rgf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rgf1
Entrez Gene ID	2539580
Full Name	RhoGEF for Rho1, Rgf1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023106.2	NP_588116.1	RhoGEF family guanine nucleotide exchange factor Rgf1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR585W	NP_986132.2
Schizosaccharomyces pombe (fission yeast)	rgf1	NP_588116.1
Magnaporthe oryzae (rice blast fungus)	MGG_03064	XP_003720612.1
Kluyveromyces lactis	KLLA0B07799g	XP_451877.1
Saccharomyces cerevisiae (baker's yeast)	ROM2	NP_013475.1
Neurospora crassa	NCU00668	XP_965808.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rgf1p and Rgf2p are alternative guanine nucleotide exchange factors with an essential overlapping function in Rho1p activation during vegetative growth.
Title: Fission yeast rgf2p is a rho1p guanine nucleotide exchange factor required for spore wall maturation and for the maintenance of cell integrity in the absence of rgf1p.

Results show that Rgf1p, a Rho1p-specific GEF, acts upstream from the Pck2-Pmk1p MAPK cell integrity signaling pathway.
Title: The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.

Rgf1p probably activates the Rho1 functions necessary for coordinating actin deposition with cell wall biosynthesis during bipolar growth, allowing the cells to remodel their wall without risk of rupture.
Title: Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.

Rgf1 is involved in cell wall and septum formation.
Title: Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.

The following rgf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rgf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99698	NM_001023106.2 Latest version!	Schizosaccharomyces pombe RhoGEF family guanine nucleotide exchange factor Rgf1 (rgf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rgf1 ( NM_001023106.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588116.1 Schizosaccharomyces pombe RhoGEF family guanine nucleotide exchange factor Rgf1 (rgf1), mRNA.

Price & Availability↑

CloneID	OSc99698
Clone ID Related Accession (Same CDS sequence)	NM_001023106.2
Accession Version	NM_001023106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RhoGEF family guanine nucleotide exchange factor Rgf1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023106.1.

