rgf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following rgf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rgf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99698 NM_001023106.2
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Schizosaccharomyces pombe RhoGEF family guanine nucleotide exchange factor Rgf1 (rgf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99698
    Clone ID Related Accession (Same CDS sequence) NM_001023106.2
    Accession Version NM_001023106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product RhoGEF family guanine nucleotide exchange factor Rgf1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023106.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATTACC GGCATCCAAA TGCACTCGGT GTGAATGAGA GCTCGCGTGC TTATGAGGAA 
    ATTTTTGGTG CTCCAAGAAA ACGGGAGCCG GCGAGAACTG TAAGTACTCC TGCGTTCATG
    GAACCGGCTC CAGTCTCAAA AAAACCTCTT CCTCCTCCCA CGCGAAGGCT TCCTCGGAAA
    CCTTTGCCCT TTCGTTCCAC GAGTTTGCAG CCTCCATCGT CTCAACCTCC TGCACCTCCA
    ACTCATCAGA GAGAAGCATC TCCTGTGAAA AATATTGAGC ACAGTGAATC TTTTCCTTCC
    GTTTTTGGTA CTTCAAATAA TCACCAGATC GTCCCATTGA CTCTTAAAGA CGGAAATGAT
    TTTGGTGCTC TATATGCATC TTTAAACACC ACACCCCATT TTCCTCAGGT ATCGAATCAT
    GCGCCCAATA ACTCTAATTC CCCTTCTTTG ACTTGGCACA CATCATCCGG TGATGATTCC
    AACCAAAATC CTTTTTTCGT TCGGCGTCAG TCTCAGAGTA GCACGTCTCC GGTTTCGGAT
    TCAGTAGATG AGAACCTCCT TTCAGCAGTC TCTTCTGTTA CAGAGTCCGT AGAAACAAAC
    CTTCATCTCG ATCAAAATTA TCCTTACGGC TCTCCTGTTC GTTCGTCTAA AAATCCCTTT
    CTCTCTTCAA ATTCTCGATT ACCAACTGAT GATTCTTCTC ACACTGTTGG TTCTCATTCA
    TTTACTTCAG GAACTCACCC ACCCATTGTT TCTTCTAATT CCGCCTTTAC ACTTCCCAAT
    GCTGTTACTC CTGCAGCTCA AGCACCTCTC ATACGTAGTG TTAGTGAATA TCCTGCTAAC
    GTTTCTCCAC CTGCCCAGTC CCTTCAGCTA CCAAAATCTA CTAGCAATCC CGCGGATCTT
    CATTTAAGCA TAGCTTCTGC TAGCAGCCAT AAGAATATTT TTTCTGGGTT GGACGTCTTT
    TCTAATGTCT TTCATGGTCC CTCTACCACA TTACGAGACA GAGAACATGA TATGCGAAAT
    CGCTCATTTG ATCATTCTAC CTTGGCTCAT TATGAAGCAG TTAAGCAGCA GAGACTTGGT
    GTTGAACCTA CTGCAAGGTC CTTTACGTTG AGCTCTTATA AGTCCCGTGC AAGTGGAAAT
    TCCCTAATTA ATGATCGAAG CTCTACAACA ACGCCGACGT TTGTTAATTC GGAAGCTAGC
