SPAC20G8.02 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC20G8.02
Entrez Gene ID	2541976
Full Name	mitochondrial DDHD family phospholipase (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018750.2	NP_593319.1	putative DDHD family phospholipase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_13817	XP_003710982.1
Neurospora crassa	NCU03987	XP_956572.1
Kluyveromyces lactis	KLLA0E09373g	XP_454373.1
Eremothecium gossypii	AGOS_AAR110C	NP_982652.1
Schizosaccharomyces pombe (fission yeast)	SPAC20G8.02	NP_593319.1
Saccharomyces cerevisiae (baker's yeast)	YOR022C	NP_014665.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC20G8.02 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC20G8.02 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102180	NM_001018750.2 Latest version!	Schizosaccharomyces pombe putative DDHD family phospholipase (SPAC20G8.02), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC20G8.02 ( NM_001018750.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593319.1 Schizosaccharomyces pombe putative DDHD family phospholipase (SPAC20G8.02), partial mRNA.

Price & Availability↑

CloneID	OSc102180
Clone ID Related Accession (Same CDS sequence)	NM_001018750.2
Accession Version	NM_001018750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative DDHD family phospholipase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018750.1. COMPLETENESS: incomplete on the 5' end.

ATGATTTTAT ACATTGTATT ACCATTTTAC GTTCGTACCA AGCCTTACTC TATTTTGCCG 
TCATATTCCT CCTTACAGTG GATTACGAAT GCTTGGTGTT CATTACCGTC CATATACATT 
TTATGTCACT GTAGCAAACA CCAAGAAGAA ATTACAATAT TGGCTTTAAA GCCATTAATT 
GCTATGAATA ATTCAGAAGA ATGGCCATTG AAACATGTGC AACCGCCCAA ATTGTCATTT 
CGTCATTTGC TATCGAGATC TGTGACAGAT GCTCCTTCAT TGCGGGTACG TTGGTTCTAT 
GCGGTAGATA GACCGCTTAG GAAATCAAGA ACGGGACCGA CGGAAATTAA AAAAGCAAAA 
AATTTTTTAC CTTTTTCAGC TGAAGACTCG GAGCACATCG AAAAAAGTTA TTTAAAGGCT 
GTAGAAAATG ACGGTCAATC TGAACCGGTC AATGTGAATG AGGATTATTT GTATTCGGTG 
AACGTTGTTT CAAGAGAACT CTCACCAATA TACTGGGATG GTCCCGTTTA CCGGATACTC 
AGGGGAACGT GGTTTTTTAG TCGTGGGGAT AAACTGTACC CTTGTGAGGA AAATCTAGCG 
ACACAAGTGG AAGAAGGTTA TTTAAACAGT TGTCCCTATC GAGAATTTTC GAATGAAAAA 
GACTCTGCTG CTGCACAATC AAAAACATGG GCCCTCCTTG GAAGGTATAC AGGTGGATTC 
GTACAATACA CTGGCAGCAG GAATGCCCGA CTGGTGTATG ATGACTTTTA TCGTAACGTT 
TCTGTGAAAA TTATGAATCG GTTTTCTCCT GCTAGCTTTC ACAGAAGTGA TAAATTGGTG 
CGAGGCTATG AACTGGATAT GTTGGAGTCG AATTCGAAGC CGAGTACACC TGTCCCTACT 
GAGGAATTGA CTTCTACCAC TCTTTTAAAC GATTCTTCAG ATCCAAGTGA CAACTTTACA 