ATGGATTACC GGCATCCAAA TGCACTCGGT GTGAATGAGA GCTCGCGTGC TTATGAGGAA 
ATTTTTGGTG CTCCAAGAAA ACGGGAGCCG GCGAGAACTG TAAGTACTCC TGCGTTCATG 
GAACCGGCTC CAGTCTCAAA AAAACCTCTT CCTCCTCCCA CGCGAAGGCT TCCTCGGAAA 
CCTTTGCCCT TTCGTTCCAC GAGTTTGCAG CCTCCATCGT CTCAACCTCC TGCACCTCCA 
ACTCATCAGA GAGAAGCATC TCCTGTGAAA AATATTGAGC ACAGTGAATC TTTTCCTTCC 
GTTTTTGGTA CTTCAAATAA TCACCAGATC GTCCCATTGA CTCTTAAAGA CGGAAATGAT 
TTTGGTGCTC TATATGCATC TTTAAACACC ACACCCCATT TTCCTCAGGT ATCGAATCAT 
GCGCCCAATA ACTCTAATTC CCCTTCTTTG ACTTGGCACA CATCATCCGG TGATGATTCC 
AACCAAAATC CTTTTTTCGT TCGGCGTCAG TCTCAGAGTA GCACGTCTCC GGTTTCGGAT 
TCAGTAGATG AGAACCTCCT TTCAGCAGTC TCTTCTGTTA CAGAGTCCGT AGAAACAAAC 
CTTCATCTCG ATCAAAATTA TCCTTACGGC TCTCCTGTTC GTTCGTCTAA AAATCCCTTT 
CTCTCTTCAA ATTCTCGATT ACCAACTGAT GATTCTTCTC ACACTGTTGG TTCTCATTCA 
TTTACTTCAG GAACTCACCC ACCCATTGTT TCTTCTAATT CCGCCTTTAC ACTTCCCAAT 
GCTGTTACTC CTGCAGCTCA AGCACCTCTC ATACGTAGTG TTAGTGAATA TCCTGCTAAC 
GTTTCTCCAC CTGCCCAGTC CCTTCAGCTA CCAAAATCTA CTAGCAATCC CGCGGATCTT 
CATTTAAGCA TAGCTTCTGC TAGCAGCCAT AAGAATATTT TTTCTGGGTT GGACGTCTTT 
TCTAATGTCT TTCATGGTCC CTCTACCACA TTACGAGACA GAGAACATGA TATGCGAAAT 
CGCTCATTTG ATCATTCTAC CTTGGCTCAT TATGAAGCAG TTAAGCAGCA GAGACTTGGT 
GTTGAACCTA CTGCAAGGTC CTTTACGTTG AGCTCTTATA AGTCCCGTGC AAGTGGAAAT 
TCCCTAATTA ATGATCGAAG CTCTACAACA ACGCCGACGT TTGTTAATTC GGAAGCTAGC 
TCGCCTGTTC ATAAAAATAA ACGAAGACGC AGAATATATG CTGCCTTACT TTCTCGTGTT 
GCCAGCGAAT TGTTAGATAG ACTACAGCTA GGTGATATCA CGAAAGACGG TTTAATTTAT 
TCTAATGCCT TTACTGGCGA TCATGCAGTC ACCGTGCTAA TGGGTATTAT ACATACTTCT 
GATAGAAATC TCGCTTTACT TGTCGGACGC TCTTTAGATG CACAAAAATT TATTCATGAT 
GTTACCTATG ATCATCGTCT TCGTGATTCT CATCGTGAAA TATATCAGCT TCAAGGTACT 
GGTTATCGCC CCTTCTTACG CGCAAATGAT AATGCATCCA TTAACAACAA AAACCATCAT 
AAGGAATTGG AGGACAATGA GTCGGGCACT CGAATCTCAC CGTCCACGTT AGGTGACACT 
TCTTTTCCAA ATGGTATATT TACTTTATTG ACGCATTGTT ACTCTCCAAC ATGTGCAAAA 
GATCATCCCT GCTATTCTAT TTCATGTCCT CGACGATTGG AACAGCAGCA CAGACTATTT 
