ORF » Species Summary » Schizosaccharomyces pombe 972h- » MTR4 cDNA ORF clone

MTR4 cDNA ORF clone, Schizosaccharomyces pombe 972h-

  • Gene

  • Clones

  • gRNAs

The following MTR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102309 NM_001356009.1
Latest version!		 Schizosaccharomyces pombe TRAMP complex ATP-dependent RNA helicase subunit Mtr4 (mtr4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OSj02891 NM_001018732.2
Latest version!		 Schizosaccharomyces pombe 972h- ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 (mtr4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MTR4 ( NM_001356009.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342839.1 Schizosaccharomyces pombe TRAMP complex ATP-dependent RNA helicase subunit Mtr4 (mtr4), mRNA.
    Price & Availability↑
    CloneID OSc102309
    Clone ID Related Accession (Same CDS sequence) NM_001356009.1
    Accession Version NM_001356009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product TRAMP complex ATP-dependent RNA helicase subunit Mtr4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTTGGTG GTGAGTTAGA TGATGCTTTT GGAGTCTTTG AAGGTAAAGT TCCAAAAAGC 
    TTAAAGGAGG AATCTAAAAA TTCACAAAAT TCACAAAATT CACAAAAAAT AAAGCGTACT 
    TTAACAGATA AAAATGCCTC TAACCAAGAG CAAGGTACAA AAAAGTTGGA GTCTTCAGTA 
    GGGGAACAAG AATCTGCAAC TAAAAGAGCT AAAATCGAAA ATCTGAAGGA TAACCAAGAC 
    CTTATTCCTA ACAATGATGT TAACGGAATT CATATTAACA ATTCTGCAGT CGCTGATACC 
    AAACATAAGC CAAAAATAGG AGACATTGCT GCTGATGATA TTTCCAATGA AGTATCTATA 
    AAAAACGAAG GCGATACTAT ACCTGAGGCT ACGGTAGCTG ATTCTTTTGA GCAGGAAGCA 
    TCATTACAAG TTGCTGGGAA GGTTGGGATG ACCGAAGCTA AATCATCTAC TGAAGAAGTT 
    GTAGAACTTC GTCATCAGGT CCGTCATCAA GTCTCTATTC CTCCAAATTA TGATTATGTA 
    CCTATCTCTA AACATAAAAG TCCCATACCC CCTGCGCGCA CATATCCCTT CACTTTGGAT 
    CCTTTTCAAG CAGTGTCTAT TGCCTGTATT GAGCGTCAAG AATCAGTTTT GGTCAGTGCT 
    CATACATCTG CTGGTAAAAC TGTTGTTGCA GAATATGCAG TTGCTCAATC GCTTCGTGAT 
    AAACAGAGAG TTATTTATAC AAGTCCTATC AAAGCTTTAA GTAATCAAAA GTATCGAGAA 
    CTTCTTGCTG AATTTGGAGA TGTCGGTCTT ATGACTGGTG ATGTTACTAT TAATCCAGAT 
    GCTACTTGTT TGGTTATGAC CACTGAAATT CTCCGTTCAA TGTTGTATCG TGGTTCAGAG 
    GTTATGCGTG AAGTTGCGTG GGTTATTTTT GATGAGATTC ACTACATGCG TGACAAAGAG 
    CGCGGTGTTG TTTGGGAAGA GACCATTATC TTATTACCCG ACAAATCACA TTTTGTCTTC 
    CTGTCTGCTA CAATTCCAAA TGCTATGCAA TTTGCTGAAT GGATTACAAA AATTCATCGT 
    CAGCCTTGTC ATGTAGTTTA TACAGATTTT CGACCTACGC CTCTTCAACA TTACCTATTC 
