rqh1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rqh1
Entrez Gene ID	2541620
Full Name	RecQ type DNA helicase Rqh1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018490.2	NP_593092.1	RecQ type DNA helicase Rqh1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Long G2 phase of pot1-rqh1 double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to thiabendazole.
Title: Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in Schizosaccharomyces pombe.

During replication-coupled DNA repair, CAF-1 promotes template switch by counteracting D-loop disassembly by Rqh1.
Title: The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.

Rqh1 is required for the maintenance of circular chromosomes.
Title: Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes.

Sumoylation of RecQ helicase controls the fate of dysfunctional telomeres.
Title: Sumoylation of RecQ helicase controls the fate of dysfunctional telomeres.

Removing Rhp51, the Rad51 recombinase homologue, suppresses the slowing defect of rqh1Delta mutants, but not that of mus81Delta mutant, defining an epistatic pathway in which mus81 is epistatic to rhp51 and rhp51 is epistatic to rqh1.
Title: Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

The following rqh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rqh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103625	NM_001018490.2 Latest version!	Schizosaccharomyces pombe RecQ type DNA helicase Rqh1 (rqh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rqh1 ( NM_001018490.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593092.1 Schizosaccharomyces pombe RecQ type DNA helicase Rqh1 (rqh1), mRNA.

Price & Availability↑

CloneID	OSc103625
Clone ID Related Accession (Same CDS sequence)	NM_001018490.2
Accession Version	NM_001018490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RecQ type DNA helicase Rqh1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018490.1.

