ste20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ste20
Entrez Gene ID	2539845
Full Name	target of rapamycin complex 2 subunit ste20
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021929.2	NP_596021.1	target of rapamycin complex 2 subunit ste20

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E20131g	XP_454862.1
Eremothecium gossypii	AGOS_ABR214C	NP_983163.2
Magnaporthe oryzae (rice blast fungus)	MGG_05284	XP_003712844.1
Saccharomyces cerevisiae (baker's yeast)	TSC11	NP_011018.1
Schizosaccharomyces pombe (fission yeast)	ste20	NP_596021.1
Neurospora crassa	NCU07854	XP_963115.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ste20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ste20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101674	NM_001021929.2 Latest version!	Schizosaccharomyces pombe target of rapamycin complex 2 subunit ste20 (ste20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ste20 ( NM_001021929.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596021.1 Schizosaccharomyces pombe target of rapamycin complex 2 subunit ste20 (ste20), mRNA.

Price & Availability↑

CloneID	OSc101674
Clone ID Related Accession (Same CDS sequence)	NM_001021929.2
Accession Version	NM_001021929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	target of rapamycin complex 2 subunit ste20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021929.1.

ATGAAACCTG TGCGAAGGGG ACAAACCGAT ACTGCTTTGG ATATAAGTTC CCATGCCAAA 
ACTAATGGTG ACTTTATCAA AAAAATGAAT ACCACTGATT CAAAAAGGCT CAAGTTACTT 
GAGGATTTGA AGGGAAAGCT CGAAGTTGAG TGCAAAATAC GGGATGGCGC TGAAACGTTG 
CTGCAAGTCT TCGACACCAA TTTTAAAAAA GAAACAAAAG AACGTAAAGA AATGTTAAAA 
AAGAAATGTA CCGATGAGCT CGAATCATCT AAAAAAAAGA TAGAGGAATT AGTTTCTAGC 
ATTGAATCTT TCCAAGGAGA AAATGGTGAA GCAAAAACTG GCTCAACCAG TCTCACGAGG 
TCTGCTTCTG CGACTGTCAG TCGAAAGAGC TCATTGCAGG AAAAGTATAG TACGAGATTT 
TCTTACAAAG CAGGATGTAG CGATAGTTGC AGTGTTACAG TCAGTGGGAC TGGTGAATTA 
ATAGGTCCTA CCAGAAATGC GCATAGCAAT TTAACTCCTA CCGTCATTCA AAGAATTGAC 
TTTGAAAATG TCAACGAAAA AAATAATTCT TCTTCTGAAG ATACTCAACC TAACGGAAAA 
CGTCCTTCTA GTTTGCAATC GAATTTCTCA CAATTTCCAT TAAATCCTTG GCTTGATAAT 
ATATATAAGG CTTGTCTTGA AGGCAGTATG AAGGATGTAA TCGATTCCTC AAATAATTTA 
TGTGAATACT TACATGAACA TTCTGATCCC GCTTATGCAA AAAACTTCTC CTTGATTACT 
CCGACCATAC TTTCAATGCT CGAGTTGAAT GTCTCTGAAG TTACGGCTTC TGTTTATAGA 
CTACTAAGGC ATTTGTTTCT TGACGCAACA GCATTCTCTT GTTGTCAAAT GTTGAATTTA 
