ORF » Species Summary » Schizosaccharomyces pombe » SPAC227.09 cDNA ORF clone

SPAC227.09 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following SPAC227.09 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC227.09 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj02741 NM_001018363.2
Latest version!		 Schizosaccharomyces pombe 972h- folylpolyglutamate synthase (predicted) (SPAC227.09), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OSc103236 NM_001355981.1
Latest version!		 Schizosaccharomyces pombe putative folylpolyglutamate synthase (SPAC227.09), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPAC227.09 ( NM_001018363.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_592963.1 Schizosaccharomyces pombe 972h- folylpolyglutamate synthase (predicted) (SPAC227.09), mRNA.
    Price & Availability↑
    CloneID OSj02741
    Clone ID Related Accession (Same CDS sequence) NM_001018363.2
    Accession Version NM_001018363.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product folylpolyglutamate synthase (predicted)
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018363.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCCTATTC AGCTAGGTCT TCAGCGTATG CTACAGCTCT TGAAGCACCT TGGCAATCCA 
    CAGGAATCGT TTTGTGCTGT ACAAATTGCT GGGACTAATG GTAAAGGTAG CATTTGTAGC 
    TATATATACA CGAGTCTTTT GCAAGCTGCT ATTAAAACAG GACGCTATAC AAGTCCTCAT 
    TTCTTAGAGC CAAGAGATAC CATAAGCATA AATGGTCAGA TTGCTAGTGA AGAGATATTT 
    AATACTTGTT GGAAGCAGGT GATTGAAGTG GATAGAAGAT TTCGTACAAA AGCGACGGAA 
    TTTGAATTGT TAACTGCTAC TGCGTTCCAA TGTTTTCATC ATTCAGGTGT TCGTGTGGCT 
    GTAATTGAAA CAGGAATGGG TGGTCGTTTG GATGCTACTA ATGTATTTGA AGAACCAGTT 
    CTTTCAATAA TTTCACGTAT TTGCTTGGAC CATCAAGCAT TTTTAGGAAA TACCTTAGAG 
    GCTATTGCTA AAGAAAAGGC TGGGATCTTT AAAAAGAATG TTCCGTGCGT GGTTGATGGG 
    TTAAATGAAG TTAACGTCCT AAATCAATTA AAATTAAGTG CAGAGGAAAC GAGGGCACAT 
    CCGTTTTATT TGGCAAAAGG AAAATCAGGT GAAAACAAAA ATGAGTGGAT CATCAATACT 
    CCAAATTGGG GAACCAATAC TTTTAGTACT CCATTGAAGG GGGATTATCA AGGACAAAAT 
    TTGGCTTGTG CAGTAACTGC TCTCGATATC CTTTCTTCGT CATTTTCAAT TATGCTTCCC 
    CATGTACAAA ATGGTGTAAA AAATACATCT TGGCCTGGAA GGCTTGACAT TCGTTCGGTT 
    CCTTCTTTGG GTGATATTTT ATTTGACGGA GCACACAATA AAGAAGCTGC GATTGAGCTT 
    GCGAAATTTG TAAATTCGCA AAGACGTGAG CACAATAAAT CAGTATCATG GGTAGTAGCT 
    TTCACTAACA CAAAAGACGT TACTGGGATT ATGAAAATCC TTCTTCGTAA AGGAGATACC 
    GTTATAGCGA CAAATTTTTC TTCAGTAAGT GGGATGCCCT GGATTAAATC AATGGAACCT 
    GAAGTTATAA AAAACAGTAT ATCTTCGGAA TCTTCTGTGG AATGCTATAC TGCAGATAAT 
    CTAACAATAA GCGAAATTTT AAGACTGGCA AAAGAGAAGA ATTCCAGCGT AATTGTATGC 
    GGTAGTCTTT ATCTTCTAGG AGACATGTAT CGTTATTTAA AACTGGACGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_592963.1
    CDS458..1711
    Translation

    Target ORF information:

    RefSeq Version NM_001018363.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- folylpolyglutamate synthase (predicted) (SPAC227.09), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018363.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCCTATTC AGCTAGGTCT TCAGCGTATG CTACAGCTCT TGAAGCACCT TGGCAATCCA 
    CAGGAATCGT TTTGTGCTGT ACAAATTGCT GGGACTAATG GTAAAGGTAG CATTTGTAGC 
    TATATATACA CGAGTCTTTT GCAAGCTGCT ATTAAAACAG GACGCTATAC AAGTCCTCAT 
    TTCTTAGAGC CAAGAGATAC CATAAGCATA AATGGTCAGA TTGCTAGTGA AGAGATATTT 
    AATACTTGTT GGAAGCAGGT GATTGAAGTG GATAGAAGAT TTCGTACAAA AGCGACGGAA 
    TTTGAATTGT TAACTGCTAC TGCGTTCCAA TGTTTTCATC ATTCAGGTGT TCGTGTGGCT 
    GTAATTGAAA CAGGAATGGG TGGTCGTTTG GATGCTACTA ATGTATTTGA AGAACCAGTT 
    CTTTCAATAA TTTCACGTAT TTGCTTGGAC CATCAAGCAT TTTTAGGAAA TACCTTAGAG 
    GCTATTGCTA AAGAAAAGGC TGGGATCTTT AAAAAGAATG TTCCGTGCGT GGTTGATGGG 
    TTAAATGAAG TTAACGTCCT AAATCAATTA AAATTAAGTG CAGAGGAAAC GAGGGCACAT 
    CCGTTTTATT TGGCAAAAGG AAAATCAGGT GAAAACAAAA ATGAGTGGAT CATCAATACT 
    CCAAATTGGG GAACCAATAC TTTTAGTACT CCATTGAAGG GGGATTATCA AGGACAAAAT 
    TTGGCTTGTG CAGTAACTGC TCTCGATATC CTTTCTTCGT CATTTTCAAT TATGCTTCCC 
    CATGTACAAA ATGGTGTAAA AAATACATCT TGGCCTGGAA GGCTTGACAT TCGTTCGGTT 
    CCTTCTTTGG GTGATATTTT ATTTGACGGA GCACACAATA AAGAAGCTGC GATTGAGCTT 
    GCGAAATTTG TAAATTCGCA AAGACGTGAG CACAATAAAT CAGTATCATG GGTAGTAGCT 
    TTCACTAACA CAAAAGACGT TACTGGGATT ATGAAAATCC TTCTTCGTAA AGGAGATACC 
    GTTATAGCGA CAAATTTTTC TTCAGTAAGT GGGATGCCCT GGATTAAATC AATGGAACCT 
    GAAGTTATAA AAAACAGTAT ATCTTCGGAA TCTTCTGTGG AATGCTATAC TGCAGATAAT 
    CTAACAATAA GCGAAATTTT AAGACTGGCA AAAGAGAAGA ATTCCAGCGT AATTGTATGC 
    GGTAGTCTTT ATCTTCTAGG AGACATGTAT CGTTATTTAA AACTGGACGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPAC227.09 ( NM_001355981.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342926.1 Schizosaccharomyces pombe putative folylpolyglutamate synthase (SPAC227.09), mRNA.
    Price & Availability↑
    CloneID OSc103236
    Clone ID Related Accession (Same CDS sequence) NM_001355981.1
    Accession Version NM_001355981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative folylpolyglutamate synthase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCCTATTC AGCTAGGTCT TCAGCGTATG CTACAGCTCT TGAAGCACCT TGGCAATCCA 
    CAGGAATCGT TTTGTGCTGT ACAAATTGCT GGGACTAATG GTAAAGGTAG CATTTGTAGC 
    TATATATACA CGAGTCTTTT GCAAGCTGCT ATTAAAACAG GACGCTATAC AAGTCCTCAT 
    TTCTTAGAGC CAAGAGATAC CATAAGCATA AATGGTCAGA TTGCTAGTGA AGAGATATTT 
    AATACTTGTT GGAAGCAGGT GATTGAAGTG GATAGAAGAT TTCGTACAAA AGCGACGGAA 
    TTTGAATTGT TAACTGCTAC TGCGTTCCAA TGTTTTCATC ATTCAGGTGT TCGTGTGGCT 
    GTAATTGAAA CAGGAATGGG TGGTCGTTTG GATGCTACTA ATGTATTTGA AGAACCAGTT 
    CTTTCAATAA TTTCACGTAT TTGCTTGGAC CATCAAGCAT TTTTAGGAAA TACCTTAGAG 
    GCTATTGCTA AAGAAAAGGC TGGGATCTTT AAAAAGAATG TTCCGTGCGT GGTTGATGGG 
    TTAAATGAAG TTAACGTCCT AAATCAATTA AAATTAAGTG CAGAGGAAAC GAGGGCACAT 
    CCGTTTTATT TGGCAAAAGG AAAATCAGGT GAAAACAAAA ATGAGTGGAT CATCAATACT 
    CCAAATTGGG GAACCAATAC TTTTAGTACT CCATTGAAGG GGGATTATCA AGGACAAAAT 
    TTGGCTTGTG CAGTAACTGC TCTCGATATC CTTTCTTCGT CATTTTCAAT TATGCTTCCC 
    CATGTACAAA ATGGTGTAAA AAATACATCT TGGCCTGGAA GGCTTGACAT TCGTTCGGTT 
    CCTTCTTTGG GTGATATTTT ATTTGACGGA GCACACAATA AAGAAGCTGC GATTGAGCTT 
    GCGAAATTTG TAAATTCGCA AAGACGTGAG CACAATAAAT CAGTATCATG GGTAGTAGCT 
    TTCACTAACA CAAAAGACGT TACTGGGATT ATGAAAATCC TTCTTCGTAA AGGAGATACC 
    GTTATAGCGA CAAATTTTTC TTCAGTAAGT GGGATGCCCT GGATTAAATC AATGGAACCT 
    GAAGTTATAA AAAACAGTAT ATCTTCGGAA TCTTCTGTGG AATGCTATAC TGCAGATAAT 
    CTAACAATAA GCGAAATTTT AAGACTGGCA AAAGAGAAGA ATTCCAGCGT AATTGTATGC 
    GGTAGTCTTT ATCTTCTAGG AGACATGTAT CGTTATTTAA AACTGGACGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342926.1
    CDS305..1558
    Translation

