ppk19 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppk19
Entrez Gene ID	2539996
Full Name	serine/threonine protein kinase Ppk19
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021195.2	NP_595288.1	serine/threonine protein kinase Ppk19

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PIK3R4	XP_004939498.1
Danio rerio (zebrafish)	pik3r4	XP_001922676.1
Oryza sativa (rice)	Os02g0796700	NP_001048391.1
Canis lupus familiaris (dog)	PIK3R4	XP_534260.1
Bos taurus (cattle)	PIK3R4	NP_001093784.1
Eremothecium gossypii	AGOS_ADL316C	NP_983780.2
Magnaporthe oryzae (rice blast fungus)	MGG_06100	XP_003711966.1
Neurospora crassa	NCU06626	XP_962368.2
	PIK3R4	XP_002802990.1
Mus musculus (house mouse)	Pik3r4	NP_001074778.1
Rattus norvegicus (Norway rat)	Pik3r4	NP_001102247.1
Saccharomyces cerevisiae (baker's yeast)	VPS15	NP_009655.2
Schizosaccharomyces pombe (fission yeast)	ppk19	NP_595288.1
Xenopus tropicalis (tropical clawed frog)	pik3r4	NP_001107701.1
Pan troglodytes (chimpanzee)	PIK3R4	XP_516746.2
Drosophila melanogaster (fruit fly)	ird1	NP_649868.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000602	XP_310476.5
Arabidopsis thaliana (thale cress)	AT4G29380	NP_194667.1
Homo sapiens (human)	PIK3R4	NP_055417.1
Kluyveromyces lactis	KLLA0B11924g	XP_452060.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ppk19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100477	NM_001021195.2 Latest version!	Schizosaccharomyces pombe serine/threonine protein kinase Ppk19 (ppk19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppk19 ( NM_001021195.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595288.1 Schizosaccharomyces pombe serine/threonine protein kinase Ppk19 (ppk19), mRNA.

Price & Availability↑

CloneID	OSc100477
Clone ID Related Accession (Same CDS sequence)	NM_001021195.2
Accession Version	NM_001021195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	serine/threonine protein kinase Ppk19
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021195.1.

ATGGGGATTC AGCTTTCCAC TATCCAGACC CCCCAATTCC ATGAATTGTT CGAGGAAGAG 
CTTCCAGAAT ATCACAATGA AAGAAGTCTA GGGGATTCAC ATTTTTTGCG AACGTTTCGC 
ATGCAAGATC GCAAGGGATA TGATGTTTTA ATCAAAGTAT TTGTAAATAA ACTTCCGGAA 
