAAAC AAGCTTGGAT GCAAATTATA GAGAAGAAAC GTGTTCGTGA ATTTTGCGAT 
CTGTTTGGCT CCCTTGTATG CAGACATTCT AAACAGGACG TTGAAGAAGA ATTAAAATTA 
CCTCCTCAAC ATCGAATCTG CATGACTACA AGACTTAGTG TAGTAGAAGA AACGAACTAC 
CAGGATTTAT TATCAGAGGC GGCCAAGTCT TTGCACTTTT TCAAAGACAG AAATTTGGAT 
TTGTGTGACG AAGAATCTAT GAGGCGTTGG TTGGTTCGTT TACGACAGGC ATGTTGCCAT 
CCGCAGGTTG GATTTGGTAA CAAATCCGCA TTTGGAGGTG GACCCATGAA AAGCATTAAT 
GATGTATTAG TTTTTATGTT GGAACAAACA AACTCTACAT TCTCCTCGTT AAACAGGAAG 
CTTTATTCCG ATAAGATTAT TGTTGGTCAA ATATATGATC ATATTAAAGA CTATAATAAA 
GCATTGGCTA TTTGGTCTGA AGTCAGAATA CCTGTCGAGC TCGCTGTCAA AGAATTAGAA 
AATGTTATTT ACAACTCTAA ATATGAGGAC CATGGTAAAA ATTTACCGAT TAATTATTTT 
GGTTTGGATC ATTTCATTCA TCTGCGTGTT TGGTATGTTC TATTGCATAA AATATACTTT 
TTTATAGCAA GCGCTTATTT TTCTTTAAAA AACGAAAAGT TTGAAAATGA ATTTTATTTG 
CTCGCGCAGG ATTTAAGAAG GAAGATTATG AGTGATGTGA TTATCAAAAC CAGCAAGCAC 
CTTGAAGAGT TTTCAGAAAA ATTTATTCCA AAGAAACTTG TCAAAATACC ACGATTACAG 
AAATCTTACG CAAAAGGTCT CATAACCGGA CATGGTATAA TTGAAGACTA TAATAGACTT 
TATAAAGAGT TGAATGATCA AAAGGAGGTG CTTATAAAAT TTAGAGATCG GCTTATCCAT 
TTGATGAAAC TTCCACTTCT GGATCAAGAA TCGGATCCGA CCGGCGATGA ATACGAAGAA 
TCATTGAATG CGCAATCAGA GATATCCTAT TGTATTGATG TTTACCGTCA AATGCTTTCA 
GATCGAGTAG CGGCAGTCAG CGGTACTATT AATACCTTTG TGTCTCATGA AACAGAACTT 
GAGAAATACA AACTTATAGA GAGTATAAAA AAATCGGAGA AATCGTTAGA CAAGCAGGCT 
GAAGAACGTG ATAAAAAATA TTTGCTTTAC TTTGAGGAAA GGGAAGAAGC AAGGCCAAAA 
GCAGATCAAT ACGGATCATT AATAAATATA GTTTCAAGAC TGTTAGATGC ATCAAATAGA 
TCCACCTCAA GTTTTGAAAC TTCTAAAAAC ATGGAAGAAT ATGAACGGAT TGATGCAATG 
GCAAAGGAGC AATCTAGAAT TTGCCAAAAA TTGGAAAAAG AATTGAGTAT AATTCAGTTG 
ACTTATAATT CAAGGATTGA GTACTATAAA CAGCTGCAGG AAATTTCTGA CTCTTTGATG 
CCTCCTCCCG TTTCAAATAT CAGTTTAAAC AATTACGTTA AAGACGACGA AAAAAAACAA 
AAATTTTTGA ACTCTGTTAT AATTAAAGCT TCGGTGATTT TGGAAAAAGA AATTTCAGAA 
AAGCAGGACG AAGCAAGTCA AACAACAAAC GTAGCAGAAC TCGTCAATCA AAAAATATCC 
GAAATGAATA TACCTGGACA TATCCATCTG CTGCGAGAGC TAGAAGAGGA AAAAAGCAAT 
ACCCAACGTA AAATTGCTCA CTTCGAATCA AGGAGAAGGT ATTTAACAAA CCTATATGAA 
CATATTGTCC TTAAAGCTGA ATCTCATCAG ATTTGTATAA TTTGTCGTGA TATAATTAAG 
