nuc1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	nuc1
Entrez Gene ID	2540873
Full Name	DNA-directed RNA polymerase I complex large subunit Nuc1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022221.2	NP_596300.1	DNA-directed RNA polymerase I complex large subunit Nuc1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nuc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nuc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100304	NM_001022221.2 Latest version!	Schizosaccharomyces pombe DNA-directed RNA polymerase I complex large subunit Nuc1 (nuc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nuc1 ( NM_001022221.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596300.1 Schizosaccharomyces pombe DNA-directed RNA polymerase I complex large subunit Nuc1 (nuc1), mRNA.

Price & Availability↑

CloneID	OSc100304
Clone ID Related Accession (Same CDS sequence)	NM_001022221.2
Accession Version	NM_001022221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA-directed RNA polymerase I complex large subunit Nuc1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022221.1.

ATGAATATTG CACAGCCAGT CTCTTCGGAG ATTAAAAGTG TGAAGTTTGG GATTTACGAT 
GTTGACGATG TCGAAAAAAT ATCCGTCAAA CAAATCGTAA ATCCCGTTTT GCTTGACAAT 
TTAAATCACC CTACTAACGG CGGATTATAT GACCTTGCTT TGGGTCCATA TCTAAAAAAT 
TCTGTTTGCG CTACATGTCA TTTGGATGAA AGATACTGCC CTGGTCATTT TGGTCATATA 
GTACTTCCTA TTCCTGCATA TCACCCTCTA TTTTTTTCTC AAATGTACAA TTTATTACGT 
TCAACTTGCT TGTACTGCCA TCATTTCAAG CTTTCAAAAG TCAAGGTTCA TCTTTTCTTT 
TGTAGACTCA AGTTGCTTGA TTATGGTCTT TTAAATGAGT CAGAGATGGT GGAGAATGTC 
AGTCTTACTG AGGCAATCAT AAAGAATTCT AACGGCACTC CACTTGAAGA TGGAAGTGAC 
AGCGAGGACA GTGGTCTTGG ACACGATGAT ATTGCTAAAG ATGCTGCAAC ACTTATGCGC 
ATTCGCGATG AATTCGTTGC TAAGTCTATT GCTGACTCAC GCCAGAACGC TCATATCGAT 
GCTCAGTTGA CTACACTTCT TTTACACGAG AGGAAAAAAG TTGTGCGTGC CTTCTACCAT 
GCCATTTCAT CTCGCAAACA ATGTGATAAT TGCCAGTCTT TTTCCCCTAA CTTTAGGAAA 
GAAGGCTTTG CTAAAATATT CGAGATTCCA TTGAGTGGAA AAAATCTCCA ATTTATGGAG 
CAAACCGGAA AAATTCGTTC TGACGTTCTT CGTGATACTA GTAAAAAGCA TCATGAAGAT 
GAGGGATATG ATGGAGATTC TGATTCGAGT AATGAAAGTG AAGTAGAAGG AATAGATCTC 
