myo1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo1
Entrez Gene ID	2539659
Full Name	myosin type I
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021309.2	NP_595402.1	myosin I myo1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYO1F	NP_001179677.1
Gallus gallus (chicken)	MYO1F	NP_990585.1
Saccharomyces cerevisiae (baker's yeast)	MYO5	NP_013827.1
Kluyveromyces lactis	KLLA0B12562g	XP_452090.1
Homo sapiens (human)	MYO1F	NP_036467.2
Rattus norvegicus (Norway rat)	Myo1f	NP_001101546.1
Xenopus tropicalis (tropical clawed frog)	myo1f	XP_002938604.1
Mus musculus (house mouse)	Myo1f	NP_444444.2
Schizosaccharomyces pombe (fission yeast)	myo1	NP_595402.1
Pan troglodytes (chimpanzee)	MYO1F	XP_512345.3
Saccharomyces cerevisiae (baker's yeast)	MYO3	NP_012793.2
Eremothecium gossypii	AGOS_AEL306C	NP_984554.2
Magnaporthe oryzae (rice blast fungus)	MGG_00748	XP_003718252.1
Canis lupus familiaris (dog)	MYO1F	XP_005633017.1
Danio rerio (zebrafish)	myo1f	NP_001243600.1
Neurospora crassa	NCU02111	XP_964105.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Yeast strains expressing mutant Myo1 exhibited defects in growth and endocytosis similar to those observed in the myo1 deletion strain.
Title: Effects of FSGS-associated mutations on the stability and function of myosin-1 in fission yeast.

proteins encoded by the Myo1-associated mRNAs immunoprecipitated each other's transcripts, but not myosin mRNAs
Title: Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites.

Phosphorylation of the Myo1 TEDS site by Pak1 and Nak1 plays a crucial role in regulating Myo1 localisation and cellular function.
Title: Ste20-kinase-dependent TEDS-site phosphorylation modulates the dynamic localisation and endocytic function of the fission yeast class I myosin, Myo1.

These results suggest a close relationship between Cam2 and Myo1. In addition, both F-actin and Myo1 localized with Cam2 in the leading edge region.
Title: Localization of type I myosin and F-actin to the leading edge region of the forespore membrane in Schizosaccharomyces pombe.

myo1p, is required for proper organization of distinct sterol-rich membrane domains.
Title: Role of fission yeast myosin I in organization of sterol-rich membrane domains.

The following myo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101362	NM_001021309.2 Latest version!	Schizosaccharomyces pombe myosin I myo1 (myo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo1 ( NM_001021309.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595402.1 Schizosaccharomyces pombe myosin I myo1 (myo1), mRNA.

Price & Availability↑

CloneID	OSc101362
Clone ID Related Accession (Same CDS sequence)	NM_001021309.2
Accession Version	NM_001021309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	myosin I myo1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021309.1.