CAAGGTTTTA TAACAACTTG TGGCCATTTG TACTGTAGTT TTTGTTTGGA AGCGTGGTTG 
AAGCATAGTT CGTCGTGTCC AATGTGCAAA ACCAAATTGA ACAAAAATAA TGCTTACTAT 
ATTGGCGAAA GTAGGGATAT ATACTCTCGT CAAGAGTTTG TCACTGGTTT CAACAAACGA 
GACGAAAGGC TTGAAATTCT CGACGATGAA GCATACAGGC AAATTTCTAA TATGGAGTTA 
AAGGAGTCAT TTGGATCAAA AATTGACACA ATATCTAAAC ATCTTTTGTA TTTGAAACAT 
AACGAACTTT ATCCTAAAGT TGTTGTATTC TCTCAATGGC TTGATGTTTT GGATGTTTTG 
CACAAGTCAT TTGAAGCGAA TGGTATCGTC TTCATACGTT TTGATGGAAA GTCAAAGAAC 
ACTTGCCTAA AAAGGTTTAA AGAAGAACGT AGCTTACAAG TACTTACTTT ACATGCAAGA 
TCGCAATCAT CTGGTTTGAC GTTAACAAAT GCCACTCACG TTTTCATGTG TGAACCATTA 
CTAAATAGTG GAATTGAAAT GCAAGCAATT TCACGCGTTC ATCGAATTGG GCAAACTCGT 
CCGACGTTTG TTTACTACTA CATTGTGGAA GATACTGTTG AAGGTCATAT TTTAAATCTT 
TCATTGACCA AACATGAGCA GCTTGACAAA CTAGGTTTGG ATGTACCGCT AGTTGGTAAT 
ATCAATCGCA CTACTGAAGC TAGCAGCGGC GGAGAACAAG TTGATGCCGC AGAAATTAAA 
GATTGTCTTA AAATGGCTTT AAAGCGGCTC ACCACAGAGG ATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAC144.05 ( NM_001020095.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594666.1 Schizosaccharomyces pombe 972h- ATP-dependent DNA helicase (predicted) (SPAC144.05), mRNA.

Price & Availability↑

CloneID	OSj04088
Clone ID Related Accession (Same CDS sequence)	NM_001020095.2
Accession Version	NM_001020095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	ATP-dependent DNA helicase (predicted)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020095.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGGACCGAA CTAAGAGGCG AAAAATAGAG AAGGAGGCTT CCTTACTAAA TGAAAGAAAC 
AACCTTTTGG AAAGTGATTT CGCCAATTGC TACCGTGCAT TCCAGCACCA ACTCAAGCTA 
GACCAGCAGA TATGGCGTGG ACCAGAGGAC GAGTTAAATC GGCTAAAGTC TACTATTTAT 
CCTGTCGTTT TTTGGGTTGA TGGATCTGAG AAACTACATG CATATAGTTT TACTCAAAGA 
AAAATTTCAT TGGCCAAAAA CTTGATTCCT TTGTTTTCTG TCGACTTGAA TAAGGATACT 
TACTCTGCTT TAAAAGCACC TCTCAAAATT TGGAGTAAGC TTTGGGAGGA TAATAGGCGG 
CTGAAGAAAA TTATAAAATA CACATCTTAT GTTACTGTAA AAGGATCCGA GTTAATTTTG 
TCGTTTGGCA TTTCCATACT CGACTCTTTT CTTGCCCCTC AAGATGCGAT TCTTTCATCT 
GGATCATCTT CCTCATACAC TGCTTTACTA GACTATACGT TCCTTCCGTC TGAAGATGAG 
GAATATTCTT GTTTAGATAT TAACACTGCA CTATTTTACG ATTGTGCAAG AAAATTGGCA 