ATTTCGTTGT CAAGCATTGT AAATTTGTTG AAGGAGGAGC AAGAGAATAT CTCATATAGA 
GTGCCAAATG CCGTACCCTA TATAAAAACG TTGGTTACGC TTCGCGCTGC CTACCTGGTG 
CGTCCATACG TGACTCACAA TTTGTATGAT AGAATTTCTA CTAGGCCCTT TCTTGAGTTG 
ACTGAAAAAA AGTGGATAAT GTTTCAATTA TTAAAGGGAA TTTCCGATTG TCATAGACTT 
GGTGTTTGTC ATGGTGATAT TAAATCGGAA AACATTTTAA TTACTTCTTG GAATTGGGCT 
TATCTATCGG ACTTTAGCTC TTTTAAACCT ACTTATTTGC CAGAGGATAA TCCTGCTGAC 
TACGGCTATT TTTTTGACAC TTCATCTCGA AGAGTCTGTA ATATCGCTCC CGAAAGATTT 
GTTCCTGCAT CGCAATTACA ACCTGCTCCA TTATCGCCTG CAATGGATAT TTTTAGTCTT 
GGATGTGTGT TTGCAGAACT GCTTTTGGAG GAGTCGCCTC TTTTTACGCT TTCTCAGCTT 
TTTAGCTACA AGGCCCATGG GTCTTACGAT TTACAGTCTG TTCTTGAGCA AATCGAGGAT 
AAGAGTACCC AAAACATGAT TCTAAGTATG CTAGACAGAG ATCCCAGTCA ACGATTGTCG 
GCTGATGCCT ATTTGCAGAA ATATCGAGGA ACCGTTTTTC CTGCATGCTT TTATGACACT 
TTGTATGATT ACTGTATTGG TTTAGTCGAT CCAAGCGGAA TTCTTTCTGC CAAGCAGCCT 
AATGACGCTT GCAGTTTGTA TGGTTCTCGA ATTGACGACA TTTACAGAGA TATTATGCCC 
CCAAATAATA ATGACATTTC AAACCTTATG CACACCCCTC ATGAATATAA TGGAATAGAT 
TCTTATTCTG TACCCCTTTT TACCACACTT GATGAATTTT ATAATAAAAA CCCTGCTGCC 
AAGAATTGGT ATCTAAGACT GTATAATACT ATGCAAGAAA AAAAAGGATT TGAGGATAAA 
GAGCAAGGTT CCACACCTGC ACCTTCTCCT TCAGTCATTG AGGATATTTC ATCTATTGAA 
ACGGATGAAA ATCATCTTTA TGGTGCCGCT GTTCTTCTTC CCATAGTTCT TTCCACTATT 
AGACACGTGA ATACAAGAGA ATCTAAGATA AACGCTTTAA GTTTGGTGCA AATTTTATCA 
AGGAACATCT GCGATGAGTC CAAACTTGAT ACAGTTTTAC CTTTTGTTAT GACTTTGCTT 
CGTGACCAAT ACGCCGATGT AAGGATTAGT GCGCTTATTA CGATTACTCG CCTAGTTAGT 
AATGTAACCT CTATTGCTCC GATTAATGCA TTTCTTTTTC AGGAGTATTT ATTTCCTGAC 
CTTCAGCATT TTCTTTTCGA TATGAATTCT CGTACTCGAG CAACATATGC ATCCTGCCTT 
CCAATTTTAG CCAAACAAGC TTCCAAATTT TTAAATTTGG CTCAATCATT AAGGAATGCT 
GGTATTTTAT CGTTTCCTGA ATCCGAATAT GAAAATATAA ATCATGGAAA GGCTGAATTG 
TTATTTGAAA CCGGTCGACA CGATTTAGTA GTTACTGTAG AAAGGCACGT TTCTACTCTT 
TTAGCGGACA GCTCTTCTAT TGTTCGTAGA TCTTTGCTGA ATGCTTTGGC TCCGCTTTGT 
GTTTTTTTCG GTAAAGCAAA GTCTAACGAT TTGATTTTGA GCCATCTGAT AACTTACTTG 
AATGACACGG ATTGGATGCT TCGTTGTGCA TTTTTTGAAA GTATTACCGG TCTATCCATT 
TTCATCGGTC CAAGGAGTGT AGATGAGTAT ATACTTCCCT TGATGCTGCA GGCTTTAGTA 
GATCCTGAGC CAGCTGTCCT TGAGAGTGTC TTGGGATCTT TTTCTGGACT GATCGAATTA 
CACTTATTTG AAAAGCTTGT TGTTGTTGAT ATCTTACAGC TTGTGCTTCC ACTTGTTGCT 
GTACCTAATG CTTATATTCG AAGAGCTGCC TTGTCTGTTA TTTACTCTGC ATATCAAAGT 
