thi4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following thi4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thi4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102491 NM_001018828.2
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Schizosaccharomyces pombe thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase (thi4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc102491
    Clone ID Related Accession (Same CDS sequence) NM_001018828.2
    Accession Version NM_001018828.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018828.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAACGTC AAATTGACTA TTCTTTATAC CTTGTAACTA GCTCTAGTTT AATTGCCCCA 
    GGTTCTACTA TAGAACGTCA GGTGGAAGAG GGAATTTTGG GAGGTGTCAC GCTTGTTCAG
    CACAGAGAAA AAGACATCTC TACTAAATGC TTTGTAGAAC GTGCTAAACG ACTTTCTGAA
    ATTTGCAAAA AGTACGACGT GCCTTTTCTA ATTAATGATA GGATTGACGT GGCATTAGCT
    GTTGGCGCTG ACGGTGTTCA TATTGGTCAA GACGACATGG ATTGTGCATT AGCCAGAAAA
    ATTCTTGGCG ATGATGCTAT TATTGGAGTG TCCACTAATA ATATCGAGGA AATTGAAAAA
    GCAGCTGCTG ATGGGGCCGA TTATGTGGGT ATCGGTTCTA TTTACGAGAC AAACACTAAA
    GATGTCAAAG ACAGGTTAAT AGGAATCACA GGTCTGCGAA AAATTCTTGA ACATGTTTCT
    AAAATGCATT GTCAACTTGG TACAGTGGCA ATTGCTGGTT TGAACTCATC GAATATCCAA
    AGGGTCATTT ATTTGAGCGA AGCCAATGGA AAAAGAATTG ACGGAATTGC TCTTGTTTCT
    GCTATCATGT GCTCCATTAC TCCTAGAGAA ACCGCTAAAG AACTCCGTAA CCTTATAGCC
    ACTCCTCCAT GCTTTGCTCA AGCACGTTCG TCTCTTACAA CTCCCAAGGA TTTACTGAAC
    CAAATTCCTG CTGCTTTACA GAAGTTGAAG GATTTCACTC CTCTAATTCA TCATCTGACT
    AATGCTGTTG CAAAAAACTT TTCTGCTAAC GTTACTCTTG CAGCATATGG TTCTCCAACT
    ATGGGTGAAA GCTACGATGA AGTTGCTGAT TTTGCAAAAG CCCCAGGTGC TCTTGTATTG
    AATATTGGAA TTCTTGAAAA TACAAAGACG TATATTCATG CTGCTCAAGT CAACAATGAT
    TTAGCCAGGC CCGTTATTTT AGATCCAGTT GCCGTAGGTG CTACAACCGC TCGTTCAAAA
    GTTATTAATA CTTTGTTAAA TTATGCTTAC TATGATATTA TCAAAGGGAA TGAAGGAGAA
    ATTATGAACT TAGCAGGTGA GCAAGGTTTG ATGAGAGGAG TAGATAGTAT TTCCCAGCAT
    ACATTAGCTG CTCGCATTAC AGCTGTTCAC CGCCTTGCTG TTGAAAGACG CTGTGTAGTA
    GCAATGTCTG GTGCAGTTGA TGTTATCAGC GATGGAAATT CTACTTATGT CATTAAAAAT
    GGAAATCCCT TACTAGGACA AATTACCGCT TCGGGTTGTT CATTGGGAAG TGTGATGGGT
    GTAACGGCTT CTATTTGTCA AAATGACAAA TTGTTGGCAG CAATTACTGC AACTCTACTT
    TATAATATAG CTTCTGAATT AGCAGTGGAA GCGAAAAACT CATGTGGTGA CTTACTCGTT
    CAAGGTCCTG GTACTTTTAT TCCCATTTTT GTTGATAAGC TACATCAATT AATTAATGAG
    ACTATAAAAG GAAATGTTGA TTGGATTGAA CGTGCCAAAT TAGAGAAAGC TGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593396.1
    CDS358..1914
    Translation

    Target ORF information:

    RefSeq Version NM_001018828.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase (thi4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001018828.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAACGTC AAATTGACTA TTCTTTATAC CTTGTAACTA GCTCTAGTTT AATTGCCCCA 
    GGTTCTACTA TAGAACGTCA GGTGGAAGAG GGAATTTTGG GAGGTGTCAC GCTTGTTCAG
    CACAGAGAAA AAGACATCTC TACTAAATGC TTTGTAGAAC GTGCTAAACG ACTTTCTGAA
    ATTTGCAAAA AGTACGACGT GCCTTTTCTA ATTAATGATA GGATTGACGT GGCATTAGCT
    GTTGGCGCTG ACGGTGTTCA TATTGGTCAA GACGACATGG ATTGTGCATT AGCCAGAAAA
    ATTCTTGGCG ATGATGCTAT TATTGGAGTG TCCACTAATA ATATCGAGGA AATTGAAAAA
    GCAGCTGCTG ATGGGGCCGA TTATGTGGGT ATCGGTTCTA TTTACGAGAC AAACACTAAA
    GATGTCAAAG ACAGGTTAAT AGGAATCACA GGTCTGCGAA AAATTCTTGA ACATGTTTCT
    AAAATGCATT GTCAACTTGG TACAGTGGCA ATTGCTGGTT TGAACTCATC GAATATCCAA
    AGGGTCATTT ATTTGAGCGA AGCCAATGGA AAAAGAATTG ACGGAATTGC TCTTGTTTCT
    GCTATCATGT GCTCCATTAC TCCTAGAGAA ACCGCTAAAG AACTCCGTAA CCTTATAGCC
    ACTCCTCCAT GCTTTGCTCA AGCACGTTCG TCTCTTACAA CTCCCAAGGA TTTACTGAAC
    CAAATTCCTG CTGCTTTACA GAAGTTGAAG GATTTCACTC CTCTAATTCA TCATCTGACT
    AATGCTGTTG CAAAAAACTT TTCTGCTAAC GTTACTCTTG CAGCATATGG TTCTCCAACT
    ATGGGTGAAA GCTACGATGA AGTTGCTGAT TTTGCAAAAG CCCCAGGTGC TCTTGTATTG
    AATATTGGAA TTCTTGAAAA TACAAAGACG TATATTCATG CTGCTCAAGT CAACAATGAT
    TTAGCCAGGC CCGTTATTTT AGATCCAGTT GCCGTAGGTG CTACAACCGC TCGTTCAAAA
    GTTATTAATA CTTTGTTAAA TTATGCTTAC TATGATATTA TCAAAGGGAA TGAAGGAGAA
    ATTATGAACT TAGCAGGTGA GCAAGGTTTG ATGAGAGGAG TAGATAGTAT TTCCCAGCAT
    ACATTAGCTG CTCGCATTAC AGCTGTTCAC CGCCTTGCTG TTGAAAGACG CTGTGTAGTA
    GCAATGTCTG GTGCAGTTGA TGTTATCAGC GATGGAAATT CTACTTATGT CATTAAAAAT
    GGAAATCCCT TACTAGGACA AATTACCGCT TCGGGTTGTT CATTGGGAAG TGTGATGGGT
    GTAACGGCTT CTATTTGTCA AAATGACAAA TTGTTGGCAG CAATTACTGC AACTCTACTT
    TATAATATAG CTTCTGAATT AGCAGTGGAA GCGAAAAACT CATGTGGTGA CTTACTCGTT
    CAAGGTCCTG GTACTTTTAT TCCCATTTTT GTTGATAAGC TACATCAATT AATTAATGAG
    ACTATAAAAG GAAATGTTGA TTGGATTGAA CGTGCCAAAT TAGAGAAAGC TGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L