CCTAGTAATA CCGAAAGTAC GATAGACCTT CCATCCGCGA CTGATGCCTC TCATTTAATG 
TCAAGACCCG ACCGGGAAGT TAATCATCTA ATATTGTGCT GTCATGGCAT CGGTCAAAAA 
ATGGGTGAAC GTGTGGAAAC TGTCAGTTTT GTAAAGGATA TTAGTAACTT TCGTAAAACT 
TTAAAAAAAA CCTTTAATTC TTCTCCAGAC CTGCAAGCTG TGTATCCCAA ACTGAAGGGA 
GGTGGAAACG GTGTTCAATG TCTGCCTCTG TTATGGCGTC AAGACATAAG ATTTGGCATG 
GCAAGGGACC TTGATTCGTC TTTTGCGGAC GATGATGACG ATGATGATGA ATCATTGAAC 
ATGTCTAGAG ATCTTGCATT AGACGATTTG GAAGACGATT CCATTCCAAC TTTGGATAAT 
ATAAATATCC CTACGGTTAC TGGATTGCGA AACATTATTT CGGATGTTTT GCTAGACGTC 
CTCTTGTATT GTCAGCCAAA TTATCGAGAT AAAATTTTGG CAGCAGTGGT GAAGCGACTG 
AATCGTCTGT ACAATCTGTA CAAAAAAAAT GTCCCCTCAT TTAATGGCCA TGTTTCACTT 
CTTGGACATT CCTTGGGGGC TCTAATTTTA TTTGATATTA TACGTTACCA AGGAAATATC 
AAATACTCAA AACTGCAACT TGACTTTCCT GTTGCAAACT TTTTCGCCTT AGGTAGCCCA 
TTGGGATTAT TTCAAATGCT GAATGGTAAA AAAATTGCAG GTCCTATTCC TAAAACGAAT 
TTGACTCGCA GTCTAAGTTA TTCTGAGCAA AGTTTCGATT CGGGTGTCTC GATCCTTAGC 
TGTCAAAACT TCTATAATAT ATTTCATCCC ACTGACCCCA TCAGTTATCG TGTAGAGCCA 
TTGGTGGTTA AGCAAATGGC CCGCTTAAAA CCTCAGAAAA TTTCTCACTT TCGCCCTCAT 
CAAGACCTTT CAAGTTCTGG TGTAGGGCAT AAAATTGCCG GTGGTGCTCT GAATGTTCTT 
TCTGGCCTTC GATCTGGTAT TGCTAATACC CTAATCTTAA AATCATTGAG TTATGCTTCT 
GTGTTTAACG AGGCCACCGC TGATTCTCAC GATGAATCAC AGGGAGCTGA AAACATTCGG 
GATTGGCATA TTGATGAGCG AATGTATCGA TTGAACAAAA CGGGACGTAT AGACTTCATG 
CTACAAGAAG GTGCACTAGA TACTTCTTAT AGCTATGTCT CAGCAATGAA TGCTCACTCT 
GAATATTGGA AAAATGTTGA TCTTGCTCAT TTTATTCTAA CCCAATTACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593319.1
CDS	1..2274
Translation	MILYIVLPFYVRTKPYSILPSYSSLQWITNAWCSLPSIYILCHCSKHQEEITILALKPLIAMNNSEEWPLKHVQPPKLSFRHLLSRSVTDAPSLRVRWFYAVDRPLRKSRTGPTEIKKAKNFLPFSAEDSEHIEKSYLKAVENDGQSEPVNVNEDYLYSVNVVSRELSPIYWDGPVYRILRGTWFFSRGDKLYPCEENLATQVEEGYLNSCPYREFSNEKDSAAAQSKTWALLGRYTGGFVQYTGSRNARLVYDDFYRNVSVKIMNRFSPASFHRSDKLVRGYELDMLESNSKPSTPVPTEELTSTTLLNDSSDPSDNFTPSNTESTIDLPSATDASHLMSRPDREVNHLILCCHGIGQKMGERVETVSFVKDISNFRKTLKKTFNSSPDLQAVYPKLKGGGNGVQCLPLLWRQDIRFGMARDLDSSFADDDDDDDESLNMSRDLALDDLEDDSIPTLDNINIPTVTGLRNIISDVLLDVLLYCQPNYRDKILAAVVKRLNRLYNLYKKNVPSFNGHVSLLGHSLGALILFDIIRYQGNIKYSKLQLDFPVANFFALGSPLGLFQMLNGKKIAGPIPKTNLTRSLSYSEQSFDSGVSILSCQNFYNIFHPTDPISYRVEPLVVKQMARLKPQKISHFRPHQDLSSSGVGHKIAGGALNVLSGLRSGIANTLILKSLSYASVFNEATADSHDESQGAENIRDWHIDERMYRLNKTGRIDFMLQEGALDTSYSYVSAMNAHSEYWKNVDLAHFILTQLL

Target ORF information:

RefSeq Version	NM_001018750.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative DDHD family phospholipase (SPAC20G8.02), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018750.2

ORF Insert Sequence:

ATGATTTTAT ACATTGTATT ACCATTTTAC GTTCGTACCA AGCCTTACTC TATTTTGCCG 
TCATATTCCT CCTTACAGTG GATTACGAAT GCTTGGTGTT CATTACCGTC CATATACATT 
TTATGTCACT GTAGCAAACA CCAAGAAGAA ATTACAATAT TGGCTTTAAA GCCATTAATT 
GCTATGAATA ATTCAGAAGA ATGGCCATTG AAACATGTGC AACCGCCCAA ATTGTCATTT 
CGTCATTTGC TATCGAGATC TGTGACAGAT GCTCCTTCAT TGCGGGTACG TTGGTTCTAT 
GCGGTAGATA GACCGCTTAG GAAATCAAGA ACGGGACCGA CGGAAATTAA AAAAGCAAAA 
AATTTTTTAC CTTTTTCAGC TGAAGACTCG GAGCACATCG AAAAAAGTTA TTTAAAGGCT 
GTAGAAAATG ACGGTCAATC TGAACCGGTC AATGTGAATG AGGATTATTT GTATTCGGTG 
AACGTTGTTT CAAGAGAACT CTCACCAATA TACTGGGATG GTCCCGTTTA CCGGATACTC 
AGGGGAACGT GGTTTTTTAG TCGTGGGGAT AAACTGTACC CTTGTGAGGA AAATCTAGCG 
ACACAAGTGG AAGAAGGTTA TTTAAACAGT TGTCCCTATC GAGAATTTTC GAATGAAAAA 
GACTCTGCTG CTGCACAATC AAAAACATGG GCCCTCCTTG GAAGGTATAC AGGTGGATTC 
GTACAATACA CTGGCAGCAG GAATGCCCGA CTGGTGTATG ATGACTTTTA TCGTAACGTT 
TCTGTGAAAA TTATGAATCG GTTTTCTCCT GCTAGCTTTC ACAGAAGTGA TAAATTGGTG 
CGAGGCTATG AACTGGATAT GTTGGAGTCG AATTCGAAGC CGAGTACACC TGTCCCTACT 
GAGGAATTGA CTTCTACCAC TCTTTTAAAC GATTCTTCAG ATCCAAGTGA CAACTTTACA 
CCTAGTAATA CCGAAAGTAC GATAGACCTT CCATCCGCGA CTGATGCCTC TCATTTAATG 
TCAAGACCCG ACCGGGAAGT TAATCATCTA ATATTGTGCT GTCATGGCAT CGGTCAAAAA 
ATGGGTGAAC GTGTGGAAAC TGTCAGTTTT GTAAAGGATA TTAGTAACTT TCGTAAAACT 
TTAAAAAAAA CCTTTAATTC TTCTCCAGAC CTGCAAGCTG TGTATCCCAA ACTGAAGGGA 
GGTGGAAACG GTGTTCAATG TCTGCCTCTG TTATGGCGTC AAGACATAAG ATTTGGCATG 
GCAAGGGACC TTGATTCGTC TTTTGCGGAC GATGATGACG ATGATGATGA ATCATTGAAC 
ATGTCTAGAG ATCTTGCATT AGACGATTTG GAAGACGATT CCATTCCAAC TTTGGATAAT 
ATAAATATCC CTACGGTTAC TGGATTGCGA AACATTATTT CGGATGTTTT GCTAGACGTC 
CTCTTGTATT GTCAGCCAAA TTATCGAGAT AAAATTTTGG CAGCAGTGGT GAAGCGACTG 
AATCGTCTGT ACAATCTGTA CAAAAAAAAT GTCCCCTCAT TTAATGGCCA TGTTTCACTT 
CTTGGACATT CCTTGGGGGC TCTAATTTTA TTTGATATTA TACGTTACCA AGGAAATATC 
AAATACTCAA AACTGCAACT TGACTTTCCT GTTGCAAACT TTTTCGCCTT AGGTAGCCCA 
TTGGGATTAT TTCAAATGCT GAATGGTAAA AAAATTGCAG GTCCTATTCC TAAAACGAAT 
TTGACTCGCA GTCTAAGTTA TTCTGAGCAA AGTTTCGATT CGGGTGTCTC GATCCTTAGC 
TGTCAAAACT TCTATAATAT ATTTCATCCC ACTGACCCCA TCAGTTATCG TGTAGAGCCA 
TTGGTGGTTA AGCAAATGGC CCGCTTAAAA CCTCAGAAAA TTTCTCACTT TCGCCCTCAT 
CAAGACCTTT CAAGTTCTGG TGTAGGGCAT AAAATTGCCG GTGGTGCTCT GAATGTTCTT 
TCTGGCCTTC GATCTGGTAT TGCTAATACC CTAATCTTAA AATCATTGAG TTATGCTTCT 
GTGTTTAACG AGGCCACCGC TGATTCTCAC GATGAATCAC AGGGAGCTGA AAACATTCGG 
GATTGGCATA TTGATGAGCG AATGTATCGA TTGAACAAAA CGGGACGTAT AGACTTCATG 
CTACAAGAAG GTGCACTAGA TACTTCTTAT AGCTATGTCT CAGCAATGAA TGCTCACTCT 
GAATATTGGA AAAATGTTGA TCTTGCTCAT TTTATTCTAA CCCAATTACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC20G8.02 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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