ATGACAGTAA CGAAAACAAA CCTTAATCGT CATCTAGATT GGTTTTTTCG TGAAAGCCCT 
CAAAAAATCG AAAATGTCAC TAGCCCCATT AAAACATTGG ACTTCGTAAA AGTTAAAGTA 
TCTTCTTCTG ATATCGTGGT AAAGGATTCA ATACCTCATA AGAGCAAGAA CGTGTTTGAT 
GACTTTGATG ATGGTTATGC TATTGATTTA ACAGAAGAGC ATCAAAGCTC TTCATTGAAT 
AATTTAAAAT GGAAAGATGT TGAAGGACCC AATATCCTGA AACCAATCAA AAAAATTGCA 
GTTCCAGCTT CTGAAAGCGA AGAAGATTTT GATGATGTGG ATGAAGAAAT GTTAAGAGCA 
GCTGAGATGG AGGTTTTTCA AAGTTGCCAA CCATTGGCCG TTAATACTGC TGATACTACC 
GTTTCGCACT CAACATCTAG CTCAAACGTC CCTCGTTCCC TAAACAAAAT ACATGATCCT 
TCCAGATTTA TCAAAGATAA CGACGTTGAA AATAGGATTC ACGTTAGTAG TGCTTCAAAA 
GTTGCAAGTA TCAGCAATAC CTCTAAACCT AACCCAATCG TCAGCGAAAA TCCAATTTCT 
GCAACTTCGG TATCCATCGA AATCCCTATC AAACCAAAAG AGCTATCTAA TAACTTACCA 
TTCCCTCGTT TGAATAACAA CAACACTAAT AATAATAATG ATAATAATGC TATAGAGAAG 
AGGGATTCCG CTTCGCCTAC ACCTAGCAGT GTCTCGTCAC AAATTTCAAT TGATTTTAGT 
ACATGGCCTC ATCAAAATTT ATTGCAATAT CTGGATATAT TACGTGATGA AAAGAGTGAA 
ATCAGTGATA GAATTATAGA GGTTATGGAA AGATATCCTT TTTCTTCGCG ATTCAAAGAG 
TGGATTCCTA AGCGGGATAT TTTATCTCAA AAAATTTCTT CAGTACTTGA AGTGCTGTCC 
AACAACAATA ATAGTAATAA TAATAATGGA AATAATGGTA CGGTTCCTAA TGCTAAAACT 
TTTTTTACTC CTCCTTCCTC GATTACCCAA CAGGTGCCAT TCCCTTCTAC AATAATTCCG 
GAGTCTACCG TGAAAGAAAA TAGCACTCGG CCATATGTTA ATTCACATCT TGTTGCAAAT 
GATAAAATAA CAGCCACTCC TTTTCATTCG GAAGCAGTTG TCTCTCCTTT GCAAAGTAAT 
ATTCGCAATT CTGATATTGC TGAATTTGAT GAATTCGACA TCGACGATGC CGATTTTACC 
TTTAATACAA CGGATCCGAT TAATGATGAG TCAGGTGCCT CAAGTGATGT TGTTGTGATC 
GATGATGAAG AAGATGATAT TGAAAATAGA CCTTTAAACC AAGCTTTGAA AGCCAGTAAA 
GCTGCAGTTT CCAATGCTTC ACTTCTTCAA TCCAGTTCGC TTGATAGGCC GTTACTTGGT 
GAAATGAAGG ATAAAAACCA TAAAGTTTTG ATGCCATCTT TAGATGATCC CATGCTTTCG 
TATCCTTGGT CAAAAGAAGT CTTAGGTTGC TTGAAACATA AGTTTCATCT GAAAGGATTT 
CGTAAAAACC AACTTGAAGC AATTAATGGA ACGCTTTCTG GTAAAGATGT ATTCATTTTG 
ATGCCTACTG GAGGAGGTAA AAGTTTATGT TACCAGCTTC CTGCAGTTAT CGAAGGCGGA 
GCATCGCGAG GAGTCACACT CGTTATATCT CCTCTTCTTT CTTTAATGCA AGATCAACTT 
