tra1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tra1
Entrez Gene ID	2541265
Full Name	SAGA complex phosphatidylinositol pseudokinase Tra1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021677.2	NP_595777.1	SAGA complex phosphatidylinositol pseudokinase Tra1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	trrap	XP_001919276.4
Drosophila melanogaster (fruit fly)	Nipped-A	NP_001097192.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005533	XP_556172.2
Kluyveromyces lactis	KLLA0F22066g	XP_456065.1
Neurospora crassa	NCU01379	XP_960936.2
Arabidopsis thaliana (thale cress)	AT4G36080	NP_680770.2
	TRRAP	XP_001093586.2
Bos taurus (cattle)	TRRAP	NP_001179066.1
Mus musculus (house mouse)	Trrap	NP_001074831.1
Xenopus tropicalis (tropical clawed frog)	trrap	XP_004918032.1
Homo sapiens (human)	TRRAP	NP_003487.1
Canis lupus familiaris (dog)	TRRAP	XP_005621163.1
Saccharomyces cerevisiae (baker's yeast)	TRA1	NP_011967.1
Magnaporthe oryzae (rice blast fungus)	MGG_09523	XP_003712218.1
Eremothecium gossypii	AGOS_AER393C	NP_985248.2
Schizosaccharomyces pombe (fission yeast)	tra1	NP_595777.1
Arabidopsis thaliana (thale cress)	AT2G17930	NP_179383.3
Pan troglodytes (chimpanzee)	TRRAP	XP_001136582.1
Rattus norvegicus (Norway rat)	Trrap	NP_001099377.2
Gallus gallus (chicken)	TRRAP	XP_004945166.1
Caenorhabditis elegans (roundworm)	trr-1	NP_001022032.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The changed division response (Cdr) kinases Cdr1 and Cdr2 are negative regulators of Wee1 and they are required for the Tra1-dependent alterations to Wee1 function.
Title: Transformation/transcription domain-associated protein (TRRAP)-mediated regulation of Wee1.

The following tra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100223	NM_001021677.2 Latest version!	Schizosaccharomyces pombe SAGA complex phosphatidylinositol pseudokinase Tra1 (tra1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tra1 ( NM_001021677.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595777.1 Schizosaccharomyces pombe SAGA complex phosphatidylinositol pseudokinase Tra1 (tra1), partial mRNA.

Price & Availability↑

CloneID	OSc100223
Clone ID Related Accession (Same CDS sequence)	NM_001021677.2
Accession Version	NM_001021677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	SAGA complex phosphatidylinositol pseudokinase Tra1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021677.1. COMPLETENESS: incomplete on the 5' end.

ATGCAGCTTG CTCTATCCCA CAACATTCCA ATAGTAATTG TTAAATACTT AGGAAAACGT 
ATCCGAGTGC AATTGGAATT TAAAAAAGAG CTTGCGCAGA GATCCAAGCA TCTGATGGAT 
GTATCACAAT GCGAACATTG GCATAATCGG CTTTGTGATG TTGGATTAGA CTCGAAGCAA 
AAGGCAAACA CAGCAATTGA AATTCGAGAT GCACTGGATG ATGTGTTGGT AACTGAAAAG 
ACGAATTTTG AAACTTTCAT TCCTCTTTTG GAGGACACGT TGTCTTTGCT TGAAAAGGAA 
CGTCCTGTAT TTTCATCGTT AGCAGCTACA CATCGTTTAC GCATTGCATT GCTTGAATTG 
CTCAAGAAAT CTGGATCCTA TAAAGGTTTT GAAGCATTCG TTAATCGGAC TTTTGCCGTG 
CTTCTTCGTA TAGTCGTTAA TGATAATGAA GAGATGGCAG TGTTGGCATT GAAGCTTGTT 
GTCCTTCTTT TTAAGGACCA CAGTAGCCTT GCTAAGGGAC ATGTTCAGGA ATTTTTATCC 
ATTGTCGTTG AAAACTACAA ATCCATGACC ACAGTTGTTT CAGAAGCTTT CCCACCTAGA 
TCAGCTCCGA ATACTCCATC CTCTCATCCC ATGTCTGCTG CATCTTCTGC ATCTCCTGCT 
GAAATTGGAA TGGAACATGC AGGTCCAAAA ATGATCCCAA AAGCTTCTTC GAGTTTCAAG 
GTCACTGCTG AATTTCCCAT TATCGTCTTT CTCCTATTTC AAACATACAA AGATCTTATT 
CCGAAAATGC TTCCTTTACT TGCTCCGCTT GTACTTCAAT TTATCTCACT TCGTCCTCCA 
CCTCAAGCCG AAGCTCGTCG ACTTGCAGAA TCTCAAAAAG AGGTTTTTAT TGGTGTAGTC 
CCTTCTTTGC GTCGAAATCA TCTTTACAAT GACCTTATTT CTGCGCAAAT TAAATCATTC 
TCTTTTCTCG CCTATTTATT GAGAAGTTTT GGTGCTGCTC TTAAACAGTT TGAAAGCTCG 
ATCCCAATAT GTACCTTGCA ATTGTTCATG GACTGTCCTT CCGAATTATA TCAGACTCGA 
AGAGAACTTT TGGTAGCTAC AAGGCACGTC TTATCGACGG ATTATTTGAG AGGGTTTCTA 
CCGTATGTAG ATCAACTACT TGATACCAAA ATTCTAGTTG GTTCTGGAAT CACTTCTCAG 
CATTCATTAC GACCCATGGC TTTTAGCATG CTGGCAGATA TGCTTCATTA TGTGCGTATG 
GAATTAAGTC CACAACAAAT TTACAAAGTC ATACTTCTTT ATTTCAGTAT TTTGATGGAC 
