ORF » Species Summary » Schizosaccharomyces pombe » sec8 cDNA ORF clone

sec8 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following sec8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99024 NM_001355880.1
Latest version!		 Schizosaccharomyces pombe exocyst complex subunit Sec8 (sec8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OSj00744 NM_001022839.2
Latest version!		 Schizosaccharomyces pombe 972h- exocyst complex subunit Sec8 (sec8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    sec8 ( NM_001355880.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342752.1 Schizosaccharomyces pombe exocyst complex subunit Sec8 (sec8), mRNA.
    Price & Availability↑
    CloneID OSc99024
    Clone ID Related Accession (Same CDS sequence) NM_001355880.1
    Accession Version NM_001355880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product exocyst complex subunit Sec8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
    TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
    GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
    GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
    TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
    AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
    GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
    CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
    ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
    GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
    AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
    GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
    TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
    TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
    AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
    GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
    GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
    TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
    ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
    CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
    TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
    TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
    ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
    AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
    AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
    ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
    ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
    GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
    GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
    GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
    AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
    GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
    ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
    ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
    TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
    CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
    GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
    CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
    CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
    CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
    GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
    AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
    GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
    TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
    ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
    GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
    ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
    GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
    AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
    ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
    TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
    TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
    AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
    CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342752.1
    CDS58..3279
    Translation

    Target ORF information:

    RefSeq Version NM_001355880.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe exocyst complex subunit Sec8 (sec8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001355880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
    TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
    GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
    GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
    TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
    AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
    GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
    CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
    ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
    GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
    AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
    GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
    TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
    TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
    AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
    GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
    GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
    TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
    ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
    CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
    TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
    TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
    ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
    AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
    AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
    ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
    ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
    GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
    GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
    GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
    AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
    GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
    ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
    ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
    TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
    CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
    GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
    CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
    CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
    CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
    GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
    AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
    GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
    TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
    ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
    GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
    ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
    GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
    AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
    ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
    TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
    TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
    AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
    CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    sec8 ( NM_001022839.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587846.2 Schizosaccharomyces pombe 972h- exocyst complex subunit Sec8 (sec8), mRNA.
    Price & Availability↑
    CloneID OSj00744
    Clone ID Related Accession (Same CDS sequence) NM_001022839.2
    Accession Version NM_001022839.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product exocyst complex subunit Sec8
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022839.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
    TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
    GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
    GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
    TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
    AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
    GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
    CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
    ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
    GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
    AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
    GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
    TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
    TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
    AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
    GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
    GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
    TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
    ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
    CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
    TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
    TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
    ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
    AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
    AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
    ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
    ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
    GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
    GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
    GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
    AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
    GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
    ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
    ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
    TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
    CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
    GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
    CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
    CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
    CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
    GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
    AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
    GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
    TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
    ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
    GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
    ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
    GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
    AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
    ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
    TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
    TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
    AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
    CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587846.2
    CDS58..3279
    Translation

    Target ORF information:

    RefSeq Version NM_001022839.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- exocyst complex subunit Sec8 (sec8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022839.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
    TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
    GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
    GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
    TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
    AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
    GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
    CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
    ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
    GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
    AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
    GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
    TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
    TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
    AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
    GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
    GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
    TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
    ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
    CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
    TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
    TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
    ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
    AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
    AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
    ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
    ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
    GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
    GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
    GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
    AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
    GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
    ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
    ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
    TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
    CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
    GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
    CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
    CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
    CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
    GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
    AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
    GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
    TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
    ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
    GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
    ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
    GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
    AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
    ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
    TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
    TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
    AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
    CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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