ubr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr1
Entrez Gene ID	2540764
Full Name	N-end-recognizing protein Ubr1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022077.3	NP_596158.2	N-end-recognizing protein Ubr1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors show that Schizosaccharomyces pombe has the N-end rule pathway in which only Ubr11, but not Ubr1, is responsible.
Title: Essential role of Ubr11, but not Ubr1, as an N-end rule ubiquitin ligase in Schizosaccharomyces pombe.

The following ubr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100078	NM_001022077.3 Latest version!	Schizosaccharomyces pombe N-end-recognizing protein Ubr1 (ubr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr1 ( NM_001022077.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596158.2 Schizosaccharomyces pombe N-end-recognizing protein Ubr1 (ubr1), mRNA.

Price & Availability↑

CloneID	OSc100078
Clone ID Related Accession (Same CDS sequence)	NM_001022077.3
Accession Version	NM_001022077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	N-end-recognizing protein Ubr1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022077.2.

ATGTTACAAG ACGAGTCTAG CAGATCGTTG CCTAGAAGCG CATTGATTCG GAGACGGCTT 
TCATTATTTT TACAGTCTCA TGCATTGATG TATTCATTTC TATGGTCAGA ATCTGCTAAA 
AAATCCCTTC TGAATGAAGT CTTTTCTGCG CTTCTCGGAT ATGACCATAC CTTGTGGAAT 
ACATTGCTTC CAGAGAGACC AACCATCGAT GCCTCCTTTC TTCTCCGTCG TGCTCAAGGA 
CACAGTGAGG GAGATGAGTA TCGTCATGGA ACCTGTGAGT CAAAATGTGG CCATATATTT 
CGAAAGGGTG AAGTATTCTA TCGATGCAAA ACGTGTAGTG TTGATAGCAA TTCAGCGCTG 
TGCGTGAAAT GTTTTCGTGC AACTAGTCAT AAGGATCATG AAACTTCGTT TACAGTTAGT 
GCCGGTAGCG GCGGTTGTTG TGATTGTGGT AATGCAGCTG CATGGATTGG GGATGTGAGT 
TGCAAAATAC ATTCACATGA AGAAGATGCT ACGATTAGCA ACGATATGAT AGACGAAATT 
CCGGAAAAAT TAGAAAACAG TATCCAAACA ACAATTGATT GTGTTCTTGA CTTCGTACTG 
GATGTTTTTT CCTGTAGTCC TGAAAATCTC AAAAAAATGC CCACCTTGGA ATCGATTTTG 
CAGGATGAGA AAACTTCGCG TCTCAGTGAA AATAAATACG GCGATATTGA TGATTCTTGC 
AATATGTATT CGTTAGTACT GTGGAATGAT GAAAAACACT CCTTCAAGCA ATTTTATGAA 
CAAATTACTA CTGCATTAGA ATTGCCGAAT AACGTTTTTG GAAAGAAAAT GGCAAATATT 