AAAAGCCTAC GTTTCGCCAA TGTATCACGC GATCCAAGGC TTTCTTCGGA ACTTTTACCA 
TTTCAAATGC GTGTTTTAGA ATGGATGAAA CGAAGAGAAG AAGAGAAATT CCTAACCTCA 
AATGATTTAC CACCTCTATG GTACCATTGC AAAAGTCTTT TTGATGATCG AATGGTTTAC 
GTTAATCATG TTTACGGCTA TATGACATTT TCGAAAGAAA AAACATATTT GCTTGCGTCC 
GGTGATATAC GTGGAGGCAT TTTAGCCGAT GAAATGGGCA TGGGAAAAAC TTTGGAAGTC 
TTAGGATTAG TTTTGCATCA TCAGTTGCCA ATTTCTTTGA CTGATACTTG TACTTTCGAT 
CAGGTCGTCG GAAAAAATGT AAAGTATTCC AAGGCCACCC TAATAATTAC GCCTTCTACA 
ATTCTCGATC AATGGTTATC TGAGATAGAT TTGCATGTGC CATCATTGAA AGTATTCCAT 
TACCAAGGAA TCCGAAAATC CAATGGTTTG AAATCAGCTA AAATTTTTTT GGATTGTGAT 
ATAGTGGTAA CATCTTATTC TGATTTACGG TTTGAGCTAT TGTACACTGA ATCTCATTCC 
AGGACCTTAA GACATGAAAA GCGACATGTT TCTCCAAAGT CTCCATTGAT TGATGTATGT 
TGGTGGAGAA TTTGTGTTGA TGAAGCTCAG ATGGTTGAAA CTTCACAGAG TAATGTCGCA 
CAAATGATTT ATCGCATACC TCGTGTCAAT TGTTGGACTG TAAGTGGAAC TCCCGTACGT 
AGTGAAGTTG ATGACCTTTT TGGCCTTTTA TTTCTTCTTC GCTACTCCCC TATGTATTTA 
TACAAAAAAC AAGCTTGGAT GCAAATTATA GAGAAGAAAC GTGTTCGTGA ATTTTGCGAT 
CTGTTTGGCT CCCTTGTATG CAGACATTCT AAACAGGACG TTGAAGAAGA ATTAAAATTA 
CCTCCTCAAC ATCGAATCTG CATGACTACA AGACTTAGTG TAGTAGAAGA AACGAACTAC 
CAGGATTTAT TATCAGAGGC GGCCAAGTCT TTGCACTTTT TCAAAGACAG AAATTTGGAT 
TTGTGTGACG AAGAATCTAT GAGGCGTTGG TTGGTTCGTT TACGACAGGC ATGTTGCCAT 
CCGCAGGTTG GATTTGGTAA CAAATCCGCA TTTGGAGGTG GACCCATGAA AAGCATTAAT 
GATGTATTAG TTTTTATGTT GGAACAAACA AACTCTACAT TCTCCTCGTT AAACAGGAAG 
CTTTATTCCG ATAAGATTAT TGTTGGTCAA ATATATGATC ATATTAAAGA CTATAATAAA 
GCATTGGCTA TTTGGTCTGA AGTCAGAATA CCTGTCGAGC TCGCTGTCAA AGAATTAGAA 
AATGTTATTT ACAACTCTAA ATATGAGGAC CATGGTAAAA ATTTACCGAT TAATTATTTT 
GGTTTGGATC ATTTCATTCA TCTGCGTGTT TGGTATGTTC TATTGCATAA AATATACTTT 
TTTATAGCAA GCGCTTATTT TTCTTTAAAA AACGAAAAGT TTGAAAATGA ATTTTATTTG 
CTCGCGCAGG ATTTAAGAAG GAAGATTATG AGTGATGTGA TTATCAAAAC CAGCAAGCAC 
CTTGAAGAGT TTTCAGAAAA ATTTATTCCA AAGAAACTTG TCAAAATACC ACGATTACAG 
AAATCTTACG CAAAAGGTCT CATAACCGGA CATGGTATAA TTGAAGACTA TAATAGACTT 
TATAAAGAGT TGAATGATCA AAAGGAGGTG CTTATAAAAT TTAGAGATCG GCTTATCCAT 
TTGATGAAAC TTCCACTTCT GGATCAAGAA TCGGATCCGA CCGGCGATGA ATACGAAGAA 