GACTTTTATA CTTCAGCTAT TCAGGCAATG GCTACAAAGC TTATTTTAAA CTTGGTTGAA 
AGAATTGTTG CACTAGAAGA TTTTTCCACT AGCCGATCAC TTCTATTTGC TATACTTTTA 
TGTCTCTTAA GAAAATTGAC TTCTCTGAAT TTCGAGTTTA TGAAGCTGAG AGATTCTTTA 
CAGGAAAATG CTGACTTAAA GCAAATTAAA ATAGAAGAAA ATAAACATGA TCTTCCTATG 
TTTGAGAACC CTACTGGAGC TGCCCAACCT TCAGGTTTGG ATAAGCTAAA AGATTGCATA 
TTTCTTTTTA AAAACACCTT ATTAGGTATT AAGCCTGTTT TGTTTGGACT AAAGCAACGA 
AATATTCCTC TTGCCAACGG TTCCATTTTT ACAGCTCAGG AATGGTCTGA AAAGTTACAT 
CTTAGTTCAA CTAATGAAGT TTTACTGTTT CGAAGATTAC TTGTGGAAAG TTTAAAGGGA 
TTCAGTTATT ACCAGACAGA TGAAAAAACC GGTGTATTCA AGTCCTCGAA AAATCTTGCA 
TACTCTCAAC TTGATTCTTC GTTGACCACA AACCCTTCTA AACTCTTAGA AGAGAAGGAG 
CTCTTAGAAA TGTTAGCAAC TTTATTTTTA CACTTGGACC CATCTGTTTT CGTCGAAATT 
TTGGAATCCG AGTTTCCGAA TATATTTGAG TGTCTAGTAG ATAATCTTGC TTTATTGCAT 
ATTTTCCAAT TTTGGATGTC TAATGAAGTT ACTTCTGTCA ACTGCACGGG CATCGTGCTG 
TCTTTTTGCT GTGACAATCT AGCTAAAATA GGGTCTGGCC AATCCACTCG GGTTTCTGTT 
TTATTGCGAT TATTCAAGTT GGCCTTTATG ACTGTGAATG TTTTTCCCGA AAAAAATGCA 
GAAGTCCTGC GTCCCCATAT ATCCTATATC ATTTCAACTA GCCTCGAATT GACTACTGAT 
GCTGTTGAAC CTTTAAATTA CGTTTATTTA ATGAAAGCTT TGTTTAGAAA TATCAGTGGA 
GGAAAATTTG ATTCTCTTTA CAAGGAAATA CTACCACTTC TCCAAGTAAT GCTAGAATGT 
TTCAACAGAT TGATTTTTAC TGTTACGTCT ACATCCCAAA AAGAGCTGTA TGCGGAACTT 
TGTTTGACTT TACCCATTAG ACTCAGTGTG TTGTTGCCTC ATATGAATTT TTTAATGAAG 
CCATTGATTG TTGCATTAAA AGGCCCCCCT GAAATAGCTA GTCAAGGGCT TCGAATATTT 
GAACTTTGCT TGGATAATCT TACTCAAGAA TTTTTGGATC CTTTATTGGA CTCAATTATG 
CCGGATTTGC TGATATGCCT GTGGAATCAT TCACGCCTTA ATCAATCCAA TAATCAATTG 
CATCAAAGTG CTGTGAGAAT ATTAGGAAAA ATGGGTGGTC GAAATAGACA AATATATCTT 
GGTACCTTTG GCTTTGATTT TTTGCAAGAT GAAAATATTT TTCCTTCAAT CCAGTTTTCT 
TTCCAAGGCT CCTCTCAGAA TTTTAGCTTA GAGCACTCCA AATTTTTAAT GTCTTCATGT 
GCTGTTTTAA ACAATCAAAA TTCCGATTTA GAAGAAAAGA AGCAAGCATT TCAAATGGTC 
AAGAATTCGT ATTTATTACT CTTTGCGTCT GCGAAGCCCG ATGAAGACTT TTGGGAATCT 
ATTGATACAA TGTGCCGAGC AGTTGTAGAT CGCATGGATA AAAACTTACA GCAAGTCTCT 
AATGGTAGGT TGTGCCCTGA CAAAGATGAA TCTTATTATC TGCAAAGATC TATTGTCTCA 
AACATTTTTA AATCTTTAGT CGGCTCAATG TCTTGTGTGG AATTTGTAGC TGAAGCTCGA 
GAAACCATCA ATCGTTCTTT GGAATGGCTC ATTGTTCTTG ATTTGGTTAA TTACGCTGAC 
TCTCTGCAAA TAAAAGATCA AAATATTTTT GATAATTTGC AAAGCATTAA AATGCTTGAT 
CTTACTACGT GCATCAACGG AATTTTCGAA TCTTTATGTT CTGAAAATGA AAATACCAGG 
TCTAATGCTT TATCGTGTAT CGATCATTAT TTGAACGCCC ATAAGATGCT TCTCAACACA 
ACATTGGATA TATCAAAGTT GCCTAGTTTT CAAAATCTTG TAACAGTGTT TTGCCAAAGT 
TGCCATAAGG AACTGTGGTA CCAAAAGAAT GCTGGATTTC TCGGCTTAAA AGCAATACTT 
TCGTATGATT CACATCATAA GCTTTGGATC CAAGATAGAT TACACGACAT ATTGAAGGCT 
TTGTTCTTTA TTCTCAAGGA TACTCCAACC GATTATGGGG TTTTAAAATT AACCGAAGTT 
CGTTCTTTCA TCGTTGATAT TACAACTCAA TTCTGTATAC TTCAGGATGT GCTTGCACCG 
AAAGAGCGTG CTAATAATAT AATTAATGCA TTTTCTCCCT TTTTTTTAGA ACTCCTACAT 
CCCAACGATC ATGTTAGGAA TACTGTGCAA CAAGCTATTG AGAACATTTC CAACAATTCT 
AAACTTAGTG TGGTTGATCT TCTTCTTCCT ATAAAGGACA GGCTTTTAAG CCCTATTTTT 
GGAAAACCCT TAAGAGCGTT ACCTTTTACA ATCCAAATAG GGCATATAGA TGCAATCACT 
TACTGCTTGC ACCGCTCTCC CTCTTTTTTG GATTTGACTG ATGAATTGTA CAGATTATTT 
CGTGAAACAA TAGCATTAGC TGATGCTGAA GATGAAGCAT TAGTCACCAT GCTTAAAACT 
TCTCAAAGCA AAGATAGCTC TTCTTTACGA AAATTAAGGG CTACTTGCTT GCATTTACTA 
TTTGCTTCTT TGGTGGCTCA TAAGTTTGAT CAACCTCAAC ATGCTCAAAC TCGTACTAAA 
ATCATAGCGA TATTTTTCAA AGATCTATAC TCACCTCACA AAGAAATTTA TTCAGTTGCA 
ATAGATGCGT TAAGGCACGT GCTCTCGCAA AACCAAAAGC TTCCTAAAGA ACTTCTTCAA 
AGCGGCTTGA GACCAATTTT AATGAATTTA TCGGACCACA ATAAGCTGTC AGTTAACGGT 
CTTGAGGGTT TATCCAGACT TTTAAGATTA CTAACTAATT ACTTTAAAGT GGAAATAGGT 
CGGAAGCTTC TTCAACACCT TAATGTTCTA TCTGATTCTA AAGTCCTAGA GACTGCCTCA 
CTTTCGTTAT TGAAAACCAA CCCAAGGATC GAGATTATTG TGTCTTTGGT TAACGTATTC 
AGAGATCTTC CTCCGTTGTC AGCGCAGTTT CTAGGTGACT TGTTGTCGTC GGTCGTAAAC 
ATAGAGGCAG TTCTCAGAAA ATATTCTAAC AGCCCATTAA GAAAACCGTT GTATTCTTTT 
ATGGATTTGC ATGCAAATGA TACCTGGATG TATATTTTGA ATAATGCACG TAATGGCGAT 
TTAATAACTC GATTCGTTGG TGCTTTGAAT GACCCGATGT CTGAAAAACT TCGCGAAACC 
GCCGGTAATT ACTGGGGAAA GCTTTTGGAG CTAATTTCGC AACCGGTGTC TGTTGAAAAT 