GCCAAAATGC GTGCAAACAC TGAACAGTCT TCCTCCCTAG CGTTTGATGA TAAGGAACAA 
AAATTGTGGA TTCACAGCGT ACCCCAGGAG ATTGCTTATA GTGTCAGTGA CCGGGAAAGA 
AAGCGTCAAG AAGTAATTTG TGAGGTTATC TATACTGAGC GAGATTTTGT TAAGGATTTG 
GAATATCTTC GGGATTATTG GATTAAACCA CTATGGGCTT CATCTTGTAT ACCTGAACGA 
AAAAAAGAAA AATTTATTCG TACTGTGTTT TTAAATGCTT TAGAGGTTCA AGCAGTTAAT 
TCTAAATTGG CTGAAGCTCT TACTAAAAGA CAAAACTATA AACCCATTGT GGATAATATT 
GCGGATATAT TTTTGGAGCA CGTTCCAAAA TTTGAGCCTT TCATACGATA TGGTGCAGGC 
CAACTCTACG GAAAATACGA GTTTGAGAAG GAAAAGTCAT CTAATCCTGC ATTTGCTAAG 
TTTGTCAGTG ACGTTGAACG TCTGAAAGAA TCACGAAAGC TAGAGCTAAA CGGCTATCTG 
ACTAAACCAA CTACCAGATT GGCTCGTTAT CCATTACTTC TAGAAGCGGT ATTAAAATAT 
ACTGATGAGG GTAACCCTGA TAAACAAGAC ATTCCGAAGG TCATTAATAT CGTTAGAGGA 
TTTCTCTCAC GTCTTAATGT TGAAAGCGGA AAAGCAGAAA ATAAGTTTAA CTTGTTTCAT 
TTGAATCAAC AGCTGGTCTT TAAACCTGGA GAACATTATG ATTTACATCT GCTTGATGCG 
AACAGACAAT TAATATTCAA AGGACCCTTG AAGAAAAGAA GTGCTGGTTC CACTTCTTCA 
GAGTCTGCGT CGGATGTTAC TTTGTTTCTC TTTGACCATG CTTTGTTGAT AGTAAAACCA 
AAGACTATTA ATAAACGTGA ATTATTGAAA GTTTTTCAAA GACCTATTCC TCTATTACTT 
CTTCAACTAT TCCTTGTTGA TGATAATGGA TTAAGAATCC CTTATTCAAG TAAGCAACAA 
CTAGCAGCTG TATCAAAAGC AGCGAATGGG AAGCCACCTT CTAGATTTTA TCCATTTAGC 
TTACAACTTT TGGGTCGTAG AGGGTACGAA ATCACTCTTT ATGCAACCAC TGAAGTTAGT 
AGGGATAAGT GGTTAGAGCA TATTGACAAC CAACAAACAT TGCTACAACA TCGTAACCAG 
TGGTTTGAGT CTGTTACCAT TTGCTCTAAT TTCTTTGTTG GTGATAATAA AGTAAATGCG 
ATAGGCGTTT ATGATAGTGG TCGTCGACTT TTATATGGGA CTGACACAGG TGTGTACGTC 
TCTCTTCGAA AAGCAAACAG CCCACTTCAA TTCAAGCCTG TACGTGCGTT AAATATTCCC 
AACATTTCAC AGCTTGAGGT TATTGAAGAG TATAGTTTAT TGCTACTGCT TTCCGATAAG 
GTGTTATATT CGTATCCTTT GGAAATGATT GATGCGGATA CTACTCAAGC CCCTAAAAAA 
GCAAGGAAAG TTTCTGGTCA TACAACTTTC TTTCGCGTAG GAATTTGTCT GGGTAAAGTT 
CTTGTGTGTG CTGTCAAAAG TTCCGTCTTG AGTGCTACTA TAAAGGTTTT TGAACCAGTC 
ACTAATTACT CTAAAACACG GAACATGCCA AGCTTGAAAA AATTCCTTAC TGTTAACCAA 
GACCCATTGA GGATTGTCAA AGAGCTTTAT ATACCCACGG AATCAACTTC GGTTCACTTC 
CTGAAGAATA AACTCTGTGT CGGATGTACG AGAGGTTTTG AGGTGGTCAG TCTCGATAAC 