CCTTGGATCT TGTCAAAGTC TCTACTTTCT GGTACCGATG CATATCAAAT TGAACGAGAG 
CAAGCATTCC GACTAATCCG AACTCTTTAT TTTCTTTCAT CAACTGAAGG ACATGAGGAT 
TATCTTTCAG GTATTACGAG GACTATCATT TCAATTTGTG AGCATGTATC AGATGTTAGT 
CGTGGAATTG CTGTTGAAAC TCTTATCGAG TTAATGATTA TTCGACCAAA AATTTTATTT 
AAAGCTAATG GTTTAAGGGT TTTAATGATC TCCCTAATCG ATGGAAGTAT TTCTGAGAAT 
TTAGCAGCTA GTGCTGCACT TGCATTAGTA TATCTTTTGG ATGATCCTGA AAGCGCTTGT 
TACGTAAATT TACCCTACGA TATTGGCATT TTACTTTCAC CTTTTACTAG TTCTTCTTCG 
CGAGATACAT TTAATTCCTC TGAGGAACAG TCTGAACAAG CTGCAAAAGC CATGAAATCT 
TCCGCCAAAG TTGCCTCTGT TTTGCTTAAT TCATGGTCTG GATTATTGGC TTTGTCTACC 
AATGATTTTC AGGCTCTTCG GTCGATTGTT GATACACTTC GGGTTCCATC CTTTGCTCCA 
CGAAGCGATG TAATTGATCT TTTCTTTTTA ATTTTCCAAG TTGAGTATTC AAGTTGGTCA 
GAGTCTTTTT TGGCCGGAAA ACGATTAACA GTCGTTAAAA ATCAGGCTGT ATCTAATGAT 
GATAACATAA ACATGGTTAA TATACCGGAT GGTTCCAACA AAAAGTACAT GTCGCTACGC 
CAACATTTTA CAGCCGTCTT ATTATTTATA TTTTTGGAGC TCGGTTTGGT TGAATCTATT 
GTATGTATGA TTAGGGCCAG CGATGACCCA TCAGCCTCAC GTAAAGCTAC ATATCTTCTA 
GGAGAGGTAT TGAGGCTGTC TGATGAGCTA CTGCCGATTC ATTTAGGAGC CAAAATACAA 
TCCTTGCCAT CTTTATTTAA TATGGCTTCT CAATTTACTG CCGAAGACAG GTTTGTTGCC 
ACTTCTGTTC TTCAATCCAT TGAAAGTTTG AATAGAGTTA AATTCCATTC TGCAACACAA 
CCTTTCTCGC AGACAACCTC TCTTCTTTTT AAGGAGCAAA AAACTGATGG ATCTTTTCGG 
GGGCAAAGAC AGGTTGAGCA TGTTAAATTG AAAATGGGCA TGCAGATTGA TGATTCACAT 
TTTCGTAGTA TGCTAGCTGA AACCAACGTG TTAGCTACAA AAAACTATCA AAAATGGCGA 
TGGGATACAC TTGTCCAAAT TATGGAGGGT CCATTGCTAA GTCCGAAACG TATTGATGAA 
ACTTTGCGAA CCACAAAATT TATGAGACGG CTTTTAGCAT TTTATAAACC CTTTTCCAAC 
AGATTTTCTT CCATACAAAA CACTAAACCA AATCAAAAAT TTATAAAGGT TGGTTGTCTT 
GTTTTCAGGA CTTTACTAGC CAATCCGGAA GGCGTTAAAT ATCTTTCTGA AAGCAAAGTG 
ATTAAACAAA TAGCAGAATC TCTTAGCCAG ATAGACGGAT ATTCAGAACA GGTTTCTGAG 
CCGATTTTCT CGAACAGTAG GCTTCAGAAA ACCCTCACCC ACGGTTACTT TCCTATGTTA 
AAAGTATTGT CAAGCCAAAA GGAAGGTCAT GCAATTATGG AACGATGGAG GATTTTTACT 
ACACTTTACC ATTTGACAGA ATTGAGGAAC AGAGACGATC TCATAATTAT TTTTCTGACT 
AATCTTGATT ATCGGCTAGA GGGGCACACA CGAATTATAT TTTCTAAGGC GCTTAATACC 
GGGCAACAGG CTGTACGCTT AACTGCAACC AAGCATCTTG CGGCTTTAAT CAATTCTGAG 
TCTGCCAATG ACAACTTAAA TCACTGGGCA ATTTCTCTAT TGATATTTCA ACTGTACGAT 
CCTTGCTTGG AAGTATGTAA AACAGCAGTG AAGGTGTTGA ATGAAGTTTG TGCTCGAAAT 
GAAAACTTGC TGGCTCAAGT TGTTCAGCTT CAACCTTCAT TGGCCCATTT GGGTGAAATT 
GGCTCACCTT TACTTTTACG TTTTCTTGCT ACAACTGTCG GATTTCATTA CCTTTCTGAA 
ATTAATTTTA TTGAACATGA ACTCGATAAT TGGTACCATC ATCGAAATAT AGATTACGTG 
