hip1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	hip1
Entrez Gene ID	2540219
Full Name	hira protein, histone chaperone Hip1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022496.2	NP_596575.1	histone chaperone Hip1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	HIR1	NP_009545.2
Eremothecium gossypii	AGOS_AGR168W	NP_986834.2
Schizosaccharomyces pombe (fission yeast)	hip1	NP_596575.1
Neurospora crassa	NCU04035	XP_957650.2
Kluyveromyces lactis	KLLA0A04928g	XP_451216.1
Magnaporthe oryzae (rice blast fungus)	MGG_00590	XP_003718450.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HIRA protein plays roles in the maintenance of both euchromatic and heterochromatic regions of the genome.
Title: The impact of the HIRA histone chaperone upon global nucleosome architecture.

Studies indicate that the fission yeast possesses two HIRA proteins Slm9 and Hip1.
Title: HIRA, a conserved histone chaperone, plays an essential role in low-dose stress response via transcriptional stimulation in fission yeast.

HIRA histone chaperone is required to maintain the protective functions of chromatin.
Title: The fission yeast HIRA histone chaperone is required for promoter silencing and the suppression of cryptic antisense transcripts.

crystallographic analysis of the apo-form of fission yeast Asf1/Cia1 (SpAsf1N; residues 1-161) as well as its complexes with the B-domain of the fission yeast HIRA orthologue Hip1 (Hip1B) and the C-terminal region of the Cac2 subunit of CAF-1 (Cac2C)
Title: Crystal structures of fission yeast histone chaperone Asf1 complexed with the Hip1 B-domain or the Cac2 C terminus.

The following hip1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hip1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100263	NM_001022496.2 Latest version!	Schizosaccharomyces pombe histone chaperone Hip1 (hip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hip1 ( NM_001022496.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596575.1 Schizosaccharomyces pombe histone chaperone Hip1 (hip1), mRNA.

Price & Availability↑

CloneID	OSc100263
Clone ID Related Accession (Same CDS sequence)	NM_001022496.2
Accession Version	NM_001022496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	histone chaperone Hip1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022496.1.

