hsk1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	hsk1
Entrez Gene ID	2541165
Full Name	Dbf4(Dfp1)-dependent protein kinase Hsk1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022249.2	NP_596328.1	Dbf4(Dfp1)-dependent protein kinase Hsk1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5790	NP_609876.2
Saccharomyces cerevisiae (baker's yeast)	CDC7	NP_010267.3
Eremothecium gossypii	AGOS_AER216C	NP_985073.1
Magnaporthe oryzae (rice blast fungus)	MGG_03488	XP_003716435.1
Neurospora crassa	NCU11410	XP_001728522.1
Kluyveromyces lactis	KLLA0C04345g	XP_452391.1
Schizosaccharomyces pombe (fission yeast)	hsk1	NP_596328.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

REVIEW: Hsk1/Cdc7, with its unique target specificity, can now be regarded as an important modulator of various chromosome transactions
Title: Regulation of chromosome dynamics by Hsk1/Cdc7 kinase.

Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation
Title: Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.

Hsk1 kinase activity is required for induction and maintenance of Mrc1 hyperphosphorylation, which is induced by replication fork block and mediated by Rad3.
Title: Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.

Hsk1-Dfp1 kinase functions post-initiation to maintain replication fork stability, an activity potentially mediated by the C terminus of Dfp1.
Title: Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

propose that Hsk1 plays a vital role during post S phase in genome stability via SCF(Pof3)-mediated degradation of Ams2, thereby maintaining centromere integrity
Title: Hsk1- and SCF(Pof3)-dependent proteolysis of S. pombe Ams2 ensures histone homeostasis and centromere function.

The following hsk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hsk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101108	NM_001022249.2 Latest version!	Schizosaccharomyces pombe Dbf4(Dfp1)-dependent protein kinase Hsk1 (hsk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hsk1 ( NM_001022249.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596328.1 Schizosaccharomyces pombe Dbf4(Dfp1)-dependent protein kinase Hsk1 (hsk1), mRNA.

Price & Availability↑

CloneID	OSc101108
Clone ID Related Accession (Same CDS sequence)	NM_001022249.2
Accession Version	NM_001022249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Dbf4(Dfp1)-dependent protein kinase Hsk1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022249.1.

ATGGCAGAGG CCCATATCAC ACTATCACCT AAAGTAACTC ATGAACAACA AACAGATATT 
GATTCTGAAT GTGAAATCAC TGAGGTGGAC GATGAAAATG TAAACGAAAA CAAATCTCAA 
GAAATGATTC AAGATATCCC TGCACGAGAC CGCGAAGAAA TTGAAAATAT CACAAGGACG 
TTCGTAGAGC TACAAGAGAA CTATCGGCTA ATTGAAAAAA TTGGAGAAGG TACGTTCTCC 
AGTGTATATA AAGCGGAAGA CCTACATTAT GGGAGGTATA TTAATGATTG GGACATTCAA 
AGTGAAGTGT TAAAGGAATC TTCTTTTGGA AAAGAAAAAA TTCCTGTTAA TGAAGACAGC 
CGGAAACCGA AGTACGTTGC TATAAAAAAA ATTTACGCGA CAAGTAGTCC TGCTAGAATA 
TACAATGAAT TAGAAATCCT ATATTTATTA AGGGGATCGT CGGTAATAGC CCCGCTAATA 
ACTGCTTTAA GAAATGAAGA TCAGGTACTC GTAGTTTTAC CCTACTACGA GCATACTGAC 
TTTCGTCAAT ATTATAGCAC TTTTTCCTAT CGCGATATGA GCATTTATTT TCGATGCCTT 
TTTCAAGCTA TGCAGCAAAC TCAGACTTTG GGAATCATTC ATAGAGACAT TAAGCCTAGT 
AACTTTCTCT TCGATGTTCG TACCAAGCAT GGTGTCTTAG TTGACTTTGG ATTAGCAGAG 
CGTTATGATG GAAGACAACA ATCACATTCT TGCCGGTGCA CTAATTCAAA TGCGGCCGAA 
TTAGCACATG ATTTTTCGAT TGCGCAGGAA ACATCTCTTG GATACATAAA AAACGATACT 
CGACCAAGCA AACGGGCCAA TAGAGCCGGA ACTCGAGGAT TTCGAGCACC TGAAGTATTG 
TTTAAATGCT CATCTCAATC ACCCAAAGTT GATATTTGGT CTGCTGGGGT TATTTTGTTA 
TCTTTCCTTA CTAAACGTTT CCCCATGTTT AATTCAAAGG ACGACGTGGA TGCACTCATG 
GAGATAGCAT GTATATTTGG AAAAAGCGAA ATGCGCCAAT GTGCTGCTTT ACACGGTTGT 
ACGTTTGAGA CAAATGTAAG CACGCTTACA GAAAAAAGAG TAAACTTTAG GAAACTTATT 
TTGTGGGCCA GTTGTGGTTC AGCCAGTATA TACAAAGAAA AGCTTCGTCA TAAACCAAGC 
CAAGAAGAGC GATTGTGCCT GGATTTTTTA GAGAAATGTC TTGAACTTGA CTGTAACAAA 
AGGATTTCAG CTGAGGAAGC ATTGGATCAC GATTTCTTGT ATCTCGATAA TTTAGCTTAC 
GAAAAGAAAG ATGACGATAC AGCTTTTGAT AATAGCTTTG GAGAAACCTC TTTCGAGAAA 
GACGAAGACT TAACTGCTAA GCACTTATCC CATATCCTTG ATTTTAAAGA GCAGGAAGAA 
ACAGATGAAC CTACTTCTTT ATCAAAGCGG AAGCGAAGTA TTGATGAAAT TTTACCAAAT 
GATGCTTTGC AGGATGGAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596328.1
CDS	259..1782
Translation	MAEAHITLSPKVTHEQQTDIDSECEITEVDDENVNENKSQEMIQDIPARDREEIENITRTFVELQENYRLIEKIGEGTFSSVYKAEDLHYGRYINDWDIQSEVLKESSFGKEKIPVNEDSRKPKYVAIKKIYATSSPARIYNELEILYLLRGSSVIAPLITALRNEDQVLVVLPYYEHTDFRQYYSTFSYRDMSIYFRCLFQAMQQTQTLGIIHRDIKPSNFLFDVRTKHGVLVDFGLAERYDGRQQSHSCRCTNSNAAELAHDFSIAQETSLGYIKNDTRPSKRANRAGTRGFRAPEVLFKCSSQSPKVDIWSAGVILLSFLTKRFPMFNSKDDVDALMEIACIFGKSEMRQCAALHGCTFETNVSTLTEKRVNFRKLILWASCGSASIYKEKLRHKPSQEERLCLDFLEKCLELDCNKRISAEEALDHDFLYLDNLAYEKKDDDTAFDNSFGETSFEKDEDLTAKHLSHILDFKEQEETDEPTSLSKRKRSIDEILPNDALQDGA