GATTTATTGG AGCAAAATTT TTTCCTTTCT TTTGTTTCAA ATCTCAAAAT TATTGACAAG 
AAAAACAATG AACCAGATGA AAATATACTT CCCCTACACT TTTATGGCGA ATTAGTCAAA 
TCTCCACAAG GTTGTGAGGT TCTCGAGTCT TCTGGGCATT TTGAATCTTT TATGGGTACC 
TTGGTGGAAT TTTACGATAA ACCGTTAGGA AATGAAGCCA TCAGGCAGTT AAAGAGTGCT 
CTTTGGGCTA TAGGAAACAT TGGAAAAACC GATCAGGGTA TTACGTTTTT AATTAACCAT 
GATACGATAC CGTTGATTGT CAAATATGCC GAAAATTCAT TGATTCCTAC TGTTCGAGGA 
ACTGCTTATT TTGTATTAGG CTTGATTTCC AGGACTTCTA AAGGCGTTGA GATACTCGAA 
TCACTGCATT GGTATAGCCT TATGTCTTTA ATGGGCACTT CACAAGGAAT TTGCATTCCG 
AGACATGCTG GACAAGTATT ATCAACTCCA AGGAGAAATG TTGAGTTTGT AAATGAGCGC 
GTGCCAACTC CTGAGTTTTC TTCATTACTT AGCTCTTTAA CTAACTCAGA GAGAGAGGTA 
ATTAGGCTCG TTTCCAATTT GTCAAACCAT GTTTTAACAA ACGAGTCAGC ACGGCAACTA 
ACAAAGATTC GTTCGAAAAA TGCTAAAGTT TTCTCCTCTA AACGTTTGGT GAAAGCTTGT 
ATGACCATTC TGGGAAAATT CCATTACCGT GTTCAGATTC AACAATTTGT ATTTGAATTA 
TTTCCTTACT CGGTTTTATT GTCTTCCTCA ACCTCACAGG ACTTAAATGA ATCCCCGTCC 
AGGCCGAATA ATTTGTCAAT TTCCGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596021.1
CDS	270..4199
Translation	MKPVRRGQTDTALDISSHAKTNGDFIKKMNTTDSKRLKLLEDLKGKLEVECKIRDGAETLLQVFDTNFKKETKERKEMLKKKCTDELESSKKKIEELVSSIESFQGENGEAKTGSTSLTRSASATVSRKSSLQEKYSTRFSYKAGCSDSCSVTVSGTGELIGPTRNAHSNLTPTVIQRIDFENVNEKNNSSSEDTQPNGKRPSSLQSNFSQFPLNPWLDNIYKACLEGSMKDVIDSSNNLCEYLHEHSDPAYAKNFSLITPTILSMLELNVSEVTASVYRLLRHLFLDATAFSCCQMLNLPWILSKSLLSGTDAYQIEREQAFRLIRTLYFLSSTEGHEDYLSGITRTIISICEHVSDVSRGIAVETLIELMIIRPKILFKANGLRVLMISLIDGSISENLAASAALALVYLLDDPESACYVNLPYDIGILLSPFTSSSSRDTFNSSEEQSEQAAKAMKSSAKVASVLLNSWSGLLALSTNDFQALRSIVDTLRVPSFAPRSDVIDLFFLIFQVEYSSWSESFLAGKRLTVVKNQAVSNDDNINMVNIPDGSNKKYMSLRQHFTAVLLFIFLELGLVESIVCMIRASDDPSASRKATYLLGEVLRLSDELLPIHLGAKIQSLPSLFNMASQFTAEDRFVATSVLQSIESLNRVKFHSATQPFSQTTSLLFKEQKTDGSFRGQRQVEHVKLKMGMQIDDSHFRSMLAETNVLATKNYQKWRWDTLVQIMEGPLLSPKRIDETLRTTKFMRRLLAFYKPFSNRFSSIQNTKPNQKFIKVGCLVFRTLLANPEGVKYLSESKVIKQIAESLSQIDGYSEQVSEPIFSNSRLQKTLTHGYFPMLKVLSSQKEGHAIMERWRIFTTLYHLTELRNRDDLIIIFLTNLDYRLEGHTRIIFSKALNTGQQAVRLTATKHLAALINSESANDNLNHWAISLLIFQLYDPCLEVCKTAVKVLNEVCARNENLLAQVVQLQPSLAHLGEIGSPLLLRFLATTVGFHYLSEINFIEHELDNWYHHRNIDYVDLLEQNFFLSFVSNLKIIDKKNNEPDENILPLHFYGELVKSPQGCEVLESSGHFESFMGTLVEFYDKPLGNEAIRQLKSALWAIGNIGKTDQGITFLINHDTIPLIVKYAENSLIPTVRGTAYFVLGLISRTSKGVEILESLHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNVEFVNERVPTPEFSSLLSSLTNSEREVIRLVSNLSNHVLTNESARQLTKIRSKNAKVFSSKRLVKACMTILGKFHYRVQIQQFVFELFPYSVLLSSSTSQDLNESPSRPNNLSISA