ATTAATGACA TCGGTAGAGC TTGCATCGTT ACTGAGACTA ATATAAAAGA GCTTCTTAAA 
ATTGGTCAAA AGCTTGCCCA GATCAATCTA GCTGTTTCAA TTCGTTCTAT GCGTGACATT 
TTTAGAGAAG AATCTTGTGC TGTTTTACTA GAATGGCTTG CTGATATAGC AGGAAGCAGT 
ATTTGTGGGA AAAGAAATTA TTTTAGCTCT GTTATATGCA AAGAACTTGT TCGACCTTGG 
AATTGCGGAC TTCATAATTC TGATCTTACT TTTAGGCTCT CCCTTAGAAG TCTTGCTCTT 
CCTGAAATTG TGGCCATTGA CAGTCCTGAT ATATTTTTGA ATGAAGATCA CATTAACTCG 
TCAGGTCCAT CTGACACTTC TAGTCATATG CTAGAAACTG ACGAGTCTTC TATTCATTCT 
AGACATTGGT ATCCTTCTAA TTCTCTTCCG GATGTCCTTT CGTATGCCTC CAGAGTAAGA 
TTTGATTATT TTTTTCTTTA CGATTTAAAA CTTTGGAAGA GCTTGCGTTA CAAATTACAG 
GAGTTGTACT TGGGATACTT TATCACTCAG CCTGGATTTA AGGAAATTAT GGGAGCCAGG 
ATTGCCATTT CTTATAGAAG GTTGGCAGAA CTTTTTCTTC TGTTGGACCG TGAACCTGAA 
CACTCAGTCA TTTTTTTTTC AATGCAAATA TTTACCGTGG CAGACGTTGC CAAACTGTTG 
GTTACAGAGT ATGATTTCTT GACTACAATT AATGCAACTT TGTACACTTT TTTCACCTAT 
AAAAAACTTA ATACGCCGAA CTATGTCGAT CAGCATGCCA TGATAAGAAC AGATTCGGCT 
GCCTTTCATT CACGTAGGTA TATTCACATT TTCCATCACA TTCAATTTAT GTTAAGCATT 
CCATGTGTTG CTGAAATTGT TCGTGAAGAC CTTAAATTTT TGAAGCAATA TGCCGATTTT 
TTTAACCTGT TTCAAGGTAT GTGCCCCTAT ACACGAGCAG TATCGCAGCA TGTTGAATGG 
GAAAATGATT CATGGATGTA CGTTTTGAAC GTTAGTTTAC AAGTTGCTAA ACTCTGTAGA 
CATGTTGGAA ATGTCTTTAT GGAGCTCAAC ACGAATAAAT TAGCCAATGC TATAAACTAC 
CTAATTTCCC TAATTCTTTA TCCTAAGGCA AGAAATGAGT CTTGGACCAA TACTGAATCA 
TTGACTACAG GAATTACTGT TGATGAAAGA GGTAATTCGA AATTAATAGA GTATGATATT 
GCTCTTCAAC CGGTATCGTT CCATCATCCT TTACACTGGT TGCTAGTGTA CCTTTTATCA 
TTTTACGTTG AACGAGACAA CTATAAATTA CTATGGACTC AGCTAGATTT ACTTGCGGTT 
ACGGATCACC CACTTCGTGT ATGCGCTTGG TTAAGTCAAA TGCGCGCAAA GCTTTGGATA 
AGGAATGGTA CAACGTTACG TGATCAAGCT CACCATTATA GAAATCTAAG TTTTCATGAA 
TACACTTTTG ATTTAGACGT TTTGTTGCTT CAATTAACTT TAACATATGG CGATCCTGAT 
GCTATCTTAC CTTCTTTTAT TTCTCGATTT CAATTAGAAG ATCAAATGTA TGGTCGGTTT 
TTTGTACCTC ATAAACATTA TGATGTATCC CAAGTCACGA TAATGATGGA GGAGTTTTTG 
CTGCTTTTAA TATCCATTGT ATGTAATACT GCTGTCCTCG ACCATTGGGA TATTACAAGA 
CGAATAGAAT ATGGAATCGC TCATATCCTT TGTTTTCGTC CTTTACCTTA TTCCGAGATT 
ACCAAAAGGA CATGTGAGCA TTTATTGGAG CACAAGCAAT TTGAAAGCAC TCTCAAGAAA 