TTTGATGATA TTGATAGGGA CTGTATTGTA ACTCCTTTAT TAAGACCTTA CTTGATGTCC 
AATTTATGTG ATATAAACTC TTTAGAAAAG CTAGATCAAT TTATTTTGCC TATGGTATCT 
GATAGTGTTT GGTCTACTTT GACCCGCTGG TATGAAGAAT CCGAAAACTC AAGCTTTTGG 
AAGTGTGATA AAGATGCGTC TTACTCGTCT CTTGATGTAA GCAATGATTC GCTTTTAGGA 
AGAACCAAAA ATTTGCAAAA GAGAGAAATA ATGCACCACG CAGAAGTGTA TACTCACAAA 
AAAATCTCTC TAACTGGCCA TGTGATTATA ACTTCCACAG AAATGCTAGA GCTTAGTGAA 
GATGACCAGA AATGGGCTGA CATCATTAAA GAAATGGGAG TTGATGTAAA GCACTTATGG 
GTACTGGCTA ATTTGAGAGA TTATGTTAAG AAAACGAAAA TTAATTTAAA TGGAATCAAC 
TATAAACGAC AAAGTGGATT ACGGGAACTG AATACTTACC CTAATGCTCC CTTGCACATA 
TTACCAGAAA CCATTTTTTG GAACCCAGAA AGACCCCCTT CGTCTTCTAT CGCCAATGAA 
ACTTTCTTGG ACAGAAATTC TTATGCAGAA TCCATTCAAA CAAGCCGCTC CTATAACGAT 
GACTTAATTG CTCGAGATCC CATTGCATAT GTAGGGACGG ATATGACTAA TGCTTTTGGT 
ACGACCACAA AACCAAAAGA CGTTTCACAA TCAGATATCA AAGTTGATAT TAATAGGGAG 
TCTAATTCTG ATAATGGAGA GACCATTACT GGTACTCATG ATGTATACAG ACAAACGGAC 
AATCCTGAAA TTAAACTACC TTCAGACACT GCTAGCTCCA AGGTTGACAC ACACAATCCC 
ACAGTCACTC AACCAACGGA CGATACCGGT GGGTTGAATT CTTATAATAC TGAGAACCCC 
TTACTCACAA ATAATACTTT GGAGCCTTCC TCTGTAGAAG CAATAGTCTC TTCTAAAGAT 
TCTGACAAAC ATGCTAAAGA ATCGAAGGGG AAAAGCCTCG CACCGTTGAT CTCTTCTAGG 
GTTTCAACGA ATGATACAAC TAATGTTGCC GGTATTCGAA GCCAAAGATC ATTTACTACT 
CATATCGATT TGAAAAAGCT TACCACGAGG CAAAATGGCG CAAAAAAATC ATCGTATACT 
GGAACTAATC CATGCGTCTT AAATTATTTA AATAAAATTT ATGCAGAAGC AGCGGCTTCG 
ACACTTAACG TTGGTGCTGC TGTTTCACCA AGCTGGGCTT CTAATATACC TTTGCGTCGT 
AGAACAAAGG TGTCTGCGAA GGCTGCGAAT AAAATAGTTC AGACTCATGA ATGGCATCCA 
GAAGGATCAC GAGTTGCTCA GATATATTTA GGAAGCCTTC TTGATGGAGG TACGAAGAAA 
GTTTTGGTTA GTCCAGATTC ATCTTTTTTC GTTACCTTGG GATCTGACGG TGTTGTAAGA 
GCATGGCAAT TAGTTGAAAG TGTTCGTCAT ATTTCAACTA TGAGATGTGA ATGCCGATTA 
AGTTATGGGC ATACGCGACG TAATGGCGAG AGGAATAGAT TTTCTGTGGT CAATGGATGC 
TTTCTAGGTA ATACATACGC TTTTGCTTCT GTTACTCAAG ATGGCTCTGT GGAAGTTCAC 
AGACTTGACG TTAACAATCA GCGGCATACT CTAATTTCGG CTGGTCGTAT TCCCAATTTG 
GATTTCAGTG ATTCTGTAAC CTCCATGGAA GCTTCTACAT TTCACGACGG GAGTATCCGT 
CTAGTCGTTG TTACAAAATG GTCACGCATC GTATACTTAG ACGTGGGAAT GATGAGGGTT 
TTGTCATCAG ACCAATTACC ATTACAGTGT GGGTCAGCTA CTAGTGTAGT AGTTTCTGAG 
GGTTGTAATT GGGCTTTAAT TGGGACTACC AAAGGATGGC TCTTACTTTG GGACCTCCGC 