TTTGAAGAAG ATCCTAATCC TTTGAAAAAT AAATCAAAAT CTCCAATTGC TCATGGAGCC 
AAATATATGA CTTCTACTGA AGTCCGTAAC CATCTTCGCC GTCTTTTTGT AAAAGAAAAT 
GTTGTCCTTT CTCGTCTTTA TGCTCATAAA CGCGGAAAAC CTGCCTCTGC TGATATGTTT 
TTCCTCCAAA ATATTGCTGT TCCTCCTACT CGCTTCCGTC CAGCTTCCAA AATGGGCGAT 
GAAGTACATG AAAATATCCA AAATGAACTT CTGACCAGAA TTTTACAATC CTCAATACAA 
ATTGCTTCTC TCTCTAAAGA CTCTACAGTT GAAGTAAATC CTGATGAGAA AGAAGGCTTG 
GAGCGTCGTT CTCGTGCTTT TGAACTGTTG ATTAACGCTT TTGTTCAACT ACAACACGAT 
GTAAACAGCC TGATTGATAG TAATAGGAAT CCTAGTTCTG GAGGACAGAG TAGAACAGTA 
CCCCCGGGTA TTAAGCAAAT TTTGGAAAAG AAGGAGGGTC TTTTCCGTAA GCATATGATG 
GGTAAACGTG TGAATTATGC AGCTAGATCT GTCATTTCAC CTGACCCGAA CATTGAAACT 
AATGAAATCG GTGTTCCGCC AGTCTTTGCT ACTAAACTCA CATACCCAGA ACCAGTAACT 
CTTTACAACT TTAACGAGAT GAGAAATGCG GTTATTAATG GTCCCCATAA ATGGCCTGGT 
GCTAGTCATA TTCAAAACGA AGACGGCACT TTAATTTCTT TAATGCCTTT AACAATTGAA 
CAACGCACTG CTTTAGCCAA CCAGCTTCTT ACACCTCAAA GCAATTTAAT ATCTTCCCCA 
TATTCTTATT CTCGCTTAAT AAATACTAAC AAAAAGGTTT ATCGTCATGT CAGAAATGGA 
GACATGTTGA TATTGAACCG TCAACCCACC CTTCATAAGC CTTCGATGAT GGCTCATAAA 
GCTCGTATTC TTCCAGGTGA GAAGACGATT AGAATGCATT ACGCAAATTG CAACTCTTAC 
AATGCTGATT TCGATGGCGA CGAAATGAAC ATGCATTTTC CACAAAGTAC CAATGCGCGT 
TCTGAGGCTC AATTTATTGC AAACACAGAC TCTCAATATT TGGTGCCTAC CTCTGGTGAT 
CCTTTGCGCG GTCTTATTCA AGACCATGTC GTAATGGGTG TTTGGCTTAC CTGTAAAGAT 
ACATTTTATA CTCGAGATGA ATATCAGCAA CTTCTATTTC AGGCTTTAAA GCCTGATGAA 
ACCGGTATGT ATGGGCGTAT CAAAACTCTT CCTCCTGCCA TACAACGTCC GGGTATATAC 
TGGACAGGTA AACAAATAAT ATCTTCTGTT TTGCTCAACC TAAAACCCAG TGATCGTCCT 
GGTCTTAATC TTAAAAGCAA AGCAAAGGTT CCAGGAAAAT ACTGGTCACC TGACTCAGAG 
GAGGGATCAG TATTATTTGA TGATGGTGAA CTTTTATGCG GTATTCTTGA TAAGTCCTCT 
TTTGGTGCCT CGGCTTTTGG CTTAGTTCAC TCAGTTCATG AATTGTACGG TCCTGACATC 
GCTGGTCGCT TGCTTTCTGT TTTAAGTCGT CTATTCACTG CGTATGCTCA AATGCGGGGC 
TTTACTTGCC GTATGGATGA TTTGAGACTT GATGAGCAAG GTGATAATTG GAGAAGACAA 
CTATTGGAAA ACGGAAAATC CTTTGGACTT GAAGCTGCCA GTGAATATGT TGGCCTTTCA 
ACAGATTCGC CAATTGCGCT TTTGAACGCT AACCTTGAGG AAGTTTACAG AGATGATGAG 
AAGTTACAAG GTTTAGATGC TGCTATGAAA GGTAAAATGA ACGGGCTTAC TTCCTCAATT 