ATGGCCATCC TTAAGAGAAC AAACCGCGCG AAAGCTGCGA CTGCAGCTGC TCCCAATAGC 
ACTGGGAAAA GCAATGGTAT TAAAAAGGCT GTTTATACCA GCACACGCAA AAAGACTGTG 
GGTGTTGATG ATTTAACCCT ACTTTCGAAA ATTACAGATG AGGAAATTAA CAAAAACTTG 
GAACTGAGAT TTCGCAATGG AGAGATTTAT ACATATATTG GACATGTGTT GATTTCTGTA 
AATCCATTTC GAGACCTGGG TATTTATACG ATGGATATTT TGAAATCATA TCAAGGAAAA 
AACCGTTTGG AAACGAGCCC TCACGTTTAT GCAATTGCTG AGAACGCTTA CTATCAAATG 
AAGTCTTATC ATGAGAATCA ATGTATTATC ATTTCTGGTG AATCGGGTGC TGGTAAGACT 
GAGGCTGCAA AAAGGATCAT GCAATATATT ACACATGTTT CAAAGTCCGT TGGCACTGAA 
ATTGAAAGAG TTTCCGAAAT TATTTTAGCT ACCAATCCTT TATTGGAAAG TTTTGGTTGT 
GCAAAAACTC TTCGCAATAA TAATTCTTCT CGTCATGGAA AGTATTTAGA AATGATTTTT 
AATTCCGGAG GTGTTCCTGT CGGCGCTAAA ATTACGAACT ACTTATTAGA AAAGAACCGA 
ATTGTTAATC AAGTTCGCAA TGAGCGTAAT TTCCATATTT TCTATCAATT CACCAAGAGC 
GCTCCTCAGA AATATCGTGA TACATATGGC ATTCAAGGTC CGGAGAATTA TGTTTACACT 
TCTGCTTGTC AATGTTTGTC GGTTGATGGG ATTTCAGATG AGAAGGACTT CCAAGGAACG 
ATGAACGCCA TGAAGGTTAT TGGTATCACC GAGCCGGAGC AAGATGAAAT ATTTAGGATG 
CTTTCTATCA TCCTTTGGTT AGGAAATATT CAATTTCAAG AAGGCCAAGA TGGTGGTAGC 
GTGATTTCTG ACAAGAGCAT CACCGAATTT TTAGGCTATT TGATCGGTGT TCCTGTTGCG 
GCTATTGAGC GTGCCTTGAC AATTCGTATT ATGCAAACTC AGCATGGTGC AAGGCGCGGA 
TCTGTATATG AAGTCCCCTT AAATCCAACA CAAGCTTTGG CTGTTCGTGA TGCTTTGTCT 
ATGGCGATTT ATAACTGTCT TTTTGATTGG ATTGTCGAAC GTGTCAACAA AGCTTTGGTC 
ACATCAGACA ACAGCGTTTC CAATTCGATT GGTATTTTAG ATATTTACGG CTTTGAAATC 
TTTGAAAACA ACTCTTTTGA ACAATTGTGC ATTAACTATG TTAATGAAAA ATTGCAACAA 
ATTTTTATAG AACTTACTTT GAAAACTGAA CAGGAAGAAT ACGTGCGTGA GCAAATTGCT 
TGGACTCCTA TCAAATATTT CAACAATAAA GTTGTATGTG ATTTGATTGA ATCCAAACGA 
CCTCCTGGTC TTTTCGCAGC AATGAACGAC GCTATCGCTA CCGCTCATGC TGACTCTGCT 
GCGGCGGATA GTGCTTTTGC TCAGCGGTTA AACTTTTTAT CCTCCAATCC ACATTTTGAA 
CAGAGACAAA ATCAATTTAT AGTCAAACAT TACGCTGGTG ATGTAACCTA CAGCATTACA 
GGAATGACCG ATAAGAATAA GGATCAGTTA GCAACTGATA TTCTTAACCT TATTCATTCT 
TCCAACAACG AGTTCATGAA ATCTATTTTC CCAGTGGCTG AAGAGAGTAA CTCCCGTCGT 
CGTCCTCCTA CAGCTGGTGA TCGTATCAAA ACTTCTGCTA ATGATTTAGT AGAGACTCTT 
ATGAAATGTC AACCATCGTA TATACGTACG ATTAAACCCA ATCAGACTAA GTCGCCTAAC 
GATTACGATC AGCAGATGGT TTTACATCAA ATCAAATATT TAGGTTTGCA AGAAAACATT 
CGTATTCGTC GTGCTGGTTT TGCTTATCGT CAAGCTTTCG ATACGTTTGC TCAGCGTTTT 
GCAGTTCTTA GTGGTAAAAC CAGTTATGCA GGAGAATACA CTTGGCAGGG TGATGATAAG 
TCTGCTTGCG AACAGATTTT GAAAGATACC AATATTCCTT CATCTGAATA CCAAATGGGT 
ACTAGCAAAG TTTTCATTAA AAATCCGGAG ACCTTATTCG CGCTCGAAGA CATGCGTGAT 
AAGTTTTGGG ATACAATGGC AACCCGTATA CAACGTGCTT GGCGTTCTTA TGTTAGAAGA 
AGATCTGAAG CTGCTGCTTG TATTCAGAAG TTGTGGAATA GGAACAAAGT TAACATGGAA 
CTTGAACGTG TTCGTAATGA GGGTACTAAA CTTTTGCAAG GTAAAAAGCA ACGCAGAAGA 
TATAGTATTT TGGGATCCCG TAAGTTTTAT GGAGATTATC TATCAGCTTC AAAGCCTAAT 