TTTGGGACAT TATCATGCTC TTGGCATATG CCAGCCAGGA TTGATCAGAT GCATCTTCTT 
TTAGATGTTA CCAAAAAACG TAGCAACGTT AATGAGTATA CTTCCGGAAA TAACAATAGT 
CCCGTTACCA AGGTTCCAGG TAGCTCAAGT ACAAGTTCAA GTAGTACTCA GCCAATTAAC 
TCTACTATTC CCATCAATCC TTTAGAAAAT CACGGAATGT TAAACTCGTT TGGAAGCACT 
ACAGTCAGTA TCAGTTTTAG CGTCCTTACC AACTTGGATA ATAAAGAAGT TAATCTTGAA 
GATGCAGTTC CAGCGAGTCA CAGATCCGCT TCAGGCATAG TAAATTTTGA CGTTGAAAAA 
GGGAAAACCG AAGAGGTTTT CTTAGAAAAC TGGAATTCTT CACTCACCAG TCCGATTCCG 
GTATCTGTTG GAATTGATGC TTTTAATGAA AAAAAAAAGT TTGATATAGA TTCTGGCAAT 
GACATGGGAT TAAGAGATTT GAATACCAAA TTCGACTCTC CTTGGCCTTG TATATCTAGT 
CCAATCTATA GGTACAGAGG TCCGTCTGCA GGTTCTGTCG AACGAGAGCC ACTTTTTCTC 
ATTGCCGCTT CTGGAAGCCC TCATGCGTTT ATTTGGAATC CCCACAATGT CTCTGCTAGT 
TCGTCGGTGA CCAACGACTC CGAGAGTTCA AAACTATCTT TAATACATAA CAAACCTCCT 
ATCTACCAAA AAGTCTCTGA ACAACAGAAT GTACGCCCAA AGTCTTCGGG AGTGTCAAGG 
CCTCTTCTAT TCCTTCAACA GCAAAAGAAT CTACCTAGCG AAAATCGTCT GCACCCCATC 
GTTGACATGG CCTTCTTGTA CCAACCGTAC GCTATAGTGC TTATTGTTGA CGCTTTTGGT 
AGTCTTGAAC TCTGGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595288.1
CDS	38..5158
Translation	MGIQLSTIQTPQFHELFEEELPEYHNERSLGDSHFLRTFRMQDRKGYDVLIKVFVNKLPEISLSSIVNLLKEEQENISYRVPNAVPYIKTLVTLRAAYLVRPYVTHNLYDRISTRPFLELTEKKWIMFQLLKGISDCHRLGVCHGDIKSENILITSWNWAYLSDFSSFKPTYLPEDNPADYGYFFDTSSRRVCNIAPERFVPASQLQPAPLSPAMDIFSLGCVFAELLLEESPLFTLSQLFSYKAHGSYDLQSVLEQIEDKSTQNMILSMLDRDPSQRLSADAYLQKYRGTVFPACFYDTLYDYCIGLVDPSGILSAKQPNDACSLYGSRIDDIYRDIMPPNNNDISNLMHTPHEYNGIDSYSVPLFTTLDEFYNKNPAAKNWYLRLYNTMQEKKGFEDKEQGSTPAPSPSVIEDISSIETDENHLYGAAVLLPIVLSTIRHVNTRESKINALSLVQILSRNICDESKLDTVLPFVMTLLRDQYADVRISALITITRLVSNVTSIAPINAFLFQEYLFPDLQHFLFDMNSRTRATYASCLPILAKQASKFLNLAQSLRNAGILSFPESEYENINHGKAELLFETGRHDLVVTVERHVSTLLADSSSIVRRSLLNALAPLCVFFGKAKSNDLILSHLITYLNDTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLGSFSGLIELHLFEKLVVVDILQLVLPLVAVPNAYIRRAALSVIYSAYQSFDDIDRDCIVTPLLRPYLMSNLCDINSLEKLDQFILPMVSDSVWSTLTRWYEESENSSFWKCDKDASYSSLDVSNDSLLGRTKNLQKREIMHHAEVYTHKKISLTGHVIITSTEMLELSEDDQKWADIIKEMGVDVKHLWVLANLRDYVKKTKINLNGINYKRQSGLRELNTYPNAPLHILPETIFWNPERPPSSSIANETFLDRNSYAESIQTSRSYNDDLIARDPIAYVGTDMTNAFGTTTKPKDVSQSDIKVDINRESNSDNGETITGTHDVYRQTDNPEIKLPSDTASSKVDTHNPTVTQPTDDTGGLNSYNTENPLLTNNTLEPSSVEAIVSSKDSDKHAKESKGKSLAPLISSRVSTNDTTNVAGIRSQRSFTTHIDLKKLTTRQNGAKKSSYTGTNPCVLNYLNKIYAEAAASTLNVGAAVSPSWASNIPLRRRTKVSAKAANKIVQTHEWHPEGSRVAQIYLGSLLDGGTKKVLVSPDSSFFVTLGSDGVVRAWQLVESVRHISTMRCECRLSYGHTRRNGERNRFSVVNGCFLGNTYAFASVTQDGSVEVHRLDVNNQRHTLISAGRIPNLDFSDSVTSMEASTFHDGSIRLVVVTKWSRIVYLDVGMMRVLSSDQLPLQCGSATSVVVSEGCNWALIGTTKGWLLLWDLRFGTLSCSWHMPARIDQMHLLLDVTKKRSNVNEYTSGNNNSPVTKVPGSSSTSSSSTQPINSTIPINPLENHGMLNSFGSTTVSISFSVLTNLDNKEVNLEDAVPASHRSASGIVNFDVEKGKTEEVFLENWNSSLTSPIPVSVGIDAFNEKKKFDIDSGNDMGLRDLNTKFDSPWPCISSPIYRYRGPSAGSVEREPLFLIAASGSPHAFIWNPHNVSASSSVTNDSESSKLSLIHNKPPIYQKVSEQQNVRPKSSGVSRPLLFLQQQKNLPSENRLHPIVDMAFLYQPYAIVLIVDAFGSLELWT

Target ORF information:

RefSeq Version	NM_001021195.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe serine/threonine protein kinase Ppk19 (ppk19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021195.2

ORF Insert Sequence:

ATGGGGATTC AGCTTTCCAC TATCCAGACC CCCCAATTCC ATGAATTGTT CGAGGAAGAG 
CTTCCAGAAT ATCACAATGA AAGAAGTCTA GGGGATTCAC ATTTTTTGCG AACGTTTCGC 
ATGCAAGATC GCAAGGGATA TGATGTTTTA ATCAAAGTAT TTGTAAATAA ACTTCCGGAA 
ATTTCGTTGT CAAGCATTGT AAATTTGTTG AAGGAGGAGC AAGAGAATAT CTCATATAGA 
GTGCCAAATG CCGTACCCTA TATAAAAACG TTGGTTACGC TTCGCGCTGC CTACCTGGTG 
CGTCCATACG TGACTCACAA TTTGTATGAT AGAATTTCTA CTAGGCCCTT TCTTGAGTTG 
ACTGAAAAAA AGTGGATAAT GTTTCAATTA TTAAAGGGAA TTTCCGATTG TCATAGACTT 
GGTGTTTGTC ATGGTGATAT TAAATCGGAA AACATTTTAA TTACTTCTTG GAATTGGGCT 
TATCTATCGG ACTTTAGCTC TTTTAAACCT ACTTATTTGC CAGAGGATAA TCCTGCTGAC 
TACGGCTATT TTTTTGACAC TTCATCTCGA AGAGTCTGTA ATATCGCTCC CGAAAGATTT 
GTTCCTGCAT CGCAATTACA ACCTGCTCCA TTATCGCCTG CAATGGATAT TTTTAGTCTT 
GGATGTGTGT TTGCAGAACT GCTTTTGGAG GAGTCGCCTC TTTTTACGCT TTCTCAGCTT 
TTTAGCTACA AGGCCCATGG GTCTTACGAT TTACAGTCTG TTCTTGAGCA AATCGAGGAT 
AAGAGTACCC AAAACATGAT TCTAAGTATG CTAGACAGAG ATCCCAGTCA ACGATTGTCG 
GCTGATGCCT ATTTGCAGAA ATATCGAGGA ACCGTTTTTC CTGCATGCTT TTATGACACT 
TTGTATGATT ACTGTATTGG TTTAGTCGAT CCAAGCGGAA TTCTTTCTGC CAAGCAGCCT 
AATGACGCTT GCAGTTTGTA TGGTTCTCGA ATTGACGACA TTTACAGAGA TATTATGCCC 
CCAAATAATA ATGACATTTC AAACCTTATG CACACCCCTC ATGAATATAA TGGAATAGAT 
TCTTATTCTG TACCCCTTTT TACCACACTT GATGAATTTT ATAATAAAAA CCCTGCTGCC 
AAGAATTGGT ATCTAAGACT GTATAATACT ATGCAAGAAA AAAAAGGATT TGAGGATAAA 
GAGCAAGGTT CCACACCTGC ACCTTCTCCT TCAGTCATTG AGGATATTTC ATCTATTGAA 