    CCTAGCGGTA GCGATGGCAT TCATTTAGTG GTTGATGAGA AAAGTAATTT CCGAGAAGAA 
    AACTTTCAAA GAGCAATGAG TGCATTGATG GAAAAACAAG GCGATGACCC TGCCGCTATG 
    GCTACTAAAG GTAATGCAAA GAAAGGAAAG ACCGGAAAAG GCGGTGTCAA AGGACCTTCT 
    GATATTTACA AAATCGTAAA AATGATTATG GTGAAAAATT ATAACCCGGT TATTGTATTT 
    AGCTTTAGCA AAAGAGAATG TGAAGCTTTG GCCTTGCAAA TGTCAAAGTT AGACATGAAC 
    GACCAAACTG AGCGAGATTT GGTCACCACG ATTTTTAATA ATGCAGTGAA CCAATTATCG 
    GAGAAGGATA GAGAGTTACC ACAAATAGAG CATATTTTAC CTCTTCTTAG ACGTGGTATT 
    GGTATTCATC ATTCTGGTTT ATTGCCAATT TTGAAAGAAG TCATCGAGAT TTTGTTTCAG 
    GAAGGCCTTT TAAAGGTTTT GTTCGCTACA GAAACATTTT CTATTGGACT AAATATGCCT 
    GCAAAAACTG TCGTATTTAC AAACGTTCGA AAATTCGATG GTAAAACTTT TCGTTGGATA 
    TCAGGTGGTG AATACATTCA AATGAGCGGT CGTGCTGGTC GTCGTGGTTT GGATGACCGT 
    GGTATTGTAA TTTTAATGAT TGACGAAAAA ATGGATCCGC CTGTTGCAAA AAGTATGCTT 
    AAAGGTGAAG CTGACCGATT AGATTCTGCA TTCCACTTAA GCTACAACAT GATTCTTAAC 
    CTATTGCGAG TAGAGGGAAT TTCCCCCGAG TTCATGCTGG AAAGATGTTT CTTCCAATTT 
    CAGAACAGTT TAGAGGTTCC AAAATTGGAG GCAAAGTTAG AGGAGTCTCA GCAACATTAT 
    GATTCTTTTA CAATACTTGA TGAGCGCCCC TTGGAAGAAT ATCACACATT AAAAACTCAA 
    TTGGAAAGAT ATCGAACAGA TGTTCGTACT GTCGTCAACC ACCCCAACTT TTGTCTTTCT 
    TTTTTGCAAG GAGGTCGCCT TGTGCGTGTT AAAGTTGGAA ATGAAGACTT TGATTGGGGC 
    GTCGTCGTAA ATGTTTCTAA GCGACCACTT CCTAAAGGGC AGTCAAATGA GTACTTACCC 
    CAAGAATCAT ATATAGTACA TACTCTTGTA ATGGTGGCTA GCGACACAGG GCCATTGCGT 
    ATTAGAAGTG GTCATCTGCC TGAAGTGCAT CCACCTGCTG CAGAGGACAA AGGGAAATTT 
    GAAGTTGTTC CCTTTTTATT GTCCTCTTTA GATGGTATTG CCCATATTCG CGTATTTTTA 
    CCTAATGATT TAAAGTCTCA AGGTCAAAAG CTTACAGTCG GGAAAGCCTT ATCGGAGGTC 
    AAAAGACGCT TTCCTGAAGG AATTACTTTA CTAGATCCTG TGGAAAATAT GAACATTAAG 
    GAACCTACTT TTATTAAGCT TATGAAGAAA GTAAATATTT TAGAGTCAAG ATTGTTGTCA 
    AATCCATTGC ATAATTTTTC TGAATTGGAA GAAAAATACG CCGAGTATTT GAGAAAATTA 
    GCCTTACTCG AAGAGGTTAA AGACCTAAAG AAAAAACTTT CCAAAGCTCG CTCGATTATG 
    CAATTAGATG AGCTCAATAG TCGTAAGCGT GTTCTACGTC GTCTCGGATT TACCACTTCT 
    GACGACGTTA TTGAAGTTAA GGGCCGTGTA GCTTGCGAGA TAAGTTCCGG TGATGGTTTA 
    TTATTAACTG AGCTGATCTT CAACGGTATG TTTAACGATT TGACCCCAGA ACAATGTGCT 
    GCTCTCCTAA GTTGCTTGGT GTTTCAAGAA AAGAGTGAAG TAGAAAACCA AAGAATGAAA 
    GAAGAGCTTG CCGGTCCTTT GAAAATCTTG CAAGAGATGG CACGTCGGAT TGCTAAAGTT 
    TCCAAGGAAA GTAAGCAAGA ACTTAATGAA GAGGAATATG TTAATAGTTT TAAGCCATCT 
