top3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following top3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100170 NM_001023781.2
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Schizosaccharomyces pombe DNA topoisomerase III (top3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100170
    Clone ID Related Accession (Same CDS sequence) NM_001023781.2
    Accession Version NM_001023781.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product DNA topoisomerase III
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001023781.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGCGTCC TATGTGTTGC TGAAAAAAAC TCGATTGCCA AATCGGTAGC AAGCATTCTA 
    GGGGGTGGAC ACGTGAGAAG GAGAGATACA CGAAGTAAGT ATGTAAAAAA TTACGACTTC
    TCATTTAATT TTGGAGGCAA TGTTGGATCT TCTGATGTTA CAATGACTTC TGTATCCGGT
    CACTTAACAG AAGCTTCTTT TCCTTCTGAA TATTCTAGTT GGAGCTCTGT CCCTCAAGAC
    GTTTTATTCG ATGCTCAAAT CATCACCTCA GTATCAAAGA ATGCAGAAGT ACTAGCAGAC
    AATATTAAAA AAGAAGCTAG AAATGCCCAG TACCTTTATA TTTGGACCGA TTGTGATCGT
    GAGGGTGAAC ACATTGGGGT GGAAATCTCC AATGTAGCTA GAGCCAGCAA TCCATCTATT
    CAGGTTATCC GAGCAGACTT CAATAATTTG GAACGGTCAC ATATTATCAG TGCCGCGAAA
    CGTCCAAGAG ATGTTTCAAA AAATGCTGCA GATGCTGTGG ATGCTAGAAT TGAACTTGAT
    TTTCGTTTAG GTGCAATATT CACTCGGCTA CAAACTATTC AATTACAAAA ATCATTCGAT
    ATTCTTCAGA ATAAAATAAT AAGTTATGGT CCCTGCCAAT TCCCTACATT AGGCTTTGTA
    GTTGATCGGT GGCAGCGAGT AGAAGACTTT GTGCCTGAAA CCTACTGGCA CCTTCGATTT
    GTTGATAAAA GACAGGGTAA GACTATTCAG TTCAATTGGG AAAGGGCCAA AGTTTTTGAC
    AGATTAACTA CAATGATCAT CTTGGAAAAC TGTTTAGAAT GCAAAACTGC TAAGGTCGTT
    AACATAACTC AAAAACCAAA AACCAAGTAT AAACCTCTGC CATTATCAAC TGTAGAACTT
    ACAAAACTGG GGCCAAAACA TCTAAGAATT TCTGCTAAGA AAACTTTAGA GCTCGCCGAA
    AACTTATATA CTAATGGGTT TGTTTCTTAC CCACGTACTG AAACCGACCA ATTTGACTCT
    TCAATGAATT TGCATGCGAT TATCCAAAAA CTAACAGGTG CACAAGAATG GGACTCTTAT
    GCAGAAGGGC TTTTGGCTGG AGATTATCGA CCGCCCCGAA AAGGTAAACA CAATGATCGC
    GCTCATCCAC CTATTCATCC CGTTCAAATG GTTCACCGGT CAGCGCTTCC TTCCCAAGAT
    CATTGGAAAG TATATGAGCT AATTACAAGA AGATTTTTAG CTTGTTGTTC TGACAATGCC
    AAAGGAGCCG AAACGTTGGT GCAGGTTAAA ATGGAAGAAG AGCTTTTTTC TAAAAAGGGA
    TTGCTAGTGA CAGAGAAAAA TTATTTAGAG GTTTATCCAT ATGAGAAATG GGAAAGTTCG
    GATCAATTAC CAGAGTATAG ACTTCACGAG GAATTCCAAC CCCACATTCT CGACATGATG
    GACAGTTCAA CGTCCTCCCC TTCCTATATC ACTGAACCGG AGTTAATTGC CTTGATGGAT
    GCCAATGGAA TCGGGACAGA TGCTACTATG GCAGAGCACA TCGAAAAAGT TCAGGAGAGA
    GAATATGTCA TAAAACGAAA AAAAAGGGGC CAAGGTGTAA CTGAATTTGT ACCAAGCTCT
    TTAGGAGTTG CTTTGGCAAA AGGCTATGAT GAAATTGGTT TAGAGTGGAG TTTGACCAAG
    CCTTTCTTGC GAAAAGAAAT GGAAGTTCAA CTTAAAAACA TAGAAAATGG CCAACTTAAT