ATCAACAAAT GTATCCCTGA TGGTTTACTT ACAAAATTCC CCTATAATCA TATGCAAACT 
ATGACCGTTT CTGGTGCCAA GGGTAGCAAC GTAAACGTCT CACAGATTTC TTGTCTTTTG 
GGTCAACAGG AATTAGAAGG ACGTCGTGTT CCTCTTATGG TGAGCGGAAA GTCACTTCCT 
TCCTTTGTTC CATATGAAAC TTCTGCTAAG TCTGGTGGTT TTATTGCCAG CAGATTTTTA 
ACTGGTATCG CCCCTCAGGA GTATTATTTT CACTGTATGG CTGGCCGTGA AGGCTTGATT 
GATACCGCTG TTAAAACCAG TCGGTCTGGT TATCTTCAGC GTTGTCTGAT GAAGCATCTG 
GAAGGTTTAT GTGTACAATA TGATCATACT GTTCGCGATT CCGATGGATC TATCGTCCAA 
TTCCATTACG GAGAAGATTC TTTAGATGTC ACAAAACAGA AGCACTTGAC ACAATTTGAG 
TTTTCAGCCA AAAATTATAA ATCCTTAATT CAGAAATACA AGGTCAAATC TGTCTTATCG 
GCGGTTGATT CCGAAACTGC ATCCTCTTAC GCGAAGAAAG CGTTGAAAAA GCCGTACAAA 
TATGATCCAG TATTGGACAA GTATCCCCCT AGTCGATATT TGGGAAGCGT AAGCGAGAAG 
TTTCAACGTG CTGTTGATGA ATATACCCAG AAAAATCCGG ACAAGTTGAT TGCTTCGAAG 
AAAGAGAGCA AGCTTGATGA TTCTCTTCTT AATGAATCAA AGTTCAAGGC ATTGATGCAG 
TTACGTTACC AACAGTCTCT GGTTGATCCT GGTGAAAGTG TTGGTGTATT AGCTAGTCAA 
TCAATCGGTG AGCCATCTAC TCAAATGACT TTGAACACAT TCCATTTTGC TGGATTCGGT 
GCTAAGAATG TCACATTAGG TATTCCTCGT TTACGTGAGA TTATCATGAC CGCTAGTGCA 
AATATTCAAA CTCCTACGAT GACTCTTCGT TTGAATGACG GCGTTTCCGA TAAAAGAGCT 
TCAGCTTTTT GTAAAGAAGT AAATAAATTG GTTTTATCTG AAGTTGTTCG ACAAGTCCGA 
GTAACTGAAA AAATTTCTGG TCAAGGCTCT GACGAACAAT CTAAAACTTA TGCTATTCGA 
CTTGATTTAT ATTCTCGTGA TGAATATCAG GATGAATATG GTGTTCTTCA GGAAGAGATT 
GAGTCGACCT TTAGTAATAG ATTCTTAAAA ATTCTTAACC GAATTATTAA AAGCTATTTG 
GCTAAATCTA AGCAGCGGAA GTCAGGAGGT AAAGATGATA CAGTACCTGA GGTTGGTCAG 
GCATTAAAGC CACTTGAAGA TATCGATGAA GCACCCATTG AAGGTCGTGC TCAAGAAGCT 
CTCGAAGATG AAGACAATGA TGCTACCAAT GAGAAAATGG TCTCTAGGTC GAAACAGCAT 
GCTTCTTATG AGGGGCCTGA TGAGGCAGAC AAAGTCGCCC TTCGTCAGCT AAAAGGATCA 
AATAAAGTTG AAGACGTAAA CATGGATGAA GAGGAAGATG AGGGTTTCAA GTCCGATGAA 
AGTGTCTCTG ATTTTAAAGA AAGGAAATTG TTGGAAAAGC AGAACACAGT TTCCATTTCG 
GAAAGGCGGG AACTTCAGTT AAAGACAGCA AAAGAAATAC TCAGCAATTG CAAACACCTC 
GACTTTGATT ACGTTAACGG TGAGTGGGCA ACTGTTGAAT TGGTGTTCCC AATCAATACT 
GAAAAGCTAT TAATGGTGTC ACTTGTGGAA AAGGCATGTT CCGAAACTGT CATACACGAA 
ATTCCGGGCA TTACACGCTG TTTTTCCAAA CCTCCAGACT CTGCCTTAGA CACTGTTCCG 
AAGGTTATCA CTGAAGGTGT CAATCTGAAA GCAATTTGGG AATTTTACAA TGAAATCTCC 