    Target ORF information:

    RefSeq Version NM_001355981.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative folylpolyglutamate synthase (SPAC227.09), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001355981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCCTATTC AGCTAGGTCT TCAGCGTATG CTACAGCTCT TGAAGCACCT TGGCAATCCA 
    CAGGAATCGT TTTGTGCTGT ACAAATTGCT GGGACTAATG GTAAAGGTAG CATTTGTAGC 
    TATATATACA CGAGTCTTTT GCAAGCTGCT ATTAAAACAG GACGCTATAC AAGTCCTCAT 
    TTCTTAGAGC CAAGAGATAC CATAAGCATA AATGGTCAGA TTGCTAGTGA AGAGATATTT 
    AATACTTGTT GGAAGCAGGT GATTGAAGTG GATAGAAGAT TTCGTACAAA AGCGACGGAA 
    TTTGAATTGT TAACTGCTAC TGCGTTCCAA TGTTTTCATC ATTCAGGTGT TCGTGTGGCT 
    GTAATTGAAA CAGGAATGGG TGGTCGTTTG GATGCTACTA ATGTATTTGA AGAACCAGTT 
    CTTTCAATAA TTTCACGTAT TTGCTTGGAC CATCAAGCAT TTTTAGGAAA TACCTTAGAG 
    GCTATTGCTA AAGAAAAGGC TGGGATCTTT AAAAAGAATG TTCCGTGCGT GGTTGATGGG 
    TTAAATGAAG TTAACGTCCT AAATCAATTA AAATTAAGTG CAGAGGAAAC GAGGGCACAT 
    CCGTTTTATT TGGCAAAAGG AAAATCAGGT GAAAACAAAA ATGAGTGGAT CATCAATACT 
    CCAAATTGGG GAACCAATAC TTTTAGTACT CCATTGAAGG GGGATTATCA AGGACAAAAT 
    TTGGCTTGTG CAGTAACTGC TCTCGATATC CTTTCTTCGT CATTTTCAAT TATGCTTCCC 
    CATGTACAAA ATGGTGTAAA AAATACATCT TGGCCTGGAA GGCTTGACAT TCGTTCGGTT 
    CCTTCTTTGG GTGATATTTT ATTTGACGGA GCACACAATA AAGAAGCTGC GATTGAGCTT 
    GCGAAATTTG TAAATTCGCA AAGACGTGAG CACAATAAAT CAGTATCATG GGTAGTAGCT 
    TTCACTAACA CAAAAGACGT TACTGGGATT ATGAAAATCC TTCTTCGTAA AGGAGATACC 
    GTTATAGCGA CAAATTTTTC TTCAGTAAGT GGGATGCCCT GGATTAAATC AATGGAACCT 
    GAAGTTATAA AAAACAGTAT ATCTTCGGAA TCTTCTGTGG AATGCTATAC TGCAGATAAT 
    CTAACAATAA GCGAAATTTT AAGACTGGCA AAAGAGAAGA ATTCCAGCGT AATTGTATGC 
    GGTAGTCTTT ATCTTCTAGG AGACATGTAT CGTTATTTAA AACTGGACGT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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