pot1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	pot1
Entrez Gene ID	2542703
Full Name	telomere end-binding protein Pot1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019882.2	NP_594453.1	telomere end-binding protein Pot1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The conservation between fission yeast Tpz1-Pot1 and human TPP1-POT1 interactions resulted in mapping a human melanoma-associated POT1 mutation (A532P) to the TPP1-POT1 interface.
Title: Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis.

Long G2 phase of pot1-rqh1 double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to thiabendazole.
Title: Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in Schizosaccharomyces pombe.

The Pot1 DNA-binding domain is capable of binding DNA in at least two structurally distinct binding modes.
Title: The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3' end availability.

Pot1 promiscuity is implemented through binding modes that thermodynamically compensate for base-substitutions through alternate stacking interactions and hydrogen-bonding networks.
Title: Nonspecific recognition is achieved in Pot1pC through the use of multiple binding modes.

Results indicate an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats.
Title: DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.

The following pot1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pot1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102033	NM_001019882.2 Latest version!	Schizosaccharomyces pombe telomere end-binding protein Pot1 (pot1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pot1 ( NM_001019882.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594453.1 Schizosaccharomyces pombe telomere end-binding protein Pot1 (pot1), mRNA.

Price & Availability↑

CloneID	OSc102033
Clone ID Related Accession (Same CDS sequence)	NM_001019882.2
Accession Version	NM_001019882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	telomere end-binding protein Pot1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019882.1.

ATGGGAGAGG ACGTTATTGA CAGTCTTCAG TTGAATGAGT TATTAAATGC TGGAGAATAT 
AAGATTGGAG AACTTACATT TCAGTCCATT AGAAGCTCTC AAGAATTACA AAAGAAGAAT 
ACTATTGTCA ATTTGTTTGG AATAGTAAAA GATTTTACCC CTAGTCGCCA AAGTCTACAT 
GGAACTAAGG ATTGGGTAAC CACCGTATAT TTGTGGGATC CAACATGTGA TACATCAAGC 
ATCGGACTAC AGATACACTT GTTCAGCAAA CAGGGAAATG ATTTGCCTGT AATCAAGCAG 
GTGGGGCAAC CGCTTTTGCT TCATCAAATC ACATTAAGAA GTTATAGAGA CAGGACTCAA 
GGTTTGTCTA AGGATCAATT TCGATATGCA CTTTGGCCAG ACTTTTCTTC TAATTCCAAA 
GATACTCTCT GTCCTCAACC AATGCCTCGT TTAATGAAAA CGGGAGACAA GGAAGAGCAA 
TTCGCCTTGT TGTTAAATAA AATTTGGGAT GAGCAAACTA ATAAACATAA AAATGGCGAA 
TTATTGAGTA CCTCTTCTGC TCGTCAAAAT CAAACTGGAT TGAGTTACCC TTCTGTCTCT 
TTTTCTCTGC TATCACAAAT AACTCCACAT CAACGTTGTA GCTTTTACGC TCAGGTAATT 
AAAACTTGGT ACAGTGATAA AAACTTTACT CTTTATGTCA CTGATTATAC GGAAAATGAG 
CTTTTTTTTC CAATGTCTCC GTATACTAGC TCCTCGAGAT GGAGGGGCCC TTTTGGTCGG 
TTTTCTATAA GGTGCATTTT ATGGGATGAG CACGACTTTT ACTGCCGCAA CTACATTAAA 
GAAGGTGACT ATGTGGTTAT GAAAAATGTG CGAACCAAAA TTGATCACCT TGGTTATCTG 
GAATGTATAC TTCATGGGGA TTCAGCAAAA CGTTATAATA TGAGTATAGA AAAAGTCGAT 
TCGGAAGAAC CCGAACTAAA CGAAATTAAG TCACGTAAAA GGCTTTATGT TCAGAATTGC 
CAAAATGGTA TAGAAGCAGT AATCGAGAAA CTCAGTCAAA GCCAACAATC GGAAAATCCT 
TTTATCGCCC ATGAATTAAA GCAAACTTCT GTTAATGAAA TTACGGCCCA TGTCATAAAT 
GAACCTGCTA GTTTAAAATT GACTACTATT TCTACCATAC TTCATGCACC TTTGCAGAAT 
CTTCTCAAAC CGAGGAAACA TAGGCTACGC GTTCAGGTGG TAGATTTTTG GCCAAAGAGT 
TTGACGCAGT TTGCTGTGCT ATCTCAACCA CCATCTTCGT ATGTTTGGAT GTTTGCCTTG 
CTCGTAAGGG ATGTATCGAA TGTGACTTTA CCGGTCATAT TTTTTGATTC TGACGCTGCG 
GAACTTATTA ACAGCTCAAA AATCCAACCT TGCAATTTAG CTGATCACCC GCAGATGACT 
CTTCAGCTTA AAGAAAGATT ATTTCTGATT TGGGGGAACT TGGAAGAACG CATTCAGCAT 
CACATATCGA AGGGTGAATC GCCAACTCTG GCTGCTGAAG ATGTTGAAAC ACCATGGTTT 
GATATATATG TCAAAGAATA CATTCCTGTA ATTGGGAACA CCAAAGACCA TCAATCTTTG 
ACTTTTCTTC AGAAGCGCTG GCGAGGATTT GGCACGAAAA TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594453.1
CDS	385..2052
Translation	MGEDVIDSLQLNELLNAGEYKIGELTFQSIRSSQELQKKNTIVNLFGIVKDFTPSRQSLHGTKDWVTTVYLWDPTCDTSSIGLQIHLFSKQGNDLPVIKQVGQPLLLHQITLRSYRDRTQGLSKDQFRYALWPDFSSNSKDTLCPQPMPRLMKTGDKEEQFALLLNKIWDEQTNKHKNGELLSTSSARQNQTGLSYPSVSFSLLSQITPHQRCSFYAQVIKTWYSDKNFTLYVTDYTENELFFPMSPYTSSSRWRGPFGRFSIRCILWDEHDFYCRNYIKEGDYVVMKNVRTKIDHLGYLECILHGDSAKRYNMSIEKVDSEEPELNEIKSRKRLYVQNCQNGIEAVIEKLSQSQQSENPFIAHELKQTSVNEITAHVINEPASLKLTTISTILHAPLQNLLKPRKHRLRVQVVDFWPKSLTQFAVLSQPPSSYVWMFALLVRDVSNVTLPVIFFDSDAAELINSSKIQPCNLADHPQMTLQLKERLFLIWGNLEERIQHHISKGESPTLAAEDVETPWFDIYVKEYIPVIGNTKDHQSLTFLQKRWRGFGTKIV