    CTAATGGAGG TTGTTTATGC TTGGGCACAT GGAGCCTCTT TTGCTCAAAT CTGCAAAATG 
    ACTGATGTTT ATGAAGGTTC ACTGATCCGC ATGTTCAGAA GGCTTGAAGA ACTTATACGA 
    CAAATGGTTG ATGCTGCGAA GGTTATAGGT AATACTTCTT TGCAGCAAAA AATGGAAGAC 
    ACTATCGCAT GCATACATCG GGATATTGTT TTTAGTGCAT CACTATATTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342839.1
    CDS110..3463
    Translation

    Target ORF information:

    RefSeq Version NM_001356009.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe TRAMP complex ATP-dependent RNA helicase subunit Mtr4 (mtr4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001356009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTTGGTG GTGAGTTAGA TGATGCTTTT GGAGTCTTTG AAGGTAAAGT TCCAAAAAGC 
    TTAAAGGAGG AATCTAAAAA TTCACAAAAT TCACAAAATT CACAAAAAAT AAAGCGTACT 
    TTAACAGATA AAAATGCCTC TAACCAAGAG CAAGGTACAA AAAAGTTGGA GTCTTCAGTA 
    GGGGAACAAG AATCTGCAAC TAAAAGAGCT AAAATCGAAA ATCTGAAGGA TAACCAAGAC 
    CTTATTCCTA ACAATGATGT TAACGGAATT CATATTAACA ATTCTGCAGT CGCTGATACC 
    AAACATAAGC CAAAAATAGG AGACATTGCT GCTGATGATA TTTCCAATGA AGTATCTATA 
    AAAAACGAAG GCGATACTAT ACCTGAGGCT ACGGTAGCTG ATTCTTTTGA GCAGGAAGCA 
    TCATTACAAG TTGCTGGGAA GGTTGGGATG ACCGAAGCTA AATCATCTAC TGAAGAAGTT 
    GTAGAACTTC GTCATCAGGT CCGTCATCAA GTCTCTATTC CTCCAAATTA TGATTATGTA 
    CCTATCTCTA AACATAAAAG TCCCATACCC CCTGCGCGCA CATATCCCTT CACTTTGGAT 
    CCTTTTCAAG CAGTGTCTAT TGCCTGTATT GAGCGTCAAG AATCAGTTTT GGTCAGTGCT 
    CATACATCTG CTGGTAAAAC TGTTGTTGCA GAATATGCAG TTGCTCAATC GCTTCGTGAT 
    AAACAGAGAG TTATTTATAC AAGTCCTATC AAAGCTTTAA GTAATCAAAA GTATCGAGAA 
    CTTCTTGCTG AATTTGGAGA TGTCGGTCTT ATGACTGGTG ATGTTACTAT TAATCCAGAT 
    GCTACTTGTT TGGTTATGAC CACTGAAATT CTCCGTTCAA TGTTGTATCG TGGTTCAGAG 
    GTTATGCGTG AAGTTGCGTG GGTTATTTTT GATGAGATTC ACTACATGCG TGACAAAGAG 
    CGCGGTGTTG TTTGGGAAGA GACCATTATC TTATTACCCG ACAAATCACA TTTTGTCTTC 
    CTGTCTGCTA CAATTCCAAA TGCTATGCAA TTTGCTGAAT GGATTACAAA AATTCATCGT 
    CAGCCTTGTC ATGTAGTTTA TACAGATTTT CGACCTACGC CTCTTCAACA TTACCTATTC 
    CCTAGCGGTA GCGATGGCAT TCATTTAGTG GTTGATGAGA AAAGTAATTT CCGAGAAGAA 
    AACTTTCAAA GAGCAATGAG TGCATTGATG GAAAAACAAG GCGATGACCC TGCCGCTATG 
    GCTACTAAAG GTAATGCAAA GAAAGGAAAG ACCGGAAAAG GCGGTGTCAA AGGACCTTCT 
    GATATTTACA AAATCGTAAA AATGATTATG GTGAAAAATT ATAACCCGGT TATTGTATTT 
    AGCTTTAGCA AAAGAGAATG TGAAGCTTTG GCCTTGCAAA TGTCAAAGTT AGACATGAAC 
    GACCAAACTG AGCGAGATTT GGTCACCACG ATTTTTAATA ATGCAGTGAA CCAATTATCG 