    TCGCCTGTTC ATAAAAATAA ACGAAGACGC AGAATATATG CTGCCTTACT TTCTCGTGTT
    GCCAGCGAAT TGTTAGATAG ACTACAGCTA GGTGATATCA CGAAAGACGG TTTAATTTAT
    TCTAATGCCT TTACTGGCGA TCATGCAGTC ACCGTGCTAA TGGGTATTAT ACATACTTCT
    GATAGAAATC TCGCTTTACT TGTCGGACGC TCTTTAGATG CACAAAAATT TATTCATGAT
    GTTACCTATG ATCATCGTCT TCGTGATTCT CATCGTGAAA TATATCAGCT TCAAGGTACT
    GGTTATCGCC CCTTCTTACG CGCAAATGAT AATGCATCCA TTAACAACAA AAACCATCAT
    AAGGAATTGG AGGACAATGA GTCGGGCACT CGAATCTCAC CGTCCACGTT AGGTGACACT
    TCTTTTCCAA ATGGTATATT TACTTTATTG ACGCATTGTT ACTCTCCAAC ATGTGCAAAA
    GATCATCCCT GCTATTCTAT TTCATGTCCT CGACGATTGG AACAGCAGCA CAGACTATTT
    GCCAAAATGC GTGCAAACAC TGAACAGTCT TCCTCCCTAG CGTTTGATGA TAAGGAACAA
    AAATTGTGGA TTCACAGCGT ACCCCAGGAG ATTGCTTATA GTGTCAGTGA CCGGGAAAGA
    AAGCGTCAAG AAGTAATTTG TGAGGTTATC TATACTGAGC GAGATTTTGT TAAGGATTTG
    GAATATCTTC GGGATTATTG GATTAAACCA CTATGGGCTT CATCTTGTAT ACCTGAACGA
    AAAAAAGAAA AATTTATTCG TACTGTGTTT TTAAATGCTT TAGAGGTTCA AGCAGTTAAT
    TCTAAATTGG CTGAAGCTCT TACTAAAAGA CAAAACTATA AACCCATTGT GGATAATATT
    GCGGATATAT TTTTGGAGCA CGTTCCAAAA TTTGAGCCTT TCATACGATA TGGTGCAGGC
    CAACTCTACG GAAAATACGA GTTTGAGAAG GAAAAGTCAT CTAATCCTGC ATTTGCTAAG
    TTTGTCAGTG ACGTTGAACG TCTGAAAGAA TCACGAAAGC TAGAGCTAAA CGGCTATCTG
    ACTAAACCAA CTACCAGATT GGCTCGTTAT CCATTACTTC TAGAAGCGGT ATTAAAATAT
    ACTGATGAGG GTAACCCTGA TAAACAAGAC ATTCCGAAGG TCATTAATAT CGTTAGAGGA
    TTTCTCTCAC GTCTTAATGT TGAAAGCGGA AAAGCAGAAA ATAAGTTTAA CTTGTTTCAT
    TTGAATCAAC AGCTGGTCTT TAAACCTGGA GAACATTATG ATTTACATCT GCTTGATGCG
    AACAGACAAT TAATATTCAA AGGACCCTTG AAGAAAAGAA GTGCTGGTTC CACTTCTTCA
    GAGTCTGCGT CGGATGTTAC TTTGTTTCTC TTTGACCATG CTTTGTTGAT AGTAAAACCA
    AAGACTATTA ATAAACGTGA ATTATTGAAA GTTTTTCAAA GACCTATTCC TCTATTACTT
    CTTCAACTAT TCCTTGTTGA TGATAATGGA TTAAGAATCC CTTATTCAAG TAAGCAACAA
    CTAGCAGCTG TATCAAAAGC AGCGAATGGG AAGCCACCTT CTAGATTTTA TCCATTTAGC
    TTACAACTTT TGGGTCGTAG AGGGTACGAA ATCACTCTTT ATGCAACCAC TGAAGTTAGT
    AGGGATAAGT GGTTAGAGCA TATTGACAAC CAACAAACAT TGCTACAACA TCGTAACCAG