ATGAAAATTA AAAAAATTCC ATGGTTAGGA CACTTTGACG ATCGAGGGCA TCGGCTATCT 
ATATTTTCTA TTCACATACA TCCAGATGGA TCACGGATAG CAACTGGAGG ACTGGATGGT 
ACAATTCGAA TTTGGTCTAC TGAAGCTATC AACCGTGAAA ATGAGAACGA AAATGAAAAT 
GAAGATCTTC CTAAACAGCT GTGTTGTATG AGTACTCATA CTGGTACAGT TACTTCAGTA 
CGTTTTAGCC CTAATGGTCA ATACCTTGCT TCAGGTAGTG ATGATCGAGT AGTTATTATA 
TGGCACAAGG AGGAAGCAAT TCCTGGTTTA GGTTCTACGT TTGGATCCGG TGAAAAACAC 
ACTGAGAATT GGCGTAGTTA CCGACGTTTA CTGGGCCACG ATAATGATAT CCAGGATTTA 
TGTTGGAGTT ATGATAGCCA ACTTGTAGTG AGTGTCGGAT TAGATAGTTC GATTATTGTT 
TGGAACGGTA CTACTTTTGA ACGACTAAAG CGAATTGAAG CTCATCAAAG TCACGTGAAA 
GGTATAACTT TTGACCCCGC GGGTAAGTAC TTTGCCACTG AAAGCGATGA TAGAACTATA 
AAGGTCTGGA GAGTAAGTGA CTTTTCAATT GAGAAGACCA TTACAGGTCC CTTTAACAAT 
TCACCTTTAT CAACCTATTT TCGAAGGCCG TCTTGGTCAC CGGATGGAAA ACATATTGCT 
GCTCCAAATG CGATGAATGG TCCAGTGTCT TGTGTTTCGA TAATAGAGCG TGGAACATGG 
ACCAGTGAAA TTAATCTTAT CGGACATGAA GGACCTGTTG AGGTTACCGC TTTTAATCCT 
AAGTTATTCA GAGATAAAAA TGATAAACTG GTTTGTATAT TGGCTTGTGG TGGTCAAGAT 
CGATCTTTAA GTATCTGGTC AAGCGCTTTA CCTCGACCTC TTTTGTCTTG TCAAAATGTT 
TTCCAAAAAA GTATTGGTGA TGTTTGTTGG TCACCAGACG GATTGAGTTT GTTTTTATGC 
TCGTACGATG GAAATGTCTT AGTATGTACT TTTGAAAAAG AAGAGTTTGG TGACATGGTT 
AGCGATGAAG AGATATCAAA AGCGTTGGCT AAATATGGTC ATGGTCGTCA CGGTATAGTG 
CTTCCTGAAT CTGCAAAGCA ACTCGAGCTT GAGGAAACCG CCTATGCTAT CTTAAAAAAA 
CCGTCTTCTC TTTCTACAAC TGACCCAACA CTAGTCCCCC AAAGCTCTAG CACTCCAAAA 
AGTGCTCAGA AGACACCTCA AAAGTTACCC GCTTTTTTAC CAAATAGATT GACGGCTGAA 
ACTGTTGACA CAAACAAGTT AACAGCTTCT AAAGAACAAA TCGCATCACC TAAAAGACCT 
GGTCCGAGTG ATAATGGTAA CGAAATACCG ACTAAATTTG TTCAGAAAGT TACTATCACG 
AAAGAAGGTA AAAAGAGAGT TGCTCCACAG CTATTGACCA CTCTTAGTGC AACTCCAAGC 
ACTTCTCGGC TAGCTTCTAC TCAGTTACAG CATACTGGAT CATCCCAACT TCCTCCTCAA 
CAGTTTTCAC AACCTATCAA TTCATTACCT AAAGGTGGGG TTCCTATACT AATCGTTGGT 
AACAAAACAA AAGTCAACCA TGAAAACGAT GAAAGTGATC AGGCTTTACA GGAAGAGAAG 
ATAGAAGAAG GTTTATTAAA GAATTACTAT TCTTCTCTAA TTGACAGTAG TACTTCGATA 
TCTAACATAA ATTTTGAAGC TCCTCGATAT AAAACTAATA TAGTTCATTC TCTTAATAAT 
GAGCAAAAAT ACGTTTTGGA GGTGAAAAAT GGAACAAGTG AAAAAAATCC AACTAGAATA 
