sec8 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec8
Entrez Gene ID	2539407
Full Name	exocyst complex subunit Sec8
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022839.2	NP_587846.2	exocyst complex subunit Sec8
NM_001355880.1	NP_001342752.1	exocyst complex subunit Sec8

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E11419g	XP_454464.1
Saccharomyces cerevisiae (baker's yeast)	SEC8	NP_015380.1
Schizosaccharomyces pombe (fission yeast)	sec8	NP_587846.2
Magnaporthe oryzae (rice blast fungus)	MGG_03985	XP_003719868.1
Neurospora crassa	NCU04190	XP_961150.2
Eremothecium gossypii	AGOS_ADL317C	NP_983779.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sec8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99024	NM_001355880.1 Latest version!	Schizosaccharomyces pombe exocyst complex subunit Sec8 (sec8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSj00744	NM_001022839.2 Latest version!	Schizosaccharomyces pombe 972h- exocyst complex subunit Sec8 (sec8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec8 ( NM_001355880.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342752.1 Schizosaccharomyces pombe exocyst complex subunit Sec8 (sec8), mRNA.

Price & Availability↑

CloneID	OSc99024
Clone ID Related Accession (Same CDS sequence)	NM_001355880.1
Accession Version	NM_001355880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	exocyst complex subunit Sec8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421).

ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342752.1
CDS	58..3279
Translation	MDTRGYSETKKGRYPVGKKSLESPNGYSNYGTSMDNELSSEYASRGMHIGDLENLVNEIEDEWKDLGREDYQPISTALELLDDSSFGRDYKSFLNVYDRISAALQTIAHTHKDDFTRGISAYGEIMEGIQKCNSRIIALKQSLEASQECIGNTNSKELQQTLARSSQYKKVISVLKELNEANQLFDNFHTLVDSKQYYHASDLIRRVWDELSRSDFDGILVVEQFKSRMTGLLSHLEDILSEELVSITFLKDAVAYPIVSYCSPNPLRETSNPYFLRDFLKNNANTSTLGQSEQLRYLEEALSLKLSDCLKMDYGRDSLRDIRIVLESLNLLGKLPNAISSLKSRTSAEMFTTVDSTSRAIVNKYSLGNNVSTVNPFSKSLYDIGLHAETDREHTMISEFLTNLFTKLRCVLMHYRGISEFMTKLETKTPKHASSSHKSSIMSVNSDPTSPKVSKFDTSDSTFPFDTLLQAFESEIRLMLKDYLISKEEYIENSGNFVVGTEMSIYNLPGENEEDKLFDVTNEIAVENKSNAFYARINELVNEKAPELILNKSNASVSTIELFSGSSKEIVRLAGHVVFVGPSVFHASSVLPQTVFFLEDSVSILKNPNIPPQFAVNFMKEFLRGSYIPQLYKFMSSHFDTIMKDVGAFQLHRDWKIYSKIPIFKCHVAIVQYFHDLQDYLPIVALNLVEFYELLHTLLVRFRNHCSDYLSDLCRTAVLKEYKHVNEDTEDVDDTVRVKLLHDDVTYPQFIKFLKQKNPSLEGLNELCRMENKRLLQYEDRAITSEVKLPVSVLSKDSDLVNSVSYLHNSMEWFLQRCFSRFMNGSRRMNVLQQNQANFGGDFLPIDNLLGNNSDLMKGAYKEVFDSLQRLQFDALLLIRMEVRLQYIHSINQSVNLPDYVVEYRGRPDASIMALNSTIVTTNLKLETCLNEWERRFVFQGLSELVDSSLYSIFYKIESMNRGSCLQMLKNMSAMIQILKTVKEIHGDVEFPKSSRVFGIYQNGAKKIIEHFIAAPKKELLPDVKQMVRIYYQRLMKDAKRNGRDDLYRQYQKKIGSVLTQFDNTVGGARKNP

Target ORF information:

RefSeq Version	NM_001355880.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe exocyst complex subunit Sec8 (sec8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355880.1

ORF Insert Sequence:

ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec8 ( NM_001022839.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587846.2 Schizosaccharomyces pombe 972h- exocyst complex subunit Sec8 (sec8), mRNA.