    TGGTTTGAGT CTGTTACCAT TTGCTCTAAT TTCTTTGTTG GTGATAATAA AGTAAATGCG
    ATAGGCGTTT ATGATAGTGG TCGTCGACTT TTATATGGGA CTGACACAGG TGTGTACGTC
    TCTCTTCGAA AAGCAAACAG CCCACTTCAA TTCAAGCCTG TACGTGCGTT AAATATTCCC
    AACATTTCAC AGCTTGAGGT TATTGAAGAG TATAGTTTAT TGCTACTGCT TTCCGATAAG
    GTGTTATATT CGTATCCTTT GGAAATGATT GATGCGGATA CTACTCAAGC CCCTAAAAAA
    GCAAGGAAAG TTTCTGGTCA TACAACTTTC TTTCGCGTAG GAATTTGTCT GGGTAAAGTT
    CTTGTGTGTG CTGTCAAAAG TTCCGTCTTG AGTGCTACTA TAAAGGTTTT TGAACCAGTC
    ACTAATTACT CTAAAACACG GAACATGCCA AGCTTGAAAA AATTCCTTAC TGTTAACCAA
    GACCCATTGA GGATTGTCAA AGAGCTTTAT ATACCCACGG AATCAACTTC GGTTCACTTC
    CTGAAGAATA AACTCTGTGT CGGATGTACG AGAGGTTTTG AGGTGGTCAG TCTCGATAAC
    CTAGAGACGC AGTCTCTACT GGATCCAGCC GATACGTCTC TTGAATTTGT GGAGAAGAAA
    GAAAATGTTA AACCTATCGC TATCTATCGT ATGAATGGCG GTGAATTTCT TTTATGCTAT
    TCCCAATTTG CTTTTTACGT AAATCGAGAT GGATGGCGTT CTCGTCCTAC ATGGTTTGTT
    GTTTGGGAAG GAAGTCCTCA AAACTTTGCC TTAAGTTATC CTTATATATT GGCTTTTGAA
    CCCACTTTTA TTGAAATCAG ACATGTTGAA ACCTCTGAAT TGATCCATGT CATTTCCGGC
    AGAAACATCC GTTTGCTTGC TGATGGAAGA GGAAAGTTAG GGGACGGTGG TGAAATATTT
    TATGCGTGCG ATCAACGTGG TGAAAATTGT GAGACAAGTG TTGTTTGTTC CCTTCGATTG
    ACGAGTGCTG CTGCCCACGC CAAAGAGCAG CATGTAGACA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588116.1
    CDS53..4057
    Translation

    Target ORF information:

    RefSeq Version NM_001023106.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe RhoGEF family guanine nucleotide exchange factor Rgf1 (rgf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATTACC GGCATCCAAA TGCACTCGGT GTGAATGAGA GCTCGCGTGC TTATGAGGAA 
    ATTTTTGGTG CTCCAAGAAA ACGGGAGCCG GCGAGAACTG TAAGTACTCC TGCGTTCATG
    GAACCGGCTC CAGTCTCAAA AAAACCTCTT CCTCCTCCCA CGCGAAGGCT TCCTCGGAAA
    CCTTTGCCCT TTCGTTCCAC GAGTTTGCAG CCTCCATCGT CTCAACCTCC TGCACCTCCA
    ACTCATCAGA GAGAAGCATC TCCTGTGAAA AATATTGAGC ACAGTGAATC TTTTCCTTCC
    GTTTTTGGTA CTTCAAATAA TCACCAGATC GTCCCATTGA CTCTTAAAGA CGGAAATGAT
    TTTGGTGCTC TATATGCATC TTTAAACACC ACACCCCATT TTCCTCAGGT ATCGAATCAT
    GCGCCCAATA ACTCTAATTC CCCTTCTTTG ACTTGGCACA CATCATCCGG TGATGATTCC