GTTGCCTTGG AAAATGGAAA CACTAAATGG ATGGATTACC TTCCAAGACC GGTTATACTG 
GTTACTGGGA GTATTCATTT TTGGTCAATT GCGTGCGATG ATGGATCTCT ACATTTGTAT 
TCATTGACAG GTTCAAGACT GCTACCGCCA ATAATGATTG AATCTAAGGC TTCATTTTTG 
CATTGCAATA ACGCGTATTT GCTTTGCATT TCTTCAAGCG GAATGGTATA TGCTTGGAAT 
GTGGTGAACA AGACTGCTCT ATTTACTGCC AATTCATTAG CTCCAATCCT AAGTAGAGTT 
AGCAACAATG TTACCATTGA AAACAACTCG ACTGACATTC CACACGTAGT TATTGCCAGC 
ATCAGCAAGG AAGGGGTCCC CAGTGTTACG CTTTCTACCG GCGAAACGTA CGTTTATTCG 
TCTACAATGC TTTGTTGGCA ACGAATTACT GAACCGTGGT GGGCTATAGG AAGTCGGGAG 
TGGGATTCAA GTGGATTACT GCAAAGCAAT ACTCAAACCG AAAGTCAGCC GCTCAAAATA 
TATGAACACC GAACTAATAA TGTTTTAATG GATTCTGGAA GAGGAAAACT ATTACAAAAA 
ATGGTAGCTG ATGCAATCAC TGAAGAAGGT TATGATGATT TTGAGACCAT AGTTACTATT 
AATCATTTAG AAAACAAAAT CGCTTCTGCT CGATTACTTA AGTTGGATGA TGAGTTTTTA 
GTAACAAGCG AAGTTTATGT CAGACTATTA ATGCATCATG GTCTTTGGCA AAAATTGGAG 
GAATTTTTAG GAGAGCTACG AACTCAAACA AAATGTTCAA TTAAATTATC AGGAAGAGAA 
GTTGTTGCAA AAATGTTAGT GGTTTTACGA CAAGCAGTAC AAACTGATAA TGAATTTGAT 
CGTGCAAACA AATTAATAGA AAAATACGCA TCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596575.1
CDS	150..2948
Translation	MKIKKIPWLGHFDDRGHRLSIFSIHIHPDGSRIATGGLDGTIRIWSTEAINRENENENENEDLPKQLCCMSTHTGTVTSVRFSPNGQYLASGSDDRVVIIWHKEEAIPGLGSTFGSGEKHTENWRSYRRLLGHDNDIQDLCWSYDSQLVVSVGLDSSIIVWNGTTFERLKRIEAHQSHVKGITFDPAGKYFATESDDRTIKVWRVSDFSIEKTITGPFNNSPLSTYFRRPSWSPDGKHIAAPNAMNGPVSCVSIIERGTWTSEINLIGHEGPVEVTAFNPKLFRDKNDKLVCILACGGQDRSLSIWSSALPRPLLSCQNVFQKSIGDVCWSPDGLSLFLCSYDGNVLVCTFEKEEFGDMVSDEEISKALAKYGHGRHGIVLPESAKQLELEETAYAILKKPSSLSTTDPTLVPQSSSTPKSAQKTPQKLPAFLPNRLTAETVDTNKLTASKEQIASPKRPGPSDNGNEIPTKFVQKVTITKEGKKRVAPQLLTTLSATPSTSRLASTQLQHTGSSQLPPQQFSQPINSLPKGGVPILIVGNKTKVNHENDESDQALQEEKIEEGLLKNYYSSLIDSSTSISNINFEAPRYKTNIVHSLNNEQKYVLEVKNGTSEKNPTRIVALENGNTKWMDYLPRPVILVTGSIHFWSIACDDGSLHLYSLTGSRLLPPIMIESKASFLHCNNAYLLCISSSGMVYAWNVVNKTALFTANSLAPILSRVSNNVTIENNSTDIPHVVIASISKEGVPSVTLSTGETYVYSSTMLCWQRITEPWWAIGSREWDSSGLLQSNTQTESQPLKIYEHRTNNVLMDSGRGKLLQKMVADAITEEGYDDFETIVTINHLENKIASARLLKLDDEFLVTSEVYVRLLMHHGLWQKLEEFLGELRTQTKCSIKLSGREVVAKMLVVLRQAVQTDNEFDRANKLIEKYAST