TTGGCTCCAA TGTATGCTGT TGATATTATT GCAACCGTGT TTCCTTACAT TTCTGCCAAT 
GTAGATGCAG GAGTTATATC GGCTAAATTT ATTGTTTTAT CAAAGTCCTT GTATGGAATG 
CTTAGTTCAT ACAACGAATA TCTATTTCTT CCTATTCGCA GGTGTATATC GTCCATTACA 
AAAATGCTTT TATCTAGTTT AAAGAAAATA GAGAAGAAGC TCGAGTTTTC ATTAGAAGTT 
TTTAGATTTA AAGCTGACGA TGATAACGAT TTCCTTCCAG AATACATAGA TTCGTTATGT 
GGTTGTCTTA TAACCTCAAC AAGTGCAGCT GAAAAGAAAA GTATATTTTT AGTTTGCTGC 
AGTATAGTTG GTGACAAGTC AGTTCGACCT TTTTTTAAAG CATTTCTCTT GGATAAAGTA 
ATCAATCCGC TTGTATTTAA GTCATGCGGA GAAAATAACT TTATTGATAA GGATGTTGTT 
CATTCTGTTT ACACTCATGT TTGGCGTGTT TCAATTCGAG ATTTTGCTGA AGTATCGGGA 
GCCACAGATT CCTTTTACAT GGGAATTATG TGCTTGACAA CGGCGCTTTG TAAGTACCAT 
TCAGCACTTC TTAATGATTA TCGCAAGAGC GTTATAATGT CAGCTTGGAA CTACATTAAA 
CTCGAAGATC CTATGGTAAA ACAAGCCGCT TACGCTACGA TTGCATGTTT TATAAGTGCG 
TATGATACAC CTGCTAAGAT TGTGACACCT GTTTATGTTT CTATACTAAA AACATATCAA 
CCAGAAGTGA GAGCTTTTAT TGAATTCTCT CTTGCTTCTT TGCTGTCTGT ATTAACCGCT 
AGGCTATCGA GTCCTTCCGA TTCAACTTTC CCCCTTTGGG CGAAACTTCC TAGATTAGTT 
ATCTCCGAAG ATGTTCAGGG TATATCACAG CCTTTGACAG TTTACCAATT CATATGTAAA 
GCACCTGACC TTTTCTTCTC TTGTTGCTCT CATTTTATTG TACCCATGGT TAATGCGCTT 
CCAAAGCTTG TGTCGTTTTC TAGTGCTAGT ACAGAACCAC GAAAGTTAGC TTTGGATATT 
GTTCAAACCT TTATAAATTG GCAGAGGAAA CAGAATGAAT CAGAAAATTC CGAGACCACG 
CTTTTTAGCA ATTCCCATAT TGAAGCTATT CTTACCTTCC TTATAAAATT CCTTTCGCTT 
TTTCCCGAAC CAGTTGAAGA AAATCCACTT TCAAAGAAGG GGTTATCATT ATTTAATGAT 
CTGTTTAGTT TTCCTCGATG GAAAGACTGT CAGCTGAACT CTAACTTCTT TGAAAAAATC 
CTTGTTGATA TGGACTTCAA CGATAATAAC TATCGTACGG TAGCTAACAC ATTGTTTGTG 
TTTGGAGTTA TTTTAAAGAA CAGAGGTATG GAGTATATTC AACGTGAATA TAGTCATATA 
ATCGCTCTGA TCGACAAAAG TTTGCGTTGT GGTAAATTGC CTGTTACTCA TTCATTGGAG 
CAGATTATTT TATTACTGTT ACAAAGCCAT CCAACTCAAG CAGAAGAAGA AGAAGACACG 
AATGAGGCTG ATGATTTTAA ACAACTACTA CTTTCAGTTA TTCATGATAA TCTCGCCGCT 
GCCACTAACA TTGAGTCTGC TATTTGCTAT TTGCAAATTG TTAAATCCTC TAATCCGGAA 
GCGCTTGATG GTCTTCTTCT TCCCTTAAAT AAATGCTTTC AAAAGGTAGC ACGAGACCAT 
ATTGTCGCTT GCATGCAATC TGCTATACAA GCATCTGGAA AAGTTACACT TCCTTCTGCA 
TCTGATACTG TCTCAAAGCT TTTGATTAGT TTCATTGAAA TTATTAGAGT ACGCATGGCA 
AGCTTAGGAG ATCAACGTAG ATGGTTTTTA AGTGTTGTGG TGCAATTGCT CGAAAAGAGT 
TCAAGTTTTG AGCTTTGTGA ACATATTCTT AATGTTACAA AGGAATGGGT GATTGTGAAA 
AGAGATTCGT TTTTGACAGT CAAAGAAAAA ACTGCACTTT TATTGAAGAT GAGAACTTTT 
GAGGGAAGAT TTGACAACAA ACTATACATC GAAGCATGTG ATTTGCTATC CACGATATAC 
AGGGATCCCA TTTTTGCGCA TACAGAATTA ACTGCCCGAT TGAAGCAAGC CTTCCTTCTT 
GCTACGGCAT CTAAAGATAC CAAAATACGA ATGGATTTTA TGGATATTTT TGATTCCAGC 
ATGTCTCGTA ATGTTTATTC TAGATTGACT TTCATACTGG ATGCAACTAG CTGGGATACA 
ATTCCTAGCA TTTACTGGAT CAAACAAGCG AATTATATTC TTCTTGGGGC TATAAATGCG 
AAACAGCCTG TCCGGCTTAC CGACAATAGT CTTCGATTTG CTCCAGTGCC AATGACAAAA 
CCCATATTAA GCTCACTTCC TGAAGTTTTT TCAAAACACA ACGGTTCAGC TATTCCTCTT 
GGCAGATTTA CCTTTTTCAA ACAACTGGAT TTGTTCCTAA AAAGAAATAA GGAATTAACT 
GTGCAAAAAA TTATTTTTCC GTTGGCACAC ATTCAAATGC TCAGCGATGC AGATGCTAAT 
AAGCTTTGGC AATATATTTT TCCTCTTGCA TGGAAAATAC TAAGTTCGGA TAACCGTTCT 
GACCTTTCAA AGTCTTTAAT CTATTTACTC ACTCGTGATT ATCACATCAA ACAGGTAAAC 
AATCGACCGA ATGTGATCTC TACTTTGGTT TCATCTTTCG TTAAGTGTGC TGCAAAATTA 
GAGCTTCCAC CTCATTTGGT AAAATATCTT GGAAAATTGT ACGGTGTATA TCATGAATCC 
GTTTCACTTC TCGAAATTCA GCTGAGTGAT AAATATGACA TGTATCAGAA TGCAAAAGTT 
CAAGAAAGCC GTGCGGATGC AGTCGCAGAG TTATATGCAT CTTTAAATGA AGATGATATG 
TTTTATGGCC ATTGGCGACG AAACTGTAAG TACTTGCAAA CTCAAGTTGC TCTCTCGTAT 
GAGCAACTTG GAATGTGGGG AAGGGCTCAA CAACTTTATG AACAGGCACA GACCAAGGCT 
CGCAGTGAAG CCATTCCCTT CTCAGAATCA GAATACAACT TGTGGGAGGA TCAGTGGGTA 
ATGTGCGCTC AAAAATTACA ACAATGGGAC GTCTTGACAG AGTTGGCGAA GCACGAGGGA 
TCTTCTGAAC TATTGCTCGA GTGTGCTTGG AGAATTAGCG ATTGGTCTAA TAATCGTGAA 
TCCTTAGAGG TTGCCATTAA GAGCCTTTCT GATGTTCCTA CTCCACGGAA ACTTACCTTT 
CAATGCTTTA TGACACTTCA AAAGTCAGTA TCTCAACCTC TTGCCATTAA AGAGTTCCAA 
CAAGTATTGT CTGAAGCCAT TCAACTTGCG TTAATTAAGT GGCATCAACT TCCAGAAAAA 
GTTAATCAAT CTCATTATTC ACTTTTGCAT CTGTTTCAGC AATTTGTTGA ACTACAAGAA 
