SPBC14F5.13c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPBC14F5.13c
Entrez Gene ID	2539926
Full Name	vacuolar membrane alkaline phosphatase (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022665.2	NP_596739.1	putative alkaline phosphatase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E12827g	XP_454528.1
Neurospora crassa	NCU08997	XP_959025.1
Eremothecium gossypii	AGOS_AGL260W	NP_986407.1
Schizosaccharomyces pombe (fission yeast)	SPBC14F5.13c	NP_596739.1
Saccharomyces cerevisiae (baker's yeast)	PHO8	NP_010769.3
Magnaporthe oryzae (rice blast fungus)	MGG_05299	XP_003712860.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Isp6p and Psp3p are functional in the vacuole and are involved in proteolytic processing of carboxypeptidase Y and alkaline phosphatase.
Title: Processing and maturation of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe.

The following SPBC14F5.13c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC14F5.13c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100670	NM_001022665.2 Latest version!	Schizosaccharomyces pombe putative alkaline phosphatase (SPBC14F5.13c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPBC14F5.13c ( NM_001022665.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596739.1 Schizosaccharomyces pombe putative alkaline phosphatase (SPBC14F5.13c), mRNA.

Price & Availability↑

CloneID	OSc100670
Clone ID Related Accession (Same CDS sequence)	NM_001022665.2
Accession Version	NM_001022665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative alkaline phosphatase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022665.1.

ATGGCATCTG AGCGCGATCC TCTTCTGCCA GTGCATGGCG AGGGTCCGGA GAGCCCTTCT 
CGTAGAAATT GGAAAACTTG GATAAAACAT GGAATTCTGT TAATCCTTGT TTTATCAACC 
GTTATATTTT TTTATTTTTT CTCGTCTCAT AAGTCCAAAG GGACCAATGA GAAACCAAAA 
TTTGTTATTA TGATGGTTTC TGACGGAATG GGACCTGGCT CTTTGTCTAT GACTAGATCG 
TTTGTAGAGA CTCTAAACGA TAAAGAGGGT TATAGGCTTC CCTTGGATGA GCATTTAATT 
GGCAGTTCAC GGACACGTTC CAGTTCGAGT TTGATTACGG ATAGTGCAGC CGGAGCAACC 
GCATTTTCTT GTGCTAATAA AACCTACAAC GGAGCTGTTG GTGTTTTGGA TAATGAAAAA 
CCTTGTGGAA CTATCTTAGA GGCTGCTAAA GAAGCCGGTT ACTTAACCGG AATTGTTGTC 
ACAAGCAGAG TTACCGACGC TACACCTGCC TCTTTCTCTG CTCATGCCGC GAATCGATTC 
ATGCAAGATT TAATTGCTGA GTACCAAGTC GGGATGGGAC CTTTGGGAAG GAGTGTTGAC 
TTATTGTTCG GTGGTGGACT TTGTTCATTC TTACCTAAAT CTACTTATCG ATCATGTAGA 
TCCGATAACT TGGATCTTTT GAAATATGCT AGGAAAAAAG AAGGTTTCCA AATTTTGCTT 
AATAGAACTG ACTTTGATGA GCTATCAAAC GCTCAATTGC CTTTGCTTGG TTTGTTTTCT 
GATTATCATC TCAGTTATGA TATTGATTAT CAGCCTGAAG TGCAACCTAA GCTTTCTGAA 
ATGGTAGAGA CGGCTTTGGA TGTTTTGCTC AACGCCACTA ATGAAGATAC ATCTAAAGGA 
TTTTTTTTGC TCATAGAAGG GAGTAGAATT GACATGGCCT CTCACAACAA TGACCCTATC 
GCTCATGTTT ACGAAGTTAT GGAATACAAC AGGGCCTTTG AAATAGCAAG TGCATTTGTA 
GAAAAGAATG GAGGATCTTT AATCTCTACA TCGGATCATG AAACTGGTGG TTTGACTGTT 
GGTCGCCAGG TCTCCAAAAA ATATCCAGAG TATTTATGGA AGCCGCAAGT CCTATCTCTT 
GCTCTCCATT CTATTGAATA TCTCGCTTCA GCTATCGTGA ACCATAACCA AAATACGTTG 
CTTCCTTATA TTGAACAGTT TGTTTTACCC GCAATTGGCA TTCCTGACCC TAATCCAAAG 
CAGATTCATG ACATATACGT GGCCCGGCAT AATATATTTA ATCTTATAAA CGTGCTTAGT 
GATATTGTTA GTGTAGAAGC ACAAATTGGC TGGACCACTC ACGGCCATAC TGCTGTCGAT 
GTAAACGTTT ATGGAGTAGG AGAGGTCACT GAACATCTTC GAGGAAATAT GGAGAACATT 
GAAATTGGAC AGTTTATGGA GATATACTTA AATGTTTCAC TAAGCGATGT AACTGAAAAG 
CTCAAAGATG CTCCCATACA TGGTGCCCCA GATCGACCTA GTTTAGTGGA AACTTCATTT 
TCCGATCGTC TTGTAGGTTT CGGTGCTGAT TTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596739.1
CDS	136..1734
Translation	MASERDPLLPVHGEGPESPSRRNWKTWIKHGILLILVLSTVIFFYFFSSHKSKGTNEKPKFVIMMVSDGMGPGSLSMTRSFVETLNDKEGYRLPLDEHLIGSSRTRSSSSLITDSAAGATAFSCANKTYNGAVGVLDNEKPCGTILEAAKEAGYLTGIVVTSRVTDATPASFSAHAANRFMQDLIAEYQVGMGPLGRSVDLLFGGGLCSFLPKSTYRSCRSDNLDLLKYARKKEGFQILLNRTDFDELSNAQLPLLGLFSDYHLSYDIDYQPEVQPKLSEMVETALDVLLNATNEDTSKGFFLLIEGSRIDMASHNNDPIAHVYEVMEYNRAFEIASAFVEKNGGSLISTSDHETGGLTVGRQVSKKYPEYLWKPQVLSLALHSIEYLASAIVNHNQNTLLPYIEQFVLPAIGIPDPNPKQIHDIYVARHNIFNLINVLSDIVSVEAQIGWTTHGHTAVDVNVYGVGEVTEHLRGNMENIEIGQFMEIYLNVSLSDVTEKLKDAPIHGAPDRPSLVETSFSDRLVGFGADLF