GATCACTTAA GAAAACTAAA TATACCTTCG CTACCGCTTA GTGGTGAGCA GCCTGCTGAT 
GAAAGAAGAC AGGTCATTTC GTTTCTTATG GCTAAAAACG TGTTGGTAAA GCTCCTTTAT 
GTAACGCCTG AGGGATTAGC TAGTAATGGG GCGATTACAA GGGTATTAAA AAGCTTATAT 
GAAAGGAAAC TTTTGGCTAG AATTGTGATT GACGAAGCTC ACTGCGTCAG TCACTGGGGC 
CATGATTTTC GACCAGATTA TAAGCAATTG GGCTTACTCA GAGATCGATA CCAAGGTATT 
CCTTTTATGG CGTTGACAGC CACTGCAAAT GAAATTGTCA AAAAAGATAT CATTAACACC 
TTGAGAATGG AGAACTGCTT GGAATTAAAG TCTAGTTTTA ATAGACCTAA TCTTTTTTAC 
GAAATCAAGC CCAAAAAAGA TCTTTACACC GAGTTATATC GCTTCATTAG CAACGGTCAT 
CTTCACGAAT CTGGTATTAT TTATTGCTTA TCTAGAACTA GTTGCGAACA AGTGGCAGCC 
AAACTTCGAA ATGACTATGG TTTGAAAGCA TGGCATTATC ACGCTGGTCT TGAAAAAGTT 
GAAAGGCAGA GAATTCAGAA TGAATGGCAA TCTGGTAGCT ATAAAATAAT TGTCGCAACA 
ATTGCATTTG GTATGGGAGT TGACAAAGGC GACGTTCGCT TTGTAATCCA CCACAGTTTT 
CCGAAATCCT TGGAAGGCTA TTATCAAGAA ACTGGGCGCG CTGGTCGTGA TGGAAAACCT 
GCGCATTGTA TTATGTTTTA TAGCTATAAG GACCACGTTA CATTTCAAAA GCTGATAATG 
AGCGGAGACG GAGATGCTGA GACTAAAGAA AGACAGCGTC AAATGCTTAG ACAAGTTATT 
CAGTTTTGCG AAAACAAAAC TGACTGCAGG AGAAAGCAAG TCCTTGCTTA TTTCGGTGAA 
AACTTTGATA AAGTGCACTG CAGAAAAGGA TGCGACATTT GCTGCGAAGA AGCAACATAC 
ATTAAACAGG ATATGACCGA ATTTTCACTG CAAGCAATTA AGTTATTGAA AAGTATTTCT 
GGGAAAGCAA CTCTTCTTCA ATTGATGGAT ATTTTTCGAG GCAGTAAAAG TGCCAAAATT 
GTGGAGAATG GATGGGACCG TTTGGAAGGC GCAGGAGTGG GTAAGCTTTT AAATCGTGGA 
GATAGTGAAC GTTTATTTCA TCATCTCGTT TCTGAAGGTG TATTTGTTGA GAAAGTTGAG 
GCAAACCGTC GAGGATTTGT CTCAGCTTAC GTTGTTCCCG GCAGACAAAC AATCATTAAT 
TCTGTACTTG CTGGCAAACG CAGGATAATT TTGGATGTCA AAGAGTCATC TTCAAAGCCG 
GACACGTCTA GTCGCTCACT TTCACGTAGT AAAACGCTGC CTGCTCTTCG AGAATATCAG 
TTGAAATCTA CTACTGCATC TGTTGATTGT TCCATTGGAA CACGTGAAGT AGATGAAATT 
TATGATTCTC AAATGCCACC GGTGAAACCC TCATTAATTC ACTCAAGAAA CAAGATTGAC 
TTAGAAGAGC TTTCAGGACA GAAATTTATG AGCGAATATG AGATAGATGT TATGACTCGC 
TGTCTAAAAG ACCTTAAACT TTTAAGAAGC AACTTAATGG CCATAGATGA CAGCAGAGTG 
TCTTCGTATT TCACAGATTC CGTATTACTA AGTATGGCCA AAAAGCTTCC CAGGAATGTA 