TCATTGAATG CGCAATCAGA GATATCCTAT TGTATTGATG TTTACCGTCA AATGCTTTCA 
GATCGAGTAG CGGCAGTCAG CGGTACTATT AATACCTTTG TGTCTCATGA AACAGAACTT 
GAGAAATACA AACTTATAGA GAGTATAAAA AAATCGGAGA AATCGTTAGA CAAGCAGGCT 
GAAGAACGTG ATAAAAAATA TTTGCTTTAC TTTGAGGAAA GGGAAGAAGC AAGGCCAAAA 
GCAGATCAAT ACGGATCATT AATAAATATA GTTTCAAGAC TGTTAGATGC ATCAAATAGA 
TCCACCTCAA GTTTTGAAAC TTCTAAAAAC ATGGAAGAAT ATGAACGGAT TGATGCAATG 
GCAAAGGAGC AATCTAGAAT TTGCCAAAAA TTGGAAAAAG AATTGAGTAT AATTCAGTTG 
ACTTATAATT CAAGGATTGA GTACTATAAA CAGCTGCAGG AAATTTCTGA CTCTTTGATG 
CCTCCTCCCG TTTCAAATAT CAGTTTAAAC AATTACGTTA AAGACGACGA AAAAAAACAA 
AAATTTTTGA ACTCTGTTAT AATTAAAGCT TCGGTGATTT TGGAAAAAGA AATTTCAGAA 
AAGCAGGACG AAGCAAGTCA AACAACAAAC GTAGCAGAAC TCGTCAATCA AAAAATATCC 
GAAATGAATA TACCTGGACA TATCCATCTG CTGCGAGAGC TAGAAGAGGA AAAAAGCAAT 
ACCCAACGTA AAATTGCTCA CTTCGAATCA AGGAGAAGGT ATTTAACAAA CCTATATGAA 
CATATTGTCC TTAAAGCTGA ATCTCATCAG ATTTGTATAA TTTGTCGTGA TATAATTAAG 
CAAGGTTTTA TAACAACTTG TGGCCATTTG TACTGTAGTT TTTGTTTGGA AGCGTGGTTG 
AAGCATAGTT CGTCGTGTCC AATGTGCAAA ACCAAATTGA ACAAAAATAA TGCTTACTAT 
ATTGGCGAAA GTAGGGATAT ATACTCTCGT CAAGAGTTTG TCACTGGTTT CAACAAACGA 
GACGAAAGGC TTGAAATTCT CGACGATGAA GCATACAGGC AAATTTCTAA TATGGAGTTA 
AAGGAGTCAT TTGGATCAAA AATTGACACA ATATCTAAAC ATCTTTTGTA TTTGAAACAT 
AACGAACTTT ATCCTAAAGT TGTTGTATTC TCTCAATGGC TTGATGTTTT GGATGTTTTG 
CACAAGTCAT TTGAAGCGAA TGGTATCGTC TTCATACGTT TTGATGGAAA GTCAAAGAAC 
ACTTGCCTAA AAAGGTTTAA AGAAGAACGT AGCTTACAAG TACTTACTTT ACATGCAAGA 
TCGCAATCAT CTGGTTTGAC GTTAACAAAT GCCACTCACG TTTTCATGTG TGAACCATTA 
CTAAATAGTG GAATTGAAAT GCAAGCAATT TCACGCGTTC ATCGAATTGG GCAAACTCGT 
CCGACGTTTG TTTACTACTA CATTGTGGAA GATACTGTTG AAGGTCATAT TTTAAATCTT 
TCATTGACCA AACATGAGCA GCTTGACAAA CTAGGTTTGG ATGTACCGCT AGTTGGTAAT 
ATCAATCGCA CTACTGAAGC TAGCAGCGGC GGAGAACAAG TTGATGCCGC AGAAATTAAA 
GATTGTCTTA AAATGGCTTT AAAGCGGCTC ACCACAGAGG ATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594666.1
CDS	463..4590