    GAGAAGGATA GAGAGTTACC ACAAATAGAG CATATTTTAC CTCTTCTTAG ACGTGGTATT 
    GGTATTCATC ATTCTGGTTT ATTGCCAATT TTGAAAGAAG TCATCGAGAT TTTGTTTCAG 
    GAAGGCCTTT TAAAGGTTTT GTTCGCTACA GAAACATTTT CTATTGGACT AAATATGCCT 
    GCAAAAACTG TCGTATTTAC AAACGTTCGA AAATTCGATG GTAAAACTTT TCGTTGGATA 
    TCAGGTGGTG AATACATTCA AATGAGCGGT CGTGCTGGTC GTCGTGGTTT GGATGACCGT 
    GGTATTGTAA TTTTAATGAT TGACGAAAAA ATGGATCCGC CTGTTGCAAA AAGTATGCTT 
    AAAGGTGAAG CTGACCGATT AGATTCTGCA TTCCACTTAA GCTACAACAT GATTCTTAAC 
    CTATTGCGAG TAGAGGGAAT TTCCCCCGAG TTCATGCTGG AAAGATGTTT CTTCCAATTT 
    CAGAACAGTT TAGAGGTTCC AAAATTGGAG GCAAAGTTAG AGGAGTCTCA GCAACATTAT 
    GATTCTTTTA CAATACTTGA TGAGCGCCCC TTGGAAGAAT ATCACACATT AAAAACTCAA 
    TTGGAAAGAT ATCGAACAGA TGTTCGTACT GTCGTCAACC ACCCCAACTT TTGTCTTTCT 
    TTTTTGCAAG GAGGTCGCCT TGTGCGTGTT AAAGTTGGAA ATGAAGACTT TGATTGGGGC 
    GTCGTCGTAA ATGTTTCTAA GCGACCACTT CCTAAAGGGC AGTCAAATGA GTACTTACCC 
    CAAGAATCAT ATATAGTACA TACTCTTGTA ATGGTGGCTA GCGACACAGG GCCATTGCGT 
    ATTAGAAGTG GTCATCTGCC TGAAGTGCAT CCACCTGCTG CAGAGGACAA AGGGAAATTT 
    GAAGTTGTTC CCTTTTTATT GTCCTCTTTA GATGGTATTG CCCATATTCG CGTATTTTTA 
    CCTAATGATT TAAAGTCTCA AGGTCAAAAG CTTACAGTCG GGAAAGCCTT ATCGGAGGTC 
    AAAAGACGCT TTCCTGAAGG AATTACTTTA CTAGATCCTG TGGAAAATAT GAACATTAAG 
    GAACCTACTT TTATTAAGCT TATGAAGAAA GTAAATATTT TAGAGTCAAG ATTGTTGTCA 
    AATCCATTGC ATAATTTTTC TGAATTGGAA GAAAAATACG CCGAGTATTT GAGAAAATTA 
    GCCTTACTCG AAGAGGTTAA AGACCTAAAG AAAAAACTTT CCAAAGCTCG CTCGATTATG 
    CAATTAGATG AGCTCAATAG TCGTAAGCGT GTTCTACGTC GTCTCGGATT TACCACTTCT 
    GACGACGTTA TTGAAGTTAA GGGCCGTGTA GCTTGCGAGA TAAGTTCCGG TGATGGTTTA 
    TTATTAACTG AGCTGATCTT CAACGGTATG TTTAACGATT TGACCCCAGA ACAATGTGCT 
    GCTCTCCTAA GTTGCTTGGT GTTTCAAGAA AAGAGTGAAG TAGAAAACCA AAGAATGAAA 
    GAAGAGCTTG CCGGTCCTTT GAAAATCTTG CAAGAGATGG CACGTCGGAT TGCTAAAGTT 
    TCCAAGGAAA GTAAGCAAGA ACTTAATGAA GAGGAATATG TTAATAGTTT TAAGCCATCT 
    CTAATGGAGG TTGTTTATGC TTGGGCACAT GGAGCCTCTT TTGCTCAAAT CTGCAAAATG 
    ACTGATGTTT ATGAAGGTTC ACTGATCCGC ATGTTCAGAA GGCTTGAAGA ACTTATACGA 
    CAAATGGTTG ATGCTGCGAA GGTTATAGGT AATACTTCTT TGCAGCAAAA AATGGAAGAC 
    ACTATCGCAT GCATACATCG GGATATTGTT TTTAGTGCAT CACTATATTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MTR4 ( NM_001018732.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_593302.1 Schizosaccharomyces pombe 972h- ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 (mtr4), mRNA.