    CGGAATGTTC TAGTTCATAT GATCCTTACC CAATTCCGTG ATGTTTTTCA TCTCACAAAG
    CAAAGATTCG ATTGTTTAAA AAATAGTTGC AGAGTCTACC TTATGAGTCA TAATGAACCG
    CAAACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596761.1
    CDS296..2164
    Translation

    Target ORF information:

    RefSeq Version NM_001023781.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe DNA topoisomerase III (top3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGCGTCC TATGTGTTGC TGAAAAAAAC TCGATTGCCA AATCGGTAGC AAGCATTCTA 
    GGGGGTGGAC ACGTGAGAAG GAGAGATACA CGAAGTAAGT ATGTAAAAAA TTACGACTTC
    TCATTTAATT TTGGAGGCAA TGTTGGATCT TCTGATGTTA CAATGACTTC TGTATCCGGT
    CACTTAACAG AAGCTTCTTT TCCTTCTGAA TATTCTAGTT GGAGCTCTGT CCCTCAAGAC
    GTTTTATTCG ATGCTCAAAT CATCACCTCA GTATCAAAGA ATGCAGAAGT ACTAGCAGAC
    AATATTAAAA AAGAAGCTAG AAATGCCCAG TACCTTTATA TTTGGACCGA TTGTGATCGT
    GAGGGTGAAC ACATTGGGGT GGAAATCTCC AATGTAGCTA GAGCCAGCAA TCCATCTATT
    CAGGTTATCC GAGCAGACTT CAATAATTTG GAACGGTCAC ATATTATCAG TGCCGCGAAA
    CGTCCAAGAG ATGTTTCAAA AAATGCTGCA GATGCTGTGG ATGCTAGAAT TGAACTTGAT
    TTTCGTTTAG GTGCAATATT CACTCGGCTA CAAACTATTC AATTACAAAA ATCATTCGAT
    ATTCTTCAGA ATAAAATAAT AAGTTATGGT CCCTGCCAAT TCCCTACATT AGGCTTTGTA
    GTTGATCGGT GGCAGCGAGT AGAAGACTTT GTGCCTGAAA CCTACTGGCA CCTTCGATTT
    GTTGATAAAA GACAGGGTAA GACTATTCAG TTCAATTGGG AAAGGGCCAA AGTTTTTGAC
    AGATTAACTA CAATGATCAT CTTGGAAAAC TGTTTAGAAT GCAAAACTGC TAAGGTCGTT
    AACATAACTC AAAAACCAAA AACCAAGTAT AAACCTCTGC CATTATCAAC TGTAGAACTT
    ACAAAACTGG GGCCAAAACA TCTAAGAATT TCTGCTAAGA AAACTTTAGA GCTCGCCGAA
    AACTTATATA CTAATGGGTT TGTTTCTTAC CCACGTACTG AAACCGACCA ATTTGACTCT
    TCAATGAATT TGCATGCGAT TATCCAAAAA CTAACAGGTG CACAAGAATG GGACTCTTAT
    GCAGAAGGGC TTTTGGCTGG AGATTATCGA CCGCCCCGAA AAGGTAAACA CAATGATCGC
    GCTCATCCAC CTATTCATCC CGTTCAAATG GTTCACCGGT CAGCGCTTCC TTCCCAAGAT
    CATTGGAAAG TATATGAGCT AATTACAAGA AGATTTTTAG CTTGTTGTTC TGACAATGCC
    AAAGGAGCCG AAACGTTGGT GCAGGTTAAA ATGGAAGAAG AGCTTTTTTC TAAAAAGGGA
    TTGCTAGTGA CAGAGAAAAA TTATTTAGAG GTTTATCCAT ATGAGAAATG GGAAAGTTCG
    GATCAATTAC CAGAGTATAG ACTTCACGAG GAATTCCAAC CCCACATTCT CGACATGATG
    GACAGTTCAA CGTCCTCCCC TTCCTATATC ACTGAACCGG AGTTAATTGC CTTGATGGAT
    GCCAATGGAA TCGGGACAGA TGCTACTATG GCAGAGCACA TCGAAAAAGT TCAGGAGAGA
    GAATATGTCA TAAAACGAAA AAAAAGGGGC CAAGGTGTAA CTGAATTTGT ACCAAGCTCT
    TTAGGAGTTG CTTTGGCAAA AGGCTATGAT GAAATTGGTT TAGAGTGGAG TTTGACCAAG
    CCTTTCTTGC GAAAAGAAAT GGAAGTTCAA CTTAAAAACA TAGAAAATGG CCAACTTAAT
    CGGAATGTTC TAGTTCATAT GATCCTTACC CAATTCCGTG ATGTTTTTCA TCTCACAAAG
    CAAAGATTCG ATTGTTTAAA AAATAGTTGC AGAGTCTACC TTATGAGTCA TAATGAACCG
    CAAACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L