GTCGCTACTT TTCGGAATGC CGAGGGGATC AATGATAGTG GTTCCTTCAC GTTAAAAGAT 
GAATACTTTG ATTATGTAGA TCCATTCAAT ATTCATTACT CTAGAAATCA ACGTGAGGAA 
GCAGAGAATA TTTTACGTAG ACGGTATTCA AAACAGCATT CCAAACATTT GGAATCTGTA 
GTATATGAGG AATATCATCC GATATTGCAT TCGAACATTA CAATTCCCAT ATTACAAAGC 
GATTCTTTTG TTGGGATTTT ATGGCACACT ATCGTCTACG CTTATATTTA TCCATACGAT 
CAAGGAAAAC TAGAAGGCTT GGTTAATACT GCATTACATG CATGTCTCCT AGTTTTAATG 
AGTGAAAAAG GATCTGAACC TATTTTTTCC AAAAAAATAT GTGAGAATCG ATTTCCAGTT 
GTCGAGGGAT TACAGGAATA TTGCAACTCT CCGGATGTTA CGTTGTTCAG TGTTTTGTGT 
CAAATGAAAA ACCACAGAAA CTTTGTGTAT GTCAAAGAAA AGATTTCTTT GATAATGAAA 
ATCTTAAAAA GTGAAGTTCC GTTATTGTAT GAACCTGTAT ATGCCGAAAC TTTGAGTATA 
TCCTCTTCGA AAATAGTTCA GTCGTTATCG GATGCCGAAC AGCAGGAACA ACATTTAGCT 
AAGGTGCGCA TGGCAAAGGA ACGACAGGCA CGGATAATGG AACAGTTCCG AATGCAGCAA 
AATAAGTTTT TAGAAAATCA TGCTCTTTTT GAAGCATCGG ACTGTGAAAT GGACGAAGCG 
GATGAATTTT CTGTAACCAG TTCAGTTAGC ACTAAGTTAT TCTTGGACCC ACCTATTGAT 
ACTTGTCTTC TATGTCAGGA AGAACTGAAA GATAAGCGCC CCTATGGTAC TTTGGTATTT 
GTTCTTAGGA GCTCGGTACT CAGACTATTT CCTGCTGATG ATGCCAACTA CGTTTCTGAA 
GTGTTGGATA TTCCTGACAG CTTGGATCAT GAGATTCAAG AAAGACCTTT TGGATTGGCT 
GGCAAACGAA AAAAGGTATT AGATTCTACA GAAGCTTACG ATTATGATAA TTACTATTAT 
GAAAAAAAGG GCAATGAACT CCATCAGTTA AAGGACTCTT TTAATGGATT TCCCCCTGAT 
CAGCTGGATC GTGGATTGCA TGCCACTGGA TGTGGTCATT TCATGCATAT CGATTGTTTT 
AAAAATCATA TTGCTACTGT CACACTTGCA ACTCGAGCCA ATCCTTACAG AAATCACCCT 
CATAATTTAA GCATGAAAGA ATTCCTTTGC CCTCTATGCA AAGCATTATG TAATACTATT 
TTTCCAATCT TATGGCGGCC TAAGGAAGAG ATTAATTTTC AAGAAGCTGG AGTACTTACC 
GCTCCTTTGA AAAATTGGCT GGTTTCTAAA ACATTTTCAT TTAATAAAGA CTTAAATCAG 
CAACTTCTTG ACATAGAAAC CTCCCCTTCT GAGCACACAC AGTCGTACAA TTTAAATCTG 
CTTGATGTAT TACAACACAC CTTACGAGAC TCGTTGAAAG ATATATATAC CCTCAATACT 
GGTGCTGATA ATTCCAGTGA TAACGTTGAA GAAAATGCGG ATAACTTATT CCAATCGAGC 
GTATTGGATC ATGTCCATTT TAAGAGTGTT GTAAATAATG AAGTTCCTGC CGATGAACGT 
CTCGCTATTT CAGATGATAT ATTTGAGCTT TATCGGAGGC TTGACGATGT AATAGATCTT 
AATTCTTCTT TATACTCCGA TGATTTTATT CCAGTGAACG GTAAATTGCA TAATGTTGTT 
AAACTCTTTT CATACAGCCT TTGCCAAGTT GAAGCATCTA CTAGAGGACA CATAAAATGT 