    Price & Availability↑
    CloneID OSj02891
    Clone ID Related Accession (Same CDS sequence) NM_001018732.2
    Accession Version NM_001018732.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product ATP-dependent RNA helicase, TRAMP complex subunit Mtr4
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018732.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTTGGTG GTGAGTTAGA TGATGCTTTT GGAGTCTTTG AAGGTAAAGT TCCAAAAAGC 
    TTAAAGGAGG AATCTAAAAA TTCACAAAAT TCACAAAATT CACAAAAAAT AAAGCGTACT 
    TTAACAGATA AAAATGCCTC TAACCAAGAG CAAGGTACAA AAAAGTTGGA GTCTTCAGTA 
    GGGGAACAAG AATCTGCAAC TAAAAGAGCT AAAATCGAAA ATCTGAAGGA TAACCAAGAC 
    CTTATTCCTA ACAATGATGT TAACGGAATT CATATTAACA ATTCTGCAGT CGCTGATACC 
    AAACATAAGC CAAAAATAGG AGACATTGCT GCTGATGATA TTTCCAATGA AGTATCTATA 
    AAAAACGAAG GCGATACTAT ACCTGAGGCT ACGGTAGCTG ATTCTTTTGA GCAGGAAGCA 
    TCATTACAAG TTGCTGGGAA GGTTGGGATG ACCGAAGCTA AATCATCTAC TGAAGAAGTT 
    GTAGAACTTC GTCATCAGGT CCGTCATCAA GTCTCTATTC CTCCAAATTA TGATTATGTA 
    CCTATCTCTA AACATAAAAG TCCCATACCC CCTGCGCGCA CATATCCCTT CACTTTGGAT 
    CCTTTTCAAG CAGTGTCTAT TGCCTGTATT GAGCGTCAAG AATCAGTTTT GGTCAGTGCT 
    CATACATCTG CTGGTAAAAC TGTTGTTGCA GAATATGCAG TTGCTCAATC GCTTCGTGAT 
    AAACAGAGAG TTATTTATAC AAGTCCTATC AAAGCTTTAA GTAATCAAAA GTATCGAGAA 
    CTTCTTGCTG AATTTGGAGA TGTCGGTCTT ATGACTGGTG ATGTTACTAT TAATCCAGAT 
    GCTACTTGTT TGGTTATGAC CACTGAAATT CTCCGTTCAA TGTTGTATCG TGGTTCAGAG 
    GTTATGCGTG AAGTTGCGTG GGTTATTTTT GATGAGATTC ACTACATGCG TGACAAAGAG 
    CGCGGTGTTG TTTGGGAAGA GACCATTATC TTATTACCCG ACAAATCACA TTTTGTCTTC 
    CTGTCTGCTA CAATTCCAAA TGCTATGCAA TTTGCTGAAT GGATTACAAA AATTCATCGT 
    CAGCCTTGTC ATGTAGTTTA TACAGATTTT CGACCTACGC CTCTTCAACA TTACCTATTC 
    CCTAGCGGTA GCGATGGCAT TCATTTAGTG GTTGATGAGA AAAGTAATTT CCGAGAAGAA 
    AACTTTCAAA GAGCAATGAG TGCATTGATG GAAAAACAAG GCGATGACCC TGCCGCTATG 
    GCTACTAAAG GTAATGCAAA GAAAGGAAAG ACCGGAAAAG GCGGTGTCAA AGGACCTTCT 
    GATATTTACA AAATCGTAAA AATGATTATG GTGAAAAATT ATAACCCGGT TATTGTATTT 
    AGCTTTAGCA AAAGAGAATG TGAAGCTTTG GCCTTGCAAA TGTCAAAGTT AGACATGAAC 
    GACCAAACTG AGCGAGATTT GGTCACCACG ATTTTTAATA ATGCAGTGAA CCAATTATCG 