AAGGAATTAA AGGAAATACA CGGTGTAAGT AATGAAAAAG CTGTAAATTT AGGGCCTAAA 
TTTTTACAAG TGATTCAAAA ATTCATTGAC GAGAAAGAAC AAAATCTCGA GGGAACAGAG 
CTTGACCCTT CTCTGCAATC TCTGGATACT GATTACCCGA TTGACACCAA TGCTCTAAGT 
TTAGACCATG AGCAAGGGTT TTCTGATGAT AGCGACAGTG TTTACGAACC TTCTAGTCCT 
ATTGAAGAAG GGGATGAAGA AGTGGATGGT CAACGGAAAG ACATTTTAAA TTTTATGAAT 
TCTCAATCTT TAACGCAAAC TGGCTCAGTT CCTAAGCGTA AATCAACATC ATATACTCGA 
CCCTCTAAAT CATATCGCCA TAAGCGTGGT AGTACAAGTT ATAGTCGAAA AAGAAAGTAT 
TCGACTTCAC AAAAAGACTC TCGAAAAACT TCTAAAAGTG CCAACACATC ATTCATACAT 
CCCATGGTTA AGCAAAATTA TCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593092.1
CDS	110..4096
Translation	MTVTKTNLNRHLDWFFRESPQKIENVTSPIKTLDFVKVKVSSSDIVVKDSIPHKSKNVFDDFDDGYAIDLTEEHQSSSLNNLKWKDVEGPNILKPIKKIAVPASESEEDFDDVDEEMLRAAEMEVFQSCQPLAVNTADTTVSHSTSSSNVPRSLNKIHDPSRFIKDNDVENRIHVSSASKVASISNTSKPNPIVSENPISATSVSIEIPIKPKELSNNLPFPRLNNNNTNNNNDNNAIEKRDSASPTPSSVSSQISIDFSTWPHQNLLQYLDILRDEKSEISDRIIEVMERYPFSSRFKEWIPKRDILSQKISSVLEVLSNNNNSNNNNGNNGTVPNAKTFFTPPSSITQQVPFPSTIIPESTVKENSTRPYVNSHLVANDKITATPFHSEAVVSPLQSNIRNSDIAEFDEFDIDDADFTFNTTDPINDESGASSDVVVIDDEEDDIENRPLNQALKASKAAVSNASLLQSSSLDRPLLGEMKDKNHKVLMPSLDDPMLSYPWSKEVLGCLKHKFHLKGFRKNQLEAINGTLSGKDVFILMPTGGGKSLCYQLPAVIEGGASRGVTLVISPLLSLMQDQLDHLRKLNIPSLPLSGEQPADERRQVISFLMAKNVLVKLLYVTPEGLASNGAITRVLKSLYERKLLARIVIDEAHCVSHWGHDFRPDYKQLGLLRDRYQGIPFMALTATANEIVKKDIINTLRMENCLELKSSFNRPNLFYEIKPKKDLYTELYRFISNGHLHESGIIYCLSRTSCEQVAAKLRNDYGLKAWHYHAGLEKVERQRIQNEWQSGSYKIIVATIAFGMGVDKGDVRFVIHHSFPKSLEGYYQETGRAGRDGKPAHCIMFYSYKDHVTFQKLIMSGDGDAETKERQRQMLRQVIQFCENKTDCRRKQVLAYFGENFDKVHCRKGCDICCEEATYIKQDMTEFSLQAIKLLKSISGKATLLQLMDIFRGSKSAKIVENGWDRLEGAGVGKLLNRGDSERLFHHLVSEGVFVEKVEANRRGFVSAYVVPGRQTIINSVLAGKRRIILDVKESSSKPDTSSRSLSRSKTLPALREYQLKSTTASVDCSIGTREVDEIYDSQMPPVKPSLIHSRNKIDLEELSGQKFMSEYEIDVMTRCLKDLKLLRSNLMAIDDSRVSSYFTDSVLLSMAKKLPRNVKELKEIHGVSNEKAVNLGPKFLQVIQKFIDEKEQNLEGTELDPSLQSLDTDYPIDTNALSLDHEQGFSDDSDSVYEPSSPIEEGDEEVDGQRKDILNFMNSQSLTQTGSVPKRKSTSYTRPSKSYRHKRGSTSYSRKRKYSTSQKDSRKTSKSANTSFIHPMVKQNYR