TCAAGTATTC CAGCGGATAT TTGGGTGCAT AATCTTGGAA AAAATCAGCA AGTTTTTTTA 
AGGATTTTAT CTGAATCTAT CAAAACTTAT ACTTTGTTAT GTGCACATGA TTCACAGAAG 
CGTATTGGGG GTTCAATTCA AGAGTTTGAA TTTATTAGTT TCTGCCAACA AAAAAGGATA 
TTTGGGCGAC TATTGCCCTC TCTAGATTCA CCGGTTACCA AATCTATTAC TGATGATAGA 
GTTGAGCCGT TACTTGTGAA GGATACATTT CGAGAATTCG CCGAAGCCAG TGTCTCTGGT 
TTATTATCAT GTGACGAATC GTTTCACTAC TTGACGCAAT TATACTATAC TGCCGATATA 
GTTCGAAACT TATGGATTCT TTTGTCACAA AGAAACTCTT TACTGAAATG TATGGAATCA 
GTTGAATTTG AAGCCTTTGA CTATGAGCAG CTAAAGGGGT TTGAGCACTT AGTTATCCAA 
ATTTGGAAAA GTCTTCGTGT CGACGGAGCA GGATTAATCA ATTTTGATTG TTGTACGGAG 
GATGATCTAA ATAATCCTCA TCTTTTGTTT ACACTGTATA AACTATTGGA AAGGTTTTCT 
CTAATCTTCC TTCGAAAATG TGCTTTGCTT TGGTATTGCC GCTATGGAGT TTCATTTGAG 
ACGCAACCGA ATTTAAACTT TCAAAATTCA GAATTATCAC GTTTACAAAC AAAAATGCAT 
ATTCCAGGTG TTATTGAATT ATCTAATCAT CTTTGTCTTA CGGCATCTTC CACAGAATGG 
AGCCTTATAA AGCACTGGTG TAATTTCTTT ACAGAGACTG GTCCTCTATG CGATTTTCCT 
CGCGCTTATT ACCCTGGAAT TTATGAACTA GTTTCTTTAC CGTATGAATT GGATAAAGTC 
TTTGAGTTAC TATTGGCCAG GCGATGCTCG AAATGTTTAA CTGAACCTAT GGAACCAGCG 
ATATGTTTAT TTTGTGGGAA GCTCTTATGC TTCCAAAGTC ATTGTTGTTC CTTTAATGGT 
ATTGGAGAAT GTAATCTTCA TATGCAACAA TGCGCTTCTG ACATTGGAAT TTTTCTTATT 
GTCAAAAAAT GCGCTATTCT CTACCTCAAT CCACCTGTAG GAAGCTTCTC TGTAGCTCCA 
TTTTTGGATG CGTATGGAGA GACAGATTTG GGATTGCGTC GTGGCCGCTC TCAATATCTA 
TCACAAAAAC GTTATGATGA AACAGTACGA ACGATGTGGC TAAACGGATC TATTCCTTCT 
TATATTGCTC GACAACTGGA TGCGAATCCA GACACCGGAG GTTGGGAAAC ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596158.2
CDS	242..6118
Translation	MLQDESSRSLPRSALIRRRLSLFLQSHALMYSFLWSESAKKSLLNEVFSALLGYDHTLWNTLLPERPTIDASFLLRRAQGHSEGDEYRHGTCESKCGHIFRKGEVFYRCKTCSVDSNSALCVKCFRATSHKDHETSFTVSAGSGGCCDCGNAAAWIGDVSCKIHSHEEDATISNDMIDEIPEKLENSIQTTIDCVLDFVLDVFSCSPENLKKMPTLESILQDEKTSRLSENKYGDIDDSCNMYSLVLWNDEKHSFKQFYEQITTALELPNNVFGKKMANIINDIGRACIVTETNIKELLKIGQKLAQINLAVSIRSMRDIFREESCAVLLEWLADIAGSSICGKRNYFSSVICKELVRPWNCGLHNSDLTFRLSLRSLALPEIVAIDSPDIFLNEDHINSSGPSDTSSHMLETDESSIHSRHWYPSNSLPDVLSYASRVRFDYFFLYDLKLWKSLRYKLQELYLGYFITQPGFKEIMGARIAISYRRLAELFLLLDREPEHSVIFFSMQIFTVADVAKLLVTEYDFLTTINATLYTFFTYKKLNTPNYVDQHAMIRTDSAAFHSRRYIHIFHHIQFMLSIPCVAEIVREDLKFLKQYADFFNLFQGMCPYTRAVSQHVEWENDSWMYVLNVSLQVAKLCRHVGNVFMELNTNKLANAINYLISLILYPKARNESWTNTESLTTGITVDERGNSKLIEYDIALQPVSFHHPLHWLLVYLLSFYVERDNYKLLWTQLDLLAVTDHPLRVCAWLSQMRAKLWIRNGTTLRDQAHHYRNLSFHEYTFDLDVLLLQLTLTYGDPDAILPSFISRFQLEDQMYGRFFVPHKHYDVSQVTIMMEEFLLLLISIVCNTAVLDHWDITRRIEYGIAHILCFRPLPYSEITKRTCEHLLEHKQFESTLKKVATFRNAEGINDSGSFTLKDEYFDYVDPFNIHYSRNQREEAENILRRRYSKQHSKHLESVVYEEYHPILHSNITIPILQSDSFVGILWHTIVYAYIYPYDQGKLEGLVNTALHACLLVLMSEKGSEPIFSKKICENRFPVVEGLQEYCNSPDVTLFSVLCQMKNHRNFVYVKEKISLIMKILKSEVPLLYEPVYAETLSISSSKIVQSLSDAEQQEQHLAKVRMAKERQARIMEQFRMQQNKFLENHALFEASDCEMDEADEFSVTSSVSTKLFLDPPIDTCLLCQEELKDKRPYGTLVFVLRSSVLRLFPADDANYVSEVLDIPDSLDHEIQERPFGLAGKRKKVLDSTEAYDYDNYYYEKKGNELHQLKDSFNGFPPDQLDRGLHATGCGHFMHIDCFKNHIATVTLATRANPYRNHPHNLSMKEFLCPLCKALCNTIFPILWRPKEEINFQEAGVLTAPLKNWLVSKTFSFNKDLNQQLLDIETSPSEHTQSYNLNLLDVLQHTLRDSLKDIYTLNTGADNSSDNVEENADNLFQSSVLDHVHFKSVVNNEVPADERLAISDDIFELYRRLDDVIDLNSSLYSDDFIPVNGKLHNVVKLFSYSLCQVEASTRGHIKCSSIPADIWVHNLGKNQQVFLRILSESIKTYTLLCAHDSQKRIGGSIQEFEFISFCQQKRIFGRLLPSLDSPVTKSITDDRVEPLLVKDTFREFAEASVSGLLSCDESFHYLTQLYYTADIVRNLWILLSQRNSLLKCMESVEFEAFDYEQLKGFEHLVIQIWKSLRVDGAGLINFDCCTEDDLNNPHLLFTLYKLLERFSLIFLRKCALLWYCRYGVSFETQPNLNFQNSELSRLQTKMHIPGVIELSNHLCLTASSTEWSLIKHWCNFFTETGPLCDFPRAYYPGIYELVSLPYELDKVFELLLARRCSKCLTEPMEPAICLFCGKLLCFQSHCCSFNGIGECNLHMQQCASDIGIFLIVKKCAILYLNPPVGSFSVAPFLDAYGETDLGLRRGRSQYLSQKRYDETVRTMWLNGSIPSYIARQLDANPDTGGWETL

Target ORF information:

RefSeq Version	NM_001022077.3
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe N-end-recognizing protein Ubr1 (ubr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022077.3

ORF Insert Sequence:

ATGTTACAAG ACGAGTCTAG CAGATCGTTG CCTAGAAGCG CATTGATTCG GAGACGGCTT 
TCATTATTTT TACAGTCTCA TGCATTGATG TATTCATTTC TATGGTCAGA ATCTGCTAAA 
AAATCCCTTC TGAATGAAGT CTTTTCTGCG CTTCTCGGAT ATGACCATAC CTTGTGGAAT 
ACATTGCTTC CAGAGAGACC AACCATCGAT GCCTCCTTTC TTCTCCGTCG TGCTCAAGGA 
CACAGTGAGG GAGATGAGTA TCGTCATGGA ACCTGTGAGT CAAAATGTGG CCATATATTT 
CGAAAGGGTG AAGTATTCTA TCGATGCAAA ACGTGTAGTG TTGATAGCAA TTCAGCGCTG 
TGCGTGAAAT GTTTTCGTGC AACTAGTCAT AAGGATCATG AAACTTCGTT TACAGTTAGT 