    GAGAAGGATA GAGAGTTACC ACAAATAGAG CATATTTTAC CTCTTCTTAG ACGTGGTATT 
    GGTATTCATC ATTCTGGTTT ATTGCCAATT TTGAAAGAAG TCATCGAGAT TTTGTTTCAG 
    GAAGGCCTTT TAAAGGTTTT GTTCGCTACA GAAACATTTT CTATTGGACT AAATATGCCT 
    GCAAAAACTG TCGTATTTAC AAACGTTCGA AAATTCGATG GTAAAACTTT TCGTTGGATA 
    TCAGGTGGTG AATACATTCA AATGAGCGGT CGTGCTGGTC GTCGTGGTTT GGATGACCGT 
    GGTATTGTAA TTTTAATGAT TGACGAAAAA ATGGATCCGC CTGTTGCAAA AAGTATGCTT 
    AAAGGTGAAG CTGACCGATT AGATTCTGCA TTCCACTTAA GCTACAACAT GATTCTTAAC 
    CTATTGCGAG TAGAGGGAAT TTCCCCCGAG TTCATGCTGG AAAGATGTTT CTTCCAATTT 
    CAGAACAGTT TAGAGGTTCC AAAATTGGAG GCAAAGTTAG AGGAGTCTCA GCAACATTAT 
    GATTCTTTTA CAATACTTGA TGAGCGCCCC TTGGAAGAAT ATCACACATT AAAAACTCAA 
    TTGGAAAGAT ATCGAACAGA TGTTCGTACT GTCGTCAACC ACCCCAACTT TTGTCTTTCT 
    TTTTTGCAAG GAGGTCGCCT TGTGCGTGTT AAAGTTGGAA ATGAAGACTT TGATTGGGGC 
    GTCGTCGTAA ATGTTTCTAA GCGACCACTT CCTAAAGGGC AGTCAAATGA GTACTTACCC 
    CAAGAATCAT ATATAGTACA TACTCTTGTA ATGGTGGCTA GCGACACAGG GCCATTGCGT 
    ATTAGAAGTG GTCATCTGCC TGAAGTGCAT CCACCTGCTG CAGAGGACAA AGGGAAATTT 
    GAAGTTGTTC CCTTTTTATT GTCCTCTTTA GATGGTATTG CCCATATTCG CGTATTTTTA 
    CCTAATGATT TAAAGTCTCA AGGTCAAAAG CTTACAGTCG GGAAAGCCTT ATCGGAGGTC 
    AAAAGACGCT TTCCTGAAGG AATTACTTTA CTAGATCCTG TGGAAAATAT GAACATTAAG 
    GAACCTACTT TTATTAAGCT TATGAAGAAA GTAAATATTT TAGAGTCAAG ATTGTTGTCA 
    AATCCATTGC ATAATTTTTC TGAATTGGAA GAAAAATACG CCGAGTATTT GAGAAAATTA 
    GCCTTACTCG AAGAGGTTAA AGACCTAAAG AAAAAACTTT CCAAAGCTCG CTCGATTATG 
    CAATTAGATG AGCTCAATAG TCGTAAGCGT GTTCTACGTC GTCTCGGATT TACCACTTCT 
    GACGACGTTA TTGAAGTTAA GGGCCGTGTA GCTTGCGAGA TAAGTTCCGG TGATGGTTTA 
    TTATTAACTG AGCTGATCTT CAACGGTATG TTTAACGATT TGACCCCAGA ACAATGTGCT 
    GCTCTCCTAA GTTGCTTGGT GTTTCAAGAA AAGAGTGAAG TAGAAAACCA AAGAATGAAA 
    GAAGAGCTTG CCGGTCCTTT GAAAATCTTG CAAGAGATGG CACGTCGGAT TGCTAAAGTT 
    TCCAAGGAAA GTAAGCAAGA ACTTAATGAA GAGGAATATG TTAATAGTTT TAAGCCATCT 
    CTAATGGAGG TTGTTTATGC TTGGGCACAT GGAGCCTCTT TTGCTCAAAT CTGCAAAATG 
    ACTGATGTTT ATGAAGGTTC ACTGATCCGC ATGTTCAGAA GGCTTGAAGA ACTTATACGA 
    CAAATGGTTG ATGCTGCGAA GGTTATAGGT AATACTTCTT TGCAGCAAAA AATGGAAGAC 