Target ORF information:

RefSeq Version	NM_001022249.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Dbf4(Dfp1)-dependent protein kinase Hsk1 (hsk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022249.2

ORF Insert Sequence:

ATGGCAGAGG CCCATATCAC ACTATCACCT AAAGTAACTC ATGAACAACA AACAGATATT 
GATTCTGAAT GTGAAATCAC TGAGGTGGAC GATGAAAATG TAAACGAAAA CAAATCTCAA 
GAAATGATTC AAGATATCCC TGCACGAGAC CGCGAAGAAA TTGAAAATAT CACAAGGACG 
TTCGTAGAGC TACAAGAGAA CTATCGGCTA ATTGAAAAAA TTGGAGAAGG TACGTTCTCC 
AGTGTATATA AAGCGGAAGA CCTACATTAT GGGAGGTATA TTAATGATTG GGACATTCAA 
AGTGAAGTGT TAAAGGAATC TTCTTTTGGA AAAGAAAAAA TTCCTGTTAA TGAAGACAGC 
CGGAAACCGA AGTACGTTGC TATAAAAAAA ATTTACGCGA CAAGTAGTCC TGCTAGAATA 
TACAATGAAT TAGAAATCCT ATATTTATTA AGGGGATCGT CGGTAATAGC CCCGCTAATA 
ACTGCTTTAA GAAATGAAGA TCAGGTACTC GTAGTTTTAC CCTACTACGA GCATACTGAC 
TTTCGTCAAT ATTATAGCAC TTTTTCCTAT CGCGATATGA GCATTTATTT TCGATGCCTT 
TTTCAAGCTA TGCAGCAAAC TCAGACTTTG GGAATCATTC ATAGAGACAT TAAGCCTAGT 
AACTTTCTCT TCGATGTTCG TACCAAGCAT GGTGTCTTAG TTGACTTTGG ATTAGCAGAG 
CGTTATGATG GAAGACAACA ATCACATTCT TGCCGGTGCA CTAATTCAAA TGCGGCCGAA 
TTAGCACATG ATTTTTCGAT TGCGCAGGAA ACATCTCTTG GATACATAAA AAACGATACT 
CGACCAAGCA AACGGGCCAA TAGAGCCGGA ACTCGAGGAT TTCGAGCACC TGAAGTATTG 
TTTAAATGCT CATCTCAATC ACCCAAAGTT GATATTTGGT CTGCTGGGGT TATTTTGTTA 
TCTTTCCTTA CTAAACGTTT CCCCATGTTT AATTCAAAGG ACGACGTGGA TGCACTCATG 
GAGATAGCAT GTATATTTGG AAAAAGCGAA ATGCGCCAAT GTGCTGCTTT ACACGGTTGT 
ACGTTTGAGA CAAATGTAAG CACGCTTACA GAAAAAAGAG TAAACTTTAG GAAACTTATT 
TTGTGGGCCA GTTGTGGTTC AGCCAGTATA TACAAAGAAA AGCTTCGTCA TAAACCAAGC 
CAAGAAGAGC GATTGTGCCT GGATTTTTTA GAGAAATGTC TTGAACTTGA CTGTAACAAA 
AGGATTTCAG CTGAGGAAGC ATTGGATCAC GATTTCTTGT ATCTCGATAA TTTAGCTTAC 
GAAAAGAAAG ATGACGATAC AGCTTTTGAT AATAGCTTTG GAGAAACCTC TTTCGAGAAA 
GACGAAGACT TAACTGCTAA GCACTTATCC CATATCCTTG ATTTTAAAGA GCAGGAAGAA 
ACAGATGAAC CTACTTCTTT ATCAAAGCGG AAGCGAAGTA TTGATGAAAT TTTACCAAAT 
GATGCTTTGC AGGATGGAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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hsk1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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