Target ORF information:

RefSeq Version	NM_001018490.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RecQ type DNA helicase Rqh1 (rqh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018490.2

ORF Insert Sequence:

ATGACAGTAA CGAAAACAAA CCTTAATCGT CATCTAGATT GGTTTTTTCG TGAAAGCCCT 
CAAAAAATCG AAAATGTCAC TAGCCCCATT AAAACATTGG ACTTCGTAAA AGTTAAAGTA 
TCTTCTTCTG ATATCGTGGT AAAGGATTCA ATACCTCATA AGAGCAAGAA CGTGTTTGAT 
GACTTTGATG ATGGTTATGC TATTGATTTA ACAGAAGAGC ATCAAAGCTC TTCATTGAAT 
AATTTAAAAT GGAAAGATGT TGAAGGACCC AATATCCTGA AACCAATCAA AAAAATTGCA 
GTTCCAGCTT CTGAAAGCGA AGAAGATTTT GATGATGTGG ATGAAGAAAT GTTAAGAGCA 
GCTGAGATGG AGGTTTTTCA AAGTTGCCAA CCATTGGCCG TTAATACTGC TGATACTACC 
GTTTCGCACT CAACATCTAG CTCAAACGTC CCTCGTTCCC TAAACAAAAT ACATGATCCT 
TCCAGATTTA TCAAAGATAA CGACGTTGAA AATAGGATTC ACGTTAGTAG TGCTTCAAAA 
GTTGCAAGTA TCAGCAATAC CTCTAAACCT AACCCAATCG TCAGCGAAAA TCCAATTTCT 
GCAACTTCGG TATCCATCGA AATCCCTATC AAACCAAAAG AGCTATCTAA TAACTTACCA 
TTCCCTCGTT TGAATAACAA CAACACTAAT AATAATAATG ATAATAATGC TATAGAGAAG 
AGGGATTCCG CTTCGCCTAC ACCTAGCAGT GTCTCGTCAC AAATTTCAAT TGATTTTAGT 
ACATGGCCTC ATCAAAATTT ATTGCAATAT CTGGATATAT TACGTGATGA AAAGAGTGAA 
ATCAGTGATA GAATTATAGA GGTTATGGAA AGATATCCTT TTTCTTCGCG ATTCAAAGAG 
TGGATTCCTA AGCGGGATAT TTTATCTCAA AAAATTTCTT CAGTACTTGA AGTGCTGTCC 
AACAACAATA ATAGTAATAA TAATAATGGA AATAATGGTA CGGTTCCTAA TGCTAAAACT 
TTTTTTACTC CTCCTTCCTC GATTACCCAA CAGGTGCCAT TCCCTTCTAC AATAATTCCG 
GAGTCTACCG TGAAAGAAAA TAGCACTCGG CCATATGTTA ATTCACATCT TGTTGCAAAT 
GATAAAATAA CAGCCACTCC TTTTCATTCG GAAGCAGTTG TCTCTCCTTT GCAAAGTAAT 
ATTCGCAATT CTGATATTGC TGAATTTGAT GAATTCGACA TCGACGATGC CGATTTTACC 
TTTAATACAA CGGATCCGAT TAATGATGAG TCAGGTGCCT CAAGTGATGT TGTTGTGATC 
GATGATGAAG AAGATGATAT TGAAAATAGA CCTTTAAACC AAGCTTTGAA AGCCAGTAAA 
GCTGCAGTTT CCAATGCTTC ACTTCTTCAA TCCAGTTCGC TTGATAGGCC GTTACTTGGT 
GAAATGAAGG ATAAAAACCA TAAAGTTTTG ATGCCATCTT TAGATGATCC CATGCTTTCG 
TATCCTTGGT CAAAAGAAGT CTTAGGTTGC TTGAAACATA AGTTTCATCT GAAAGGATTT 
CGTAAAAACC AACTTGAAGC AATTAATGGA ACGCTTTCTG GTAAAGATGT ATTCATTTTG 
ATGCCTACTG GAGGAGGTAA AAGTTTATGT TACCAGCTTC CTGCAGTTAT CGAAGGCGGA 
GCATCGCGAG GAGTCACACT CGTTATATCT CCTCTTCTTT CTTTAATGCA AGATCAACTT 
GATCACTTAA GAAAACTAAA TATACCTTCG CTACCGCTTA GTGGTGAGCA GCCTGCTGAT 
GAAAGAAGAC AGGTCATTTC GTTTCTTATG GCTAAAAACG TGTTGGTAAA GCTCCTTTAT 
GTAACGCCTG AGGGATTAGC TAGTAATGGG GCGATTACAA GGGTATTAAA AAGCTTATAT 
GAAAGGAAAC TTTTGGCTAG AATTGTGATT GACGAAGCTC ACTGCGTCAG TCACTGGGGC 