    ACTATCGCAT GCATACATCG GGATATTGTT TTTAGTGCAT CACTATATTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593302.1
    CDS173..3526
    Translation

    Target ORF information:

    RefSeq Version NM_001018732.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 (mtr4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTTGGTG GTGAGTTAGA TGATGCTTTT GGAGTCTTTG AAGGTAAAGT TCCAAAAAGC 
    TTAAAGGAGG AATCTAAAAA TTCACAAAAT TCACAAAATT CACAAAAAAT AAAGCGTACT 
    TTAACAGATA AAAATGCCTC TAACCAAGAG CAAGGTACAA AAAAGTTGGA GTCTTCAGTA 
    GGGGAACAAG AATCTGCAAC TAAAAGAGCT AAAATCGAAA ATCTGAAGGA TAACCAAGAC 
    CTTATTCCTA ACAATGATGT TAACGGAATT CATATTAACA ATTCTGCAGT CGCTGATACC 
    AAACATAAGC CAAAAATAGG AGACATTGCT GCTGATGATA TTTCCAATGA AGTATCTATA 
    AAAAACGAAG GCGATACTAT ACCTGAGGCT ACGGTAGCTG ATTCTTTTGA GCAGGAAGCA 
    TCATTACAAG TTGCTGGGAA GGTTGGGATG ACCGAAGCTA AATCATCTAC TGAAGAAGTT 
    GTAGAACTTC GTCATCAGGT CCGTCATCAA GTCTCTATTC CTCCAAATTA TGATTATGTA 
    CCTATCTCTA AACATAAAAG TCCCATACCC CCTGCGCGCA CATATCCCTT CACTTTGGAT 
    CCTTTTCAAG CAGTGTCTAT TGCCTGTATT GAGCGTCAAG AATCAGTTTT GGTCAGTGCT 
    CATACATCTG CTGGTAAAAC TGTTGTTGCA GAATATGCAG TTGCTCAATC GCTTCGTGAT 
    AAACAGAGAG TTATTTATAC AAGTCCTATC AAAGCTTTAA GTAATCAAAA GTATCGAGAA 
    CTTCTTGCTG AATTTGGAGA TGTCGGTCTT ATGACTGGTG ATGTTACTAT TAATCCAGAT 
    GCTACTTGTT TGGTTATGAC CACTGAAATT CTCCGTTCAA TGTTGTATCG TGGTTCAGAG 
    GTTATGCGTG AAGTTGCGTG GGTTATTTTT GATGAGATTC ACTACATGCG TGACAAAGAG 
    CGCGGTGTTG TTTGGGAAGA GACCATTATC TTATTACCCG ACAAATCACA TTTTGTCTTC 
    CTGTCTGCTA CAATTCCAAA TGCTATGCAA TTTGCTGAAT GGATTACAAA AATTCATCGT 
    CAGCCTTGTC ATGTAGTTTA TACAGATTTT CGACCTACGC CTCTTCAACA TTACCTATTC 
    CCTAGCGGTA GCGATGGCAT TCATTTAGTG GTTGATGAGA AAAGTAATTT CCGAGAAGAA 
    AACTTTCAAA GAGCAATGAG TGCATTGATG GAAAAACAAG GCGATGACCC TGCCGCTATG 
    GCTACTAAAG GTAATGCAAA GAAAGGAAAG ACCGGAAAAG GCGGTGTCAA AGGACCTTCT 
    GATATTTACA AAATCGTAAA AATGATTATG GTGAAAAATT ATAACCCGGT TATTGTATTT 
    AGCTTTAGCA AAAGAGAATG TGAAGCTTTG GCCTTGCAAA TGTCAAAGTT AGACATGAAC 
    GACCAAACTG AGCGAGATTT GGTCACCACG ATTTTTAATA ATGCAGTGAA CCAATTATCG 
    GAGAAGGATA GAGAGTTACC ACAAATAGAG CATATTTTAC CTCTTCTTAG ACGTGGTATT 
    GGTATTCATC ATTCTGGTTT ATTGCCAATT TTGAAAGAAG TCATCGAGAT TTTGTTTCAG 