    AACCAAAATC CTTTTTTCGT TCGGCGTCAG TCTCAGAGTA GCACGTCTCC GGTTTCGGAT
    TCAGTAGATG AGAACCTCCT TTCAGCAGTC TCTTCTGTTA CAGAGTCCGT AGAAACAAAC
    CTTCATCTCG ATCAAAATTA TCCTTACGGC TCTCCTGTTC GTTCGTCTAA AAATCCCTTT
    CTCTCTTCAA ATTCTCGATT ACCAACTGAT GATTCTTCTC ACACTGTTGG TTCTCATTCA
    TTTACTTCAG GAACTCACCC ACCCATTGTT TCTTCTAATT CCGCCTTTAC ACTTCCCAAT
    GCTGTTACTC CTGCAGCTCA AGCACCTCTC ATACGTAGTG TTAGTGAATA TCCTGCTAAC
    GTTTCTCCAC CTGCCCAGTC CCTTCAGCTA CCAAAATCTA CTAGCAATCC CGCGGATCTT
    CATTTAAGCA TAGCTTCTGC TAGCAGCCAT AAGAATATTT TTTCTGGGTT GGACGTCTTT
    TCTAATGTCT TTCATGGTCC CTCTACCACA TTACGAGACA GAGAACATGA TATGCGAAAT
    CGCTCATTTG ATCATTCTAC CTTGGCTCAT TATGAAGCAG TTAAGCAGCA GAGACTTGGT
    GTTGAACCTA CTGCAAGGTC CTTTACGTTG AGCTCTTATA AGTCCCGTGC AAGTGGAAAT
    TCCCTAATTA ATGATCGAAG CTCTACAACA ACGCCGACGT TTGTTAATTC GGAAGCTAGC
    TCGCCTGTTC ATAAAAATAA ACGAAGACGC AGAATATATG CTGCCTTACT TTCTCGTGTT
    GCCAGCGAAT TGTTAGATAG ACTACAGCTA GGTGATATCA CGAAAGACGG TTTAATTTAT
    TCTAATGCCT TTACTGGCGA TCATGCAGTC ACCGTGCTAA TGGGTATTAT ACATACTTCT
    GATAGAAATC TCGCTTTACT TGTCGGACGC TCTTTAGATG CACAAAAATT TATTCATGAT
    GTTACCTATG ATCATCGTCT TCGTGATTCT CATCGTGAAA TATATCAGCT TCAAGGTACT
    GGTTATCGCC CCTTCTTACG CGCAAATGAT AATGCATCCA TTAACAACAA AAACCATCAT
    AAGGAATTGG AGGACAATGA GTCGGGCACT CGAATCTCAC CGTCCACGTT AGGTGACACT
    TCTTTTCCAA ATGGTATATT TACTTTATTG ACGCATTGTT ACTCTCCAAC ATGTGCAAAA
    GATCATCCCT GCTATTCTAT TTCATGTCCT CGACGATTGG AACAGCAGCA CAGACTATTT
    GCCAAAATGC GTGCAAACAC TGAACAGTCT TCCTCCCTAG CGTTTGATGA TAAGGAACAA
    AAATTGTGGA TTCACAGCGT ACCCCAGGAG ATTGCTTATA GTGTCAGTGA CCGGGAAAGA
    AAGCGTCAAG AAGTAATTTG TGAGGTTATC TATACTGAGC GAGATTTTGT TAAGGATTTG
    GAATATCTTC GGGATTATTG GATTAAACCA CTATGGGCTT CATCTTGTAT ACCTGAACGA
    AAAAAAGAAA AATTTATTCG TACTGTGTTT TTAAATGCTT TAGAGGTTCA AGCAGTTAAT
    TCTAAATTGG CTGAAGCTCT TACTAAAAGA CAAAACTATA AACCCATTGT GGATAATATT
    GCGGATATAT TTTTGGAGCA CGTTCCAAAA TTTGAGCCTT TCATACGATA TGGTGCAGGC
    CAACTCTACG GAAAATACGA GTTTGAGAAG GAAAAGTCAT CTAATCCTGC ATTTGCTAAG
    TTTGTCAGTG ACGTTGAACG TCTGAAAGAA TCACGAAAGC TAGAGCTAAA CGGCTATCTG