CATGATTTTC GACCAGATTA TAAGCAATTG GGCTTACTCA GAGATCGATA CCAAGGTATT 
CCTTTTATGG CGTTGACAGC CACTGCAAAT GAAATTGTCA AAAAAGATAT CATTAACACC 
TTGAGAATGG AGAACTGCTT GGAATTAAAG TCTAGTTTTA ATAGACCTAA TCTTTTTTAC 
GAAATCAAGC CCAAAAAAGA TCTTTACACC GAGTTATATC GCTTCATTAG CAACGGTCAT 
CTTCACGAAT CTGGTATTAT TTATTGCTTA TCTAGAACTA GTTGCGAACA AGTGGCAGCC 
AAACTTCGAA ATGACTATGG TTTGAAAGCA TGGCATTATC ACGCTGGTCT TGAAAAAGTT 
GAAAGGCAGA GAATTCAGAA TGAATGGCAA TCTGGTAGCT ATAAAATAAT TGTCGCAACA 
ATTGCATTTG GTATGGGAGT TGACAAAGGC GACGTTCGCT TTGTAATCCA CCACAGTTTT 
CCGAAATCCT TGGAAGGCTA TTATCAAGAA ACTGGGCGCG CTGGTCGTGA TGGAAAACCT 
GCGCATTGTA TTATGTTTTA TAGCTATAAG GACCACGTTA CATTTCAAAA GCTGATAATG 
AGCGGAGACG GAGATGCTGA GACTAAAGAA AGACAGCGTC AAATGCTTAG ACAAGTTATT 
CAGTTTTGCG AAAACAAAAC TGACTGCAGG AGAAAGCAAG TCCTTGCTTA TTTCGGTGAA 
AACTTTGATA AAGTGCACTG CAGAAAAGGA TGCGACATTT GCTGCGAAGA AGCAACATAC 
ATTAAACAGG ATATGACCGA ATTTTCACTG CAAGCAATTA AGTTATTGAA AAGTATTTCT 
GGGAAAGCAA CTCTTCTTCA ATTGATGGAT ATTTTTCGAG GCAGTAAAAG TGCCAAAATT 
GTGGAGAATG GATGGGACCG TTTGGAAGGC GCAGGAGTGG GTAAGCTTTT AAATCGTGGA 
GATAGTGAAC GTTTATTTCA TCATCTCGTT TCTGAAGGTG TATTTGTTGA GAAAGTTGAG 
GCAAACCGTC GAGGATTTGT CTCAGCTTAC GTTGTTCCCG GCAGACAAAC AATCATTAAT 
TCTGTACTTG CTGGCAAACG CAGGATAATT TTGGATGTCA AAGAGTCATC TTCAAAGCCG 
GACACGTCTA GTCGCTCACT TTCACGTAGT AAAACGCTGC CTGCTCTTCG AGAATATCAG 
TTGAAATCTA CTACTGCATC TGTTGATTGT TCCATTGGAA CACGTGAAGT AGATGAAATT 
TATGATTCTC AAATGCCACC GGTGAAACCC TCATTAATTC ACTCAAGAAA CAAGATTGAC 
TTAGAAGAGC TTTCAGGACA GAAATTTATG AGCGAATATG AGATAGATGT TATGACTCGC 
TGTCTAAAAG ACCTTAAACT TTTAAGAAGC AACTTAATGG CCATAGATGA CAGCAGAGTG 
TCTTCGTATT TCACAGATTC CGTATTACTA AGTATGGCCA AAAAGCTTCC CAGGAATGTA 
AAGGAATTAA AGGAAATACA CGGTGTAAGT AATGAAAAAG CTGTAAATTT AGGGCCTAAA 
TTTTTACAAG TGATTCAAAA ATTCATTGAC GAGAAAGAAC AAAATCTCGA GGGAACAGAG 
CTTGACCCTT CTCTGCAATC TCTGGATACT GATTACCCGA TTGACACCAA TGCTCTAAGT 
TTAGACCATG AGCAAGGGTT TTCTGATGAT AGCGACAGTG TTTACGAACC TTCTAGTCCT 
ATTGAAGAAG GGGATGAAGA AGTGGATGGT CAACGGAAAG ACATTTTAAA TTTTATGAAT 
TCTCAATCTT TAACGCAAAC TGGCTCAGTT CCTAAGCGTA AATCAACATC ATATACTCGA 
CCCTCTAAAT CATATCGCCA TAAGCGTGGT AGTACAAGTT ATAGTCGAAA AAGAAAGTAT 
TCGACTTCAC AAAAAGACTC TCGAAAAACT TCTAAAAGTG CCAACACATC ATTCATACAT 
CCCATGGTTA AGCAAAATTA TCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rqh1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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