ATGAATGATA TTTACACAAA TGATATTGCA GCGATCCTTA GGATATACGG TGTTGAAGCA 
GCTCGTAATG CGATTGTTCA TGAGGTTTCC TCCGTTTTTG GAGTGTATGG TATTGCTGTT 
GATCCTCGTC ATCTTTCCTT AATTGCAGAT TATATGACTT TCGAGGGAGG ATACAAAGCC 
TTCAATAGAA TGGGAATAGA ATATAACACT TCTCCATTTG CGAAAATGTC TTTTGAGACC 
ACTTGTCACT TTTTGACTGA GGCCGCCCTC CGTGGTGATG TTGATGACTT ATCCAATCCT 
TCATCTCGGT TAGTCGTTGG TCGAGTCGGT AACTTTGGCA CCGGTTCTTT TGACATATTC 
ACCCCTGTAG TCGATTCTCC TGCAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596300.1
CDS	124..5193
Translation	MNIAQPVSSEIKSVKFGIYDVDDVEKISVKQIVNPVLLDNLNHPTNGGLYDLALGPYLKNSVCATCHLDERYCPGHFGHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVKVHLFFCRLKLLDYGLLNESEMVENVSLTEAIIKNSNGTPLEDGSDSEDSGLGHDDIAKDAATLMRIRDEFVAKSIADSRQNAHIDAQLTTLLLHERKKVVRAFYHAISSRKQCDNCQSFSPNFRKEGFAKIFEIPLSGKNLQFMEQTGKIRSDVLRDTSKKHHEDEGYDGDSDSSNESEVEGIDLFEEDPNPLKNKSKSPIAHGAKYMTSTEVRNHLRRLFVKENVVLSRLYAHKRGKPASADMFFLQNIAVPPTRFRPASKMGDEVHENIQNELLTRILQSSIQIASLSKDSTVEVNPDEKEGLERRSRAFELLINAFVQLQHDVNSLIDSNRNPSSGGQSRTVPPGIKQILEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFATKLTYPEPVTLYNFNEMRNAVINGPHKWPGASHIQNEDGTLISLMPLTIEQRTALANQLLTPQSNLISSPYSYSRLINTNKKVYRHVRNGDMLILNRQPTLHKPSMMAHKARILPGEKTIRMHYANCNSYNADFDGDEMNMHFPQSTNARSEAQFIANTDSQYLVPTSGDPLRGLIQDHVVMGVWLTCKDTFYTRDEYQQLLFQALKPDETGMYGRIKTLPPAIQRPGIYWTGKQIISSVLLNLKPSDRPGLNLKSKAKVPGKYWSPDSEEGSVLFDDGELLCGILDKSSFGASAFGLVHSVHELYGPDIAGRLLSVLSRLFTAYAQMRGFTCRMDDLRLDEQGDNWRRQLLENGKSFGLEAASEYVGLSTDSPIALLNANLEEVYRDDEKLQGLDAAMKGKMNGLTSSIINKCIPDGLLTKFPYNHMQTMTVSGAKGSNVNVSQISCLLGQQELEGRRVPLMVSGKSLPSFVPYETSAKSGGFIASRFLTGIAPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLMKHLEGLCVQYDHTVRDSDGSIVQFHYGEDSLDVTKQKHLTQFEFSAKNYKSLIQKYKVKSVLSAVDSETASSYAKKALKKPYKYDPVLDKYPPSRYLGSVSEKFQRAVDEYTQKNPDKLIASKKESKLDDSLLNESKFKALMQLRYQQSLVDPGESVGVLASQSIGEPSTQMTLNTFHFAGFGAKNVTLGIPRLREIIMTASANIQTPTMTLRLNDGVSDKRASAFCKEVNKLVLSEVVRQVRVTEKISGQGSDEQSKTYAIRLDLYSRDEYQDEYGVLQEEIESTFSNRFLKILNRIIKSYLAKSKQRKSGGKDDTVPEVGQALKPLEDIDEAPIEGRAQEALEDEDNDATNEKMVSRSKQHASYEGPDEADKVALRQLKGSNKVEDVNMDEEEDEGFKSDESVSDFKERKLLEKQNTVSISERRELQLKTAKEILSNCKHLDFDYVNGEWATVELVFPINTEKLLMVSLVEKACSETVIHEIPGITRCFSKPPDSALDTVPKVITEGVNLKAIWEFYNEISMNDIYTNDIAAILRIYGVEAARNAIVHEVSSVFGVYGIAVDPRHLSLIADYMTFEGGYKAFNRMGIEYNTSPFAKMSFETTCHFLTEAALRGDVDDLSNPSSRLVVGRVGNFGTGSFDIFTPVVDSPAN