Price & Availability↑

CloneID	OSj00744
Clone ID Related Accession (Same CDS sequence)	NM_001022839.2
Accession Version	NM_001022839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	exocyst complex subunit Sec8
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022839.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_587846.2
CDS	58..3279
Translation	MDTRGYSETKKGRYPVGKKSLESPNGYSNYGTSMDNELSSEYASRGMHIGDLENLVNEIEDEWKDLGREDYQPISTALELLDDSSFGRDYKSFLNVYDRISAALQTIAHTHKDDFTRGISAYGEIMEGIQKCNSRIIALKQSLEASQECIGNTNSKELQQTLARSSQYKKVISVLKELNEANQLFDNFHTLVDSKQYYHASDLIRRVWDELSRSDFDGILVVEQFKSRMTGLLSHLEDILSEELVSITFLKDAVAYPIVSYCSPNPLRETSNPYFLRDFLKNNANTSTLGQSEQLRYLEEALSLKLSDCLKMDYGRDSLRDIRIVLESLNLLGKLPNAISSLKSRTSAEMFTTVDSTSRAIVNKYSLGNNVSTVNPFSKSLYDIGLHAETDREHTMISEFLTNLFTKLRCVLMHYRGISEFMTKLETKTPKHASSSHKSSIMSVNSDPTSPKVSKFDTSDSTFPFDTLLQAFESEIRLMLKDYLISKEEYIENSGNFVVGTEMSIYNLPGENEEDKLFDVTNEIAVENKSNAFYARINELVNEKAPELILNKSNASVSTIELFSGSSKEIVRLAGHVVFVGPSVFHASSVLPQTVFFLEDSVSILKNPNIPPQFAVNFMKEFLRGSYIPQLYKFMSSHFDTIMKDVGAFQLHRDWKIYSKIPIFKCHVAIVQYFHDLQDYLPIVALNLVEFYELLHTLLVRFRNHCSDYLSDLCRTAVLKEYKHVNEDTEDVDDTVRVKLLHDDVTYPQFIKFLKQKNPSLEGLNELCRMENKRLLQYEDRAITSEVKLPVSVLSKDSDLVNSVSYLHNSMEWFLQRCFSRFMNGSRRMNVLQQNQANFGGDFLPIDNLLGNNSDLMKGAYKEVFDSLQRLQFDALLLIRMEVRLQYIHSINQSVNLPDYVVEYRGRPDASIMALNSTIVTTNLKLETCLNEWERRFVFQGLSELVDSSLYSIFYKIESMNRGSCLQMLKNMSAMIQILKTVKEIHGDVEFPKSSRVFGIYQNGAKKIIEHFIAAPKKELLPDVKQMVRIYYQRLMKDAKRNGRDDLYRQYQKKIGSVLTQFDNTVGGARKNP

Target ORF information:

RefSeq Version	NM_001022839.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- exocyst complex subunit Sec8 (sec8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022839.2

ORF Insert Sequence:

