tpz1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tpz1
Entrez Gene ID	2542414
Full Name	Tppl homolog Tpz1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019338.3	NP_593908.2	telomere-protecting protein
NM_001356076.1	NP_001342857.1	telomere-protecting protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together, these data provide a first glimpse into the architectural design of the Ccq1-Tpz1-Poz1 complex and reveals unique interactions that are important in maintaining the Schizosaccharomyces pombe telomere in a non-extendible state.
Title: Spatial Organization and Molecular Interactions of the Schizosaccharomyces pombe Ccq1-Tpz1-Poz1 Shelterin Complex.

The conservation between fission yeast Tpz1-Pot1 and human TPP1-POT1 interactions resulted in mapping a human melanoma-associated POT1 mutation (A532P) to the TPP1-POT1 interface.
Title: Dissecting Fission Yeast Shelterin Interactions via MICro-MS Links Disruption of Shelterin Bridge to Tumorigenesis.

Tpz1-Ccq1 interaction is required for Ccq1 Thr93 phosphorylation and telomerase recruitment.
Title: Tpz1-Ccq1 and Tpz1-Poz1 interactions within fission yeast shelterin modulate Ccq1 Thr93 phosphorylation and telomerase recruitment.

SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1.
Title: Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.

fission yeast shelterin (shelterin subunits Poz1, Rap1 and Taz1 )maintains telomere length homeostasis by coordinating the differential arrival of leading (Polepsilon) and lagging (Polalpha) strand DNA polymerases at telomeres to modulate Rad3(ATR) association
Title: Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.

The following tpz1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tpz1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj03624	NM_001019338.3 Latest version!	Schizosaccharomyces pombe 972h- telomere-protecting protein (tpz1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OSc101922	NM_001356076.1 Latest version!	Schizosaccharomyces pombe telomere-protecting protein (tpz1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tpz1 ( NM_001019338.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593908.2 Schizosaccharomyces pombe 972h- telomere-protecting protein (tpz1), mRNA.

