ORF » Species Summary » Schizosaccharomyces pombe » tim54 cDNA ORF clone

tim54 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following tim54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tim54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101203 NM_001022620.2
Latest version!		 Schizosaccharomyces pombe putative TIM22 inner membrane protein import complex subunit Tim54 (tim54), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    tim54 ( NM_001022620.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596696.1 Schizosaccharomyces pombe putative TIM22 inner membrane protein import complex subunit Tim54 (tim54), mRNA.
    Price & Availability↑
    CloneID OSc101203
    Clone ID Related Accession (Same CDS sequence) NM_001022620.2
    Accession Version NM_001022620.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative TIM22 inner membrane protein import complex subunit Tim54
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022620.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTAAAAA CAATAAAGTC TTATATGCCT GGTCGAAATA TGTCCATATT TTTGGGATTC 
    GTGGCAGGCA TATCTGGAGC AATTTATTAT GATCGGCGTC AGAAAAATTT AATAATTGAA 
    AAGTATTGCT CTCAAGTACG CCATTTGGCT GATCAACCGA TGGCACCTCT TGAGTTACCG 
    AGAAAATTGA AGGTATATCT TCATGGACCT CCAGGGGATG GAATATACGT TGCACGGGAA 
    GAATTTGAAG AATACATTAG ACCTATATTT AATGCTGCAG CCATTGAATT TGAAACCGTG 
    GAGTCCAAAG GTGAAGGGAA TTTATTGGAA CAAGTAGCGA GGACGGTTTA CAACAAGAGG 
    CATAACATTT CTGAAGTTTC TGAACCGGAA AAAAACCTCT TATCAGTTTT AAAACCAAGT 
    GTTGATCCTC CTGCTATTGT TTTATTAGGA AGGCATGCCC TCAAAGAGTT CCTGTACGGC 
    GTTCGATATG GATTTTCCGA CGATATTATG AAAAGAAAAC TTGAAACAGA AAAATTGGAA 
    GCCAATAATA AAGAGGAGAA AGAAGAGAAA GAAGGAAAAG ATGACAAAGA TGACAAAGAG 
    GATAGTAATG ATACAAAGAA CGATAAAAAA ATTAGCAAAA ACGAAGTAGA CTCTTCTCTG 
    ATAGAAGCAA GTCCGTTGAC TGGTCAAGTT CCACCAAAAT TTTTGGATAC TATTGCTATT 
    TTTCCCTTAC CAAATCTTTT AGGGTTTTCT AACACTCCTA AGCGACTTTC AAGATTTTTT 
    AAAAGAAGAG AGCTAGCTGA TGAATTAGGC GCAATTGCCG TAAACGTTGC ATTAAGCAGG 
    GATGTAACTA AGTTTCCAAA ACAAGACGGC ACTTTACTTC TAGCTGAAGA GGAAACAGAT 
    TGGCCGAAGC AATTTTTTAC AAGATCTGAT TTGGAAAACC GCATTTGGAC AGCTCCCTTT 
    CTACAGGACT CTGATGAGAT ACGTTTTTTT GAAAACATTG ATATCTTTGA TTCAACGAAA 
    GCCAAACAAG ATAAGTATGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596696.1
    CDS217..1260
    Translation

    Target ORF information:

    RefSeq Version NM_001022620.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative TIM22 inner membrane protein import complex subunit Tim54 (tim54), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022620.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTAAAAA CAATAAAGTC TTATATGCCT GGTCGAAATA TGTCCATATT TTTGGGATTC 
    GTGGCAGGCA TATCTGGAGC AATTTATTAT GATCGGCGTC AGAAAAATTT AATAATTGAA 
    AAGTATTGCT CTCAAGTACG CCATTTGGCT GATCAACCGA TGGCACCTCT TGAGTTACCG 
    AGAAAATTGA AGGTATATCT TCATGGACCT CCAGGGGATG GAATATACGT TGCACGGGAA 
    GAATTTGAAG AATACATTAG ACCTATATTT AATGCTGCAG CCATTGAATT TGAAACCGTG 
    GAGTCCAAAG GTGAAGGGAA TTTATTGGAA CAAGTAGCGA GGACGGTTTA CAACAAGAGG 
    CATAACATTT CTGAAGTTTC TGAACCGGAA AAAAACCTCT TATCAGTTTT AAAACCAAGT 
    GTTGATCCTC CTGCTATTGT TTTATTAGGA AGGCATGCCC TCAAAGAGTT CCTGTACGGC 
    GTTCGATATG GATTTTCCGA CGATATTATG AAAAGAAAAC TTGAAACAGA AAAATTGGAA 
    GCCAATAATA AAGAGGAGAA AGAAGAGAAA GAAGGAAAAG ATGACAAAGA TGACAAAGAG 
    GATAGTAATG ATACAAAGAA CGATAAAAAA ATTAGCAAAA ACGAAGTAGA CTCTTCTCTG 
    ATAGAAGCAA GTCCGTTGAC TGGTCAAGTT CCACCAAAAT TTTTGGATAC TATTGCTATT 
    TTTCCCTTAC CAAATCTTTT AGGGTTTTCT AACACTCCTA AGCGACTTTC AAGATTTTTT 
    AAAAGAAGAG AGCTAGCTGA TGAATTAGGC GCAATTGCCG TAAACGTTGC ATTAAGCAGG 
    GATGTAACTA AGTTTCCAAA ACAAGACGGC ACTTTACTTC TAGCTGAAGA GGAAACAGAT 
    TGGCCGAAGC AATTTTTTAC AAGATCTGAT TTGGAAAACC GCATTTGGAC AGCTCCCTTT 
    CTACAGGACT CTGATGAGAT ACGTTTTTTT GAAAACATTG ATATCTTTGA TTCAACGAAA 
    GCCAAACAAG ATAAGTATGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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