ATGGATACCA GAGGCTATTC GGAAACGAAA AAAGGAAGGT ATCCAGTGGG AAAGAAAAGC 
TTGGAATCTC CTAACGGCTA CTCAAATTAT GGAACTAGCA TGGACAATGA ATTGAGTTCA 
GAGTATGCAT CGCGTGGAAT GCACATAGGC GATTTAGAAA ATTTAGTAAA TGAAATTGAG 
GATGAATGGA AAGACCTGGG AAGAGAAGAC TATCAACCGA TATCTACAGC GTTGGAACTA 
TTAGATGACA GTAGCTTTGG AAGAGATTAT AAGTCTTTTC TGAACGTTTA CGATCGAATT 
AGTGCTGCAT TACAAACGAT TGCCCATACT CATAAAGATG ACTTTACTCG AGGTATATCG 
GCTTATGGAG AAATAATGGA GGGAATTCAG AAGTGCAATT CGAGAATCAT AGCATTAAAG 
CAATCCCTTG AGGCTTCGCA AGAATGCATT GGTAATACTA ATAGCAAAGA GCTTCAACAA 
ACATTGGCTC GTAGTTCTCA ATACAAAAAA GTCATAAGTG TGCTCAAGGA ACTTAATGAA 
GCCAACCAGC TGTTTGACAA CTTTCATACG CTTGTTGATA GCAAGCAATA TTATCATGCC 
AGCGATTTAA TACGTCGTGT ATGGGATGAA TTAAGTCGTT CTGATTTTGA CGGAATATTA 
GTAGTGGAGC AGTTTAAAAG TCGGATGACA GGACTCTTGT CACATTTAGA GGACATTCTA 
TCTGAAGAGT TGGTTTCAAT TACTTTCCTT AAAGATGCTG TCGCTTATCC CATAGTATCG 
TACTGCTCTC CAAATCCGCT GAGGGAAACT TCCAACCCAT ATTTTTTGAG AGATTTTTTA 
AAAAACAATG CAAATACATC AACTCTGGGC CAAAGTGAGC AATTGCGTTA CCTAGAAGAA 
GCGCTTTCTT TAAAGTTGTC GGATTGCTTA AAAATGGATT ATGGTAGAGA CAGTTTACGT 
GATATACGTA TTGTTTTGGA GTCATTGAAT TTATTGGGAA AACTCCCCAA TGCTATTTCA 
TCTTTAAAGT CTCGTACTTC TGCGGAGATG TTTACTACGG TAGATTCTAC TTCACGTGCA 
ATCGTTAACA AATATTCACT CGGTAATAAC GTTTCTACCG TTAATCCCTT TTCAAAATCA 
CTTTATGATA TAGGTTTGCA CGCTGAGACT GATAGAGAGC ATACTATGAT TTCCGAGTTC 
TTGACGAACT TATTTACAAA ATTAAGGTGT GTTTTAATGC ATTATCGCGG TATCTCCGAA 
TTCATGACCA AATTAGAGAC TAAAACACCA AAGCATGCTT CATCTTCTCA CAAATCTAGC 
ATTATGTCTG TTAACTCGGA CCCCACAAGC CCAAAGGTAT CAAAATTTGA TACTTCTGAT 
AGCACGTTTC CATTTGATAC GTTGCTGCAA GCATTCGAGA GCGAAATTAG GTTAATGTTA 
AAAGATTATC TTATTTCCAA AGAAGAGTAT ATTGAAAATA GTGGAAATTT CGTTGTTGGA 
ACTGAGATGT CCATATACAA CCTCCCTGGC GAAAATGAAG AAGATAAACT TTTCGACGTA 
ACAAACGAAA TTGCTGTGGA GAACAAAAGT AATGCGTTTT ATGCCAGAAT AAATGAGTTG 
GTTAACGAAA AGGCTCCAGA GCTTATTCTT AATAAATCAA ATGCTTCAGT TTCCACTATT 
GAACTATTTT CTGGAAGTTC GAAAGAAATT GTACGTTTGG CTGGCCACGT TGTATTTGTT 
GGGCCTTCGG TTTTTCATGC TTCGTCTGTC CTACCTCAAA CCGTATTCTT TTTGGAAGAC 
AGTGTGTCTA TTTTGAAAAA CCCAAATATA CCTCCTCAGT TTGCTGTTAA TTTTATGAAA 
GAATTTTTAA GGGGAAGTTA CATTCCACAA TTATACAAGT TTATGTCGAG TCACTTTGAC 
ACCATAATGA AAGACGTGGG TGCGTTCCAA TTGCACAGAG ATTGGAAGAT ATACTCTAAA 
ATCCCAATTT TTAAGTGCCA TGTGGCTATT GTTCAATATT TTCACGATCT TCAAGATTAT 
TTGCCTATTG TTGCATTAAA TTTGGTAGAA TTTTATGAAT TATTACATAC TTTGCTTGTT 
CGTTTTCGCA ACCATTGTTC TGATTATCTC TCTGATTTGT GCAGAACGGC TGTTCTCAAA 
GAATACAAAC ATGTCAACGA GGATACTGAA GATGTCGATG ATACTGTTCG AGTAAAGCTA 
CTTCACGACG ATGTAACGTA CCCTCAATTT ATTAAATTCC TCAAGCAAAA AAATCCATCA 
CTTGAAGGGT TAAATGAGCT TTGTCGAATG GAGAATAAGC GTTTATTACA ATATGAAGAT 
CGTGCGATTA CGTCCGAAGT TAAACTTCCG GTATCGGTTT TATCCAAAGA TTCCGACCTA 
GTGAACTCGG TTTCTTATCT TCATAACTCC ATGGAATGGT TTCTTCAGCG CTGTTTTTCA 
AGATTTATGA ACGGTTCGCG AAGAATGAAC GTTTTGCAAC AAAACCAAGC TAATTTCGGT 
GGTGACTTTT TACCAATTGA TAATTTGTTG GGTAATAATT CGGATTTAAT GAAGGGTGCA 
TATAAAGAAG TCTTTGATTC TTTACAGAGG CTCCAATTCG ATGCTTTACT GCTAATCCGA 
ATGGAAGTTC GGTTACAGTA CATTCATAGT ATTAATCAAA GTGTAAACCT TCCTGACTAT 
GTTGTCGAGT ACCGCGGAAG ACCAGATGCT TCGATAATGG CTCTCAACTC TACTATTGTG 
ACGACGAATT TGAAACTCGA AACCTGTTTG AATGAATGGG AACGAAGGTT TGTATTCCAA 
GGTCTGAGTG AACTGGTTGA TTCATCGCTA TACTCTATCT TTTACAAAAT AGAATCAATG 
AATAGAGGCA GCTGTCTTCA GATGCTGAAA AATATGTCAG CAATGATTCA GATTCTTAAG 
ACGGTTAAGG AAATTCATGG CGACGTTGAA TTTCCTAAAT CTTCTAGAGT CTTTGGAATA 
TACCAAAATG GTGCCAAGAA AATTATCGAG CACTTCATTG CTGCCCCCAA AAAGGAACTT 
TTGCCTGACG TTAAACAGAT GGTCCGAATT TATTACCAGC GATTAATGAA AGATGCAAAA 
AGAAACGGAA GGGACGATCT TTATCGGCAG TATCAGAAAA AGATTGGCTC GGTTTTAACT 
CAGTTCGACA ACACTGTGGG TGGTGCGAGA AAAAATCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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オンライン注文

sec8 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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