Price & Availability↑

CloneID	OSj03624
Clone ID Related Accession (Same CDS sequence)	NM_001019338.3
Accession Version	NM_001019338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	telomere-protecting protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019338.2. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTCAAATT GCTTAAAACA TCCTTGGCTT GAGAATGGAC TATTGAATTT GATCAAAAAC 
GCAGATGTCC TTCCGATACG TGTATTTAAA TGTCAGCCTT TACAAATATT TGAGTATATA 
CGATACGAAC ATCCAATTCG ATGCAAGCTA TCCGATACAG AGTTTTACAT TGAAGCAGAG 
TTTTCGTCTC AGAGCATTTC GGACTTAAAT AATTTTACTG AAAAAAGGAT AACATCTTTA 
AGAGGAGGTA TCGTGACATT GGGCAATTTT TTAATTCACT TAATTCCTAG TCAATCAGGC 
ATTATTCCTT GGATACAAGT AGAGTCATTT AATTTTCAGG GTTGTGAGGG TGCTGTTTTT 
GGAAACCCTA AAGCTATTAC AACTTCTGCA TTGTTTAATG CATTGCTTCA ATCACCTTAT 
CTTGCTGCGC TTGCCAATGA ATTTAACCGA TCAATTAAAG AAGGATCGTC TTACCAGGAA 
GCCTCCCTTA GTCAACAGGA AAAACCTAAT GACAATACTA GTAATTCTAG AGATATCAAA 
AATAACATCC AATTTCATTG GAAAAATATG ACTTCCTTAA GTATAGAAGA ATGCATCATT 
CCAAAAGGAC AGCAACTGAT CTTAGAAAAG GAGTCTGAAG AAAATACTAC TCATGGAATT 
TACCTAGAGG AAAGAAAAAT GGCTCAAGGA CTTCATAATT CTGTTAGTGA AACCCCAGAA 
GTTAAGCAAG AGGATAACGA TGAAGATTTA GACGCGTATT CTTGGTCTTC ATCAACTGAC 
TCTGCTGGAG AAATTCCTTC TCTTCCTACA AATAGAAAAA TCCTTGAAAA AATTGCGGAG 
AAGCCTCCTC CTTTTGAAAG TCCTCTTGAA GACGACGAAA CACCTGATCA AACAAATGAG 
CACGAAGCCA ATCAAGTCAA TGTTTCACAA CTTCCACTCA ATCCACGTGG AAGCGGTATT 
TCGGGCAGAC CAGTTGAGTC AACTGAACAA TTAAATTCTT CTTTGACAAT TGAGCGCAGC 
CAGTCTATCC AATCTACTGA TTCAAAACAA AGGGTTGAAA CTCAGTCTCA CCGCCGCTCA 
AAAATTGAAA TTTTTGACGC TCAGGATGAA CTTTTTGACC GTTCAATTTG TACCACTATT 
GATGATTCTA CAGGGAAGTT GTTGAATGCT GAAGAAACTC CCATTAAAAC TGGAGACTTA 
CATAGCACTT CTGCAAGTTC AGTAATATCT TGTACACCGC CTGCTATTAA TTTTACTAGT 
GACATATGCA ATGAGCAGAT AGAGCTTGAA TACAAGAGAA AGCCTATTCC CGACTACGAT 
TTTATGAAAG GTTTAGAGAC TACGCTGCAA GAGTTGTATG TTGAGCATCA GAGCAAGAAA 
CGGCGTCTCG AACTATTTCA ATTAACAAAT AATCATCAAA AGAACTCTGA GGCCTGTGAA 
ATGTGTCGGC TTGGGCTACC TCATGGATCA TTCTTTGAGC TATTGCGAGA TTGGAAAAAA 
ATAGAGGAGT TTCGAAACAA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593908.2
CDS	518..2044
Translation	MSNCLKHPWLENGLLNLIKNADVLPIRVFKCQPLQIFEYIRYEHPIRCKLSDTEFYIEAEFSSQSISDLNNFTEKRITSLRGGIVTLGNFLIHLIPSQSGIIPWIQVESFNFQGCEGAVFGNPKAITTSALFNALLQSPYLAALANEFNRSIKEGSSYQEASLSQQEKPNDNTSNSRDIKNNIQFHWKNMTSLSIEECIIPKGQQLILEKESEENTTHGIYLEERKMAQGLHNSVSETPEVKQEDNDEDLDAYSWSSSTDSAGEIPSLPTNRKILEKIAEKPPPFESPLEDDETPDQTNEHEANQVNVSQLPLNPRGSGISGRPVESTEQLNSSLTIERSQSIQSTDSKQRVETQSHRRSKIEIFDAQDELFDRSICTTIDDSTGKLLNAEETPIKTGDLHSTSASSVISCTPPAINFTSDICNEQIELEYKRKPIPDYDFMKGLETTLQELYVEHQSKKRRLELFQLTNNHQKNSEACEMCRLGLPHGSFFELLRDWKKIEEFRNKS

Target ORF information:

RefSeq Version	NM_001019338.3
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- telomere-protecting protein (tpz1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019338.3

ORF Insert Sequence:

ATGTCAAATT GCTTAAAACA TCCTTGGCTT GAGAATGGAC TATTGAATTT GATCAAAAAC 
GCAGATGTCC TTCCGATACG TGTATTTAAA TGTCAGCCTT TACAAATATT TGAGTATATA 
CGATACGAAC ATCCAATTCG ATGCAAGCTA TCCGATACAG AGTTTTACAT TGAAGCAGAG 
TTTTCGTCTC AGAGCATTTC GGACTTAAAT AATTTTACTG AAAAAAGGAT AACATCTTTA 
AGAGGAGGTA TCGTGACATT GGGCAATTTT TTAATTCACT TAATTCCTAG TCAATCAGGC 
ATTATTCCTT GGATACAAGT AGAGTCATTT AATTTTCAGG GTTGTGAGGG TGCTGTTTTT 
GGAAACCCTA AAGCTATTAC AACTTCTGCA TTGTTTAATG CATTGCTTCA ATCACCTTAT 
CTTGCTGCGC TTGCCAATGA ATTTAACCGA TCAATTAAAG AAGGATCGTC TTACCAGGAA 
GCCTCCCTTA GTCAACAGGA AAAACCTAAT GACAATACTA GTAATTCTAG AGATATCAAA 
AATAACATCC AATTTCATTG GAAAAATATG ACTTCCTTAA GTATAGAAGA ATGCATCATT 
CCAAAAGGAC AGCAACTGAT CTTAGAAAAG GAGTCTGAAG AAAATACTAC TCATGGAATT 
TACCTAGAGG AAAGAAAAAT GGCTCAAGGA CTTCATAATT CTGTTAGTGA AACCCCAGAA 
GTTAAGCAAG AGGATAACGA TGAAGATTTA GACGCGTATT CTTGGTCTTC ATCAACTGAC 
TCTGCTGGAG AAATTCCTTC TCTTCCTACA AATAGAAAAA TCCTTGAAAA AATTGCGGAG 
AAGCCTCCTC CTTTTGAAAG TCCTCTTGAA GACGACGAAA CACCTGATCA AACAAATGAG 
CACGAAGCCA ATCAAGTCAA TGTTTCACAA CTTCCACTCA ATCCACGTGG AAGCGGTATT 
TCGGGCAGAC CAGTTGAGTC AACTGAACAA TTAAATTCTT CTTTGACAAT TGAGCGCAGC 
CAGTCTATCC AATCTACTGA TTCAAAACAA AGGGTTGAAA CTCAGTCTCA CCGCCGCTCA 
AAAATTGAAA TTTTTGACGC TCAGGATGAA CTTTTTGACC GTTCAATTTG TACCACTATT 
GATGATTCTA CAGGGAAGTT GTTGAATGCT GAAGAAACTC CCATTAAAAC TGGAGACTTA 
CATAGCACTT CTGCAAGTTC AGTAATATCT TGTACACCGC CTGCTATTAA TTTTACTAGT 
GACATATGCA ATGAGCAGAT AGAGCTTGAA TACAAGAGAA AGCCTATTCC CGACTACGAT 
TTTATGAAAG GTTTAGAGAC TACGCTGCAA GAGTTGTATG TTGAGCATCA GAGCAAGAAA 
CGGCGTCTCG AACTATTTCA ATTAACAAAT AATCATCAAA AGAACTCTGA GGCCTGTGAA 
ATGTGTCGGC TTGGGCTACC TCATGGATCA TTCTTTGAGC TATTGCGAGA TTGGAAAAAA 
ATAGAGGAGT TTCGAAACAA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpz1 ( NM_001356076.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342857.1 Schizosaccharomyces pombe telomere-protecting protein (tpz1), mRNA.