GCTGCTACAA TTTATTCACA TCTGAATGCT ATCAATTTTC AGAATTTGCC TACTAATGTT 
CAGTTGATTA AAAGTGCTCT TCAAGTTTGG CAAGAGCGGT TACCTAATGT CTGGGACGAT 
ATTAATCTTT GGCGTGATCT CATTTCTTGG AGACAAATAG TATTCTCTAT GATAAATCGT 
GTTTATCTCC CCTTGGTCCC TACGATACAG GCTAATAGTA GTGCTGATTC GTCTAATCCT 
CCGAATACCT CTTTCTTATT TAGAGGATAT CATGAAACCG CTTGGCTAAT TAACCGATTC 
GCTCATGTTG CCAGAAAACA TAAACTACCT TCCGTATGTC TGAACCAATT AACGAAGATA 
TATACTCTTC CAAATATTGA AATTCAAGAA GCTTTTTATA AGCTTCGAGA GCAAGTTTTA 
TGTTATCTAC AAAACCCAAG AGATCTGAAA ACAGGTTTGG AGGTTGTTAC AAATACCAAT 
CTGATGTACT TCAATTCTAG ACAAAAGTCA GAATTCGTGA CATTAAAAGG AAAATTTTTA 
GAAAAACTAA ATCGAGGTGA GGAGGCTAAC CAAATGTATG CTGCAGCAGT ACAAATTGAT 
TTAGGGTTGC CAAAGGCATG GGCAGAATGG GGTCGTTACA ACGATTTGCT TTTCAATAAG 
TCACCAGATA ATTTGAGTGC AGCATGCAAT GCCATTAGTT GCTATCTTCA AGCTGCTGGT 
ACTTACCAAA GTTCTAAAGC TCGTAAGATG CTGGCAAGGG TATTGTGGTT ATTAAGTTTG 
AATGACAGCG CCGGTACTAT AGTTAAAGCT TTTGAATCAT ACAAAGGTGA TATACCAGTT 
TGGCATTGGC TTACCTTTAT TCCCCAGCTT TTAAATTCAC TTTCTAAAGG GGATACTAAA 
TGCGCACCTG TTGTTCTTAA AAAAATTGCT AAATCCTATC CACAGGCACT ATTTTTCACT 
TTAAGAACTG CTCGTGAAGA TATTGCACAG GTAAAACGCA CTGAGATGGC AGCATGGAAA 
TCAAATACGA CTGATGAAAA TAAAAGAAAT GAGGATATTT TAAGCATACA ATCTAACTTT 
CTTTCTACCA ATCAAAGCAC GAATGCTCCA GCAACAAATA AAGAAGATGG ACTTTCTAAA 
AAAGTTTGGG AATATATTGA TGAAATTATG TCAATTTTAA AAACTGCTTA TCCTCTATTG 
GCTTTGACTA TGGAAACTTT AGTTGATCAA ATTCAAGCGA AGTTTAAATG TAAGAATGAT 
GGAGATGCTT TCAGGCTAGT GGTAGCCTTG CTTAATGATG CTGTACAGCA TAGCATTCGT 
TTGGGGATCG TAACGGATGA AATGAAACTT CCTTCTTCAA CCGAATCGAA CTTAAGTTTG 
TTTGCTGATA ATATTCTCCC TGACTATTGT AAACAACTAT TTAAGGAAGA TTTCATTGTC 
AATTCAAACG GTTTGAAATC ATATATTTTC AAACTAAGGA AATGGAGATC ATATTTTGAG 
CGGTTATTAT CTAAGGTTCC TAAAAAACAA TATTTGGAAC AATACAGCTC ATTCTTGTGT 
GAATTTCATC ATCAAAAATT TGATGAAATA GAAGTTCCTG GACAATATCT TTTGCACAAG 
GATAACAACA ATAGTTTTTC TTGCATAGAA AGGTTTCTTC CTGAAGTGGA ATTGATAGTA 
GGACATGGCG TTTGCTATAG AAGGTTATCC ATTCGAAGCA ATGGCGGAAC TATACACCCA 
TTTGTCATTC AGTATCCTTC AGCACGAAAC AGCCGTAGGG AAGAGCGTTT TATGCAGCTG 
ACCAGATACT TAAATGATGC ACTTGCTTTA AATTGTGAGA CTCGAAGACG TTGTTTGAAA 
TTTTACATTC CTGCAGTGAT TCCGTTGTCA TCGCACATCC GGCTGTTAGA AGACCAACCG 
TCTTCTATTA CTCTCCAAAA AATATACGAA ATTTATTCAG AAAGAAATAA CTTCTCCAGA 
GATGATCCGA AAGAGTTGTT TACAAACGAG TTATCAAAAC ATATGATGGA ATTAAACTCC 
CAAATTAGTC AGGAATCTAC TGATGCTGAA AAATTAGCTA ATCGTAAACA ATTGTTTAGT 
AGGAGAATCG GAATGTTTGA AAATATTCAA AAGCTTTATT CTCCGTCAAC GATTTTAAAA 
GATTATTTCT CAAGTATCTT TACTAATTAT TCCGATCTGT GGTTGTTCAG AAAAAATTTT 
TCATACCAAT ATGCTTGTTT TTCATTTATT ACATACATAC TAAGTATAAA TAATAGGATA 
CCTGCTAAGC TTGTTTTTTC TAGAGACTCT GGCGGTGTTT GGACAACAGA AGCGCTGCCA 
TCTATGGTTT CTTCTACTCC AGTTTACCAT AATGGAGAAA TTGTTCCTTT TCGCTTTACC 
CCCAACATTA AAGAATTTAT TGGAAAAACT TGTACTGAAG GTTTACTGGG CCCTTCAATA 
ATGGCTATAG CTCGCGCGCT CTCCAAACCG GATTTCGATT TGGATATGTA CTTGGGTATC 
TTTATTAGAG ATGATCTTTT TTGGTGGCTT GCACAGCAAA CTAAAGGCGT GCCGGCAGAT 
TTCAGTATGC TCAACAAAGT CAATAGCAAT ACTGATCTTA TAATGCGAAG GGTTGCATCA 
TTAAGTCAGG TTGCTTATGG TAACTTACCA GTCAATCAAA CAGCTATTGA TTATTTGGCG 
CAAGCCTCTA GTTCCAAAGT TTTGGCACAG ATGGACGTCT TATGGGCTCC TTGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595777.1
CDS	1..11100
Translation	MQLALSHNIPIVIVKYLGKRIRVQLEFKKELAQRSKHLMDVSQCEHWHNRLCDVGLDSKQKANTAIEIRDALDDVLVTEKTNFETFIPLLEDTLSLLEKERPVFSSLAATHRLRIALLELLKKSGSYKGFEAFVNRTFAVLLRIVVNDNEEMAVLALKLVVLLFKDHSSLAKGHVQEFLSIVVENYKSMTTVVSEAFPPRSAPNTPSSHPMSAASSASPAEIGMEHAGPKMIPKASSSFKVTAEFPIIVFLLFQTYKDLIPKMLPLLAPLVLQFISLRPPPQAEARRLAESQKEVFIGVVPSLRRNHLYNDLISAQIKSFSFLAYLLRSFGAALKQFESSIPICTLQLFMDCPSELYQTRRELLVATRHVLSTDYLRGFLPYVDQLLDTKILVGSGITSQHSLRPMAFSMLADMLHYVRMELSPQQIYKVILLYFSILMDDFYTSAIQAMATKLILNLVERIVALEDFSTSRSLLFAILLCLLRKLTSLNFEFMKLRDSLQENADLKQIKIEENKHDLPMFENPTGAAQPSGLDKLKDCIFLFKNTLLGIKPVLFGLKQRNIPLANGSIFTAQEWSEKLHLSSTNEVLLFRRLLVESLKGFSYYQTDEKTGVFKSSKNLAYSQLDSSLTTNPSKLLEEKELLEMLATLFLHLDPSVFVEILESEFPNIFECLVDNLALLHIFQFWMSNEVTSVNCTGIVLSFCCDNLAKIGSGQSTRVSVLLRLFKLAFMTVNVFPEKNAEVLRPHISYIISTSLELTTDAVEPLNYVYLMKALFRNISGGKFDSLYKEILPLLQVMLECFNRLIFTVTSTSQKELYAELCLTLPIRLSVLLPHMNFLMKPLIVALKGPPEIASQGLRIFELCLDNLTQEFLDPLLDSIMPDLLICLWNHSRLNQSNNQLHQSAVRILGKMGGRNRQIYLGTFGFDFLQDENIFPSIQFSFQGSSQNFSLEHSKFLMSSCAVLNNQNSDLEEKKQAFQMVKNSYLLLFASAKPDEDFWESIDTMCRAVVDRMDKNLQQVSNGRLCPDKDESYYLQRSIVSNIFKSLVGSMSCVEFVAEARETINRSLEWLIVLDLVNYADSLQIKDQNIFDNLQSIKMLDLTTCINGIFESLCSENENTRSNALSCIDHYLNAHKMLLNTTLDISKLPSFQNLVTVFCQSCHKELWYQKNAGFLGLKAILSYDSHHKLWIQDRLHDILKALFFILKDTPTDYGVLKLTEVRSFIVDITTQFCILQDVLAPKERANNIINAFSPFFLELLHPNDHVRNTVQQAIENISNNSKLSVVDLLLPIKDRLLSPIFGKPLRALPFTIQIGHIDAITYCLHRSPSFLDLTDELYRLFRETIALADAEDEALVTMLKTSQSKDSSSLRKLRATCLHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQKLPKELLQSGLRPILMNLSDHNKLSVNGLEGLSRLLRLLTNYFKVEIGRKLLQHLNVLSDSKVLETASLSLLKTNPRIEIIVSLVNVFRDLPPLSAQFLGDLLSSVVNIEAVLRKYSNSPLRKPLYSFMDLHANDTWMYILNNARNGDLITRFVGALNDPMSEKLRETAGNYWGKLLELISQPVSVENLAPMYAVDIIATVFPYISANVDAGVISAKFIVLSKSLYGMLSSYNEYLFLPIRRCISSITKMLLSSLKKIEKKLEFSLEVFRFKADDDNDFLPEYIDSLCGCLITSTSAAEKKSIFLVCCSIVGDKSVRPFFKAFLLDKVINPLVFKSCGENNFIDKDVVHSVYTHVWRVSIRDFAEVSGATDSFYMGIMCLTTALCKYHSALLNDYRKSVIMSAWNYIKLEDPMVKQAAYATIACFISAYDTPAKIVTPVYVSILKTYQPEVRAFIEFSLASLLSVLTARLSSPSDSTFPLWAKLPRLVISEDVQGISQPLTVYQFICKAPDLFFSCCSHFIVPMVNALPKLVSFSSASTEPRKLALDIVQTFINWQRKQNESENSETTLFSNSHIEAILTFLIKFLSLFPEPVEENPLSKKGLSLFNDLFSFPRWKDCQLNSNFFEKILVDMDFNDNNYRTVANTLFVFGVILKNRGMEYIQREYSHIIALIDKSLRCGKLPVTHSLEQIILLLLQSHPTQAEEEEDTNEADDFKQLLLSVIHDNLAAATNIESAICYLQIVKSSNPEALDGLLLPLNKCFQKVARDHIVACMQSAIQASGKVTLPSASDTVSKLLISFIEIIRVRMASLGDQRRWFLSVVVQLLEKSSSFELCEHILNVTKEWVIVKRDSFLTVKEKTALLLKMRTFEGRFDNKLYIEACDLLSTIYRDPIFAHTELTARLKQAFLLATASKDTKIRMDFMDIFDSSMSRNVYSRLTFILDATSWDTIPSIYWIKQANYILLGAINAKQPVRLTDNSLRFAPVPMTKPILSSLPEVFSKHNGSAIPLGRFTFFKQLDLFLKRNKELTVQKIIFPLAHIQMLSDADANKLWQYIFPLAWKILSSDNRSDLSKSLIYLLTRDYHIKQVNNRPNVISTLVSSFVKCAAKLELPPHLVKYLGKLYGVYHESVSLLEIQLSDKYDMYQNAKVQESRADAVAELYASLNEDDMFYGHWRRNCKYLQTQVALSYEQLGMWGRAQQLYEQAQTKARSEAIPFSESEYNLWEDQWVMCAQKLQQWDVLTELAKHEGSSELLLECAWRISDWSNNRESLEVAIKSLSDVPTPRKLTFQCFMTLQKSVSQPLAIKEFQQVLSEAIQLALIKWHQLPEKVNQSHYSLLHLFQQFVELQEAATIYSHLNAINFQNLPTNVQLIKSALQVWQERLPNVWDDINLWRDLISWRQIVFSMINRVYLPLVPTIQANSSADSSNPPNTSFLFRGYHETAWLINRFAHVARKHKLPSVCLNQLTKIYTLPNIEIQEAFYKLREQVLCYLQNPRDLKTGLEVVTNTNLMYFNSRQKSEFVTLKGKFLEKLNRGEEANQMYAAAVQIDLGLPKAWAEWGRYNDLLFNKSPDNLSAACNAISCYLQAAGTYQSSKARKMLARVLWLLSLNDSAGTIVKAFESYKGDIPVWHWLTFIPQLLNSLSKGDTKCAPVVLKKIAKSYPQALFFTLRTAREDIAQVKRTEMAAWKSNTTDENKRNEDILSIQSNFLSTNQSTNAPATNKEDGLSKKVWEYIDEIMSILKTAYPLLALTMETLVDQIQAKFKCKNDGDAFRLVVALLNDAVQHSIRLGIVTDEMKLPSSTESNLSLFADNILPDYCKQLFKEDFIVNSNGLKSYIFKLRKWRSYFERLLSKVPKKQYLEQYSSFLCEFHHQKFDEIEVPGQYLLHKDNNNSFSCIERFLPEVELIVGHGVCYRRLSIRSNGGTIHPFVIQYPSARNSRREERFMQLTRYLNDALALNCETRRRCLKFYIPAVIPLSSHIRLLEDQPSSITLQKIYEIYSERNNFSRDDPKELFTNELSKHMMELNSQISQESTDAEKLANRKQLFSRRIGMFENIQKLYSPSTILKDYFSSIFTNYSDLWLFRKNFSYQYACFSFITYILSINNRIPAKLVFSRDSGGVWTTEALPSMVSSTPVYHNGEIVPFRFTPNIKEFIGKTCTEGLLGPSIMAIARALSKPDFDLDMYLGIFIRDDLFWWLAQQTKGVPADFSMLNKVNSNTDLIMRRVASLSQVAYGNLPVNQTAIDYLAQASSSKVLAQMDVLWAPWL

Target ORF information:

RefSeq Version	NM_001021677.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe SAGA complex phosphatidylinositol pseudokinase Tra1 (tra1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021677.2

ORF Insert Sequence:

ATGCAGCTTG CTCTATCCCA CAACATTCCA ATAGTAATTG TTAAATACTT AGGAAAACGT 
ATCCGAGTGC AATTGGAATT TAAAAAAGAG CTTGCGCAGA GATCCAAGCA TCTGATGGAT 
GTATCACAAT GCGAACATTG GCATAATCGG CTTTGTGATG TTGGATTAGA CTCGAAGCAA 
AAGGCAAACA CAGCAATTGA AATTCGAGAT GCACTGGATG ATGTGTTGGT AACTGAAAAG 
ACGAATTTTG AAACTTTCAT TCCTCTTTTG GAGGACACGT TGTCTTTGCT TGAAAAGGAA 
CGTCCTGTAT TTTCATCGTT AGCAGCTACA CATCGTTTAC GCATTGCATT GCTTGAATTG 
CTCAAGAAAT CTGGATCCTA TAAAGGTTTT GAAGCATTCG TTAATCGGAC TTTTGCCGTG 
CTTCTTCGTA TAGTCGTTAA TGATAATGAA GAGATGGCAG TGTTGGCATT GAAGCTTGTT 
GTCCTTCTTT TTAAGGACCA CAGTAGCCTT GCTAAGGGAC ATGTTCAGGA ATTTTTATCC 
ATTGTCGTTG AAAACTACAA ATCCATGACC ACAGTTGTTT CAGAAGCTTT CCCACCTAGA 
TCAGCTCCGA ATACTCCATC CTCTCATCCC ATGTCTGCTG CATCTTCTGC ATCTCCTGCT 