Target ORF information:

RefSeq Version	NM_001021929.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe target of rapamycin complex 2 subunit ste20 (ste20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021929.2

ORF Insert Sequence:

ATGAAACCTG TGCGAAGGGG ACAAACCGAT ACTGCTTTGG ATATAAGTTC CCATGCCAAA 
ACTAATGGTG ACTTTATCAA AAAAATGAAT ACCACTGATT CAAAAAGGCT CAAGTTACTT 
GAGGATTTGA AGGGAAAGCT CGAAGTTGAG TGCAAAATAC GGGATGGCGC TGAAACGTTG 
CTGCAAGTCT TCGACACCAA TTTTAAAAAA GAAACAAAAG AACGTAAAGA AATGTTAAAA 
AAGAAATGTA CCGATGAGCT CGAATCATCT AAAAAAAAGA TAGAGGAATT AGTTTCTAGC 
ATTGAATCTT TCCAAGGAGA AAATGGTGAA GCAAAAACTG GCTCAACCAG TCTCACGAGG 
TCTGCTTCTG CGACTGTCAG TCGAAAGAGC TCATTGCAGG AAAAGTATAG TACGAGATTT 
TCTTACAAAG CAGGATGTAG CGATAGTTGC AGTGTTACAG TCAGTGGGAC TGGTGAATTA 
ATAGGTCCTA CCAGAAATGC GCATAGCAAT TTAACTCCTA CCGTCATTCA AAGAATTGAC 
TTTGAAAATG TCAACGAAAA AAATAATTCT TCTTCTGAAG ATACTCAACC TAACGGAAAA 
CGTCCTTCTA GTTTGCAATC GAATTTCTCA CAATTTCCAT TAAATCCTTG GCTTGATAAT 
ATATATAAGG CTTGTCTTGA AGGCAGTATG AAGGATGTAA TCGATTCCTC AAATAATTTA 
TGTGAATACT TACATGAACA TTCTGATCCC GCTTATGCAA AAAACTTCTC CTTGATTACT 
CCGACCATAC TTTCAATGCT CGAGTTGAAT GTCTCTGAAG TTACGGCTTC TGTTTATAGA 
CTACTAAGGC ATTTGTTTCT TGACGCAACA GCATTCTCTT GTTGTCAAAT GTTGAATTTA 
CCTTGGATCT TGTCAAAGTC TCTACTTTCT GGTACCGATG CATATCAAAT TGAACGAGAG 
CAAGCATTCC GACTAATCCG AACTCTTTAT TTTCTTTCAT CAACTGAAGG ACATGAGGAT 
TATCTTTCAG GTATTACGAG GACTATCATT TCAATTTGTG AGCATGTATC AGATGTTAGT 
CGTGGAATTG CTGTTGAAAC TCTTATCGAG TTAATGATTA TTCGACCAAA AATTTTATTT 
AAAGCTAATG GTTTAAGGGT TTTAATGATC TCCCTAATCG ATGGAAGTAT TTCTGAGAAT 
TTAGCAGCTA GTGCTGCACT TGCATTAGTA TATCTTTTGG ATGATCCTGA AAGCGCTTGT 
TACGTAAATT TACCCTACGA TATTGGCATT TTACTTTCAC CTTTTACTAG TTCTTCTTCG 
CGAGATACAT TTAATTCCTC TGAGGAACAG TCTGAACAAG CTGCAAAAGC CATGAAATCT 
TCCGCCAAAG TTGCCTCTGT TTTGCTTAAT TCATGGTCTG GATTATTGGC TTTGTCTACC 
AATGATTTTC AGGCTCTTCG GTCGATTGTT GATACACTTC GGGTTCCATC CTTTGCTCCA 
CGAAGCGATG TAATTGATCT TTTCTTTTTA ATTTTCCAAG TTGAGTATTC AAGTTGGTCA 
GAGTCTTTTT TGGCCGGAAA ACGATTAACA GTCGTTAAAA ATCAGGCTGT ATCTAATGAT 
GATAACATAA ACATGGTTAA TATACCGGAT GGTTCCAACA AAAAGTACAT GTCGCTACGC 
CAACATTTTA CAGCCGTCTT ATTATTTATA TTTTTGGAGC TCGGTTTGGT TGAATCTATT 
GTATGTATGA TTAGGGCCAG CGATGACCCA TCAGCCTCAC GTAAAGCTAC ATATCTTCTA 
GGAGAGGTAT TGAGGCTGTC TGATGAGCTA CTGCCGATTC ATTTAGGAGC CAAAATACAA 
TCCTTGCCAT CTTTATTTAA TATGGCTTCT CAATTTACTG CCGAAGACAG GTTTGTTGCC 
