ORF » Species Summary » Schizosaccharomyces pombe » pef1 cDNA ORF clone

pef1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following pef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99376 NM_001022912.1
Latest version!		 Schizosaccharomyces pombe Pho85/PhoA-like cyclin-dependent kinase Pef1 (pef1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pef1 ( NM_001022912.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587921.1 Schizosaccharomyces pombe Pho85/PhoA-like cyclin-dependent kinase Pef1 (pef1), partial mRNA.
    Price & Availability↑
    CloneID OSc99376
    Clone ID Related Accession (Same CDS sequence) NM_001022912.1
    Accession Version NM_001022912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product Pho85/PhoA-like cyclin-dependent kinase Pef1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAACTACC AAAGGCTTGA AAAGTTAGGA GAGGGAACAT ATGCGCATGT TTATAAGGGG 
    CAAAATCGAG TGACTGGTGA AATTGTCGCT CTCAAAGTTA TTCGTATTGA TGCAGATGAA 
    GGAACACCTA GTACTGCTAT CCGTGAGATT AGTCTTATGA AGGAGCTTAG GCATCCCAAT 
    ATTATGTCTC TCTCAGACGT TTTGCAAACC GAAAATAAGC TTATGCTCGT TTTTGAATAT 
    ATGGAAAAAG ATTTGAAAAA GTACATGGAC ACTTATGGGA ACCAAGGAGC TCTTCCACCT 
    TCTCAAGTAA AAAATTTTAC TCAACAATTA CTCAAAGGGA TATCTTTTTG TCACGAAAAC 
    CGAGTTCTAC ATCGTGATCT GAAACCTCAA AATTTGCTTA TCAACAGTCG TGGTGAATTG 
    AAATTGGCGG ATTTTGGGTT AGCCCGTTCA ATTGGAATTC CAGTGAATAC TTTTTCAAAC 
    GAAGTGGTCA CCTTATGGTA TCGTGCTCCT GACGTTCTTT TGGGTTCTCG AGTATATTCA 
    ACTTCCATTG ATATTTGGTC AGTTGGTTGT ATCATGGCAG AAATGGCTAC AGGACGTCCT 
    CTATTTGCTG GGTCAAATAA TGAGGATCAG CTACTAAAAA TCTTCCGATT ATTGGGAACT 
    CCTACTGAAC AATCATGGCC CGGAATTTCT TTACTTCCTG AATATAAGCC TACTTTCCCT 
    ATTTATAAAG CACAAGATCT CGCCTACCTA TTTCCAACAT TTGATCCTCT TGGTTTGGAT 
    TTGTTACGAC GGATGCTTCG TCTACAACCT GAATTAAGGA CGACCGGCCA GGATGCGCTT 
    CAACATGCTT GGTTTTTAAC CGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587921.1
    CDS1..867
    Translation

    Target ORF information:

    RefSeq Version NM_001022912.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe Pho85/PhoA-like cyclin-dependent kinase Pef1 (pef1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAACTACC AAAGGCTTGA AAAGTTAGGA GAGGGAACAT ATGCGCATGT TTATAAGGGG 
    CAAAATCGAG TGACTGGTGA AATTGTCGCT CTCAAAGTTA TTCGTATTGA TGCAGATGAA 
    GGAACACCTA GTACTGCTAT CCGTGAGATT AGTCTTATGA AGGAGCTTAG GCATCCCAAT 
    ATTATGTCTC TCTCAGACGT TTTGCAAACC GAAAATAAGC TTATGCTCGT TTTTGAATAT 
    ATGGAAAAAG ATTTGAAAAA GTACATGGAC ACTTATGGGA ACCAAGGAGC TCTTCCACCT 
    TCTCAAGTAA AAAATTTTAC TCAACAATTA CTCAAAGGGA TATCTTTTTG TCACGAAAAC 
    CGAGTTCTAC ATCGTGATCT GAAACCTCAA AATTTGCTTA TCAACAGTCG TGGTGAATTG 
    AAATTGGCGG ATTTTGGGTT AGCCCGTTCA ATTGGAATTC CAGTGAATAC TTTTTCAAAC 
    GAAGTGGTCA CCTTATGGTA TCGTGCTCCT GACGTTCTTT TGGGTTCTCG AGTATATTCA 
    ACTTCCATTG ATATTTGGTC AGTTGGTTGT ATCATGGCAG AAATGGCTAC AGGACGTCCT 
    CTATTTGCTG GGTCAAATAA TGAGGATCAG CTACTAAAAA TCTTCCGATT ATTGGGAACT 
    CCTACTGAAC AATCATGGCC CGGAATTTCT TTACTTCCTG AATATAAGCC TACTTTCCCT 
    ATTTATAAAG CACAAGATCT CGCCTACCTA TTTCCAACAT TTGATCCTCT TGGTTTGGAT 
    TTGTTACGAC GGATGCTTCG TCTACAACCT GAATTAAGGA CGACCGGCCA GGATGCGCTT 
    CAACATGCTT GGTTTTTAAC CGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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