Target ORF information:

RefSeq Version	NM_001022221.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA-directed RNA polymerase I complex large subunit Nuc1 (nuc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022221.2

ORF Insert Sequence:

ATGAATATTG CACAGCCAGT CTCTTCGGAG ATTAAAAGTG TGAAGTTTGG GATTTACGAT 
GTTGACGATG TCGAAAAAAT ATCCGTCAAA CAAATCGTAA ATCCCGTTTT GCTTGACAAT 
TTAAATCACC CTACTAACGG CGGATTATAT GACCTTGCTT TGGGTCCATA TCTAAAAAAT 
TCTGTTTGCG CTACATGTCA TTTGGATGAA AGATACTGCC CTGGTCATTT TGGTCATATA 
GTACTTCCTA TTCCTGCATA TCACCCTCTA TTTTTTTCTC AAATGTACAA TTTATTACGT 
TCAACTTGCT TGTACTGCCA TCATTTCAAG CTTTCAAAAG TCAAGGTTCA TCTTTTCTTT 
TGTAGACTCA AGTTGCTTGA TTATGGTCTT TTAAATGAGT CAGAGATGGT GGAGAATGTC 
AGTCTTACTG AGGCAATCAT AAAGAATTCT AACGGCACTC CACTTGAAGA TGGAAGTGAC 
AGCGAGGACA GTGGTCTTGG ACACGATGAT ATTGCTAAAG ATGCTGCAAC ACTTATGCGC 
ATTCGCGATG AATTCGTTGC TAAGTCTATT GCTGACTCAC GCCAGAACGC TCATATCGAT 
GCTCAGTTGA CTACACTTCT TTTACACGAG AGGAAAAAAG TTGTGCGTGC CTTCTACCAT 
GCCATTTCAT CTCGCAAACA ATGTGATAAT TGCCAGTCTT TTTCCCCTAA CTTTAGGAAA 
GAAGGCTTTG CTAAAATATT CGAGATTCCA TTGAGTGGAA AAAATCTCCA ATTTATGGAG 
CAAACCGGAA AAATTCGTTC TGACGTTCTT CGTGATACTA GTAAAAAGCA TCATGAAGAT 
GAGGGATATG ATGGAGATTC TGATTCGAGT AATGAAAGTG AAGTAGAAGG AATAGATCTC 
TTTGAAGAAG ATCCTAATCC TTTGAAAAAT AAATCAAAAT CTCCAATTGC TCATGGAGCC 
AAATATATGA CTTCTACTGA AGTCCGTAAC CATCTTCGCC GTCTTTTTGT AAAAGAAAAT 
GTTGTCCTTT CTCGTCTTTA TGCTCATAAA CGCGGAAAAC CTGCCTCTGC TGATATGTTT 
TTCCTCCAAA ATATTGCTGT TCCTCCTACT CGCTTCCGTC CAGCTTCCAA AATGGGCGAT 
GAAGTACATG AAAATATCCA AAATGAACTT CTGACCAGAA TTTTACAATC CTCAATACAA 
ATTGCTTCTC TCTCTAAAGA CTCTACAGTT GAAGTAAATC CTGATGAGAA AGAAGGCTTG 
GAGCGTCGTT CTCGTGCTTT TGAACTGTTG ATTAACGCTT TTGTTCAACT ACAACACGAT 
GTAAACAGCC TGATTGATAG TAATAGGAAT CCTAGTTCTG GAGGACAGAG TAGAACAGTA 
CCCCCGGGTA TTAAGCAAAT TTTGGAAAAG AAGGAGGGTC TTTTCCGTAA GCATATGATG 
GGTAAACGTG TGAATTATGC AGCTAGATCT GTCATTTCAC CTGACCCGAA CATTGAAACT 