ACGGATGAAA ATCATCTTTA TGGTGCCGCT GTTCTTCTTC CCATAGTTCT TTCCACTATT 
AGACACGTGA ATACAAGAGA ATCTAAGATA AACGCTTTAA GTTTGGTGCA AATTTTATCA 
AGGAACATCT GCGATGAGTC CAAACTTGAT ACAGTTTTAC CTTTTGTTAT GACTTTGCTT 
CGTGACCAAT ACGCCGATGT AAGGATTAGT GCGCTTATTA CGATTACTCG CCTAGTTAGT 
AATGTAACCT CTATTGCTCC GATTAATGCA TTTCTTTTTC AGGAGTATTT ATTTCCTGAC 
CTTCAGCATT TTCTTTTCGA TATGAATTCT CGTACTCGAG CAACATATGC ATCCTGCCTT 
CCAATTTTAG CCAAACAAGC TTCCAAATTT TTAAATTTGG CTCAATCATT AAGGAATGCT 
GGTATTTTAT CGTTTCCTGA ATCCGAATAT GAAAATATAA ATCATGGAAA GGCTGAATTG 
TTATTTGAAA CCGGTCGACA CGATTTAGTA GTTACTGTAG AAAGGCACGT TTCTACTCTT 
TTAGCGGACA GCTCTTCTAT TGTTCGTAGA TCTTTGCTGA ATGCTTTGGC TCCGCTTTGT 
GTTTTTTTCG GTAAAGCAAA GTCTAACGAT TTGATTTTGA GCCATCTGAT AACTTACTTG 
AATGACACGG ATTGGATGCT TCGTTGTGCA TTTTTTGAAA GTATTACCGG TCTATCCATT 
TTCATCGGTC CAAGGAGTGT AGATGAGTAT ATACTTCCCT TGATGCTGCA GGCTTTAGTA 
GATCCTGAGC CAGCTGTCCT TGAGAGTGTC TTGGGATCTT TTTCTGGACT GATCGAATTA 
CACTTATTTG AAAAGCTTGT TGTTGTTGAT ATCTTACAGC TTGTGCTTCC ACTTGTTGCT 
GTACCTAATG CTTATATTCG AAGAGCTGCC TTGTCTGTTA TTTACTCTGC ATATCAAAGT 
TTTGATGATA TTGATAGGGA CTGTATTGTA ACTCCTTTAT TAAGACCTTA CTTGATGTCC 
AATTTATGTG ATATAAACTC TTTAGAAAAG CTAGATCAAT TTATTTTGCC TATGGTATCT 
GATAGTGTTT GGTCTACTTT GACCCGCTGG TATGAAGAAT CCGAAAACTC AAGCTTTTGG 
AAGTGTGATA AAGATGCGTC TTACTCGTCT CTTGATGTAA GCAATGATTC GCTTTTAGGA 
AGAACCAAAA ATTTGCAAAA GAGAGAAATA ATGCACCACG CAGAAGTGTA TACTCACAAA 
AAAATCTCTC TAACTGGCCA TGTGATTATA ACTTCCACAG AAATGCTAGA GCTTAGTGAA 
GATGACCAGA AATGGGCTGA CATCATTAAA GAAATGGGAG TTGATGTAAA GCACTTATGG 
GTACTGGCTA ATTTGAGAGA TTATGTTAAG AAAACGAAAA TTAATTTAAA TGGAATCAAC 
TATAAACGAC AAAGTGGATT ACGGGAACTG AATACTTACC CTAATGCTCC CTTGCACATA 
TTACCAGAAA CCATTTTTTG GAACCCAGAA AGACCCCCTT CGTCTTCTAT CGCCAATGAA 
ACTTTCTTGG ACAGAAATTC TTATGCAGAA TCCATTCAAA CAAGCCGCTC CTATAACGAT 
GACTTAATTG CTCGAGATCC CATTGCATAT GTAGGGACGG ATATGACTAA TGCTTTTGGT 
ACGACCACAA AACCAAAAGA CGTTTCACAA TCAGATATCA AAGTTGATAT TAATAGGGAG 
TCTAATTCTG ATAATGGAGA GACCATTACT GGTACTCATG ATGTATACAG ACAAACGGAC 
AATCCTGAAA TTAAACTACC TTCAGACACT GCTAGCTCCA AGGTTGACAC ACACAATCCC 
ACAGTCACTC AACCAACGGA CGATACCGGT GGGTTGAATT CTTATAATAC TGAGAACCCC 
TTACTCACAA ATAATACTTT GGAGCCTTCC TCTGTAGAAG CAATAGTCTC TTCTAAAGAT 
