ORF » Species Summary » Schizosaccharomyces pombe » tif224 cDNA ORF clone

tif224 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following tif224 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tif224 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103694 NM_001019932.2
Latest version!		 Schizosaccharomyces pombe putative translation initiation factor eIF2B delta subunit tif224 (tif224), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    tif224 ( NM_001019932.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594503.1 Schizosaccharomyces pombe putative translation initiation factor eIF2B delta subunit tif224 (tif224), mRNA.
    Price & Availability↑
    CloneID OSc103694
    Clone ID Related Accession (Same CDS sequence) NM_001019932.2
    Accession Version NM_001019932.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative translation initiation factor eIF2B delta subunit tif224
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019932.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGTTCT CAGCAGAACA AGCAAAAAAG GATGGGAAGG ATCAAAGCCC TGTTAGTGAA 
    AGCTCTAGTG TGGGAGGAAC ATCTCCAGCT ACAGCTTCTT CTGTCGTTTC TCCCAATGAG 
    CCAAAACTTT CAGGGAAGGA GGCAAAAGCG TTGAAAAAGG CCAGAAAACA AGCAAGTAGA 
    AGAGCAAAAG CCGAAGCAGC TGCCGCGAAT AATCCACCCG GTGTTTCAGA AGAAAAAAAG 
    GTAGCTATCC CTAATAAAAA TAGTAACCAA CAAAAGAAGG CATCGAAACA AAACCCGCAG 
    AATAGTCCAG AAACTGATGC TAATTTGCAA GAAAAAAAGA TTTTTGAGGA AAAGCAAGTT 
    TCTATCTTTT CGCACCTCGA TTGGAGACGT CGTAGAACCA CTGAAAATAT TCCGAAAGAT 
    ATCCATCCTG CTGTTATTCG TTTGGGTTTA AAACTTGCAA ATTATAAGAT TTTTGGATCG 
    AATCAGCGCT GTATTGATTT GTTGAAAACT TTTAAAATCG TCATCCAAGA TTATCAAACA 
    CCCTACGGTA CGACGTTGAG CAGGCATCTG ACCACGCATA TAAATTCTCA AATTGCTTAT 
    TTAGTATCAA CTCGTCCGTT GAGTATCAGT ATGGGGAACG CCATTCGCTT TTTAAAACTC 
    GAAATTTCTG TTTTAGACAT TGACCTTACC GACGACGAGG GAAAAGAATT GCTTCTGGAA 
    AAAATTGACA GTTATATTCG CGATCGTATT ATCATTGCAG GACAAGTCAT CGTACAAGCA 
    GCAACCGAAA AAATACAAGA CGGAGATGTC ATTTTAACGT ACTTGCACTC TTCGACAGTA 
    AACGATGTTC TCATTCATGC TAAGAATGTT GGTAAAAAAT TCCGGGTTGT CGTGGTAGAC 
    TCTCGTCCAG AATTTGAAGG CCGGGTTTGT TTGAAGCTGT TGACCGAGCA TGGGATTGAA 
    TGTACCTATG TCATGATTTC AGCGCTTTCC TATATCATGC AAGAAGTGAC CAAAATTTTC 
    CTTGGAGGCC ATGCTATGCT TTCGAATGGT GCTCTATATT CTCGTGCCGG TACCTCTTTA 
    ATTTCCCTTT TGGGACACGA GTCTAATGTT CCAGTAATAG CGTGTTGCGA GTCTTATAAA 
    TTTACTGAGC GCATTCAATT GGATTCGTTG GTATACAATG AACTTGCACC TGGCGATCAA 
    CTTGTAAACA TGGGTGTGGA TGACTTTGAA GAAAAACCTG GTGTACTCGC AAATTGGAAA 
    AGTGTTAAGA ACTTAAAATT ACTCAGTCTA AAATACGATG TTACTCCACC TCGTCTAATT 
    ACTGTATGTG TTTGTGAAAT GGGGCTTTTA CCTTCAACCT CGGTACCGGC TATCATAAAC 
    GAGTTCAAAC AGGTTTATGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594503.1
    CDS50..1453
    Translation

    Target ORF information:

    RefSeq Version NM_001019932.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative translation initiation factor eIF2B delta subunit tif224 (tif224), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001019932.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGTTCT CAGCAGAACA AGCAAAAAAG GATGGGAAGG ATCAAAGCCC TGTTAGTGAA 
    AGCTCTAGTG TGGGAGGAAC ATCTCCAGCT ACAGCTTCTT CTGTCGTTTC TCCCAATGAG 
    CCAAAACTTT CAGGGAAGGA GGCAAAAGCG TTGAAAAAGG CCAGAAAACA AGCAAGTAGA 
    AGAGCAAAAG CCGAAGCAGC TGCCGCGAAT AATCCACCCG GTGTTTCAGA AGAAAAAAAG 
    GTAGCTATCC CTAATAAAAA TAGTAACCAA CAAAAGAAGG CATCGAAACA AAACCCGCAG 
    AATAGTCCAG AAACTGATGC TAATTTGCAA GAAAAAAAGA TTTTTGAGGA AAAGCAAGTT 
    TCTATCTTTT CGCACCTCGA TTGGAGACGT CGTAGAACCA CTGAAAATAT TCCGAAAGAT 
    ATCCATCCTG CTGTTATTCG TTTGGGTTTA AAACTTGCAA ATTATAAGAT TTTTGGATCG 
    AATCAGCGCT GTATTGATTT GTTGAAAACT TTTAAAATCG TCATCCAAGA TTATCAAACA 
    CCCTACGGTA CGACGTTGAG CAGGCATCTG ACCACGCATA TAAATTCTCA AATTGCTTAT 
    TTAGTATCAA CTCGTCCGTT GAGTATCAGT ATGGGGAACG CCATTCGCTT TTTAAAACTC 
    GAAATTTCTG TTTTAGACAT TGACCTTACC GACGACGAGG GAAAAGAATT GCTTCTGGAA 
    AAAATTGACA GTTATATTCG CGATCGTATT ATCATTGCAG GACAAGTCAT CGTACAAGCA 
    GCAACCGAAA AAATACAAGA CGGAGATGTC ATTTTAACGT ACTTGCACTC TTCGACAGTA 
    AACGATGTTC TCATTCATGC TAAGAATGTT GGTAAAAAAT TCCGGGTTGT CGTGGTAGAC 
    TCTCGTCCAG AATTTGAAGG CCGGGTTTGT TTGAAGCTGT TGACCGAGCA TGGGATTGAA 
    TGTACCTATG TCATGATTTC AGCGCTTTCC TATATCATGC AAGAAGTGAC CAAAATTTTC 
    CTTGGAGGCC ATGCTATGCT TTCGAATGGT GCTCTATATT CTCGTGCCGG TACCTCTTTA 
    ATTTCCCTTT TGGGACACGA GTCTAATGTT CCAGTAATAG CGTGTTGCGA GTCTTATAAA 
    TTTACTGAGC GCATTCAATT GGATTCGTTG GTATACAATG AACTTGCACC TGGCGATCAA 
    CTTGTAAACA TGGGTGTGGA TGACTTTGAA GAAAAACCTG GTGTACTCGC AAATTGGAAA 
    AGTGTTAAGA ACTTAAAATT ACTCAGTCTA AAATACGATG TTACTCCACC TCGTCTAATT 
    ACTGTATGTG TTTGTGAAAT GGGGCTTTTA CCTTCAACCT CGGTACCGGC TATCATAAAC 
    GAGTTCAAAC AGGTTTATGC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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