AATGAAATCG GTGTTCCGCC AGTCTTTGCT ACTAAACTCA CATACCCAGA ACCAGTAACT 
CTTTACAACT TTAACGAGAT GAGAAATGCG GTTATTAATG GTCCCCATAA ATGGCCTGGT 
GCTAGTCATA TTCAAAACGA AGACGGCACT TTAATTTCTT TAATGCCTTT AACAATTGAA 
CAACGCACTG CTTTAGCCAA CCAGCTTCTT ACACCTCAAA GCAATTTAAT ATCTTCCCCA 
TATTCTTATT CTCGCTTAAT AAATACTAAC AAAAAGGTTT ATCGTCATGT CAGAAATGGA 
GACATGTTGA TATTGAACCG TCAACCCACC CTTCATAAGC CTTCGATGAT GGCTCATAAA 
GCTCGTATTC TTCCAGGTGA GAAGACGATT AGAATGCATT ACGCAAATTG CAACTCTTAC 
AATGCTGATT TCGATGGCGA CGAAATGAAC ATGCATTTTC CACAAAGTAC CAATGCGCGT 
TCTGAGGCTC AATTTATTGC AAACACAGAC TCTCAATATT TGGTGCCTAC CTCTGGTGAT 
CCTTTGCGCG GTCTTATTCA AGACCATGTC GTAATGGGTG TTTGGCTTAC CTGTAAAGAT 
ACATTTTATA CTCGAGATGA ATATCAGCAA CTTCTATTTC AGGCTTTAAA GCCTGATGAA 
ACCGGTATGT ATGGGCGTAT CAAAACTCTT CCTCCTGCCA TACAACGTCC GGGTATATAC 
TGGACAGGTA AACAAATAAT ATCTTCTGTT TTGCTCAACC TAAAACCCAG TGATCGTCCT 
GGTCTTAATC TTAAAAGCAA AGCAAAGGTT CCAGGAAAAT ACTGGTCACC TGACTCAGAG 
GAGGGATCAG TATTATTTGA TGATGGTGAA CTTTTATGCG GTATTCTTGA TAAGTCCTCT 
TTTGGTGCCT CGGCTTTTGG CTTAGTTCAC TCAGTTCATG AATTGTACGG TCCTGACATC 
GCTGGTCGCT TGCTTTCTGT TTTAAGTCGT CTATTCACTG CGTATGCTCA AATGCGGGGC 
TTTACTTGCC GTATGGATGA TTTGAGACTT GATGAGCAAG GTGATAATTG GAGAAGACAA 
CTATTGGAAA ACGGAAAATC CTTTGGACTT GAAGCTGCCA GTGAATATGT TGGCCTTTCA 
ACAGATTCGC CAATTGCGCT TTTGAACGCT AACCTTGAGG AAGTTTACAG AGATGATGAG 
AAGTTACAAG GTTTAGATGC TGCTATGAAA GGTAAAATGA ACGGGCTTAC TTCCTCAATT 
ATCAACAAAT GTATCCCTGA TGGTTTACTT ACAAAATTCC CCTATAATCA TATGCAAACT 
ATGACCGTTT CTGGTGCCAA GGGTAGCAAC GTAAACGTCT CACAGATTTC TTGTCTTTTG 
GGTCAACAGG AATTAGAAGG ACGTCGTGTT CCTCTTATGG TGAGCGGAAA GTCACTTCCT 
TCCTTTGTTC CATATGAAAC TTCTGCTAAG TCTGGTGGTT TTATTGCCAG CAGATTTTTA 
ACTGGTATCG CCCCTCAGGA GTATTATTTT CACTGTATGG CTGGCCGTGA AGGCTTGATT 
GATACCGCTG TTAAAACCAG TCGGTCTGGT TATCTTCAGC GTTGTCTGAT GAAGCATCTG 
GAAGGTTTAT GTGTACAATA TGATCATACT GTTCGCGATT CCGATGGATC TATCGTCCAA 
TTCCATTACG GAGAAGATTC TTTAGATGTC ACAAAACAGA AGCACTTGAC ACAATTTGAG 
TTTTCAGCCA AAAATTATAA ATCCTTAATT CAGAAATACA AGGTCAAATC TGTCTTATCG 
GCGGTTGATT CCGAAACTGC ATCCTCTTAC GCGAAGAAAG CGTTGAAAAA GCCGTACAAA 