GCCGGTAGCG GCGGTTGTTG TGATTGTGGT AATGCAGCTG CATGGATTGG GGATGTGAGT 
TGCAAAATAC ATTCACATGA AGAAGATGCT ACGATTAGCA ACGATATGAT AGACGAAATT 
CCGGAAAAAT TAGAAAACAG TATCCAAACA ACAATTGATT GTGTTCTTGA CTTCGTACTG 
GATGTTTTTT CCTGTAGTCC TGAAAATCTC AAAAAAATGC CCACCTTGGA ATCGATTTTG 
CAGGATGAGA AAACTTCGCG TCTCAGTGAA AATAAATACG GCGATATTGA TGATTCTTGC 
AATATGTATT CGTTAGTACT GTGGAATGAT GAAAAACACT CCTTCAAGCA ATTTTATGAA 
CAAATTACTA CTGCATTAGA ATTGCCGAAT AACGTTTTTG GAAAGAAAAT GGCAAATATT 
ATTAATGACA TCGGTAGAGC TTGCATCGTT ACTGAGACTA ATATAAAAGA GCTTCTTAAA 
ATTGGTCAAA AGCTTGCCCA GATCAATCTA GCTGTTTCAA TTCGTTCTAT GCGTGACATT 
TTTAGAGAAG AATCTTGTGC TGTTTTACTA GAATGGCTTG CTGATATAGC AGGAAGCAGT 
ATTTGTGGGA AAAGAAATTA TTTTAGCTCT GTTATATGCA AAGAACTTGT TCGACCTTGG 
AATTGCGGAC TTCATAATTC TGATCTTACT TTTAGGCTCT CCCTTAGAAG TCTTGCTCTT 
CCTGAAATTG TGGCCATTGA CAGTCCTGAT ATATTTTTGA ATGAAGATCA CATTAACTCG 
TCAGGTCCAT CTGACACTTC TAGTCATATG CTAGAAACTG ACGAGTCTTC TATTCATTCT 
AGACATTGGT ATCCTTCTAA TTCTCTTCCG GATGTCCTTT CGTATGCCTC CAGAGTAAGA 
TTTGATTATT TTTTTCTTTA CGATTTAAAA CTTTGGAAGA GCTTGCGTTA CAAATTACAG 
GAGTTGTACT TGGGATACTT TATCACTCAG CCTGGATTTA AGGAAATTAT GGGAGCCAGG 
ATTGCCATTT CTTATAGAAG GTTGGCAGAA CTTTTTCTTC TGTTGGACCG TGAACCTGAA 
CACTCAGTCA TTTTTTTTTC AATGCAAATA TTTACCGTGG CAGACGTTGC CAAACTGTTG 
GTTACAGAGT ATGATTTCTT GACTACAATT AATGCAACTT TGTACACTTT TTTCACCTAT 
AAAAAACTTA ATACGCCGAA CTATGTCGAT CAGCATGCCA TGATAAGAAC AGATTCGGCT 
GCCTTTCATT CACGTAGGTA TATTCACATT TTCCATCACA TTCAATTTAT GTTAAGCATT 
CCATGTGTTG CTGAAATTGT TCGTGAAGAC CTTAAATTTT TGAAGCAATA TGCCGATTTT 
TTTAACCTGT TTCAAGGTAT GTGCCCCTAT ACACGAGCAG TATCGCAGCA TGTTGAATGG 
GAAAATGATT CATGGATGTA CGTTTTGAAC GTTAGTTTAC AAGTTGCTAA ACTCTGTAGA 
CATGTTGGAA ATGTCTTTAT GGAGCTCAAC ACGAATAAAT TAGCCAATGC TATAAACTAC 
CTAATTTCCC TAATTCTTTA TCCTAAGGCA AGAAATGAGT CTTGGACCAA TACTGAATCA 
TTGACTACAG GAATTACTGT TGATGAAAGA GGTAATTCGA AATTAATAGA GTATGATATT 
GCTCTTCAAC CGGTATCGTT CCATCATCCT TTACACTGGT TGCTAGTGTA CCTTTTATCA 
TTTTACGTTG AACGAGACAA CTATAAATTA CTATGGACTC AGCTAGATTT ACTTGCGGTT 
ACGGATCACC CACTTCGTGT ATGCGCTTGG TTAAGTCAAA TGCGCGCAAA GCTTTGGATA 