TCTGACAAAC ATGCTAAAGA ATCGAAGGGG AAAAGCCTCG CACCGTTGAT CTCTTCTAGG 
GTTTCAACGA ATGATACAAC TAATGTTGCC GGTATTCGAA GCCAAAGATC ATTTACTACT 
CATATCGATT TGAAAAAGCT TACCACGAGG CAAAATGGCG CAAAAAAATC ATCGTATACT 
GGAACTAATC CATGCGTCTT AAATTATTTA AATAAAATTT ATGCAGAAGC AGCGGCTTCG 
ACACTTAACG TTGGTGCTGC TGTTTCACCA AGCTGGGCTT CTAATATACC TTTGCGTCGT 
AGAACAAAGG TGTCTGCGAA GGCTGCGAAT AAAATAGTTC AGACTCATGA ATGGCATCCA 
GAAGGATCAC GAGTTGCTCA GATATATTTA GGAAGCCTTC TTGATGGAGG TACGAAGAAA 
GTTTTGGTTA GTCCAGATTC ATCTTTTTTC GTTACCTTGG GATCTGACGG TGTTGTAAGA 
GCATGGCAAT TAGTTGAAAG TGTTCGTCAT ATTTCAACTA TGAGATGTGA ATGCCGATTA 
AGTTATGGGC ATACGCGACG TAATGGCGAG AGGAATAGAT TTTCTGTGGT CAATGGATGC 
TTTCTAGGTA ATACATACGC TTTTGCTTCT GTTACTCAAG ATGGCTCTGT GGAAGTTCAC 
AGACTTGACG TTAACAATCA GCGGCATACT CTAATTTCGG CTGGTCGTAT TCCCAATTTG 
GATTTCAGTG ATTCTGTAAC CTCCATGGAA GCTTCTACAT TTCACGACGG GAGTATCCGT 
CTAGTCGTTG TTACAAAATG GTCACGCATC GTATACTTAG ACGTGGGAAT GATGAGGGTT 
TTGTCATCAG ACCAATTACC ATTACAGTGT GGGTCAGCTA CTAGTGTAGT AGTTTCTGAG 
GGTTGTAATT GGGCTTTAAT TGGGACTACC AAAGGATGGC TCTTACTTTG GGACCTCCGC 
TTTGGGACAT TATCATGCTC TTGGCATATG CCAGCCAGGA TTGATCAGAT GCATCTTCTT 
TTAGATGTTA CCAAAAAACG TAGCAACGTT AATGAGTATA CTTCCGGAAA TAACAATAGT 
CCCGTTACCA AGGTTCCAGG TAGCTCAAGT ACAAGTTCAA GTAGTACTCA GCCAATTAAC 
TCTACTATTC CCATCAATCC TTTAGAAAAT CACGGAATGT TAAACTCGTT TGGAAGCACT 
ACAGTCAGTA TCAGTTTTAG CGTCCTTACC AACTTGGATA ATAAAGAAGT TAATCTTGAA 
GATGCAGTTC CAGCGAGTCA CAGATCCGCT TCAGGCATAG TAAATTTTGA CGTTGAAAAA 
GGGAAAACCG AAGAGGTTTT CTTAGAAAAC TGGAATTCTT CACTCACCAG TCCGATTCCG 
GTATCTGTTG GAATTGATGC TTTTAATGAA AAAAAAAAGT TTGATATAGA TTCTGGCAAT 
GACATGGGAT TAAGAGATTT GAATACCAAA TTCGACTCTC CTTGGCCTTG TATATCTAGT 
CCAATCTATA GGTACAGAGG TCCGTCTGCA GGTTCTGTCG AACGAGAGCC ACTTTTTCTC 
ATTGCCGCTT CTGGAAGCCC TCATGCGTTT ATTTGGAATC CCCACAATGT CTCTGCTAGT 
TCGTCGGTGA CCAACGACTC CGAGAGTTCA AAACTATCTT TAATACATAA CAAACCTCCT 
ATCTACCAAA AAGTCTCTGA ACAACAGAAT GTACGCCCAA AGTCTTCGGG AGTGTCAAGG 
CCTCTTCTAT TCCTTCAACA GCAAAAGAAT CTACCTAGCG AAAATCGTCT GCACCCCATC 
GTTGACATGG CCTTCTTGTA CCAACCGTAC GCTATAGTGC TTATTGTTGA CGCTTTTGGT 
AGTCTTGAAC TCTGGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ppk19 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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