    GAAGGCCTTT TAAAGGTTTT GTTCGCTACA GAAACATTTT CTATTGGACT AAATATGCCT 
    GCAAAAACTG TCGTATTTAC AAACGTTCGA AAATTCGATG GTAAAACTTT TCGTTGGATA 
    TCAGGTGGTG AATACATTCA AATGAGCGGT CGTGCTGGTC GTCGTGGTTT GGATGACCGT 
    GGTATTGTAA TTTTAATGAT TGACGAAAAA ATGGATCCGC CTGTTGCAAA AAGTATGCTT 
    AAAGGTGAAG CTGACCGATT AGATTCTGCA TTCCACTTAA GCTACAACAT GATTCTTAAC 
    CTATTGCGAG TAGAGGGAAT TTCCCCCGAG TTCATGCTGG AAAGATGTTT CTTCCAATTT 
    CAGAACAGTT TAGAGGTTCC AAAATTGGAG GCAAAGTTAG AGGAGTCTCA GCAACATTAT 
    GATTCTTTTA CAATACTTGA TGAGCGCCCC TTGGAAGAAT ATCACACATT AAAAACTCAA 
    TTGGAAAGAT ATCGAACAGA TGTTCGTACT GTCGTCAACC ACCCCAACTT TTGTCTTTCT 
    TTTTTGCAAG GAGGTCGCCT TGTGCGTGTT AAAGTTGGAA ATGAAGACTT TGATTGGGGC 
    GTCGTCGTAA ATGTTTCTAA GCGACCACTT CCTAAAGGGC AGTCAAATGA GTACTTACCC 
    CAAGAATCAT ATATAGTACA TACTCTTGTA ATGGTGGCTA GCGACACAGG GCCATTGCGT 
    ATTAGAAGTG GTCATCTGCC TGAAGTGCAT CCACCTGCTG CAGAGGACAA AGGGAAATTT 
    GAAGTTGTTC CCTTTTTATT GTCCTCTTTA GATGGTATTG CCCATATTCG CGTATTTTTA 
    CCTAATGATT TAAAGTCTCA AGGTCAAAAG CTTACAGTCG GGAAAGCCTT ATCGGAGGTC 
    AAAAGACGCT TTCCTGAAGG AATTACTTTA CTAGATCCTG TGGAAAATAT GAACATTAAG 
    GAACCTACTT TTATTAAGCT TATGAAGAAA GTAAATATTT TAGAGTCAAG ATTGTTGTCA 
    AATCCATTGC ATAATTTTTC TGAATTGGAA GAAAAATACG CCGAGTATTT GAGAAAATTA 
    GCCTTACTCG AAGAGGTTAA AGACCTAAAG AAAAAACTTT CCAAAGCTCG CTCGATTATG 
    CAATTAGATG AGCTCAATAG TCGTAAGCGT GTTCTACGTC GTCTCGGATT TACCACTTCT 
    GACGACGTTA TTGAAGTTAA GGGCCGTGTA GCTTGCGAGA TAAGTTCCGG TGATGGTTTA 
    TTATTAACTG AGCTGATCTT CAACGGTATG TTTAACGATT TGACCCCAGA ACAATGTGCT 
    GCTCTCCTAA GTTGCTTGGT GTTTCAAGAA AAGAGTGAAG TAGAAAACCA AAGAATGAAA 
    GAAGAGCTTG CCGGTCCTTT GAAAATCTTG CAAGAGATGG CACGTCGGAT TGCTAAAGTT 
    TCCAAGGAAA GTAAGCAAGA ACTTAATGAA GAGGAATATG TTAATAGTTT TAAGCCATCT 
    CTAATGGAGG TTGTTTATGC TTGGGCACAT GGAGCCTCTT TTGCTCAAAT CTGCAAAATG 
    ACTGATGTTT ATGAAGGTTC ACTGATCCGC ATGTTCAGAA GGCTTGAAGA ACTTATACGA 
    CAAATGGTTG ATGCTGCGAA GGTTATAGGT AATACTTCTT TGCAGCAAAA AATGGAAGAC 
    ACTATCGCAT GCATACATCG GGATATTGTT TTTAGTGCAT CACTATATTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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