Target ORF information:

RefSeq Version	NM_001022665.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative alkaline phosphatase (SPBC14F5.13c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022665.2

ORF Insert Sequence:

ATGGCATCTG AGCGCGATCC TCTTCTGCCA GTGCATGGCG AGGGTCCGGA GAGCCCTTCT 
CGTAGAAATT GGAAAACTTG GATAAAACAT GGAATTCTGT TAATCCTTGT TTTATCAACC 
GTTATATTTT TTTATTTTTT CTCGTCTCAT AAGTCCAAAG GGACCAATGA GAAACCAAAA 
TTTGTTATTA TGATGGTTTC TGACGGAATG GGACCTGGCT CTTTGTCTAT GACTAGATCG 
TTTGTAGAGA CTCTAAACGA TAAAGAGGGT TATAGGCTTC CCTTGGATGA GCATTTAATT 
GGCAGTTCAC GGACACGTTC CAGTTCGAGT TTGATTACGG ATAGTGCAGC CGGAGCAACC 
GCATTTTCTT GTGCTAATAA AACCTACAAC GGAGCTGTTG GTGTTTTGGA TAATGAAAAA 
CCTTGTGGAA CTATCTTAGA GGCTGCTAAA GAAGCCGGTT ACTTAACCGG AATTGTTGTC 
ACAAGCAGAG TTACCGACGC TACACCTGCC TCTTTCTCTG CTCATGCCGC GAATCGATTC 
ATGCAAGATT TAATTGCTGA GTACCAAGTC GGGATGGGAC CTTTGGGAAG GAGTGTTGAC 
TTATTGTTCG GTGGTGGACT TTGTTCATTC TTACCTAAAT CTACTTATCG ATCATGTAGA 
TCCGATAACT TGGATCTTTT GAAATATGCT AGGAAAAAAG AAGGTTTCCA AATTTTGCTT 
AATAGAACTG ACTTTGATGA GCTATCAAAC GCTCAATTGC CTTTGCTTGG TTTGTTTTCT 
GATTATCATC TCAGTTATGA TATTGATTAT CAGCCTGAAG TGCAACCTAA GCTTTCTGAA 
ATGGTAGAGA CGGCTTTGGA TGTTTTGCTC AACGCCACTA ATGAAGATAC ATCTAAAGGA 
TTTTTTTTGC TCATAGAAGG GAGTAGAATT GACATGGCCT CTCACAACAA TGACCCTATC 
GCTCATGTTT ACGAAGTTAT GGAATACAAC AGGGCCTTTG AAATAGCAAG TGCATTTGTA 
GAAAAGAATG GAGGATCTTT AATCTCTACA TCGGATCATG AAACTGGTGG TTTGACTGTT 
GGTCGCCAGG TCTCCAAAAA ATATCCAGAG TATTTATGGA AGCCGCAAGT CCTATCTCTT 
GCTCTCCATT CTATTGAATA TCTCGCTTCA GCTATCGTGA ACCATAACCA AAATACGTTG 
CTTCCTTATA TTGAACAGTT TGTTTTACCC GCAATTGGCA TTCCTGACCC TAATCCAAAG 
CAGATTCATG ACATATACGT GGCCCGGCAT AATATATTTA ATCTTATAAA CGTGCTTAGT 
GATATTGTTA GTGTAGAAGC ACAAATTGGC TGGACCACTC ACGGCCATAC TGCTGTCGAT 
GTAAACGTTT ATGGAGTAGG AGAGGTCACT GAACATCTTC GAGGAAATAT GGAGAACATT 
GAAATTGGAC AGTTTATGGA GATATACTTA AATGTTTCAC TAAGCGATGT AACTGAAAAG 
CTCAAAGATG CTCCCATACA TGGTGCCCCA GATCGACCTA GTTTAGTGGA AACTTCATTT 
TCCGATCGTC TTGTAGGTTT CGGTGCTGAT TTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPBC14F5.13c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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