CTAGAGACGC AGTCTCTACT GGATCCAGCC GATACGTCTC TTGAATTTGT GGAGAAGAAA 
GAAAATGTTA AACCTATCGC TATCTATCGT ATGAATGGCG GTGAATTTCT TTTATGCTAT 
TCCCAATTTG CTTTTTACGT AAATCGAGAT GGATGGCGTT CTCGTCCTAC ATGGTTTGTT 
GTTTGGGAAG GAAGTCCTCA AAACTTTGCC TTAAGTTATC CTTATATATT GGCTTTTGAA 
CCCACTTTTA TTGAAATCAG ACATGTTGAA ACCTCTGAAT TGATCCATGT CATTTCCGGC 
AGAAACATCC GTTTGCTTGC TGATGGAAGA GGAAAGTTAG GGGACGGTGG TGAAATATTT 
TATGCGTGCG ATCAACGTGG TGAAAATTGT GAGACAAGTG TTGTTTGTTC CCTTCGATTG 
ACGAGTGCTG CTGCCCACGC CAAAGAGCAG CATGTAGACA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588116.1
CDS	53..4057
Translation	MDYRHPNALGVNESSRAYEEIFGAPRKREPARTVSTPAFMEPAPVSKKPLPPPTRRLPRKPLPFRSTSLQPPSSQPPAPPTHQREASPVKNIEHSESFPSVFGTSNNHQIVPLTLKDGNDFGALYASLNTTPHFPQVSNHAPNNSNSPSLTWHTSSGDDSNQNPFFVRRQSQSSTSPVSDSVDENLLSAVSSVTESVETNLHLDQNYPYGSPVRSSKNPFLSSNSRLPTDDSSHTVGSHSFTSGTHPPIVSSNSAFTLPNAVTPAAQAPLIRSVSEYPANVSPPAQSLQLPKSTSNPADLHLSIASASSHKNIFSGLDVFSNVFHGPSTTLRDREHDMRNRSFDHSTLAHYEAVKQQRLGVEPTARSFTLSSYKSRASGNSLINDRSSTTTPTFVNSEASSPVHKNKRRRRIYAALLSRVASELLDRLQLGDITKDGLIYSNAFTGDHAVTVLMGIIHTSDRNLALLVGRSLDAQKFIHDVTYDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELEDNESGTRISPSTLGDTSFPNGIFTLLTHCYSPTCAKDHPCYSISCPRRLEQQHRLFAKMRANTEQSSSLAFDDKEQKLWIHSVPQEIAYSVSDRERKRQEVICEVIYTERDFVKDLEYLRDYWIKPLWASSCIPERKKEKFIRTVFLNALEVQAVNSKLAEALTKRQNYKPIVDNIADIFLEHVPKFEPFIRYGAGQLYGKYEFEKEKSSNPAFAKFVSDVERLKESRKLELNGYLTKPTTRLARYPLLLEAVLKYTDEGNPDKQDIPKVINIVRGFLSRLNVESGKAENKFNLFHLNQQLVFKPGEHYDLHLLDANRQLIFKGPLKKRSAGSTSSESASDVTLFLFDHALLIVKPKTINKRELLKVFQRPIPLLLLQLFLVDDNGLRIPYSSKQQLAAVSKAANGKPPSRFYPFSLQLLGRRGYEITLYATTEVSRDKWLEHIDNQQTLLQHRNQWFESVTICSNFFVGDNKVNAIGVYDSGRRLLYGTDTGVYVSLRKANSPLQFKPVRALNIPNISQLEVIEEYSLLLLLSDKVLYSYPLEMIDADTTQAPKKARKVSGHTTFFRVGICLGKVLVCAVKSSVLSATIKVFEPVTNYSKTRNMPSLKKFLTVNQDPLRIVKELYIPTESTSVHFLKNKLCVGCTRGFEVVSLDNLETQSLLDPADTSLEFVEKKENVKPIAIYRMNGGEFLLCYSQFAFYVNRDGWRSRPTWFVVWEGSPQNFALSYPYILAFEPTFIEIRHVETSELIHVISGRNIRLLADGRGKLGDGGEIFYACDQRGENCETSVVCSLRLTSAAAHAKEQHVDK