Target ORF information:

RefSeq Version	NM_001019882.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe telomere end-binding protein Pot1 (pot1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019882.2

ORF Insert Sequence:

ATGGGAGAGG ACGTTATTGA CAGTCTTCAG TTGAATGAGT TATTAAATGC TGGAGAATAT 
AAGATTGGAG AACTTACATT TCAGTCCATT AGAAGCTCTC AAGAATTACA AAAGAAGAAT 
ACTATTGTCA ATTTGTTTGG AATAGTAAAA GATTTTACCC CTAGTCGCCA AAGTCTACAT 
GGAACTAAGG ATTGGGTAAC CACCGTATAT TTGTGGGATC CAACATGTGA TACATCAAGC 
ATCGGACTAC AGATACACTT GTTCAGCAAA CAGGGAAATG ATTTGCCTGT AATCAAGCAG 
GTGGGGCAAC CGCTTTTGCT TCATCAAATC ACATTAAGAA GTTATAGAGA CAGGACTCAA 
GGTTTGTCTA AGGATCAATT TCGATATGCA CTTTGGCCAG ACTTTTCTTC TAATTCCAAA 
GATACTCTCT GTCCTCAACC AATGCCTCGT TTAATGAAAA CGGGAGACAA GGAAGAGCAA 
TTCGCCTTGT TGTTAAATAA AATTTGGGAT GAGCAAACTA ATAAACATAA AAATGGCGAA 
TTATTGAGTA CCTCTTCTGC TCGTCAAAAT CAAACTGGAT TGAGTTACCC TTCTGTCTCT 
TTTTCTCTGC TATCACAAAT AACTCCACAT CAACGTTGTA GCTTTTACGC TCAGGTAATT 
AAAACTTGGT ACAGTGATAA AAACTTTACT CTTTATGTCA CTGATTATAC GGAAAATGAG 
CTTTTTTTTC CAATGTCTCC GTATACTAGC TCCTCGAGAT GGAGGGGCCC TTTTGGTCGG 
TTTTCTATAA GGTGCATTTT ATGGGATGAG CACGACTTTT ACTGCCGCAA CTACATTAAA 
GAAGGTGACT ATGTGGTTAT GAAAAATGTG CGAACCAAAA TTGATCACCT TGGTTATCTG 
GAATGTATAC TTCATGGGGA TTCAGCAAAA CGTTATAATA TGAGTATAGA AAAAGTCGAT 
TCGGAAGAAC CCGAACTAAA CGAAATTAAG TCACGTAAAA GGCTTTATGT TCAGAATTGC 
CAAAATGGTA TAGAAGCAGT AATCGAGAAA CTCAGTCAAA GCCAACAATC GGAAAATCCT 
TTTATCGCCC ATGAATTAAA GCAAACTTCT GTTAATGAAA TTACGGCCCA TGTCATAAAT 
GAACCTGCTA GTTTAAAATT GACTACTATT TCTACCATAC TTCATGCACC TTTGCAGAAT 
CTTCTCAAAC CGAGGAAACA TAGGCTACGC GTTCAGGTGG TAGATTTTTG GCCAAAGAGT 
TTGACGCAGT TTGCTGTGCT ATCTCAACCA CCATCTTCGT ATGTTTGGAT GTTTGCCTTG 
CTCGTAAGGG ATGTATCGAA TGTGACTTTA CCGGTCATAT TTTTTGATTC TGACGCTGCG 
GAACTTATTA ACAGCTCAAA AATCCAACCT TGCAATTTAG CTGATCACCC GCAGATGACT 
CTTCAGCTTA AAGAAAGATT ATTTCTGATT TGGGGGAACT TGGAAGAACG CATTCAGCAT 
CACATATCGA AGGGTGAATC GCCAACTCTG GCTGCTGAAG ATGTTGAAAC ACCATGGTTT 
GATATATATG TCAAAGAATA CATTCCTGTA ATTGGGAACA CCAAAGACCA TCAATCTTTG 
ACTTTTCTTC AGAAGCGCTG GCGAGGATTT GGCACGAAAA TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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pot1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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