Translation	MDRTKRRKIEKEASLLNERNNLLESDFANCYRAFQHQLKLDQQIWRGPEDELNRLKSTIYPVVFWVDGSEKLHAYSFTQRKISLAKNLIPLFSVDLNKDTYSALKAPLKIWSKLWEDNRRLKKIIKYTSYVTVKGSELILSFGISILDSFLAPQDAILSSGSSSSYTALLDYTFLPSEDEEYSCLDINTALFYDCARKLAKSLRFANVSRDPRLSSELLPFQMRVLEWMKRREEEKFLTSNDLPPLWYHCKSLFDDRMVYVNHVYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPISLTDTCTFDQVVGKNVKYSKATLIITPSTILDQWLSEIDLHVPSLKVFHYQGIRKSNGLKSAKIFLDCDIVVTSYSDLRFELLYTESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCWTVSGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVREFCDLFGSLVCRHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQDLLSEAAKSLHFFKDRNLDLCDEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVLVFMLEQTNSTFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVELAVKELENVIYNSKYEDHGKNLPINYFGLDHFIHLRVWYVLLHKIYFFIASAYFSLKNEKFENEFYLLAQDLRRKIMSDVIIKTSKHLEEFSEKFIPKKLVKIPRLQKSYAKGLITGHGIIEDYNRLYKELNDQKEVLIKFRDRLIHLMKLPLLDQESDPTGDEYEESLNAQSEISYCIDVYRQMLSDRVAAVSGTINTFVSHETELEKYKLIESIKKSEKSLDKQAEERDKKYLLYFEEREEARPKADQYGSLINIVSRLLDASNRSTSSFETSKNMEEYERIDAMAKEQSRICQKLEKELSIIQLTYNSRIEYYKQLQEISDSLMPPPVSNISLNNYVKDDEKKQKFLNSVIIKASVILEKEISEKQDEASQTTNVAELVNQKISEMNIPGHIHLLRELEEEKSNTQRKIAHFESRRRYLTNLYEHIVLKAESHQICIICRDIIKQGFITTCGHLYCSFCLEAWLKHSSSCPMCKTKLNKNNAYYIGESRDIYSRQEFVTGFNKRDERLEILDDEAYRQISNMELKESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRFDGKSKNTCLKRFKEERSLQVLTLHARSQSSGLTLTNATHVFMCEPLLNSGIEMQAISRVHRIGQTRPTFVYYYIVEDTVEGHILNLSLTKHEQLDKLGLDVPLVGNINRTTEASSGGEQVDAAEIKDCLKMALKRLTTEDS

Target ORF information:

RefSeq Version	NM_001020095.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- ATP-dependent DNA helicase (predicted) (SPAC144.05), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020095.2

ORF Insert Sequence:

ATGGACCGAA CTAAGAGGCG AAAAATAGAG AAGGAGGCTT CCTTACTAAA TGAAAGAAAC 
AACCTTTTGG AAAGTGATTT CGCCAATTGC TACCGTGCAT TCCAGCACCA ACTCAAGCTA 
GACCAGCAGA TATGGCGTGG ACCAGAGGAC GAGTTAAATC GGCTAAAGTC TACTATTTAT 
CCTGTCGTTT TTTGGGTTGA TGGATCTGAG AAACTACATG CATATAGTTT TACTCAAAGA 
AAAATTTCAT TGGCCAAAAA CTTGATTCCT TTGTTTTCTG TCGACTTGAA TAAGGATACT 
TACTCTGCTT TAAAAGCACC TCTCAAAATT TGGAGTAAGC TTTGGGAGGA TAATAGGCGG 
CTGAAGAAAA TTATAAAATA CACATCTTAT GTTACTGTAA AAGGATCCGA GTTAATTTTG 
TCGTTTGGCA TTTCCATACT CGACTCTTTT CTTGCCCCTC AAGATGCGAT TCTTTCATCT 
