ORF » Species Summary » Schizosaccharomyces pombe » pda1 cDNA ORF clone

pda1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following pda1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pda1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103954 NM_001020321.2
Latest version!		 Schizosaccharomyces pombe putative pyruvate dehydrogenase e1 component alpha subunit Pda1 (pda1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pda1 ( NM_001020321.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594892.1 Schizosaccharomyces pombe putative pyruvate dehydrogenase e1 component alpha subunit Pda1 (pda1), mRNA.
    Price & Availability↑
    CloneID OSc103954
    Clone ID Related Accession (Same CDS sequence) NM_001020321.2
    Accession Version NM_001020321.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative pyruvate dehydrogenase e1 component alpha subunit Pda1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020321.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTTTCGAA CTTGTACGAA GATTGGAACA GTTCCCAAGG TTCTTGTGAA CCAAAAGGGC 
    TTGATCGATG GCCTTCGTCG GGTCACCACA GACGCAACCA CTTCTCGTGC CAATCCGGCT 
    CATGTGCCTG AGGAACATGA CAAGCCATTT CCTGTTAAAT TAGATGATAG TGTATTCGAA 
    GGATACAAGA TCGATGTCCC TTCTACTGAA ATCGAAGTTA CAAAGGGAGA GTTATTGGGT 
    TTGTACGAGA AGATGGTGAC TATTCGTCGT CTAGAACTTG CATGCGATGC CTTGTATAAG 
    GCTAAGAAGA TTCGTGGATT CTGTCATCTT AGCATTGGCC AAGAAGCTGT AGCTGCAGGA 
    ATTGAAGGTG CTATTACACT TGACGACAGT ATTATCACAT CTTATAGATG CCACGGTTTT 
    GCTTATACCC GTGGTTTGTC AATTCGAAGC ATTATTGGTG AGCTCATGGG ACGTCAATGT 
    GGTGCTTCCA AGGGCAAGGG TGGTTCTATG CACATTTTCG CCAAAAACTT CTATGGTGGT 
    AATGGTATTG TTGGTGCTCA AATTCCTTTG GGTGCTGGTA TTGGTTTCGC ACAGAAGTAT 
    CTTGAAAAAC CCACTACTAC TTTTGCTCTA TATGGTGATG GTGCATCTAA CCAAGGTCAA 
    GCTTTCGAGG CCTTCAACAT GGCCAAATTA TGGGGTCTTC CCGTTATTTT TGCTTGTGAA 
    AACAACAAAT ACGGTATGGG TACTAGTGCT GAACGCTCTT CTGCCATGAC TGAGTTCTAC 
    AAACGTGGAC AGTACATTCC CGGTCTTTTG GTTAACGGTA TGGATGTTTT GGCTGTTTTG 
    CAGGCTTCAA AGTTTGCTAA GAAGTACACT GTTGAAAACT CTCAACCTCT GCTTATGGAA 
    TTTGTGACTT ATCGTTATGG TGGTCACTCC ATGTCCGATC CCGGTACTAC TTATCGTAGC 
    CGTGAAGAAG TGCAAAAAGT ACGTGCTGCT AGAGATCCTA TTGAGGGTTT GAAGAAGCAC 
    ATCATGGAGT GGGGCGTCGC TAATGCCAAT GAGCTTAAAA ACATTGAGAA GAGAATCCGT 
    GGTATGGTTG ATGAGGAGGT TCGTATCGCT GAAGAAAGCC CTTTCCCCGA TCCTATTGAG 
    GAGAGTTTGT TTTCAGATGT TTACGTTGCA GGAACTGAAC CCGCTTACGC CCGTGGTAGA 
    AATTCCCTGG AATATCATCA ATATAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594892.1
    CDS249..1478
    Translation

    Target ORF information:

    RefSeq Version NM_001020321.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative pyruvate dehydrogenase e1 component alpha subunit Pda1 (pda1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001020321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTTTCGAA CTTGTACGAA GATTGGAACA GTTCCCAAGG TTCTTGTGAA CCAAAAGGGC 
    TTGATCGATG GCCTTCGTCG GGTCACCACA GACGCAACCA CTTCTCGTGC CAATCCGGCT 
    CATGTGCCTG AGGAACATGA CAAGCCATTT CCTGTTAAAT TAGATGATAG TGTATTCGAA 
    GGATACAAGA TCGATGTCCC TTCTACTGAA ATCGAAGTTA CAAAGGGAGA GTTATTGGGT 
    TTGTACGAGA AGATGGTGAC TATTCGTCGT CTAGAACTTG CATGCGATGC CTTGTATAAG 
    GCTAAGAAGA TTCGTGGATT CTGTCATCTT AGCATTGGCC AAGAAGCTGT AGCTGCAGGA 
    ATTGAAGGTG CTATTACACT TGACGACAGT ATTATCACAT CTTATAGATG CCACGGTTTT 
    GCTTATACCC GTGGTTTGTC AATTCGAAGC ATTATTGGTG AGCTCATGGG ACGTCAATGT 
    GGTGCTTCCA AGGGCAAGGG TGGTTCTATG CACATTTTCG CCAAAAACTT CTATGGTGGT 
    AATGGTATTG TTGGTGCTCA AATTCCTTTG GGTGCTGGTA TTGGTTTCGC ACAGAAGTAT 
    CTTGAAAAAC CCACTACTAC TTTTGCTCTA TATGGTGATG GTGCATCTAA CCAAGGTCAA 
    GCTTTCGAGG CCTTCAACAT GGCCAAATTA TGGGGTCTTC CCGTTATTTT TGCTTGTGAA 
    AACAACAAAT ACGGTATGGG TACTAGTGCT GAACGCTCTT CTGCCATGAC TGAGTTCTAC 
    AAACGTGGAC AGTACATTCC CGGTCTTTTG GTTAACGGTA TGGATGTTTT GGCTGTTTTG 
    CAGGCTTCAA AGTTTGCTAA GAAGTACACT GTTGAAAACT CTCAACCTCT GCTTATGGAA 
    TTTGTGACTT ATCGTTATGG TGGTCACTCC ATGTCCGATC CCGGTACTAC TTATCGTAGC 
    CGTGAAGAAG TGCAAAAAGT ACGTGCTGCT AGAGATCCTA TTGAGGGTTT GAAGAAGCAC 
    ATCATGGAGT GGGGCGTCGC TAATGCCAAT GAGCTTAAAA ACATTGAGAA GAGAATCCGT 
    GGTATGGTTG ATGAGGAGGT TCGTATCGCT GAAGAAAGCC CTTTCCCCGA TCCTATTGAG 
    GAGAGTTTGT TTTCAGATGT TTACGTTGCA GGAACTGAAC CCGCTTACGC CCGTGGTAGA 
    AATTCCCTGG AATATCATCA ATATAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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