AGGAATGGTA CAACGTTACG TGATCAAGCT CACCATTATA GAAATCTAAG TTTTCATGAA 
TACACTTTTG ATTTAGACGT TTTGTTGCTT CAATTAACTT TAACATATGG CGATCCTGAT 
GCTATCTTAC CTTCTTTTAT TTCTCGATTT CAATTAGAAG ATCAAATGTA TGGTCGGTTT 
TTTGTACCTC ATAAACATTA TGATGTATCC CAAGTCACGA TAATGATGGA GGAGTTTTTG 
CTGCTTTTAA TATCCATTGT ATGTAATACT GCTGTCCTCG ACCATTGGGA TATTACAAGA 
CGAATAGAAT ATGGAATCGC TCATATCCTT TGTTTTCGTC CTTTACCTTA TTCCGAGATT 
ACCAAAAGGA CATGTGAGCA TTTATTGGAG CACAAGCAAT TTGAAAGCAC TCTCAAGAAA 
GTCGCTACTT TTCGGAATGC CGAGGGGATC AATGATAGTG GTTCCTTCAC GTTAAAAGAT 
GAATACTTTG ATTATGTAGA TCCATTCAAT ATTCATTACT CTAGAAATCA ACGTGAGGAA 
GCAGAGAATA TTTTACGTAG ACGGTATTCA AAACAGCATT CCAAACATTT GGAATCTGTA 
GTATATGAGG AATATCATCC GATATTGCAT TCGAACATTA CAATTCCCAT ATTACAAAGC 
GATTCTTTTG TTGGGATTTT ATGGCACACT ATCGTCTACG CTTATATTTA TCCATACGAT 
CAAGGAAAAC TAGAAGGCTT GGTTAATACT GCATTACATG CATGTCTCCT AGTTTTAATG 
AGTGAAAAAG GATCTGAACC TATTTTTTCC AAAAAAATAT GTGAGAATCG ATTTCCAGTT 
GTCGAGGGAT TACAGGAATA TTGCAACTCT CCGGATGTTA CGTTGTTCAG TGTTTTGTGT 
CAAATGAAAA ACCACAGAAA CTTTGTGTAT GTCAAAGAAA AGATTTCTTT GATAATGAAA 
ATCTTAAAAA GTGAAGTTCC GTTATTGTAT GAACCTGTAT ATGCCGAAAC TTTGAGTATA 
TCCTCTTCGA AAATAGTTCA GTCGTTATCG GATGCCGAAC AGCAGGAACA ACATTTAGCT 
AAGGTGCGCA TGGCAAAGGA ACGACAGGCA CGGATAATGG AACAGTTCCG AATGCAGCAA 
AATAAGTTTT TAGAAAATCA TGCTCTTTTT GAAGCATCGG ACTGTGAAAT GGACGAAGCG 
GATGAATTTT CTGTAACCAG TTCAGTTAGC ACTAAGTTAT TCTTGGACCC ACCTATTGAT 
ACTTGTCTTC TATGTCAGGA AGAACTGAAA GATAAGCGCC CCTATGGTAC TTTGGTATTT 
GTTCTTAGGA GCTCGGTACT CAGACTATTT CCTGCTGATG ATGCCAACTA CGTTTCTGAA 
GTGTTGGATA TTCCTGACAG CTTGGATCAT GAGATTCAAG AAAGACCTTT TGGATTGGCT 
GGCAAACGAA AAAAGGTATT AGATTCTACA GAAGCTTACG ATTATGATAA TTACTATTAT 
GAAAAAAAGG GCAATGAACT CCATCAGTTA AAGGACTCTT TTAATGGATT TCCCCCTGAT 
CAGCTGGATC GTGGATTGCA TGCCACTGGA TGTGGTCATT TCATGCATAT CGATTGTTTT 
AAAAATCATA TTGCTACTGT CACACTTGCA ACTCGAGCCA ATCCTTACAG AAATCACCCT 
CATAATTTAA GCATGAAAGA ATTCCTTTGC CCTCTATGCA AAGCATTATG TAATACTATT 
TTTCCAATCT TATGGCGGCC TAAGGAAGAG ATTAATTTTC AAGAAGCTGG AGTACTTACC 
GCTCCTTTGA AAAATTGGCT GGTTTCTAAA ACATTTTCAT TTAATAAAGA CTTAAATCAG 