GGATCATCTT CCTCATACAC TGCTTTACTA GACTATACGT TCCTTCCGTC TGAAGATGAG 
GAATATTCTT GTTTAGATAT TAACACTGCA CTATTTTACG ATTGTGCAAG AAAATTGGCA 
AAAAGCCTAC GTTTCGCCAA TGTATCACGC GATCCAAGGC TTTCTTCGGA ACTTTTACCA 
TTTCAAATGC GTGTTTTAGA ATGGATGAAA CGAAGAGAAG AAGAGAAATT CCTAACCTCA 
AATGATTTAC CACCTCTATG GTACCATTGC AAAAGTCTTT TTGATGATCG AATGGTTTAC 
GTTAATCATG TTTACGGCTA TATGACATTT TCGAAAGAAA AAACATATTT GCTTGCGTCC 
GGTGATATAC GTGGAGGCAT TTTAGCCGAT GAAATGGGCA TGGGAAAAAC TTTGGAAGTC 
TTAGGATTAG TTTTGCATCA TCAGTTGCCA ATTTCTTTGA CTGATACTTG TACTTTCGAT 
CAGGTCGTCG GAAAAAATGT AAAGTATTCC AAGGCCACCC TAATAATTAC GCCTTCTACA 
ATTCTCGATC AATGGTTATC TGAGATAGAT TTGCATGTGC CATCATTGAA AGTATTCCAT 
TACCAAGGAA TCCGAAAATC CAATGGTTTG AAATCAGCTA AAATTTTTTT GGATTGTGAT 
ATAGTGGTAA CATCTTATTC TGATTTACGG TTTGAGCTAT TGTACACTGA ATCTCATTCC 
AGGACCTTAA GACATGAAAA GCGACATGTT TCTCCAAAGT CTCCATTGAT TGATGTATGT 
TGGTGGAGAA TTTGTGTTGA TGAAGCTCAG ATGGTTGAAA CTTCACAGAG TAATGTCGCA 
CAAATGATTT ATCGCATACC TCGTGTCAAT TGTTGGACTG TAAGTGGAAC TCCCGTACGT 
AGTGAAGTTG ATGACCTTTT TGGCCTTTTA TTTCTTCTTC GCTACTCCCC TATGTATTTA 
TACAAAAAAC AAGCTTGGAT GCAAATTATA GAGAAGAAAC GTGTTCGTGA ATTTTGCGAT 
CTGTTTGGCT CCCTTGTATG CAGACATTCT AAACAGGACG TTGAAGAAGA ATTAAAATTA 
CCTCCTCAAC ATCGAATCTG CATGACTACA AGACTTAGTG TAGTAGAAGA AACGAACTAC 
CAGGATTTAT TATCAGAGGC GGCCAAGTCT TTGCACTTTT TCAAAGACAG AAATTTGGAT 
TTGTGTGACG AAGAATCTAT GAGGCGTTGG TTGGTTCGTT TACGACAGGC ATGTTGCCAT 
CCGCAGGTTG GATTTGGTAA CAAATCCGCA TTTGGAGGTG GACCCATGAA AAGCATTAAT 
GATGTATTAG TTTTTATGTT GGAACAAACA AACTCTACAT TCTCCTCGTT AAACAGGAAG 
CTTTATTCCG ATAAGATTAT TGTTGGTCAA ATATATGATC ATATTAAAGA CTATAATAAA 
GCATTGGCTA TTTGGTCTGA AGTCAGAATA CCTGTCGAGC TCGCTGTCAA AGAATTAGAA 
AATGTTATTT ACAACTCTAA ATATGAGGAC CATGGTAAAA ATTTACCGAT TAATTATTTT 
GGTTTGGATC ATTTCATTCA TCTGCGTGTT TGGTATGTTC TATTGCATAA AATATACTTT 
TTTATAGCAA GCGCTTATTT TTCTTTAAAA AACGAAAAGT TTGAAAATGA ATTTTATTTG 
CTCGCGCAGG ATTTAAGAAG GAAGATTATG AGTGATGTGA TTATCAAAAC CAGCAAGCAC 
CTTGAAGAGT TTTCAGAAAA ATTTATTCCA AAGAAACTTG TCAAAATACC ACGATTACAG 
AAATCTTACG CAAAAGGTCT CATAACCGGA CATGGTATAA TTGAAGACTA TAATAGACTT 
TATAAAGAGT TGAATGATCA AAAGGAGGTG CTTATAAAAT TTAGAGATCG GCTTATCCAT 
