ORF » Species Summary » Schizosaccharomyces pombe » tim50 cDNA ORF clone

tim50 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following tim50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tim50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99985 NM_001021478.3
Latest version!		 Schizosaccharomyces pombe putative TIM23 translocase complex subunit Tim50 (tim50), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    tim50 ( NM_001021478.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595583.2 Schizosaccharomyces pombe putative TIM23 translocase complex subunit Tim50 (tim50), mRNA.
    Price & Availability↑
    CloneID OSc99985
    Clone ID Related Accession (Same CDS sequence) NM_001021478.3
    Accession Version NM_001021478.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative TIM23 translocase complex subunit Tim50
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021478.2. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTGAACA GGTCTCTTCT TTTTCGTAAT TTGAGGCTTG CTAGACCTCG TCCTTTTTTG 
    CCCCTTGTTG GCAAACGGTT TGTGACTGAG AAAAGCCAGT CGCAAGAGGA AAAGGACACA 
    TCAAAAATCA CCGAAAACGC AAAAGAAGAA GTTAAACGGG ACACTTCTTC ACTTGCGAAA 
    GAGTCATTAA AATTACTTGA TTTAAATGGT TTGAATGATG AGTCCTACAC TGGAGACCCT 
    GGAAAAGGAC CCGAGTATAC TAGTTCAACG ATGCTAAAGC GAGAAAAGCA GGCTCGTTAT 
    GCATTTTGGG GTTTCTTGGG ATTAACGGGA GGTGGTTTAC TTTACTACGG TCGTCGCTAT 
    GGTCCTGATG AGAAAGAACT AGAAAAGCAG TATCCCGCTG CTGGTTATTC CCCTTCAGAT 
    TGGTGGAATA GGGTAAAGGC CCGTACAAAT AACTTCTTCT CTTACTATCA GGAGCCTGCA 
    TTTGAAAAGT TGCTCCCTGA TCCTCTTCCT GAACCTTATA ATCGACCTTA TACCTTGGTC 
    TTAAGTTTGG ATGATCTTTT GATTCATAGC GAATGGACTC GTCAACATGG TTGGAGAACT 
    GCAAAGCGTC CTGGTTTAGA TTACTTCTTG GGCTATCTCT CCATGTACTA CGAAGTAGTC 
    ATCTTTACTA GACAGTATTT AGCAACTGCC AAGCCTATCA TCGATAAGAT TGATCCATAC 
    CACGTAAGTA TTAGTGCCGT ACTTACTCGC GAGTCAAGCA AATATGAGAA GGGTAAGGTT 
    ATCAAAGATT TATCTTACTT GAATCGTGAC CTGTCTCGAG TGATCATGAT CGATACGAAT 
    CCCGAGTCAT GGTCCAAACA GCCTGATAAC GCAATTGCCA TGGCTCCATG GACAGGAAAT 
    CCTAAGGATA AGGAATTGGT CGGATTAATT CCTCTTTTGG AGTTTATTGC TATCATGGAT 
    ATTAAAGACG TTCGTCCAGT TCTTAAAAGT TATCAAGGTA AAAACATTCC CCTTGAGTAT 
    GCCCGCAGAG AGGAAAAACT ACGCACCAAG TTAATTGAAG ATTGGAATGA GAAGAAGAAG 
    AAAGGCAGTT CCTTTTTGTT TGGAGGACGG AGTGTATCTG AAGAGCCACC CAAATTAATC 
    ATTGATATAC AGCGTGAGCG ACAAAAGGCT GCTTATGCTG AATTTAAAAA GTATATTGAC 
    GAAAATGGAC CTAAAATGCT TGAAGAAGAA AAGGCTCGAG AGGCTGAACA AAAAACAAGC 
    ATCTTTAATC TTCTCTTCCA TCCAGAAGAG GTTCAGCAAC AACAGTTAGA ACAAATGCAG 
    CAACAACAAT TTTCACCCGA AACAAATGCT TCCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595583.2
    CDS88..1446
    Translation

    Target ORF information:

    RefSeq Version NM_001021478.3
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative TIM23 translocase complex subunit Tim50 (tim50), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001021478.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTGAACA GGTCTCTTCT TTTTCGTAAT TTGAGGCTTG CTAGACCTCG TCCTTTTTTG 
    CCCCTTGTTG GCAAACGGTT TGTGACTGAG AAAAGCCAGT CGCAAGAGGA AAAGGACACA 
    TCAAAAATCA CCGAAAACGC AAAAGAAGAA GTTAAACGGG ACACTTCTTC ACTTGCGAAA 
    GAGTCATTAA AATTACTTGA TTTAAATGGT TTGAATGATG AGTCCTACAC TGGAGACCCT 
    GGAAAAGGAC CCGAGTATAC TAGTTCAACG ATGCTAAAGC GAGAAAAGCA GGCTCGTTAT 
    GCATTTTGGG GTTTCTTGGG ATTAACGGGA GGTGGTTTAC TTTACTACGG TCGTCGCTAT 
    GGTCCTGATG AGAAAGAACT AGAAAAGCAG TATCCCGCTG CTGGTTATTC CCCTTCAGAT 
    TGGTGGAATA GGGTAAAGGC CCGTACAAAT AACTTCTTCT CTTACTATCA GGAGCCTGCA 
    TTTGAAAAGT TGCTCCCTGA TCCTCTTCCT GAACCTTATA ATCGACCTTA TACCTTGGTC 
    TTAAGTTTGG ATGATCTTTT GATTCATAGC GAATGGACTC GTCAACATGG TTGGAGAACT 
    GCAAAGCGTC CTGGTTTAGA TTACTTCTTG GGCTATCTCT CCATGTACTA CGAAGTAGTC 
    ATCTTTACTA GACAGTATTT AGCAACTGCC AAGCCTATCA TCGATAAGAT TGATCCATAC 
    CACGTAAGTA TTAGTGCCGT ACTTACTCGC GAGTCAAGCA AATATGAGAA GGGTAAGGTT 
    ATCAAAGATT TATCTTACTT GAATCGTGAC CTGTCTCGAG TGATCATGAT CGATACGAAT 
    CCCGAGTCAT GGTCCAAACA GCCTGATAAC GCAATTGCCA TGGCTCCATG GACAGGAAAT 
    CCTAAGGATA AGGAATTGGT CGGATTAATT CCTCTTTTGG AGTTTATTGC TATCATGGAT 
    ATTAAAGACG TTCGTCCAGT TCTTAAAAGT TATCAAGGTA AAAACATTCC CCTTGAGTAT 
    GCCCGCAGAG AGGAAAAACT ACGCACCAAG TTAATTGAAG ATTGGAATGA GAAGAAGAAG 
    AAAGGCAGTT CCTTTTTGTT TGGAGGACGG AGTGTATCTG AAGAGCCACC CAAATTAATC 
    ATTGATATAC AGCGTGAGCG ACAAAAGGCT GCTTATGCTG AATTTAAAAA GTATATTGAC 
    GAAAATGGAC CTAAAATGCT TGAAGAAGAA AAGGCTCGAG AGGCTGAACA AAAAACAAGC 
    ATCTTTAATC TTCTCTTCCA TCCAGAAGAG GTTCAGCAAC AACAGTTAGA ACAAATGCAG 
    CAACAACAAT TTTCACCCGA AACAAATGCT TCCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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