spo5 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	spo5
Entrez Gene ID	2540637
Full Name	meiotic RNA-binding protein 1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021957.2	NP_596047.1	meiotic RNA-binding protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spo5 is exported from the nucleus with mRNAs via the Rae1-dependent pathway.
Title: Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast.

Spo5 is a novel regulator of meiosis II that controls the level of cdc13 expression and promotes de novo synthesis of Cdc13.
Title: The RNA-binding protein Spo5 promotes meiosis II by regulating cyclin Cdc13 in fission yeast.

The meiotic function of Spo5 is regulated by phosphorylation of the residues T29, T55, S59 and/or T63 and is required for the timely degradation of Spo5 during meiosis.
Title: Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe.

The following spo5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spo5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101328	NM_001021957.2 Latest version!	Schizosaccharomyces pombe meiotic RNA-binding protein 1 (spo5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spo5 ( NM_001021957.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596047.1 Schizosaccharomyces pombe meiotic RNA-binding protein 1 (spo5), mRNA.

Price & Availability↑

CloneID	OSc101328
Clone ID Related Accession (Same CDS sequence)	NM_001021957.2
Accession Version	NM_001021957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	meiotic RNA-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021957.1.

ATGAATGGAA TAATTACGCC TCAAAAACAA AAACAATTAA TGTCTTCTCC TTCTCGTGAT 
CCATTATCCA CTACCGAATT GTCTACTCCA ACAAGTCAAA CAACTGTGGA TGTCAATGAC 
ACGAAAAAAA GTGAAGGATT GGACTCTACA ATTATACTAT TAACACCAGG AACAAGCCCA 
AACGCAACGC CTGGTTCTAG CGAGTTAGGG CTTTCCAAGA AGCCAAACAG TATAAAAAAC 
AATACTTATT CTACAGCAGC ACAGGCCGCG TATTTAAATA GGCGTGCTGC TGATCAATCA 
ACCGTTATGA CAACCGATCC TATTTCATAT GCAAATAACA ATATTAATAA TGGAACACTG 
CCACAACAAA ACGCCTACTA TGCCAACTCT TATTATCCGT CATACTATGC ACAGTCACAA 
GCCATTTCCA ACAATCGACC GGGTGTCTCT GGATTCCGTC CTGCATTCAA TTCTGTAGCT 
CCTTGCTATG GTTCACAATG GCAGACTCAT CAGTCTGTTC ACCCTTATCA TGTTAACAAT 
ACTCATCAGT ATTTAAAACC ATATGTTCAG AATGTATATC CTCAAATGCC CTCTTTAAAT 
CAGCCGGGAC TTCATATTGT CAATCAACCA ACATATCTCG CGCCAGTTCC TTCAGCGACT 
GTGCCTACGA ATTCAGTTTC TCTCTCGATG CCTTCTTTTT CCCAAGGGCA AAAAAATATT 
CCCGCTGCCA TTAATCAAGA AATGAGTGTC GGTACAACAA AGGAGAATAC AAATTACCTT 
TCTCAACTGG TTGGATTGCA TCCTGCAATT CCTCCTGCCA TTCCCAGCAT GTTTCCCATG 
TCTCATGATA ATAAAAAAAG TAACATGGAA AGCACTTCTC GTACTCGGAA TGTTTACATT 
CGTGGTTTAC CTCCCAACAC TTCGGATGAA AATTTGTTGC TTTACACCAA TCGTTTTGGT 
AAAGTTAGTA GCAGCAAAGC AATAATTGAT ATGGAAACGA ATTTATGTAA AGGATATGGG 
TTTGCATGCT TTGAAGAAGA GAAATCTGCA TTGATTTGCA TTTCAGCCAT GACTTTATGT 
GGATATCAGT GTTCATTTGC AAAAGAAAGT TTCAGTGCAC GTCTTCAAAG TCTACAAGAC 
ACAGAAAGCA CCAATCTGTA TATTTCTAAT CTTCCTCTTC ATTGGAATGA AAGTGATATT 
TCCACATTGT TTAAACCCAG CAAAATCATA TCAAACCGCG TACTTCGTGA CTCTAAGGAA 
CAATCTCGAG GTGTTGGGTT TGCTCGTATG CAAGATCGTA AAACGGCTGA AGACATAATT 
AATAAATTTA ATAACTTTGT CTTGGATCCT GCTTTACCCC CATTACAAAT CCGTTTTGCT 
GATTCCACTG ATCAGAAGAA GTTTAAAGGT CAGACTCAGA AAAGAAGATT ATGGCGTGCT 
CGTGAATACA GTGTTTTAAC TAAGGGAATG ACAGCTAATA ATGCTTTTTC CAAAGTTGAG 
GAGTTCGCAA ATAACTCTAT GCCCTCTAAA GTTGGAATGT ATGTGCCATT GGAGTCGAAC 
ACAGTCTATC AACATTCACC CACTTATGAT ACTTACGGTT GGCAAAGTAT GTATCCCTCT 
TATTCCGTTT ATCCCTCAAA CTATGAAAAC AGCAGAAGTA CTACGCCATA TCATGCCCAT 
CCCGCTACTT CTGCTGCTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596047.1
CDS	58..1761
Translation	MNGIITPQKQKQLMSSPSRDPLSTTELSTPTSQTTVDVNDTKKSEGLDSTIILLTPGTSPNATPGSSELGLSKKPNSIKNNTYSTAAQAAYLNRRAADQSTVMTTDPISYANNNINNGTLPQQNAYYANSYYPSYYAQSQAISNNRPGVSGFRPAFNSVAPCYGSQWQTHQSVHPYHVNNTHQYLKPYVQNVYPQMPSLNQPGLHIVNQPTYLAPVPSATVPTNSVSLSMPSFSQGQKNIPAAINQEMSVGTTKENTNYLSQLVGLHPAIPPAIPSMFPMSHDNKKSNMESTSRTRNVYIRGLPPNTSDENLLLYTNRFGKVSSSKAIIDMETNLCKGYGFACFEEEKSALICISAMTLCGYQCSFAKESFSARLQSLQDTESTNLYISNLPLHWNESDISTLFKPSKIISNRVLRDSKEQSRGVGFARMQDRKTAEDIINKFNNFVLDPALPPLQIRFADSTDQKKFKGQTQKRRLWRAREYSVLTKGMTANNAFSKVEEFANNSMPSKVGMYVPLESNTVYQHSPTYDTYGWQSMYPSYSVYPSNYENSRSTTPYHAHPATSAAN