Target ORF information:

RefSeq Version	NM_001023106.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RhoGEF family guanine nucleotide exchange factor Rgf1 (rgf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023106.2

ORF Insert Sequence:

ATGGATTACC GGCATCCAAA TGCACTCGGT GTGAATGAGA GCTCGCGTGC TTATGAGGAA 
ATTTTTGGTG CTCCAAGAAA ACGGGAGCCG GCGAGAACTG TAAGTACTCC TGCGTTCATG 
GAACCGGCTC CAGTCTCAAA AAAACCTCTT CCTCCTCCCA CGCGAAGGCT TCCTCGGAAA 
CCTTTGCCCT TTCGTTCCAC GAGTTTGCAG CCTCCATCGT CTCAACCTCC TGCACCTCCA 
ACTCATCAGA GAGAAGCATC TCCTGTGAAA AATATTGAGC ACAGTGAATC TTTTCCTTCC 
GTTTTTGGTA CTTCAAATAA TCACCAGATC GTCCCATTGA CTCTTAAAGA CGGAAATGAT 
TTTGGTGCTC TATATGCATC TTTAAACACC ACACCCCATT TTCCTCAGGT ATCGAATCAT 
GCGCCCAATA ACTCTAATTC CCCTTCTTTG ACTTGGCACA CATCATCCGG TGATGATTCC 
AACCAAAATC CTTTTTTCGT TCGGCGTCAG TCTCAGAGTA GCACGTCTCC GGTTTCGGAT 
TCAGTAGATG AGAACCTCCT TTCAGCAGTC TCTTCTGTTA CAGAGTCCGT AGAAACAAAC 
CTTCATCTCG ATCAAAATTA TCCTTACGGC TCTCCTGTTC GTTCGTCTAA AAATCCCTTT 
CTCTCTTCAA ATTCTCGATT ACCAACTGAT GATTCTTCTC ACACTGTTGG TTCTCATTCA 
TTTACTTCAG GAACTCACCC ACCCATTGTT TCTTCTAATT CCGCCTTTAC ACTTCCCAAT 
GCTGTTACTC CTGCAGCTCA AGCACCTCTC ATACGTAGTG TTAGTGAATA TCCTGCTAAC 
GTTTCTCCAC CTGCCCAGTC CCTTCAGCTA CCAAAATCTA CTAGCAATCC CGCGGATCTT 
CATTTAAGCA TAGCTTCTGC TAGCAGCCAT AAGAATATTT TTTCTGGGTT GGACGTCTTT 
TCTAATGTCT TTCATGGTCC CTCTACCACA TTACGAGACA GAGAACATGA TATGCGAAAT 
CGCTCATTTG ATCATTCTAC CTTGGCTCAT TATGAAGCAG TTAAGCAGCA GAGACTTGGT 
GTTGAACCTA CTGCAAGGTC CTTTACGTTG AGCTCTTATA AGTCCCGTGC AAGTGGAAAT 
TCCCTAATTA ATGATCGAAG CTCTACAACA ACGCCGACGT TTGTTAATTC GGAAGCTAGC 
TCGCCTGTTC ATAAAAATAA ACGAAGACGC AGAATATATG CTGCCTTACT TTCTCGTGTT 
GCCAGCGAAT TGTTAGATAG ACTACAGCTA GGTGATATCA CGAAAGACGG TTTAATTTAT 
TCTAATGCCT TTACTGGCGA TCATGCAGTC ACCGTGCTAA TGGGTATTAT ACATACTTCT 
GATAGAAATC TCGCTTTACT TGTCGGACGC TCTTTAGATG CACAAAAATT TATTCATGAT 
GTTACCTATG ATCATCGTCT TCGTGATTCT CATCGTGAAA TATATCAGCT TCAAGGTACT 
GGTTATCGCC CCTTCTTACG CGCAAATGAT AATGCATCCA TTAACAACAA AAACCATCAT 
AAGGAATTGG AGGACAATGA GTCGGGCACT CGAATCTCAC CGTCCACGTT AGGTGACACT 
TCTTTTCCAA ATGGTATATT TACTTTATTG ACGCATTGTT ACTCTCCAAC ATGTGCAAAA 
GATCATCCCT GCTATTCTAT TTCATGTCCT CGACGATTGG AACAGCAGCA CAGACTATTT 
GCCAAAATGC GTGCAAACAC TGAACAGTCT TCCTCCCTAG CGTTTGATGA TAAGGAACAA 
AAATTGTGGA TTCACAGCGT ACCCCAGGAG ATTGCTTATA GTGTCAGTGA CCGGGAAAGA 
AAGCGTCAAG AAGTAATTTG TGAGGTTATC TATACTGAGC GAGATTTTGT TAAGGATTTG 
GAATATCTTC GGGATTATTG GATTAAACCA CTATGGGCTT CATCTTGTAT ACCTGAACGA 