TTGATGAAAC TTCCACTTCT GGATCAAGAA TCGGATCCGA CCGGCGATGA ATACGAAGAA 
TCATTGAATG CGCAATCAGA GATATCCTAT TGTATTGATG TTTACCGTCA AATGCTTTCA 
GATCGAGTAG CGGCAGTCAG CGGTACTATT AATACCTTTG TGTCTCATGA AACAGAACTT 
GAGAAATACA AACTTATAGA GAGTATAAAA AAATCGGAGA AATCGTTAGA CAAGCAGGCT 
GAAGAACGTG ATAAAAAATA TTTGCTTTAC TTTGAGGAAA GGGAAGAAGC AAGGCCAAAA 
GCAGATCAAT ACGGATCATT AATAAATATA GTTTCAAGAC TGTTAGATGC ATCAAATAGA 
TCCACCTCAA GTTTTGAAAC TTCTAAAAAC ATGGAAGAAT ATGAACGGAT TGATGCAATG 
GCAAAGGAGC AATCTAGAAT TTGCCAAAAA TTGGAAAAAG AATTGAGTAT AATTCAGTTG 
ACTTATAATT CAAGGATTGA GTACTATAAA CAGCTGCAGG AAATTTCTGA CTCTTTGATG 
CCTCCTCCCG TTTCAAATAT CAGTTTAAAC AATTACGTTA AAGACGACGA AAAAAAACAA 
AAATTTTTGA ACTCTGTTAT AATTAAAGCT TCGGTGATTT TGGAAAAAGA AATTTCAGAA 
AAGCAGGACG AAGCAAGTCA AACAACAAAC GTAGCAGAAC TCGTCAATCA AAAAATATCC 
GAAATGAATA TACCTGGACA TATCCATCTG CTGCGAGAGC TAGAAGAGGA AAAAAGCAAT 
ACCCAACGTA AAATTGCTCA CTTCGAATCA AGGAGAAGGT ATTTAACAAA CCTATATGAA 
CATATTGTCC TTAAAGCTGA ATCTCATCAG ATTTGTATAA TTTGTCGTGA TATAATTAAG 
CAAGGTTTTA TAACAACTTG TGGCCATTTG TACTGTAGTT TTTGTTTGGA AGCGTGGTTG 
AAGCATAGTT CGTCGTGTCC AATGTGCAAA ACCAAATTGA ACAAAAATAA TGCTTACTAT 
ATTGGCGAAA GTAGGGATAT ATACTCTCGT CAAGAGTTTG TCACTGGTTT CAACAAACGA 
GACGAAAGGC TTGAAATTCT CGACGATGAA GCATACAGGC AAATTTCTAA TATGGAGTTA 
AAGGAGTCAT TTGGATCAAA AATTGACACA ATATCTAAAC ATCTTTTGTA TTTGAAACAT 
AACGAACTTT ATCCTAAAGT TGTTGTATTC TCTCAATGGC TTGATGTTTT GGATGTTTTG 
CACAAGTCAT TTGAAGCGAA TGGTATCGTC TTCATACGTT TTGATGGAAA GTCAAAGAAC 
ACTTGCCTAA AAAGGTTTAA AGAAGAACGT AGCTTACAAG TACTTACTTT ACATGCAAGA 
TCGCAATCAT CTGGTTTGAC GTTAACAAAT GCCACTCACG TTTTCATGTG TGAACCATTA 
CTAAATAGTG GAATTGAAAT GCAAGCAATT TCACGCGTTC ATCGAATTGG GCAAACTCGT 
CCGACGTTTG TTTACTACTA CATTGTGGAA GATACTGTTG AAGGTCATAT TTTAAATCTT 
TCATTGACCA AACATGAGCA GCTTGACAAA CTAGGTTTGG ATGTACCGCT AGTTGGTAAT 
ATCAATCGCA CTACTGAAGC TAGCAGCGGC GGAGAACAAG TTGATGCCGC AGAAATTAAA 
GATTGTCTTA AAATGGCTTT AAAGCGGCTC ACCACAGAGG ATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC144.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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