GGTACTCTAT GGAACACATG CGGCCTCTCT CAAAACGATC ACGTAATTTT CTCAATGAGA 
TGTGAGGTCC TTGTTCATAA GCTAGGCCGC ACTTCCAAAC CTTCTCCTCG TCAACTGGTT 
CTTACCAAAA AGAATTTATA TTTGGTGATT ACAAAAATTG TTGACCAAAA GCTCACACAA 
CAGGTGGAGA AGAAGTTCGC TGTATCTTCC ATCGATTCTG TTGGTCTTAC AAATTTGCAA 
GATGATTGGG TTGCAATTCG TAATAAGTCC AGCCAAAATG GAGATATGTT TTTGCGATGC 
TTTTTCAAAA CAGAATTTAT AACTACCTTA AAGAGAATCA ATCGTAATAT TCAAGTTATC 
GTGGGTCCCA CCATTCAATA CTGCCGAAAG CCTGGCAAGG TACAAACTGT TAAGACGGCA 
AAAGACGAAA CCACTAAGGA TTATGACTAC TACAAGAGTG GAACTATTCA TGTTGGTACT 
GGCTTGCCTC CTACCAGTAA GAGTAAGCCT TTCCCTCGCT TAGCAACTGG TGGTAGTACA 
GCAGCTGCTA GAGGGCCTCG CCCAGTTGTG CAGAACAAAC CTGCTGCTAC AAAGCCCGTA 
TCTATGCCGG CTGCGAAGAG CAAACCTGCT CCTATGGCTA ATCCCGTAAG TACTGCTCAA 
CAAACCCAAA ATCGTCCCCC TGCTCCTGCT ATGCAAGCTC GTCCTAATAC AACTCAAGCT 
GCAGCCCCAG TTACAAGTAC GACTACAACT ATTAAGCAAG CAACCACTGT TTCTGCTTCT 
AAACCAGCTC CTTCCACGGT AACTTCTGCA GCTTCTTCTC CAAGCAATAT CTCTAAACCC 
TCTGCTCCAG TTGCTAATAA CGTCAGCAAG CCTAGTGCTG TTCCTCCTCC TCCTCCTCCT 
CCTCCCGCTG AAGTCGAAAA GAAAGACTTG TACTTGGCCT TATATGACTT TGCGGGACGC 
AGTCCAAATG AAATGACTAT CAAGAAAGAC GAAATTATAG AAATTGTTCA AAAGGAGCCA 
AGCGGGTGGT GGCTTGCTTT AAAGAATGGT GCTGAAGGAT GGGTTCCCGC AACCTATGTG 
ACAGAATACA AAGGTTCTAC ACCTCAAACC ACCGCTTCTT CGACTAATGT TGCAGCTCAA 
GCGAATAATA ATGCTAGTCC AGCTGAAGTC AACAACTTAG CTGGTTCACT TGCTGATGCC 
TTGCGCATGC GAGCATCAGC TGTGCGTGGT AGTGATGAAG AAGAAGATTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595402.1
CDS	263..3916
Translation	MAILKRTNRAKAATAAAPNSTGKSNGIKKAVYTSTRKKTVGVDDLTLLSKITDEEINKNLELRFRNGEIYTYIGHVLISVNPFRDLGIYTMDILKSYQGKNRLETSPHVYAIAENAYYQMKSYHENQCIIISGESGAGKTEAAKRIMQYITHVSKSVGTEIERVSEIILATNPLLESFGCAKTLRNNNSSRHGKYLEMIFNSGGVPVGAKITNYLLEKNRIVNQVRNERNFHIFYQFTKSAPQKYRDTYGIQGPENYVYTSACQCLSVDGISDEKDFQGTMNAMKVIGITEPEQDEIFRMLSIILWLGNIQFQEGQDGGSVISDKSITEFLGYLIGVPVAAIERALTIRIMQTQHGARRGSVYEVPLNPTQALAVRDALSMAIYNCLFDWIVERVNKALVTSDNSVSNSIGILDIYGFEIFENNSFEQLCINYVNEKLQQIFIELTLKTEQEEYVREQIAWTPIKYFNNKVVCDLIESKRPPGLFAAMNDAIATAHADSAAADSAFAQRLNFLSSNPHFEQRQNQFIVKHYAGDVTYSITGMTDKNKDQLATDILNLIHSSNNEFMKSIFPVAEESNSRRRPPTAGDRIKTSANDLVETLMKCQPSYIRTIKPNQTKSPNDYDQQMVLHQIKYLGLQENIRIRRAGFAYRQAFDTFAQRFAVLSGKTSYAGEYTWQGDDKSACEQILKDTNIPSSEYQMGTSKVFIKNPETLFALEDMRDKFWDTMATRIQRAWRSYVRRRSEAAACIQKLWNRNKVNMELERVRNEGTKLLQGKKQRRRYSILGSRKFYGDYLSASKPNGTLWNTCGLSQNDHVIFSMRCEVLVHKLGRTSKPSPRQLVLTKKNLYLVITKIVDQKLTQQVEKKFAVSSIDSVGLTNLQDDWVAIRNKSSQNGDMFLRCFFKTEFITTLKRINRNIQVIVGPTIQYCRKPGKVQTVKTAKDETTKDYDYYKSGTIHVGTGLPPTSKSKPFPRLATGGSTAAARGPRPVVQNKPAATKPVSMPAAKSKPAPMANPVSTAQQTQNRPPAPAMQARPNTTQAAAPVTSTTTTIKQATTVSASKPAPSTVTSAASSPSNISKPSAPVANNVSKPSAVPPPPPPPPAEVEKKDLYLALYDFAGRSPNEMTIKKDEIIEIVQKEPSGWWLALKNGAEGWVPATYVTEYKGSTPQTTASSTNVAAQANNNASPAEVNNLAGSLADALRMRASAVRGSDEEEDW