CAACTTCTTG ACATAGAAAC CTCCCCTTCT GAGCACACAC AGTCGTACAA TTTAAATCTG 
CTTGATGTAT TACAACACAC CTTACGAGAC TCGTTGAAAG ATATATATAC CCTCAATACT 
GGTGCTGATA ATTCCAGTGA TAACGTTGAA GAAAATGCGG ATAACTTATT CCAATCGAGC 
GTATTGGATC ATGTCCATTT TAAGAGTGTT GTAAATAATG AAGTTCCTGC CGATGAACGT 
CTCGCTATTT CAGATGATAT ATTTGAGCTT TATCGGAGGC TTGACGATGT AATAGATCTT 
AATTCTTCTT TATACTCCGA TGATTTTATT CCAGTGAACG GTAAATTGCA TAATGTTGTT 
AAACTCTTTT CATACAGCCT TTGCCAAGTT GAAGCATCTA CTAGAGGACA CATAAAATGT 
TCAAGTATTC CAGCGGATAT TTGGGTGCAT AATCTTGGAA AAAATCAGCA AGTTTTTTTA 
AGGATTTTAT CTGAATCTAT CAAAACTTAT ACTTTGTTAT GTGCACATGA TTCACAGAAG 
CGTATTGGGG GTTCAATTCA AGAGTTTGAA TTTATTAGTT TCTGCCAACA AAAAAGGATA 
TTTGGGCGAC TATTGCCCTC TCTAGATTCA CCGGTTACCA AATCTATTAC TGATGATAGA 
GTTGAGCCGT TACTTGTGAA GGATACATTT CGAGAATTCG CCGAAGCCAG TGTCTCTGGT 
TTATTATCAT GTGACGAATC GTTTCACTAC TTGACGCAAT TATACTATAC TGCCGATATA 
GTTCGAAACT TATGGATTCT TTTGTCACAA AGAAACTCTT TACTGAAATG TATGGAATCA 
GTTGAATTTG AAGCCTTTGA CTATGAGCAG CTAAAGGGGT TTGAGCACTT AGTTATCCAA 
ATTTGGAAAA GTCTTCGTGT CGACGGAGCA GGATTAATCA ATTTTGATTG TTGTACGGAG 
GATGATCTAA ATAATCCTCA TCTTTTGTTT ACACTGTATA AACTATTGGA AAGGTTTTCT 
CTAATCTTCC TTCGAAAATG TGCTTTGCTT TGGTATTGCC GCTATGGAGT TTCATTTGAG 
ACGCAACCGA ATTTAAACTT TCAAAATTCA GAATTATCAC GTTTACAAAC AAAAATGCAT 
ATTCCAGGTG TTATTGAATT ATCTAATCAT CTTTGTCTTA CGGCATCTTC CACAGAATGG 
AGCCTTATAA AGCACTGGTG TAATTTCTTT ACAGAGACTG GTCCTCTATG CGATTTTCCT 
CGCGCTTATT ACCCTGGAAT TTATGAACTA GTTTCTTTAC CGTATGAATT GGATAAAGTC 
TTTGAGTTAC TATTGGCCAG GCGATGCTCG AAATGTTTAA CTGAACCTAT GGAACCAGCG 
ATATGTTTAT TTTGTGGGAA GCTCTTATGC TTCCAAAGTC ATTGTTGTTC CTTTAATGGT 
ATTGGAGAAT GTAATCTTCA TATGCAACAA TGCGCTTCTG ACATTGGAAT TTTTCTTATT 
GTCAAAAAAT GCGCTATTCT CTACCTCAAT CCACCTGTAG GAAGCTTCTC TGTAGCTCCA 
TTTTTGGATG CGTATGGAGA GACAGATTTG GGATTGCGTC GTGGCCGCTC TCAATATCTA 
TCACAAAAAC GTTATGATGA AACAGTACGA ACGATGTGGC TAAACGGATC TATTCCTTCT 
TATATTGCTC GACAACTGGA TGCGAATCCA GACACCGGAG GTTGGGAAAC ACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ubr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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