GAAATTGGAA TGGAACATGC AGGTCCAAAA ATGATCCCAA AAGCTTCTTC GAGTTTCAAG 
GTCACTGCTG AATTTCCCAT TATCGTCTTT CTCCTATTTC AAACATACAA AGATCTTATT 
CCGAAAATGC TTCCTTTACT TGCTCCGCTT GTACTTCAAT TTATCTCACT TCGTCCTCCA 
CCTCAAGCCG AAGCTCGTCG ACTTGCAGAA TCTCAAAAAG AGGTTTTTAT TGGTGTAGTC 
CCTTCTTTGC GTCGAAATCA TCTTTACAAT GACCTTATTT CTGCGCAAAT TAAATCATTC 
TCTTTTCTCG CCTATTTATT GAGAAGTTTT GGTGCTGCTC TTAAACAGTT TGAAAGCTCG 
ATCCCAATAT GTACCTTGCA ATTGTTCATG GACTGTCCTT CCGAATTATA TCAGACTCGA 
AGAGAACTTT TGGTAGCTAC AAGGCACGTC TTATCGACGG ATTATTTGAG AGGGTTTCTA 
CCGTATGTAG ATCAACTACT TGATACCAAA ATTCTAGTTG GTTCTGGAAT CACTTCTCAG 
CATTCATTAC GACCCATGGC TTTTAGCATG CTGGCAGATA TGCTTCATTA TGTGCGTATG 
GAATTAAGTC CACAACAAAT TTACAAAGTC ATACTTCTTT ATTTCAGTAT TTTGATGGAC 
GACTTTTATA CTTCAGCTAT TCAGGCAATG GCTACAAAGC TTATTTTAAA CTTGGTTGAA 
AGAATTGTTG CACTAGAAGA TTTTTCCACT AGCCGATCAC TTCTATTTGC TATACTTTTA 
TGTCTCTTAA GAAAATTGAC TTCTCTGAAT TTCGAGTTTA TGAAGCTGAG AGATTCTTTA 
CAGGAAAATG CTGACTTAAA GCAAATTAAA ATAGAAGAAA ATAAACATGA TCTTCCTATG 
TTTGAGAACC CTACTGGAGC TGCCCAACCT TCAGGTTTGG ATAAGCTAAA AGATTGCATA 
TTTCTTTTTA AAAACACCTT ATTAGGTATT AAGCCTGTTT TGTTTGGACT AAAGCAACGA 
AATATTCCTC TTGCCAACGG TTCCATTTTT ACAGCTCAGG AATGGTCTGA AAAGTTACAT 
CTTAGTTCAA CTAATGAAGT TTTACTGTTT CGAAGATTAC TTGTGGAAAG TTTAAAGGGA 
TTCAGTTATT ACCAGACAGA TGAAAAAACC GGTGTATTCA AGTCCTCGAA AAATCTTGCA 
TACTCTCAAC TTGATTCTTC GTTGACCACA AACCCTTCTA AACTCTTAGA AGAGAAGGAG 
CTCTTAGAAA TGTTAGCAAC TTTATTTTTA CACTTGGACC CATCTGTTTT CGTCGAAATT 
TTGGAATCCG AGTTTCCGAA TATATTTGAG TGTCTAGTAG ATAATCTTGC TTTATTGCAT 
ATTTTCCAAT TTTGGATGTC TAATGAAGTT ACTTCTGTCA ACTGCACGGG CATCGTGCTG 
TCTTTTTGCT GTGACAATCT AGCTAAAATA GGGTCTGGCC AATCCACTCG GGTTTCTGTT 
TTATTGCGAT TATTCAAGTT GGCCTTTATG ACTGTGAATG TTTTTCCCGA AAAAAATGCA 
GAAGTCCTGC GTCCCCATAT ATCCTATATC ATTTCAACTA GCCTCGAATT GACTACTGAT 
GCTGTTGAAC CTTTAAATTA CGTTTATTTA ATGAAAGCTT TGTTTAGAAA TATCAGTGGA 
GGAAAATTTG ATTCTCTTTA CAAGGAAATA CTACCACTTC TCCAAGTAAT GCTAGAATGT 
TTCAACAGAT TGATTTTTAC TGTTACGTCT ACATCCCAAA AAGAGCTGTA TGCGGAACTT 
TGTTTGACTT TACCCATTAG ACTCAGTGTG TTGTTGCCTC ATATGAATTT TTTAATGAAG 
CCATTGATTG TTGCATTAAA AGGCCCCCCT GAAATAGCTA GTCAAGGGCT TCGAATATTT 
GAACTTTGCT TGGATAATCT TACTCAAGAA TTTTTGGATC CTTTATTGGA CTCAATTATG 
CCGGATTTGC TGATATGCCT GTGGAATCAT TCACGCCTTA ATCAATCCAA TAATCAATTG 
CATCAAAGTG CTGTGAGAAT ATTAGGAAAA ATGGGTGGTC GAAATAGACA AATATATCTT 
GGTACCTTTG GCTTTGATTT TTTGCAAGAT GAAAATATTT TTCCTTCAAT CCAGTTTTCT 
TTCCAAGGCT CCTCTCAGAA TTTTAGCTTA GAGCACTCCA AATTTTTAAT GTCTTCATGT 
GCTGTTTTAA ACAATCAAAA TTCCGATTTA GAAGAAAAGA AGCAAGCATT TCAAATGGTC 
AAGAATTCGT ATTTATTACT CTTTGCGTCT GCGAAGCCCG ATGAAGACTT TTGGGAATCT 
ATTGATACAA TGTGCCGAGC AGTTGTAGAT CGCATGGATA AAAACTTACA GCAAGTCTCT 
AATGGTAGGT TGTGCCCTGA CAAAGATGAA TCTTATTATC TGCAAAGATC TATTGTCTCA 
AACATTTTTA AATCTTTAGT CGGCTCAATG TCTTGTGTGG AATTTGTAGC TGAAGCTCGA 
GAAACCATCA ATCGTTCTTT GGAATGGCTC ATTGTTCTTG ATTTGGTTAA TTACGCTGAC 
TCTCTGCAAA TAAAAGATCA AAATATTTTT GATAATTTGC AAAGCATTAA AATGCTTGAT 
CTTACTACGT GCATCAACGG AATTTTCGAA TCTTTATGTT CTGAAAATGA AAATACCAGG 
TCTAATGCTT TATCGTGTAT CGATCATTAT TTGAACGCCC ATAAGATGCT TCTCAACACA 
ACATTGGATA TATCAAAGTT GCCTAGTTTT CAAAATCTTG TAACAGTGTT TTGCCAAAGT 
TGCCATAAGG AACTGTGGTA CCAAAAGAAT GCTGGATTTC TCGGCTTAAA AGCAATACTT 
TCGTATGATT CACATCATAA GCTTTGGATC CAAGATAGAT TACACGACAT ATTGAAGGCT 
TTGTTCTTTA TTCTCAAGGA TACTCCAACC GATTATGGGG TTTTAAAATT AACCGAAGTT 
CGTTCTTTCA TCGTTGATAT TACAACTCAA TTCTGTATAC TTCAGGATGT GCTTGCACCG 
AAAGAGCGTG CTAATAATAT AATTAATGCA TTTTCTCCCT TTTTTTTAGA ACTCCTACAT 
CCCAACGATC ATGTTAGGAA TACTGTGCAA CAAGCTATTG AGAACATTTC CAACAATTCT 
AAACTTAGTG TGGTTGATCT TCTTCTTCCT ATAAAGGACA GGCTTTTAAG CCCTATTTTT 
GGAAAACCCT TAAGAGCGTT ACCTTTTACA ATCCAAATAG GGCATATAGA TGCAATCACT 
TACTGCTTGC ACCGCTCTCC CTCTTTTTTG GATTTGACTG ATGAATTGTA CAGATTATTT 
CGTGAAACAA TAGCATTAGC TGATGCTGAA GATGAAGCAT TAGTCACCAT GCTTAAAACT 
TCTCAAAGCA AAGATAGCTC TTCTTTACGA AAATTAAGGG CTACTTGCTT GCATTTACTA 
TTTGCTTCTT TGGTGGCTCA TAAGTTTGAT CAACCTCAAC ATGCTCAAAC TCGTACTAAA 