Target ORF information:

RefSeq Version	NM_001021309.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe myosin I myo1 (myo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021309.2

ORF Insert Sequence:

ATGGCCATCC TTAAGAGAAC AAACCGCGCG AAAGCTGCGA CTGCAGCTGC TCCCAATAGC 
ACTGGGAAAA GCAATGGTAT TAAAAAGGCT GTTTATACCA GCACACGCAA AAAGACTGTG 
GGTGTTGATG ATTTAACCCT ACTTTCGAAA ATTACAGATG AGGAAATTAA CAAAAACTTG 
GAACTGAGAT TTCGCAATGG AGAGATTTAT ACATATATTG GACATGTGTT GATTTCTGTA 
AATCCATTTC GAGACCTGGG TATTTATACG ATGGATATTT TGAAATCATA TCAAGGAAAA 
AACCGTTTGG AAACGAGCCC TCACGTTTAT GCAATTGCTG AGAACGCTTA CTATCAAATG 
AAGTCTTATC ATGAGAATCA ATGTATTATC ATTTCTGGTG AATCGGGTGC TGGTAAGACT 
GAGGCTGCAA AAAGGATCAT GCAATATATT ACACATGTTT CAAAGTCCGT TGGCACTGAA 
ATTGAAAGAG TTTCCGAAAT TATTTTAGCT ACCAATCCTT TATTGGAAAG TTTTGGTTGT 
GCAAAAACTC TTCGCAATAA TAATTCTTCT CGTCATGGAA AGTATTTAGA AATGATTTTT 
AATTCCGGAG GTGTTCCTGT CGGCGCTAAA ATTACGAACT ACTTATTAGA AAAGAACCGA 
ATTGTTAATC AAGTTCGCAA TGAGCGTAAT TTCCATATTT TCTATCAATT CACCAAGAGC 
GCTCCTCAGA AATATCGTGA TACATATGGC ATTCAAGGTC CGGAGAATTA TGTTTACACT 
TCTGCTTGTC AATGTTTGTC GGTTGATGGG ATTTCAGATG AGAAGGACTT CCAAGGAACG 
ATGAACGCCA TGAAGGTTAT TGGTATCACC GAGCCGGAGC AAGATGAAAT ATTTAGGATG 
CTTTCTATCA TCCTTTGGTT AGGAAATATT CAATTTCAAG AAGGCCAAGA TGGTGGTAGC 
GTGATTTCTG ACAAGAGCAT CACCGAATTT TTAGGCTATT TGATCGGTGT TCCTGTTGCG 
GCTATTGAGC GTGCCTTGAC AATTCGTATT ATGCAAACTC AGCATGGTGC AAGGCGCGGA 
TCTGTATATG AAGTCCCCTT AAATCCAACA CAAGCTTTGG CTGTTCGTGA TGCTTTGTCT 
ATGGCGATTT ATAACTGTCT TTTTGATTGG ATTGTCGAAC GTGTCAACAA AGCTTTGGTC 
ACATCAGACA ACAGCGTTTC CAATTCGATT GGTATTTTAG ATATTTACGG CTTTGAAATC 
TTTGAAAACA ACTCTTTTGA ACAATTGTGC ATTAACTATG TTAATGAAAA ATTGCAACAA 
ATTTTTATAG AACTTACTTT GAAAACTGAA CAGGAAGAAT ACGTGCGTGA GCAAATTGCT 
TGGACTCCTA TCAAATATTT CAACAATAAA GTTGTATGTG ATTTGATTGA ATCCAAACGA 
CCTCCTGGTC TTTTCGCAGC AATGAACGAC GCTATCGCTA CCGCTCATGC TGACTCTGCT 
GCGGCGGATA GTGCTTTTGC TCAGCGGTTA AACTTTTTAT CCTCCAATCC ACATTTTGAA 
CAGAGACAAA ATCAATTTAT AGTCAAACAT TACGCTGGTG ATGTAACCTA CAGCATTACA 
GGAATGACCG ATAAGAATAA GGATCAGTTA GCAACTGATA TTCTTAACCT TATTCATTCT 
TCCAACAACG AGTTCATGAA ATCTATTTTC CCAGTGGCTG AAGAGAGTAA CTCCCGTCGT 
CGTCCTCCTA CAGCTGGTGA TCGTATCAAA ACTTCTGCTA ATGATTTAGT AGAGACTCTT 
ATGAAATGTC AACCATCGTA TATACGTACG ATTAAACCCA ATCAGACTAA GTCGCCTAAC 
GATTACGATC AGCAGATGGT TTTACATCAA ATCAAATATT TAGGTTTGCA AGAAAACATT 
CGTATTCGTC GTGCTGGTTT TGCTTATCGT CAAGCTTTCG ATACGTTTGC TCAGCGTTTT 
GCAGTTCTTA GTGGTAAAAC CAGTTATGCA GGAGAATACA CTTGGCAGGG TGATGATAAG 
TCTGCTTGCG AACAGATTTT GAAAGATACC AATATTCCTT CATCTGAATA CCAAATGGGT 
ACTAGCAAAG TTTTCATTAA AAATCCGGAG ACCTTATTCG CGCTCGAAGA CATGCGTGAT 
AAGTTTTGGG ATACAATGGC AACCCGTATA CAACGTGCTT GGCGTTCTTA TGTTAGAAGA 
AGATCTGAAG CTGCTGCTTG TATTCAGAAG TTGTGGAATA GGAACAAAGT TAACATGGAA 
CTTGAACGTG TTCGTAATGA GGGTACTAAA CTTTTGCAAG GTAAAAAGCA ACGCAGAAGA 
TATAGTATTT TGGGATCCCG TAAGTTTTAT GGAGATTATC TATCAGCTTC AAAGCCTAAT 
GGTACTCTAT GGAACACATG CGGCCTCTCT CAAAACGATC ACGTAATTTT CTCAATGAGA 
TGTGAGGTCC TTGTTCATAA GCTAGGCCGC ACTTCCAAAC CTTCTCCTCG TCAACTGGTT 
CTTACCAAAA AGAATTTATA TTTGGTGATT ACAAAAATTG TTGACCAAAA GCTCACACAA 
CAGGTGGAGA AGAAGTTCGC TGTATCTTCC ATCGATTCTG TTGGTCTTAC AAATTTGCAA 
GATGATTGGG TTGCAATTCG TAATAAGTCC AGCCAAAATG GAGATATGTT TTTGCGATGC 
TTTTTCAAAA CAGAATTTAT AACTACCTTA AAGAGAATCA ATCGTAATAT TCAAGTTATC 
GTGGGTCCCA CCATTCAATA CTGCCGAAAG CCTGGCAAGG TACAAACTGT TAAGACGGCA 
AAAGACGAAA CCACTAAGGA TTATGACTAC TACAAGAGTG GAACTATTCA TGTTGGTACT 
GGCTTGCCTC CTACCAGTAA GAGTAAGCCT TTCCCTCGCT TAGCAACTGG TGGTAGTACA 
GCAGCTGCTA GAGGGCCTCG CCCAGTTGTG CAGAACAAAC CTGCTGCTAC AAAGCCCGTA 
TCTATGCCGG CTGCGAAGAG CAAACCTGCT CCTATGGCTA ATCCCGTAAG TACTGCTCAA 
CAAACCCAAA ATCGTCCCCC TGCTCCTGCT ATGCAAGCTC GTCCTAATAC AACTCAAGCT 
GCAGCCCCAG TTACAAGTAC GACTACAACT ATTAAGCAAG CAACCACTGT TTCTGCTTCT 
AAACCAGCTC CTTCCACGGT AACTTCTGCA GCTTCTTCTC CAAGCAATAT CTCTAAACCC 
TCTGCTCCAG TTGCTAATAA CGTCAGCAAG CCTAGTGCTG TTCCTCCTCC TCCTCCTCCT 
CCTCCCGCTG AAGTCGAAAA GAAAGACTTG TACTTGGCCT TATATGACTT TGCGGGACGC 
AGTCCAAATG AAATGACTAT CAAGAAAGAC GAAATTATAG AAATTGTTCA AAAGGAGCCA 
AGCGGGTGGT GGCTTGCTTT AAAGAATGGT GCTGAAGGAT GGGTTCCCGC AACCTATGTG 
ACAGAATACA AAGGTTCTAC ACCTCAAACC ACCGCTTCTT CGACTAATGT TGCAGCTCAA 
GCGAATAATA ATGCTAGTCC AGCTGAAGTC AACAACTTAG CTGGTTCACT TGCTGATGCC 
TTGCGCATGC GAGCATCAGC TGTGCGTGGT AGTGATGAAG AAGAAGATTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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myo1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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