Price & Availability↑

CloneID	OSc101922
Clone ID Related Accession (Same CDS sequence)	NM_001356076.1
Accession Version	NM_001356076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	telomere-protecting protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGTCAAATT GCTTAAAACA TCCTTGGCTT GAGAATGGAC TATTGAATTT GATCAAAAAC 
GCAGATGTCC TTCCGATACG TGTATTTAAA TGTCAGCCTT TACAAATATT TGAGTATATA 
CGATACGAAC ATCCAATTCG ATGCAAGCTA TCCGATACAG AGTTTTACAT TGAAGCAGAG 
TTTTCGTCTC AGAGCATTTC GGACTTAAAT AATTTTACTG AAAAAAGGAT AACATCTTTA 
AGAGGAGGTA TCGTGACATT GGGCAATTTT TTAATTCACT TAATTCCTAG TCAATCAGGC 
ATTATTCCTT GGATACAAGT AGAGTCATTT AATTTTCAGG GTTGTGAGGG TGCTGTTTTT 
GGAAACCCTA AAGCTATTAC AACTTCTGCA TTGTTTAATG CATTGCTTCA ATCACCTTAT 
CTTGCTGCGC TTGCCAATGA ATTTAACCGA TCAATTAAAG AAGGATCGTC TTACCAGGAA 
GCCTCCCTTA GTCAACAGGA AAAACCTAAT GACAATACTA GTAATTCTAG AGATATCAAA 
AATAACATCC AATTTCATTG GAAAAATATG ACTTCCTTAA GTATAGAAGA ATGCATCATT 
CCAAAAGGAC AGCAACTGAT CTTAGAAAAG GAGTCTGAAG AAAATACTAC TCATGGAATT 
TACCTAGAGG AAAGAAAAAT GGCTCAAGGA CTTCATAATT CTGTTAGTGA AACCCCAGAA 
GTTAAGCAAG AGGATAACGA TGAAGATTTA GACGCGTATT CTTGGTCTTC ATCAACTGAC 
TCTGCTGGAG AAATTCCTTC TCTTCCTACA AATAGAAAAA TCCTTGAAAA AATTGCGGAG 
AAGCCTCCTC CTTTTGAAAG TCCTCTTGAA GACGACGAAA CACCTGATCA AACAAATGAG 
CACGAAGCCA ATCAAGTCAA TGTTTCACAA CTTCCACTCA ATCCACGTGG AAGCGGTATT 
TCGGGCAGAC CAGTTGAGTC AACTGAACAA TTAAATTCTT CTTTGACAAT TGAGCGCAGC 
CAGTCTATCC AATCTACTGA TTCAAAACAA AGGGTTGAAA CTCAGTCTCA CCGCCGCTCA 
AAAATTGAAA TTTTTGACGC TCAGGATGAA CTTTTTGACC GTTCAATTTG TACCACTATT 
GATGATTCTA CAGGGAAGTT GTTGAATGCT GAAGAAACTC CCATTAAAAC TGGAGACTTA 
CATAGCACTT CTGCAAGTTC AGTAATATCT TGTACACCGC CTGCTATTAA TTTTACTAGT 
GACATATGCA ATGAGCAGAT AGAGCTTGAA TACAAGAGAA AGCCTATTCC CGACTACGAT 
TTTATGAAAG GTTTAGAGAC TACGCTGCAA GAGTTGTATG TTGAGCATCA GAGCAAGAAA 
CGGCGTCTCG AACTATTTCA ATTAACAAAT AATCATCAAA AGAACTCTGA GGCCTGTGAA 
ATGTGTCGGC TTGGGCTACC TCATGGATCA TTCTTTGAGC TATTGCGAGA TTGGAAAAAA 
ATAGAGGAGT TTCGAAACAA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342857.1
CDS	421..1947
Translation	MSNCLKHPWLENGLLNLIKNADVLPIRVFKCQPLQIFEYIRYEHPIRCKLSDTEFYIEAEFSSQSISDLNNFTEKRITSLRGGIVTLGNFLIHLIPSQSGIIPWIQVESFNFQGCEGAVFGNPKAITTSALFNALLQSPYLAALANEFNRSIKEGSSYQEASLSQQEKPNDNTSNSRDIKNNIQFHWKNMTSLSIEECIIPKGQQLILEKESEENTTHGIYLEERKMAQGLHNSVSETPEVKQEDNDEDLDAYSWSSSTDSAGEIPSLPTNRKILEKIAEKPPPFESPLEDDETPDQTNEHEANQVNVSQLPLNPRGSGISGRPVESTEQLNSSLTIERSQSIQSTDSKQRVETQSHRRSKIEIFDAQDELFDRSICTTIDDSTGKLLNAEETPIKTGDLHSTSASSVISCTPPAINFTSDICNEQIELEYKRKPIPDYDFMKGLETTLQELYVEHQSKKRRLELFQLTNNHQKNSEACEMCRLGLPHGSFFELLRDWKKIEEFRNKS