AAAAAAGAAA AATTTATTCG TACTGTGTTT TTAAATGCTT TAGAGGTTCA AGCAGTTAAT 
TCTAAATTGG CTGAAGCTCT TACTAAAAGA CAAAACTATA AACCCATTGT GGATAATATT 
GCGGATATAT TTTTGGAGCA CGTTCCAAAA TTTGAGCCTT TCATACGATA TGGTGCAGGC 
CAACTCTACG GAAAATACGA GTTTGAGAAG GAAAAGTCAT CTAATCCTGC ATTTGCTAAG 
TTTGTCAGTG ACGTTGAACG TCTGAAAGAA TCACGAAAGC TAGAGCTAAA CGGCTATCTG 
ACTAAACCAA CTACCAGATT GGCTCGTTAT CCATTACTTC TAGAAGCGGT ATTAAAATAT 
ACTGATGAGG GTAACCCTGA TAAACAAGAC ATTCCGAAGG TCATTAATAT CGTTAGAGGA 
TTTCTCTCAC GTCTTAATGT TGAAAGCGGA AAAGCAGAAA ATAAGTTTAA CTTGTTTCAT 
TTGAATCAAC AGCTGGTCTT TAAACCTGGA GAACATTATG ATTTACATCT GCTTGATGCG 
AACAGACAAT TAATATTCAA AGGACCCTTG AAGAAAAGAA GTGCTGGTTC CACTTCTTCA 
GAGTCTGCGT CGGATGTTAC TTTGTTTCTC TTTGACCATG CTTTGTTGAT AGTAAAACCA 
AAGACTATTA ATAAACGTGA ATTATTGAAA GTTTTTCAAA GACCTATTCC TCTATTACTT 
CTTCAACTAT TCCTTGTTGA TGATAATGGA TTAAGAATCC CTTATTCAAG TAAGCAACAA 
CTAGCAGCTG TATCAAAAGC AGCGAATGGG AAGCCACCTT CTAGATTTTA TCCATTTAGC 
TTACAACTTT TGGGTCGTAG AGGGTACGAA ATCACTCTTT ATGCAACCAC TGAAGTTAGT 
AGGGATAAGT GGTTAGAGCA TATTGACAAC CAACAAACAT TGCTACAACA TCGTAACCAG 
TGGTTTGAGT CTGTTACCAT TTGCTCTAAT TTCTTTGTTG GTGATAATAA AGTAAATGCG 
ATAGGCGTTT ATGATAGTGG TCGTCGACTT TTATATGGGA CTGACACAGG TGTGTACGTC 
TCTCTTCGAA AAGCAAACAG CCCACTTCAA TTCAAGCCTG TACGTGCGTT AAATATTCCC 
AACATTTCAC AGCTTGAGGT TATTGAAGAG TATAGTTTAT TGCTACTGCT TTCCGATAAG 
GTGTTATATT CGTATCCTTT GGAAATGATT GATGCGGATA CTACTCAAGC CCCTAAAAAA 
GCAAGGAAAG TTTCTGGTCA TACAACTTTC TTTCGCGTAG GAATTTGTCT GGGTAAAGTT 
CTTGTGTGTG CTGTCAAAAG TTCCGTCTTG AGTGCTACTA TAAAGGTTTT TGAACCAGTC 
ACTAATTACT CTAAAACACG GAACATGCCA AGCTTGAAAA AATTCCTTAC TGTTAACCAA 
GACCCATTGA GGATTGTCAA AGAGCTTTAT ATACCCACGG AATCAACTTC GGTTCACTTC 
CTGAAGAATA AACTCTGTGT CGGATGTACG AGAGGTTTTG AGGTGGTCAG TCTCGATAAC 
CTAGAGACGC AGTCTCTACT GGATCCAGCC GATACGTCTC TTGAATTTGT GGAGAAGAAA 
GAAAATGTTA AACCTATCGC TATCTATCGT ATGAATGGCG GTGAATTTCT TTTATGCTAT 
TCCCAATTTG CTTTTTACGT AAATCGAGAT GGATGGCGTT CTCGTCCTAC ATGGTTTGTT 
GTTTGGGAAG GAAGTCCTCA AAACTTTGCC TTAAGTTATC CTTATATATT GGCTTTTGAA 
CCCACTTTTA TTGAAATCAG ACATGTTGAA ACCTCTGAAT TGATCCATGT CATTTCCGGC 
AGAAACATCC GTTTGCTTGC TGATGGAAGA GGAAAGTTAG GGGACGGTGG TGAAATATTT 
TATGCGTGCG ATCAACGTGG TGAAAATTGT GAGACAAGTG TTGTTTGTTC CCTTCGATTG 
ACGAGTGCTG CTGCCCACGC CAAAGAGCAG CATGTAGACA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rgf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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