ACTTCTGTTC TTCAATCCAT TGAAAGTTTG AATAGAGTTA AATTCCATTC TGCAACACAA 
CCTTTCTCGC AGACAACCTC TCTTCTTTTT AAGGAGCAAA AAACTGATGG ATCTTTTCGG 
GGGCAAAGAC AGGTTGAGCA TGTTAAATTG AAAATGGGCA TGCAGATTGA TGATTCACAT 
TTTCGTAGTA TGCTAGCTGA AACCAACGTG TTAGCTACAA AAAACTATCA AAAATGGCGA 
TGGGATACAC TTGTCCAAAT TATGGAGGGT CCATTGCTAA GTCCGAAACG TATTGATGAA 
ACTTTGCGAA CCACAAAATT TATGAGACGG CTTTTAGCAT TTTATAAACC CTTTTCCAAC 
AGATTTTCTT CCATACAAAA CACTAAACCA AATCAAAAAT TTATAAAGGT TGGTTGTCTT 
GTTTTCAGGA CTTTACTAGC CAATCCGGAA GGCGTTAAAT ATCTTTCTGA AAGCAAAGTG 
ATTAAACAAA TAGCAGAATC TCTTAGCCAG ATAGACGGAT ATTCAGAACA GGTTTCTGAG 
CCGATTTTCT CGAACAGTAG GCTTCAGAAA ACCCTCACCC ACGGTTACTT TCCTATGTTA 
AAAGTATTGT CAAGCCAAAA GGAAGGTCAT GCAATTATGG AACGATGGAG GATTTTTACT 
ACACTTTACC ATTTGACAGA ATTGAGGAAC AGAGACGATC TCATAATTAT TTTTCTGACT 
AATCTTGATT ATCGGCTAGA GGGGCACACA CGAATTATAT TTTCTAAGGC GCTTAATACC 
GGGCAACAGG CTGTACGCTT AACTGCAACC AAGCATCTTG CGGCTTTAAT CAATTCTGAG 
TCTGCCAATG ACAACTTAAA TCACTGGGCA ATTTCTCTAT TGATATTTCA ACTGTACGAT 
CCTTGCTTGG AAGTATGTAA AACAGCAGTG AAGGTGTTGA ATGAAGTTTG TGCTCGAAAT 
GAAAACTTGC TGGCTCAAGT TGTTCAGCTT CAACCTTCAT TGGCCCATTT GGGTGAAATT 
GGCTCACCTT TACTTTTACG TTTTCTTGCT ACAACTGTCG GATTTCATTA CCTTTCTGAA 
ATTAATTTTA TTGAACATGA ACTCGATAAT TGGTACCATC ATCGAAATAT AGATTACGTG 
GATTTATTGG AGCAAAATTT TTTCCTTTCT TTTGTTTCAA ATCTCAAAAT TATTGACAAG 
AAAAACAATG AACCAGATGA AAATATACTT CCCCTACACT TTTATGGCGA ATTAGTCAAA 
TCTCCACAAG GTTGTGAGGT TCTCGAGTCT TCTGGGCATT TTGAATCTTT TATGGGTACC 
TTGGTGGAAT TTTACGATAA ACCGTTAGGA AATGAAGCCA TCAGGCAGTT AAAGAGTGCT 
CTTTGGGCTA TAGGAAACAT TGGAAAAACC GATCAGGGTA TTACGTTTTT AATTAACCAT 
GATACGATAC CGTTGATTGT CAAATATGCC GAAAATTCAT TGATTCCTAC TGTTCGAGGA 
ACTGCTTATT TTGTATTAGG CTTGATTTCC AGGACTTCTA AAGGCGTTGA GATACTCGAA 
TCACTGCATT GGTATAGCCT TATGTCTTTA ATGGGCACTT CACAAGGAAT TTGCATTCCG 
AGACATGCTG GACAAGTATT ATCAACTCCA AGGAGAAATG TTGAGTTTGT AAATGAGCGC 
GTGCCAACTC CTGAGTTTTC TTCATTACTT AGCTCTTTAA CTAACTCAGA GAGAGAGGTA 
ATTAGGCTCG TTTCCAATTT GTCAAACCAT GTTTTAACAA ACGAGTCAGC ACGGCAACTA 
ACAAAGATTC GTTCGAAAAA TGCTAAAGTT TTCTCCTCTA AACGTTTGGT GAAAGCTTGT 
ATGACCATTC TGGGAAAATT CCATTACCGT GTTCAGATTC AACAATTTGT ATTTGAATTA 
TTTCCTTACT CGGTTTTATT GTCTTCCTCA ACCTCACAGG ACTTAAATGA ATCCCCGTCC 
AGGCCGAATA ATTTGTCAAT TTCCGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ste20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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