Target ORF information:

RefSeq Version	NM_001356076.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe telomere-protecting protein (tpz1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356076.1

ORF Insert Sequence:

ATGTCAAATT GCTTAAAACA TCCTTGGCTT GAGAATGGAC TATTGAATTT GATCAAAAAC 
GCAGATGTCC TTCCGATACG TGTATTTAAA TGTCAGCCTT TACAAATATT TGAGTATATA 
CGATACGAAC ATCCAATTCG ATGCAAGCTA TCCGATACAG AGTTTTACAT TGAAGCAGAG 
TTTTCGTCTC AGAGCATTTC GGACTTAAAT AATTTTACTG AAAAAAGGAT AACATCTTTA 
AGAGGAGGTA TCGTGACATT GGGCAATTTT TTAATTCACT TAATTCCTAG TCAATCAGGC 
ATTATTCCTT GGATACAAGT AGAGTCATTT AATTTTCAGG GTTGTGAGGG TGCTGTTTTT 
GGAAACCCTA AAGCTATTAC AACTTCTGCA TTGTTTAATG CATTGCTTCA ATCACCTTAT 
CTTGCTGCGC TTGCCAATGA ATTTAACCGA TCAATTAAAG AAGGATCGTC TTACCAGGAA 
GCCTCCCTTA GTCAACAGGA AAAACCTAAT GACAATACTA GTAATTCTAG AGATATCAAA 
AATAACATCC AATTTCATTG GAAAAATATG ACTTCCTTAA GTATAGAAGA ATGCATCATT 
CCAAAAGGAC AGCAACTGAT CTTAGAAAAG GAGTCTGAAG AAAATACTAC TCATGGAATT 
TACCTAGAGG AAAGAAAAAT GGCTCAAGGA CTTCATAATT CTGTTAGTGA AACCCCAGAA 
GTTAAGCAAG AGGATAACGA TGAAGATTTA GACGCGTATT CTTGGTCTTC ATCAACTGAC 
TCTGCTGGAG AAATTCCTTC TCTTCCTACA AATAGAAAAA TCCTTGAAAA AATTGCGGAG 
AAGCCTCCTC CTTTTGAAAG TCCTCTTGAA GACGACGAAA CACCTGATCA AACAAATGAG 
CACGAAGCCA ATCAAGTCAA TGTTTCACAA CTTCCACTCA ATCCACGTGG AAGCGGTATT 
TCGGGCAGAC CAGTTGAGTC AACTGAACAA TTAAATTCTT CTTTGACAAT TGAGCGCAGC 
CAGTCTATCC AATCTACTGA TTCAAAACAA AGGGTTGAAA CTCAGTCTCA CCGCCGCTCA 
AAAATTGAAA TTTTTGACGC TCAGGATGAA CTTTTTGACC GTTCAATTTG TACCACTATT 
GATGATTCTA CAGGGAAGTT GTTGAATGCT GAAGAAACTC CCATTAAAAC TGGAGACTTA 
CATAGCACTT CTGCAAGTTC AGTAATATCT TGTACACCGC CTGCTATTAA TTTTACTAGT 
GACATATGCA ATGAGCAGAT AGAGCTTGAA TACAAGAGAA AGCCTATTCC CGACTACGAT 
TTTATGAAAG GTTTAGAGAC TACGCTGCAA GAGTTGTATG TTGAGCATCA GAGCAAGAAA 
CGGCGTCTCG AACTATTTCA ATTAACAAAT AATCATCAAA AGAACTCTGA GGCCTGTGAA 
ATGTGTCGGC TTGGGCTACC TCATGGATCA TTCTTTGAGC TATTGCGAGA TTGGAAAAAA 
ATAGAGGAGT TTCGAAACAA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tpz1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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