ATCATAGCGA TATTTTTCAA AGATCTATAC TCACCTCACA AAGAAATTTA TTCAGTTGCA 
ATAGATGCGT TAAGGCACGT GCTCTCGCAA AACCAAAAGC TTCCTAAAGA ACTTCTTCAA 
AGCGGCTTGA GACCAATTTT AATGAATTTA TCGGACCACA ATAAGCTGTC AGTTAACGGT 
CTTGAGGGTT TATCCAGACT TTTAAGATTA CTAACTAATT ACTTTAAAGT GGAAATAGGT 
CGGAAGCTTC TTCAACACCT TAATGTTCTA TCTGATTCTA AAGTCCTAGA GACTGCCTCA 
CTTTCGTTAT TGAAAACCAA CCCAAGGATC GAGATTATTG TGTCTTTGGT TAACGTATTC 
AGAGATCTTC CTCCGTTGTC AGCGCAGTTT CTAGGTGACT TGTTGTCGTC GGTCGTAAAC 
ATAGAGGCAG TTCTCAGAAA ATATTCTAAC AGCCCATTAA GAAAACCGTT GTATTCTTTT 
ATGGATTTGC ATGCAAATGA TACCTGGATG TATATTTTGA ATAATGCACG TAATGGCGAT 
TTAATAACTC GATTCGTTGG TGCTTTGAAT GACCCGATGT CTGAAAAACT TCGCGAAACC 
GCCGGTAATT ACTGGGGAAA GCTTTTGGAG CTAATTTCGC AACCGGTGTC TGTTGAAAAT 
TTGGCTCCAA TGTATGCTGT TGATATTATT GCAACCGTGT TTCCTTACAT TTCTGCCAAT 
GTAGATGCAG GAGTTATATC GGCTAAATTT ATTGTTTTAT CAAAGTCCTT GTATGGAATG 
CTTAGTTCAT ACAACGAATA TCTATTTCTT CCTATTCGCA GGTGTATATC GTCCATTACA 
AAAATGCTTT TATCTAGTTT AAAGAAAATA GAGAAGAAGC TCGAGTTTTC ATTAGAAGTT 
TTTAGATTTA AAGCTGACGA TGATAACGAT TTCCTTCCAG AATACATAGA TTCGTTATGT 
GGTTGTCTTA TAACCTCAAC AAGTGCAGCT GAAAAGAAAA GTATATTTTT AGTTTGCTGC 
AGTATAGTTG GTGACAAGTC AGTTCGACCT TTTTTTAAAG CATTTCTCTT GGATAAAGTA 
ATCAATCCGC TTGTATTTAA GTCATGCGGA GAAAATAACT TTATTGATAA GGATGTTGTT 
CATTCTGTTT ACACTCATGT TTGGCGTGTT TCAATTCGAG ATTTTGCTGA AGTATCGGGA 
GCCACAGATT CCTTTTACAT GGGAATTATG TGCTTGACAA CGGCGCTTTG TAAGTACCAT 
TCAGCACTTC TTAATGATTA TCGCAAGAGC GTTATAATGT CAGCTTGGAA CTACATTAAA 
CTCGAAGATC CTATGGTAAA ACAAGCCGCT TACGCTACGA TTGCATGTTT TATAAGTGCG 
TATGATACAC CTGCTAAGAT TGTGACACCT GTTTATGTTT CTATACTAAA AACATATCAA 
CCAGAAGTGA GAGCTTTTAT TGAATTCTCT CTTGCTTCTT TGCTGTCTGT ATTAACCGCT 
AGGCTATCGA GTCCTTCCGA TTCAACTTTC CCCCTTTGGG CGAAACTTCC TAGATTAGTT 
ATCTCCGAAG ATGTTCAGGG TATATCACAG CCTTTGACAG TTTACCAATT CATATGTAAA 
GCACCTGACC TTTTCTTCTC TTGTTGCTCT CATTTTATTG TACCCATGGT TAATGCGCTT 
CCAAAGCTTG TGTCGTTTTC TAGTGCTAGT ACAGAACCAC GAAAGTTAGC TTTGGATATT 
GTTCAAACCT TTATAAATTG GCAGAGGAAA CAGAATGAAT CAGAAAATTC CGAGACCACG 
CTTTTTAGCA ATTCCCATAT TGAAGCTATT CTTACCTTCC TTATAAAATT CCTTTCGCTT 
TTTCCCGAAC CAGTTGAAGA AAATCCACTT TCAAAGAAGG GGTTATCATT ATTTAATGAT 
CTGTTTAGTT TTCCTCGATG GAAAGACTGT CAGCTGAACT CTAACTTCTT TGAAAAAATC 
CTTGTTGATA TGGACTTCAA CGATAATAAC TATCGTACGG TAGCTAACAC ATTGTTTGTG 
TTTGGAGTTA TTTTAAAGAA CAGAGGTATG GAGTATATTC AACGTGAATA TAGTCATATA 
ATCGCTCTGA TCGACAAAAG TTTGCGTTGT GGTAAATTGC CTGTTACTCA TTCATTGGAG 
CAGATTATTT TATTACTGTT ACAAAGCCAT CCAACTCAAG CAGAAGAAGA AGAAGACACG 
AATGAGGCTG ATGATTTTAA ACAACTACTA CTTTCAGTTA TTCATGATAA TCTCGCCGCT 
GCCACTAACA TTGAGTCTGC TATTTGCTAT TTGCAAATTG TTAAATCCTC TAATCCGGAA 
GCGCTTGATG GTCTTCTTCT TCCCTTAAAT AAATGCTTTC AAAAGGTAGC ACGAGACCAT 
ATTGTCGCTT GCATGCAATC TGCTATACAA GCATCTGGAA AAGTTACACT TCCTTCTGCA 
TCTGATACTG TCTCAAAGCT TTTGATTAGT TTCATTGAAA TTATTAGAGT ACGCATGGCA 
AGCTTAGGAG ATCAACGTAG ATGGTTTTTA AGTGTTGTGG TGCAATTGCT CGAAAAGAGT 
TCAAGTTTTG AGCTTTGTGA ACATATTCTT AATGTTACAA AGGAATGGGT GATTGTGAAA 
AGAGATTCGT TTTTGACAGT CAAAGAAAAA ACTGCACTTT TATTGAAGAT GAGAACTTTT 
GAGGGAAGAT TTGACAACAA ACTATACATC GAAGCATGTG ATTTGCTATC CACGATATAC 
AGGGATCCCA TTTTTGCGCA TACAGAATTA ACTGCCCGAT TGAAGCAAGC CTTCCTTCTT 
GCTACGGCAT CTAAAGATAC CAAAATACGA ATGGATTTTA TGGATATTTT TGATTCCAGC 
ATGTCTCGTA ATGTTTATTC TAGATTGACT TTCATACTGG ATGCAACTAG CTGGGATACA 
ATTCCTAGCA TTTACTGGAT CAAACAAGCG AATTATATTC TTCTTGGGGC TATAAATGCG 
AAACAGCCTG TCCGGCTTAC CGACAATAGT CTTCGATTTG CTCCAGTGCC AATGACAAAA 
CCCATATTAA GCTCACTTCC TGAAGTTTTT TCAAAACACA ACGGTTCAGC TATTCCTCTT 
GGCAGATTTA CCTTTTTCAA ACAACTGGAT TTGTTCCTAA AAAGAAATAA GGAATTAACT 
GTGCAAAAAA TTATTTTTCC GTTGGCACAC ATTCAAATGC TCAGCGATGC AGATGCTAAT 
AAGCTTTGGC AATATATTTT TCCTCTTGCA TGGAAAATAC TAAGTTCGGA TAACCGTTCT 
GACCTTTCAA AGTCTTTAAT CTATTTACTC ACTCGTGATT ATCACATCAA ACAGGTAAAC 
AATCGACCGA ATGTGATCTC TACTTTGGTT TCATCTTTCG TTAAGTGTGC TGCAAAATTA 
GAGCTTCCAC CTCATTTGGT AAAATATCTT GGAAAATTGT ACGGTGTATA TCATGAATCC 
GTTTCACTTC TCGAAATTCA GCTGAGTGAT AAATATGACA TGTATCAGAA TGCAAAAGTT 