TATGATCCAG TATTGGACAA GTATCCCCCT AGTCGATATT TGGGAAGCGT AAGCGAGAAG 
TTTCAACGTG CTGTTGATGA ATATACCCAG AAAAATCCGG ACAAGTTGAT TGCTTCGAAG 
AAAGAGAGCA AGCTTGATGA TTCTCTTCTT AATGAATCAA AGTTCAAGGC ATTGATGCAG 
TTACGTTACC AACAGTCTCT GGTTGATCCT GGTGAAAGTG TTGGTGTATT AGCTAGTCAA 
TCAATCGGTG AGCCATCTAC TCAAATGACT TTGAACACAT TCCATTTTGC TGGATTCGGT 
GCTAAGAATG TCACATTAGG TATTCCTCGT TTACGTGAGA TTATCATGAC CGCTAGTGCA 
AATATTCAAA CTCCTACGAT GACTCTTCGT TTGAATGACG GCGTTTCCGA TAAAAGAGCT 
TCAGCTTTTT GTAAAGAAGT AAATAAATTG GTTTTATCTG AAGTTGTTCG ACAAGTCCGA 
GTAACTGAAA AAATTTCTGG TCAAGGCTCT GACGAACAAT CTAAAACTTA TGCTATTCGA 
CTTGATTTAT ATTCTCGTGA TGAATATCAG GATGAATATG GTGTTCTTCA GGAAGAGATT 
GAGTCGACCT TTAGTAATAG ATTCTTAAAA ATTCTTAACC GAATTATTAA AAGCTATTTG 
GCTAAATCTA AGCAGCGGAA GTCAGGAGGT AAAGATGATA CAGTACCTGA GGTTGGTCAG 
GCATTAAAGC CACTTGAAGA TATCGATGAA GCACCCATTG AAGGTCGTGC TCAAGAAGCT 
CTCGAAGATG AAGACAATGA TGCTACCAAT GAGAAAATGG TCTCTAGGTC GAAACAGCAT 
GCTTCTTATG AGGGGCCTGA TGAGGCAGAC AAAGTCGCCC TTCGTCAGCT AAAAGGATCA 
AATAAAGTTG AAGACGTAAA CATGGATGAA GAGGAAGATG AGGGTTTCAA GTCCGATGAA 
AGTGTCTCTG ATTTTAAAGA AAGGAAATTG TTGGAAAAGC AGAACACAGT TTCCATTTCG 
GAAAGGCGGG AACTTCAGTT AAAGACAGCA AAAGAAATAC TCAGCAATTG CAAACACCTC 
GACTTTGATT ACGTTAACGG TGAGTGGGCA ACTGTTGAAT TGGTGTTCCC AATCAATACT 
GAAAAGCTAT TAATGGTGTC ACTTGTGGAA AAGGCATGTT CCGAAACTGT CATACACGAA 
ATTCCGGGCA TTACACGCTG TTTTTCCAAA CCTCCAGACT CTGCCTTAGA CACTGTTCCG 
AAGGTTATCA CTGAAGGTGT CAATCTGAAA GCAATTTGGG AATTTTACAA TGAAATCTCC 
ATGAATGATA TTTACACAAA TGATATTGCA GCGATCCTTA GGATATACGG TGTTGAAGCA 
GCTCGTAATG CGATTGTTCA TGAGGTTTCC TCCGTTTTTG GAGTGTATGG TATTGCTGTT 
GATCCTCGTC ATCTTTCCTT AATTGCAGAT TATATGACTT TCGAGGGAGG ATACAAAGCC 
TTCAATAGAA TGGGAATAGA ATATAACACT TCTCCATTTG CGAAAATGTC TTTTGAGACC 
ACTTGTCACT TTTTGACTGA GGCCGCCCTC CGTGGTGATG TTGATGACTT ATCCAATCCT 
TCATCTCGGT TAGTCGTTGG TCGAGTCGGT AACTTTGGCA CCGGTTCTTT TGACATATTC 
ACCCCTGTAG TCGATTCTCC TGCAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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nuc1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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