Target ORF information:

RefSeq Version	NM_001021957.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe meiotic RNA-binding protein 1 (spo5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021957.2

ORF Insert Sequence:

ATGAATGGAA TAATTACGCC TCAAAAACAA AAACAATTAA TGTCTTCTCC TTCTCGTGAT 
CCATTATCCA CTACCGAATT GTCTACTCCA ACAAGTCAAA CAACTGTGGA TGTCAATGAC 
ACGAAAAAAA GTGAAGGATT GGACTCTACA ATTATACTAT TAACACCAGG AACAAGCCCA 
AACGCAACGC CTGGTTCTAG CGAGTTAGGG CTTTCCAAGA AGCCAAACAG TATAAAAAAC 
AATACTTATT CTACAGCAGC ACAGGCCGCG TATTTAAATA GGCGTGCTGC TGATCAATCA 
ACCGTTATGA CAACCGATCC TATTTCATAT GCAAATAACA ATATTAATAA TGGAACACTG 
CCACAACAAA ACGCCTACTA TGCCAACTCT TATTATCCGT CATACTATGC ACAGTCACAA 
GCCATTTCCA ACAATCGACC GGGTGTCTCT GGATTCCGTC CTGCATTCAA TTCTGTAGCT 
CCTTGCTATG GTTCACAATG GCAGACTCAT CAGTCTGTTC ACCCTTATCA TGTTAACAAT 
ACTCATCAGT ATTTAAAACC ATATGTTCAG AATGTATATC CTCAAATGCC CTCTTTAAAT 
CAGCCGGGAC TTCATATTGT CAATCAACCA ACATATCTCG CGCCAGTTCC TTCAGCGACT 
GTGCCTACGA ATTCAGTTTC TCTCTCGATG CCTTCTTTTT CCCAAGGGCA AAAAAATATT 
CCCGCTGCCA TTAATCAAGA AATGAGTGTC GGTACAACAA AGGAGAATAC AAATTACCTT 
TCTCAACTGG TTGGATTGCA TCCTGCAATT CCTCCTGCCA TTCCCAGCAT GTTTCCCATG 
TCTCATGATA ATAAAAAAAG TAACATGGAA AGCACTTCTC GTACTCGGAA TGTTTACATT 
CGTGGTTTAC CTCCCAACAC TTCGGATGAA AATTTGTTGC TTTACACCAA TCGTTTTGGT 
AAAGTTAGTA GCAGCAAAGC AATAATTGAT ATGGAAACGA ATTTATGTAA AGGATATGGG 
TTTGCATGCT TTGAAGAAGA GAAATCTGCA TTGATTTGCA TTTCAGCCAT GACTTTATGT 
GGATATCAGT GTTCATTTGC AAAAGAAAGT TTCAGTGCAC GTCTTCAAAG TCTACAAGAC 
ACAGAAAGCA CCAATCTGTA TATTTCTAAT CTTCCTCTTC ATTGGAATGA AAGTGATATT 
TCCACATTGT TTAAACCCAG CAAAATCATA TCAAACCGCG TACTTCGTGA CTCTAAGGAA 
CAATCTCGAG GTGTTGGGTT TGCTCGTATG CAAGATCGTA AAACGGCTGA AGACATAATT 
AATAAATTTA ATAACTTTGT CTTGGATCCT GCTTTACCCC CATTACAAAT CCGTTTTGCT 
GATTCCACTG ATCAGAAGAA GTTTAAAGGT CAGACTCAGA AAAGAAGATT ATGGCGTGCT 
CGTGAATACA GTGTTTTAAC TAAGGGAATG ACAGCTAATA ATGCTTTTTC CAAAGTTGAG 
GAGTTCGCAA ATAACTCTAT GCCCTCTAAA GTTGGAATGT ATGTGCCATT GGAGTCGAAC 
ACAGTCTATC AACATTCACC CACTTATGAT ACTTACGGTT GGCAAAGTAT GTATCCCTCT 
TATTCCGTTT ATCCCTCAAA CTATGAAAAC AGCAGAAGTA CTACGCCATA TCATGCCCAT 
CCCGCTACTT CTGCTGCTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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spo5 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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