CAAGAAAGCC GTGCGGATGC AGTCGCAGAG TTATATGCAT CTTTAAATGA AGATGATATG 
TTTTATGGCC ATTGGCGACG AAACTGTAAG TACTTGCAAA CTCAAGTTGC TCTCTCGTAT 
GAGCAACTTG GAATGTGGGG AAGGGCTCAA CAACTTTATG AACAGGCACA GACCAAGGCT 
CGCAGTGAAG CCATTCCCTT CTCAGAATCA GAATACAACT TGTGGGAGGA TCAGTGGGTA 
ATGTGCGCTC AAAAATTACA ACAATGGGAC GTCTTGACAG AGTTGGCGAA GCACGAGGGA 
TCTTCTGAAC TATTGCTCGA GTGTGCTTGG AGAATTAGCG ATTGGTCTAA TAATCGTGAA 
TCCTTAGAGG TTGCCATTAA GAGCCTTTCT GATGTTCCTA CTCCACGGAA ACTTACCTTT 
CAATGCTTTA TGACACTTCA AAAGTCAGTA TCTCAACCTC TTGCCATTAA AGAGTTCCAA 
CAAGTATTGT CTGAAGCCAT TCAACTTGCG TTAATTAAGT GGCATCAACT TCCAGAAAAA 
GTTAATCAAT CTCATTATTC ACTTTTGCAT CTGTTTCAGC AATTTGTTGA ACTACAAGAA 
GCTGCTACAA TTTATTCACA TCTGAATGCT ATCAATTTTC AGAATTTGCC TACTAATGTT 
CAGTTGATTA AAAGTGCTCT TCAAGTTTGG CAAGAGCGGT TACCTAATGT CTGGGACGAT 
ATTAATCTTT GGCGTGATCT CATTTCTTGG AGACAAATAG TATTCTCTAT GATAAATCGT 
GTTTATCTCC CCTTGGTCCC TACGATACAG GCTAATAGTA GTGCTGATTC GTCTAATCCT 
CCGAATACCT CTTTCTTATT TAGAGGATAT CATGAAACCG CTTGGCTAAT TAACCGATTC 
GCTCATGTTG CCAGAAAACA TAAACTACCT TCCGTATGTC TGAACCAATT AACGAAGATA 
TATACTCTTC CAAATATTGA AATTCAAGAA GCTTTTTATA AGCTTCGAGA GCAAGTTTTA 
TGTTATCTAC AAAACCCAAG AGATCTGAAA ACAGGTTTGG AGGTTGTTAC AAATACCAAT 
CTGATGTACT TCAATTCTAG ACAAAAGTCA GAATTCGTGA CATTAAAAGG AAAATTTTTA 
GAAAAACTAA ATCGAGGTGA GGAGGCTAAC CAAATGTATG CTGCAGCAGT ACAAATTGAT 
TTAGGGTTGC CAAAGGCATG GGCAGAATGG GGTCGTTACA ACGATTTGCT TTTCAATAAG 
TCACCAGATA ATTTGAGTGC AGCATGCAAT GCCATTAGTT GCTATCTTCA AGCTGCTGGT 
ACTTACCAAA GTTCTAAAGC TCGTAAGATG CTGGCAAGGG TATTGTGGTT ATTAAGTTTG 
AATGACAGCG CCGGTACTAT AGTTAAAGCT TTTGAATCAT ACAAAGGTGA TATACCAGTT 
TGGCATTGGC TTACCTTTAT TCCCCAGCTT TTAAATTCAC TTTCTAAAGG GGATACTAAA 
TGCGCACCTG TTGTTCTTAA AAAAATTGCT AAATCCTATC CACAGGCACT ATTTTTCACT 
TTAAGAACTG CTCGTGAAGA TATTGCACAG GTAAAACGCA CTGAGATGGC AGCATGGAAA 
TCAAATACGA CTGATGAAAA TAAAAGAAAT GAGGATATTT TAAGCATACA ATCTAACTTT 
CTTTCTACCA ATCAAAGCAC GAATGCTCCA GCAACAAATA AAGAAGATGG ACTTTCTAAA 
AAAGTTTGGG AATATATTGA TGAAATTATG TCAATTTTAA AAACTGCTTA TCCTCTATTG 
GCTTTGACTA TGGAAACTTT AGTTGATCAA ATTCAAGCGA AGTTTAAATG TAAGAATGAT 
GGAGATGCTT TCAGGCTAGT GGTAGCCTTG CTTAATGATG CTGTACAGCA TAGCATTCGT 
TTGGGGATCG TAACGGATGA AATGAAACTT CCTTCTTCAA CCGAATCGAA CTTAAGTTTG 
TTTGCTGATA ATATTCTCCC TGACTATTGT AAACAACTAT TTAAGGAAGA TTTCATTGTC 
AATTCAAACG GTTTGAAATC ATATATTTTC AAACTAAGGA AATGGAGATC ATATTTTGAG 
CGGTTATTAT CTAAGGTTCC TAAAAAACAA TATTTGGAAC AATACAGCTC ATTCTTGTGT 
GAATTTCATC ATCAAAAATT TGATGAAATA GAAGTTCCTG GACAATATCT TTTGCACAAG 
GATAACAACA ATAGTTTTTC TTGCATAGAA AGGTTTCTTC CTGAAGTGGA ATTGATAGTA 
GGACATGGCG TTTGCTATAG AAGGTTATCC ATTCGAAGCA ATGGCGGAAC TATACACCCA 
TTTGTCATTC AGTATCCTTC AGCACGAAAC AGCCGTAGGG AAGAGCGTTT TATGCAGCTG 
ACCAGATACT TAAATGATGC ACTTGCTTTA AATTGTGAGA CTCGAAGACG TTGTTTGAAA 
TTTTACATTC CTGCAGTGAT TCCGTTGTCA TCGCACATCC GGCTGTTAGA AGACCAACCG 
TCTTCTATTA CTCTCCAAAA AATATACGAA ATTTATTCAG AAAGAAATAA CTTCTCCAGA 
GATGATCCGA AAGAGTTGTT TACAAACGAG TTATCAAAAC ATATGATGGA ATTAAACTCC 
CAAATTAGTC AGGAATCTAC TGATGCTGAA AAATTAGCTA ATCGTAAACA ATTGTTTAGT 
AGGAGAATCG GAATGTTTGA AAATATTCAA AAGCTTTATT CTCCGTCAAC GATTTTAAAA 
GATTATTTCT CAAGTATCTT TACTAATTAT TCCGATCTGT GGTTGTTCAG AAAAAATTTT 
TCATACCAAT ATGCTTGTTT TTCATTTATT ACATACATAC TAAGTATAAA TAATAGGATA 
CCTGCTAAGC TTGTTTTTTC TAGAGACTCT GGCGGTGTTT GGACAACAGA AGCGCTGCCA 
TCTATGGTTT CTTCTACTCC AGTTTACCAT AATGGAGAAA TTGTTCCTTT TCGCTTTACC 
CCCAACATTA AAGAATTTAT TGGAAAAACT TGTACTGAAG GTTTACTGGG CCCTTCAATA 
ATGGCTATAG CTCGCGCGCT CTCCAAACCG GATTTCGATT TGGATATGTA CTTGGGTATC 
TTTATTAGAG ATGATCTTTT TTGGTGGCTT GCACAGCAAA CTAAAGGCGT GCCGGCAGAT 
TTCAGTATGC TCAACAAAGT CAATAGCAAT ACTGATCTTA TAATGCGAAG GGTTGCATCA 
TTAAGTCAGG TTGCTTATGG TAACTTACCA GTCAATCAAA CAGCTATTGA TTATTTGGCG 
CAAGCCTCTA GTTCCAAAGT TTTGGCACAG ATGGACGTCT TATGGGCTCC TTGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tra1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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