    ACTAAACCAA CTACCAGATT GGCTCGTTAT CCATTACTTC TAGAAGCGGT ATTAAAATAT
    ACTGATGAGG GTAACCCTGA TAAACAAGAC ATTCCGAAGG TCATTAATAT CGTTAGAGGA
    TTTCTCTCAC GTCTTAATGT TGAAAGCGGA AAAGCAGAAA ATAAGTTTAA CTTGTTTCAT
    TTGAATCAAC AGCTGGTCTT TAAACCTGGA GAACATTATG ATTTACATCT GCTTGATGCG
    AACAGACAAT TAATATTCAA AGGACCCTTG AAGAAAAGAA GTGCTGGTTC CACTTCTTCA
    GAGTCTGCGT CGGATGTTAC TTTGTTTCTC TTTGACCATG CTTTGTTGAT AGTAAAACCA
    AAGACTATTA ATAAACGTGA ATTATTGAAA GTTTTTCAAA GACCTATTCC TCTATTACTT
    CTTCAACTAT TCCTTGTTGA TGATAATGGA TTAAGAATCC CTTATTCAAG TAAGCAACAA
    CTAGCAGCTG TATCAAAAGC AGCGAATGGG AAGCCACCTT CTAGATTTTA TCCATTTAGC
    TTACAACTTT TGGGTCGTAG AGGGTACGAA ATCACTCTTT ATGCAACCAC TGAAGTTAGT
    AGGGATAAGT GGTTAGAGCA TATTGACAAC CAACAAACAT TGCTACAACA TCGTAACCAG
    TGGTTTGAGT CTGTTACCAT TTGCTCTAAT TTCTTTGTTG GTGATAATAA AGTAAATGCG
    ATAGGCGTTT ATGATAGTGG TCGTCGACTT TTATATGGGA CTGACACAGG TGTGTACGTC
    TCTCTTCGAA AAGCAAACAG CCCACTTCAA TTCAAGCCTG TACGTGCGTT AAATATTCCC
    AACATTTCAC AGCTTGAGGT TATTGAAGAG TATAGTTTAT TGCTACTGCT TTCCGATAAG
    GTGTTATATT CGTATCCTTT GGAAATGATT GATGCGGATA CTACTCAAGC CCCTAAAAAA
    GCAAGGAAAG TTTCTGGTCA TACAACTTTC TTTCGCGTAG GAATTTGTCT GGGTAAAGTT
    CTTGTGTGTG CTGTCAAAAG TTCCGTCTTG AGTGCTACTA TAAAGGTTTT TGAACCAGTC
    ACTAATTACT CTAAAACACG GAACATGCCA AGCTTGAAAA AATTCCTTAC TGTTAACCAA
    GACCCATTGA GGATTGTCAA AGAGCTTTAT ATACCCACGG AATCAACTTC GGTTCACTTC
    CTGAAGAATA AACTCTGTGT CGGATGTACG AGAGGTTTTG AGGTGGTCAG TCTCGATAAC
    CTAGAGACGC AGTCTCTACT GGATCCAGCC GATACGTCTC TTGAATTTGT GGAGAAGAAA
    GAAAATGTTA AACCTATCGC TATCTATCGT ATGAATGGCG GTGAATTTCT TTTATGCTAT
    TCCCAATTTG CTTTTTACGT AAATCGAGAT GGATGGCGTT CTCGTCCTAC ATGGTTTGTT
    GTTTGGGAAG GAAGTCCTCA AAACTTTGCC TTAAGTTATC CTTATATATT GGCTTTTGAA
    CCCACTTTTA TTGAAATCAG ACATGTTGAA ACCTCTGAAT TGATCCATGT CATTTCCGGC
    AGAAACATCC GTTTGCTTGC TGATGGAAGA GGAAAGTTAG GGGACGGTGG TGAAATATTT
    TATGCGTGCG ATCAACGTGG TGAAAATTGT GAGACAAGTG TTGTTTGTTC CCTTCGATTG
    ACGAGTGCTG CTGCCCACGC CAAAGAGCAG CATGTAGACA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L