SPBC25H2.15 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC25H2.15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC25H2.15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100231 NM_001022272.1
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Schizosaccharomyces pombe 20S rRNA accumulation protein 4 (SPBC25H2.15), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100231
    Clone ID Related Accession (Same CDS sequence) NM_001022272.1
    Accession Version NM_001022272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product 20S rRNA accumulation protein 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCTCAAA AATGGGTTCA GACTCAGGCT TGGTTAGGTT TTCCAGACGT CCCTATTTCC 
    CAAGAAGATA AGCCAGATGA ATACTCCACA TTTCTAGGAG GGTTTCCAGT ATTTTTTGAC
    GGATGTTCAC TGAATCCCAA TATAATTAAA TGTGGAAATT GCAAAAATTT ATGTCGGTTG
    CTTCTTCAAT GCTATGCTCC GTTGGAAGGT GATAATCTCA AAGAACGAGC TTTGTATGTC
    TGGGGTTGTC ATAATCCTTC GTGTCGACGT GTTCCAAATT CAATAGTTTG TGTCAGAGGA
    GTGAGGCTTC CCCTAAAAAG TGACATTGAG GCTGTCAAAT CACCAAAAGC TATATCTCAC
    CTTGAAGAAA AAAAATCTTC ACCGAAGGAA AAAAAAGTGA ACCCGTTTGC GATTACTTCT
    GAATCTAGTA GAGGACTGAA TCCCTTTTCA GATGCTACAT CCGCAAACAA TCCATTTTCT
    CTATCCACGG ATGTCAACCC CAGTAAACCG AGTAGCAATG TCTTCAGCAA ACCCTCTTTT
    GCTGCTAAAG CTCAACAAAG CATTACCGAT CAGCAGAAAA CCCAGGCAAA AACTAAAACA
    AAACATATAG CTTTAACAAC CTCTGGTATG TCTGTACATC CACCAGTCAC CGAGACTTAC
    ACCTATCCCG TTACTGAAGC ATTTCAGGGG ATGTTTTTGG GATTGGATTT AGAGTATGTA
    CCACAGAATA AAGTGAACTC TAAAAAAGAT GATTTTAGTA CTTTTAAAAA TTACACCCCA
    TACTTGAATG ACAGCAGTGA AGCTTGGGAG AAAGAAAGTT ATGAGAAATC CCCTAGTGTG
    TATGAAAAAA CGTTTAGACT ATTCAGCGAA AAGATATCCC ATAACCCAAC TCAATGTCTA
    CGTTATGAAC GAGGTGGTAC ACCACTGCTT GCATCTGGTA GAGATAAGCT CGGTCAGCAA
    CTGAAGTCTG TTACTAATTT TGGAAAAAGC CCGGTGCCGC TGTGCCCTCT TTGCAAATCG
    CCTCGTTTAT TCGAAATGCA GCTTATGCCA CATGCGATAT CCATTCTCAA TGACGAAATA
    GCAGAATGGT CTACTATACT GGTCGCTACA TGTTCGATGG ATTGCAATCC TCCGATCAAC
    AAAGATAGAG TTGGTTATGC AGTAGAATGG GTTGGAATTC AGTGGGATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596352.1
    CDS1..1191
    Translation

    Target ORF information:

    RefSeq Version NM_001022272.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe 20S rRNA accumulation protein 4 (SPBC25H2.15), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCTCAAA AATGGGTTCA GACTCAGGCT TGGTTAGGTT TTCCAGACGT CCCTATTTCC 
    CAAGAAGATA AGCCAGATGA ATACTCCACA TTTCTAGGAG GGTTTCCAGT ATTTTTTGAC
    GGATGTTCAC TGAATCCCAA TATAATTAAA TGTGGAAATT GCAAAAATTT ATGTCGGTTG
    CTTCTTCAAT GCTATGCTCC GTTGGAAGGT GATAATCTCA AAGAACGAGC TTTGTATGTC
    TGGGGTTGTC ATAATCCTTC GTGTCGACGT GTTCCAAATT CAATAGTTTG TGTCAGAGGA
    GTGAGGCTTC CCCTAAAAAG TGACATTGAG GCTGTCAAAT CACCAAAAGC TATATCTCAC
    CTTGAAGAAA AAAAATCTTC ACCGAAGGAA AAAAAAGTGA ACCCGTTTGC GATTACTTCT
    GAATCTAGTA GAGGACTGAA TCCCTTTTCA GATGCTACAT CCGCAAACAA TCCATTTTCT
    CTATCCACGG ATGTCAACCC CAGTAAACCG AGTAGCAATG TCTTCAGCAA ACCCTCTTTT
    GCTGCTAAAG CTCAACAAAG CATTACCGAT CAGCAGAAAA CCCAGGCAAA AACTAAAACA
    AAACATATAG CTTTAACAAC CTCTGGTATG TCTGTACATC CACCAGTCAC CGAGACTTAC
    ACCTATCCCG TTACTGAAGC ATTTCAGGGG ATGTTTTTGG GATTGGATTT AGAGTATGTA
    CCACAGAATA AAGTGAACTC TAAAAAAGAT GATTTTAGTA CTTTTAAAAA TTACACCCCA
    TACTTGAATG ACAGCAGTGA AGCTTGGGAG AAAGAAAGTT ATGAGAAATC CCCTAGTGTG
    TATGAAAAAA CGTTTAGACT ATTCAGCGAA AAGATATCCC ATAACCCAAC TCAATGTCTA
    CGTTATGAAC GAGGTGGTAC ACCACTGCTT GCATCTGGTA GAGATAAGCT CGGTCAGCAA
    CTGAAGTCTG TTACTAATTT TGGAAAAAGC CCGGTGCCGC TGTGCCCTCT TTGCAAATCG
    CCTCGTTTAT TCGAAATGCA GCTTATGCCA CATGCGATAT CCATTCTCAA TGACGAAATA
    GCAGAATGGT CTACTATACT GGTCGCTACA TGTTCGATGG ATTGCAATCC TCCGATCAAC
    AAAGATAGAG TTGGTTATGC AGTAGAATGG GTTGGAATTC AGTGGGATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L