Target ORF information:

RefSeq Version	NM_001022496.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe histone chaperone Hip1 (hip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022496.2

ORF Insert Sequence:

ATGAAAATTA AAAAAATTCC ATGGTTAGGA CACTTTGACG ATCGAGGGCA TCGGCTATCT 
ATATTTTCTA TTCACATACA TCCAGATGGA TCACGGATAG CAACTGGAGG ACTGGATGGT 
ACAATTCGAA TTTGGTCTAC TGAAGCTATC AACCGTGAAA ATGAGAACGA AAATGAAAAT 
GAAGATCTTC CTAAACAGCT GTGTTGTATG AGTACTCATA CTGGTACAGT TACTTCAGTA 
CGTTTTAGCC CTAATGGTCA ATACCTTGCT TCAGGTAGTG ATGATCGAGT AGTTATTATA 
TGGCACAAGG AGGAAGCAAT TCCTGGTTTA GGTTCTACGT TTGGATCCGG TGAAAAACAC 
ACTGAGAATT GGCGTAGTTA CCGACGTTTA CTGGGCCACG ATAATGATAT CCAGGATTTA 
TGTTGGAGTT ATGATAGCCA ACTTGTAGTG AGTGTCGGAT TAGATAGTTC GATTATTGTT 
TGGAACGGTA CTACTTTTGA ACGACTAAAG CGAATTGAAG CTCATCAAAG TCACGTGAAA 
GGTATAACTT TTGACCCCGC GGGTAAGTAC TTTGCCACTG AAAGCGATGA TAGAACTATA 
AAGGTCTGGA GAGTAAGTGA CTTTTCAATT GAGAAGACCA TTACAGGTCC CTTTAACAAT 
TCACCTTTAT CAACCTATTT TCGAAGGCCG TCTTGGTCAC CGGATGGAAA ACATATTGCT 
GCTCCAAATG CGATGAATGG TCCAGTGTCT TGTGTTTCGA TAATAGAGCG TGGAACATGG 
ACCAGTGAAA TTAATCTTAT CGGACATGAA GGACCTGTTG AGGTTACCGC TTTTAATCCT 
AAGTTATTCA GAGATAAAAA TGATAAACTG GTTTGTATAT TGGCTTGTGG TGGTCAAGAT 
CGATCTTTAA GTATCTGGTC AAGCGCTTTA CCTCGACCTC TTTTGTCTTG TCAAAATGTT 
TTCCAAAAAA GTATTGGTGA TGTTTGTTGG TCACCAGACG GATTGAGTTT GTTTTTATGC 
TCGTACGATG GAAATGTCTT AGTATGTACT TTTGAAAAAG AAGAGTTTGG TGACATGGTT 
AGCGATGAAG AGATATCAAA AGCGTTGGCT AAATATGGTC ATGGTCGTCA CGGTATAGTG 
CTTCCTGAAT CTGCAAAGCA ACTCGAGCTT GAGGAAACCG CCTATGCTAT CTTAAAAAAA 
CCGTCTTCTC TTTCTACAAC TGACCCAACA CTAGTCCCCC AAAGCTCTAG CACTCCAAAA 
AGTGCTCAGA AGACACCTCA AAAGTTACCC GCTTTTTTAC CAAATAGATT GACGGCTGAA 
ACTGTTGACA CAAACAAGTT AACAGCTTCT AAAGAACAAA TCGCATCACC TAAAAGACCT 
GGTCCGAGTG ATAATGGTAA CGAAATACCG ACTAAATTTG TTCAGAAAGT TACTATCACG 
AAAGAAGGTA AAAAGAGAGT TGCTCCACAG CTATTGACCA CTCTTAGTGC AACTCCAAGC 
ACTTCTCGGC TAGCTTCTAC TCAGTTACAG CATACTGGAT CATCCCAACT TCCTCCTCAA 
CAGTTTTCAC AACCTATCAA TTCATTACCT AAAGGTGGGG TTCCTATACT AATCGTTGGT 
AACAAAACAA AAGTCAACCA TGAAAACGAT GAAAGTGATC AGGCTTTACA GGAAGAGAAG 
ATAGAAGAAG GTTTATTAAA GAATTACTAT TCTTCTCTAA TTGACAGTAG TACTTCGATA 
TCTAACATAA ATTTTGAAGC TCCTCGATAT AAAACTAATA TAGTTCATTC TCTTAATAAT 
GAGCAAAAAT ACGTTTTGGA GGTGAAAAAT GGAACAAGTG AAAAAAATCC AACTAGAATA 
GTTGCCTTGG AAAATGGAAA CACTAAATGG ATGGATTACC TTCCAAGACC GGTTATACTG 
GTTACTGGGA GTATTCATTT TTGGTCAATT GCGTGCGATG ATGGATCTCT ACATTTGTAT 
TCATTGACAG GTTCAAGACT GCTACCGCCA ATAATGATTG AATCTAAGGC TTCATTTTTG 
CATTGCAATA ACGCGTATTT GCTTTGCATT TCTTCAAGCG GAATGGTATA TGCTTGGAAT 
GTGGTGAACA AGACTGCTCT ATTTACTGCC AATTCATTAG CTCCAATCCT AAGTAGAGTT 
AGCAACAATG TTACCATTGA AAACAACTCG ACTGACATTC CACACGTAGT TATTGCCAGC 
ATCAGCAAGG AAGGGGTCCC CAGTGTTACG CTTTCTACCG GCGAAACGTA CGTTTATTCG 
TCTACAATGC TTTGTTGGCA ACGAATTACT GAACCGTGGT GGGCTATAGG AAGTCGGGAG 
TGGGATTCAA GTGGATTACT GCAAAGCAAT ACTCAAACCG AAAGTCAGCC GCTCAAAATA 
TATGAACACC GAACTAATAA TGTTTTAATG GATTCTGGAA GAGGAAAACT ATTACAAAAA 
ATGGTAGCTG ATGCAATCAC TGAAGAAGGT TATGATGATT TTGAGACCAT AGTTACTATT 
AATCATTTAG AAAACAAAAT CGCTTCTGCT CGATTACTTA AGTTGGATGA TGAGTTTTTA 
GTAACAAGCG AAGTTTATGT CAGACTATTA ATGCATCATG GTCTTTGGCA AAAATTGGAG 
GAATTTTTAG GAGAGCTACG AACTCAAACA AAATGTTCAA TTAAATTATC AGGAAGAGAA 
GTTGTTGCAA AAATGTTAGT GGTTTTACGA CAAGCAGTAC AAACTGATAA TGAATTTGAT 
CGTGCAAACA AATTAATAGA AAAATACGCA TCAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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hip1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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